Publications

heterologous expression of the bacillus pumilus endo-beta-xylanase (xyna) gene in the yeast saccharomyces cerevisiae.	the endo-beta-xylanase-encoding gene (xyna) of bacillus pumilus pls was isolated from a genomic dna library and the open reading frame (orf) was inserted in expression vectors for the yeast saccharomyces cerevisiae. plasmid pfn3 harboured the xyna orf fused to the yeast mating pheromone alpha-factor signal sequence (mfalpha1s) under the control of the alcohol dehydrogenase ii gene promotor (adh2p) and terminator (adh2t) sequences. in plasmid pfn4, the mfalpha1s-xyna orf was brought under the con ...	2001	11549015
purification, crystallization and preliminary x-ray analysis of an acetylxylan esterase from bacillus pumilus.	the gene encoding for acetylxylan esterase from bacillus pumilus has been cloned and expressed in escherichia coli. the recombinant protein has been purified to homogeneity and crystallized. the crystals obtained are of regular shape of dimensions 0.05 x 0.05 x 0.05 mm with r32 symmetry and diffract to 2.0 a using synchrotron radiation.	2001	11717513
isolation and characterization of a cell-associated protein of bacillus pumilus ph-01.	a cell-associated protein released from bacillus pumilus ph-01 showed an affinity for some dioxins, like 1,2,3,4-tetrachlorodibenzo-p-dioxin (tcdd) and 1,2,3,4-tetrachlorodibenzofuran (tcdf), and the concentration of the protein increased when b. pumilus ph-01 was boiled in minimal salts medium. sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-mass spectrometry revealed that the boiled culture supernatant contained a major protein with a m ...	2001	11549009
consumption of tropospheric levels of methyl bromide by c(1) compound-utilizing bacteria and comparison to saturation kinetics.	pure cultures of methylotrophs and methanotrophs are known to oxidize methyl bromide (mebr); however, their ability to oxidize tropospheric concentrations (parts per trillion by volume [pptv]) has not been tested. methylotrophs and methanotrophs were able to consume mebr provided at levels that mimicked the tropospheric mixing ratio of mebr (12 pptv) at equilibrium with surface waters ( approximately 2 pm). kinetic investigations using picomolar concentrations of mebr in a continuously stirred t ...	2001	11722890
characterization of plant growth promoting rhizobacteria isolated from polluted soils and containing 1-aminocyclopropane-1-carboxylate deaminase.	fifteen bacterial strains containing 1-aminocyclopropane-1-carboxylate (acc) deaminase were isolated from the rhizoplane of pea (pisum sativum l.) and indian mustard (brassica juncea l.) grown in different soils and a long-standing sewage sludge contaminated with heavy metals. the isolated strains were characterized and assigned to various genera and species, such as pseudomonas brassicacearum, pseudomonas marginalis, pseudomonas oryzihabitans, pseudomonas putida, pseudomonas sp., alcaligenes xy ...	2001	11547884
fermentation of starch for enhanced alkaline protease production by constructing an alkalophilic bacillus pumilus strain.	a new engineering strain, bacillus pumilus c172-14 (pbx 96), was obtained by introducing the pbx 96 plasmid, which carries the alpha-amylase amy gene, into the host strain of alkalophilic bacillus pumilus c172 via transformation. the newly constructed strain was found to express the amy gene and could use starch instead of glucose or starch hydrolysate as carbon source for its fermentation of alkaline protease. the pbx 96 plasmid in the new host was found to be segregationally and structurally s ...	2001	11693913
bacterial populations associated with rice seed in the tropical environment.	abstract during the 1995 wet season, harvested rice seed was collected from farmers' fields at different locations in iloilo, philippines. bacterial isolations from crushed seed yielded 428 isolates. the isolates were characterized by box-polymerase chain reaction fingerprinting of total genomic dna and represented 151 fingerprint types (fpt). most fpts were found on a single occasion, although matching fingerprints for isolates from different samples also were found. identifications were made b ...	2001	18943348
[cloning and dna sequencing of bacillus pumilus endo-1,4-beta-glucanase gene].	bacillus pumilus s-27 screened from marine environment produces an extracellular endo-1,4-beta-glucanase. the enzyme has its optimal activity at ph 5.0-7.0 and 55 degrees c and still retained 79.0% of it at ph 9.0. the gene encoded this endo-1,4-beta-glucanase was cloned and sequenced. the structural gene contained an open reading frame of 1980 bp, corresponding to 660 amino acids, the amino acid sequence of this enzyme is very close to that of an eg of c. cellulovorans and an eg of bacillus sp. ...	2001	12549193
effects of inoculation with pgpr bacillus and pisolithus tinctorius on pinus pinea l. growth, bacterial rhizosphere colonization, and mycorrhizal infection.	the effect of co-inoculation with pisolithus tinctorius and a pgpr belonging to the genus bacillus (bacillus licheniformis cect 5106 and bacillus pumilus cect 5105) in enhancing growth of pinus pinea plants and the changes that occurred in rhizosphere microbial communities and the degree of mycorrhization were evaluated. both bacterial strains of bacillus promote the growth of pinus pinea seedlings, but this biological effect does not imply a synergic effect with mycorrhizal infection. however, ...	2001	12032619
purification and some properties of a novel chitosanase from bacillus subtilis kh1.	one of at least two chitosanases secreted in the culture filtrate of bacillus subtilis kh1 was purified by two sequential deae sepharose cl-6b chromatographies, followed by sephacryl s-100 hr gel chromatography. the purified enzyme was homogenous as judged by sds-page. it showed an estimated molecular weight and pi of 28,000 and 8.3, respectively. the enzyme drastically reduced the viscosity of highly deacetylated chitosan substrates, with the subsequent formation of chitooligosaccharides [(glcn ...	2000	12483600
the trap-like spla protein is a trans-acting negative regulator of spore photoproduct lyase synthesis during bacillus subtilis sporulation.	uv resistance of bacterial endospores derives from a unique dna photochemistry in which the major uv photoproduct is the thymine dimer 5-thyminyl-5,6-dihydrothymine (spore photoproduct [sp]) instead of cyclobutane pyrimidine dimers. repair of sp during spore germination is due in large part to the activity of the enzyme sp lyase encoded by splb, the second cistron of the splab operon. expression of the splab operon in bacillus subtilis is transcriptionally activated by the esigma(g) form of rna ...	2000	10629212
soybean-milk-coagulating activity of bacillus pumilus derives from a serine proteinase.	a proteolytic enzyme from bacillus pumilus strain tyo-67, which was able to coagulate the protein in soybean milk, was characterized enzymologically. the optimum ph and temperature for its activities were 9.0 and 50 degrees c, respectively. the enzyme was strongly believed to be a serine proteinase because it was completely inhibited by the addition of diisopropyl fluorophosphate or phenylmethanesulfonyl fluoride. hammerstein milk casein, cytochrome c and soybean protein were good substrates for ...	2000	10803893
