Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| bacillus pumilus laccase: a heat stable enzyme with a wide substrate spectrum. | laccases are multi-copper oxidases that catalyze the one electron oxidation of a broad range of compounds. laccase substrates include substituted phenols, arylamines and aromatic thiols. such compounds are activated by the enzyme to the corresponding radicals. owing to their broad substrate range laccases are considered to be versatile biocatalysts which are capable of oxidizing natural and non-natural industrial compounds, with water as sole by-product. | 2011 | 21266052 |
| molecular analysis of autochthonous microbiota along the digestive tract of juvenile grouper epinephelus coioides following probiotic bacillus pumilus administration. | aims: to evaluate the diversity of dominant autochthonous microbiota along the digestive tract of juvenile epinephelus coioides following the dietary administration of probiotic bacillus pumilus for 60 days. methods and results: polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) with subsequently sequencing analysis was used to assess the gut microbiota. generally similar dgge patterns were observed in the foregut, midgut and hindgut of e. coioides, while the similari ... | 2011 | 21294823 |
| purification and characterization of manganese-dependent alkaline serine protease from bacillus pumilus tms55. | the purification and characterization of a mn2+-dependent alkaline serine protease produced by bacillus pumilus tms55 were investigated. the enzyme was purified in three steps: concentrating the crude enzyme using ammonium sulfate precipitation, followed by gel filtration and cation-exchange chromatography. the purified protease had a molecular mass of approximately 35 kda, was highly active over a broad ph range of 7.0 to 12.0, and remained stable over a ph range of 7.5 to 11.5. the optimum tem ... | 2011 | 21301188 |
| a mixed-species microarray for identification of food spoilage bacilli. | failure of food preservation is frequently caused by thermostable spores of members of the bacillaceae family, which show a wide spectrum of resistance to cleaning and preservation treatments. we constructed and validated a mixed-species genotyping array for 6 bacillus species, including bacillus subtilis, bacillus licheniformis, bacillus pumilus, bacillus sporothermodurans, bacillus cereus and bacillus coagulans, and 4 geobacillus species, including geobacillus stearothermophilus, geobacillus t ... | 2010 | 21315980 |
| [screening, identification, and biocontrol effect of antagonistic bacteria against phytophthora capsici]. | a total of 98 isolates with antagonistic activity against phytophthora capsici were isolated from the rhizosphere soil of healthy pepper plants in the fields seriously infected by pepper phytophthora capsicit, and two strains named as hl-3 and lz-8 were screened, which had the characteristics of wide-spectrum antagonism and good growth under poor soil condition. the hl-3 and lz-8 were identified as paenibacillus polymyxa and bacillus pumilus, respectively, based on their morphological and bioche ... | 2010 | 21328956 |
| cloning, expression and characterization of glycoside hydrolase family 11 endoxylanase from bacillus pumilus ara. | an endoxylanase gene, xyna, was cloned from bacillus pumilus ara and expressed in escherichia coli. the open reading frame of the xyna gene was 687 bp encoding a signal peptide and a mature xylanase with a molecular mass of 23 kda. the enzyme was categorized as a glycosyl hydrolase family 11 member based on the sequence analysis of the putative catalytic domain. the recombinant xyna (bpu xyna) was purified to homogeneity by ni-nta and ion exchange chromatography on deae-sepharose ff. the enzyme ... | 2011 | 21369910 |
| assessment and characterization of heavy metal resistance in palk bay sediment bacteria. | the present study aimed at characterizing the heavy metal resistance and assessing the resistance pattern to multiple heavy metals (300 mmol l?¹) by palk bay sediment bacteria. from 46 isolates, 24 isolates showed resistance to more than eight heavy metals. among the 24 isolates s8-06 (bacillus arsenicus), s8-10 (bacillus pumilus), s8-14 (b. arsenicus), s6-01 (bacillus indicus), s6-04 (bacillus clausii), ss-06 (planococcus maritimus) and ss-08 (staphylococcus pasteuri) exhibited high resistance ... | 2011 | 21377723 |
| the crystal structure of the cephalosporin deacetylating enzyme acetyl xylan esterase bound to paraoxon explains the low sensitivity of this serine hydrolase to organophosphate inactivation. | organophosphorus insecticides and nerve agents irreversibly inhibit serine hydrolase superfamily enzymes. one enzyme of this superfamily, the industrially important (for +¦-lactam antibiotic synthesis) axe/cah (acetyl xylan esterase/cephalosporin acetyl hydrolase) from the biotechnologically valuable organism bacillus pumilus, exhibits low sensitivity to the organophosphate paraoxon (diethyl-p-nitrophenyl phosphate, also called paraoxon-ethyl), reflected in a high k(i) for it (~5 mm) and in a sl ... | 2011 | 21382014 |
| characterization and emulsifying property of a carbohydrate polymer produced by bacillus pumilus uw-02 isolated from waste water irrigated agricultural soil. | bacillus pumilus uw-02, an isolate from agricultural soil irrigated with waste water was found to produce a carbohydrate polymer in the form of extracellular polysaccharide (eps) in glucose mineral salts medium (gmsm). the recovery rates of eps by ion-exchange and gel filtration chromatography were around 63% and 90%, respectively. as evident from hplc and ft-ir analyses, the eps was found to be a heteropolymer consisting glucose, mannose, xylose, arabinose, and n-acetyl glucosamine as monomer u ... | 2011 | 21382404 |
| overexpression and characterization of thermostable chitinase from bacillus atrophaeus sc081 in escherichia coli. | the chitinase-producing strain sc081 was isolated from korean traditional soy sauce and identified as bacillus atrophaeus based on a phylogenetic analysis of the 16s rdna sequence and a phenotypic analysis. a gene encoding chitinase from b. atrophaeus sc081 was cloned in escherichia coli and was named scchi-1 (gq360078). the scchi-1 nucleotide sequences were composed of 1788 base pairs and 596 amino acids, which were 92.6, 89.6, 89.3, and 78.9% identical to those of bacillus subtilis (abg57262), ... | 2011 | 21429298 |
| xylanase production using agro-residue in solid-state fermentation from bacillus pumilus ash for biodelignification of wheat straw pulp. | two stage statistical design was used to optimize xylanase production from bacillus pumilus ash under solid-state fermentation. initially, plackett-burman designing (pb) was used for the selection of crucial production parameters. peptone, yeast extract, incubation time, moisture level and ph were found to be the crucial factors for the xylanase production. crucial variables were further processed through central composite designing (ccd) of response surface methodology (rsm) to maximize the xyl ... | 2011 | 21437760 |
| isolation and characterization of exopolysaccharide secreted by a toxic dinoflagellate, amphidinium carterae hulburt 1957 and its probable role in harmful algal blooms (habs). | extracellular polymeric substances (eps) produced by a toxic dinoflagellate amphidinium carterae hulburt 1957 was isolated and characterized. molecular masses of the eps were about 233 and 1,354 kda. spectral analyses by (1)h nuclear magnetic resonance and fourier transformed-infrared spectroscopy revealed the characteristic of the functional groups viz. primary amine, carboxyl, halide, and sulfate groups present in the eps. however, five elements (c, o, na, s, and ca) were detected by scanning ... | 2011 | 21503775 |
