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identification of the potential of microbial combinations obtained from spent mushroom cultivation substrates for use in textile effluent decolorization.the study presents variation in microbial population of agaricus bisporus, pleurotus sajor-caju and volvariella volvacea spent substrates (sms) along with ligninolytic enzymes activity and textile effluent decolorization potential of microorganisms isolated from these. the effect of temperature, ph, carbon sources and immobilizing agents on effluent decolorization using different combinations of these microorganisms has also been studied. sms of p. sajor-caju harbored highest population and dive ...201223026337
[cell wall teichoic acids of bacillus licheniformis vkm b-511t, bacillus pumilus vkm b-508t, and other strains previously assigned to bacillus pumilus]. 201223156690
swapping of pro-sequences between keratinases of bacillus licheniformis and bacillus pumilus: altered substrate specificity and thermostability.pro-sequences were swapped in cis between keratinases from bacillus licheniformis (ker bl) and bacillus pumilus (ker bp) to construct ker probp-bl and ker probl-bp, respectively. expression of these keratinases was carried out constitutively by e. coli hb101-pezz18 system. they were characterized with respect to their parent enzymes, ker bl and ker bp, respectively. ker probp-bl became more thermostable with a t(1/2) of 45 min at 80°c contrary to ker bl which was not stable beyond 60°c. similarl ...201222759531
γ-glutamyl transpeptidase from bacillus pumilus ks 12: decoupling autoprocessing from catalysis and molecular characterization of n-terminal region.gamma glutamyl transpeptidase from bacillus pumilus ks12 (ggtbp) was cloned, expressed in pet-28-e. coli expression system as a heterodimeric enzyme with molecular weights of 45 and 20 kda for large and small subunit, respectively. it was purified by nickel affinity chromatography with hydrolytic and transpeptidase activity of 1.82 u/mg and 4.35 u/mg, respectively. sequence analysis revealed that ggtbp was most closely related to bacillus licheniformis ggt and had all the catalytic residues and ...201222305170
diversity of spore-forming bacteria and identification of bacillus amyloliquefaciens as a species frequently associated with the ropy spoilage of bread.this study examines the diversity of spore-forming bacteria isolated from raw materials/bread using molecular methods along with a rapid and innovative technology, the ft-nir spectroscopy. microbiological analysis showed that 23% of semolina and 42% of other raw materials (including grain, brewer yeast, improvers) contained more than 100 spores/g and more than 50% of each kind of sample was contaminated at a level ranging from 1 to 100 spores/g. a high bacterial diversity characterized raw mater ...201222551674
chitin extraction from shrimp shell waste using bacillus bacteria.the ability of six protease-producing bacillus species (bacillus pumilus a1, bacillus mojavencis a21, bacillus licheniformis rp1, bacillus cereus sv1, bacillus amyloliquefaciens an6 and bacillus subtilis a26) to ferment media containing only shrimp shell waste, for chitin extraction, was investigated. more than 80% deproteinization was attained by all the strains tested. however, demineralization rates not exceeding 67% were registered. cultures conducted in media containing shrimp shell waste s ...201222981824
capability of a selected bacterial consortium for degrading diesel/biodiesel blends (b20): enzyme and biosurfactant production.the search for alternative sources of energy, such as biodiesel, has been stimulated, since this biofuel is highly susceptible for biodegradation and has low toxicity, thus, reducing the impact in ecosystems. the objective of this study was to select a bacterial consortium with potential for degrading diesel/biodiesel blends (b20) obtained from areas contaminated with hydrocarbons/esters. in order to evaluate the biodegrability of the blend, six enzyme assays were conducted: alkane hydroxylase, ...201222755524
complete genome of bacillus pumilus siphophage riggi.bacillus pumilus is primarily used in the agricultural industry to promote plant growth and provide resistance to bacterial and fungal plant diseases. it has recently, however, been shown to cause disease in humans. here, we announce the complete genome of b. pumilus phage riggi.201324309728
complete genome of bacillus pumilus siphophage glittering.bacillus pumilus is a gram-positive bacterium widely used in agriculture both as an antifungal and as a growth-promoting symbiont. b. pumilus is rarely infectious but has recently been shown to infect humans. here, we present the complete genome of b. pumilus phage glittering, a potential biocontrol agent for b. pumilus.201324309725
complete genome of bacillus pumilus siphophage blastoid.phage blastoid is a siphophage that infects bacillus pumilus. b. pumilus is widely used in agriculture but has recently been linked to cases of food poisoning. here, we report the complete genome of blastoid and discuss unique genomic characteristics.201324309724
phylogenetic diversity of the bacillus pumilus group and the marine ecotype revealed by multilocus sequence analysis.bacteria closely related to bacillus pumilus cannot be distinguished from such other species as b. safensis, b. stratosphericus, b. altitudinis and b. aerophilus simply by 16s rrna gene sequence. in this report, 76 marine strains were subjected to phylogenetic analysis based on 7 housekeeping genes to understand the phylogeny and biogeography in comparison with other origins. a phylogenetic tree based on the 7 housekeeping genes concatenated in the order of gyrb-rpob-pyca-pyre-mutl-aroe-trpb was ...201324244618
biodegradation of low-density polyethylene by marine bacteria from pelagic waters, arabian sea, india.sixty marine bacteria isolated from pelagic waters were screened for their ability to degrade low-density polyethylene; among them, three were positive and able to grow in a medium containing polythene as the sole carbon source. the positive isolates were identified as kocuria palustris m16, bacillus pumilus m27 and bacillus subtilis h1584 based on the 16s rrna gene sequence homology. the weight loss of polyethylene was 1%, 1.5% and 1.75% after 30 days of incubation with the m16, m27 and h1584 i ...201324210946
synthesis and biological evaluation of bis-imidazolidineiminothiones: a comparative study.a series of 15 novel symmetrical and non-symmetrical bis-imidazolidineiminothiones (6a-g, 7a-e, 8a,b, and 9) with various substituents at n-(1) (p-tolyl, p-methoxyphenyl, p-ethoxyphenyl, p-chlorophenyl, p-bromophenyl, p-iodophenyl, and 3,5-dichlorophenyl) and different linkers between the n-(3) atoms [4,4'-oxybis(4,1-phenylene), 2,2'-dimethoxybiphenyl, and (1,3,3-trimethylcyclohexyl)methyl)] were prepared in 65-75% yields from substituted n-arylcyanothioformanilides and various bis-isocyanates. ...201323836516
an icebs1-like element may be associated with the extreme radiation and desiccation resistance of bacillus pumilus safr-032 spores.comparisons of the genomes of bacillus pumilus safr-032 and the closely related type strain, b. pumilus atcc7061(t), exposed an extended region of non-homologous genes. a detailed examination of this region revealed the presence of an icebs1-like integrative conjugative element in safr-032. a similar element was subsequently located elsewhere in the atcc7061(t) genome. a detailed comparison of these elements and the icebs1 of b. subtilis revealed extremely rapid flux in gene content, genome orga ...201323812891
