| single-site substitutions improve cold activity and increase thermostability of the dehairing alkaline protease (dhap). | to engineer dehairing alkaline protease (dhap) variants to improve cold activity and increase thermostability so these variants are suitable for the leather processing industry. based on previous studies with bacterial alkaline proteases, double-site mutations (w106k/v149i and w106k/m124l) were introduced into the dhap from bacillus pumilus. compared with the wild-type dhap hydrolytic activity, the double-site variant w106k/v149i showed an increase in specific hydrolytic activity at 15 °c by 2.3 ... | 2016 | 27609316 |
| residue y70 of the nitrilase cyanide dihydratase from bacillus pumilus is critical for formation and activity of the spiral oligomer. | nitrilases pose attractive alternatives to the chemical hydrolysis of nitrile compounds. the activity of bacterial nitrilases towards substrate is intimately tied to the formation of large spiral-shaped oligomers. in the nitrilase cynd (cyanide dihydratase) from bacillus pumilus, mutations in a predicted oligomeric surface region altered its oligomerization and reduced its activity. one mutant, cynd y70c, retained uniform oligomer formation however it was inactive, unlike all other inactive muta ... | 2016 | 27586531 |
| bacterial populations associated with smokeless tobacco products. | there are an estimated 8 million users of smokeless tobacco products (stps) in the united states, and yet limited data on microbial populations within these products exist. to better understand the potential microbiological risks associated with stp use, a study was conducted to provide a baseline microbiological profile of stps. a total of 90 samples, representing 15 common stps, were purchased in metropolitan areas in little rock, ar, and washington, dc, in november 2012, march 2013, and july ... | 2016 | 27565615 |
| genome sequence of a multidrug-resistant strain of bacillus pumilus, cb01, isolated from the feces of an american crow, corvus brachyrhynchos. | avian species have the potential to serve as important reservoirs for the spread of pathogenic microorganisms. here, we report the genome sequence of a drug-resistant strain of bacillus pumilus, cb01, isolated from the feces of an american crow, corvus brachyrhynchos. | 2016 | 27540060 |
| an alkaline protease from bacillus pumilus mp 27: functional analysis of its binding model toward its applications as detergent additive. | a proteolytic strain of bacillus pumilus mp 27 was isolated from water samples of southern ocean produced alkaline protease. since protease production need expensive ingredients, an economically viable process was developed by using low cost carbon source, wheat straw, supplemented with peptone. this protease was active within temperature ranges 10-70°c at ph 9. this process was optimized by response surface methodology using a box bekhman design by design expert 7.0 software that increased the ... | 2016 | 27536284 |
| how the intricate interactions between carbon nanotubes and two bilirubin oxidases control direct and mediated o2 reduction. | due to the lack of a valid approach in the design of electrochemical interfaces modified with enzymes for efficient catalysis, many oxidoreductases are still not addressed by electrochemistry. we report in this work an in-depth study of the interactions between two different bilirubin oxidases, (from the fungus myrothecium verrucaria and from the bacterium bacillus pumilus), catalysts of oxygen reduction, and carbon nanotubes bearing various surface charges (pristine, carboxylic-, and pyrene-met ... | 2016 | 27533778 |
| increased mrna expression of selected antimicrobial peptides around ovulation and during inflammatory processes in the bovine endometrium postpartum. | in the uterus, the first pathogen confrontations take place at the luminal endometrial epithelium. therefore, it is required that these cells have the potential to recognize and respond to a bacterial infection. antimicrobial peptides (amp), part of the innate immune system in addition to cytokines, are principal effector molecules of mucosal immunity against pathogens. one important family of amp that can permeabilize bacterial membranes is the beta-defensin (defb) family, which includes the fo ... | 2016 | 27531713 |
| [a sepsis case caused by a rare opportunistic pathogen: bacillus pumilus]. | the high prevalence of bacillus species in nature and the detection of these bacteria as contaminant in cultures may lead diagnostic dilemma, however they should still be considered as a pathogen particularly in case of repeated positive cultures from patients with risk factors. bacillus pumilus is a bacteria, though rarely, been reported as the causative agent of various infections such as sepsis, endocarditis, skin infections and food poisoning in human. in this report, a sepsis case in an imm ... | 2016 | 27525402 |
| transfer of radiocesium from rhizosphere soil to four cruciferous vegetables in association with a bacillus pumilus strain and root exudation. | this study was carried out to assess the effect of bacillus pumilus on the roots of four cruciferous vegetables with different root structures in regard to enhancement of (137)cs bioavailability in contaminated rhizosphere soil. results revealed that b. pumilus inoculation did not enhance the plant biomass of vegetables, although it increased root volume and root surface areas of all vegetables except turnip. the ph changes due to rhizosphere acidification by b. pumilus inoculation and root exud ... | 2016 | 27517724 |
| extracellular proteome profiling of bacillus pumilus scu11 producing alkaline protease for dehairing. | bacillus pumilus is one of the most characterized microorganisms that are used for high-level production of select industrial enzymes. a novel b. pumilus scu11 strain possessing high alkaline protease activity was obtained in our previous work. the culture supernatant of this strain showed efficient dehairing capability with minimal collagen damage, indicating promising potential applications in the leather industry. in this study, the strain's extracellular proteome was identified by lc-ms/ms-b ... | 2016 | 27470280 |
| draft genome sequence of bacillus pumilus ku-bf1 isolated from the gut contents of wood boring mesomorphus sp. | | 2016 | 27446065 |
| affinity-driven immobilization of proteins to hematite nanoparticles. | functional nanoparticles are valuable materials for energy production, bioelectronics, and diagnostic devices. the combination of biomolecules with nanosized material produces a new hybrid material with properties that can exceed the ones of the single components. hematite is a widely available material that has found application in various sectors such as in sensing and solar energy production. we report a single-step immobilization process based on affinity and achieved by genetically engineer ... | 2016 | 27429157 |
| [combined action of binase and bleomycin toward human lung adenocarcinoma cells]. | some microbial ribonucleases (rnases) demonstrate selective cytotoxic effect against a wide range of tumor cells. in this context combined use of cytotoxic rnases in complex therapy with other chemotherapeutic agents appears to be especially promising. in this study we have investigated the apoptosis-induced effect of bacillus pumilus rnase (binase) in combination with known anti-tumor antibiotic bleomycin on human lung adenocarcinoma a549 cells. the combined effect of high concentrations of the ... | 2016 | 27420619 |