| silver nanoparticles tolerant bacteria from sewage environment. | silver nanoparticle (snp) is a threat to soil, water and human health. protection of environment from silver nanoparticles is a major concern. a sewage isolate, bacillus pumilus treated with snps showed similar growth kinetics to that without nanoparticles. a reduction in the amount of exopolysaccharides was observed after snps--b. pumilus culture supernatant interaction. the fourier transform infrared spectroscopy (ft-ir) peaks for the exopolysaccharides extracted from the bacterial culture sup ... | 2011 | 21517011 |
| enhancing jatropha oil extraction yield from the kernels assisted by a xylan-degrading bacterium to preserve protein structure. | we investigated the use of bacterial cells isolated from paddy crab for the extraction of oil from jatropha seed kernels in aqueous media while simultaneously preserving the protein structures of this protein-rich endosperm. a bacterial strain-which was marked as mb4 and identified by means of 16s rdna sequencing and physiological characterization as either bacillus pumilus or bacillus altitudinis-enhanced the extraction yield of jatropha oil. the incubation of an mb4 starter culture with prehea ... | 2011 | 21556918 |
| optimization of microwave-assisted fecl(3) pretreatment conditions of rice straw and utilization of trichoderma viride and bacillus pumilus for production of reducing sugars. | in this study, box-behnken design (bbd) and response surface methodology (rsm) were used to optimize microwave-assisted fecl(3) pretreatment conditions of rice straw with respect to fecl(3) concentration, microwave intensity, irradiation time and substrate concentration. when rice straw was pretreated at the optimal conditions of fecl(3) concentration, 0.14mol/l; microwave intensity, 160°c; irradiation time, 19min; substrate concentration, 109g/l; and inoculated with trichoderma viride and bacil ... | 2011 | 21561766 |
| rapd-based screening for spore-forming bacterial populations in uruguayan commercial powdered milk. | the occurrence of spore-forming bacteria in powdered milk is of concern to the dairy industry due to potential deleterious effects including those resulting from proteolytic and lipolytic activities. twenty-two powdered milk samples representative of spring and summer production obtained from uruguayan retail stores were analyzed for type and number of thermophilic and spore-forming bacterial species. bacillus licheniformis isolates were found to be the most prominent milk powder contaminant fol ... | 2011 | 21565415 |
| molecular cloning, sequence and structural analysis of dehairing mn(2+) dependent alkaline serine protease (maspt) of bacillus pumilus tms55. | leather industries release a large amount of pollution-causing chemicals which creates one of the major industrial pollutions. the development of enzyme based processes as a potent alternative to pollution-causing chemicals is useful to overcome this issue. proteases are enzymes which have extensive applications in leather processing and in several bioremediation processes due to their high alkaline protease activity and dehairing efficacy. in the present study, we report cloning, characterizati ... | 2011 | 21592076 |
| a novel compound from the marine bacterium bacillus pumilus s6-15 inhibits biofilm formation in gram-positive and gram-negative species. | biofilm formation is a critical problem in nosocomial infections and in the aquaculture industries and biofilms show high resistance to antibiotics. the aim of the present study was to reveal a novel anti-biofilm compound from marine bacteria against antibiotic resistant gram-positive and gram-negative biofilms. the bacterial extract (50 μg ml(-1)) of s6-01 (bacillus indicus = mtcc 5559) showed 80-90% biofilm inhibition against escherichia coli, shigella flexneri, proteus mirabilis and s6-15 (ba ... | 2011 | 21614700 |
| rice dehydrin k-segments have in vitro antibacterial activity. | dehydrins are groups of plant proteins that have been shown to response to various environmental stimuli such as dehydration, elevated salinity, and low temperature. however, their roles in plant defense against microbes have not been demonstrated. in an attempt to discover plant antimicrobial proteins, we have screened a rice cdna library and isolated several cdnas coding for dehydrins. protein extracts from escherichia coli expressing these cdnas were tested for their activity against gram-pos ... | 2011 | 21639844 |
| lysozyme as a barrier to growth of bacillus anthracis strain sterne in liquid egg white, milk and beef. | in this study, we investigated the role of lysozyme on the viability of bacillus cereus, bacillus subtilis, bacillus pumilus and bacillus anthracis (sterne) in egg white (ew), ground beef and milk. at 35 °c in ew, growth rates (gr) for b. cereus, b. subtilis, b. pumilus and b. anthracis were 0.005, -0.018, -0.028 and -0.029 od(600)/h, respectively. heat-treating ew at 55 and 60 °c reduced the inactivating effect of ew by 3.1 and 10.5-fold, respectively. addition of lysozyme (2 mg/ml) to 60 °c-tr ... | 2011 | 21645824 |
| comparative studies on lignin and polycyclic aromatic hydrocarbons degradation by basidiomycetes fungi. | a total of 130 wild basidiomycetes fungi were collected and identified. the polycyclic aromatic hydrocarbons (pahs) degradation by the potential phellinus sp., polyporus sulphureus (in liquid state fermentation (lsf), solid state fermentation (ssf), in soil) and lignin biodegradation were compared with those of a bacterial isolate and their corresponding cocultures. the pahs degradation was higher in lsf and the efficiency of the organisms declined in ssf and in soil treatment. phellinus sp. sho ... | 2011 | 21683591 |
| antimicrobial activity of essential oil and various extracts of fruits of greater cardamom. | greater cardamom (amomum subulatum roxb. zingiberaceae) commonly known as "bari ilaichi" is a well known plant used in ayurvedic and unani medicine. it has been used for the treatment of various diseases and disorders like gastric ulcer. therefore antimicrobial activity of petroleum ether, methanol and aqueous extracts from leaves and roots, essential oil and isolated vasicine from a. vasica were tested against various microorganisms. antimicrobial activity was done by disc diffusion method. the ... | 2010 | 21695005 |
| molecular phylogenetic diversity of bacillus community and its temporal-spatial distribution during the swine manure of composting. | in order to obtain the diversity and temporal-spatial distribution of bacillus community during the swine manure composting, we utilized traditional culture methods and the modern molecular biology techniques of polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) and -denaturing gradient gel electrophoresis (pcr-dgge). bacillus species were firstly isolated from the composting. based on temperature changes, the temporal-spatial characteristics of total culturable bacill ... | 2011 | 21701982 |
| anti-biofilm potential of a glycolipid surfactant produced by a tropical marine strain of serratia marcescens. | a tropical marine bacterium isolated from the hard coral, symphyllia sp. was identified as serratia marcescens on the basis of morphological, biochemical and 16s rdna analysis. the bacterium showed antimicrobial activity towards the pathogens candida albicans and pseudomonas aeruginosa and the marine biofouling bacterium bacillus pumilus. s. marcescens displayed biosurfactant activity as evidenced by drop collapse, blood hemolysis and surface tension reduction (52.0-27 mn m(-1)). the active comp ... | 2011 | 21707248 |