the acetyl xylan esterase of bacillus pumilus belongs to a family of esterases with broad substrate specificity.	the bacillus pumilus gene encoding acetyl xylan esterase (axe) was identified and characterized. the axe gene was expressed and the recombinant enzyme produced in escherichia coli was purified and characterized. the recombinant enzyme displayed similar properties to the acetyl xylan esterase (axe) purified from b. pumilus. the axe primary structure was 76% identical to the cephalosporin c deacetylase of b. subtilis, and 40% to two recently identified axes from thermoanaerobacterium and thermotog ...	2000	10878123
sequence of the gene encoding an alkaline serine proteinase of bacillus pumilus tyo-67.	the complete nucleotide sequence of the gene encoding an alkaline serine proteinase (aprp) of bacillus pumilus tyo-67 was determined. the sequence analysis showed an open reading frame of 1,149 bp (383 amino acids) that encoded a signal peptide consisting of 29 residues and a propeptide of 79 residues. the deduced 3 amino acid residues, d32, h64, and s221, were identical with 3 essential amino acids in the catalytic center of subtilases. the sequence around these residues revealed that aprp was ...	2000	10888358
a new factor from bacillus mesentericus which promotes the growth of bifidobacterium.	it was reported previously that supernatants of cultures of bacillus mesentericus to-a promote the growth of bifidobacterium species. in this study, a new growth-promoting factor, bm-1, was purified from the supernatant of such a culture and its chemical structure was determined. bm-1 was identified as 3,3-dihydroxyazetidine, and it promoted the growth of several strains of bifidobacterium.	2000	10738983
extraction in aqueous two-phase systems of alkaline xylanase produced by bacillus pumilus and its application in kraft pulp bleaching.	the aim of this work was to extract and to purify xylanase, produced by bacillus pumilus from the crude fermentation broth, using aqueous two-phase systems (atps). the xylanase was extracted by partitioning in atps composed of phosphate and polyethylene glycol (peg). the effect of tie-line length, peg molecular mass and nacl concentrations upon the purification factors and yields of xylanase were investigated by statistical design. the best system studied was that containing 22% peg6000, 10% k2h ...	2000	10942305
antibacterial activity of water and acetone extracts of the roots of euclea natalensis.	water and acetone extracts of the roots of euclea natalensis a.dc. were investigated for their in vitro antibacterial properties. the gram-positive bacteria tested appeared to be more susceptible to the extracts than the gram-negative bacteria. the water and acetone extracts inhibited the growth of bacillus cereus, bacillus pumilus, bacillus subtilis, micrococcus kristinae and staphylococcus aureus at concentrations ranging between 0.1 and 6.0 mg/ml. the water extract did not exert any inhibitor ...	2000	10967488
coexpression of the bacillus pumilus beta-xylosidase (xynb) gene with the trichoderma reesei beta xylanase 2 (xyn2) gene in the yeast saccharomyces cerevisiae.	the xynb gene encoding the bacillus pumilus beta-xylosidase was expressed separately and jointly with the trichoderma reesei beta-xylanase (xyn2) gene in the yeast saccharomyces cerevisiae. both genes were placed under the transcriptional control of the glucose-derepressible alcohol dehydrogenase 2 promoter (adh2p) and terminator (adh2t) sequences. the xynb gene was fused in frame to the yeast mating factor alpha1 secretion sequence (mfalpha1s) to effect secretion in s. cerevisiae. the fusion pr ...	2000	10968632
the dppa gene of bacillus subtilis encodes a new d-aminopeptidase.	different strains of bacillus were screened for their ability to hydrolyse d-alanyl-p-nitroanilide. activity was detected in bacillus pumilus, bacillus brevis, bacillus licheniformis 749i and bacillus subtilis 168. the last strain was the best producer and was selected for the production and purification of the enzyme. the determination of the n-terminal sequence identified the enzyme as the product of the dppa gene (previously named dciaa) belonging to the dipeptide abc transport (dpp) operon e ...	2000	11069674
[studies on submerged fermentation of alkaline beta-1,4-glycanases by bacillus pumilus a-30].	the effects of stirring rate, ph control and feed time of (nh4)2so4 on beta-1,4-glycanases were investigated. the operating conditions of batch fermentation and fed-batch culture were optimized. the results showed there were a large amount of bacterial cells adsorbing on the surface of wheat bran, and stirring rate had a pronounced influence on the adsorption and the production of beta-1,4-glycanases. the uncontrolled ph could promote the induction of beta-1,4-glycanases. when (nh4)2so4 was fed ...	2000	11051825
antimicrobial potency of the leaf - stalk extract of curcuma longa (linn).	the methanolic extract of the leaf-stalk of curcuma longa linn, was tested for its minimum inhibitor concentration (mic) against gram positive-staphylococcus aureus, bacillus pumilus, bacillus subtilis, klebsiella pnemoniae, bacillus cereus, streptococcus pneumoniae, lactobacillus arabinosus and gram negative e.coli, shigella dysenteriae, shigella sonnei, shigella boydii, salmonella typhimurium, proteus mirabilis, and vibrio cholerae strains, further, the ones of inhibition produced by the crude ...	2000	22557005
spatio-temporal distribution of naturally occurring bacillus spp. and other bacteria on the phylloplane of soybean under field conditions.	bacterial populations were surveyed on phylloplane of field-grown soybean every 20 days in southern buenos aires, argentina. the crop was disease-free and no pesticides were applied. out of 523 bacterial isolates (mainly gram positive bacteria) from upper and lower leaves, 134 of them were bacillus spp. bacillus pumilus was the dominant species followed by bacillus subtilis. the proportion of bacillus spp. decreased from 80% of all bacterial isolates in early stages to 0% at harvest, whereas the ...	1999	10629969
[expression of genes for guanyl-specific ribonucleases in bacillus intermedius and bacillus pumilus is regulated by the phop-phor two-component signal transduction system of pho regulon of bacillus subtilis].	promoters of the genes of guanyl-specific ribonucleases of bacillus intermedius (binase) and bacillus pumilus (rnase bp) were found to contain sequences homologous to those recognizable by the regulatory protein phop in the promoters of the pho regulon of b. subtilis, as well as regions partially homologous to the binding sites of another regulatory protein, phob, in the promoters of the pho regulon of escherichia coli. the role of the two-component regulatory systems phop-phor and phob-phor in ...	1999	10495972
the production of 'kpaye'--a fermented condiment from prosopis africana (guill and perr) taub. seeds.	'kpaye', a fermented condiment from prosopis africana seeds was produced using the traditional method. bacillus subtilis, bacillus licheniformis and bacillus pumilus were consistently isolated in the fermentations lasting 120 h. 'kpaye'production involved a rise in ph, moisture content and total free amino acids while titratable acidity and reducing sugar decreased gradually after 24 h and 72 h of fermentation respectively.	1999	10574093
the membranotropic activity of cyclic acyldepsipeptides from bacterium bacillus pumilus, associated with the marine sponge ircinia sp.	the isolate of bacillus pumilus associated with the marine sponge ircinia sp. produced the surfactin-like lipopeptides, cyclic acyldepsipeptides. the hemolytic activity of individual cyclic acyldepsipeptides, bacircines (bi) 2, 3, 4, 5 and 5a having different acyl side chain structures (anteiso-c13, iso-c14, normal-c14, anteiso-c15, and iso-c15, respectively) was studied. the hemolytic power of bacircines depended on both the structure of the side chain (n->iso->anteiso-) and ph values (5.6 and ...	1999	10219990
