Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| expression in escherichia coli of native and chimeric phenolic acid decarboxylases with modified enzymatic activities and method for screening recombinant e. coli strains expressing these enzymes. | four bacterial phenolic acid decarboxylases (pad) from lactobacillus plantarum, pediococcus pentosaceus, bacillus subtilis, and bacillus pumilus were expressed in escherichia coli, and their activities on p-coumaric, ferulic, and caffeic acids were compared. although these four enzymes displayed 61% amino acid sequence identity, they exhibit different activities for ferulic and caffeic acid metabolism. to elucidate the domain(s) that determines these differences, chimeric pad proteins were const ... | 2001 | 11229892 |
| characterization and growth of bacillus spp. in heat-treated cream with and without nisin. | to study the germination and growth of both inoculated and naturally occurring bacillus strains in heat-treated cream with and without nisin. | 2001 | 11309063 |
| over-expression and properties of a purified recombinant bacillus licheniformis lipase: a comparative report on bacillus lipases. | the gene coding for an extracellular lipase of bacillus licheniformis was cloned using pcr techniques. the sequence corresponding to the mature lipase was subcloned into the pet 20b(+) expression vector to construct a recombinant lipase protein containing 6 histidine residues at the c-terminal. high-level expression of the lipase by escherichia coli cells harbouring the lipase gene-containing expression vector was observed upon induction with iptg at 30 degrees c. a one step purification of the ... | 2001 | 11339956 |
| analysis of a conserved hydrophobic pocket important for the thermostability of bacillus pumilus chloramphenicol acetyltransferase (cat-86). | site-directed mutagenesis was carried out on bacillus pumilus chloramphenicol acetyltransferase (cat-86) to determine the effects of substitution at a conserved hydrophobic pocket identified earlier as important for thermostability. mutations were introduced that would substitute residues at consensus positions 33, 191 and 203 in the enzyme, both individually and in combination. two mutants, sdm1 (cat-86 y33f, a203v) and sdm5 (cat-86 a203i), were more thermostable than wild-type and two mutants, ... | 2001 | 11342712 |
| toxic-metabolite-producing bacteria and fungus in an indoor environment. | toxic-metabolite-emitting microbes were isolated from the indoor environment of a building where the occupant was suffering serious building-related ill-health symptoms. toxic substances soluble in methanol and inhibitory to spermatozoa at <10 microg (dry weight) ml(-1) were found from six bacterial isolates and one fungus. the substances from isolates of bacillus simplex and from isolates belonging to the actinobacterial genera streptomyces and nocardiopsis were mitochondriotoxic. these substan ... | 2001 | 11425751 |
| toxic bacillus pumilus from indoor air, recycled paper pulp, norway spruce, food poisoning outbreaks and clinical samples. | forty-four b. pumilus isolates of food poisoning, clinical, environmental and industrial origins were investigated for toxin production using the boar spermatozoan motility assay, previously shown to be a sensitive method for detecting non-protein toxins from b. cereus and b. licheniformis. the three toxic isolates originated from live tree, indoor air and recycled paper pulp and were more toxic than the previously described food poisoning isolates of b. licheniformis, whereas the b. pumilus foo ... | 2001 | 11518331 |
| characterization of plant growth promoting rhizobacteria isolated from polluted soils and containing 1-aminocyclopropane-1-carboxylate deaminase. | fifteen bacterial strains containing 1-aminocyclopropane-1-carboxylate (acc) deaminase were isolated from the rhizoplane of pea (pisum sativum l.) and indian mustard (brassica juncea l.) grown in different soils and a long-standing sewage sludge contaminated with heavy metals. the isolated strains were characterized and assigned to various genera and species, such as pseudomonas brassicacearum, pseudomonas marginalis, pseudomonas oryzihabitans, pseudomonas putida, pseudomonas sp., alcaligenes xy ... | 2001 | 11547884 |
| isolation and characterization of a cell-associated protein of bacillus pumilus ph-01. | a cell-associated protein released from bacillus pumilus ph-01 showed an affinity for some dioxins, like 1,2,3,4-tetrachlorodibenzo-p-dioxin (tcdd) and 1,2,3,4-tetrachlorodibenzofuran (tcdf), and the concentration of the protein increased when b. pumilus ph-01 was boiled in minimal salts medium. sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-mass spectrometry revealed that the boiled culture supernatant contained a major protein with a m ... | 2001 | 11549009 |
| heterologous expression of the bacillus pumilus endo-beta-xylanase (xyna) gene in the yeast saccharomyces cerevisiae. | the endo-beta-xylanase-encoding gene (xyna) of bacillus pumilus pls was isolated from a genomic dna library and the open reading frame (orf) was inserted in expression vectors for the yeast saccharomyces cerevisiae. plasmid pfn3 harboured the xyna orf fused to the yeast mating pheromone alpha-factor signal sequence (mfalpha1s) under the control of the alcohol dehydrogenase ii gene promotor (adh2p) and terminator (adh2t) sequences. in plasmid pfn4, the mfalpha1s-xyna orf was brought under the con ... | 2001 | 11549015 |
| cloning, molecular characterization, and application of rice epiphytic bacillus pumilus promoter fragments. | to establish a constitutive, high-efficiency expression system for bacillus pumilus (b.p), we cloned random chromosomal dna into promoter probe shuttle vector ece7 and selected for strong promoter activity by chloramphenicol resistance of transformed b. pumilus cells. the nucleotide sequences of nine chromosomal fragments were determined. these dna fragments range from 300 to 2200 bp in size. the transcription strength of these promoters was estimated by determination of cat enzyme production in ... | 2001 | 11683357 |
| fermentation of starch for enhanced alkaline protease production by constructing an alkalophilic bacillus pumilus strain. | a new engineering strain, bacillus pumilus c172-14 (pbx 96), was obtained by introducing the pbx 96 plasmid, which carries the alpha-amylase amy gene, into the host strain of alkalophilic bacillus pumilus c172 via transformation. the newly constructed strain was found to express the amy gene and could use starch instead of glucose or starch hydrolysate as carbon source for its fermentation of alkaline protease. the pbx 96 plasmid in the new host was found to be segregationally and structurally s ... | 2001 | 11693913 |