expression and biochemical characterization of cold-adapted lipases from antarctic bacillus pumilus strains.two lipase genes (bpl1 and bpl3) from antarctic bacillus pumilus strains were expressed in bacillus subtilis. both recombinant lipases bpl1 and bpl2 were secreted to the culture medium and their activities reached 3.5 u/ml and 5.0 u/ml, respectively. their molecular masses apparent using sds-page were 23 kda for bpl1 and 19 kda for bpl3. both lipases were purified to homogeneity using ammonium sulfate precipitation and hitrap sp ff column and superose 12 column chromatographies. the final specif ...201323770563
utilization of low-pressure plasma to inactivate bacterial spores on stainless steel screws.a special focus area of planetary protection is the monitoring, control, and reduction of microbial contaminations that are detected on spacecraft components and hardware during and after assembly. in this study, wild-type spores of bacillus pumilus safr-032 (a persistent spacecraft assembly facility isolate) and the laboratory model organism b. subtilis 168 were used to study the effects of low-pressure plasma, with hydrogen alone and in combination with oxygen and evaporated hydrogen peroxide ...201323768085
reducing the radiation sterilization dose improves mechanical and biological quality while retaining sterility assurance levels of bone allografts.bone allografts carry a risk of infection, so terminal sterilization by gamma irradiation at 25kgy is recommended; but is deleterious to bone quality. contemporary bone banking significantly reduces initial allograft bioburden, questioning the need to sterilize at 25kgy.201323912050
genomic characterization of six novel bacillus pumilus bacteriophages.twenty-eight bacteriophages infecting the local host bacillus pumilus bl-8 were isolated, purified, and characterized. nine genomes were sequenced, of which six were annotated and are the first of this host submitted to the public record. the 28 phages were divided into two groups by sequence and morphological similarity, yielding 27 cluster bpa phages and 1 cluster bpb phage, which is a bl-8 prophage. most of the bpa phages have a host range restricted to distantly related strains, b. pumilus a ...201323906709
enhanced phytotransformation of navy blue rx dye by petunia grandiflora juss. with augmentation of rhizospheric bacillus pumilus strain pgj and subsequent toxicity analysis.this study reveals the beneficial synergistic phytoremediation potential of petunia grandiflora juss. with its rhizospheric bacterial isolate bacillus pumilus strain pgj to decolorize reactive navy blue rx (nbrx) dye by their active enzymatic machinery. in vitro cultures of p. grandiflora and b. pumilus gave 80.01% and 76.80% while their consortium decolorized nbrx up to 96.86% within 36 h. significant induction in the enzyme activities of lignin peroxidase (207%), tyrosinase (133%), laccase (16 ...201323743429
[bacillus pumilus strains with inactivated genes for extracellular serine proteinases]. 201323718049
isolation and characterization of lignin-degrading bacteria from rainforest soils.the deconstruction of lignin to enhance the release of fermentable sugars from plant cell walls presents a challenge for biofuels production from lignocellulosic biomass. the discovery of novel lignin-degrading enzymes from bacteria could provide advantages over fungal enzymes in terms of their production and relative ease of protein engineering. in this study, 140 bacterial strains isolated from soils of a biodiversity-rich rainforest in peru were screened based on their oxidative activity on a ...201323297115
biodiversity of aerobic endospore-forming bacterial species occurring in yanyanku and ikpiru, fermented seeds of hibiscus sabdariffa used to produce food condiments in benin.yanyanku and ikpiru made by the fermentation of malcavene bean (hibiscus sabdariffa) are used as functional additives for parkia biglobosa seed fermentations in benin. a total of 355 aerobic endospore-forming bacteria (aefb) isolated from yanyanku and ikpiru produced in northern and southern benin were identified using phenotypic and genotypic methods, including gtg5-pcr, m13-pcr, 16s rrna, gyra and gyrb gene sequencing. generally, the same 5-6 species of the genus bacillus predominated: bacillu ...201323571124
structural and functional insight into an unexpectedly selective n-methyltransferase involved in plantazolicin biosynthesis.plantazolicin (pzn), a polyheterocyclic, n(α),n(α)-dimethylarginine-containing antibiotic, harbors remarkably specific bactericidal activity toward strains of bacillus anthracis, the causative agent of anthrax. previous studies demonstrated that genetic deletion of the s-adenosyl-l-methionine-dependent methyltransferase from the pzn biosynthetic gene cluster results in the formation of desmethylpzn, which is devoid of antibiotic activity. here we describe the in vitro reconstitution, mutational ...201323878226
role of mechanical vs. chemical action in the removal of adherent bacillus spores during cip procedures.this study was designed to evaluate the respective roles of mechanical and chemical effects on the removal of bacillus spores during cleaning-in-place. this analysis was performed on 12 strains belonging to the bacillus cereus group (b. cereus, bacillus anthracis, bacillus thuringiensis) or to less related bacillus species (bacillus pumilus, bacillus licheniformis, bacillus sporothermodurans, bacillus subtilis). adherent spores were subjected to rinsing-in-place (mechanical action) and cleaning- ...201323200646
sporicidal efficacy of ph-adjusted bleach for control of bioburden on production facility surfaces.ph-adjusted bleach was one of the agents used to disinfect contaminated public buildings in the usa following the 2001 bioterrorist attack with bacillus anthracis spores. a usepa fact sheet describes the preparation of ph-adjusted bleach by combining diluted sodium hypochlorite (naocl) with a controlled amount of 5 % acetic acid. this paper reports a modification of this procedure to qualify the use of ph-adjusted bleach for routine disinfection of cleanroom surfaces in pharmaceutical manufactur ...201323532317
disruption of microbial biofilms by an extracellular protein isolated from epibiotic tropical marine strain of bacillus licheniformis.marine epibiotic bacteria produce bioactive compounds effective against microbial biofilms. the study examines antibiofilm ability of a protein obtained from a tropical marine strain of bacillus licheniformis d1.201323691235
induced phytoremediation of metals from fly ash mediated by plant growth promoting rhizobacteria.a study was carried out to observe the impact of a consortium of bacteria isolated from the fly ash on the metal accumulation by t. latifolia. when a consortium of bacteria bacillus endophyticus nbrft4 (mtcc 9021), paenibacillus macerans nbrfts (mtcc 8912) and bacillus pumilus nbrft9 (mtcc 8913) was bioaugmented into the rhizosphere of t. latifolia, it enhanced the metal concentration in root, stem and leaves of the plants through increased bioavailability of metals fe, cd, pb, cr, ni, cu and zn ...201324640248