expression of the genes for guanyl-specific ribonucleases from bacillus intermedius and bacillus pumilus is regulated by the two component signal transduction system phop-phor in b. subtilis.	promoters of the genes for guanyl-specific ribonucleases, secreted by b. intermedius (binase) and b. pumilus (rnase bp) in phosphate deficient conditions, contain regions similar to appropriate consensus sequences in promoters of the pho regulated genes of b. subtilis. a number of genes expressed in response to phosphate starvation in b. subtilis are regulated by the two component signal transduction system phop-phor. expression of recombinant genes for binase and rnase bp in b. subtilis strains ...	1999	10220898
the influence of bacterial inoculants on the microbial ecology of aerobic spoilage of barley silage.	the aerobic decomposition of barley silage treated with two inoculants (laca and lacb) containing mixtures of lactobacillus plantarum and enterococcus faecium was investigated over a 28-day period. initially, yeast and bacterial populations were larger in silage inoculated with laca than in silage treated with lacb or water alone (control). differences in the succession of yeasts in silage treated with laca were observed relative to the other two treatments. from silage treatment with laca, issa ...	1999	10349723
electrospray ionization multiple-stage tandem mass spectrometric analysis of diglycosyldiacylglycerol glycolipids from the bacteria bacillus pumilus.	electrospray ionization (esi) combined with multiple-stage tandem mass spectrometry (ms(n)) was used to directly analyze the glycolipid mixture from bacteria bacillus pumilus without preliminary separation. full scan esi-ms revealed the composition of picomole quantities of glycerolglycolipid species containing c(14)-c(19) fatty acids, some of which were monounsaturated. two main components were identified from their molecular masses and fragmentation pathways. the fragmentation pathway of the k ...	1999	10407296
gamma irradiation for terminal sterilization of 17beta-estradiol loaded poly-(d,l-lactide-co-glycolide) microparticles.	17beta-estradiol-loaded microparticles using poly-(d, l-lactide-co-glycolide) polymer (plg) were prepared by a modified spray-drying method and the effects of gamma-irradiation on drug substance, polymer and microparticles were investigated. irradiation doses ranging from 5.1 to 26.6 kgy were applied using a 60co-radiation source. 17beta-estradiol drug substance showed excellent stability against gamma-irradiation in the investigated dose range, whereas microencapsulated estradiol seems to be co ...	1999	10469916
expression of listeriolysin o and acta by intracellular and extracellular listeria monocytogenes.	listeria monocytogenes requires listeriolysin o (llo) and acta, the products of hly and acta, respectively, to establish a productive intracellular infection. llo is essential for vacuolar lysis and entry into the cytosol, while acta is required for bacterial spread to adjacent cells. we have used a transcriptional reporter gene system to compare the expression of acta and hly during intracellular growth to that during growth in broth cultures. the hly and acta genes were transcriptionally fused ...	1999	9864207
purification and mode of action of an alkali-resistant endo-1, 4-beta-glucanase from bacillus pumilus.	alkaline endo-1,4-beta-d-glucanase was secreted by bacillus pumilus grown in submerged culture on a combination of oat spelt xylan and corn starch as carbon sources. the enzyme was purified to homogeneity by sephacryl s-200 and q-sepharose column chromatography. the protein corresponded to molecular mass and pi values of 67 kda and 3.7, respectively. the enzyme was optimally active at ph 7.0-8.0 and 60 degrees c and retained 50% of its optimum activity at ph 12. the most notable characteristic o ...	1999	10087165
[expression of secreted guanyl-specific ribonuclease genes from bacillus intermedius and bacillus pumilus in bacillus subtilis cells].	plasmids with whole genes for ribonucleases from b. intermedius (binase) and b. pumilis (rnase bp) assembled with the whole gene of barstar, a specific intracellular inhibitor, are constructed. the resultant plasmids pmz55 and pmz56 effectively express binase and rnase bp genes in b. subtilis cells. a medium for maximum expression of rnase genes by recombinant strains is developed. the expression of binase and rnase bp genes in b. subtilis cells is negatively regulated by exogenic inorganic phos ...	1999	10190104
does probiotics administration decrease serum endotoxin levels in infants?	the aim of this study was to examine whether administration of probiotics to infants can change the ratio of intestinal flora and thereby decrease serum endotoxin produced by potentially pathogenic microorganisms.	1999	10052803
purification and characterization of a soybean-milk-coagulating enzyme from bacillus pumilus tyo-67.	bacillus pumilus tyo-67 was isolated from tofu (soybean curd) as the best producer of a soybean-milk-coagulating enzyme, induced by the addition of soybean protein to the growth medium. the enzyme was purified approximately 30-fold with an 11% yield. the homogeneous preparation of the enzyme showed that it is a monomer with a molecular mass of about 30 kda and has an isoelectric point at ph 9.75. the results of amino acid composition analyses showed that the enzyme is rich in alanine, aspartic a ...	1999	10341429
[estimation of probiotics by infection model of infant rabbit with enterohemorrhagic escherichia coli o157:h7].	we examined the preventative effect against enterohemorrhagic escherichia coli o157:h7 (ehec) infection in rabbits on administration of probiotics contained in streptococcus faecalis, clostridium butyricum and bacillus mesentericus. the probiotics were administered to 5 days old japanese white rabbits throughout the experiment. inoculation of 10(6) colony forming units (cfus) of ehec per rabbit was tried 8 days after birth. three days after ehec inoculation, in the control group (not administere ...	1998	9916417
cloning and analysis of the four genes coding for bpu10i restriction-modification enzymes.	the bpu 10i r-m system from bacillus pumilus 10, which recognizes the asymmetric 5'-cctnagc sequence, has been cloned, sequenced and expressed in escherichia coli . the system comprises four adjacent, similarly oriented genes encoding two m5c mtases and two subunits of bpu 10i enase (34.5 and 34 kda). both bpu10ir genes either in cis or trans are needed for the manifestation of r. bpu 10i activity. subunits of r. bpu 10i, purified to apparent homogeneity, are both required for cleavage activity. ...	1998	9461472
gene cloning, transcriptional analysis, purification, and characterization of phenolic acid decarboxylase from bacillus subtilis.	bacillus subtilis displays a substrate-inducible decarboxylating activity with the following three phenolic acids: ferulic, p-coumaric, and caffeic acids. based on dna sequence homologies between the bacillus pumilus ferulate decarboxylase gene (fdc) (a. zago, g. degrassi, and c. v. bruschi, appl. environ. microbiol. 61:4484-4486, 1995) and the lactobacillus plantarum p-coumarate decarboxylase gene (pdc) (j.-f. cavin, l. barthelmebs, and c. diviès, appl. environ. microbiol. 63:1939-1944, 1997), ...	1998	9546183