| purification, crystallization and preliminary x-ray analysis of an acetylxylan esterase from bacillus pumilus. | the gene encoding for acetylxylan esterase from bacillus pumilus has been cloned and expressed in escherichia coli. the recombinant protein has been purified to homogeneity and crystallized. the crystals obtained are of regular shape of dimensions 0.05 x 0.05 x 0.05 mm with r32 symmetry and diffract to 2.0 a using synchrotron radiation. | 2001 | 11717513 |
| consumption of tropospheric levels of methyl bromide by c(1) compound-utilizing bacteria and comparison to saturation kinetics. | pure cultures of methylotrophs and methanotrophs are known to oxidize methyl bromide (mebr); however, their ability to oxidize tropospheric concentrations (parts per trillion by volume [pptv]) has not been tested. methylotrophs and methanotrophs were able to consume mebr provided at levels that mimicked the tropospheric mixing ratio of mebr (12 pptv) at equilibrium with surface waters ( approximately 2 pm). kinetic investigations using picomolar concentrations of mebr in a continuously stirred t ... | 2001 | 11722890 |
| typing and subtyping of 83 clinical isolates purified from surgically implanted silicone feeding tubes by random amplified polymorphic dna amplification. | in this study, 83 clinical isolates purified from biofilms colonizing 18 silicone gastrostomy devices (12 "buttons" and six tubes converted to skin level devices) were selected for subtype characterization utilizing genetic analysis. the tubes, previously used for feeding, remained in place for 3 to 47 months (mean, 20.0 months) in children ranging in age from 6 months to 17 years. classification of specific microbes using random amplified polymorphic dna (rapd) analysis revealed genetic similar ... | 2002 | 11825951 |
| preparative chromatography of xylanase using expanded bed adsorption. | expanded bed adsorption was used to purify a marketable xylanase often used in the kraft pulp bleaching process. experiments in packed and expanded beds were carried out mainly to study the adsorption of xylanase on to a cationic adsorbent (streamline sp) in the presence of cells. in order to study the presence of cells, a bacillus pumilus mass (5% wet mass) was mixed with the enzyme extract and submitted to an expanded bed adsorption system. one xylanase was purified to homogeneity in the packe ... | 2002 | 11831756 |
| bacillus endophyticus sp. nov., isolated from the inner tissues of cotton plants (gossypium sp.). | four strains of aerobic, endospore-forming bacteria were isolated from the inner tissues of healthy cotton plants (gossypium sp., dushanbe, tajikistan). the organisms had identical randomly amplified polymorphic dna patterns that distinguished them from other bacilli that are commonly isolated from plant tissues, e.g. bacillus amyloliquefaciens, bacillus licheniformis, bacillus pumilus and bacillus subtilis. pcr amplification of 16s-23s rrna spacer regions suggested that the four strains could b ... | 2002 | 11837291 |
| on calculating sterility in thermal preservation methods: application of the weibull frequency distribution model. | a simple and parsimonious model which originated from the weibull frequency distribution was proposed to describe nonlinear survival curves of spores. this model was suitable for downward concavity curves (bacillus cereus and bacillus pumilus), as well as for upward concavity curves (clostridium botulinum). it was shown that traditional f values calculated from this new model were no longer additive, to such an extent that a heat treatment should be better characterized by the obtained decimal r ... | 2002 | 11843401 |
| purification and characterization of a thermostable alkaline protease from alkalophilic bacillus pumilus. | an investigation was carried out on the purification and characterization of an alkaline protease from bacillus pumilus mk6-5. | 2002 | 11849485 |
| purification and characterization of 3,3-dihydroxyazetidine from culture medium of bacillus mesentericus and b. subtilis. | a method is described for the purification of 3,3-dihydroxyazetidine (dha), which accelerates the growth of bifidobacterium spp., from the culture medium of bacillus mesentericus (bm). purification involved adsorption to an ion-exchange resin; it required less time and gave a higher yield than a previously reported method. monitoring the inhibition of e. coli nihj jc-2, we searched for other strains that produced 3,3-dihydroxyazetidine. we found that not only b. mesentericus to-a but also b. sub ... | 2002 | 11943362 |
| purification of bacillus pumilus beta-d-xylosidase by affinity chromatography. | 1970 | 11945520 | |
| effects of inoculation with pgpr bacillus and pisolithus tinctorius on pinus pinea l. growth, bacterial rhizosphere colonization, and mycorrhizal infection. | the effect of co-inoculation with pisolithus tinctorius and a pgpr belonging to the genus bacillus (bacillus licheniformis cect 5106 and bacillus pumilus cect 5105) in enhancing growth of pinus pinea plants and the changes that occurred in rhizosphere microbial communities and the degree of mycorrhization were evaluated. both bacterial strains of bacillus promote the growth of pinus pinea seedlings, but this biological effect does not imply a synergic effect with mycorrhizal infection. however, ... | 2001 | 12032619 |
| expression and characterization of ca(2+)-independent lipase from bacillus pumilus b26. | a lipase-producing bacillus pumilus strain (b26) was isolated from a soil sample collected in korea. the cloned gene showed that the lipase b26 composed of a 34-amino-acid signal sequence and a 181-amino-acid mature part corresponding to a molecular mass (m(r)) of 19,225. based on the m(r) and the protein sequence, the lipase b26 belongs to the lipase family i.4. the optimum temperature and ph of the purified enzyme were 35 degrees c and 8.5, respectively. the lipase b26 showed a 'ca(2+)-indepen ... | 2002 | 12117564 |
| the radiation resistance of ascospores and sclerotia of pyronema domesticum. | the food and drug administration has become aware of several instances where supposedly sterile medical surgical products made of chinese cotton have been found to contain viable pyronema domesticum. the aim of this research was to determine the gamma and electron beam radiation resistance values for the two dormant phases (ascospores and sclerotia) of p. domesticum. the resistance values were obtained by developing a standardized system to cultivate, purify, and harvest biological indicators co ... | 2002 | 12161770 |