| identification of two new keratinolytic proteases from a bacillus pumilus strain using protein analysis and gene sequencing. | the bacillus strain (ccug 66887) has a high capacity to excrete keratinase with the ability to degrade both alpha- and beta keratin. in this study we aimed to show the characteristics of the keratinolytic protease and to identify its gene by using liquid chromatography-electrospray ionization tandem mass spectrometry methods (nanohplc-esi-ms/ms) followed by mascot data base search. the results showed that the enzyme in fact consists of two different keratinases, both with a molecular mass of 38 ... | 2016 | 27363997 |
| bacillus pumilus safr-032 genome revisited: sequence update and re-annotation. | bacillus pumilus strain safr-032 is a non-pathogenic spore-forming bacterium exhibiting an anomalously high persistence in bactericidal environments. in its dormant state, it is capable of withstanding doses of ultraviolet (uv) radiation or hydrogen peroxide, which are lethal for the vast majority of microorganisms. this unusual resistance profile has made safr-032 a reference strain for studies of bacterial spore resistance. the complete genome sequence of b. pumilus safr-032 was published in 2 ... | 2016 | 27351589 |
| isolation, identification, and optimal cultivation of a marine bacterium antagonistic to magnaporthe grisea. | in this paper, a plate confrontation method was used to isolate bacteria antagonistic to the rice blast fungus magnaporthe grisea from samples collected from china's dalian bay. the antagonist strain lm-031 was obtained. we studied this strain's morphological, physiological and biochemical characteristics and analyzed its 16s rdna sequence. we compared the effects of different culture conditions (type of media, carbon and nitrogen source, incubation temperature and time, and initial ph value) on ... | 2016 | 27323038 |
| optimal secretion of alkali-tolerant xylanase in bacillus subtilis by signal peptide screening. | xylanases are industrially important enzymes for xylan digestion. we experimentally screened over 114 sec and 24 tat pathway signal peptides, with two different promoters, for optimal production of an alkaline active xylanase (xynbyg) from bacillus pumilus byg in a bacillus subtilis host. though both promoters yielded highly consistent secretion levels (0.97 pearson correlation coefficient), the sec pathway was found to be more efficient than the tat pathway for xynbyg secretion. furthermore, th ... | 2016 | 27225471 |
| increasing the catalytic activity of bilirubin oxidase from bacillus pumilus: importance of host strain and chaperones proteins. | aggregation of recombinant proteins into inclusion bodies (ibs) is the main problem of the expression of multicopper oxidase in escherichia coli. it is usually attributed to inefficient folding of proteins due to the lack of copper and/or unavailability of chaperone proteins. the general strategies reported to overcome this issue have been focused on increasing the intracellular copper concentration. here we report a complementary method to optimize the expression in e. coli of a promising bilir ... | 2016 | 27165502 |
| digital data for quick response (qr) codes of alkalophilic bacillus pumilus to identify and to compare bacilli isolated from lonar crator lake, india. | microbiologists are routinely engaged isolation, identification and comparison of isolated bacteria for their novelty. 16s rrna sequences of bacillus pumilus were retrieved from ncbi repository and generated qr codes for sequences (fasta format and full gene bank information). 16srrna were used to generate quick response (qr) codes of bacillus pumilus isolated from lonar crator lake (19° 58' n; 76° 31' e), india. bacillus pumilus 16s rrna gene sequences were used to generate cgr, fcgr and pca. t ... | 2016 | 27141529 |
| cationic antimicrobial peptides derived from crocodylus siamensis leukocyte extract, revealing anticancer activity and apoptotic induction on human cervical cancer cells. | known antimicrobial peptides kt2 and rt2 as well as the novel rp9 derived from the leukocyte extract of the freshwater crocodile (crocodylus siamensis) were used to evaluate the ability in killing human cervical cancer cells. rp9 in the extract was purified by a combination of anion exchange column and reversed-phase hplc, and its sequence was analyzed by mass spectrometry. the novel peptide could inhibit gram-negative vibrio cholerae (clinical isolation) and gram-positive bacillus pumilus tistr ... | 2016 | 27129462 |
| a bacillus sp. isolated from sediments of the sarno river mouth, gulf of naples (italy) produces a biofilm biosorbing pb(ii). | a pb-resistant bacterial strain (named hereinafter pb15) has been isolated from highly polluted marine sediments at the sarno river mouth, italy, using an enrichment culture to which pb(ii) 0.48mmoll(-1) were added. 16s rrna gene sequencing (sanger) allowed assignment of the isolate to the genus bacillus, with bacillus pumilus as the closest species. the isolate is resistant to pb(ii) with a minimum inhibitory concentration (mic) of 4.8mmoll(-1) and is also resistant to cd(ii) and mn(ii) with mi ... | 2016 | 27110973 |
| identification of phenazine-1-carboxylic acid gene (phc cd) from bacillus pumilus mtcc7615 and its role in antagonism against rhizoctonia solani. | bacillus pumilus mtcc7615, a biocontrol agent isolated from rice rhizosphere was characterized to be antagonistic to rhizoctonia solani, the pathogen causing sheath blight disease of rice. the phenazine-1-carboxylic acid gene (phc cd) of this bacterium was pcr amplified (1400 bp), cloned, and sequenced. the sequence analysis revealed the presence of two orfs of phc cd gene commonly found in pseudomonas species. the sequence showed 98% similarity to phc cd gene of the pseudomonas isolate lbum223 ... | 2016 | 27106067 |
| [characterization of β-1, 4-mannanase from bacillus pumilus and heterologous expression in lactobacillus casei]. | lactobacillus casei is widely used in food production and feed industry. the aim of this study was to construct the recombinant expression mannanase lb. casei. | 2015 | 27101700 |
| direct inhibition of oncogenic kras by bacillus pumilus ribonuclease (binase). | ras proteins function as molecular switches that transmit signals from cell surface receptors into specific cellular responses via activation of defined signaling pathways (fang, 2015). aberrant constitutive ras activation occurs with high incidence in different types of cancer (bos, 1989). thus, inhibition of ras-mediated signaling is extremely important for therapeutic approaches against cancer. here we showed that the ribonuclease (rnase) binase, directly interacts with endogenous kras. furth ... | 2016 | 27066977 |
| heterologous expression and characterization of a new heme-catalase in bacillus subtilis 168. | reactive oxygen species (ros) is an inherent consequence to all aerobically living organisms that might lead to the cells being lethal and susceptible to oxidative stress. bacillus pumilus is characterized by high-resistance oxidative stress that stimulated our interest to investigate the heterologous expression and characterization of heme-catalase as potential biocatalyst. results indicated that recombinant enzyme significantly exhibited the high catalytic activity of 55,784 u/mg expressed in ... | 2016 | 27016935 |