expression of a bacillus thuringiensis delta-endotoxin gene by bacillus pumilus.	the delta-endotoxin genes from bacillus thuringiensis were introduced into a rhizosphere-inhabiting bacillus pumilus isolate to create a delta-endotoxin expression and delivery system for subterranean feeding insects such as the larvae of pale western cutworm (agrotis orthogonia morrison (lepidoptera: noctuidae)). preliminary experiments indicated that bacillus thuringiensis subsp. kurstaki cultures were toxic to pale western cutworm larvae. three different cry genes from bacillus thuringiensis ...	1998	9606908
isolation and partial characterization of antifungal metabolites of bacillus pumilus.	antifungal metabolites produced by bacillus pumilus in potato dextrose broth (pdb) were isolated from culture supernatant fluid by precipitation with ammonium sulphate. the antifungal metabolites inhibited mycelial growth of many species of aspergillus, penicillium and fusarium. they also inhibited production of aflatoxins, cyclopiazonic acid, ochratoxin a and patulin. the metabolites were heat-stable and remained active after sterilization at 121 degrees c for 15 min. their activity was stable ...	1998	9717280
purification and characterization of an acetyl xylan esterase from bacillus pumilus.	bacillus pumilus ps213 was found to be able to release acetate from acetylated xylan. the enzyme catalyzing this reaction has been purified to homogeneity and characterized. the enzyme was secreted, and its production was induced by corncob powder and xylan. its molecular mass, as determined by gel filtration, is 190 kda, while sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed a single band of 40 kda. the isoelectric point was found to be 4.8, and the enzyme activity was optimal a ...	1998	10215579
phytochemical and antimicrobial studies of salvia splendens sello.	the phytochemical investigation of antimicrobial agents from locally occurring salvia splendens sello, was carried out. three common solvents i.e. petroleum ether (40-60%), chloroform and methanol were successively used for the extraction of antimicrobial principles from its various parts. three major compounds (namely c1, c2, and c3) were isolated and purified from the active methanol extract of roots by column and thin layer chromatography. gram-positive bacteria (bacillus pumilus, b. subtilis ...	1998	16414814
mixtures of plant growth-promoting rhizobacteria enhance biological control of multiple cucumber pathogens.	abstract plant growth-promoting rhizobacteria (pgpr) strains inr7 (bacillus pumilus), gb03 (bacillus subtilis), and me1 (curtobacterium flaccumfaciens) were tested singly and in combinations for biological control against multiple cucumber pathogens. investigations under greenhouse conditions were conducted with three cucumber pathogens-colletotrichum orbiculare (causing anthracnose), pseudomonas syringae pv. lachrymans (causing angular leaf spot), and erwinia tracheiphila(causing cucurbit wilt ...	1998	18944848
inhibition of aflatoxin production of aspergillus parasiticus nrrl 2999 by bacillus pumilus.	six isolates of bacillus pumilus were tested for their ability to inhibit aflatoxin production of aspergillus parasiticus nrrl 2999 in yeast extract sucrose (yes) broth. aflatoxin production was inhibited in both simultaneous and deferred antagonism assays, suggesting that the inhibitory activity was due to extracellular metabolite(s) produced in cell-free supernatant fluids of cultured broth. the inhibition was not due to organic acids or hydrogen peroxide produced by b. pumilus since the inhib ...	1997	16284816
[fermentation conditions of engineering strain utilizing starch for production of alkaline proteinase].	the highest activity of alkaline proteinase produced by bacillus pumilus c172(pbx 96) transformant was 9,000 u/ml in shaking flask where corn meal and bean cake meal were carbon and nitrogen sources, respectively. the enzyme activity was raised under the conditions of initial ph 7.0, mgcl2 instead of mgso4, and glucose (0.1%) in the substrate. the fermentation of c172(pbx 96) transformant was carried out with parameters of ph, reducing sugar, total sugar and enzyme activity.	1997	11189381
inhibition of aflatoxin production of aspergillus parasiticus nrrl 2999 by bacillus pumilus.	six isolates of bacillus pumilus were tested for their ability to inhibit aflatoxin production of aspergillus parasiticus nrrl 2999 in yeast extract sucrose (yes) broth. aflatoxin production was inhibited in both simultaneous and deferred antagonism assays, suggesting that the inhibitory activity was due to extracellular metabolites(s) produced in cell-free supernatant fluids of cultured broth. the inhibition was not due to organic acids or hydrogen peroxide produced by b. pumilus since the inhi ...	1997	9735015
cloning and expression of the glutamate racemase gene of bacillus pumilus.	a glutamate racemase gene (muri) was found in bacillus pumilus cells and cloned into escherichia coli wm335, a d-glutamate auxotroph, by means of a genetic complement method. muri of b. pumilus encodes a 272-amino acid protein with an unusual initiation codon, ttg. the deduced amino acid sequence shows significant similarity with those of glutamate racemases from e. coli (ratio of identical residues, 28%), pediococcus pentosaceus (44%), and staphylococcus haemolyticus (49%). b. pumilus muri was ...	1997	9354391
bpli, a new bcgi-like restriction endonuclease, which recognizes a symmetric sequence.	bcg i and bcg i-like restriction endonucleases cleave double stranded dna specifically on both sides of their asymmetric recognition sequences which are interrupted by several ambiguous base pairs. their heterosubunit structure, bifunctionality and stimulation by adomet make them different from other classified restriction enzymes. here we report on a new bcg i-like restriction endonuclease, bpl i from bacillus pumilus , which in contrast to all other bcg i-like enzymes, recognizes a symmetric i ...	1997	9358150
[immune reaction to the heterologic test-antigen in mice with anti-cancer vaccine].	the stimulated influence of the antitumoral vaccine, prepared with the help of filtrate of the bacillus mesentericus ab-56 or the antibiotic, extracted from it, on the immune answer to heterologic test-antigen (ram erythrocytes) was established. while the vaccine injection to laboratory animals with developing tumor their capacity to resist to the tumors was rising up.	1997	9615014
leader peptides of inducible chloramphenicol resistance genes from gram-positive and gram-negative bacteria bind to yeast and archaea large subunit rrna.	cata86 is the second gene in a constitutively transcribed, two-gene operon cloned from bacillus pumilus . the region that intervenes between the upstream gene, termed the leader, and the cata86 coding sequence contains a pair of inverted repeat sequences which cause sequestration of the cata86 ribosome binding site in mrna secondary structure. as a consequence, the cata86 coding sequence is untranslatable in the absence of inducer. translation of the cata86 coding sequence is induced by chloramp ...	1997	9108153