| characterization and crystal structure of the type iig restriction endonuclease rm.bpusi. | a type iig restriction endonuclease, rm.bpusi from bacillus pumilus, has been characterized and its x-ray crystal structure determined at 2.35+à resolution. the enzyme is comprised of an array of 5-folded domains that couple the enzyme's n-terminal endonuclease domain to its c-terminal target recognition and methylation activities. the rease domain contains a pd-x(15)-exk motif, is closely superimposable against the foki endonuclease domain, and coordinates a single metal ion. a helical bundle d ... | 2011 | 21724614 |
| biocontrol of rhizoctonia solani damping-off disease in cucumber with bacillus pumilus sqr-n43. | biological control is an efficient and environmentally friendly way to prevent damping-off disease. micrographs were used to investigate the ability of bacillus pumilus (b. pumilus) sqr-n43 to control rhizoctonia solani (r. solani) q1 in cucumbers. the root colonization ability of b. pumilus sqr-n43 was analyzed in vivo with a green fluorescent protein (gfp) tag. a pot experiment was performed to assess the in vivo disease-control efficiency of b. pumilus sqr-n43 and its bio-organic fertilizer. ... | 2011 | 21775112 |
| sterilization of biological pathogens using supercritical fluid carbon dioxide containing water and hydrogen peroxide. | novel noninvasive techniques for the removal of biological contaminants to generate clean or sterile materials are in demand by the medical, pharmaceutical and food industries. the sterilization method described here uses supercritical fluid carbon dioxide (sf-co(2)) containing 3.3% water and 0.1% hydrogen peroxide (v/v/v) to achieve a minimum 6 log viability reduction of all tested microbial species, including vegetative cells, spores and biofilms. the sterilization method employs moderate pres ... | 2011 | 21787810 |
| functional analysis of the sortase yhcs in bacillus subtilis. | sortases of gram-positive bacteria catalyze the covalent c-terminal anchoring of proteins to the cell wall. bacillus subtilis, a well known host organism for protein production, contains two putative sortases named yhcs and ywpe. the present studies were aimed at investigating the possible sortase function of these proteins in b. subtilis. proteomics analyses revealed that sortase-mutant cells released elevated levels of the putative sortase substrate yfkn into the culture medium upon phosphate- ... | 2011 | 21800427 |
| Antibacterial activity of different degree of hydrolysis of palm kernel expeller peptides against spore-forming and non-spore-forming bacteria. | The goal of this study was to determine inhibitory effect of palm kernel expeller (PKE) peptides of different degree of hydrolysis (DH %) against spore-forming bacteria Bacillus cereus, Bacillus circulans, Bacillus coagulans, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus stearothermophillus, Bacillus subtilis, Bacillus thuringiensis, Clostridium perfringens; and non-spore-forming bacteria Escherichia coli, Lisinibacillus sphaericus, Listeria monocytogenes, Pseudomonas a ... | 2011 | 21848644 |
| biodegradation of soil-applied pesticides by selected strains of plant growth-promoting rhizobacteria (pgpr) and their effects on bacterial growth. | a laboratory study was conducted to investigate the influence of four pgpr strains on the degradation of five soil applied pesticides and their effects on bacterial growth. interactions of bacillus subtilis gb03, bacillus subtilis fzb24, bacillus amyloliquefaciens in937a and bacillus pumilus se34 with two concentrations of acibenzolar-s-methyl, metribuzin, napropamide, propamocarb hydrochloride and thiamethoxam in liquid culture and soil microcosm were studied. the degradation of acibenzolar-s-m ... | 2011 | 21870159 |
| distinct differentiation of closely related species of bacillus subtilis group with industrial importance. | pcr amplification of 16s rrna gene by universal primers followed by restriction fragment length polymorphism analysis using rsai, cfoi and hinfi endonucleases, distinctly differentiated closely related bacillus amyloliquefaciens, bacillus licheniformis and bacillus pumilus from bacillus subtilis sensu stricto. this simple, economical, rapid and reliable protocol could be an alternative to misleading phenotype-based grouping of these closely related species. | 2011 | 21889958 |
| engineering ph-tolerant mutants of a cyanide dihydratase. | cyanide dihydratase is an enzyme in the nitrilase family capable of transforming cyanide to formate and ammonia. this reaction has been exploited for the bioremediation of cyanide in wastewater streams, but extending the ph operating range of the enzyme would improve its utility. in this work, we describe mutants of bacillus pumilus c1 cyanide dihydratase (cynd(pum)) with improved activity at higher ph. error-prone pcr was used to construct a library of cynd(pum) mutants, and a high-throughput s ... | 2011 | 21993481 |
| a catalase-peroxidase for oxidation of β-lactams to their (r)-sulfoxides. | in this communication we report for the first time a biocatalytic method for stereoselective oxidation of β-lactams, represented by penicillin-g, penicillin-v and cephalosporin-g to their (r)-sulfoxides. the method involves use of a bacterium, identified as bacillus pumilis as biocatalyst. the enzyme responsible for oxidase activity has been purified and characterized as catalase-peroxidase (katg). katg of b. pumilis is a heme containing protein showing characteristic heme spectra with soret pea ... | 2011 | 21996477 |
| phosphate-containing cell wall polymers of bacilli. | anionic phosphate-containing cell wall polymers of bacilli are represented by teichoic acids and poly(glycosyl 1-phosphates). different locations of phosphodiester bonds in the main chain of teichoic acids as well as the nature and combination of the constituent structural elements underlie their structural diversity. currently, the structures of teichoic acids of bacilli can be classified into three types, viz. poly(polyol phosphates) with glycerol or ribitol as the polyol; poly(glycosylpolyol ... | 2011 | 21999535 |
| Cutinolytic esterase activity of bacteria isolated from mixed-plant compost and characterization of a cutinase gene from Pseudomonas pseudoalcaligenes. | The objective of the current study was to examine cutinolytic esterase (i.e., cutinase) activity by pseudomonads and bacteria isolated from mixed-plant compost. Approximately 400 isolates representing 52 taxa recovered from mixed-plant compost using cuticle baits, along with 117 pseudomonad isolates obtained from a culture collection (i.e., non-compost habitats), were evaluated. The ability of isolates to degrade the synthetic cutin polycaprolactone (PCL) was initially measured. Isolates from 23 ... | 2011 | 22029433 |
| Genome sequence of Bacillus pumilus S-1, an efficient isoeugenol-utilizing producer for natural vanillin. | Bacillus pumilus S-1 is an efficient isoeugenol-utilizing producer of natural vanillin. The genome of B. pumilus S-1 contains the epoxide hydrolase and six candidate monooxygenases that make it possible to explore the mechanism involved in conversion of isoenguenol to vanillin in the B. pumilus strain. | 2011 | 22038964 |
| [screening of optimal signal peptide for heterologous xylanase secretion by bacillus subtilis]. | we searched optimal signal peptide for heterologous and exogenous secretion of xylanase in bacillus subtilis. | 2011 | 22043800 |
| A Rapid Microbioassay for Discovery of Antagonistic Bacteria for Phytophthora parasitica var. nicotianae. | A simple, rapid, small-scale microbioassay for infection of tobacco seedlings by Phytophthora parasitica var. nicotianae was developed here. This assay uses tobacco seedlings cultivated in Petri dishes for a standardized method for quantitation of initial zoospore inocula and high-throughput screening of antagonistic bacteria. Zoospore inocula between 102 to 105 spores per Petri dish were inoculated on 14-day-old tobacco seedlings for the susceptibility test. One thousand zoospores' treatment wa ... | 2011 | 22046969 |