| mixed phenolic acids mediated proliferation of pathogens talaromyces helicus and kosakonia sacchari in continuously monocultured radix pseudostellariae rhizosphere soil. | radix pseudostellariae l. is a common and popular chinese medication. however, continuous monoculture has increased its susceptibility to severe diseases. we identified two pathogenic microorganisms, talaromyces helicus m. (ku355274) and kosakonia sacchari w. (ku324465), and their antagonistic bacterium, bacillus pumilus z. in rhizosphere soil of continuously monocultured r. pseudostellariae. nine types of phenolic acids were identified both in the rhizosphere soil and in culture medium under st ... | 2016 | 27014250 |
| growth and (137)cs uptake and accumulation among 56 japanese cultivars of brassica rapa, brassica juncea and brassica napus grown in a contaminated field in fukushima: effect of inoculation with a bacillus pumilus strain. | fifty six local japanese cultivars of brassica rapa (40 cultivars), brassica juncea (10 cultivars) and brassica napus (6 cultivars) were assessed for variability in growth and (137)cs uptake and accumulation in association with a bacillus pumilus strain. field trial was conducted at a contaminated farmland in nihonmatsu city, in fukushima prefecture. inoculation resulted in different responses of the cultivars in terms of growth and radiocesium uptake and accumulation. b. pumilus induced a signi ... | 2016 | 26986237 |
| [alkaline-adapted beta-mannanase of bacillus pumilus: gene heterologous expression and enzyme characterization]. | we expressed a novel alkaline-adapted beta-mannanase gene and characterized the enzyme for potential industrial applications. | 2015 | 26915226 |
| identification of bacillus probiotics isolated from soil rhizosphere using 16s rrna, reca, rpob gene sequencing and rapd-pcr. | some bacillus species, especially bacillus subtilis and bacillus pumilus groups, have highly similar 16s rrna gene sequences, which are hard to identify based on 16s rdna sequence analysis. to conquer this drawback, rpob, reca sequence analysis along with randomly amplified polymorphic (rapd) fingerprinting was examined as an alternative method for differentiating bacillus species. the 16s rrna, rpob and reca genes were amplified via a polymerase chain reaction using their specific primers. the ... | 2016 | 26898909 |
| construction and characterization of three protein-targeting expression system in lactobacillus casei. | we previously reported that the β-1,4-mannanase (manb) gene from bacillus pumilus functions as a good reporter gene in lactobacillus casei. two vectors were constructed. one carries the signal peptide of secretion protein usp45 (spusp45) from lactococcus lactis (pelsh), and the other carries the full-length s-layer protein, slpa, from l. acidophilus (pelwh). in this work, another vector, pelsph, was constructed to include the signal peptide of protein slpa (spslpa), and the capacity of all three ... | 2016 | 26892019 |
| regioselective deprotection of the monosaccharide-bearing thiocyanomethyl group at the anomeric position monitored by reversed-phase hplc method. | in the current work, the investigation and development of a chemo-enzymatic approach for the synthesis of neo-glycoproteins have been studied. this strategy is based on the regioselective enzymatic hydrolysis of peracetylated monosaccharide, functionalized at the anomeric position (c1) as 1-thio-(s-cyanomethyl) group, a precursor of the 2- iminomethoxyethyl thioglycosides-linker for protein glycosylation, catalyzed by immobilized enzymes to obtain selectively monodeprotected compounds. the use o ... | 2016 | 26864255 |
| thermostable xanthine oxidase activity from bacillus pumilus rl-2d isolated from manikaran thermal spring: production and characterization. | xanthine oxidase is an important enzyme of purine metabolism that catalyzes the hydroxylation of hypoxanthine to xanthine and then xanthine to uric acid. a thermostable xanthine oxidase is being reported from a thermophilic organism rl-2d isolated from the manikaran (kullu) hot spring of himachal pradesh (india). based on the morphology, physiological tests, and 16s rdna gene sequence, rl-2d was identified as bacillus pumilus. optimization of physiochemical parameters resulted into 4.1-fold incr ... | 2016 | 26843701 |
| cell wall as a target for bacteria inactivation by pulsed electric fields. | the integrity and morphology of bacteria is sustained by the cell wall, the target of the main microbial inactivation processes. one promising approach to inactivation is based on the use of pulsed electric fields (pef). the current dogma is that irreversible cell membrane electro-permeabilisation causes the death of the bacteria. however, the actual effect on the cell-wall architecture has been poorly explored. here we combine atomic force microscopy and electron microscopy to study the cell-wa ... | 2016 | 26830154 |
| applicability of uv resistant bacillus pumilus endospores as a human adenovirus surrogate for evaluating the effectiveness of virus inactivation in low-pressure uv treatment systems. | recent studies have demonstrated the potential to use bacillus pumilus endospores as a surrogate for human adenovirus (hadv) in uv disinfection studies. the use of endospores has been limited by observations of batch-to-batch variation in uv sensitivity. this study reports on a propagation method that utilizes a commercially available medium to produce uv tolerant b. pumilus endospores with a consistent uv sensitivity. it is further demonstrated that the endospores of b. pumilus strain (atcc 271 ... | 2016 | 26825005 |
| purification and partial characterization of serine-metallokeratinase from a newly isolated bacillus pumilus nrc21. | a serine metallokeratinase enzyme (30 kda) produced by a newly isolated bacillus strain (bacillus pumilus nrc21) cultivated under optimized conditions in medium containing chicken feather meal was purified and characterized in a set of biochemical assays. the purification was carried out using two successive chromatographic steps; cation exchange chromatography on cm-cellulose and gel filtration on sephadex g-100 columns. the purified enzyme showed a specific activity of 2000 units/mg protein ag ... | 2016 | 26802243 |
| an in-depth characterization of the entomopathogenic strain bacillus pumilus 15.1 reveals that it produces inclusion bodies similar to the parasporal crystals of bacillus thuringiensis. | in the present work, the local isolate bacillus pumilus 15.1 has been morphologically and biochemically characterized in order to gain a better understanding of this novel entomopathogenic strain active against ceratitis capitata. this strain could represent an interesting biothechnological tool for the control of this pest. here, we report on its nutrient preferences, extracellular enzyme production, motility mechanism, biofilm production, antibiotic suceptibility, natural resistance to chemica ... | 2016 | 26782747 |
| aerobic and anaerobic microbial degradation of crude (4-methylcyclohexyl)methanol in river sediments. | cyclohexane and some of its derivatives have been a major concern because of their significant adverse human health effects and widespread occurrence in the environment. the 2014 west virginia chemical spill has raised public attention to (4-methylcyclohexyl)methanol (4-mchm), one cyclohexane derivative, which is widely used in coal processing but largely ignored. in particular, the environmental fate of its primary components, cis- and trans-4-mchm, remains largely unexplored. this study aimed ... | 2016 | 26780132 |