cloning of the bacillus pumilus beta-xylosidase gene (xynb) and its expression in saccharomyces cerevisiae.	a genomic dna library of the bacterium bacillus pumilus pls was constructed and the beta-xylosidase gene (xynb) was amplified from a 3-kb genomic dna fragment with the aid of the polymerase chain reaction technique. the amplified xynb gene was inserted between the yeast alcohol dehydrogenase ii gene promoter (adh2p) and terminator (adh2t) sequences on a multicopy episomal plasmid (pdlg11). the xynb gene was also fused in-frame to the secretion signal sequence of the yeast mating pheromone alpha- ...	1997	9114518
purification and characterization of a dipeptidyl carboxypeptidase from pseudomonas sp. wo24.	a dipeptidyl carboxypeptidase (dcp) activity was detected in cell-free extracts of pseudomonas sp. wo24. after purification and characterization the enzyme was found to be homogeneous by sds-page, and had a molecular mass of 74,000 da by sds-page and 72,000 da by gel filtration, indicating that it is monomeric. the isoelectric point was 5.2 and optimum ph was 6.5-7.0. it showed a specific activity of 780 mumol/min/mg, which is the highest of the values shown by known enzymes. the enzyme hydrolyz ...	1997	9178563
investigation of pulsed light for terminal sterilization of wfi filled blow/fill/seal polyethylene containers.	a study was performed to assess the ability of pulsed light to sterilize water for injection in blow/fill/seal polyethylene containers. pulsed light uses intense, short duration flashes of broad spectrum white light to produce high levels of microbial kill. in a first phase of testing, containers of 0.5, 5, 15, and 120 ml nominal volume were inoculated with bacillus pumilus endospores, bacillus subtilus variety niger strain globigii endospores, bacillus stearothermophilus endospores, and aspergi ...	1997	9203823
effect of high-power microwave on indicator bacteria for sterilization.	according to the superiority sterilization, a specially-designed microwave disinfector using high-power microwave energy was made. a series of sterilizing experiments have been made to determine the effect of microwave energy on several typical indicator bacteria such as bacillus subtilis var. niger, bacillus stearothermophilus, bacillus pumilus e601, staphylococcus aureus, bacillus cereus. under the conditions of different sterilization duration and unequal intensity of microwave power irradiat ...	1996	9216147
simple detection of xylosidase activity in single colonies, using 1-naphthyl-beta-d-xylopyranoside.	since the xylosidase of bacillus pumilus hydrolyzed 1-naphthyl-beta-d-xylopyranoside (naphthyl-x) to produce xylose and 1-naphthol and a chromogenic azo compound is produced by coupling 1-naphthol and fast blue salt b, a simple method for detection of xylosidase activity in single colonies was studied. escherichia coli jm109 carrying the xylosidase gene of b. pumilus was cultivated at 37 degrees c for 18 h on a lb plate containing 0.5 mg/ml naphthyl-x, and then the plate was overlaid with 3 ml o ...	1996	8987608
[effect of culture medium components on the accumulation of extracellular bacillary ribonucleases in culture fluid of recombinant strains of escherichia coli].	the effect of the concentrations of peptone, yeast extract, and inorganic phosphate on the expression of genes of extracellular ribonucleases from bacillus intermedius 7p (binase) and bacillus amyloliquefaciens h2 (barnase) was studied in escherichia coli cells transformed with plasmids containing the structural genes of binase or barnase under the control of their own or synthetic regulatory region or the structural binase gene under the control of the regulatory regions of the genes of barnase ...	1996	9102546
isolation of a gene essential for biosynthesis of the lipopeptide antibiotics plipastatin b1 and surfactin in bacillus subtilis yb8.	bacillus subtilis yb8 was found to produce the lipopeptide antibiotics surfactin and plipastatin b1. a gene, lpa-8, required for the production of both lipopeptides was cloned from strain yb8. when this gene was inactivated in strain yb8, neither surfactin nor plipastatin b1 was produced. however, the defective strain transformed with an intact lpa-8 gene had restored ability to produce both peptides. nucleotide sequence analysis of the region essential for the production of the peptides reveale ...	1996	8639027
note: microbial resistance of wool fabric treated with bis-quats compounds.	in this paper, the antibacterial activity against bacillus pumilus, staphylococcus aureus, escherichia coli and pseudomonas aeruginosa of the wool fabrics treated with new antimicrobial bis-quaternary surfactants, dabk and dabb, is studied. the activity was established on the basis of the agar diffusion and protective antibacterial test results and on the basis of scanning electron microscopy (sem) observation. the results were compared with the htab, a monoquaternary surfactant of conventional ...	1996	8760331
in vitro studies of the loss of antibacterial activity of oxytetracycline in presence of ca(ii) or mg(ii) ions.	the results of a comparative study, which evaluated the in vitro effect on the antibacterial activity of oxytetracycline (otc, cas 79-57-2) in presence of ca(ii)/mg(ii) ions suggest that susceptibility of staphylococcus aureus, bacillus pumilis and bacillus subtilis to otc is reduced in presence of ca(ii)/mg(ii) ions. as the ratio of concentration of ca(ii)/mg(ii) to otc was increased, antibacterial activity of otc declined. in addition to the difference observed between the antibacterial effect ...	1996	8842342
comparison of the properties of native and pentaammineruthenium(iii)-modified xylanase.	two xylanases, xyna of bacillus pumilus and xyn ii of trichoderma reesei, were purified and then modified by the attachment of pentaammineruthenium, thereby resulting in the generation of a xylanase with veratryl alcohol oxidase activity. hydrolytic activity of t. reesei xyn ii on soluble xylans was unchanged by modification with pentaammineruthenium; however, modification of b. pumilus xyna greatly reduced xylan hydrolysis unless the active site of the xylanase was protected with xylose during ...	1996	8987538
a simple assay for xylanase using o-nitrophenyl-beta-d-xylobioside.	we measured xylanase activities upon eight chromogenic substrates, o- or p-nitrophenyl-beta-d-xylopyranoside (onp-x or pnp-x) and o- or p-nitrophenyl-beta-d-xylooligosaccharides (onp-xn or pnp-xn, n = 2-4), and studied for their uses as substrates in a kinetic study. the kcat and k(m) of bacillus pumilus xylanase (ec 3.2.1.8) activities for pnp-x2 were 0.24s-1 and 0.5 mm, respectively. the relative xylanase activities with the other substrates to that with pnp-x2, pnp-x3, pnp-x4, onp-x, onp-x2, ...	1996	8695915
two related rolling circle replication plasmids from salt-tolerant bacteria.	in a range of salt-tolerant bacteria isolated from soil, rhizosphere, and phyloplane, cross-hybridization tests revealed a group of seven plasmids which were chosen for further study. two plasmids (psh1418 and psh1451) were picked as representative examples of the two related subgroups. restriction mapping and southern blotting identified a region common to the two plasmids which later was identified as encoding the replication functions. we were unable to join these plasmids to high-copy-number ...	1996	9007014
induction of defense-related ultrastructural modifications in pea root tissues inoculated with endophytic bacteria.	the stimulation exerted by the endophytic bacterium bacillus pumilus strain se34 in plant defense reactions was investigated at the ultrastructural level using an in vitro system in which root-inducing t-dna pea (pisum sativum l.) roots were infected with the pea root-rotting fungus fusarium oxysporum f. sp. pisi. in nonbacterized roots, the pathogen multiplied abundantly through much of the tissue including the vascular stele, whereas in prebacterized roots, pathogen growth was restricted to th ...	1996	12226427
