Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| 1h-2-benzopyran-1-one derivatives, microbial products with pharmacological activity. relationship between structure and activity in 6-[[1(s)-(3(s),4- dihydro-8-hydroxy-1-oxo-1h-2-benzopyran-3-yl)-3-methylbutyl]-amino]-4(s), 5(s)-dihydroxy-6-oxo-3(s)-ammoniohexanoate. | in order to investigate the structural requirements for gastroprotective activity in 6-[[1(s)-(3(s),4-dihydro-8- hydroxy-1-oxo-1h-2-benzopyran-3-yl)-3-methylbutyl]amino]-4(s),5(s)-dihydroxy 6-oxo-3(s)-ammoniohexanoate [ai-77-b, 1], a product of bacillus pumilus ai-77, nine derivatives were prepared and then tested for protective activity against stress-induced ulcers in rats. neither the chromophore, [1-(3,4-dihydro-8-hydroxy-1-oxo-1h-2-benzopyran-3-yl)-3-methylbutyl]ammonium chloride (2), nor t ... | 1983 | 6620300 |
| antimicrobial properties of some plant extracts against bacteria. | plant extracts obtained from feronia limonia (leaves) xanthium strumarium (flowering twigs) and glossocardia bosvellia (leaves) were tested for their antimicrobial properties against certain bacterial species. feronia leaf extract was ineffective on bacillus pumilus and x. campestris, vibrio cholerae was found quite sensitive to this extract. the extract of x. strumarium showed an abnormality with v. cholerae, where the inhibition exceeded the control with established antibiotics. similarly, g. ... | 1983 | 6667916 |
| [several forms of bacillus mesentericus. iv. plasmid content]. | 1984 | 6741614 | |
| [isolation, purification and properties of bacillus mesentericus proteinases]. | 1982 | 6750327 | |
| binding of alkyl beta-d-xylopyranosides, containing branched-chain, cyclic, and substituted aglycon groups, to beta-d-xylosidase from bacillus pumilus prl b12. | for a number of alkyl beta-d-xylopyranosides having branched-chain, cyclic, and substituted aglycon groups, and binding to beta-d-xylosidase from b. pumilus prl b12, the binding constant ki and (for some of them) the thermodynamic equilibrium parameters delta h0, delta s0, and delta g0 have been determined. although the aglycon is bound through hydrophobic forces, no simple relationships between the binding parameters and the relative hydrophobicity of the alkyl beta-d-xylopyranosides could be d ... | 1980 | 6766356 |
| regulation of a glutamine amidotransferase subunit from bacillus subtilis by a cloned trpe gene from bacillus pumilus. | the influence of a cloned trpe (trpe+p) gene from bacillus pumilus on the expression of the gat locus in bacillus subtilis was examined. the trpe gene was regulated by tryptophan and the mtr locus, which specifies the presumed aporepressor. the specific activity of subunit g varied directly with the level of subunit ep, and the heterologous epg complex that was formed was stable to gel filtration. | 1980 | 6767699 |
| [infrared spectra of bacillus subtilis, bacillus mesentericus and bacillus brevis]. | 1980 | 6767901 | |
| synthesis of 4-(3-benzazolymethyl)aminodiphenyl ethers and other related products as potential antimicrobial agents. | 4-aminodiphenyl ethers (2) obtained by reducing the corresponding nitro analogue (1), have been incorporated into benzazoles, phthalimides and 4-quinazolone in the presence of 40% formalin to furnish a series of 4-(3-benzazolymethyl)aminodiphenyl ethers (3) under the conditions of the mannich reaction. in an alternate procedure, 3 were obtained on refluxing the n-hydroxymethyl derivatives of benzazoles etc. with 2 in methanol. a few amine exchange reactions were also performed and the stability ... | 1980 | 6770377 |
| dna-membrane association is necessary for initiation of chromosomal and plasmid replication in bacillus subtilis. | we examined the effect of the inhibition of initiation of dna replication on the membrane association of the chromosomal origin of replication of bacillus subtilis and the staphylococcus aureus-bacillus pumilus chimeric plasmid psl103, using temperature-sensitive mutants of b. subtilis that have specifically affected initiation. inhibition of initiation of the chromosome and psl103 in the initiation mutant dna-1 results in a decrease in the membrane association of both a marker near the chromoso ... | 1980 | 6771760 |
| [extracellular proteases, growth phases and sporulation in the cultivation of bacillus mesentericus strain 76]. | 1981 | 6803525 | |
| beta-d-xylosidase from bacillus pumilus. | 1982 | 6808311 | |
| [biosynthesis, isolation and various characteristics of pigments from the r form of bacillus mesentericus strain 90]. | 1982 | 6820615 | |
| antimicrobial activity of 3-methyl-5-furfurylidene-2-thioxo-4-thiazolidones. | substituted 3-methyl-5-furfurylidene-2-thioxo-4-thiazolidones 1-17 were tested for their action against bacillus pumilus, b. brevis and b. megaterium and against aspergillus flavus, a. fumigatus and a. niger. most of the compounds possessed an activity against both bacteria and fungi. these compounds were also tested for their effectiveness as virus inhibitors employing nicotiana glutinosa as assay host against tobacco mosaic virus. most of them exhibited potential antiviral activity both in vit ... | 1983 | 6832657 |
| aerobic mesophilic and psychrotrophic sporeforming bacteria in buffalo milk. | seasonal variation of the population of aerobic sporeformers in raw milk was higher in summer than in other seasons. least variation was in fall, but variation in winter and spring was similar. aerobic mesophilic sporeformers in raw milk consisted mainly of bacillus subtilis (42.5%) and bacillus megaterium (34.8%), followed by bacillus circulans (4.9%), bacillus cereus (4.6%), bacillus pumilus (2.9%), bacillus polymyxa (2.8%), bacillus licheniformis (1.9%), bacillus badius (1.5%), bacillus brevi ... | 1983 | 6886164 |