| catalytic, kinetic and thermodynamic properties of bacillus pumilus fh9 keratinase conjugated with activated pectin. | bacillus pumilus fh9 keratinase was covalently coupled to several oxidized polysaccharides. the conjugates were evaluated for the retained activity, kinetic and thermodynamic stability. among all preparations, the conjugated enzyme with oxidized pectin had the highest recovered activity (71.75%) and the highest thermal stability at 60°c (t1/2=333 min). compared to the native enzyme, the conjugated preparation exhibited higher optimum temperature, lower activation energy (ea), lower deactivation ... | 2016 | 26743746 |
| biosynthesis and characterization of a thermostable, alkali-tolerant chitinase from bacillus pumilus jubch08 displaying antagonism against phytopathogenic fusarium oxysporum. | the present investigation highlights the process parameters influencing the submerged fermentation of chitinase by bacillus pumilus jubch08, purification and characterization of the enzyme and determination of antagonistic activity of the bacterium against fusarium oxysporum. medium supplemented with 0.5 % chitin and peptone, at initial ph 8.0, when incubated at 35 °c for 72 h favored highest chitinase production. the enzyme was purified 25.1-fold to homogeneity. the chitinase was found to be th ... | 2016 | 28330157 |
| sensitive change of iso-branched fatty acid (iso-15:0) in bacillus pumilus pamc 23174 in response to environmental changes. | in this study, the environmental adaptive metabolic processes were investigated using a psychrotrophic polar bacterium bacillus pumilus pamc 23174 in response to various temperatures and nutrients, especially in regard to the synthesis of fatty acids. fatty acid methyl ester analysis was performed using gas chromatography-mass spectrometry and we found that a sensitive changes in iso-branched fatty acid (iso-15:0) synthesis occurred when adjusting the nutritional ratio of branched chain fatty ac ... | 2016 | 26566954 |
| heterologous expression of mannanase and developing a new reporter gene system in lactobacillus casei and escherichia coli. | reporter gene systems are useful for studying bacterial molecular biology, including the regulation of gene expression and the histochemical analysis of protein products. here, two genes, β-1,4-mannanase (manb) from bacillus pumilus and β-glucuronidase (gusa) from escherichia coli k12, were cloned into the expression vector pelx1. the expression patterns of these reporter genes in lactobacillus casei were investigated by measuring their enzymatic activities and estimating their recombinant prote ... | 2015 | 26562012 |
| chitin from penaeus merguiensis via microbial fermentation processing and antioxidant activity. | the ability of three proteases producing bacteria such as pseudomonas aeruginosa, serratia marcescens, and bacillus pumilus on the demineralization and deproteinzation efficiency of shrimp waste, for chitin extraction, was investigated. statistical analysis of data was showed a significant difference between the percentage of demineralization and deproteinization in different bacteria species (p<0.05). the highest deproteinization (74.76%) and demineralization rate (78.46%) was obtained with p. ... | 2016 | 26519877 |
| isolation and characterization of two novel psychrotrophic decabromodiphenyl ether-degrading bacteria from river sediments. | decabromodiphenyl ether (bde-209) is a brominated flame retardant and a priority contaminant. currently, little information is available about its significance in the environment, specifically about its susceptibility to aerobic biotransformation at low temperature. in this work, five phylogenetically diverse bde-209-degrading bacterial strains were isolated from river sediments of northern china. these strains were distributed among four different genera-acinetobacter, pseudomonas, bacillus and ... | 2016 | 26517991 |
| matrix production, pigment synthesis, and sporulation in a marine isolated strain of bacillus pumilus. | the ability to produce an extracellular matrix and form multicellular communities is an adaptive behavior shared by many bacteria. in bacillus subtilis, the model system for spore-forming bacteria, matrix production is one of the possible differentiation pathways that a cell can follow when vegetative growth is no longer feasible. while in b. subtilis the genetic system controlling matrix production has been studied in detail, it is still unclear whether other spore formers utilize similar mecha ... | 2015 | 26506360 |
| studies on properties of the xylan‑binding domain and linker sequence of xylanase xyng1‑1 from paenibacillus campinasensis g1‑1. | xylanase xyng1-1 from paenibacillus campinasensis g1-1 consists of a catalytic domain (cd), a family 6_36 carbohydrate-binding module which is a xylan-binding domain (xbd), and a linker sequence (ls)between them. the structure of xyng1-3 from bacillus pumilus g1-3 consists only of a cd. to investigate the functions and properties of the xbd and ls of xyng1-1, two truncated forms (xyng1-1cdl, xyng1-1cd) and three fusion derivatives (xyng1-3cdl, xyng1-3cdx and xyng1-3cdlx) were constructed and bio ... | 2015 | 26467249 |
| sterilization resistance of bacterial spores explained with water chemistry. | bacterial spores can survive for long periods without nutrients and in harsh environmental conditions. this survival is influenced by the structure of the spore, the presence of protective compounds, and water retention. these compounds, and the physical state of water in particular, allow some species of bacterial spores to survive sterilization schemes with hydrogen peroxide and uv light. the chemical nature of the spore core and its water has been a subject of some contention and the chemical ... | 2015 | 26435315 |
| identification, sequencing and comparative analysis of pbp15.s plasmid from the newly described entomopathogen bacillus pumilus 15.1. | the bacillus pumilus 15.1 strain, a recently described entomopathogenic strain active against ceratitis capitata, contains at least two extrachromosomal elements, pbp15.1s and pbp15.1b. given that b. pumilus is not a typical entomopathogenic bacterium, the acquisition of this extrachromosomal dna may explain why b. pumilus 15.1 is toxic to an insect. one of the plasmids present in the strain, the pbp15.1s plasmid, was sub-cloned, sequenced and analyzed using bioinformatics to identify any potent ... | 2015 | 26416357 |
| study of the radiation effect of 99mo/99mtc generator on bacillus subtilis and bacillus pumilus species. | in this work, molybdenum-99 loaded columns were challenged with bacillus subtilis vegetative cells and bacillus pumilus spores inside and outside the alumina column, and microbial recovery and radiation effect were assessed. alumina was a barrier for the passage of microorganisms regardless the species, whilst spores were more retained than vegetative cells with a lower microbial recovery, without significant differences between 9.25 and 74 gbq generators. bacillus pumilus biological indicator s ... | 2016 | 26408912 |
| draft genome sequence of the entomopathogenic bacterium bacillus pumilus 15.1, a strain highly toxic to the mediterranean fruit fly ceratitis capitata. | we present the draft whole-genome sequence of the entomopathogenic bacillus pumilus 15.1 strain that consists of 3,795,691 bp and 3,776 predicted protein-coding genes. this genome sequence provides the basis for understanding the potential mechanism behind the toxicity and virulence of b. pumilus 15.1 against the mediterranean fruit fly. | 2015 | 26404596 |