phenol and cresol metabolism in bacillus pumilus isolated from contaminated groundwater.	from an aquifier contaminated with phenolic compounds seven bacterial strains able to grow on phenol and several mono- and disubstituted alkylphenols as sole source of carbon and energy were isolated. five isolates belong to the genus pseudomonas, two to the genus bacillus. the isolate most active in utilization of the applied xenobiotics was identified as bacillus pumilus and used for the investigation of the degradation pathways in liquid cultures. cells of this strain precultured on phenol we ...	1995	7783002
machine operator's lung. a hypersensitivity pneumonitis disorder associated with exposure to metalworking fluid aerosols.	six auto parts manufacturing workers were referred for evaluation of a 6-week history of work-related dyspnea, cough, and fatigue. two workers also reported fever and weight loss. all six worked in a machining area where a waterbased metalworking fluid was used and recirculated under high pressure, thereby creating an aerosol. chest radiographs revealed pulmonary interstitial infiltrates in four workers. lung function tests showed that four workers had decreased diffusing capacity. after removal ...	1995	7656609
antibiotic production and biocontrol activity by bacillus subtilis cl27 and bacillus pumilus cl45.	bacillus subtilis cl27 and b. pumilus cl45 showed similar activity against botrytis cinerea in in vitro plate assays. in a seedling bioassay, however, b. subtilis cl27 had activity similar to a commercial fungicide while b. pumilus cl45 failed completely to prevent seedling damping-off caused by bot. cinerea. antibiotic production by the two bacillus strains was found to depend on the growth substrate and highest antibiotic production was found on media based on homogenized cabbage tissue. antib ...	1995	7698955
cloning, sequencing, and expression in escherichia coli of the bacillus pumilus gene for ferulic acid decarboxylase.	the bacillus pumilus gene encoding a ferulic acid decarboxylase (fdc) was identified and isolated by its ability to promote ferulic acid decarboxylation in escherichia coli dh5 alpha. the dna sequence of the fdc gene was determined, and the recombinant enzyme produced in e. coli was purified and characterized.	1995	8534115
the mtrb gene of bacillus pumilus encodes a protein with sequence and functional homology to the trp rna-binding attenuation protein (trap) of bacillus subtilis.	the mtrb gene from bacillus pumilus encodes a 76-amino-acid polypeptide with 77% identity to the trp rna-binding attenuation protein (trap) from bacillus subtilis. b. pumilus trap binds trp leader rna from either b. subtilis or b. pumilus in a tryptophan-dependent manner. altering threonine 52 to alanine eliminated rna-binding activity of b. pumilus trap.	1995	7836324
purification and characterization of ferulate and p-coumarate decarboxylase from bacillus pumilus.	bacillus pumilus ps213 isolated from bovine ruminal fluid was able to transform ferulic acid and p-coumaric acid to 4-vinylguaiacol and 4-vinylphenol, respectively, by nonoxidative decarboxylation. the enzyme responsible for this activity has been purified and characterized. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of crude extract from a culture induced by ferulic acid or p-coumaric acid shows three bands that are not present in the crude extract of an uninduced culture, while ...	1995	7887611
phosphate regulation of biosynthesis of extracellular rnases of endospore-forming bacteria.	the gene for the extracellular ribonuclease of b. pumilus kmm62 (rnase bp) has been cloned and sequenced. the structural gene for this enzyme is similar to those of the extracellular ribonucleases of b. intermedius 7p (binase) and b. amyloliquefaciens h2 (barnase), as are the regulatory regions of binase and rnase bp. the regulatory region of the barnase gene, however, is quite different from the other two. in the promoter of the genes for binase and rnase bp, but not in that for barnase, is a r ...	1995	8001670
[biosynthetic regulation of extracellular ribonucleases in native strains of bacilli and in recombinant strains of escherichia coli].	regulation of the biosynthesis of extracellular ribonucleases of bacillus amyloliquefaciens h2 (barnase), bacillus intermedius 7p (binase), and bacillus pumilus kmm 62 (rnase bp) was studied in their native strains and recombinant escherichia coli strains. recombinant plasmids were obtained that contained genes encoding barnase, binase, and rnase bp under the control of their own regulatory sequences (plasmids pmt415, pml5, and pml61), genes encoding barnase and binase under the control of the t ...	1995	8538511
mechanism of inhibited growth of bacillus pumilus by propionibacterium freudenreichii subsp. shermanii.	physiological studies were conducted in an attempt to elucidate the mechanism of inhibition of bacillus pumilus by propionibacterium freudenreichii subsp. shermanii. inhibition of b. pumilus by p. shermanii occurred in media supplemented with 1% glucose, indicating that glucose utilization by the latter bacterium was not responsible for growth inhibition of the former bacterium. the medium ph in which p. shermanii inhibited the growth of b. pumilus was 4.3. propionic acid was positively identifi ...	1994	8060789
specificity of chitosanase from bacillus pumilus.	partially (25-35%) n-acetylated chitosan was digested by chitosanase from bacillus pumilus bn-262, and structures of the products, partially n-acetylated chitooligosaccharides, were analyzed in order to investigate the specificity of the chitosanase. the chitosanase produced glucosamine (glcn) oligosaccharides abundantly, indicating that the chitosanase splits the beta-1,4-glycosidic linkage of glcn-glcn. the chitosanase also produced hetero-oligosaccharides consisting of glucosamine and n-acety ...	1994	8155696
gamma sterilization of a semi-solid poly(ortho ester) designed for controlled drug delivery--validation and radiation effects.	radiation sterilization is becoming increasingly popular for the sterilization of many pharmaceutical products. although this technique is not limited to the sterilization of polymers, it is probably the most suitable method for such materials. this method however suffers several drawbacks. the sterilization of a product must lead to a safety level of 10(-6), i.e. one chance in a million to find a contaminated sample. in many cases, this assurance of sterility can be achieved by using a uniform ...	1994	7855057
[biosynthesis of extracellular ribonuclease by bacillus pumilus].	conditions for the biosynthesis of alkaline extracellular ribonuclease by bacillus pumilus were studied and their comparison with that of the biosynthesis of ribonuclease by bacillus intermedius was made. it was estimated that ribonuclease of bacillus pumilus is synthesized post-exponentially, its synthesis is inhibited by inorganic phosphate and is stimulated by actinomycin d, as the synthesis of ribonuclease by bacillus intermedius. at the same time essential differences in dynamic of enzyme f ...	1994	7760766