| reduction of cr(vi) and bioaccumulation of chromium by gram positive and gram negative microorganisms not previously exposed to cr-stress. | resistance to cr(vi) is usually associated with its cellular exclusion, precluding enrichment techniques for the isolation of organisms accumulating cr(vi) via bioreduction to insoluble cr(iii). a technique was developed to screen for potential cr(vi) reduction in approx. 2000 isolates from a coastal environment, based on the non-specific reduction of selenite and tellurite to se0 and te0, and reduction of tetrazolium blue to insoluble blue formazan. the most promising strains were further scree ... | 2002 | 12164635 |
| bacillus species are present in chewing tobacco sold in the united states and evoke plasma exudation from the oral mucosa. | five bacillus species, predominantly bacillus megaterium and bacillus pumilus, were isolated from two popular brands of commercially available chewing tobacco [(5.0 +/- 1) x 10(6) cfu/ml of supernatant; results for four experiments]. moreover, the supernatant of the bacillus culture evoked plasma exudation from postcapillary venules in the intact hamster cheek pouch, exudation that was mediated by the kallikrein/kinin metabolic pathway. taken together, these data indicate that bacillus species c ... | 2002 | 12204959 |
| induction of defense-related ultrastructural modifications in pea root tissues inoculated with endophytic bacteria. | the stimulation exerted by the endophytic bacterium bacillus pumilus strain se34 in plant defense reactions was investigated at the ultrastructural level using an in vitro system in which root-inducing t-dna pea (pisum sativum l.) roots were infected with the pea root-rotting fungus fusarium oxysporum f. sp. pisi. in nonbacterized roots, the pathogen multiplied abundantly through much of the tissue including the vascular stele, whereas in prebacterized roots, pathogen growth was restricted to th ... | 1996 | 12226427 |
| diversity of endophytic bacterial populations and their interaction with xylella fastidiosa in citrus plants. | citrus variegated chlorosis (cvc) is caused by xylella fastidiosa, a phytopathogenic bacterium that can infect all citrus sinensis cultivars. the endophytic bacterial communities of healthy, resistant, and cvc-affected citrus plants were studied by using cultivation as well as cultivation-independent techniques. the endophytic communities were assessed in surface-disinfected citrus branches by plating and denaturing gradient gel electrophoresis (dgge). dominant isolates were characterized by fat ... | 2002 | 12324338 |
| cloning of high activity xylanase gene from bacillus pumilus pj19. | the xylanase gene from bacillus pumilus pj19 amplified by polymerase chain reaction (pcr) was cloned into pcrii vector and transformed into escherichia coli strain invalphaf'. starting from an atg as an initiator codon, an open reading frame coding for 202 amino acids was obtained. the recombinant xylanase sequence showed a 96% homology with the xylanase sequence from b. pumilus ipo strain and had an estimated molecular weight of 22,474. xylanase activity expressed by e. coli invalphaf' harborin ... | 2002 | 12385974 |
| [development of the biological preparation enatin with broad-range antimicrobial action]. | physiological and biochemical traits of epiphytic spore forming bacteria bacillus pumilis bim v-263 were examined. the nutrient medium and conditions for submerged cultivation of the strain were selected. the growth dynamics and antagonistic activity during cultivation in a laboratory fermenter ankum-2m were studied. the results provide grounds for development of the biological preparation enatin with broad-range antimicrobial effect. the plant-protective and growth-stimulating effect of enatin ... | 2002 | 12449798 |
| identification of non-pseudomonad bacteria from fruit bodies of wild agaricales fungi that detoxify tolaasin produced by pseudomonas tolaasii. | bacterial isolates from wild agaricales fungi detoxified tolaasin, the inducer of brown blotch disease of cultivated mushrooms produced by pseudomonas tolaasii. mycetocola tolaasinivorans and mycetocola lacteus were associated with fruit bodies of wild pleurotus ostreatus and wild lepista nuda, respectively. tolaasin-detoxifying bacteria belonging to other genera were found in various wild mushrooms. an acinetobacter sp. was isolated from fruit bodies of tricholoma matsutake, bacillus pumilus wa ... | 2002 | 12450133 |
| sterilization of plastic containers using electron beam irradiation directed through the opening. | studies were performed to demonstrate the efficacy of a novel electron beam irradiation system for the on-line sterilization of polymeric containers using energetic electrons directed through the container opening. | 2002 | 12452949 |
| purification and some properties of a novel chitosanase from bacillus subtilis kh1. | one of at least two chitosanases secreted in the culture filtrate of bacillus subtilis kh1 was purified by two sequential deae sepharose cl-6b chromatographies, followed by sephacryl s-100 hr gel chromatography. the purified enzyme was homogenous as judged by sds-page. it showed an estimated molecular weight and pi of 28,000 and 8.3, respectively. the enzyme drastically reduced the viscosity of highly deacetylated chitosan substrates, with the subsequent formation of chitooligosaccharides [(glcn ... | 2000 | 12483600 |
| production by bacillus pumilus (msh) of an antifungal compound that is active against mucoraceae and aspergillus species: preliminary report. | a compound produced by bacillus pumilus (msh) that inhibits mucoraceae and aspergillus species is described. fungicidal activity was demonstrated by lawn-spotting and by diffusion through 0.45 microm millipore membranes placed on 5 % sheep-blood agar, nutrient agar, trypticase soy agar and mueller-hinton agar, followed by spore inoculation of the bacterium-free underlying agar surface. with either technique, zones of fungal inhibition correlated with the zone of haemolysis produced by b. pumilus ... | 2003 | 12488568 |
| a thermostable alpha-arabinofuranosidase from xylanolytic bacillus pumilus: purification and characterisation. | bacillus pumilus ps213 secretes an alpha-l-arabinofuranosidase (af) when grown in the presence of arabinogalactan or oat meal. the enzyme has been purified to homogeneity and characterised. its molecular mass, as determined by gel filtration, is 220 kda, while sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) showed a single band of approximately 60 kda. according to the result of the mass spectrometry analysis showing a molecular mass of 56 kda, the enzyme should be a homote ... | 2003 | 12523971 |