| construction of novel shuttle expression vectors for gene expression in bacillus subtilis and bacillus pumilus. | a native plasmid (psu01) was detected by genome sequencing of bacillus subtilis strain s1-4. two psu01-based shuttle expression vectors psu02-ap and psu03-ap were constructed enabling stable replication in b. subtilis wb600. these vectors contained the reporter gene apre, encoding an alkaline protease from bacillus pumilus ba06. the expression vector psu03-ap only possessed the minimal replication elements (rep, sso, dso) and exhibited more stability on structure, suggesting that the rest of the ... | 2015 | 26377132 |
| sugarcane bagasse as support for immobilization of bacillus pumilus hz-2 and its use in bioremediation of mesotrione-contaminated soils. | the degrading microorganisms isolated from environment usually fail to degrade pollutants when used for bioremediation of contaminated soils; thus, additional treatments are needed to enhance biodegradation. in the present study, the potential of sugarcane bagasse as bacteria-immobilizing support was investigated in mesotrione biodegradation. a novel isolate bacillus pumilus hz-2 was applied in bacterial immobilization, which was capable of degrading over 95 % of mesotrione at initial concentrat ... | 2015 | 26337896 |
| draft genome sequence of the shellfish larval probiotic bacillus pumilus ri06-95. | bacillus pumilus ri06-95 is a marine bacterium isolated in narragansett, rhode island, which has shown probiotic activity against marine pathogens in larval shellfish. we report the genome of b. pumilus ri06-95, which provides insight into the microbe's probiotic ability and may be used in future studies of the probiotic mechanism. | 2015 | 26337873 |
| identification of oxidoreductases from the petroleum bacillus safensis strain. | a gram-positive bacterium, denominated cfa-06, was isolated from brazilian petroleum in the campos basin and is responsible for the degradation of aromatic compounds and petroleum aromatic fractions. the cfa-06 strain was identified as bacillus safensis using the 16s rrna and gyrase b sequence. enzymatic assays revealed the presence of two oxidoreductases: a catalase and a new oxidoreductase. the oxidoreductases were enzymatically digested and analyzed via esi-ltq-orbitrap mass spectrometry. the ... | 2015 | 28352585 |
| cyanide hydratases and cyanide dihydratases: emerging tools in the biodegradation and biodetection of cyanide. | the purpose of this study is to summarize the current knowledge of the enzymes which are involved in the hydrolysis of cyanide, i.e., cyanide hydratases (chts; ec 4.2.1.66) and cyanide dihydratases (cynd; ec 3.5.5.1). chts are probably exclusively produced by filamentous fungi and widely occur in these organisms; in contrast, cynds were only found in a few bacterial genera. chts differ from cynds in their reaction products (formamide vs. formic acid and ammonia, respectively). several chts were ... | 2015 | 26329848 |
| microbial selenium nanoparticles (senps) and their application as a sensitive hydrogen peroxide biosensor. | the cell-free extract, a crude enzyme (cytosolic and membrane fraction) obtained from an environmental isolate, bacillus pumilus sp. bab-3706 worked as excellent in reducing as well as stabilizing agent and facilitated the formation of stable colloidal selenium nanoparticles (senps). resulting nanoparticles were characterized using uv-vis spectrophotometer, tem, edax, ft-ir and xrd, respectively. a working electrode was modified by coating the surface of indium tin oxide (ito) with colloidal sen ... | 2015 | 26319569 |
| a novel thermostable gh5_7 β-mannanase from bacillus pumilus gbsw19 and its application in manno-oligosaccharides (mos) production. | a novel thermostable mannanase from a newly isolated bacillus pumilus gbsw19 has been identified, expressed, purified and characterized. the enzyme shows a structure comprising a 28 amino acid signal peptide, a glycoside hydrolase family 5 (gh5) catalytic domain and no carbohydrate-binding module. the recombinant mannanase has molecular weight of 45 kda with an optimal ph around 6.5 and is stable in the range from ph 5-11. meanwhile, the optimal temperature is around 65 °c, and it retains 50% re ... | 2015 | 26215338 |
| effects of direct-fed bacillus pumilus 8g-134 on feed intake, milk yield, milk composition, feed conversion, and health condition of pre- and postpartum holstein cows. | the usage of direct-fed microbials (dfm) has become common in the dairy industry, but questions regarding choice of strain, mode of action, and efficacy remain prevalent. the objective of this study was to evaluate the effects of a dfm (bacillus pumilus 8g-134) on pre- and postpartum performance and incidence of subclinical ketosis in early lactation. forty-three multiparous holstein cows were assigned to 2 treatments in a randomized complete block design; cows in the direct-fed microbial treatm ... | 2015 | 26162791 |
| production of a cellulase-free alkaline xylanase from bacillus pumilus mtcc 5015 by submerged fermentation and its application in biobleaching. | here, we described the production of a cellulase-free alkaline xylanase from bacillus pumilus mtcc 5015 by submerged fermentation and its application in biobleaching. various process parameters affecting xylanase production by b. pumilus were optimized by adopting a plackett-burman design (pbd) as well as response surface methodology (rsm). these statistical methods aid in improving the enzyme yield by analysing the individual crucial components of the medium. maximum production was obtained wit ... | 2015 | 26155675 |
| synthesis and characterization of magnetite/silver/antibiotic nanocomposites for targeted antimicrobial therapy. | the article is devoted to preparation and characterization of magnetite/silver/antibiotic nanocomposites for targeted antimicrobial therapy. magnetite nanopowder was produced by thermochemical technique; silver was deposited on the magnetite nanoparticles in the form of silver clusters. magnetite/silver nanocomposite was investigated by xrd, sem, tem, afm, xps, edx techniques. adsorptivity of magnetite/silver nanocomposite towards seven antibiotics from five different groups was investigated. it ... | 2015 | 26117765 |
| [identification of 2',3'-cgmp as an intermediate of rna catalytic cleavage by binase and evaluation of its biological action]. | binase--bacillus pumilus rnase--endonuclease cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-oh residue of adjacent nucleotides with the formation of corresponding intermediate 2',3'-cgmp. subsequent hydrolysis of 2',3'-cgmp into 3'-phosphate is highly specific and occurs slowly so the question arises about the existing time of that positional isomer during rna catalytic cleavage by binase and about 2',3'-cgmp role in antitumor activity of the enzyme: in present work b ... | 2015 | 26050470 |
| genomic analysis of a ginger pathogen bacillus pumilus providing the understanding to the pathogenesis and the novel control strategy. | bacillus pumilus has been widely identified as a pathogen of plant and human, while the genetic information is rarely available for pathogenic b. pumilus strains. b. pumilus gr8 is a pathogen that causes ginger rhizome rot disease by invading ginger rhizome parenchymatous tissues, growing in the extracellular space, and producing plant cell wall-degrading enzymes to destroy ginger cells. in this study, the genome of gr8 was sequenced and characterized. this genome was the third completely sequen ... | 2015 | 25989507 |
| salt stress represses production of extracellular proteases in bacillus pumilus. | bacillus pumilus is able to secrete subtilisin-like prote-ases, one of which has been purified and characterized biochemically, demonstrating great potential for use in industrial applications. in the current study, the biosynthesis and transcription of extracellular pro-teases in b. pumilus (ba06) under salt stress were investigated using various methods, including a proteolytic assay, zymogram analysis, and real-time pcr. our results showed that total extracellular proteolytic activity, both i ... | 2015 | 25966269 |