evaluation of bacterial flora during the ripening of kedong sufu, a typical chinese traditional bacteria-fermented soybean product.kedong sufu is a typical bacteria-fermented sufu in china. isolation and identification of the autochthonous bacteria involved would allow the design of specific starters for this speciality. the purpose of the present study was to evaluate the bacterial flora during the ripening of kedong sufu using polymerase chain reaction denaturing gradient gel electrophoresis (pcr-dgge) and culturing.201323400969
activation of bacillus spores at moderately elevated temperatures (30-33 °c).the time/temperature profiles experienced by spores on the track from their natural sporulation environment to consumable food products may be highly diverse. temperature has been documented as an important factor that may activate spores, i.e. potentiates spores to germinate. there is, however, limited knowledge about the relationship between the expected temperature history and the subsequent germination characteristics of bacterial spores. we show here that the germination rate of five differ ...201323132276
rapid identification of dairy mesophilic and thermophilic sporeforming bacteria using dna high resolution melt analysis of variable 16s rdna regions.due to their ubiquity in the environment and ability to survive heating processes, sporeforming bacteria are commonly found in foods. this can lead to product spoilage if spores are present in sufficient numbers and where storage conditions favour spore germination and growth. a rapid method to identify the major aerobic sporeforming groups in dairy products, including bacillus licheniformis group, bacillus subtilis group, bacillus pumilus group, bacillus megaterium, bacillus cereus group, geoba ...201323743474
screening of solvent dependent antibacterial activity of prunus domestica.fruit of prunus domestica was extracted in ethanol. the ethanol extract was further extracted with two solvents ethyl acetate and chloroform. the crude ethanol extract and two fractions (ethyl acetate and chloroform) were screened for their antibacterial activity using the agar well diffusion method .they were tested against nine bacteria; five gram positive bacteria (staphylococcus aureus, streptococcuc intermedius, bacillus cereus, bacillus pumilus) and four gram negative bacteria (eschrichia ...201323455215
cloning and expression of dnak gene from bacillus pumilus of hot water spring origin.a set of thermotolerant strains isolated from hot springs of manikaran and bakreshwar (india) were selected with an aim to isolate dnak gene which encodes dnak protein. the gene dnak along with its flanking region was successfully amplified from 5 different strains (4 from bakreshwar and one from manikaran). restriction fragment length polymorphism (rflp) revealed that amplicons were almost identical in sequence. the dnak gene from one representative, bacillus pumilus strain b3 isolated from bak ...201327275408
effects of ensiling fermentation and aerobic deterioration on the bacterial community in italian ryegrass, guinea grass, and whole-crop maize silages stored at high moisture content.the effects of storage period and aerobic deterioration on the bacterial community were examined in italian ryegrass (ir), guinea grass (gg), and whole-crop maize (wm) silages. direct-cut forages were stored in a laboratory silo for 3, 7, 14, 28, 56, and 120 d without any additives; live counts, content of fermentation products, and characteristics of the bacterial community were determined. 2,3-butanediol, acetic acid, and lactic acid were the dominant fermentation products in the ir, gg, and w ...201325049913
cota, a multicopper oxidase from bacillus pumilus wh4, exhibits manganese-oxidase activity.multicopper oxidases (mcos) are a family of enzymes that use copper ions as cofactors to oxidize various substrates. previous research has demonstrated that several mcos such as mnxg, mofa and moxa can act as putative mn(ii) oxidases. meanwhile, the endospore coat protein cota from bacillus species has been confirmed as a typical mco. to study the relationship between cota and the mn(ii) oxidation, the cota gene from a highly active mn(ii)-oxidizing strain bacillus pumilus wh4 was cloned and ove ...201323577125
isolation and identification of bacterial endophytes from pharmaceutical agarwood-producing aquilaria species.resins and gums are used in traditional medicine and do have potential applications in pharmacy and medicine. agarwood is the fragrant resinous wood, which is an important commodity from aquilaria species and has been used as a sedative, analgesic, and digestive in traditional medicine. endophytic bacteria are potentially important in producing pharmaceutical compounds found in the plants. hence, it was important to understand which types of endophytic bacteria are associated with pharmaceutical ...201323798890
isr meets sar outside: additive action of the endophyte bacillus pumilus inr7 and the chemical inducer, benzothiadiazole, on induced resistance against bacterial spot in field-grown pepper.induced resistance has been recognized as an attractive tool for plant disease management in modern agriculture. during the last two decades, studies on chemically- and biologically elicited induced resistance have revealed previously unknown features of the plant defense response including defense priming. as a biological trigger for induced resistance, plant growth-promoting rhizobacteria (pgpr) are a group of root-associated bacteria that can reduce plant disease severity and incidence, and a ...201323717313
candidate genes that may be responsible for the unusual resistances exhibited by bacillus pumilus safr-032 spores.the spores of several bacillus species, including bacillus pumilus safr-032 and b. safensis fo-36b, which were isolated from the spacecraft assembly facility at nasa's jet propulsion laboratory, are unusually resistant to uv radiation and hydrogen peroxide. in order to identify candidate genes that might be associated with these resistances, the whole genome of b. pumilus safr-032, and the draft genome of b. safensis fo-36b were compared in detail with the very closely related type strain b. pum ...201323799069
efficacy of using harmless bacillus endospores to estimate the inactivation of cryptosporidium parvum oocysts in water.the need to use complex in vitro cell culture, expensive equipment, and highly-trained technicians that are available only to specialist laboratories has significantly limited studies assessing the potential of pulsed uv light (puv) to inactivate the waterborne parasite cryptosporidium parvum in drinking water. this constitutes the first study to report on the use of different non-pathogenic bacillus endospores as potential surrogate organisms to indicate the puv inactivation performance of a c. ...201323145570
exiguobacterium oxidotolerans, a halotolerant plant growth promoting rhizobacteria, improves yield and content of secondary metabolites in bacopa monnieri (l.) pennell under primary and secondary salt stress.brahmi (bacopa monnieri), an integral component of indian ayurvedic medicine system, is facing a threat of extinction owing to the depletion of its natural populations. the present study investigates the prospective of exploitation of halotolerant plant growth promoting rhizobacteria (pgpr) in utilising the salt stressed soils for cultivation of b. monnieri. the effects of two salt tolerant pgpr, bacillus pumilus (str2) and exiguobacterium oxidotolerans (str36) on the growth and content of bacos ...201323085953