comparative effects of gamma rays and electron beams on spores of bacillus pumilus.	the effects of gamma rays and electron beams on the germination, outgrowth and the synthesis of protein and rna of bacillus pumilus spores were investigated to clarify the difference in the effects of the two types of radiations on bacterial spores. gamma irradiation facilitated the germination to a slightly larger degree than electron irradiation. the outgrowth, growth and the synthesis of protein and rna were inhibited by gamma irradiation to a greater extent than electron irradiation, when th ...	1994	7510894
disinfection efficacy in an integrated ultraviolet light contact lens care system.	we evaluated a new integrated contact lens care system that combines fluid turbulence for lens cleaning with ultraviolet (uv) light for solution sterilization. the ultraviolet light system was used to clean and disinfect 42 soft contact lenses (water contents: 38.6%, 43%, 55%, and 70%) and two rigid gas permeable lenses. test lenses were contaminated with 10(6) cells/ml of bacillus pumilus, aspergillus niger, pseudomonas aeruginosa, and acanthamoeba castellanii and subjected to a 15-minute clean ...	1994	7820920
bacillus dna in fossil bees: an ancient symbiosis?	we report here the isolation of dna from abdominal tissue of four extinct stingless bees (proplebeia dominicana) in dominican amber, pcr amplification of a 546-bp fragment of the 16s rrna gene from bacillus spp., and their corresponding nucleotide sequences. these sequences were used in basic local alignment search tool searches of nonredundant nucleic acid data bases, and the highest scores were obtained with 16s rrna sequences from bacillus spp. phylogenetic inference analysis by the maximum-l ...	1994	8031102
distribution of alginate genes in bacterial isolates from corroded metal surfaces.	the distribution of alginate genes encoding biosynthesis of alginate was examined for bacterial isolates associated with corrosive biofilms recovered from source water, cooling lines, and reactor surfaces of a nuclear power plant. a total of 120 diverse gram-positive and -negative isolates were obtained. using dna:dna hybridization, 11 isolates were shown to contain sequences homologous to structural (algd, algg, alg-76) and/or regulatory (albb) alginate biosynthetic genes derived from an algina ...	1994	24190336
nucleotide sequence of the clostridium stercorarium xyna gene encoding xylanase a: identification of catalytic and cellulose binding domains.	the nucleotides of the xyna gene of clostridium stercorarium were sequenced. the structural gene consists of an open reading frame of 1533 bp encoding 511 amino acids with an m(r) of 56,519. the signal peptide cleavage site was identified by comparison with the n-terminal amino acid sequence of the enzyme produced by a recombinant escherichia coli. xylanase a consists of a catalytic domain belonging to family g at the n-terminus and two direct repeats of about 90 amino acids with a short spacing ...	1993	7763496
isolation and characterization of a cyanide dihydratase from bacillus pumilus c1.	a cyanide-degrading enzyme from bacillus pumilus c1 has been purified and characterized. this enzyme consisted of three polypeptides of 45.6, 44.6, and 41.2 kda; the molecular mass by gel filtration was 417 kda. electron microscopy revealed a multimeric, rod-shaped protein approximately 9 by 50 nm. cyanide was rapidly degraded to formate and ammonia. enzyme activity was optimal at 37 degrees c and ph 7.8 to 8.0. activity was enhanced by sc3+, cr3+, fe3+, and tb3+; enhancement was independent of ...	1993	8407782
a new antibacterial sesquiterpene from premna oligotricha.	a novel sesquiterpene, 7 alpha-hydroxy-6, 11-cyclofarnes-3(15)-en-2-one [1], has been isolated from the aerial parts of premna oligotricha (verbenaceae) using an antimicrobial bioassay-guided isolation procedure. the sesquiterpene was identified on the basis of spectroscopic data and showed weak activity against gram-positive bacteria bacillus pumilus, bacillus subtilis, staphylococcus aureus, and streptococcus faecalis.	1993	8450316
the xync gene from fibrobacter succinogenes s85 codes for a xylanase with two similar catalytic domains.	the xync gene of fibrobacter succinogenes s85 codes for a 66.4-kda xylanase which consists of three distinct domains separated by two flexible regions rich in serine residues. domains a and b of xync code for catalytic domains with 56.5% identity and 9.6% similarity with each other, and both domains share homology with xylanases of ruminococcus flavefaciens, neocallimastix patriciarum, clostridium acetobutylicum, bacillus pumilus, bacillus subtilis, and bacillus circulans. more than 88% of the x ...	1993	8244936
[metalloproteinase of bacillus mesentericus, strain v-313].	a homogeneous metalloproteinase has been isolated with a 28% yield from the culture fluid of bacillus mesentericus, strain b-313. the isolation procedure included chromatography on bacitracin-silochrome and gel filtration on acrylex p-10 and sephadex g-75. the enzyme has a molecular mass of 41,000 da; its n-terminal sequence, which appears as a-a-t-t-g-t-g-t-t-l-k-g-k-t-v-s-l-n-i, is identical with that of the b. amyloliquefaciens enzyme. like other metalloproteinases, the enzyme is inhibited by ...	1993	8218566
interactive behavior of saccharomyces cerevisiae, bacillus pumilus and propionibacterium freudenreichii subsp. shermanii.	prevention of ropy bread caused by mucoid variants of certain bacilli presents a major problem for developing countries where cost of preservatives is prohibitive. control of ropiness may be achieved by using propionic acid-producing bacteria in mixed culture with leavening yeasts. therefore, interaction studies between propionibacterium freudenreichii subsp. shermanii, bacillus pumilus and saccharomyces cerevisiae were conducted in a chemically defined medium to test the relevance of such an ap ...	1993	8257655
purification and some properties of an alkaline xylanase from alkaliphilic bacillus sp. strain 41m-1.	an alkaliphilic bacillus sp. strain, 41m-1, isolated from soil produced multiple xylanases extracellularly. one of these xylanases was purified to homogeneity by ammonium sulfate fractionation and anion-exchange chromatography. the moleculr mass of this enzyme (xylanase j) was 36 kda, and the isoelectric point was ph 5.3. xylanase j was most active at ph 9.0. the optimum temperature for the activity at ph 9.0 was around 50 degrees c. the enzyme was stable up to 55 degrees c at ph 9.0 for 30 min. ...	1993	8292206
kinetic characterization of alkaline mesentericopeptidase. comparison with serine proteinases from different origins.	comparative studies of the hydrolysis of succinyl-ala2-phe-methylcoumarylamide with mesentericopeptidase, a mesophilic extracellular serine proteinase from bacillus mesentericus, and proteinases produced by organisms representing different levels of evolutionary development, were performed. drastic differences in the proteolytic coefficient kcat/km were found. as regards their catalytic efficiency, the proteinases studied can be placed in the following order: mesentericopeptidase < subtilisin no ...	1993	8307687
[h2o2 low temperature plasma sterilization. new possibilities for use with eye surgery instruments].	the h2o2-low-temperature-plasma-sterilization (sterrad 100) works with a temperature below 50 degrees c (140 degrees f). this system is appliable for thermostabile materials as well as for thermolabile materials. the efficancy of this new system is shown by a biological test with bacillus pumilus spores. 5 typical ophthalmic surgical instruments were contaminated. after sterilization the numerical reduction of the microorganisms had to be at least 6 log levels. corrosion caused by hydrogene pero ...	1993	8124049