| initial characterization of new bacteria degrading high-molecular weight polycyclic aromatic hydrocarbons isolated from a 2-year enrichment in a two-liquid-phase culture system. | to characterize some polycyclic aromatic hydrocarbons (pah)-degrading microorganisms isolated from an enriched consortium degrading high molecular weight (hmw) pahs in a two-liquid-phase (tlp) soil slurry bioreactor, and to determine the effect of low molecular weight (lmw) pah on their growth and hmw pah-degrading activity. | 2003 | 12534823 |
| [cloning and dna sequencing of bacillus pumilus endo-1,4-beta-glucanase gene]. | bacillus pumilus s-27 screened from marine environment produces an extracellular endo-1,4-beta-glucanase. the enzyme has its optimal activity at ph 5.0-7.0 and 55 degrees c and still retained 79.0% of it at ph 9.0. the gene encoded this endo-1,4-beta-glucanase was cloned and sequenced. the structural gene contained an open reading frame of 1980 bp, corresponding to 660 amino acids, the amino acid sequence of this enzyme is very close to that of an eg of c. cellulovorans and an eg of bacillus sp. ... | 2001 | 12549193 |
| purification and characterization of an extracellular alkaline serine protease with dehairing function from bacillus pumilus. | an extracellular alkaline serine protease (called dhap), produced by a bacillus pumilus strain, demonstrates significant dehairing function. this protease is purified by hydrophobic interaction chromatography, ion exchange, and gel filtration. dhap had a pi of 9.0 and a molecular weight of approximately 32,000 dalton. it shows maximal activity at ph 10 and with a temperature of 55 degrees c; the enzyme activity can be completely inhibited by phenylmethylsulfonyl fluoride (pmsf) and diisopropyl f ... | 2003 | 12567238 |
| xylan-hydrolyzing enzyme system from bacillus pumilus cbmai 0008 and its effects on eucalyptus grandis kraft pulp for pulp bleaching improvement. | the extracellular productions of beta-xylanase, beta-xylosidase, beta-glucosidase, beta-mannanase, arabinosidase, alpha-glucuronidase, alpha-galactosidase and fpase from bacillus pumilus cbmai 0008 were investigated with three different xylan sources as substrate. the enzymatic profiles on birchwood, eucalyptus grandis and oat were studied at alkaline and acidic ph conditions. b. pumilus cbmai 0008 grown on the three carbon sources produced mainly beta-xylanase. at ph 10, the levels of xylanase ... | 2003 | 12573558 |
| effects of sterilizing gamma irradiation on bloodspot newborn screening tests and whole blood cyclosporine and tacrolimus measurements. | sterilizing irradiation of the us mail has been proposed as a method to prevent delivery of viable anthrax spores. because newborn screening samples (bloodspots) and cyclosporine and tacrolimus specimens (whole blood) are delivered routinely through the mail, we studied whether sterilizing gamma irradiation could affect these test results. specimens were exposed to 18 kgy gamma irradiation (100 hours x 18,000 rad/h), a "kill dose" for bacillus pumilus spore strips. irradiation had no significant ... | 2003 | 12580001 |
| degradation of proteins during the fermentation of african locust bean (parkia biglobosa) by strains of bacillus subtilis and bacillus pumilus for production of soumbala. | to examine isolates of bacillus subtilis and b. pumilus predominant in soumbala for their ability to degrade african locust bean proteins (albp). | 2003 | 12588548 |
| novel growth media for rearing larval horn flies, haematobia irritans (diptera: muscidae). | experiments were conducted to develop an agar-based medium for rearing immature horn flies, hematobia irritans (l.). larval survival was determined on sterilized manure inoculated with pure and mixed cultures of acinetobacter sp., bacillus pumilus meyer & gottheil, comamonas acidovorans den dooren de jong, pseudomonas mendocina palleroni, flavobacterium sp. and empedobacter breve (holmes & owen). rearing larvae on mixed cultures enhanced pupal weight but not survival. horn fly larvae failed to s ... | 2003 | 12597649 |
| carbohydrate esterase family 4 enzymes: substrate specificity. | the substrate specificity of selected enzymes classified under carbohydrate esterase family 4 (ce4) has been examined. chitin deacetylase from mucor rouxii and both a native and a truncated form of acetyl xylan esterase from streptomyces lividans were found to be active on both xylan and several soluble chitinous substrates. furthermore, the activities of all enzymes examined were significantly increased in the presence of co(2+) when chitinous substrates were employed. however, the presence of ... | 2003 | 12644381 |
| probiotic mixture decreases dna adduct formation in colonic epithelium induced by the food mutagen 2-amino-9h-pyrido[2,3-b]indole in a human-flora associated mouse model. | consumption of probiotic bacteria such as bifidobacteria has been shown to reduce the risk of colon cancer in animal models. however, the composition and metabolic activities of the intestinal flora of experimental animals are significantly different from those of humans. the aim of the study was to examine whether the probiotic mixture, which consisted of streptococcus faecalis, clostridium butyricum and bacillus mesentericus, could decrease dna adduct formation induced by 2-amino-9h-pyrido[2,3 ... | 2003 | 12671533 |
| photocatalytic activity, antibacterial effect, and photoinduced hydrophilicity of tio2 films coated on a stainless steel substrate. | transparent tio2 films on stainless steel prepared by dip coating in a nonionic microemulsions solution have been shown to have much higher photocatalytic activity than those coatings on glass. fe3+ and fe2+ ions, diffusing from stainless steel substrate into tio2 films during high-temperature calcination, behave as dopants to significantly affect the films' photocatalytic activity. an optimum calcination condition, under which the amount of diffused fe3+ and the ratio of fe3+ to fe2+ ions favor ... | 2003 | 12785540 |