| complete genome sequence of bacillus pumilus w3: a strain exhibiting high laccase activity. | here we report the full genome sequence of bacillus pumilus w3, which was isolated from raw gallnut honey in nandan county, guangxi province of china, showing high cota-laccase activity. the w3 strain contains 3,745,123bp with gc content of 41.39%, and contains 3695 protein-coding genes, 21 rrnas and 70 trnas. | 2015 | 25957807 |
| sterilization of hydrogen peroxide resistant bacterial spores with stabilized chlorine dioxide. | bacillus pumilus safr-032 spores isolated from a clean room environment are known to exhibit enhanced resistance to peroxide, desiccation, uv radiation and chemical disinfection than other spore-forming bacteria. the survival of b. pumilus safr-032 spores to standard clean room sterilization practices requires development of more stringent disinfection agents. here, we report the effects of a stabilized chlorine dioxide-based biocidal agent against spores of b. pumilus safr-032 and bacillus subt ... | 2015 | 25897406 |
| [expression optimization of bacillus pumilus subtilisin-like proteinase]. | the characteristics of bacillus pumilus subtilisin-like protease expression were investigated. potential binding sites for the transcription factor degu~p have been identified in the regulatory region of the enzyme gene, which have been optimized for interaction with the regulatory protein. the expression of the extracellular subtilisin-like protease modified gene has been examined. it was established that the modification of one of the sites has resulted in increased expression of the proteinas ... | 2015 | 25895372 |
| efficient secretory production of cota-laccase and its application in the decolorization and detoxification of industrial textile wastewater. | fungal laccases are typically unstable at high ph and temperature conditions, which limit their application in the decolorization of textile wastewater. by contrast, the highly stable bacterial laccases can function within a wider ph range and at high temperatures, thus have significant potential in treatment for textile wastewater. in our previous work, a thermo-alkali-stable cota-laccase gene was cloned from bacillus pumilus w3 and overexpressed in escherichia coli. in this study, the robust c ... | 2015 | 25847445 |
| growth and (137)cs uptake of four brassica species influenced by inoculation with a plant growth-promoting rhizobacterium bacillus pumilus in three contaminated farmlands in fukushima prefecture, japan. | the effectiveness of the plant growth-promoting rhizobacterium bacillus pumilus regarding growth promotion and radiocesium ((137)cs) uptake was evaluated in four brassica species grown on different (137)cs contaminated farmlands at fukushima prefecture in japan from june to august 2012. b. pumilus inoculation did not enhance growth in any of the plants, although it resulted in a significant increase of (137)cs concentration and higher (137)cs transfer from the soil to plants. the brassica specie ... | 2015 | 25847170 |
| a membraneless air-breathing hydrogen biofuel cell based on direct wiring of thermostable enzymes on carbon nanotube electrodes. | a biocathode was designed by the modification of a carbon nanotube (cnt) gas-diffusion electrode with bilirubin oxidase from bacillus pumilus, achieving high current densities up to 3 ma cm(-2) for the reduction of o2 from air. a membraneless air-breathing hydrogen biofuel cell was designed by combination of this cathode with a functionalized cnt-based hydrogenase anode. | 2015 | 25845356 |
| the hyperaccumulator sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil. | endophyte-assisted phytoremediation has recently been suggested as a successful approach for ecological restoration of metal contaminated soils, however little information is available on the influence of endophytic bacteria on the phytoextraction capacity of metal hyperaccumulating plants in multi-metal polluted soils. the aims of our study were to isolate and characterize metal-resistant and 1-aminocyclopropane-1-carboxylate (acc) utilizing endophytic bacteria from tissues of the newly discove ... | 2015 | 25796039 |
| draft genome sequences of five spore-forming food isolates of bacillus pumilus. | here, we report the draft genome sequences of five food isolates of bacillus pumilus, a spore-forming gram-positive bacterium. | 2015 | 25767247 |
| a novel intracellular nitrogen-fixing symbiosis made by ustilago maydis and bacillus spp. | we observed that the maize pathogenic fungus ustilago maydis grew in nitrogen (n)-free media at a rate similar to that observed in media containing ammonium nitrate, suggesting that it was able to fix atmospheric n2 . because only prokaryotic organisms have the capacity to reduce n2 , we entertained the possibility that u. maydis was associated with an intracellular bacterium. the presence of nitrogenase in the fungus was analyzed by acetylene reduction, and capacity to fix n2 by use of (15) n2 ... | 2015 | 25754368 |
| microbiological bioassay using bacillus pumilus to detect tetracycline in milk. | the tetracyclines (tcs) are widely used in the treatment of several diseases of cattle and their residues may be present in milk. to control these residues it is necessary to have available inexpensive screening methods, user-friendly and capable of analysing a high number of samples. the purpose of this study was to design a bioassay of microbiological inhibition in microtiter plates with spores of bacillus pumilus to detect tcs at concentrations corresponding to the maximum residue limits (mrl ... | 2015 | 25720802 |
| isolation and identification of local bacillus isolates for xylanase biosynthesis. | bacillus species are attractive industrial organisms due to their rapid growth rates leading to a short fermentation cycle and for their capacity to secrete important enzymes and proteins such as xylanase into the extracellular medium. considering the industrial importance of xylanase, in this current study, bacillus spp. were isolated from different soils and were screened for their xylanase production. | 2014 | 25705364 |
| antibacterial metabolites and bacteriolytic enzymes produced by bacillus pumilus during bacteriolysis of arthrobacter citreus. | the marine isolate bacillus pumilus sbug 1800 is able to lyse living cells of arthrobacter citreus on solid media as well as pasteurized a. citreus cells in liquid mineral salt medium. the cultivation of b. pumilus in the presence of pasteurized a. citreus is accompanied by an enhanced production of 2,5-diketopiperazines (dkps). dkps inhibit bacterial growth, but do not seem to cause bacteriolysis. this study shows that b. pumilus also lyses living cells of a. citreus in co-culture experiments a ... | 2015 | 25678259 |
| unprecedented access of phenolic substrates to the heme active site of a catalase: substrate binding and peroxidase-like reactivity of bacillus pumilus catalase monitored by x-ray crystallography and epr spectroscopy. | heme-containing catalases and catalase-peroxidases catalyze the dismutation of hydrogen peroxide as their predominant catalytic activity, but in addition, individual enzymes support low levels of peroxidase and oxidase activities, produce superoxide, and activate isoniazid as an antitubercular drug. the recent report of a heme enzyme with catalase, peroxidase and penicillin oxidase activities in bacillus pumilus and its categorization as an unusual catalase-peroxidase led us to investigate the e ... | 2015 | 25663126 |