hydrolysis of glycosylpyridinium ions by anomeric-configuration-inverting glycosidases.	the hydrolyses of five beta-d-xylopyranosylpyridinium ions by the beta-d-xylosidase of bacillus pumilus proceed with kcat values 10(8)-10(9)-fold larger than the rates of spontaneous hydrolysis of the same compounds. log(kcat) values correlate well with aglycon pk(a) [b1g(v) = -0.52, r = 0.99], whereas the correlation of log(kcat/km) is poor [r = 0.77; beta 1g(v/k) = approximately -0.6]. the (1-->3)-beta-d-glucanase of sporotrichum dimorphosporum hydrolyses 4-bromo-2-(beta-d-glucopyranosyl)isoqu ...	1993	8143292
the effect of ribosomal protein s1 from escherichia coli and micrococcus luteus on protein synthesis in vitro by e. coli and bacillus subtilis.	we have designed a set of nine plasmids containing the bacillus pumilis cat gene with one of three shine-dalgarno (sd) sequences (weak, strong or stronger) and one of three initiation codons (aug, gug or uug). these constructions have been used to determine the effect of ribosomal protein s1, sd and initiation codon sequences and escherichia coli ribosomal protein s1 on translation in vitro by e. coli and b. subtilis ribosomes. translation of these nine constructions was determined with three ty ...	1992	1283001
[extracellular ribonuclease from bacillus pumilus].	the extracellular ribonuclease (rnase bp) was isolated from the cultural medium filtrate of bacillus pumilus by ammonium sulfate precipitation and two stages of ion-exchange chromatography on carboxymethyl- and phospho-cellulose columns. the amino acid composition and n-terminal amino acid residue have been determined. the kinetic parameters of cleavage reaction of synthetic polynucleotides have been measured. according to their structural homology rnase bp has been shown to be similar to rnases ...	1992	1302501
[transposition of tn917 in bacillus pumilus].	transposition tn917 was introduced into bacillus pumilus 289 by protoplast transformation with plasmid ptv32. the temperature-sensitive replication property of ptv32 was maintained in b. pumilus. tn917 was transposed efficiently in b. pumilus with 4.8 x 10(-4) transposition rate. the yield of auxotrophs was about 0.65% in all insertional mutants. it indicated a prospects for the use of tn917 as a tool for insertional mutagenesis and genetic manipulation in b. pumilus.	1992	1329360
site-directed mutagenesis at aspartate and glutamate residues of xylanase from bacillus pumilus.	to elucidate the reaction mechanism of xylanase, the identification of amino acids essential for its catalysis is of importance. studies have indicated the possibility that the reaction mechanism of xylanase is similar to that of hen's egg lysozyme, which involves acidic amino acid residues. on the basis of this assumption, together with the three-dimensional structure of bacillus pumilus xylanase and its amino acid sequence similarity to other xylanases of different origins, three acidic amino ...	1992	1359880
bpuai, a novel bbsi and bbvii isoschizomer from bacillus pumilus recognizing 5'-gaagac-3'.		1992	1408773
purification, properties, and partial amino acid sequences of thermostable xylanases from streptomyces thermoviolaceus opc-520.	two types of xylanases (1,4-beta-d-xylan xylanohydrolase, ec 3.2.1.8) were isolated from the culture filtrate of a thermophilic actinomycete, streptomyces thermoviolaceus opc-520. the enzymes (stx-i and stx-ii) were purified by chromatography with deae-toyopearl 650 m, cm-toyopearl 650 m, sephadex g-75, phenyl-toyopearl 650 m, and mono q hr. the purified enzymes showed single bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the molecular weights of stx-i and stx-ii were 54,000 ...	1992	1539982
systemic bacillus species infection mimicking listeriosis of pregnancy.	bacillus species are increasingly recognized as agents of infection in humans. these organisms are ubiquitous in nature and can cause clinical illness ranging from transient bacteremia to serious systemic infection. we describe a pregnant intravenous drug abuser with fever, constitutional symptoms, and premature labor. her blood cultures yielded gram-positive bacilli, and her clinical course was consistent with systemic listeriosis of pregnancy. pathological examination of the placenta revealed ...	1992	1562662
a bifunctional xylanase encoded by the xyna gene of the rumen cellulolytic bacterium ruminococcus flavefaciens 17 comprises two dissimilar domains linked by an asparagine/glutamine-rich sequence.	the nucleotide sequence of the xyna gene of ruminococcus flavefaciens 17 was determined and found to consist of a 2862bp open reading frame beginning with a ttg start codon. the predicted product, xyla, consisted of distinct amino-terminal (a) and carboxy terminal (c) domains (248 amino acids, including a putative signal sequence, and 332 amino acids, respectively) linked by a repetitive sequence (b, 374 amino acids) extraordinarily rich in asparagine (45%) and glutamine (26%) residues. domains ...	1992	1584021
complex between the subtilisin from a mesophilic bacterium and the leech inhibitor eglin-c.	the alkaline proteinase from the mesophilic bacterium bacillus mesentericus has been crystallized in a 1:1 complex with the inhibitor eglin-c from the medical leech. the crystals have cell dimensions of a = 43.0, b = 71.9, c = 48.3 a and beta = 110.0 degrees and are in the space group p2(1). three-dimensional data to 2.0 a have been recorded on film from a single crystal. the orientation and position of the complex in the unit cell have been established using the refined coordinates of subtilisi ...	1991	1793542
sequence and properties of beta-xylosidase from bacillus pumilus ipo. contradiction of the previous nucleotide sequence.	the nucleotide sequence of the beta-xylosidase (xynb) gene from bacillus pumilus has been reported previously [moriyama, h., fukusaki, e., crespo, j.c., shinmyo, a. & okada, h. (1987) eur. j. biochem. 166, 539-545]. however, the sequence identified in the present study is quite different from the previously reported one. the total length of the psti--ecori fragment of a plasmid poxn295 containing the xynb gene is 2201 bp from our sequencing, while the length of the fragment in the previous data ...	1991	1765080
isolation and characterization of chromosomal promoters of streptococcus salivarius subsp. thermophilus.	a promoter probe vector, ptg244, was constructed with the aim of isolating transcription initiation signals from streptococcus thermophilus (streptococcus salivarius subsp. thermophilus). ptg244 is based on the escherichia coli-streptococcus shuttle vector ptg222, into which the promoterless chloramphenicol acetyltransferase gene of bacillus pumilus (cat-86) was cloned. random sau3a fragments from the s. thermophilus a054 chromosomal dna were cloned upstream of the cat-86 gene by using e. coli a ...	1991	1854195
modification of a zinc proteinase from bacillus mesentericus strain 76 by diethylpyrocarbonate.	diethylpyrocarbonate (depc) inactivated the neutral zinc proteinase from bacillus mesentericus strain 76/bacillus subtilis (mcp 76) by ethoxycarbonylation completely. exposure of the enzyme to depc together with the competitive inhibitor z-l-phenylalanine prevented the loss of activity toward both peptide and protein substrates. treatment with hydroxylamine restored the catalytic properties of the modified mcp 76 to that of the native enzyme. after chymotryptic digestion of ethoxycarbonylated mc ...	1991	1894447