improving genetic immobilization of a cellulase on yeast cell surface for bioethanol production using cellulose.in this study, saccharomyces cerevisiae was genetically engineered to harbor the capability of utilizing celluloses for bioethanol production by displaying active cellulolytic enzymes on the cell surface. an endo-1,4-β-glucanase gene egx was cloned from bacillus pumilus c-9 and its expression products, the egx cellulases, were displayed on the cell surface of s. cerevisiae by fusing egx with aga2 that encodes the binding subunit of the s. cerevisiae cell wall protein α-agglutinin. to achieve hig ...201322915066
pigmentation and sporulation are alternative cell fates in bacillus pumilus sf214.bacillus pumilus sf214 is a spore forming bacterium, isolated from a marine sample, able to produce a matrix and a orange-red, water soluble pigment. pigmentation is strictly regulated and high pigment production was observed during the late stationary growth phase in a minimal medium and at growth temperatures lower than the optimum. only a subpopulation of stationary phase cells produced the pigment, indicating that the stationary culture contains a heterogeneous cell population and that pigme ...201323634224
optimization of chitin extraction from shrimp waste with bacillus pumilus a1 using response surface methodology.chitin extraction from shrimp shells by biological treatment, using the bacilli bacillus pumilus a1, is a non-polluting method and offers the opportunity to preserve the exceptional qualities of chitin and its derivatives. however, the major disadvantage of the fermentative way is the low efficiency of demineralization and deproteinization. the aim of this study is to improve the yield of extraction which depends on many factors, such as the medium composition and the physical parameters. in ord ...201323831901
[influence of bacteria of bacillus genus on the causative agent of bacterial cancer of tomatoes].it has been shown that bacteria of the genus bacillus inhibited the development of infection caused by clavibacter michiganensis subsp. michiganensis, in tomatoes. pre-sowing seed treatment with suspensions of bacillus subtilis imv b-7023 and bacillus pumilus 3 enhanced resistance of plants to bacterial disease of cancer, probably due to the synthesis of biologically active substances with antimicrobial properties by these bacteria. of the two strains of bacillus, differing by antagonist propert ...201323120989
high-antibacterial activity of urtica spp. seed extracts on food and plant pathogenic bacteria.the aim of this study was to comparatively evaluate antibacterial activities of methanol (metoh) and aqueous (dw) leaf (l), root (r) and seed (s) extracts of urtica dioica l. (ud; stinging nettle) and urtica pilulifera l. (up; roman nettle) on both food- and plant-borne pathogens, with total phenolic contents and dpph radical scavenging activities (drsa). metoh extracts of leaves and roots of u. dioica had the highest drsa. extracts with high antibacterial activity were in the order up-lmetoh (1 ...201323067263
highly active β-xylosidases of glycoside hydrolase family 43 operating on natural and artificial substrates.the hemicellulose xylan constitutes a major portion of plant biomass, a renewable feedstock available for conversion to biofuels and other bioproducts. β-xylosidase operates in the deconstruction of the polysaccharide to fermentable sugars. glycoside hydrolase family 43 is recognized as a source of highly active β-xylosidases, some of which could have practical applications. the biochemical details of four gh43 β-xylosidases (those from alkaliphilus metalliredigens qymf, bacillus pumilus, bacill ...201323053115
the supernatant of bacillus pumilus sqr-n43 has antifungal activity towards rhizoctonia solani.for clarification of the antagonistic mechanism of bacillus pumilus sqr-n43 (n43) against rhizoctonia solani q1, production of antibiotics by n43 was determined, and the effect of the antibiotics on the pathogen mycelium was microscopically observed. further more, the control efficiencies of the antifungal compounds on damping-off disease were investigated. the results obtained are listed as follows: n43 produced antibiotic substances towards r. solani q1 at logarithmic growth phase. the antibio ...201323417338
development of a high-efficient transformation system of bacillus pumilus strain dx01 to facilitate gene isolation via gfp-tagged insertional mutagenesis and visualize bacterial colonization of rice roots.a tn5 transposition vector, pmod-tet-egfp, was constructed and used for the random insertional mutagenesis of bacillus pumilus. various parameters were investigated to increase the transformation efficiency b. pumilus dx01 via tn5 transposition complexes (transposome): bacterial growth phase, type of electroporation buffer, electric field strength, and recovery medium. transformation efficiency was up to 3 × 10(4) transformants/μg of dna under the optimized electroporation conditions, and a tota ...201323334949
improved activity and thermostability of bacillus pumilus lipase by directed evolution.to improve enzymatic activity of bacillus pumilus lipases, dna shuffling was applied to two lipase genes from local b. pumilus isolates. using a high-throughput activity assay, the mutant with highest activity was selected. this chimeric mutant (l3-3), carrying two crossover positions and three point mutations, has a specific activity 6.4 and 8.2 times higher than the two parent enzymes. the mutant also is more tolerant to various detergents and organic solvents, and has a 9 times longer half-li ...201323313890
biobleaching application of cellulase poor and alkali stable xylanase from bacillus pumilus sv-85s.the potential of extracellular alkali stable and thermo tolerant xylanase produced by bacilluspumilus sv-85s through solid state fermentation was investigated in pulp bleaching in association with conventional bleaching using chlorine and chlorine dioxide. the biobleaching of kraft pulp with xylanase was the most effective at an enzyme dose of 10 iu/g oven dried pulp, ph 9.0 and 120 min incubation at 55 °c. under the optimized conditions, xylanase pretreatment reduced kappa number by 1.6 points ...201328324585
isolation and characterization of indigenous endophytic bacteria associated with leaves of switchgrass (panicum virgatum l.) cultivars.to isolate and characterize indigenous bacterial endophytes from cultivars of switchgrass and study their antimicrobial and growth promoting potential.201323190162
wool-waste valorization: production of protein hydrolysate with high antioxidative potential by fermentation with a new keratinolytic bacterium, bacillus pumilus a1.wool, a recalcitrant waste mainly composed of keratin, constituted a serious problem for the environment and was not effectively valorized. this study reported the optimization of wool-waste biodegradation by a new keratinolytic bacterium bacillus pumilus a1. the in vitro digestibility and the antioxidant potential of wool protein hydrolysate (wph) were also investigated.201323663724
isolation and biochemical characterization of bacillus pumilus lipases from the antarctic.lipase-producing bacterial strains were isolated from antarctic soil samples using the tricaprylin agar plate method. seven strains with relatively strong lipase activities were selected. all of them turned out to be bacillus pumilus strains by the 16s rrna gene sequence analysis. their corresponding lipase genes were cloned, sequenced, and compared. finally, three different bacillus pumilus lipases (bpl1, bpl2, and bpl3) were chosen. their amino acid sequence identities were in the range of 92- ...201323648856