| complete genome sequence of bacillus pumilus strain wp8, an efficient plant growth-promoting rhizobacterium. | bacillus pumilus strain wp8 is an efficient plant growth-promoting rhizobacterium. here, we present the complete genome of wp8 and its genes involved in plant growth promotion and biocontrol. | 2015 | 25614565 |
| c-terminal hybrid mutant of bacillus pumilus cyanide dihydratase dramatically enhances thermal stability and ph tolerance by reinforcing oligomerization. | to investigate the impact of the highly variable c-terminal domain of cyanide dihydratase, a member of the nitrilase superfamily, on its activity and stability. | 2015 | 25597384 |
| genomic and chemical insights into biosurfactant production by the mangrove-derived strain bacillus safensis ccma-560. | many bacillus species can produce biosurfactant, although most of the studies on lipopeptide production by this genus have been focused on bacillus subtilis. surfactants are broadly used in pharmaceutical, food and petroleum industry, and biological surfactant shows some advantages over the chemical surfactants, such as less toxicity, production from renewable, cheaper feedstocks and development of novel recombinant hyperproducer strains. this study is aimed to unveil the biosurfactant metabolic ... | 2015 | 25586584 |
| increased mrna expression of selected pro-inflammatory factors in inflamed bovine endometrium in vivo as well as in endometrial epithelial cells exposed to bacillus pumilus in vitro. | endometrial epithelium plays a crucial role in the first immune response to invading bacteria by producing cytokines and chemokines. the aim of this study was to investigate the first inflammatory response of the endometrium in vivo and in vitro. gene expression of several pro-inflammatory factors and toll-like receptors (tlr2, -4, -6) was determined in endometrial cytobrush samples obtained from healthy cows and cows with clinical or subclinical endometritis. endometrial epithelial cells were c ... | 2015 | 25562589 |
| new insight into secreted ribonuclease structure: binase is a natural dimer. | the biological effects of ribonucleases (rnases), such as the control of the blood vessels growth, the toxicity towards tumour cells and antiviral activity, require a detailed explanation. one of the most intriguing properties of rnases which can contribute to their biological effects is the ability to form dimers, which facilitates efficient rna hydrolysis and the evasion of ribonuclease inhibitor. dimeric forms of microbial rnase binase secreted by bacillus pumilus (former b. intermedius) have ... | 2014 | 25551440 |
| probing c-terminal interactions of the pseudomonas stutzeri cyanide-degrading cynd protein. | the cyanide dihydratases from bacillus pumilus and pseudomonas stutzeri share high amino acid sequence similarity throughout except for their highly divergent c-termini. however, deletion or exchange of the c-termini had different effects upon each enzyme. here we extended previous studies and investigated how the c-terminus affects the activity and stability of three nitrilases, the cyanide dihydratases from b. pumilus (cyndpum) and p. stutzeri (cyndstut) and the cyanide hydratase from neurospo ... | 2015 | 25549622 |
| halotolerant pgprs prevent major shifts in indigenous microbial community structure under salinity stress. | the resilience of soil microbial populations and processes to environmental perturbation is of increasing interest as alteration in rhizosphere microbial community dynamics impacts the combined functions of plant-microbe interactions. the present study was conducted to investigate the effect of inoculation with halotolerant rhizobacteria bacillus pumilus (str2), halomonas desiderata (str8), and exiguobacterium oxidotolerans (str36) on the indigenous root-associated microbial (bacterial and funga ... | 2015 | 25542205 |
| single amino acid mutation alters thermostability of the alkaline protease from bacillus pumilus: thermodynamics and temperature dependence. | dehairing alkaline protease (dhap) from bacillus pumilus ba06 has been demonstrated to have high catalytic efficiency and good thermostability, with potential application in leather processing. in order to get insights into its catalytic mechanism, two mutants with single amino acid substitution according to the homology modeling and multiple sequence alignment were characterized in thermodynamics of thermal denaturation and temperature dependence of substrate hydrolysis. the results showed that ... | 2015 | 25534779 |
| [effect of bacillus pumilus ribonuclease on the paramagnetic centers of microbial cells]. | potential clinical application of bacillus pumulus cytotoxic ribonuclease (binase) selectively inducing the death of tumor cells makes it imperative to investigate its effect on the normal human microflora. flow cytometry was used to determine that binase concentration causing the apoptosis of cancer cells had no effect of the viability of escherichia coli k12. the changes in the paramagnetic centers of e. coli k12 cells in the presence of nontoxic binase concentrations revealed by epr spectrosc ... | 2014 | 25507458 |
| action spectra for validation of pathogen disinfection in medium-pressure ultraviolet (uv) systems. | ultraviolet (uv) reactors used for disinfecting water and wastewater must be validated and monitored over time. the validation process requires understanding the photochemical properties of the pathogens of concern and the challenge microorganisms used to represent them. specifically for polychromatic uv systems, the organisms' dose responses to uv light and their sensitivity across the uv spectrum must be known. this research measured the uv spectral sensitivity, called action spectra, of crypt ... | 2015 | 25506761 |
| diversity and health status specific fluctuations of intrauterine microbial communities in postpartum dairy cows. | for the interpretation of clinical findings of endometritis and the development of disease prevention and intervention strategies a better understanding of the dynamics and interactions within intrauterine bacterial communities in healthy and diseased cows is required. to gain deeper insights into fluctuations within the uterine microbiota, intrauterine samples were collected from 122 cows at the day of calving, days 3, 9, 15, 21 and 28 postpartum. a total of 2052 bacterial isolates were identif ... | 2015 | 25497238 |
| treatment of textile effluent in a developed phytoreactor with immobilized bacterial augmentation and subsequent toxicity studies on etheostoma olmstedi fish. | a static hydroponic bioreactor using nursery grown plants of pogonatherum crinitum along with immobilized bacillus pumilus cells was developed for the treatment of textile wastewater. independent reactors with plants and immobilized cells were also kept for performance and efficacy evaluation. the effluent samples characterized before and after their treatment showed that the plant-bacterial consortium reactor was more efficient than those of individual plant and bacterium reactors. cod, bod, ad ... | 2015 | 25464312 |