| preparation and some chemical characteristics of milk-clotting protease from bacillus mesentericus 76. | pure milk-clotting protease (mcp-76) is isolated by isotachophoresis at ph 5.0. the native molecule has only one protein chain. it is a metaloenzyme containing zinc. the pure mcp-76 has a molecular weight of 33 000 (+/- 1500) and by diphenyl-indenonyl-isothiocyanate method showed arginine as n-terminal amino acid. | 1981 | 7014483 |
| bacillus pumilus in the induction of clindamycin-associated enterocolitis in guinea pigs. | antibiotic-associated enterocolitis was induced in guinea pigs by the intraperitoneal injection of clindamycin. the colonic and cecal mucosa and feces of acutely ill animals were cultured under aerobic and anaerobic conditions on 5% sheep blood agar plates and on a selective and differential medium for clostridium difficile. all morphologically distinct colony types were isolated in pure culture and identified. a sterile cell-free filtrate of each isolate was tested for ability to induce morphol ... | 1982 | 7033138 |
| a novel extracellular proteinase from bacillus pumilus. | bacillus pumilus nctc aii6/73 produces a novel extracellular alkaline proteinase whose optimum is ph 9.5. the purified product from ion-exchange chromatography (deae-cellulose) was resolved into two bands by polyacrylamide-sodium dodecyl sulphate (sds) gel electrophoresis. these had approximate molecular weights of 15.6 x 10(3) daltons, respectively, and a high content of glycine and proline, comprising roughly 50% of the total amino residues. | 1981 | 7034386 |
| [forms of bacillus mesentericus. iii. ultrastructural studies under conditions for proteolytic enzyme biosynthesis]. | 1981 | 7048855 | |
| the radiation-induced inactivation of microorganisms in non-aqueous suspension: the effect of selected alcohols and paraffins on the radiation sensitivity of aerated bacillus pumilus spores. | the effect of model compounds comprising alcohols and paraffins on the radiation sensitivity of b pumilus spores has been carried out with the aim of understanding the radiation-induced inactivation of microorganisms when suspended in non-aqueous medium. this study is a prerequisite to the undertaking of radiation sterilization of non-aqueous pharmaceuticals. spores of b. pumilus e601 mounted on kaolin powder were suspended in the appropriate organic agent and gamma irradiated under oxic conditi ... | 1981 | 7244117 |
| [the use of biological indicators in radiosterilisation: i. bacillus pumilus e 601 (author's transl)]. | 1981 | 7247236 | |
| [changes in bacillus pumilus antibiotic activity and sensitivity with integration and disintegration of plasmid ppl 7065]. | the role of plasmid ppl 7065 in the control of the antibiotic production and sensitivity in bac. pumilus was studied with the use a plasmid-free strain and strains containing the plasmid in the cells in a free or chromosome-integrated state. it was found that all the strains had antibiotic activity only with respect to a limited number of gram-positive bacteria. still, the antibiotics produced by them differed in the antibacterial spectrum. the antibiotic produced by the plasmid-free strain had ... | 1981 | 7305314 |
| enzymic formation of difructose anhydride iv from bacterial levan. | a novel enzyme for producing difructose anhydride iv (di-d-fructofuranose 2,6' : 6,2' dianhydride) from levan of bacillus mesentericus was prepared from a culture of arthrobacter ureafaciens, and the conditions and method to determine the enzymatic activity were determined. levan of bacillus subtilis also yielded the difructose dianhydride, but inulin, inulobiose, sucrose, levanbiose, and levantriose did not produce it on enzymic reaction under similar test conditions. | 1981 | 7338521 |
| binding of n-alkyl beta-d-xylopyranosides and n-alkyl 1-thio-beta-d-xylopyranosides to beta-d-xylosidase from bacillus pumilus prl b12. | the binding of d-xylose and of a series of n-alkyl beta-d-xylopyranosides and their 1-thio analogues to beta-d-xylosidase from b. pumilus prl b12 has been investigated. the binding constants and thermodynamic equilibrium parameters delta h0 and delta s0 have been determined. the enzyme does not distinguish between alpha- and beta-d-xylopyranose. although the enthalpy of binding of d-xylose is very favourable, the overall free-energy is small, due to a large decrease in entropy. furthermore, all ... | 1980 | 7351026 |
| [integrative suppression of antibiotic formation in bacillus pumilus]. | relationship between antibiotic production and ppl 7065 plasmid integration into the chromosome of b. pumilus atcc 7065-k was studied. with this purpose hybridization of plasmid dna isolated from strain atcc 7065 producing no antibiotic was performed with chromosome dna of b. pumilus atcc 7065 and w20, as well as b. subtilis and e. coli k-12. plasmid dna labeled with 3h was used in the experiments. it was found to be slightly bound with dna of b. subtilis (0.8 per cent), e. coli k-12 (0.2 per ce ... | 1980 | 7362219 |
