Publications

a comparison of the lethal and hemolytic toxins of clostridium histolyticum.		1952	13006411
the activity of clostridium histolyticum proteinase on synthetic substrates.		1953	13031635
the haemolysin of clostridium histolyticum.		1953	13106224
[studies on the properties of clostridium histolyticum and clostridium septicum antisera].		1953	13124941
separation of proteolytic enzymes of clostridium histolyticum.		1954	13223777
[the proteolytic enzymes of clostridium histolyticum; influence of complexon iii and of calcium on the stability of two of these enzymes].		1955	13260245
[ammonia production and toxicity of clostridium histolyticum cultured in a gelatinated medium].		1956	13363038
pharmacologically-active polypeptide formed from blood globulin by a cysteine-activated protease from clostridium histolyticum.		1956	13373851
[not available].		1956	13395010
exopeptidases of clostridium histolyticum.		1957	13445228
nutritional studies with clostridium histolyticum.		1957	13462971
[purification and chemical properties of anatoxin of clostridium histolyticum].		1957	13499480
effects of clostridium histolyticum enzymes upon burned and unburned tissues in vitro.		1958	13521674
new proteolytic enzymes from clostridium histolyticum filtrates.		1958	13525621
the cysteine-inactivated proteinase (alpha-antigen) of clostridium histolyticum.		1958	13525622
serological evidence for a specific clostridium histolyticum gelatinase.		1958	13525623
clostridium histolyticum collagenase: its purification and properties.		1958	13534676
[studies on clostridium histolyticum anatoxins using zone electrophoresis and counter diffusion on agar].		1959	13636341
[study of immunological phenomena by means of tissue culture. iv. cytopathogenic effects of clostridium histolyticum toxin].		1959	13658453
studies on the collagenases of clostridium histolyticum.		1959	13661949
the use of clostridium histolyticum enzymes in the treatment of experimental third degree burns.		1959	13675991
[research on the odors emitted by anaerobic bacteria. iii. clostridium histolyticum].		1961	13701340
[clostripain, a protease from clostridium histolyticum. 2. specificity].		1960	13708985
[on proteins and their decomposition products. 18. the hydrolysis of synthetic and natural substrates by collagenase from clostridium histolyticum].		1960	13714094
[clostripain, a protease from clostridium histolyticum. 1. purification activation by thiols].		1960	13758027
electron microscopic studies on ultrathin sections of spores of clostridium tetani and clostridium histolyticum, with special reference to sporulation and spore germination process.		1960	13774854
factors affecting the elaboration by clostridium histolyticum of proteinases capable of debriding third degree burn eschars on guinea pigs.	berman, sanford (walter reed army institute of research, washington, d. c.), joseph p. lowenthal, marion e. webster, patricia l. altieri, and raymond b. gochenour. factors affecting the elaboration by clostridium histolyticum of proteinases capable of debriding third degree burn eschars on guinea pigs. j. bacteriol. 82:582-588. 1961.-a comparison of three strains of clostridium histolyticum grown in two different media indicated that the h-4 strain produced the greatest yield of enzyme(s) capabl ...	1961	13867979
large scale purification of proteinases from clostridium histolyticum filtrates.	conklin, david a. (walter reed army institute of research, washington, d. c.), marion e. webster, patricia l. altieri, sanford berman, joseph p. lowenthal, and raymond b. gochenour. large scale purification of proteinases from clostridium histolyticum filtrates. j. bacteriol. 82:589-594. 1961.-a method for the large scale preparation and partial purification of clostridium histolyticum proteinases by fractional precipitation with ammonium sulfate is described. conditions for adequate separation ...	1961	13880849
[experimental inoculation of the chick embryo with clostridium histolyticum].		1961	13884989
debridement of burns on various animal species by clostridium histolyticum proteinases.		1962	13899369
[stability behavior of collagenase from clostridium histolyticum].		1963	13938916
the interactions between clostridium septicum and clostridium histolyticum toxins and antitoxins.		1962	13983973