| use of bacterial extracts to enhance amino acid breakdown in dry fermented sausages. | the effect of the intracellular cell-free extracts (icfes) of two bacterial strains (lactobacillus sakei go and bacillus pumilus) on the amino acid catabolism and the sensory properties of dry fermented sausages, was investigated. extracts were added to sausages alone or in combination with a protease, papain. amino acid breakdown was monitored by the changes in free amino acids, ammonia and amine content during the ripening process. a 15% decrease in the content of free amino acids was observed ... | 2006 | 22061560 |
| Application of lignocellulolytic enzymes produced under solid state cultivation conditions. | In this paper, cellulose from brown-rot fungus Fomitopsis sp. RCK2010, thermostable and alkalostable xylanase from Bacillus pumilus MK001 and laccase from Ganoderma sp. rckk-02 were evaluated for (i) saccharification of alkali pretreated rice straw and wheat straw, (ii) upgradation of chick feed and (iii) decolorization of dyes, respectively. The cellulose from brown-rot fungus resulted in a sugar release of 151.48 and 214.11mg/g, respectively, from rice straw and wheat straw, which was comparat ... | 2011 | 22067437 |
| metal complexes of diisopropylthiourea: synthesis, characterization and antibacterial studies. | co(ii), cu(ii), zn(ii) and fe(iii) complexes of diisopropylthiourea have been synthesized and characterized by elemental analyses, molar conductivity, magnetic susceptibility, ftir and electronic spectroscopy. the compounds are non-electrolytes in solution and spectroscopic data of the complexes are consistent with 4-coordinate geometry for the metal(ii) complexes and six coordinate octahedral for fe(iii) complex. the complexes were screened for their antibacterial activities against six bacteri ... | 2011 | 22072941 |
| First report of a bifunctional chitinase/lysozyme produced by Bacillus pumilus SG2. | Bacillus pumilus SG2 isolated from high salinity ecosystem in Iran produces two chitinases (ChiS and ChiL) and secretes them into the medium. In this study, chiS and chiL genes were cloned in pQE-30 expression vector and were expressed in the cytoplasm of Escherichia coli strain M15. The recombinant proteins were purified using Ni-NTA column. The optimum pH and optimum temperature for enzyme activity of ChiS were pH 6, 50°C; those of ChiL were pH 6.5, 40°C. The purified chitinases showed antifun ... | 2011 | 22112904 |
| evaluation of fluorescence in situ hybridization to detect encapsulated bacillus pumilus safr-032 spores released from poly(methylmethacrylate). | bacillus pumilus safr-032 spores originally isolated from jet propulsion laboratory spacecraft assembly facility clean room are extremely resistant to uv-radiation, h(2) o(2) , desiccation, chemical disinfections and starvation compared to spores of other bacillus species. the resistance of b. pumilus safr-032 spores to standard industrial clean room sterilization practices is not only a major concern for medical, pharmaceutical and food industries, but also a threat to extraterrestrial environm ... | 2011 | 22145981 |
| Induction of apoptosis and cell cycle arrest by Bis (2-ethylhexyl) phthalate produced by marine Bacillus pumilus MB 40. | Marine microorganisms represent a potential source for the production of biomedically useful compounds active against inflammation, cancer, diabetes, etc. Marine Bacillus pumilus MB 40 (GenBank accession no. HQ705771) isolated from deep sea water column (1000m depth) near Andaman and Nicobar islands produced a bioactive lead, Bis (2-ethylhexyl) phthalate (BEHP) with a molecular formula of C(6)H(4)(CO(2)C(8)H(17))(2) and a molecular ion at m/z 391 (M(+)). Anti proliferative effect of the isolated ... | 2011 | 22155658 |
| Two cases of severe sepsis caused by Bacillus pumilus in neonatal infants. | Bacillus pumilus is an environmental contaminant that has been rarely associated with clinical infections. Two cases of B. pumilus severe sepsis are described in two full-term neonates, one in a female infant without any predisposing factor and the other in a male infant in mechanical ventilation with intravenous catheter. In both cases, the microorganism was recovered from repeated blood cultures and was identified using biochemical and molecular assays. Both infants were successfully treated w ... | 2011 | 22174371 |
| the overexpression of the sapb of bacillus pumilus cbs and mutated sapb-l31i/t33s/n99y alkaline proteases in bacillus subtilis db430: new attractive properties for the mutant enzyme. | the sapb gene encoding for bacillus pumilus cbs protease (sapb) and the triple mutated sapb-l31i/t33s/n99y gene were cloned and overexpressed in the protease-deficient bacillus subtilis db430 using an escherichia coli-bacillus shuttle vector pbsmul2. the 34,625.13 and 34,675.11-da enzymes were purified from the culture supernatant of b. subtilis expressing the wild-type and mutated genes, respectively. the purified proteases showed the same n-terminal sequences and biochemical properties of thos ... | 2012 | 22178490 |
| production of alkali tolerant cellulase free xylanase in high levels by bacillus pumilus sv-205. | the fermentation conditions were optimized for hyper production of xylanase from bacillus pumilus sv-205. the bacterium secretes high levels (7382.7±1200 iu/ml) of cellulase-free xylanase using wheat bran led to 21.63 fold increase in activity. a combination of yeast extract and peptone stimulated highest xylanase production (2448.0 iu/ml) as compared to other combinations. the most important characteristic of the enzyme is its high ph stability (100%) over a broad ph range of 6-11 for 24h. ther ... | 2012 | 22227307 |
| evidence for two putative holin-like peptides encoding genes of bacillus pumilus strain wapb4. | an open reading frame encoding a 71-amino acid bhla bacteriocin-related holin-like peptide was present upstream of 86-amino acid holin-like peptide, xhlb, encoding gene in the genome of bacillus pumilus strain wapb4. analysis of bhla using tmhmm server suggested one putative transmembrane domain at the n-terminal part and a number of highly charged amino acid residues at the c-terminal part. xhlb of b. pumilus strain wapb4 composed of two putative transmembrane domains separated by a β-turn, and ... | 2012 | 22231453 |
| aerobic degradation of tetrabromobisphenol-a by microbes in river sediment. | this study investigated the aerobic degradation of tetrabromobisphenol-a (tbbpa) and changes in the microbial community in river sediment from southern taiwan. aerobic degradation rate constants (k(1)) and half-lives (t(1/2)) for tbbpa (50μgg(-1)) ranged from 0.053 to 0.077d(-1) and 9.0 to 13.1d, respectively. the degradation of tbbpa (50μgg(-1)) was enhanced by adding yeast extract (5mgl(-1)), sodium chloride (10ppt), cellulose (0.96mgl(-1)), humic acid (0.5gl(-1)), brij 30 (55μm), brij 35 (91μ ... | 2012 | 22245059 |
| identification and characterization of psychrotolerant sporeformers associated with fluid milk production and processing. | psychrotolerant spore-forming bacteria represent a major challenge to the goal of extending the shelf life of pasteurized dairy products. the objective of this study was to identify prominent phylogenetic groups of dairy-associated aerobic sporeformers and to characterize representative isolates for phenotypes relevant to growth in milk. analysis of sequence data for a 632-nucleotide fragment of rpob showed that 1,288 dairy-associated isolates (obtained from raw and pasteurized milk and from dai ... | 2012 | 22247129 |