| resistance and recovery studies on ultraviolet-irradiated spores of bacillus pumilus. | a spore suspension model and a procedure for recovering ultraviolet (uv)-irradiated spores of bacillus pumilus were investigated. a most-probable-number tube dilution method using double-strength trypticase soy broth was found to be superior to the agar plate method for recovering optimal numbers of spores irradiated with sublethal doses of uv energy. aqueous suspensions of b. pumilus survived uv doses up to 108,000 ergs/mm2 as determined by a most-probable-number recovery and estimation procedu ... | 1980 | 7377772 |
| purification and properties of polygalacturonic acid trans-eliminase from bacillus stearothermophilus. | a strain of thermophilic bacteria, bacillus stearothermophilus, with pectolytic activity has been isolated. it produced an endo-polygalacturonic acid trans-eliminase (endo-pate, ec 4.2.2.2) extracellularly when grown at 65 degrees c on a pectic acid medium. the pate was purified 62-fold by the rapid affinity chromatographic method on a sepharose-polygalacturonamide linked matrix. the absorbed pate was eluted from the column with a continuous gradient of 0-10(-3)m ethylenediaminetetraacetic acid ... | 1980 | 7407714 |
| [physicochemical properties and biological activity of an antitumor substance isolated from bacillus mesentericus culture broth]. | 1980 | 7464577 | |
| comparative effects of gamma rays and electron beams on spores of bacillus pumilus. | the effects of gamma rays and electron beams on the germination, outgrowth and the synthesis of protein and rna of bacillus pumilus spores were investigated to clarify the difference in the effects of the two types of radiations on bacterial spores. gamma irradiation facilitated the germination to a slightly larger degree than electron irradiation. the outgrowth, growth and the synthesis of protein and rna were inhibited by gamma irradiation to a greater extent than electron irradiation, when th ... | 1994 | 7510894 |
| machine operator's lung. a hypersensitivity pneumonitis disorder associated with exposure to metalworking fluid aerosols. | six auto parts manufacturing workers were referred for evaluation of a 6-week history of work-related dyspnea, cough, and fatigue. two workers also reported fever and weight loss. all six worked in a machining area where a waterbased metalworking fluid was used and recirculated under high pressure, thereby creating an aerosol. chest radiographs revealed pulmonary interstitial infiltrates in four workers. lung function tests showed that four workers had decreased diffusing capacity. after removal ... | 1995 | 7656609 |
| antibiotic production and biocontrol activity by bacillus subtilis cl27 and bacillus pumilus cl45. | bacillus subtilis cl27 and b. pumilus cl45 showed similar activity against botrytis cinerea in in vitro plate assays. in a seedling bioassay, however, b. subtilis cl27 had activity similar to a commercial fungicide while b. pumilus cl45 failed completely to prevent seedling damping-off caused by bot. cinerea. antibiotic production by the two bacillus strains was found to depend on the growth substrate and highest antibiotic production was found on media based on homogenized cabbage tissue. antib ... | 1995 | 7698955 |
| [biosynthesis of extracellular ribonuclease by bacillus pumilus]. | conditions for the biosynthesis of alkaline extracellular ribonuclease by bacillus pumilus were studied and their comparison with that of the biosynthesis of ribonuclease by bacillus intermedius was made. it was estimated that ribonuclease of bacillus pumilus is synthesized post-exponentially, its synthesis is inhibited by inorganic phosphate and is stimulated by actinomycin d, as the synthesis of ribonuclease by bacillus intermedius. at the same time essential differences in dynamic of enzyme f ... | 1994 | 7760766 |
| nucleotide sequence of the clostridium stercorarium xyna gene encoding xylanase a: identification of catalytic and cellulose binding domains. | the nucleotides of the xyna gene of clostridium stercorarium were sequenced. the structural gene consists of an open reading frame of 1533 bp encoding 511 amino acids with an m(r) of 56,519. the signal peptide cleavage site was identified by comparison with the n-terminal amino acid sequence of the enzyme produced by a recombinant escherichia coli. xylanase a consists of a catalytic domain belonging to family g at the n-terminus and two direct repeats of about 90 amino acids with a short spacing ... | 1993 | 7763496 |
| phenol and cresol metabolism in bacillus pumilus isolated from contaminated groundwater. | from an aquifier contaminated with phenolic compounds seven bacterial strains able to grow on phenol and several mono- and disubstituted alkylphenols as sole source of carbon and energy were isolated. five isolates belong to the genus pseudomonas, two to the genus bacillus. the isolate most active in utilization of the applied xenobiotics was identified as bacillus pumilus and used for the investigation of the degradation pathways in liquid cultures. cells of this strain precultured on phenol we ... | 1995 | 7783002 |
| disinfection efficacy in an integrated ultraviolet light contact lens care system. | we evaluated a new integrated contact lens care system that combines fluid turbulence for lens cleaning with ultraviolet (uv) light for solution sterilization. the ultraviolet light system was used to clean and disinfect 42 soft contact lenses (water contents: 38.6%, 43%, 55%, and 70%) and two rigid gas permeable lenses. test lenses were contaminated with 10(6) cells/ml of bacillus pumilus, aspergillus niger, pseudomonas aeruginosa, and acanthamoeba castellanii and subjected to a 15-minute clean ... | 1994 | 7820920 |