generation of biologically contained, readily transformable, and genetically manageable mutants of the biotechnologically important bacillus pumilus.bacillus pumilus mutants were generated by targeted deletion of a set of genes eventually facilitating genetic handling and assuring biological containment. the well-defined and stable mutants do not form functional endospores due to the deletion of yqfd, an essential sporulation gene; they are affected in dna repair, as δuvrba rendered them uv hypersensitive and, thus, biologically contained; they are deficient for the uracil phosphoribosyl-transferase (δupp), allowing for 5-fluorouracil-based ...201323644770
draft genome sequence of bacillus pumilus ccma-560, isolated from an oil-contaminated mangrove swamp.bacillus pumilus strain ccma-560 was isolated from an oil-contaminated mangrove swamp and was shown to produce biosurfactants. the strain appears to be capable of degrading some plant cell wall-related compounds, including hemicelluose and pectin. genes for biopolymer export and polysaccharide intercellular adhesin synthesis were also annotated.201324029758
studies on degradation efficiency of polycaprolactone by a naturally-occurring bacterium.we report a strain of bacillus, isolated from the rhizosphere of the mangrove sesuvium portulacastrum, that degrades polycaprolactone (pcl) on timescales that are a factor of three shorter than hitherto reported, with complete degradation in only 20 days. the bacterium has been identified as bacillus pumilus by means of 16s rrna gene sequencing and fame analysis; it secretes proteases and lipases and its 'de-polymerase' activity is evident by the zone of clearing in emulsified pcl. it is an aero ...201324006806
a possible mechanism of action of plant growth-promoting rhizobacteria (pgpr) strain bacillus pumilus wp8 via regulation of soil bacterial community structure.according to the traditional view, establishment and maintenance of critical population densities in the rhizosphere was the premise of pgpr to exert growth-promoting effects. in light of the facts that soil bacterial community structures can be changed by some pgpr strains including bacillus pumilus wp8, we hypothesize that regulation of soil bacterial community structure is one of the plant growth-promoting mechanisms of b. pumilus wp8, rather than depending on high-density cells in soil. in t ...201324005176
novel method utilizing microbial treatment for cleaner production of diosgenin from dioscorea zingiberensis c.h. wright (dzw).a novel method utilizing microbial treatment for cleaner production of diosgenin from dioscorea zingiberensis c.h. wright (dzw) was presented. a new bacillus pumilus hr19, which has the great ability to secrete pectinase, was screened and applied in the microbial treatment. low-pressure steam expansion pretreatment (lsep) was employed in advance to assist microbial treatment efficiently in releasing saponins, which are the precursors of diosgenin. compared with the traditional process of acid hy ...201323973974
an assay for functional xylose transporters in saccharomyces cerevisiae.it has been considered that more efficient uptake of xylose could promote increased xylose metabolic capacity of several microorganisms. in this study, an assay to screen xylose transporters was established in the saccharomyces cerevisiae strain, which expresses the xylosidase gene of bacillus pumilus intracellularly. the absorbed xylose analog p-nitrophenyl-β-d-xylopyranoside (pnpx) rapidly hydrolyzed to p-nitrophenol (pnp), which displayed a yellow tint when exposed to xylosidase in vivo. the ...201323928049
improving the chitinolytic activity of bacillus pumilus sg2 by random mutagenesis.bacillus pumilus sg2, a halotolerant strain, expresses two major chitinases designated chis and chil that were induced by chitin and secreted into the supernatant. the present work aimed to obtain a mutant with higher chitinolytic activity through mutagenesis of bacillus pumilus sg2 using a combination of uv irradiation and nitrous acid treatment. following mutagenesis and screening on chitin agar and subsequent formation of halos, the mutated strains were examined for degradation of chitin unde ...201323867702
bioinspired inimitable cadmium telluride quantum dots for bioimaging purposes.synthesis of quantum nanoparticles of specific size, shape and composition are an aspect important in nanotechnology research. although these nanostructures are routinely synthesized by chemical routes, the use of microorganisms has emerged as a promising option. the synthesis of cadmium telluride (cdte) quantum dots by two hitherto unreported marine bacteria (bacillus pumilus and serratia marcescens) is reported here. ultraviolet-visible (uv-vis) spectroscopy indicated the synthesis of cdte nan ...201323862414
[properties of bacillus pumilus subtilisin like proteinase secreted from recombinant strain on different growth stages].bacillus pumilus 3-19 glutamylendopeptidase has been isolated from culture liquid of bacillus subtilis recombinant strain on different growth stages: growth retardation (early enzyme) and stationary phase (late enzyme). the effect of purified proteinase of different growth stages on insulin beta-chain, protein and oligopeptide substrates has been studied. comparative study of physicochemical properties of early and late proteinases was carried out. two protein fractions were different in catalyt ...201323844506
covalent immobilization of xylanase produced from bacillus pumilus sv-85s on electrospun polymethyl methacrylate nanofiber membrane.polymethyl methacrylate (pmma) nanofiber membrane (nfm) was synthesized by an electrospinning technique. these membranes were utilized as a support for immobilization of xylanase enzyme to study its ph stability, thermal stability, and reusability. the morphology of aligned nfm was studied by optical microscopy and scanning electron microscopy. the pmma nfm was functionalized with phenylenediamine and activated with glutaraldehyde to yield an aldehyde group on its surface for covalent immobiliza ...201323586605
streptomyces lunalinharesii strain 235 shows the potential to inhibit bacteria involved in biocorrosion processes.four actinomycete strains previously isolated from brazilian soils were tested for their antimicrobial activity against bacillus pumilus lf-4 and desulfovibrio alaskensis ncimb 13491, bacteria that are well known to be involved in biofilm formation and biocorrosion. strain 235, belonging to the species streptomyces lunalinharesii, inhibited the growth of both bacteria. the antimicrobial activity was seen over a wide range of ph, and after treatment with several chemicals and heat but not with pr ...201323484107
thermostable keratinase from bacillus pumilus ks12: production, chitin crosslinking and degradation of sup35nm aggregates.production of thermostable keratinase from bacillus pumilus ks12 was enhanced up to seven fold by statistical methods. the enzyme was partially purified by ultrafiltration followed by thermal precipitation with purity of 3.2-fold and recovery of 89%. keratinase was immobilized using covalent method by crosslinking 2 mg protein (688 u/mg) onto 1g chitin activated with 2.5% (v/v) glutaraldehyde for 60 min. its comparative biochemical studies with that of free keratinase revealed the shift in optim ...201323425582