enzymatic debridement of third-degree burns on guinea pigs by clostridium histolyticum proteinases.	webster, marion e. (walter reed army institute of research, washington, d. c.), patricia l. altieri, david a. conklin, sanford berman, joseph p. lowenthal, and raymond b. gochenour. enzymatic debridement of third-degree burns on guinea pigs by clostridium histolyticum proteinases. j. bacteriol. 83:602-608. 1962.-an attempt has been made to correlate in vitro activities of clostridium histolyticum h-4 proteinases, as measured against azocasein, azocoll, gelatin, and collagen, with their ability t ...	1962	14005493
multiplicity of clostridium histolyticum collagenases.		1964	14240635
debridement of experimental burns with clostridium histolyticum h-4 enzymes.		1960	14399293
[on proteins and their reduction products. xvi. on the specificity of collagenase from clostridium histolyticum and its application to the determination of the primary structure of collagen. (preliminary report)].		1959	14401656
toxicity of enzymes from clostridium histolyticum.		1960	14403502
protease inhibitors: synthesis of bacterial collagenase and matrix metalloproteinase inhibitors incorporating succinyl hydroxamate and iminodiacetic acid hydroxamate moieties.	a series of succinyl hydroxamates/bishydroxamates as well as a new structural type of matrix metalloproteinase (mmp)/bacterial protease (bp) inhibitors, incorporating iminodiacetic (ida) hydroxamate/bishydroxamate moieties, has been synthesized and tested for interaction with four vertebrate proteases, mmp-1, mmp-2, mmp-8 and mmp-9, and a bp, the collagenase isolated from clostridium histolyticum (chc). the new derivatives generally showed inhibition constants in the range of 8-62 nm against the ...	2003	14506914
enzymes of clostridium welchii type a and clostridium histolyticum that disintegrate decalcified human tooth dentine.		1950	14778940
the alpha, beta and gamma antigens of clostridium histolyticum (weinberg & séguin, 1916).		1950	14778941
the serological identification of cultures of clostridium histolyticum as group iii of the proteolytic clostridia.		1952	14888966
the structure-function relationship in the clostripain family of peptidases.	in this study we investigate the active-site structure and the catalytic mechanism of clostripain by using a combination of three separate techniques: affinity labelling, site-directed mutagenesis and molecular modelling. a benzamidinyl-diazo dichlorotriazine dye (bdd) was shown to act as an efficient active site-directed affinity label for clostridium histolyticum clostripain. the enzyme, upon incubation with bdd in 0.1 m hepes/naoh buffer ph 7.6, exhibits a time-dependent loss of activity. the ...	2004	15009210
[the influences of lanthanum,cerium and fluorine ions to the activity of collagenase degrading cemental collagens]	the influences of trace elements lanthanum,cerium and fluorine to the activity of clostridium histolyticum collagenase(chc) during the period of chc degrading the cemental root collagen in vitro is studied.the results reveal that la(3+) can significantly inhibit the activity of the chc than ce(3+) and f(-). therefore it suggests that lanthanum may be used as a new kind of element in preventing root caries.the mechanisms of above functions are also discussed.	1997	15159961
inhibition effects of (+)-catechin-aldehyde polycondensates on proteinases causing proteolytic degradation of extracellular matrix.	inhibition effects of (+)-catechin-aldehyde polycondensates against the activity of proteinases, clostridium histolyticum collagenase (chc) and human neutrophil elastase (hne) causing proteolytic degradation of extracellular matrix (ecm), have been investigated. in normal tissues, a balance is reached between the formation and destruction of ecm, leading to a state of homeostasis. however, uncontrolled destruction of ecm contributes to tumor invasion and metastasis. in the measurement of the inh ...	2004	15207729
quantitative analysis of the impact of the organic matrix on the fluoride effect on erosion progression in human dentine using longitudinal microradiography.	after an initial demineralisation, an intensive fluoridation is capable of inhibiting the erosive mineral loss in dentine completely, which might be related to the presence of the exposed organic dentine matrix. aim of the present study was, therefore, to evaluate whether fluoride is also effective when the organic material is removed. the study was a cyclic de- and remineralisation model over 5 days in vitro. samples from human coronal dentine were demineralised with citric acid (ph 2.3; 6 x 10 ...	2004	15353250