| molecular detection of a bacterial contaminant bacillus pumilus in symptomless potato plant tissue cultures. | an aberrant random amplified polymorphic dna (rapd) marker in genomic dna of tissue culture plantlets was frequently observed during a comparison of dna fingerprints derived from potato germplasm grown in tissue culture and the field. the rapd marker was cloned, sequenced and determined to be of bacterial origin. a bacterial contaminant was isolated from the tissue culture plants and identified as a bacillus pumilus. a set of sequence characterised amplified region (scar) primers were designed f ... | 2003 | 12789528 |
| effect of feed gas composition of gas discharge plasmas on bacillus pumilus spore mortality. | to investigate the effect of gas composition on the sensitivity of bacillus pumilus spores to gas plasmas. | 2003 | 12803552 |
| biofilm-specific cross-species induction of antimicrobial compounds in bacilli. | an air-membrane surface (ams) bioreactor was designed to allow bacteria to grow attached to a surface as a biofilm in contact with air. when bacillus licheniformis strain ei-34-6, isolated from the surface of a marine alga, was grown in this reactor, cells produced antimicrobial compounds which they did not produce when they were grown in shake flask cultures. an unidentified red pigment was also produced by surface-grown cells but not by planktonically grown cells. glycerol and ferric iron were ... | 2003 | 12839737 |
| poly(gamma-d-glutamic acid) protein conjugates induce igg antibodies in mice to the capsule of bacillus anthracis: a potential addition to the anthrax vaccine. | both the protective antigen (pa) and the poly(gamma-d-glutamic acid) capsule (gamma dpga) are essential for the virulence of bacillus anthracis. a critical level of vaccine-induced igg anti-pa confers immunity to anthrax, but there is no information about the protective action of igg anti-gamma dpga. because the number of spores presented by bioterrorists might be greater than encountered in nature, we sought to induce capsular antibodies to expand the immunity conferred by available anthrax vac ... | 2003 | 12857944 |
| the microbial ecology of cocoa bean fermentations in indonesia. | cocoa beans are the principal raw material of chocolate manufacture. the beans are subject to a microbial fermentation as the first stage in chocolate production. the microbial ecology of bean fermentation (forastero and trinitario cultivars) was investigated at three commercial fermentaries in east java, indonesia by determining the populations of individual species at 12-h intervals throughout the process. the first 2-3 days of fermentation were characterised by the successional growth of vari ... | 2003 | 12892924 |
| cynd, the cyanide dihydratase from bacillus pumilus: gene cloning and structural studies. | the cyanide dihydratase in bacillus pumilus was shown to be an 18-subunit spiral structure by three-dimensional reconstruction of electron micrographs of negatively stained material at its optimum ph, 8.0. at ph 5.4, the subunits rearrange to form an extended left-handed helix. gel electrophoresis of glutaraldehyde cross-linked enzyme suggests that the fundamental component of the spiral is a dimer of the 37-kda subunit. the gene was cloned, and the recombinant enzyme was readily expressed at hi ... | 2003 | 12902273 |
| adsorption of halogenated aromatic pollutants by a protein released from bacillus pumilus. | previous studies of the biosorption of halogenated aromatic pollutants (haps) have focused on the sorption of these compounds by cell bodies. however, in this study we investigated the adsorption of haps by biocompounds released from a bacterium, bacillus pumilus. when b. pumilus was exposed to high temperature, it released a protein and carbohydrates, exclusively. after determining experimental conditions using 1,2,3,4-tetrachlorinated dibenzofuran (1,2,3,4-tcdf), the adsorption characteristics ... | 2003 | 12909120 |
| degradation of african locust bean oil by bacillus subtilis and bacillus pumilus isolated from soumbala, a fermented african locust bean condiment. | to investigate predominant isolates of bacillus subtilis and b. pumilus in soumbala, a fermented african locust bean condiment, for their ability to degrade african locust bean oil (albo). | 2003 | 12969303 |
| antimicrobial activity of crude extracts from plant parts and corresponding calli of bixa orellana l. | ethanol extracts from the different parts of b. orellana showed differential antimicrobial activity. it was found that the extracts of in vitro leaves showed maximum activity against bacillus pumilus followed by the extracts from the roots and hypocotyls. the callus derived from different explants too showed antimicrobial activity. the leaf callus showed maximum activity. the zone of inhibition for the diluted extracts of in vitro hypocotyls and roots and their corresponding calli showed minimum ... | 2002 | 12974400 |
| [treatment of diarrheas with filtrates and lysates of bacillus subtilis, bacillus brevis and bacillus mesentericus]. | 1952 | 12992770 | |
| [studies on tetaine, a new antibiotic from theta strain of bacillus pumilus]. | 1952 | 13009425 | |
| the international standard for aureomycin. | in 1950, the department of biological standards, national institute for medical research, london, was authorized by the who expert committee on biological standardization to proceed with the establishment of an international standard for aureomycin. a 100-g batch of aureomycin was obtained and was compared with the standard preparation of aureomycin of the united states food and drug administration (fda) in a collaborative assay in which six laboratories in five countries participated.in all, 30 ... | 1953 | 13141137 |
| [isolation of tetaine, an antibiotic from the strain of bacillus pumilus]. | 1953 | 13182578 | |
| microbial pentosanases. ii. some factors affecting the production of pentosanases by bacillus pumilus and bacillus subtilis. | 1956 | 13293582 | |
| influence of potato dialysate on the production of tetain and glucose uptake by various strains of bacillus pumilus (teta). | 1962 | 14031643 | |
| pumilin, a new antibiotic from bacillus pumilus. | 1955 | 14370226 | |
| [phytopathogenic properties of bacillus mesentericus vulgatus]. | 1954 | 14383339 | |
| comparison of the final metabolism of strains of bacillus pumilus teta with different antibiotic activity. | 1962 | 14454766 | |
| extreme spore uv resistance of bacillus pumilus isolates obtained from an ultraclean spacecraft assembly facility. | recent environmental microbial sampling of the ultraclean spacecraft assembly facility at nasa jet propulsion laboratory (jpl-saf) identified spores of bacillus pumilus as major culturable bacterial contaminants found on and around spacecraft. as part of an effort to assess the efficacy of various spacecraft sterilants, purified spores of 10 jpl-saf b. pumilus isolates were subjected to 254-nm uv and their uv resistance was compared to spores of standard b. subtilis biodosimetry strains. spores ... | 2004 | 14502417 |