iodine from bacterial iodide oxidization by roseovarius spp. inhibits the growth of other bacteria.microbial activities in brine, seawater, or estuarine mud are involved in iodine cycle. to investigate the effects of the microbiologically induced iodine on other bacteria in the environment, a total of 13 bacteria that potentially participated in the iodide-oxidizing process were isolated from water or biofilm at a location containing 131 μg ml(-1) iodide. three distinct strains were further identified as roseovarius spp. based on 16 s rrna gene sequences after being distinguished by restricti ...201322526798
viability of phenanthrene biodegradation by an isolated bacterial consortium: optimization and scale-up.in the present work, biodegradation of phenanthrene by a bacterial consortium (lb2), isolated from lab-polluted soils has been investigated. the 16s rrna gene-based molecular analysis revealed that the bacterial consortium lb2 consisted of two strains showing a very high homology with staphylococcus warneri and bacillus pumilus. the optimization of phenanthrene degradation by the consortium lb2, using a central composite face-centered design was carried out taking into account three important pa ...201322717666
biological and genomic analysis of a pbsx-like defective phage induced from bacillus pumilus ab94180.defective prophages, which are found in the genomes of many bacteria, are unable to complete a viral replication cycle and propagate in their hosts as healthy prophages. they package random dna fragments derived from various sites of the host chromosome instead of their own genomes. in this study, we characterized a defective phage, pbp180, which was induced from bacillus pumilus ab94180 by treatment with mitomycin c. electron microscopy showed that the pbp180 particle has a head with a hexagona ...201424154951
a novel cephalosporin deacetylating acetyl xylan esterase from bacillus subtilis with high activity toward cephalosporin c and 7-aminocephalosporanic acid.a cephalosporin deacetylating acetyl xylan esterase was cloned from the genomic dna of bacillus subtilis cicc 20034 and functionally expressed in escherichia coli. its gene contained an open reading frame of 957 bp encoding 318 amino acids with a calculated mass of 35,607 da, and it displayed significant identity to acetyl xylan esterases from bacillus sp. 916, b. subtilis 168, and bacillus pumilus cect5072. the enzyme was a native homohexamer but a trimer under the condition of 1% sodium dodecy ...201423828600
genome sequence of the plant endophyte bacillus pumilus inr7, triggering induced systemic resistance in field crops.bacillus pumilus inr7 is an endophytic bacterium that has been commercialized as a biological control product against soilborne pathogens as well as foliar pathogens by direct antagonism and induction of systemic resistance. in the current study, we provide the genome sequence and a possible explanation of the function of strain inr7.201425359912
pgpr regulate caspase-like activity, programmed cell death, and antioxidant enzyme activity in paddy under salinity.the response of two root associated bacteria pseudomonas pseudoalcaligenes and bacillus pumilus were studied in the (salt-sensitive) rice gj17 cultivar to salinity under controlled environmental growth conditions for protection of plant from adverse effect of salinity. salinity affects the growth of salt-sensitive cultivar, but inoculation of plant growth promoting rhizobacteria (pgpr) reduces the harmful effect of salinity. the present study states that pgpr helps to reduce lipid peroxidation a ...201424757324
purification and characterization of [formula: see text]-mannanase from bacillus pumilus (m27) and its applications in some fruit juices.thermo alkaline mannanase was purified from the bacteria of bacillus pumilus (m27) using the techniques of ammonium sulphate precipitation, deae-sephadex ion exchange chromatography and sephacryl s200 gel filtration chromatography with 111-fold and 36 % yield. it was determined that the enzyme had 2 sub-units including 35 kda and 55 kda in gel filtration chromatography and sds-page electrophoresis systems. the optimum ph and temperature was determined as 8 and 60 °c, respectively. it was also no ...201426243955
a novel bacillus pumilus-related strain from tropical landfarm soil is capable of rapid dibenzothiophene degradation and biodesulfurization.the presence of organic sulfur-containing compounds in the environment is harmful to animals and human health. the combustion of these compounds in fossil fuels tends to release sulfur dioxide in the atmosphere, which leads to acid rain, corrosion, damage to crops, and an array of other problems. the process of biodesulfurization rationally exploits the ability of certain microorganisms in the removal of sulfur prior to fuel burning, without loss of calorific value. in this sense, we hypothesize ...201425293673
asterisulphoxide and asterisulphone: two new antibacterial and antifungal metabolites from the tunisian asteriscus maritimus (l.) less.two new sulphur-containing metabolites, asterisulphoxide 1 and asterisulphone 2, together with six known compounds, coniferaldehyde 4, 4-hydroxy-2-methoxybenzaldehyde 3, methylcaffeate 5, isobutyrate 10-isobutyryloxy-8,9-epoxythymyle 6, 8,9-dihydroxy-10-isobutyryloxythymol 7 and 8-hydroxy-9,14-diisobutyryloxythymol 8, were isolated from asteriscus maritimus roots. their structures were elucidated on the basis of spectroscopic evidence and comparison with authentic samples. compounds 1-8 were ass ...201424824823
growth characteristics and biofilm formation of various spoilage bacteria isolated from fresh produce.this study investigated the characteristics of spoilage bacteria isolated from fresh produce including growth at various temperatures, biofilm formation, cell hydrophobicity, and colony spreading. the number of spoilage bacteria present when stored at 35 °c was significantly greater than when stored at lower temperatures, and maximum population size was achieved after 10 h. however, bacillus pumilus, dickeya zeae, pectobacterium carotovorum subsp. carotovorum pcc21, and bacillus pumilus (rda-r) ...201425224561
isolation and characterization of genetic variability in bacteria with β-hemolytic and antifungal activity isolated from the rhizosphere of medicago truncatula plants.in the present study, we analyzed the frequency of hemolytic and antifungal activities in bacterial isolates from the rhizosphere of medicago truncatula plants. of the 2000 bacterial colonies, 96 showed β-hemolytic activities (frequency, 4.8 x 10(-2)). hemolytic isolates were analyzed for their genetic diversity by using random amplification of polymorphic dna, yielding 88 haplotypes. the similarity coefficient of nei and li showed a polymorphic diversity ranging from 0.3 to 1. additionally, 8 o ...201425062484
corrigendum to "prevalence of thermoduric bacteria and spores on 10 midwest dairy farms".thermoduric bacteria (tdb), including sporeformers and their spores, can be present in milk and dairy products even after pasteurization. they have the potential to adversely affect the quality and shelf life of products. the objectives of this study were to identify the origin and common species of heat-resistant bacteria occurring during summer and winter on midwest dairy farms. bulk tank milk samples were taken from 10 dairy farms located along the south dakota section of interstate 29, with ...201425610925