| the mtrb gene of bacillus pumilus encodes a protein with sequence and functional homology to the trp rna-binding attenuation protein (trap) of bacillus subtilis. | the mtrb gene from bacillus pumilus encodes a 76-amino-acid polypeptide with 77% identity to the trp rna-binding attenuation protein (trap) from bacillus subtilis. b. pumilus trap binds trp leader rna from either b. subtilis or b. pumilus in a tryptophan-dependent manner. altering threonine 52 to alanine eliminated rna-binding activity of b. pumilus trap. | 1995 | 7836324 |
| gamma sterilization of a semi-solid poly(ortho ester) designed for controlled drug delivery--validation and radiation effects. | radiation sterilization is becoming increasingly popular for the sterilization of many pharmaceutical products. although this technique is not limited to the sterilization of polymers, it is probably the most suitable method for such materials. this method however suffers several drawbacks. the sterilization of a product must lead to a safety level of 10(-6), i.e. one chance in a million to find a contaminated sample. in many cases, this assurance of sterility can be achieved by using a uniform ... | 1994 | 7855057 |
| purification and characterization of ferulate and p-coumarate decarboxylase from bacillus pumilus. | bacillus pumilus ps213 isolated from bovine ruminal fluid was able to transform ferulic acid and p-coumaric acid to 4-vinylguaiacol and 4-vinylphenol, respectively, by nonoxidative decarboxylation. the enzyme responsible for this activity has been purified and characterized. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of crude extract from a culture induced by ferulic acid or p-coumaric acid shows three bands that are not present in the crude extract of an uninduced culture, while ... | 1995 | 7887611 |
| phosphate regulation of biosynthesis of extracellular rnases of endospore-forming bacteria. | the gene for the extracellular ribonuclease of b. pumilus kmm62 (rnase bp) has been cloned and sequenced. the structural gene for this enzyme is similar to those of the extracellular ribonucleases of b. intermedius 7p (binase) and b. amyloliquefaciens h2 (barnase), as are the regulatory regions of binase and rnase bp. the regulatory region of the barnase gene, however, is quite different from the other two. in the promoter of the genes for binase and rnase bp, but not in that for barnase, is a r ... | 1995 | 8001670 |
| bacillus dna in fossil bees: an ancient symbiosis? | we report here the isolation of dna from abdominal tissue of four extinct stingless bees (proplebeia dominicana) in dominican amber, pcr amplification of a 546-bp fragment of the 16s rrna gene from bacillus spp., and their corresponding nucleotide sequences. these sequences were used in basic local alignment search tool searches of nonredundant nucleic acid data bases, and the highest scores were obtained with 16s rrna sequences from bacillus spp. phylogenetic inference analysis by the maximum-l ... | 1994 | 8031102 |
| mechanism of inhibited growth of bacillus pumilus by propionibacterium freudenreichii subsp. shermanii. | physiological studies were conducted in an attempt to elucidate the mechanism of inhibition of bacillus pumilus by propionibacterium freudenreichii subsp. shermanii. inhibition of b. pumilus by p. shermanii occurred in media supplemented with 1% glucose, indicating that glucose utilization by the latter bacterium was not responsible for growth inhibition of the former bacterium. the medium ph in which p. shermanii inhibited the growth of b. pumilus was 4.3. propionic acid was positively identifi ... | 1994 | 8060789 |
| [h2o2 low temperature plasma sterilization. new possibilities for use with eye surgery instruments]. | the h2o2-low-temperature-plasma-sterilization (sterrad 100) works with a temperature below 50 degrees c (140 degrees f). this system is appliable for thermostabile materials as well as for thermolabile materials. the efficancy of this new system is shown by a biological test with bacillus pumilus spores. 5 typical ophthalmic surgical instruments were contaminated. after sterilization the numerical reduction of the microorganisms had to be at least 6 log levels. corrosion caused by hydrogene pero ... | 1993 | 8124049 |
| hydrolysis of glycosylpyridinium ions by anomeric-configuration-inverting glycosidases. | the hydrolyses of five beta-d-xylopyranosylpyridinium ions by the beta-d-xylosidase of bacillus pumilus proceed with kcat values 10(8)-10(9)-fold larger than the rates of spontaneous hydrolysis of the same compounds. log(kcat) values correlate well with aglycon pk(a) [b1g(v) = -0.52, r = 0.99], whereas the correlation of log(kcat/km) is poor [r = 0.77; beta 1g(v/k) = approximately -0.6]. the (1-->3)-beta-d-glucanase of sporotrichum dimorphosporum hydrolyses 4-bromo-2-(beta-d-glucopyranosyl)isoqu ... | 1993 | 8143292 |
| specificity of chitosanase from bacillus pumilus. | partially (25-35%) n-acetylated chitosan was digested by chitosanase from bacillus pumilus bn-262, and structures of the products, partially n-acetylated chitooligosaccharides, were analyzed in order to investigate the specificity of the chitosanase. the chitosanase produced glucosamine (glcn) oligosaccharides abundantly, indicating that the chitosanase splits the beta-1,4-glycosidic linkage of glcn-glcn. the chitosanase also produced hetero-oligosaccharides consisting of glucosamine and n-acety ... | 1994 | 8155696 |