interaction of aldehydes with collagen: effect on thermal, enzymatic and conformational stability.	stabilization of type i rat tail tendon (rtt) collagen by various aldehydes, viz. formaldehyde, gluteraldehyde, glyoxal and crotanaldehyde was studied to understand the effect of each on the thermal, enzymatic and conformational stability of collagen. the aldehydes have been found to increase the heat stability of rat tail tendon collagen fibres from 62 to 77-86 degrees c. the increase in thermal stability was found to be in a species dependent manner. the variation in the thermal stability of c ...	2004	15374680
outbreak of clostridium histolyticum infections in injecting drug users in england and scotland.	clostridial infections in injecting drug users in the united kingdom are a relatively new phenomenon that came to light in 2000 when cases of serious illness and deaths due to clostridium novyi were recorded. in the period december 2003 to april 2004, the anaerobe reference laboratory received twelve referrals of an extremely rare isolate, clostridium histolyticum, from cases of infection in injecting drug users submitted from nine different hospitals in england and scotland. molecular typing of ...	2004	15381836
production, purification, and some properties of clostridium histolyticum collagenase.		1950	15440885
factor xiii contrasts the effects of metalloproteinases in human dermal fibroblast cultured cells.	matrix metalloproteinases (mmps) are overexpressed in venous leg ulcers, determining a breakdown of the main extracellular matrix (ecm) components owing mainly to collagenase activities, and so playing a crucial role in ulcer pathogenesis. the authors studied the effects of coagulation factor xiii (fxiii), which cross-links collagen and other ecm components, in human fibroblast cultured cells in the presence and in the absence of matrix metalloproteinases from clostridium histolyticum collagenas ...	2004	15490040
quantum theoretic qsar of benzene derivatives: some enzyme inhibitors.	our previously developed approach to the development of qsar equations for benzene derivatives, originally for phenylalkylamine hallucinogens, has been applied to four new systems: sulfonamide inhibitors of the enzymes carbonic anhydrase, thrombin, trypsin, and clostridium histolyticum collagenase. the novel features involve the energies and nodal orientations of pi-like orbitals, and an allowance for the symmetry of the benzene nucleus. the resulting equations give better fits, better predictiv ...	2004	15499995
improved enzymatic isolation of fibroblasts for the creation of autologous skin substitutes.	the number of medical applications using autologous fibroblasts is increasing rapidly. we investigated thoroughly the procedure to isolate cells from skin using the enzymatic tissue dissociation procedure. tissue digestion efficiency, cell viability, and yield were investigated in relation to size of tissue fragments, digestion volume to tissue ratio, digestion time, and importance of other protease activities present in clostridium histolyticum collagenase (chc) (neutral protease, clostripain, ...	2004	15723562
an unusual cause of soft tissue infection.		2005	15951543
modulation of the fecal microbiota by the intake of a lactobacillus johnsonii la1-containing product in human volunteers.	lactobacillus johnsonii la1 (la1) is a probiotic strain capable of stimulating the immune system of the host and interfering with gastrointestinal pathogens. this study evaluates how the ingestion of different amounts of la1 influences the main bacterial populations of the fecal microbiota. eight asymptomatic volunteers participated in the study. after a basal period, they ingested daily 100 ml of a product containing 10(8) cfu ml(-1) of la1 during the first week, 200 ml during the second week a ...	2005	15970400
a novel galactooligosaccharide mixture increases the bifidobacterial population numbers in a continuous in vitro fermentation system and in the proximal colonic contents of pigs in vivo.	prebiotics are nondigestible food ingredients that encourage proliferation of selected groups of the colonic microflora, thereby altering the composition toward a more beneficial community. in the present study, the prebiotic potential of a novel galactooligosaccharide (gos) mixture, produced by the activity of galactosyltransferases from bifidobacterium bifidum 41171 on lactose, was assessed in vitro and in a parallel continuous randomized pig trial. in situ fluorescent hybridization with 16s r ...	2005	15987856