| small surfactant-like peptides can drive soluble proteins into active aggregates. | inactive protein inclusion bodies occur commonly in escherichia coli (e. coli) cells expressing heterologous proteins. previously several independent groups have found that active protein aggregates or pseudo inclusion bodies can be induced by a fusion partner such as a cellulose binding domain from clostridium cellulovorans (cbdclos) when expressed in e. coli. more recently we further showed that a short amphipathic helical octadecapeptide 18a (ewlkafyekvleklkelf) and a short beta structure pep ... | 2012 | 22251949 |
| essential oil of myrica esculenta buch. ham.: composition, antimicrobial and topical anti-inflammatory activities. | hydrodistilled oil obtained from the stem bark of myrica esculenta buch. ham. ex d. don (yield 0.3%) was analysed by capillary gc and gc-ms. the volatile oil consisted mainly of n-hexadecanol (25.2%), eudesmol acetate (21.9%), palmitic acid (11.6%), cis-β-caryophyllene (8.7%), n-pentadecanol (7.7%) and n-octadecanol (7.6%). the oil was found to be a potential antimicrobial agent against bacillus pumilus, staphylococcus aureus, staphylococcus epidermidis, escherichia coli, pseudomonas aeruginosa, ... | 2012 | 22260222 |
| biodegradation of sewage wastewater using autochthonous bacteria. | the performance of isolated designed consortia comprising bacillus pumilus, brevibacterium sp, and pseudomonas aeruginosa for the treatment of sewage wastewater in terms of reduction in cod (chemical oxygen demand), bod (biochemical oxygen demand) mlss (mixed liquor suspended solids), and tss (total suspended solids) was studied. different parameters were optimized (inoculum size, agitation, and temperature) to achieve effective results in less period of time. the results obtained indicated that ... | 2012 | 22272181 |
| γ-glutamyl transpeptidase from bacillus pumilus ks 12: decoupling autoprocessing from catalysis and molecular characterization of n-terminal region. | gamma glutamyl transpeptidase from bacillus pumilus ks12 (ggtbp) was cloned, expressed in pet-28-e. coli expression system as a heterodimeric enzyme with molecular weights of 45 and 20 kda for large and small subunit, respectively. it was purified by nickel affinity chromatography with hydrolytic and transpeptidase activity of 1.82 u/mg and 4.35 u/mg, respectively. sequence analysis revealed that ggtbp was most closely related to bacillus licheniformis ggt and had all the catalytic residues and ... | 2012 | 22305170 |
| pongamia pinnata seed cake: a promising and inexpensive substrate for production of protease and lipase from bacillus pumilus sg2 on solid-state fermentation. | the production of a protease and a lipase from bacillus pumilus sg2 on solid-state fermentation using pongamia pinnata seed cake as substrate was studied. the seed cake was proved to be a promising substrate for the bacterial growth and the enzyme production. the initial ph, incubation time and moisture content were optimized to achieve maximal enzyme production. maximum protease production was observed at 72 h and that of the lipase at 96 h of incubation. the production of protease (9840 u/g dm ... | 2011 | 22329247 |
| isolation, purification and characterisation of low molecular weight xylanase from bacillus pumilus ssp-34. | low molecular weight endo-xylanase from bacillus pumilus ssp-34 was purified to homogeneity using ion exchange and size exclusion chromatographies. xylanases were isolated by novel purification protocol which includes the use of anion exchange matrix such as deae sepharose cl 6b with less affinity towards enzyme protein. the purified b. pumilus ssp-34 have a molecular weight of 20 kda, with optimum ph and temperature at 6.0 and 50 °c, respectively. the enzyme was stable at 50 °c for 30 min. it s ... | 2012 | 22350873 |
| bilirubin oxidase from bacillus pumilus: a promising enzyme for the elaboration of efficient cathodes in biofuel cells. | a cota multicopper oxidase (mco) from bacillus pumilus, previously identified as a laccase, has been studied and characterized as a new bacterial bilirubin oxidase (bod). the 59 kda protein containing four coppers, was successfully over-expressed in escherichia coli and purified to homogeneity in one step. this 509 amino-acid enzyme, having 67% and 26% sequence identity with cota from bacillus subtilis and bod from myrothecium verrucaria, respectively, shows higher turnover activity towards bili ... | 2012 | 22410485 |
| restoration of eroded soil in the sonoran desert with native leguminous trees using plant growth-promoting microorganisms and limited amounts of compost and water. | restoration of highly eroded desert land was attempted in the southern sonoran desert that had lost its natural capacity for self-revegetation. in six field experiments, the fields were planted with three native leguminous trees: mesquite amargo prosopis articulata, and yellow and blue palo verde parkinsonia microphylla and parkinsonia florida. restoration included inoculation with two of plant growth-promoting bacteria (pgpb; azospirillum brasilense and bacillus pumilus), native arbuscular myco ... | 2012 | 22425876 |
| metabolic flexibility of d-ribose producer strain of bacillus pumilus under environmental perturbations. | the metabolic reaction rate vector is a bridge that links gene and protein expression alterations to the phenotypic endpoint. we present a simple approach for the estimation of flux distribution at key branch points in the metabolic network by using substrate uptake, metabolite secretion rate, and biomass growth rate for transketolase (tkt) deficient bacillus pumilus atcc 21951. we find that the glucose-6-phosphate (g6p) and pseudo catabolic/anabolic branch points are flexible in the d: -ribose- ... | 2012 | 22438109 |
| microbiological and physicochemical characterisation of caxiri, an alcoholic beverage produced by the indigenous juruna people of brazil. | caxiri is a traditional fermented alcoholic beverage produced from cassava and sweet potatoes by the indigenous juruna or yudjá people in brazil. our results showed that caxiri fermentation is invariably associated with the following: (i) an increase in the total microbial population, with yeast being the largest group detected; (ii) a decrease in reducing sugars, malic, tartaric, succinic, oxalic and propionic acid; and (iii) a final product characterised by a high content of ethanol and a high ... | 2012 | 22497838 |
| iodine from bacterial iodide oxidization by roseovarius spp. inhibits the growth of other bacteria. | microbial activities in brine, seawater, or estuarine mud are involved in iodine cycle. to investigate the effects of the microbiologically induced iodine on other bacteria in the environment, a total of 13 bacteria that potentially participated in the iodide-oxidizing process were isolated from water or biofilm at a location containing 131 μg ml(-1) iodide. three distinct strains were further identified as roseovarius spp. based on 16 s rrna gene sequences after being distinguished by restricti ... | 2013 | 22526798 |
| air-dust-borne associations of phototrophic and hydrocarbon-utilizing microorganisms: promising consortia in volatile hydrocarbon bioremediation. | aquatic and terrestrial associations of phototrophic and heterotrophic microorganisms active in hydrocarbon bioremediation have been described earlier. the question arises: do similar consortia also occur in the atmosphere? dust samples at the height of 15 m were collected from kuwait city air, and analyzed microbiologically for phototrophic and heterotrophic hydrocarbon-utilizing microorganisms, which were subsequently characterized according to their 16s rrna gene sequences. the hydrocarbon ut ... | 2012 | 22529000 |