| [metalloproteinase of bacillus mesentericus, strain v-313]. | a homogeneous metalloproteinase has been isolated with a 28% yield from the culture fluid of bacillus mesentericus, strain b-313. the isolation procedure included chromatography on bacitracin-silochrome and gel filtration on acrylex p-10 and sephadex g-75. the enzyme has a molecular mass of 41,000 da; its n-terminal sequence, which appears as a-a-t-t-g-t-g-t-t-l-k-g-k-t-v-s-l-n-i, is identical with that of the b. amyloliquefaciens enzyme. like other metalloproteinases, the enzyme is inhibited by ... | 1993 | 8218566 |
| the xync gene from fibrobacter succinogenes s85 codes for a xylanase with two similar catalytic domains. | the xync gene of fibrobacter succinogenes s85 codes for a 66.4-kda xylanase which consists of three distinct domains separated by two flexible regions rich in serine residues. domains a and b of xync code for catalytic domains with 56.5% identity and 9.6% similarity with each other, and both domains share homology with xylanases of ruminococcus flavefaciens, neocallimastix patriciarum, clostridium acetobutylicum, bacillus pumilus, bacillus subtilis, and bacillus circulans. more than 88% of the x ... | 1993 | 8244936 |
| interactive behavior of saccharomyces cerevisiae, bacillus pumilus and propionibacterium freudenreichii subsp. shermanii. | prevention of ropy bread caused by mucoid variants of certain bacilli presents a major problem for developing countries where cost of preservatives is prohibitive. control of ropiness may be achieved by using propionic acid-producing bacteria in mixed culture with leavening yeasts. therefore, interaction studies between propionibacterium freudenreichii subsp. shermanii, bacillus pumilus and saccharomyces cerevisiae were conducted in a chemically defined medium to test the relevance of such an ap ... | 1993 | 8257655 |
| purification and some properties of an alkaline xylanase from alkaliphilic bacillus sp. strain 41m-1. | an alkaliphilic bacillus sp. strain, 41m-1, isolated from soil produced multiple xylanases extracellularly. one of these xylanases was purified to homogeneity by ammonium sulfate fractionation and anion-exchange chromatography. the moleculr mass of this enzyme (xylanase j) was 36 kda, and the isoelectric point was ph 5.3. xylanase j was most active at ph 9.0. the optimum temperature for the activity at ph 9.0 was around 50 degrees c. the enzyme was stable up to 55 degrees c at ph 9.0 for 30 min. ... | 1993 | 8292206 |
| kinetic characterization of alkaline mesentericopeptidase. comparison with serine proteinases from different origins. | comparative studies of the hydrolysis of succinyl-ala2-phe-methylcoumarylamide with mesentericopeptidase, a mesophilic extracellular serine proteinase from bacillus mesentericus, and proteinases produced by organisms representing different levels of evolutionary development, were performed. drastic differences in the proteolytic coefficient kcat/km were found. as regards their catalytic efficiency, the proteinases studied can be placed in the following order: mesentericopeptidase < subtilisin no ... | 1993 | 8307687 |
| isolation and characterization of a cyanide dihydratase from bacillus pumilus c1. | a cyanide-degrading enzyme from bacillus pumilus c1 has been purified and characterized. this enzyme consisted of three polypeptides of 45.6, 44.6, and 41.2 kda; the molecular mass by gel filtration was 417 kda. electron microscopy revealed a multimeric, rod-shaped protein approximately 9 by 50 nm. cyanide was rapidly degraded to formate and ammonia. enzyme activity was optimal at 37 degrees c and ph 7.8 to 8.0. activity was enhanced by sc3+, cr3+, fe3+, and tb3+; enhancement was independent of ... | 1993 | 8407782 |
| a new antibacterial sesquiterpene from premna oligotricha. | a novel sesquiterpene, 7 alpha-hydroxy-6, 11-cyclofarnes-3(15)-en-2-one [1], has been isolated from the aerial parts of premna oligotricha (verbenaceae) using an antimicrobial bioassay-guided isolation procedure. the sesquiterpene was identified on the basis of spectroscopic data and showed weak activity against gram-positive bacteria bacillus pumilus, bacillus subtilis, staphylococcus aureus, and streptococcus faecalis. | 1993 | 8450316 |
| cloning, sequencing, and expression in escherichia coli of the bacillus pumilus gene for ferulic acid decarboxylase. | the bacillus pumilus gene encoding a ferulic acid decarboxylase (fdc) was identified and isolated by its ability to promote ferulic acid decarboxylation in escherichia coli dh5 alpha. the dna sequence of the fdc gene was determined, and the recombinant enzyme produced in e. coli was purified and characterized. | 1995 | 8534115 |
| [biosynthetic regulation of extracellular ribonucleases in native strains of bacilli and in recombinant strains of escherichia coli]. | regulation of the biosynthesis of extracellular ribonucleases of bacillus amyloliquefaciens h2 (barnase), bacillus intermedius 7p (binase), and bacillus pumilus kmm 62 (rnase bp) was studied in their native strains and recombinant escherichia coli strains. recombinant plasmids were obtained that contained genes encoding barnase, binase, and rnase bp under the control of their own regulatory sequences (plasmids pmt415, pml5, and pml61), genes encoding barnase and binase under the control of the t ... | 1995 | 8538511 |