| survival and colonization of rhizobacteria in a tomato transplant system. | plant-growth-promoting rhizobacteria (pgpr) are used on crops most often as seed treatments; however, an alternative application method for transplanted vegetables is mixing pgpr into the soilless medium in which the transplants are grown. studies were undertaken to compare root colonization and persistence of rifampicin-resistant mutants of pgpr strains bacillus pumilus se34 and pseudomonas fluorescens 89b61, se34r and 89b61r, on tomato as a function of application method. when the bacteria wer ... | 2003 | 14569292 |
| diglucosyl-glycerolipids from the marine sponge-associated bacillus pumilus strain aas3: their production, enzymatic modification and properties. | the marine strain bacillus pumilus strain aas3, isolated from the mediterranean sponge acanthella acuta, produced a diglucosyl-glycerolipid, 1,2-o-diacyl-3-[beta-glucopyranosyl-(1-6)-beta-glucopyranosyl)]glycerol, with 14-methylhexadecanoic acid and 12-methyltetradecanoic acid as the main fatty acid moieties (ggl11). on a 30 l scale, using artificial seawater supplemented with glucose (20 g/l), yeast extract (10 g/l), and suitable nitrogen/phosphate sources, growth-associated glycoglycerolipid p ... | 2004 | 14593508 |
| antimicrobial activity of gymnema sylvestre leaf extract. | the ethanolic extract of gymnema sylvestre leaves demonstrated antimicrobial activity against bacillus pumilis, b. subtilis, pseudomonas aeruginosa and staphylococcus aureus and inactivity against proteus vulgaris and escherichia coli. | 2003 | 14630178 |
| genotyping of starter cultures of bacillus subtilis and bacillus pumilus for fermentation of african locust bean (parkia biglobosa) to produce soumbala. | bacillus spp. are the predominant microorganisms in fermented african locust bean called soumbala in burkina faso. ten strains selected as potential starter cultures were characterised by pcr amplification of the16s-23s rdna intergenic transcribed spacer (its-pcr), restriction fragment length polymorphism of the its-pcr (its-pcr rflp), pulsed field gel electrophoresis (pfge) and sequencing of the 968-1401 region of the 16s rdna. in previous studies, the isolates were identified by phenotyping as ... | 2004 | 14698101 |
| [amino acid composition of the capsular substance of anthrax bacilli and serologically identical specific substance of b. mesentericus]. | 1950 | 14793534 | |
| [effect of gramicidin c on the morphology and physiology of microorganisms; effect of gramicidin c on the hemolytic function of staphylococci and bacillus mesentericus]. | 1951 | 14833281 | |
| [chemical nature of antibiotics excreted by bacillus mesentericus]. | 1952 | 14940718 | |
| [bacteria of the bacillus mesentericus group as antagonist of actinomycetes]. | 1952 | 14940719 | |
| antifouling potential of some marine organisms from india against species of bacillus and pseudomonas. | crude methanolic extracts of 37 marine organisms (16 species of flora, 21 species of fauna) were screened for antibacterial properties against 5 strains of bacteria isolated from marine environments. of these, 10 plant and 9 animal extracts exhibited antibacterial activity against at least one bacterial strain. the extracts of 6 species were active against all the strains: i.e., stoechospermum marginatum (brown algae), cymodocea rotundata (seagrass), petrosia sp. and psammaplysilla purpurea (spo ... | 2002 | 14961269 |
| characterization of surfactin-like cyclic depsipeptides synthesized by bacillus pumilus from ascidian halocynthia aurantium. | a marine bacterium (kmm 1364), identified as bacillus pumilus, was isolated from the surface of ascidian halocynthia aurantium. structural analysis revealed that the strain kmm 1364 produced a mixture of lipopeptide surfactin analogs with major components with molecular masses of 1035, 1049, 1063, and 1077. the variation in molecular weight represents changes in the number of methylene groups in the lipid and/or peptide portions of the compounds. structurally, these lipopeptides differ from surf ... | 2002 | 14961278 |
| spore uv and acceleration resistance of endolithic bacillus pumilus and bacillus subtilis isolates obtained from sonoran desert basalt: implications for lithopanspermia. | bacterial spores have been used as model systems for studying the theory of interplanetary transport of life by natural processes such as asteroidal or cometary impacts (i.e., lithopanspermia). because current spallation theory predicts that near-surface rocks are ideal candidates for planetary ejection and surface basalts are widely distributed throughout the rocky planets, we isolated spore-forming bacteria from the interior of near-subsurface basalt rocks collected in the sonoran desert near ... | 2003 | 14987476 |
| use of bacteriocin promoters for gene expression in lactobacillus plantarum c11. | to exploit promoters involved in production of the bacteriocin sakacin p for regulated overexpression of genes in lactobacillus plantarum c11. | 2004 | 15012821 |
| n-acyl derivatives of asn, new bacterial n-acyl d-amino acids with surfactant activity. | new n-acyl d-amino acids were isolated from bacillus pumilus im 1801. their structures were determined by chemical analysis and mass spectrometry. the lipid part was identified as a mixture of fatty acids with 11, 12, 13, 15, and 16 carbon atoms in the iso, anteiso or n configuration linked by an amide bond with a d-asparagine. they exhibited surfactant properties. | 2004 | 15042452 |
| testing two lactobacillus plantarum and lactobacillus acidophilus strains for their suitability as a lipoid probiotic. | two strains of lactobacilli (lactobacillus acidophilus t-135 and lactobacillus plantarum 4/97) were selected in order to study their inhibitory properties against frequent udder pathogens (escherichia coli, staphylococcus aureus, streptococcus agalactiae, streptococcus uberis, salmonella enteritidis and bacillus pumilus), their production of organic acids as well as their ability to survive on the teat skin, the teat duct mucosa and in a lipoid emulsion. both strains inhibited the tested pathoge ... | 2004 | 15046462 |