| rapid detection of viable bacillus pumilus safr-032 encapsulated spores using novel propidium monoazide-linked fluorescence in situ hybridization. | the survival of bacillus pumilus safr-032 spores to standard industrial clean room sterilization practices necessitates the development of rapid molecular diagnostic tool(s) for detection and enumeration of viable bacterial spores in industrial clean room environments. this is of importance to maintaining the sterility of clean room processing products. this paper describes the effect of propidium monoazide (pma) on fluorescence in situ hybridization (fish) for detecting and enumerating b. pumil ... | 2012 | 22537819 |
| diversity of spore-forming bacteria and identification of bacillus amyloliquefaciens as a species frequently associated with the ropy spoilage of bread. | this study examines the diversity of spore-forming bacteria isolated from raw materials/bread using molecular methods along with a rapid and innovative technology, the ft-nir spectroscopy. microbiological analysis showed that 23% of semolina and 42% of other raw materials (including grain, brewer yeast, improvers) contained more than 100 spores/g and more than 50% of each kind of sample was contaminated at a level ranging from 1 to 100 spores/g. a high bacterial diversity characterized raw mater ... | 2012 | 22551674 |
| antimicrobial potency of the leaf - stalk extract of curcuma longa (linn). | the methanolic extract of the leaf-stalk of curcuma longa linn, was tested for its minimum inhibitor concentration (mic) against gram positive-staphylococcus aureus, bacillus pumilus, bacillus subtilis, klebsiella pnemoniae, bacillus cereus, streptococcus pneumoniae, lactobacillus arabinosus and gram negative e.coli, shigella dysenteriae, shigella sonnei, shigella boydii, salmonella typhimurium, proteus mirabilis, and vibrio cholerae strains, further, the ones of inhibition produced by the crude ... | 2000 | 22557005 |
| antimicrobial activity of the mycotoxin citrinin obtained from the fungus penicillium citrinum. | the mycotoxin citrinin was obtained from the fungus penicillium citrinum. it was tested for it's minimum inhibitory concentration (mic) against some gram positive strains viz. staphylococcus aureus, bacillus pumilus, bacillus subtillis, bacillus cereus, klebsiella pneumoniae, streptococcus pneumoniae, lactobacillus arabinosus and gram negative strains e.coli, shigella dysenteriae, shigella sonnei, shigella boydii, salmonella typhimurium, proteus mirabilis and vibrio cholerae. further the zones o ... | 2002 | 22557053 |
| comparative microbiological activities of ethanolic extracts of roots and aerial parts of achyranthes aspera linn. | ethanolic extract of roots and aerial parts of achyranthes aspera was investigated for its antibacterial activity against bacillus subtilis, bacillus pumilus, and proteus vulgaris and anti fungal activity against cryptococcus neoformans, aspergillus flavus at 100 mg/disc using diffusion method. the comparative studies shown that ethanolic extract of roots and aerial parts of achyranthes aspera have exhibited moderately equal action when compared to cotrimoxazole (30 mg/disc) for antibacterial an ... | 2003 | 22557101 |
| antibacterial effect of bulbophyllum neilgherrense wt. (orchidaceae). an in vitro study. | the antibacterial activity of bulbophyllum neilgherrense was tested against five species of bacteria, viz., escherichia coli, staphylococcus aureus, bacillus pumilus, pseudomonas aeruginosa and pseudomonas putida aqueous, ethanolic and chloroform soluble extracts of leaf and pseudobulb (5.50 w/v) were screened for their antibacterial potential. the zone of inhibition was seen to be largest when alcoholic extract was used. | 2005 | 22557190 |
| antimicrobial properties of roots of medicinal plants. | antibacterial properties of hexane, chloroform and aqueous extracts of roots of acorus calamus, aristolochia indica, cyperus rotundus, desmodium gangeticum, holostemma ada- kodien and kaempferia galanga, used in the traditional medicine were studied on bacillus pumilis and eschericia coli by disc diffusion method. | 2005 | 22557193 |
| preliminary phytochemical screening and antimicrobial studies on artocarpus lakoocha roxb. | the antibacterial activity of artocarpus lakoocha roxb. bark was tested against different species of bacteria. the minimum inhibitory concentration (mic) of the methanolic extract showed that there was no growth in shigella soneii 2, e.coli mtcc 1568, bacillus pumilus 8241, proteus mirabilis am 198, bacillus subtelis atcc 6633, e. coli row 7/12 species at 200-400g/ml. from the result obtained, it was observed that the methanolic extract of. artocarpus lakoocha at concentration of 200 gl ml and 4 ... | 2009 | 22557328 |
| nonrecovery of varying proportions of viable bacteria during spread plating governed by the extent of spreader usage and proposal for an alternate spotting-spreading approach to maximize the cfu. | to elucidate the cause of high variations and inconsistencies in bacterial cfu observed within and between different experiments while assessing viable bacterial counts through spread plating (sp). | 2012 | 22563785 |
| characterization of boron resistant and accumulating bacteria lysinibacillus fusiformis m1, bacillus cereus m2, bacillus cereus m3, bacillus pumilus m4 isolated from former mining site, hokkaido, japan. | boron is known to be widespread environmental contaminant that is relatively mobile in soil when compared to other metal contaminants. the present study made an attempt to isolate and characterize the boron resistant and accumulating bacteria from former mining site at hokkaido, japan. four potential strains m1, m2, m3 and m4 were selected based on high degree of boron and heavy metal resistances. the morphological, biochemical and 16s rdna sequencing analysis of mining bacteria revealed that th ... | 2012 | 22571522 |
| cold atmospheric air plasma sterilization against spores and other microorganisms of clinical interest. | physical cold atmospheric surface microdischarge (smd) plasma operating in ambient air has promising properties for the sterilization of sensitive medical devices where conventional methods are not applicable. furthermore, smd plasma could revolutionize the field of disinfection at health care facilities. the antimicrobial effects on gram-negative and gram-positive bacteria of clinical relevance, as well as the fungus candida albicans, were tested. thirty seconds of plasma treatment led to a 4 t ... | 2012 | 22582068 |
| resistance of bacterial endospores to outer space for planetary protection purposes--experiment protect of the expose-e mission. | spore-forming bacteria are of particular concern in the context of planetary protection because their tough endospores may withstand certain sterilization procedures as well as the harsh environments of outer space or planetary surfaces. to test their hardiness on a hypothetical mission to mars, spores of bacillus subtilis 168 and bacillus pumilus safr-032 were exposed for 1.5 years to selected parameters of space in the experiment protect during the expose-e mission on board the international s ... | 2012 | 22680691 |
| survival of bacillus pumilus spores for a prolonged period of time in real space conditions. | to prevent forward contamination and maintain the scientific integrity of future life-detection missions, it is important to characterize and attempt to eliminate terrestrial microorganisms associated with exploratory spacecraft and landing vehicles. among the organisms isolated from spacecraft-associated surfaces, spores of bacillus pumilus safr-032 exhibited unusually high resistance to decontamination techniques such as uv radiation and peroxide treatment. subsequently, b. pumilus safr-032 wa ... | 2012 | 22680694 |