[substrate specificity of collagenase of streptomyces sp. 1349 and keratinase of streptomyces sp. 1382].	the paper determines the action specificity concerning the bonding type of collagenases of strepomyces sp. 1349 and keratinases of streptomyces sp. 1382. experimental data obtained evidence for the wide specificity of the obtained enzymatic drugs. it has been established that both collagenases and keratinases display high specificity in respect of the bonds made by the residues of hydrophobic amino acids. collagenases of streptomyces sp. 1349, as to their wide action specificity, differed from c ...	2005	16018211
determination of the cytotoxic effect of clostridium histolyticum culture supernatant on hela cells in the presence of protease inhibitors.	clostridium histolyticum culture supernatant contains numerous enzymes, which exert a cytotoxic effect on host cells. this includes lethal toxin, clostripain and high-potassium-sensitive toxin. since the number of c. histolyticum infections increased during the last several years, it seems worthwhile to evaluate whether protease inhibitors, used for the treatment of many diseases, could influence toxicity, and thus, pathogenicity of c. histolyticum. in this study we evaluated in vitro the influe ...	2005	16051065
differences between the gut microflora of children with autistic spectrum disorders and that of healthy children.	children with autistic spectrum disorders (asds) tend to suffer from severe gastrointestinal problems. such symptoms may be due to a disruption of the indigenous gut flora promoting the overgrowth of potentially pathogenic micro-organisms. the faecal flora of patients with asds was studied and compared with those of two control groups (healthy siblings and unrelated healthy children). faecal bacterial populations were assessed through the use of a culture-independent technique, fluorescence in s ...	2005	16157555
inhibition of collagenase by naturally-occurring flavonoids.	we examined the inhibitory activities of various flavonoids, including the flavanones, flavones/isoflavones and flavonols, on collagenase from clostridium histolyticum to establish their therapeutic potential against skin inflammation and photoaging. in general, the flavonols were stronger inhibitors than the flavones/isoflavones, and this indicated the importance of the c-3 hydroxyl substitution. quercetin was the most active flavonoid among those tested, and it showed an ic50 of 286 microm. th ...	2005	16276971
new worlds to explore.		2006	16439327
effect of a milk formula with prebiotics on the intestinal microbiota of infants after an antibiotic treatment.	antibiotics exert deleterious effects on the intestinal microbiota, favoring the emergence of opportunistic bacteria and diarrhea. prebiotics are nondigestible food components that stimulate the growth of bifidobacteria. our aim was to evaluate the effects on the intestinal microbiota of a prebiotic-supplemented milk formula after an antibiotic treatment. a randomized, double-blind, controlled clinical trial was carried out in 140 infants 1-2 y of age distributed into two groups after a 1-wk amo ...	2006	16492988
vacuolization of hela cells by a partially purified clostridium histolyticum cytotoxin.	clostridium histolyticum vacuolating cytotoxin was partially purified from culture broth using ammonium sulfate precipitation, gel filtration and hydrophobic interaction chromatography. the toxin caused vacuolization of hela cells visible under a light microscope after 2 h and distinct after 8 h. transmission electron microscopy revealed the presence of numerous vacuoles, condensation of the mitochondrial matrix, increased cytoplasm density and increased amounts of heterochromatin. apoptosis was ...	2006	16553808
proteolysis and the selective destruction of amino acids by clostridium sporogenes and clostridium histolyticum.		1929	16559359
variability in morphological and biochemical properties of clostridium histolyticum (weinberg and seguin).		1937	16560062
some serological aspects of the s-r change in clostridium histolyticum.		1937	16560063