| isolation of a gene essential for biosynthesis of the lipopeptide antibiotics plipastatin b1 and surfactin in bacillus subtilis yb8. | bacillus subtilis yb8 was found to produce the lipopeptide antibiotics surfactin and plipastatin b1. a gene, lpa-8, required for the production of both lipopeptides was cloned from strain yb8. when this gene was inactivated in strain yb8, neither surfactin nor plipastatin b1 was produced. however, the defective strain transformed with an intact lpa-8 gene had restored ability to produce both peptides. nucleotide sequence analysis of the region essential for the production of the peptides reveale ... | 1996 | 8639027 |
| a simple assay for xylanase using o-nitrophenyl-beta-d-xylobioside. | we measured xylanase activities upon eight chromogenic substrates, o- or p-nitrophenyl-beta-d-xylopyranoside (onp-x or pnp-x) and o- or p-nitrophenyl-beta-d-xylooligosaccharides (onp-xn or pnp-xn, n = 2-4), and studied for their uses as substrates in a kinetic study. the kcat and k(m) of bacillus pumilus xylanase (ec 3.2.1.8) activities for pnp-x2 were 0.24s-1 and 0.5 mm, respectively. the relative xylanase activities with the other substrates to that with pnp-x2, pnp-x3, pnp-x4, onp-x, onp-x2, ... | 1996 | 8695915 |
| note: microbial resistance of wool fabric treated with bis-quats compounds. | in this paper, the antibacterial activity against bacillus pumilus, staphylococcus aureus, escherichia coli and pseudomonas aeruginosa of the wool fabrics treated with new antimicrobial bis-quaternary surfactants, dabk and dabb, is studied. the activity was established on the basis of the agar diffusion and protective antibacterial test results and on the basis of scanning electron microscopy (sem) observation. the results were compared with the htab, a monoquaternary surfactant of conventional ... | 1996 | 8760331 |
| in vitro studies of the loss of antibacterial activity of oxytetracycline in presence of ca(ii) or mg(ii) ions. | the results of a comparative study, which evaluated the in vitro effect on the antibacterial activity of oxytetracycline (otc, cas 79-57-2) in presence of ca(ii)/mg(ii) ions suggest that susceptibility of staphylococcus aureus, bacillus pumilis and bacillus subtilis to otc is reduced in presence of ca(ii)/mg(ii) ions. as the ratio of concentration of ca(ii)/mg(ii) to otc was increased, antibacterial activity of otc declined. in addition to the difference observed between the antibacterial effect ... | 1996 | 8842342 |
| comparison of the properties of native and pentaammineruthenium(iii)-modified xylanase. | two xylanases, xyna of bacillus pumilus and xyn ii of trichoderma reesei, were purified and then modified by the attachment of pentaammineruthenium, thereby resulting in the generation of a xylanase with veratryl alcohol oxidase activity. hydrolytic activity of t. reesei xyn ii on soluble xylans was unchanged by modification with pentaammineruthenium; however, modification of b. pumilus xyna greatly reduced xylan hydrolysis unless the active site of the xylanase was protected with xylose during ... | 1996 | 8987538 |
| simple detection of xylosidase activity in single colonies, using 1-naphthyl-beta-d-xylopyranoside. | since the xylosidase of bacillus pumilus hydrolyzed 1-naphthyl-beta-d-xylopyranoside (naphthyl-x) to produce xylose and 1-naphthol and a chromogenic azo compound is produced by coupling 1-naphthol and fast blue salt b, a simple method for detection of xylosidase activity in single colonies was studied. escherichia coli jm109 carrying the xylosidase gene of b. pumilus was cultivated at 37 degrees c for 18 h on a lb plate containing 0.5 mg/ml naphthyl-x, and then the plate was overlaid with 3 ml o ... | 1996 | 8987608 |
| two related rolling circle replication plasmids from salt-tolerant bacteria. | in a range of salt-tolerant bacteria isolated from soil, rhizosphere, and phyloplane, cross-hybridization tests revealed a group of seven plasmids which were chosen for further study. two plasmids (psh1418 and psh1451) were picked as representative examples of the two related subgroups. restriction mapping and southern blotting identified a region common to the two plasmids which later was identified as encoding the replication functions. we were unable to join these plasmids to high-copy-number ... | 1996 | 9007014 |
| [effect of culture medium components on the accumulation of extracellular bacillary ribonucleases in culture fluid of recombinant strains of escherichia coli]. | the effect of the concentrations of peptone, yeast extract, and inorganic phosphate on the expression of genes of extracellular ribonucleases from bacillus intermedius 7p (binase) and bacillus amyloliquefaciens h2 (barnase) was studied in escherichia coli cells transformed with plasmids containing the structural genes of binase or barnase under the control of their own or synthetic regulatory region or the structural binase gene under the control of the regulatory regions of the genes of barnase ... | 1996 | 9102546 |
| leader peptides of inducible chloramphenicol resistance genes from gram-positive and gram-negative bacteria bind to yeast and archaea large subunit rrna. | cata86 is the second gene in a constitutively transcribed, two-gene operon cloned from bacillus pumilus . the region that intervenes between the upstream gene, termed the leader, and the cata86 coding sequence contains a pair of inverted repeat sequences which cause sequestration of the cata86 ribosome binding site in mrna secondary structure. as a consequence, the cata86 coding sequence is untranslatable in the absence of inducer. translation of the cata86 coding sequence is induced by chloramp ... | 1997 | 9108153 |