| viability of phenanthrene biodegradation by an isolated bacterial consortium: optimization and scale-up. | in the present work, biodegradation of phenanthrene by a bacterial consortium (lb2), isolated from lab-polluted soils has been investigated. the 16s rrna gene-based molecular analysis revealed that the bacterial consortium lb2 consisted of two strains showing a very high homology with staphylococcus warneri and bacillus pumilus. the optimization of phenanthrene degradation by the consortium lb2, using a central composite face-centered design was carried out taking into account three important pa ... | 2013 | 22717666 |
| efficient pahs biodegradation by a bacterial consortium at flask and bioreactor scale. | in this work, the biodegradation of three polycyclic aromatic hydrocarbons (pahs) such as phenanthrene (phe), pyrene (pyr) and benzo[a]anthracene (baa) has been investigated. a bacterial consortium consisting of two strains was used for the first time based on preliminary promising biodegradation data. they were tentatively identified as staphylococcus warneri and bacillus pumilus. degradation values higher than 85% were obtained for each single pah when operating at flask scale, whereas minimum ... | 2012 | 22738812 |
| protection of bacillus pumilus spores by catalases. | bacillus pumilus safr-032, isolated at spacecraft assembly facilities of the national aeronautics and space administration jet propulsion laboratory, is difficult to kill by the sterilization method of choice, which uses liquid or vapor hydrogen peroxide. we identified two manganese catalases, yjqc and bpum_1305, in spore protein extracts of several b. pumilus strains by using page and mass spectrometric analyses. while the bpum_1305 catalase was present in six of the b. pumilus strains tested, ... | 2012 | 22752169 |
| capability of a selected bacterial consortium for degrading diesel/biodiesel blends (b20): enzyme and biosurfactant production. | the search for alternative sources of energy, such as biodiesel, has been stimulated, since this biofuel is highly susceptible for biodegradation and has low toxicity, thus, reducing the impact in ecosystems. the objective of this study was to select a bacterial consortium with potential for degrading diesel/biodiesel blends (b20) obtained from areas contaminated with hydrocarbons/esters. in order to evaluate the biodegrability of the blend, six enzyme assays were conducted: alkane hydroxylase, ... | 2012 | 22755524 |
| swapping of pro-sequences between keratinases of bacillus licheniformis and bacillus pumilus: altered substrate specificity and thermostability. | pro-sequences were swapped in cis between keratinases from bacillus licheniformis (ker bl) and bacillus pumilus (ker bp) to construct ker probp-bl and ker probl-bp, respectively. expression of these keratinases was carried out constitutively by e. coli hb101-pezz18 system. they were characterized with respect to their parent enzymes, ker bl and ker bp, respectively. ker probp-bl became more thermostable with a t(1/2) of 45 min at 80°c contrary to ker bl which was not stable beyond 60°c. similarl ... | 2012 | 22759531 |
| heat and drying time modulate the o2 reduction current of modified glassy carbon electrodes with bilirubin oxidases. | here we show that the magnitude of the o(2) reduction current of cathodes based on bilirubin oxidases (bod) immobilized into a redox hydrogel strongly depends on the drying conditions such as the curing time and temperature of drying as well as the thermostability of the bod. to illustrate this effect, we performed experiments with two different bods: one labile bod from trachyderma tsunodae and one highly thermostable bod from bacillus pumilus with different preparation protocols. the balance b ... | 2012 | 22772078 |
| [subtilisin like protease secreted in bacillus pumilus kmm 62 on different growth stages]. | a protease secreted in bacillus pumilus kmm 62 culture liquid on different growth stages was isolated using ion-exchange chromatography. on the basis of pattern of specific chromogenic substrates hydrolysis and inhibitory analysis the protease was classified as subtilisin like serine protease. the molecular weight ofprotease is 31 kda. proteolytic activity towards z-ala-ala-leu-pna substrate was maximal at ph 8-8.5. the optimal temperature for proteolytic activity was observed at a temperature o ... | 2012 | 22792728 |
| scale-up of an alkaline protease from bacillus pumilus mtcc 7514 utilizing fish meal as a sole source of nutrients. | fish meal grades sl1 and sl2 from sardine (sardinella longiceps) and nj from pink perch (nemipterus japonicas) were evaluated as a sole source of carbon and nitrogen in the medium for alkaline protease production by bacillus pumilus mtcc 7514. the analysis of the fish meal suggests that the carbon and nitrogen contents in fish meal are sufficient to justify its choice as replacement for other nutrients. protease production increased significantly (4,914 u/ml) in medium containing only fish meal, ... | 2012 | 22814497 |
| improving genetic immobilization of a cellulase on yeast cell surface for bioethanol production using cellulose. | in this study, saccharomyces cerevisiae was genetically engineered to harbor the capability of utilizing celluloses for bioethanol production by displaying active cellulolytic enzymes on the cell surface. an endo-1,4-β-glucanase gene egx was cloned from bacillus pumilus c-9 and its expression products, the egx cellulases, were displayed on the cell surface of s. cerevisiae by fusing egx with aga2 that encodes the binding subunit of the s. cerevisiae cell wall protein α-agglutinin. to achieve hig ... | 2013 | 22915066 |
| biotechnological potential of agro residues for economical production of thermoalkali-stable pectinase by bacillus pumilus dcsr1 by solid-state fermentation and its efficacy in the treatment of ramie fibres. | the production of a thermostable and highly alkaline pectinase by bacillus pumilus dcsr1 was optimized in solid-state fermentation (ssf) and the impact of various treatments (chemical, enzymatic, and in combination) on the quality of ramie fibres was investigated. maximum enzyme titer (348.0 ± 11.8 ug(-1) dbb) in ssf was attained, when a mixture of agro-residues (sesame oilseed cake, wheat bran, and citrus pectin, 1 : 1 : 0.01) was moistened with mineral salt solution (a(w) 0.92, ph 9.0) at a su ... | 2012 | 22928091 |
| enhanced remediation of chlorpyrifos from soil using ryegrass (lollium multiflorum) and chlorpyrifos-degrading bacterium bacillus pumilus c2a1. | the combined use of plants and associated microorganisms has great potential for remediating soil contaminated with organic compounds such as pesticides. the objective of this study was to determine whether the bacterial inoculation influences plant growth promotion and chlorpyrifos (cp) degradation and accumulation in different parts of the plant. ryegrass was grown in soil spiked with cp and inoculated with a pesticide degrading bacterial strain bacillus pumilus c2a1. inoculation generally had ... | 2012 | 22959266 |
| chitin extraction from shrimp shell waste using bacillus bacteria. | the ability of six protease-producing bacillus species (bacillus pumilus a1, bacillus mojavencis a21, bacillus licheniformis rp1, bacillus cereus sv1, bacillus amyloliquefaciens an6 and bacillus subtilis a26) to ferment media containing only shrimp shell waste, for chitin extraction, was investigated. more than 80% deproteinization was attained by all the strains tested. however, demineralization rates not exceeding 67% were registered. cultures conducted in media containing shrimp shell waste s ... | 2012 | 22981824 |