prevalence of thermoduric bacteria and spores on 10 midwest dairy farms.thermoduric bacteria (tdb), including sporeformers and their spores, can be present in milk and dairy products even after pasteurization. they have the potential to adversely affect the quality and shelf life of products. the objectives of this study were to identify the origin and common species of heat-resistant bacteria occurring during summer and winter on midwest dairy farms. bulk tank milk samples were taken from 10 dairy farms located along the south dakota section of interstate 29, with ...201425200773
biotransformation of arsenic by bacterial strains mediated by oxido-reductase enzyme system.the present study deals with the enzyme mediated biotransformation of arsenic in five arsenic tolerant strains (bacillus subtilis, bacillus megaterium, bacillus pumilus, paenibacillus macerans and escherichia coli). biotransformation ability of these isolates was evaluated by monitoring arsenite oxidase and arsenate reductase activity. results showed that arsenic oxidase activity was exclusively present in p. macerans and b. pumilus while b. subtilis, b. megaterium and e. coli strains showed pre ...201425535706
hard surface biocontrol in hospitals using microbial-based cleaning products.healthcare-associated infections (hais) are one of the most frequent complications occurring in healthcare facilities. contaminated environmental surfaces provide an important potential source for transmission of many healthcare-associated pathogens, thus indicating the need for new and sustainable strategies.201425259528
variations in the structure of airborne bacterial communities in a downwind area during an asian dust (kosa) event.asian dust (kosa) events transport airborne microorganisms that significantly impact biological ecosystems, human health, and ice-cloud formation in downwind areas. however, the composition and population dynamics of airborne bacteria have rarely been investigated in downwind areas during kosa events. in this study, air samplings were sequentially performed at the top of a 10-m high building within the kosa event arrival area (kanazawa city, japan) from may 1 to may 7, 2011, during a kosa event. ...201424815557
lna-modified isothermal oligonucleotide microarray for differentiating bacilli of similar origin.oligonucleotide microarray has been one of the most powerful tools in the 'post-genome era' for its high sensitivity, high throughput and parallel processing capability. to achieve high detection specificity, we fabricated an isothermal microarray using locked nucleic acid (lna)-modified oligonucleotide probes, since lna has demonstrated the advanced ability to enhance the binding affinity toward their complementary nucleotides. after designing the nucleotide sequences of these oligonucleotide p ...201425431409
phylogenetic and clonality analysis of bacillus pumilus isolates uncovered a highly heterogeneous population of different closely related species and clones.bacillus pumilus is a gram-positive bacterium with a wide range of attributed applications, namely as a plant growth promoting rhizobacteria (pgpr), animal, and human probiotic. however, a rare putative role in human diseases has been reported, namely in food poisoning or as anthrax-like cutaneous infectious agent. this species is difficult to distinguish from its closely related species on the basis of phenotypic or biochemical characteristics and 16s rrna gene sequences. in this study, the phy ...201425230950
comparison of identification systems for psychrotrophic bacteria isolated from raw bovine milk.psychrotrophic bacteria in raw milk produce heat-resistant extracellular proteases, resulting in spoilage and shelf-life reduction of ultrahigh temperature treated milk and milk products. controlling of these spoilage microbes requires rapid and reliable identification systems for screening of raw milk. this study aimed to compare commercial bacterial identification systems with a genetic method (considered as the 'gold standard' method) for the identification of heat-resistant protease producin ...201425113043
screening of marine bacteria with bacteriocin-like activities and probiotic potential for ornate spiny lobster (panulirus ornatus) juveniles.bacteriocins are ribosomally synthesized antimicrobial peptides, which have been found in diverse bacterial species of terrestrial origins and some from the sea. new bacteriocins with new characteristics, new origins and new applications are likely still awaiting discovery. the present study screened bacteria isolated from marine animals of interest to the aquaculture industry for antimicrobial and bacteriocin-like activities in order to uncover biodiversity of bacteriocin producers, and explore ...201424969424
bacillus invictae sp. nov., isolated from a health product.a gram-positive, rod-shaped, endospore-forming bacillus isolate, bi.(ffup1) (t), recovered in portugal from a health product was subjected to a polyphasic study and compared with the type strains of bacillus pumilus, bacillus safensis, bacillus altitudinis and bacillus xiamenensis, the phenotypically and genotypically most closely related species. acid production from cellobiose, d-glucose and d-mannose and absence of acid production from d-arabinose, erythritol, inositol, maltose, mannitol, raf ...201425171924
incidence, diversity and characteristics of spores of psychrotolerant spore formers in various repfeds produced in belgium.the major objectives of this study were to determine the incidence of psychrotolerant spore formers from repfeds marketed in belgium, and their diversity and characteristics. spore formers in general were found as spores on 38.3% of the food samples and in 85% food products types evaluated. 76% of the food samples containing spore formers had spores before enrichment. a total of 86 spore formers were isolated from the samples. 28 of 86 bacterial spore formers (32.6%) were capable of vegetative g ...201425084675
developing a new production host from a blueprint: bacillus pumilus as an industrial enzyme producer.since volatile and rising cost factors such as energy, raw materials and market competitiveness have a significant impact on the economic efficiency of biotechnological bulk productions, industrial processes need to be steadily improved and optimized. thereby the current production hosts can undergo various limitations. to overcome those limitations and in addition increase the diversity of available production hosts for future applications, we suggest a production strain blueprinting (psb) stra ...201424661794
the combined effect of pasteurization intensity, water activity, ph and incubation temperature on the survival and outgrowth of spores of bacillus cereus and bacillus pumilus in artificial media and food products.the objective of the study was to evaluate the combined effects of pasteurization intensity (no heat treatment and 10 min at 70, 80 and 90 °c), water activity (aw) (0.960-0.990), ph (5.5-7.0) and storage temperature (7 and 10 °c) on the survival and outgrowth of psychrotolerant spores of bacillus cereus ff119b and bacillus pumilus ff128a. the experiments were performed in both artificial media and a validation was performed on real food products (cream, béchamel sauce and mixed vegetable soup). ...201424801270