| cloning of the bacillus pumilus beta-xylosidase gene (xynb) and its expression in saccharomyces cerevisiae. | a genomic dna library of the bacterium bacillus pumilus pls was constructed and the beta-xylosidase gene (xynb) was amplified from a 3-kb genomic dna fragment with the aid of the polymerase chain reaction technique. the amplified xynb gene was inserted between the yeast alcohol dehydrogenase ii gene promoter (adh2p) and terminator (adh2t) sequences on a multicopy episomal plasmid (pdlg11). the xynb gene was also fused in-frame to the secretion signal sequence of the yeast mating pheromone alpha- ... | 1997 | 9114518 |
| purification and characterization of a dipeptidyl carboxypeptidase from pseudomonas sp. wo24. | a dipeptidyl carboxypeptidase (dcp) activity was detected in cell-free extracts of pseudomonas sp. wo24. after purification and characterization the enzyme was found to be homogeneous by sds-page, and had a molecular mass of 74,000 da by sds-page and 72,000 da by gel filtration, indicating that it is monomeric. the isoelectric point was 5.2 and optimum ph was 6.5-7.0. it showed a specific activity of 780 mumol/min/mg, which is the highest of the values shown by known enzymes. the enzyme hydrolyz ... | 1997 | 9178563 |
| investigation of pulsed light for terminal sterilization of wfi filled blow/fill/seal polyethylene containers. | a study was performed to assess the ability of pulsed light to sterilize water for injection in blow/fill/seal polyethylene containers. pulsed light uses intense, short duration flashes of broad spectrum white light to produce high levels of microbial kill. in a first phase of testing, containers of 0.5, 5, 15, and 120 ml nominal volume were inoculated with bacillus pumilus endospores, bacillus subtilus variety niger strain globigii endospores, bacillus stearothermophilus endospores, and aspergi ... | 1997 | 9203823 |
| effect of high-power microwave on indicator bacteria for sterilization. | according to the superiority sterilization, a specially-designed microwave disinfector using high-power microwave energy was made. a series of sterilizing experiments have been made to determine the effect of microwave energy on several typical indicator bacteria such as bacillus subtilis var. niger, bacillus stearothermophilus, bacillus pumilus e601, staphylococcus aureus, bacillus cereus. under the conditions of different sterilization duration and unequal intensity of microwave power irradiat ... | 1996 | 9216147 |
| cloning and expression of the glutamate racemase gene of bacillus pumilus. | a glutamate racemase gene (muri) was found in bacillus pumilus cells and cloned into escherichia coli wm335, a d-glutamate auxotroph, by means of a genetic complement method. muri of b. pumilus encodes a 272-amino acid protein with an unusual initiation codon, ttg. the deduced amino acid sequence shows significant similarity with those of glutamate racemases from e. coli (ratio of identical residues, 28%), pediococcus pentosaceus (44%), and staphylococcus haemolyticus (49%). b. pumilus muri was ... | 1997 | 9354391 |
| bpli, a new bcgi-like restriction endonuclease, which recognizes a symmetric sequence. | bcg i and bcg i-like restriction endonucleases cleave double stranded dna specifically on both sides of their asymmetric recognition sequences which are interrupted by several ambiguous base pairs. their heterosubunit structure, bifunctionality and stimulation by adomet make them different from other classified restriction enzymes. here we report on a new bcg i-like restriction endonuclease, bpl i from bacillus pumilus , which in contrast to all other bcg i-like enzymes, recognizes a symmetric i ... | 1997 | 9358150 |
| cloning and analysis of the four genes coding for bpu10i restriction-modification enzymes. | the bpu 10i r-m system from bacillus pumilus 10, which recognizes the asymmetric 5'-cctnagc sequence, has been cloned, sequenced and expressed in escherichia coli . the system comprises four adjacent, similarly oriented genes encoding two m5c mtases and two subunits of bpu 10i enase (34.5 and 34 kda). both bpu10ir genes either in cis or trans are needed for the manifestation of r. bpu 10i activity. subunits of r. bpu 10i, purified to apparent homogeneity, are both required for cleavage activity. ... | 1998 | 9461472 |
| gene cloning, transcriptional analysis, purification, and characterization of phenolic acid decarboxylase from bacillus subtilis. | bacillus subtilis displays a substrate-inducible decarboxylating activity with the following three phenolic acids: ferulic, p-coumaric, and caffeic acids. based on dna sequence homologies between the bacillus pumilus ferulate decarboxylase gene (fdc) (a. zago, g. degrassi, and c. v. bruschi, appl. environ. microbiol. 61:4484-4486, 1995) and the lactobacillus plantarum p-coumarate decarboxylase gene (pdc) (j.-f. cavin, l. barthelmebs, and c. diviès, appl. environ. microbiol. 63:1939-1944, 1997), ... | 1998 | 9546183 |
| expression of a bacillus thuringiensis delta-endotoxin gene by bacillus pumilus. | the delta-endotoxin genes from bacillus thuringiensis were introduced into a rhizosphere-inhabiting bacillus pumilus isolate to create a delta-endotoxin expression and delivery system for subterranean feeding insects such as the larvae of pale western cutworm (agrotis orthogonia morrison (lepidoptera: noctuidae)). preliminary experiments indicated that bacillus thuringiensis subsp. kurstaki cultures were toxic to pale western cutworm larvae. three different cry genes from bacillus thuringiensis ... | 1998 | 9606908 |