| effect of terminal sterilization by irradiation on amber type i and type iii glass containers containing veterinary oxytetracycline suspension. | the purpose of this study is to determine the effect of terminal sterilization by gamma irradiation on 500-ml amber type i and type iii glass containers (bottles) containing oxytetracycline (otc) (25% w/v) suspension formulated using 20% (w/v) phospholipids syrup. the formulation was developed for veterinary parenteral administration. the results of terminal sterilization were used to assess the acceptability for this suspension product. otc is light-sensitive and needs to be stored in amber gla ... | 2004 | 15053050 |
| surfactant activity of a naphthalene degrading bacillus pumilus strain isolated from oil sludge. | we studied the growth, biosurfactant activities and petroleum hydrocarbon compounds utilisation of strain 28-11 isolated from a solid waste oil. the isolate was identified as bacillus pumilus. it grew well in the presence of 0.1% (w/v) of crude oil and naphthalene under aerobic conditions and utilised these substances as carbon and energy source. the capacity of strain 28-11 to emulsify crude oil and its ability to remove hydrocarbons looks promising for its application in environmental technolo ... | 2004 | 15066763 |
| characterization of bacillus probiotics available for human use. | bacillus species (bacillus cereus, bacillus clausii, bacillus pumilus) carried in five commercial probiotic products consisting of bacterial spores were characterized for potential attributes (colonization, immunostimulation, and antimicrobial activity) that could account for their claimed probiotic properties. three b. cereus strains were shown to persist in the mouse gastrointestinal tract for up to 18 days postadministration, demonstrating that these organisms have some ability to colonize. s ... | 2004 | 15066809 |
| growth promotion of red pepper plug seedlings and the production of gibberellins by bacillus cereus, bacillus macroides and bacillus pumilus. | the growth of red pepper plug seedlings was promoted by bacillus cereus mj-1, b. macroides cj-29, and b. pumilus cj-69 isolated from the rhizosphere. gibberellins (gas), a well-known plant growth-promoting hormone, were detected in the culture broth of their rhizobacteria. among the gas, the contents of ga1, ga3, ga4, and ga7, physiologically active gas, were comparatively higher than those of others, suggesting that the growth promoting effect was originated from the gas. this isthe first repor ... | 2004 | 15127789 |
| synthesis of aminoshikimic acid. | 5-amino-5-deoxyshikimic acid (aminoshikimic acid) was synthesized from glucose using recombinant amycolatopsis mediterranei and also synthesized by a tandem, two-microbe route employing bacillus pumilus and recombinant escherichia coli. | 2004 | 15128242 |
| inhibition of the growth of ascosphaera apis by bacillus and paenibacillus strains isolated from honey. | the fungus ascosphaera apis, the causative agent of chalkbrood disease in honeybee larvae, occurs throughout the world and is found in many beekeeping areas of argentina. the potential as biocontrol agents of 249 aerobic spore-forming bacterial antagonists isolated from honey samples was evaluated. each isolate was screened against a. apis by a central disk test assay. ten bacterial strains that showed the best antagonistic effect to a. apis were selected for further study and identified as baci ... | 2004 | 15174751 |
| maldi-tofms compared with other polyphasic taxonomy approaches for the identification and classification of bacillus pumilus spores. | to verify the efficacy of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tofms) protein profiling for identifying and differentiating bacterial species, several strains of bacillus pumilus were examined in a thorough taxonomic study incorporating a polyphasic approach. sixteen isolates of putative b. pumilus isolated from spacecraft assembly facilities, the mars odyssey spacecraft, and the international space station, were characterized for their biochemical ... | 2004 | 15177898 |
| isolation, characterization, and identification of bacterial contaminants in semifinal gelatin extracts. | bacterial contamination of gelatin is of great concern. indeed, this animal colloid has many industrial applications, mainly in food and pharmaceutical products. in a previous study (e. de clerck and p. de vos, syst. appl. microbiol. 25:611-618), contamination of a gelatin production process with a variety of gram-positive and gram-negative bacteria was demonstrated. in this study, bacterial contamination of semifinal gelatin extracts from several production plants was examined. since these extr ... | 2004 | 15184171 |
| isolation of bacillus pumilus from in vitro grapes as a long-term alcohol-surviving and rhizogenesis inducing covert endophyte. | to isolate and characterize the alcohol-surviving covert bacterium associated with grape tissue culture. | 2004 | 15186448 |
| [characterization of enterobacteria producing the low-molecular-weight antibiotics microcins]. | a comparative study of the morphological, cultural, physiological, and biochemical properties of the microcinogenic strains ecs 5/98, ecs 6/98, and ecb 214/99 with the known microcin c51 producer escherichia coli m17(p74) showed that these strains belong to the species e. coli. the strains produced microcins with molecular masses lower than 10 kda. microcin biosynthesis was stimulated by a deficiency of nutrients in the cultivation media. microcins were found to be resistant to thermolysin, but ... | 2004 | 15198029 |
| plant growth-promoting rhizobacteria systemically protect arabidopsis thaliana against cucumber mosaic virus by a salicylic acid and npr1-independent and jasmonic acid-dependent signaling pathway. | arabidopsis thaliana ecotype columbia plants (col-0) treated with plant growth-promoting rhizobacteria (pgpr) serattia marcescens strain 90-166 and bacillus pumilus strain se34 had significantly reduced symptom severity by cucumber mosaic virus (cmv). in some cases, cmv accumulation was also significantly reduced in systemically infected leaves. the signal transduction pathway(s) associated with induced resistance against cmv by strain 90-166 was determined using mutant strains and transgenic an ... | 2004 | 15255867 |
| bme585 i [5'-cccgc(4/6)-3'], a new isoschizomer of restriction endonuclease fau i, isolated from a strain of bacillus mesentericus. | bme585 i is a new member of the restriction endonuclease type iis family. it was partially purified from the heterothrophic, mesophilic bacterial strain bacillus mesentericus 585 by ammonium sulphate precipitation and phosphocellulose column chromatography. bme585 i is a monomeric protein with a molecular mass of 62 kd. the enzyme is active over a broad ph range from 7.0 to 8.8, has a temperature optimum of 37 degrees c and tolerance of nacl in reaction buffer from 0 to 400 mm. bme585 i recogniz ... | 2004 | 15293946 |