prevalence and temporal stability of selected clostridial groups in irritable bowel syndrome in relation to predominant faecal bacteria.	the differences in faecal bacterial population between irritable bowel syndrome (ibs) and control subjects have been reported in several studies. the aim of the present study was to compare the predominant and clostridial faecal microbiota of ibs subjects and healthy controls by applying denaturing gradient gel electrophoresis (dgge) and a recently developed multiplexed and quantitative hybridization-based technique, transcript analysis with the aid of affinity capture (trac). according to the r ...	2006	16585652
qsar analysis of some 5-amino-2-mercapto-1,3,4-thiadiazole based inhibitors of matrix metalloproteinases and bacterial collagenase.	a quantitative structure-activity relationship (qsar) study has been performed on 5-amino-2-mercapto-1,3,4-thiadiazole based inhibitors of matrix metalloproteinases (mmps) and a bacterial collagenase known as clostridium histolyticum collagenase (chc) to understand the structural features influencing the affinity of these inhibitors towards the enzyme. the compounds in the selected series were characterized by topological and fragmental descriptors calculated using quasar module of molecular ope ...	2006	16682189
studies on the proteinase of clostridium histolyticum.		1937	16746452
proteinase secretion and growth of clostridium histolyticum.		1938	16746798
studies on the endo-enzymes, particularly the peptidases, of clostridium histolyticum.		1938	16746799
enzymic adaptation in clostridium histolyticum.		1938	16746800
the proteinases of clostridium histolyticum.		1940	16747287
molecular characterisation of the gut microflora of healthy and inflammatory bowel disease cats using fluorescence in situ hybridisation with special reference to desulfovibrio spp.	inflammatory bowel disease (ibd) is a common cause of chronic large bowel diarrhoea in cats. although the aetiology of ibd is unknown, an immune-mediated response to a luminal antigen is thought to be involved. as knowledge concerning the colonic microflora of cats is limited and requires further investigation, the purpose of this study was to determine the presence of specific bacterial groups in normal and ibd cats, and the potential role they play in the health of the host. total bacterial po ...	2007	17217390
factors influencing the collagenase digestion phase of human islet isolation.	substantial advances in human islet isolation technology have occurred during the past decade. however, it is still difficult to recover the entire quantity of islets contained in a pancreas. a major obstacle to successful human islet isolation has been the variability of the collagenase digestion phase of islet isolation. future advances in enzyme technology will make it possible to optimally liberate islets with enzyme blends "tailor-made" for each individual donor pancreas. such innovative st ...	2007	17220782
analysis of contribution of collagen fibre component in viscoelastic behaviour of periodontal ligament using enzyme probe.	the aims of this study are to observe microscopic changes in the periodontal ligament (pdl) collagen fibres after collagenase treatment, to analyse stress-relaxation behaviour of pdl specimens treated with collagenase, and to elucidate the contribution of the collagen component to the viscoelastic behaviour of the pdl. transverse sections of rat mandibular first molars (n=24) were treated in vitro with 0, 8, 16, or 24 units of bacterial collagenase for 4h at 37 degrees c. histological specimens ...	2007	17266965
lethal factor of clostridium histolyticum kills cells by apoptosis.	in this study, for the first time, clostridium histolyticum lethal factor was purified by ammonium sulfate precipitation of culture broth, centrifugation through an amicon filter device and hydrophobic interaction chromatography. the purified lethal factor was devoid of proteolytic activity. at a concentration of 150 ng ml(-1) the lethal factor killed 50% of hela cells within 24 h of exposure. abrogation of actin filaments, activation of caspases, fragmentation of nuclear dna as well as ultrastr ...	2007	17328764
jerusalem artichoke and chicory inulin in bakery products affect faecal microbiota of healthy volunteers.	a study was conducted to test the effects of jerusalem artichoke inulin (ja) or chicory inulin (ch) in snack bars on composition of faecal microbiota, concentration of faecal scfa, bowel habit and gastrointestinal symptoms. forty-five volunteers participated in a double-blind, randomized, placebo-controlled, parallel-group study. at the end of a 7 d run-in period, subjects were randomly assigned to three groups of fifteen subjects each, consuming either snack bars with ch or ja, or snack bars wi ...	2007	17445348