| binding characteristics and synergistic effects of bacterial expansins on cellulosic and hemicellulosic substrates. | expansins are non-catalytic proteins which loosen plant cell wall structure. in this study, binding kinetics and synergistic action of five bacterial expansins on cellulosic and hemicellulosic polysaccharides were studied. the expansins differed in binding capacity (bmax) and affinity (kd) for different substrates. a common pattern of binding efficiency (bmax/kd) was found among the expansins tested, in which efficiency was greatest for the phosphoric acid-swollen cellulose (pasc), then the hemi ... | 2015 | 25460993 |
| [effect of bacillus pumilus ribonuclease on the paramagnetic centers of microbial cells]. | potential clinical application of bacillus pumulus cytotoxic ribonuclease (binase) selectively inducing the death of tumor cells makes it imperative to investigate its effect on the normal human microflora. flow cytometry was used to determine that binase concentration causing the apoptosis of cancer cells had no effect of the viability of escherichia coli k12. the changes in the paramagnetic centers of e. coli k12 cells in the presence of nontoxic binase concentrations revealed by epr spectrosc ... | 2014 | 25423735 |
| effects of actin-like proteins encoded by two bacillus pumilus phages on unstable lysogeny, revealed by genomic analysis. | we characterized two newly isolated myoviruses, bp8p-c and bp8p-t, infecting the ginger rhizome rot disease pathogen bacillus pumilus gr8. the plaque of bp8p-t exhibited a clear center with a turbid rim, suggesting that bp8p-t could transform into latent phage. lysogeny assays showed that both the two phages could form latent states, while bp8p-t could form latent phage at a higher frequency and stability than bp8p-c. the genomes of bp8p-c and bp8p-t were 151,417 and 151,419 bp, respectively; bo ... | 2015 | 25344242 |
| purification and characterization of a fibrinolytic enzyme from bacillus pumilus 2.g isolated from gembus, an indonesian fermented food. | bacillus pumilus 2.g isolated from gembus, an indonesian fermented soybean cake, secretes several proteases that have strong fibrinolytic activities. a fibrinolytic enzyme with an apparent molecular weight of 20 kda was purified from the culture supernatant of b. pumilus 2.g by sequential application of ammonium sulfate precipitation, ion-exchange chromatography, and hydrophobic chromatography. the partially purified enzyme was stable between ph 5 and ph 9 and temperature of less than 60°c. fibr ... | 2014 | 25320719 |
| differentiation of bacillus pumilus and bacillus safensis using maldi-tof-ms. | matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof-ms) despite being increasingly used as a method for microbial identification, still present limitations in which concerns the differentiation of closely related species. bacillus pumillus and bacillus safensis, are species of biotechnological and pharmaceutical significance, difficult to differentiate by conventional methodologies. in this study, using a well-characterized collection of b. pumillus and b. saf ... | 2014 | 25314655 |
| improving the thermoactivity and thermostability of pectate lyase from bacillus pumilus for ramie degumming. | thermostable alkaline pectate lyases can be potentially used for enzymatically degumming ramie in an environmentally sustainable manner and as an alternative to the currently used chemical-based ramie degumming processes. to assess its potential applications, pectate lyase from bacillus pumilus (atcc 7061) was cloned and expressed in escherichia coli. evolutionary strategies were applied to generate efficient ramie degumming enzymes. obtained from site-saturation mutagenesis and random mutagenes ... | 2015 | 25287558 |
| cell physiology of the biotechnological relevant bacterium bacillus pumilus-an omics-based approach. | members of the species bacillus pumilus get more and more in focus of the biotechnological industry as potential new production strains. based on exoproteome analysis, b. pumilus strain jo2, possessing a high secretion capability, was chosen for an omics-based investigation. the proteome and metabolome of b. pumilus cells growing either in minimal or complex medium was analyzed. in total, 1542 proteins were identified in growing b. pumilus cells, among them 1182 cytosolic proteins, 297 membrane ... | 2014 | 25281541 |
| probiotic bacillus pumilus se5 shapes the intestinal microbiota and mucosal immunity in grouper epinephelus coioides. | the health benefits of probiotics are thought to occur, at least in part, through an improved intestinal microbial balance in fish, although the molecular mechanisms whereby probiotics modulate the intestinal microbiota by means of activation of mucosal immunity are rarely explored. in this study, the effects of viable and heat-inactivated probiotic bacillus pumilus se5 on the intestinal dominant microbial community and mucosal immune gene expression were evaluated. the fish were fed for 60 d wi ... | 2014 | 25266899 |
| probing the crucial role of leu31 and thr33 of the bacillus pumilus cbs alkaline protease in substrate recognition and enzymatic depilation of animal hide. | the sapb gene, encoding bacillus pumilus cbs protease, and seven mutated genes (sapb-l31i, sapb-t33s, sapb-n99y, sapb-l31i/t33s, sapb-l31i/n99y, sapb-t33s/n99y, and sapb-l31i/t33s/n99y) were overexpressed in protease-deficient bacillus subtilis db430 and purified to homogeneity. sapb-n99y and rsapb displayed the highest levels of keratinolytic activity, hydrolysis efficiency, and enzymatic depilation. interestingly, and at the semi-industrial scale, rsapb efficiently removed the hair of goat hid ... | 2014 | 25264614 |
| bacterial enzymes effectively digest alzheimer's β-amyloid peptide. | aggregated β-amyloid peptides play key roles in the development of alzheimer's disease, and recent evidence suggests that microbial particles, among others, can facilitate their polymerization. bacterial enzymes, however, have been proved to be beneficial in degrading pathological fibrillar structures in clinical settings, such as strepto-kinases in resolving blood-clots. the purpose of this study was to investigate the ability of bacterial substances to effectively hydrolyze β-amyloid peptides. ... | 2014 | 25262575 |
| molecular characterization of a proteolysis-resistant lipase from bacillus pumilus sg2. | proteolysis-resistant lipases can be well exploited by industrial processes which employ both lipase and protease as biocatalysts. a proteolysis resistant lipase from bacillus pumilus sg2 was isolated, purified and characterized earlier. the lipase was resistant to native and commercial proteases. in the present work, we have characterized the lip gene which encodes the proteolysis-resistant lipase from bacillus pumilus sg2. the parameters and structural details of lipase were analysed. the lip ... | 2014 | 25242920 |
| immobilization of xylanase purified from bacillus pumilus vlk-1 and its application in enrichment of orange and grape juices. | this study was conducted to evaluate the efficacy of purified free and immobilized xylanase in enrichment of fruit juices. extracellular xylanase produced from bacillus pumilus vlk-1 was purified to apparent homogeneity by 15.4-fold with 88.3 % recovery in a single step using cm-sephadex c-50. purified xylanase showed a single band on sds-polyacrylamide gel with a molecular mass of 22.0 kda. the purified enzyme was immobilized on glutaraldehyde-activated aluminum oxide pellets and the immobiliza ... | 2014 | 25190829 |
| vulnerability of bacillus spores and of related genera to physical impaction injury with particular reference to spread-plating. | to examine whether bacterial spores are vulnerable to impaction injury during standard spread-plating or to other modes of physical impaction. | 2014 | 25073977 |