| evaluation of antibacterial properties of some medicinal plants used in iran. | forty-five species of 29 plant families used in the traditional medicine by iranian people, showed antibacterial activities against one or more of the bacterial species: bacillus cereus, bacillus pumilus, bordetella bronchiseptica, escherichia coli, klebsiella pneumoniae, micrococcus luteus, pseudomonas aeruginosa, pseudomonas fluorescens, serratia marcescens, staphylococcus aureus and staphylococcus epidermidis. no plant showed activity against serratia marcescens; bordetella bronchiseptica bei ... | 2004 | 15325735 |
| genotyping and toxigenic potential of bacillus subtilis and bacillus pumilus strains occurring in industrial and artisanal cured sausages. | artisanal and industrial sausages were analyzed for their aerobic, heat-resistant microflora to assess whether new emerging pathogens could be present among bacillus strains naturally contaminating cured meat products. sixty-four isolates were characterized by randomly amplified polymorphic dna (rapd)-pcr and fluorescent amplified fragment length polymorphism (faflp). the biotypes, identified by partial 16s rrna gene sequence analysis, belonged to bacillus subtilis, bacillus pumilus, and bacillu ... | 2004 | 15345396 |
| [isolation of endophytic bacteria in potato and test of antagonistic action to bacterial ring rot of potato]. | in this study, two hundred and forty bacterial strains were isolated from inner tissue of potato tubers collected from datong, taiyuan and inner mongolia autonomous regions. on the basis of antagonistic examination in vitro, fifty and five bacteria strains were characterized for antagonistic bacteria to ring rot of potato. it was 22.9 percentage of all bacteria strains. the biggest radius of suppression circle was 13 mm. nine strains were chosen for their suppression of bacterial ring rot, black ... | 2002 | 15346992 |
| gene cloning and expression of an alkaline serine protease with dehairing function from bacillus pumilus. | a new gene (named ap gene) encoding an alkaline serine protease with dehairing function was cloned from bacillus pumilus un-31-c-42 and its nucleotide sequence was determined. the expression of ap gene was induced with iptg in escherichia coli after the mature protease region was cloned into pet15b and sds-page showed expressed product clearly, but no alkaline protease activity was detected. in order to express the ap gene in b. subtilis, a recombinant expression plasmid was constructed which co ... | 2004 | 15386098 |
| [infections caused by bacillus mesentericus and similar organisms]. | 1950 | 15424145 | |
| chemical structure, conjugation, and cross-reactivity of bacillus pumilus sh18 cell wall polysaccharide. | bacillus pumilus strain sh18 cell wall polysaccharide (cwp), cross-reactive with the capsular polysaccharide of haemophilus influenzae type b, was purified and its chemical structure was elucidated using fast atom bombardment mass spectrometry, nuclear magnetic resonance techniques, and sugar-specific degradation procedures. two major structures, 1,5-poly(ribitol phosphate) and 1,3-poly(glycerol phosphate), with the latter partially substituted by 2-acetamido-2-deoxy-alpha-galactopyranose (13%) ... | 2004 | 15466043 |
| effect of culture conditions on the achievable taxonomic resolution of raman spectroscopy disclosed by three bacillus species. | confocal micro-raman spectroscopy requires a minimum of sample handling and no reagents and allows fast identification of microorganisms. since it reflects the overall molecular composition of the cells, it provides much more information than classical, microbial analyses. however, since the molecular makeup of a cell depends on culture conditions, it can be argued that this will affect the reproducibility and discrimination ability of raman spectroscopy. we used bacillus cereus, bacillus pumilu ... | 2004 | 15516118 |
| molecular characterization of a beta-1,4-endoglucanase from an endophytic bacillus pumilus strain. | endophytes comprise mainly microorganisms that colonize inner plant tissues, often living with the host in a symbiotic manner. several ecological roles have been assigned to endophytic fungi and bacteria, such as antibiosis to phytopathogenic agents and plant growth promotion. nowadays, endophytes are viewed as a new source of genes, proteins and biochemical compounds that may be used to improve industrial processes. in this study, the gene egla was cloned from a citrus endophytic bacillus strai ... | 2005 | 15538558 |
| the production of recombinant aprp, an alkaline protease derived from bacillus pumilus tyo-67, by in vitro refolding of pro-enzyme fixed on a solid surface. | bacillus pumilus tyo-67 has been isolated from tofuyo, a traditional fermented food made from soybean milk in okinawa, japan. this bacterium secretes a soybean-milk-coagulating enzyme (smce), which can be applied for the production of processed foods from soybean milk. thus, an easy method of producing the recombinant enzyme was developed in this study. smce is an alkaline serine protease belonging to the subtilisin family; its candidate gene, aprp, which encodes a prepro-enzyme, was isolated in ... | 2004 | 15625326 |
| microbial indicator groups in acidic mining lakes. | the salt composition of 14 acidic lakes was analysed, and their microbial communities were compared by their 16s rdna-based single-strand conformation polymorphism (sscp) fingerprints. the lakes were grouped into three chemically distinct types, and operational taxonomic units (otus) were identified in the 16s rdna sscp fingerprints. discriminant analysis between these groups using a multivariate approach showed that not a single organism, but rather a set of seven otus (ferromicrobium acidophil ... | 2005 | 15643943 |
| feasibility of detection and identification of individual bioaerosols using laser-induced breakdown spectroscopy. | the detection and identification of individual bioaerosols using laser-induced breakdown spectroscopy (libs) is investigated using aerosolized bacillus spores. spores of bacillus atrophaeous, bacillus pumilus, and bacillus stearothemophilus were introduced into an aerosol flow stream in a prescribed manner such that single-particle libs detection was realized. bacillus spores were successfully detected based on the presence of the 393.4- and 396.9-nm calcium atomic emission lines. statistical an ... | 2005 | 15649064 |