differences between the fecal microbiota of coeliac infants and healthy controls.	coeliac disease (cd) is an immune-mediated enteropathy with a multifactorial aetiology, characterized by chronic inflammation of the small intestinal mucosa. although evidence suggests that the gut microbiota contributes to other chronic inflammatory disorders, its possible role in cd has not been determined. in this study, the composition of the fecal microbiota of coeliac children and age-matched controls was investigated by culture-dependent and -independent methodologies, using fluorescent i ...	2007	17489434
effect of bacterial collagenase on resin-dentin bonds degradation.	the objective of this study is to evaluate the effect of a bacterial collagenase on the degradation of resin-dentin bonds. human dentin surfaces were bonded with: an etch-&-rinse self-priming adhesive (sb), a two-step self-etching primer/adhesive (seb), and a 1-step self-etching adhesive (oub). composite build-ups were constructed. the bonded teeth were stored (24 h, 3 months, 1 year) in distilled water or in a buffered bacterial collagenase solution. half of the specimens were stored as intact ...	2007	17569004
antiadhesive effects of mitomycin c and streptopeptidase a in rats with intraperitoneal adhesions.	intraabdominal adhesion formation is a frequent problem after major abdominal surgery. for many years, there have been various attempts to decrease adhesions by using systemic and local drugs and mechanical barriers. in this study we aimed to evaluate the antifibrinolytic antiadhesive effects of mitomycin c (mmc) and streptopeptidase a (sa) against intraabdominal adhesions. forty-eight rats were divided into six groups, each with eight rats. group 1 (sham group) rats were laparotomized by transv ...	2007	17579872
in vitro binding of matrix metalloproteinase-2 (mmp-2), mmp-9, and bacterial collagenase on collagenous wound dressings.	chronic wounds are characterized by failure in wound-healing response and a delay in healing or nonclosure of the wounds. this results in a high effort in clinical treatment and/or home care. a major difference between acute wounds and chronic wounds is the imbalance of proteinase inhibitors and proteinase activity that regulates the degradation and regeneration of the extracellular matrix proteins. collagen and collagen/oxidized regenerated cellulose dressings act as a competitive substrate for ...	2007	17650099
structure of the claudin-binding domain of clostridium perfringens enterotoxin.	clostridium perfringens enterotoxin is a common cause of food-borne and antibiotic-associated diarrhea. the toxin's receptors on intestinal epithelial cells include claudin-3 and -4, members of a large family of tight junction proteins. toxin-induced cytolytic pore formation requires residues in the nh(2)-terminal half, whereas residues near the cooh terminus are required for binding to claudins. the claudin-binding cooh-terminal domain is not toxic and is currently under investigation as a pote ...	2008	17977833
imbalance in the composition of the duodenal microbiota of children with coeliac disease.	coeliac disease (cd) is the most common immune-mediated enteropathy characterized by chronic inflammation of the small intestinal mucosa. the ingestion of gluten is responsible for the symptoms of cd, but other environmental factors are also thought to play a role in this disorder. in this study, the composition of the duodenal microbiota of coeliac children with active disease, symptom-free cd patients on a gluten-free diet and control children was determined. bacteriological analyses of duoden ...	2007	18033837
characterization of the gastrointestinal mucosa-associated microbiota of pigs and chickens using culture-based and molecular methodologies.	the microbiota of the gastrointestinal tract (git) can play an important role in the healthy status of farm animals and in the safety of the whole food chain. in this study, the mucosa-associated microbiota of the git of pigs and chickens was analyzed by culture methods and fluorescence in situ hybridization combined with flow cytometry (fcm-fish). in all pig git sections, lactic acid bacteria, enterobacteriaceae, atopobium, bacteroides, and clostridium histolyticum were the predominant bacteria ...	2007	18095433