antibiofilm activity of biosurfactant producing coral associated bacteria isolated from gulf of mannar.coral associated bacteria (cab) (n = 22) isolated from the mucus of the coral acropora digitifera were screened for biosurfactants using classical screening methods; hemolysis test, lipase production, oil displacement, drop collapse test and emulsifying activity. six cab (u7, u9, u10, u13, u14, and u16) were found to produce biosurfactants and were identified by 16s ribosomal rna gene sequencing as providencia rettgeri, psychrobacter sp., bacillus flexus, bacillus anthracis, psychrobacter sp., a ...201425320434
enhancement of the immune response and protection against vibrio parahaemolyticus by indigenous probiotic bacillus strains in mud crab (scylla paramamosain).in a previous study, bacterial communities of the intestine in three populations of crabs (wild crabs, pond-raised healthy crabs and diseased crabs) were probed by culture-independent methods. in this study, we examined the intestinal communities of the crabs by bacterial cultivation with a variety of media. a total of 135 bacterial strains were isolated from three populations of mud crabs. the strains were screened for antagonistic activity against vibrio parahaemolyticus using an agar spot ass ...201425193866
bacillus xiamenensis sp. nov., isolated from intestinal tract contents of a flathead mullet (mugil cephalus).a taxonomic study was carried out on strain hyc-10(t), which was isolated from the intestinal tract contents of a flathead mullet, mugil cephalus, captured from the sea off xiamen island, china. the bacterium was observed to be gram positive, oxidase and catalase positive, rod shaped, and motile by subpolar flagella. the bacterium was found to grow at salinities of 0-12 % and at temperatures of 8-45 °c. the isolate was found to hydrolyze aesculin and gelatin, but was unable to reduce nitrate to ...201424158533
antibiofilm activity of bacillus pumilus sw9 against initial biofouling on microfiltration membranes.membrane biofouling, resulting from biofilm formation on the membrane, has become the main obstacle hindering wider application of membrane technology. initial biofouling proves to be crucial which involves early stages of microbial adhesion and biofilm formation. biological control of microbial attachment seems to be a promising strategy due to its high efficiency and eco-friendliness. the present study investigated the effects of a bacterium bacillus pumilus sw9 on controlling the initial foul ...201423715854
production of an alkaline protease using bacillus pumilus d3 without inactivation by sds, its characterization and purification.abstract in this study, protease-producing capacity of bacillus pumilus d3, isolated from hydrocarbon contaminated soil, was evaluated and optimized. optimum growing conditions for b. pumilus d3 in terms of protease production were determined as 1% optimum inoculum size, 35 °c temperature, 11 ph and 48 h incubation time, respectively. stability studies indicated that the mentioned protease was stable within the ph range of 7-10.5 and between 30 °c and 40 °c temperatures. surprisingly, the activi ...201423638694
novel extremely acidic lipases produced from bacillus species using oil substrates.the extremely acidophilic microorganisms bacillus pumilus and bacillus subtilis were isolated from soil collected from the commercial edible oil and fish oil extraction industry. optimization of conditions for acidic lipase production from b. pumilus and b. subtilis using palm oil and fish oil, respectively, was carried out using response surface methodology. the extremely acidic lipases, thermo-tolerant acidic lipase (tal) and acidic lipase (al), were produced by b. pumilus and b. subtilis, res ...201424185617
lipase production from a novel thermo-tolerant and extreme acidophile bacillus pumilus using palm oil as the substrate and treatment of palm oil-containing wastewater.the thermo-tolerant and extreme acidophilic microorganism bacillus pumilus was isolated from the soil collected from a commercial edible-oil extraction industry. optimisation of conditions for the lipase production was conducted using response surface methodology. the optimum conditions for obtaining the maximum activity (1,100 u/ml) of extremely acidic thermostable lipase were fermentation time, 96 h; ph, 1; temperature, 50 °c; and concentration of palm oil, 50 g/l. after purification, a 7.1-fo ...201424293300
characterization of cis-acting elements residing in the chitinase promoter of bacillus pumilus sg2.bacillus pumilus sg2 is a chitinolytic bacterium that produces two chitinases, namely chis and chil. the chis and chil genes are consecutively expressed under a common promoter. regulation of the chis and chil genes is under the control of carbon catabolite repression (ccr) in b. pumilus. this study aimed to investigate the cis-acting elements of the chitinase promoter. for this purpose, we transferred the chis gene along with its specific promoter to bacillus subtilis as a host. primer extensio ...201424293243
probing the crucial role of leu31 and thr33 of the bacillus pumilus cbs alkaline protease in substrate recognition and enzymatic depilation of animal hide.the sapb gene, encoding bacillus pumilus cbs protease, and seven mutated genes (sapb-l31i, sapb-t33s, sapb-n99y, sapb-l31i/t33s, sapb-l31i/n99y, sapb-t33s/n99y, and sapb-l31i/t33s/n99y) were overexpressed in protease-deficient bacillus subtilis db430 and purified to homogeneity. sapb-n99y and rsapb displayed the highest levels of keratinolytic activity, hydrolysis efficiency, and enzymatic depilation. interestingly, and at the semi-industrial scale, rsapb efficiently removed the hair of goat hid ...201425264614
bacterial enzymes effectively digest alzheimer's β-amyloid peptide.aggregated β-amyloid peptides play key roles in the development of alzheimer's disease, and recent evidence suggests that microbial particles, among others, can facilitate their polymerization. bacterial enzymes, however, have been proved to be beneficial in degrading pathological fibrillar structures in clinical settings, such as strepto-kinases in resolving blood-clots. the purpose of this study was to investigate the ability of bacterial substances to effectively hydrolyze β-amyloid peptides. ...201425262575
molecular characterization of a proteolysis-resistant lipase from bacillus pumilus sg2.proteolysis-resistant lipases can be well exploited by industrial processes which employ both lipase and protease as biocatalysts. a proteolysis resistant lipase from bacillus pumilus sg2 was isolated, purified and characterized earlier. the lipase was resistant to native and commercial proteases. in the present work, we have characterized the lip gene which encodes the proteolysis-resistant lipase from bacillus pumilus sg2. the parameters and structural details of lipase were analysed. the lip ...201425242920
immobilization of xylanase purified from bacillus pumilus vlk-1 and its application in enrichment of orange and grape juices.this study was conducted to evaluate the efficacy of purified free and immobilized xylanase in enrichment of fruit juices. extracellular xylanase produced from bacillus pumilus vlk-1 was purified to apparent homogeneity by 15.4-fold with 88.3 % recovery in a single step using cm-sephadex c-50. purified xylanase showed a single band on sds-polyacrylamide gel with a molecular mass of 22.0 kda. the purified enzyme was immobilized on glutaraldehyde-activated aluminum oxide pellets and the immobiliza ...201425190829
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