| antitrypanosomal alkaloids from the marine bacterium bacillus pumilus. | fractionation of the ethyl acetate extract of the marine bacterium bacillus pumilus isolated from the black coral antipathes sp. led to the isolation of five compounds: cyclo-(l-leu-l-pro) (1), 3-hydroxyacetylindole (2), n-acetyl-β-oxotryptamine (3), cyclo-(l-phe-l-pro) (4), and 3-formylindole (5). the structures of compounds 1-5 were established by spectroscopic analyses, including hresitof-ms and nmr (1h, 13c, hsqc, hmbc and cosy). compounds 2, 3 and 5 caused the inhibition on the growth of tr ... | 2012 | 22990456 |
| effect of plant growth-promoting rhizobacteria (pgprs) on plant growth, yield, and quality of tomato (lycopersicon esculentum mill.) under simulated seawater irrigation. | to determine the effects of three pgprs on plant growth, yield, and quality of tomato under simulated seawater irrigation, a two consecutive seasons' field experiment was conducted in yancheng teachers university plot from april to june and august to october, 2011. the results showed that erwinia persicinus ra2 containing acc deaminase exhibited the best ability compared with bacillus pumilus wp8 and pseudomonas putida rbp1 which had no acc deaminase activity to enhance marketable yields of fres ... | 2012 | 22990485 |
| identification of the potential of microbial combinations obtained from spent mushroom cultivation substrates for use in textile effluent decolorization. | the study presents variation in microbial population of agaricus bisporus, pleurotus sajor-caju and volvariella volvacea spent substrates (sms) along with ligninolytic enzymes activity and textile effluent decolorization potential of microorganisms isolated from these. the effect of temperature, ph, carbon sources and immobilizing agents on effluent decolorization using different combinations of these microorganisms has also been studied. sms of p. sajor-caju harbored highest population and dive ... | 2012 | 23026337 |
| highly active β-xylosidases of glycoside hydrolase family 43 operating on natural and artificial substrates. | the hemicellulose xylan constitutes a major portion of plant biomass, a renewable feedstock available for conversion to biofuels and other bioproducts. β-xylosidase operates in the deconstruction of the polysaccharide to fermentable sugars. glycoside hydrolase family 43 is recognized as a source of highly active β-xylosidases, some of which could have practical applications. the biochemical details of four gh43 β-xylosidases (those from alkaliphilus metalliredigens qymf, bacillus pumilus, bacill ... | 2013 | 23053115 |
| high-antibacterial activity of urtica spp. seed extracts on food and plant pathogenic bacteria. | the aim of this study was to comparatively evaluate antibacterial activities of methanol (metoh) and aqueous (dw) leaf (l), root (r) and seed (s) extracts of urtica dioica l. (ud; stinging nettle) and urtica pilulifera l. (up; roman nettle) on both food- and plant-borne pathogens, with total phenolic contents and dpph radical scavenging activities (drsa). metoh extracts of leaves and roots of u. dioica had the highest drsa. extracts with high antibacterial activity were in the order up-lmetoh (1 ... | 2013 | 23067263 |
| characterization of thermostable serine alkaline protease from an alkaliphilic strain bacillus pumilus mcas8 and its applications. | this study describes the characterization and optimization of medium components for an extracellular detergent, surfactant, organic solvent and thermostable serine alkaline protease produced by alkaliphilic bacillus pumilus mcas8 strain isolated from pulicat lake sediments, tamil nadu, india. the strain yielded maximum protease (2,214 u/ml) under optimized conditions: carbon source, citric acid-1.5 % (w/w); inducer, soyabean meal-2 % (w/w); ph 11.0; shaking condition 37 °c for 48 h. the enzyme h ... | 2012 | 23076563 |
| exiguobacterium oxidotolerans, a halotolerant plant growth promoting rhizobacteria, improves yield and content of secondary metabolites in bacopa monnieri (l.) pennell under primary and secondary salt stress. | brahmi (bacopa monnieri), an integral component of indian ayurvedic medicine system, is facing a threat of extinction owing to the depletion of its natural populations. the present study investigates the prospective of exploitation of halotolerant plant growth promoting rhizobacteria (pgpr) in utilising the salt stressed soils for cultivation of b. monnieri. the effects of two salt tolerant pgpr, bacillus pumilus (str2) and exiguobacterium oxidotolerans (str36) on the growth and content of bacos ... | 2013 | 23085953 |
| [influence of bacteria of bacillus genus on the causative agent of bacterial cancer of tomatoes]. | it has been shown that bacteria of the genus bacillus inhibited the development of infection caused by clavibacter michiganensis subsp. michiganensis, in tomatoes. pre-sowing seed treatment with suspensions of bacillus subtilis imv b-7023 and bacillus pumilus 3 enhanced resistance of plants to bacterial disease of cancer, probably due to the synthesis of biologically active substances with antimicrobial properties by these bacteria. of the two strains of bacillus, differing by antagonist propert ... | 2013 | 23120989 |
| activation of bacillus spores at moderately elevated temperatures (30-33 °c). | the time/temperature profiles experienced by spores on the track from their natural sporulation environment to consumable food products may be highly diverse. temperature has been documented as an important factor that may activate spores, i.e. potentiates spores to germinate. there is, however, limited knowledge about the relationship between the expected temperature history and the subsequent germination characteristics of bacterial spores. we show here that the germination rate of five differ ... | 2013 | 23132276 |
| draft genome sequence of bacillus pumilus ba06, a producer of alkaline serine protease with leather-dehairing function. | bacillus pumilus ba06 was isolated from the proteinaceous soil and produced an extracellular alkaline protease with leather-dehairing function. the genome of ba06 was sequenced. the comparative genome analysis indicated that strain ba06 is different in genome from the other b. pumilus strains, with limited insertions, deletions, and rearrangements. | 2012 | 23144411 |
| efficacy of using harmless bacillus endospores to estimate the inactivation of cryptosporidium parvum oocysts in water. | the need to use complex in vitro cell culture, expensive equipment, and highly-trained technicians that are available only to specialist laboratories has significantly limited studies assessing the potential of pulsed uv light (puv) to inactivate the waterborne parasite cryptosporidium parvum in drinking water. this constitutes the first study to report on the use of different non-pathogenic bacillus endospores as potential surrogate organisms to indicate the puv inactivation performance of a c. ... | 2013 | 23145570 |
| [cell wall teichoic acids of bacillus licheniformis vkm b-511t, bacillus pumilus vkm b-508t, and other strains previously assigned to bacillus pumilus]. | 2012 | 23156690 | |
| [the novel adams-like microbial metalloendopeptidase]. | heterologous gene expression of extracellular minor metalloendopeptidase of bacillus pumilus 3-19 in protease-deficient b. subtilis strain has been studied. the fraction of enzyme in total pool of b. pumilus 3-19 secreted proteases composes less than 8%. the enzyme was isolated from culture liquid of recombinant strain, its primary structure was determined, physicochemical properties were investigated. it was concluded that secreted metallo endopeptidase of b. pumilus 3-19 represents the first p ... | 2012 | 23189558 |