clostridium histolyticum infection.		1949	18146655
a method of isolating the collagen (i) alpha2 chain carboxytelopeptide for species identification in bone fragments.	we present a novel method for the isolation and analysis of the bone collagen (i) alpha2 chain carboxytelopeptide as a species biomarker. conventional methods for the analysis and sequencing of mixtures of proteins and peptides commonly involve using the protease trypsin to cleave proteins present in the sample. however, in the study of collagen, these methods result in very complex mixtures of peptides that are difficult to analyze and the acquired results are not reproducible. here we use bact ...	2008	18164255
an internally quenched fluorescent substrate for collagenase.	a synthetic collagenase substrate containing the internal peptide sequence--gly-gly-pro-leu-gly-pro-pro-gly-pro--has been synthesized, with an n-terminus 4-((4-(dimethylamino)phenyl)azo)-benzoyl (dabcyl) group and c-terminus 5-[2-(acetamido)ethylamino] naphthalene-1-sulfonic acid (aedans) moiety resulting in internal quenching of aedans fluorescence. peptide bond hydrolysis results in a large increase in fluorescence at 490 nm upon excitation at 336 nm. the substrate is cleaved exclusively by cl ...	2008	18260138
development and characterization of a collagen degradation assay to assess purified collagenase used in islet isolation.	a collagen degradation activity (cda) assay was developed to improve the biochemical characterization of purified collagenase used for islet isolation.	2008	18374061
in vitro fermentation of oat and barley derived beta-glucans by human faecal microbiota.	fermentation of beta-glucan fractions from barley [average molecular mass (mm), of 243, 172, and 137 kda] and oats (average mm of 230 and 150 kda) by the human faecal microbiota was investigated. fractions were supplemented to ph-controlled anaerobic batch culture fermenters inoculated with human faecal samples from three donors, in triplicate, for each substrate. microbiota changes were monitored by fluorescent in situ hybridization; groups enumerated were: bifidobacterium genus, bacteroides an ...	2008	18430007
crystallization and preliminary x-ray characterization of the catalytic domain of collagenase g from clostridium histolyticum.	the catalytic domain of collagenase g from clostridium histolyticum has been cloned, recombinantly expressed in escherichia coli and purified using affinity and size-exclusion column-chromatographic methods. crystals of the catalytic domain were obtained from 0.12 m sodium citrate and 23%(v/v) peg 3350 at 293 k. the crystals diffracted to 2.75 a resolution using synchrotron radiation. the crystals belong to an orthorhombic space group, with unit-cell parameters a = 57, b = 109, c = 181 a. this u ...	2008	18453715
molecular analysis of the digestive microbiota in a gnotobiotic mouse model during antibiotic treatment: influence of saccharomyces boulardii.	the probiotic saccharomyces boulardii is a non-pathogenic yeast that has been proven efficient in the prevention of antimicrobial-associated diarrhea and of clostridium difficile associated colitis. we evaluated the influence of the administration of s. boulardii on the composition of the fecal microbiota in a human microbiota-associated mouse model. this evaluation was run before, during and after a 7-day oral treatment with amoxicillin clavulanic acid. predominant groups of bacteria were quant ...	2008	18511310
broad-range pcr as a supplement to culture for detection of bacterial pathogens in patients with a clinically diagnosed spinal infection.	we aimed to evaluate broad-range pcr and subsequent sequencing compared to conventional culture in the diagnosis of spinal infection. the method was a prospective study of all patients admitted to aarhus university hospital for surgery during a 12-months period with a clinically diagnosed infection of the spine. samples from patients undergoing surgery for non-infectious causes (malignancy etc.) were included as control group. specimens were submitted to conventional culture and molecular invest ...	2008	18609207
high-level expression of his-tagged clostridial collagenase in clostridium perfringens.	clostridium histolyticum collagenase is used to isolate cells from various organs and tissues for tissue engineering, and also to treat destructive fibrosis; thus, the demand for high-grade enzyme preparations is increasing. in this study, we constructed a plasmid encoding c. histolyticum type ii collagenase (colh) with a c-terminal hexahistidine tag (colh-his) to facilitate the purification of the enzyme through immobilized metal affinity chromatography (imac). when colh-his was expressed in a ...	2008	18629492
[a mass-spectrometric approach to primary screening of collagenolytic enzymes].	a comparative analysis of maldi tof mass spectra of low-molecular products resulting from the hydrolysis of native collagen i by collagenases of various classes (bacterial metallocollagenase from clostridium histolyticum, serine collagenase from the morikrasa commercial preparation, cysteine collagenase from serratia proteomaculans, and cysteine collagenases from larvae of beetles dermestesfrischi and d. maculates) was carried out. the spectra contain a number of ion peaks common for all collage ...	2008	18672690
detrimental effect of excessive collagenase class ii on human islet isolation outcome.	commercially available purified collagenases, derived from clostridium histolyticum, contain two different classes of collagenase: class i collagenase (ci) and class ii collagenase (cii) at a predetermined ratio. in this study, using purified ci and cii in separate vials, we had a unique opportunity to investigate the effect of the proportion between two collagenase classes on clinical human islet isolation. pancreas organs derived from deceased donors were prospectively assigned to one of three ...	2008	18680482
experimental model of warfarin-associated intracerebral hemorrhage.	future demographic changes predict an increase in the number of patients with atrial fibrillation. as long-term anticoagulation for the prevention of ischemic strokes becomes more prevalent, the burden of warfarin-associated intracerebral hemorrhage (w-ich) is likely to grow. however, little is known about the clinical aspects and pathophysiologic mechanisms of w-ich. this study describes the development of a mouse model of w-ich in which hematoma growth and outcomes can be correlated with antic ...	2008	18772448
the activation mechanism and physicochemical properties of clostridium histolyticum proteinase.		1948	18871209
biochemical characterization of the catalytic domains of three different clostridial collagenases.	clostridial collagenases are used for a broad spectrum of biotechnological applications and represent prime target candidates for both therapy and diagnosis of clostridial infections. in this study, we biochemically characterized the catalytic domains of three clostridial collagenases, collagenase g (colg) and h (colh) from clostridium histolyticum, and collagenase t (colt) from c. tetani. all protein samples showed activity against a synthetic peptidic substrate (furylacryloyl-leu-gly-pro-ala, ...	2009	18937627
shifts in clostridia, bacteroides and immunoglobulin-coating fecal bacteria associated with weight loss in obese adolescents.	to evaluate the effects of a multidisciplinary obesity treatment programme on fecal microbiota composition and immunoglobulin-coating bacteria in overweight and obese adolescents and their relationship to weight loss.	2009	19050675
[molecular diagnosis of osteogenesis imperfecta type i].	osteogenesis imperfecta (oi) is caused by mutations in collagen type i genes. in contrast to oi type ii, iii and iv where there are the structural mutations, in oi type i decreased production of normal collagen is due to the presence of a null allele. because both pharmacological and gene therapy approaches depend on type of mutation and its consequences, quick and proper diagnosis is required.	2008	19145934
unidirectional binding of clostridial collagenase to triple helical substrates.	histotoxic clostridia produce collagenases responsible for extensive tissue destruction in gas gangrene. the c-terminal collagen-binding domain (cbd) of these enzymes is the minimal segment required to bind to collagen fibril. collagen binding efficiency of cbd is more pronounced in the presence of ca(2+). we have shown that cbd can be functional to anchor growth factors in local tissue. a (1)h-(15)n hsqc nmr titration study with three different tropocollagen analogues ((pog)(10))(3), ((gpog)(7) ...	2009	19208618