| [immune reaction to the heterologic test-antigen in mice with anti-cancer vaccine]. | the stimulated influence of the antitumoral vaccine, prepared with the help of filtrate of the bacillus mesentericus ab-56 or the antibiotic, extracted from it, on the immune answer to heterologic test-antigen (ram erythrocytes) was established. while the vaccine injection to laboratory animals with developing tumor their capacity to resist to the tumors was rising up. | 1997 | 9615014 |
| isolation and partial characterization of antifungal metabolites of bacillus pumilus. | antifungal metabolites produced by bacillus pumilus in potato dextrose broth (pdb) were isolated from culture supernatant fluid by precipitation with ammonium sulphate. the antifungal metabolites inhibited mycelial growth of many species of aspergillus, penicillium and fusarium. they also inhibited production of aflatoxins, cyclopiazonic acid, ochratoxin a and patulin. the metabolites were heat-stable and remained active after sterilization at 121 degrees c for 15 min. their activity was stable ... | 1998 | 9717280 |
| inhibition of aflatoxin production of aspergillus parasiticus nrrl 2999 by bacillus pumilus. | six isolates of bacillus pumilus were tested for their ability to inhibit aflatoxin production of aspergillus parasiticus nrrl 2999 in yeast extract sucrose (yes) broth. aflatoxin production was inhibited in both simultaneous and deferred antagonism assays, suggesting that the inhibitory activity was due to extracellular metabolites(s) produced in cell-free supernatant fluids of cultured broth. the inhibition was not due to organic acids or hydrogen peroxide produced by b. pumilus since the inhi ... | 1997 | 9735015 |
| expression of listeriolysin o and acta by intracellular and extracellular listeria monocytogenes. | listeria monocytogenes requires listeriolysin o (llo) and acta, the products of hly and acta, respectively, to establish a productive intracellular infection. llo is essential for vacuolar lysis and entry into the cytosol, while acta is required for bacterial spread to adjacent cells. we have used a transcriptional reporter gene system to compare the expression of acta and hly during intracellular growth to that during growth in broth cultures. the hly and acta genes were transcriptionally fused ... | 1999 | 9864207 |
| [estimation of probiotics by infection model of infant rabbit with enterohemorrhagic escherichia coli o157:h7]. | we examined the preventative effect against enterohemorrhagic escherichia coli o157:h7 (ehec) infection in rabbits on administration of probiotics contained in streptococcus faecalis, clostridium butyricum and bacillus mesentericus. the probiotics were administered to 5 days old japanese white rabbits throughout the experiment. inoculation of 10(6) colony forming units (cfus) of ehec per rabbit was tried 8 days after birth. three days after ehec inoculation, in the control group (not administere ... | 1998 | 9916417 |
| does probiotics administration decrease serum endotoxin levels in infants? | the aim of this study was to examine whether administration of probiotics to infants can change the ratio of intestinal flora and thereby decrease serum endotoxin produced by potentially pathogenic microorganisms. | 1999 | 10052803 |
| purification and mode of action of an alkali-resistant endo-1, 4-beta-glucanase from bacillus pumilus. | alkaline endo-1,4-beta-d-glucanase was secreted by bacillus pumilus grown in submerged culture on a combination of oat spelt xylan and corn starch as carbon sources. the enzyme was purified to homogeneity by sephacryl s-200 and q-sepharose column chromatography. the protein corresponded to molecular mass and pi values of 67 kda and 3.7, respectively. the enzyme was optimally active at ph 7.0-8.0 and 60 degrees c and retained 50% of its optimum activity at ph 12. the most notable characteristic o ... | 1999 | 10087165 |
| [expression of secreted guanyl-specific ribonuclease genes from bacillus intermedius and bacillus pumilus in bacillus subtilis cells]. | plasmids with whole genes for ribonucleases from b. intermedius (binase) and b. pumilis (rnase bp) assembled with the whole gene of barstar, a specific intracellular inhibitor, are constructed. the resultant plasmids pmz55 and pmz56 effectively express binase and rnase bp genes in b. subtilis cells. a medium for maximum expression of rnase genes by recombinant strains is developed. the expression of binase and rnase bp genes in b. subtilis cells is negatively regulated by exogenic inorganic phos ... | 1999 | 10190104 |
| purification and characterization of an acetyl xylan esterase from bacillus pumilus. | bacillus pumilus ps213 was found to be able to release acetate from acetylated xylan. the enzyme catalyzing this reaction has been purified to homogeneity and characterized. the enzyme was secreted, and its production was induced by corncob powder and xylan. its molecular mass, as determined by gel filtration, is 190 kda, while sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed a single band of 40 kda. the isoelectric point was found to be 4.8, and the enzyme activity was optimal a ... | 1998 | 10215579 |
| the membranotropic activity of cyclic acyldepsipeptides from bacterium bacillus pumilus, associated with the marine sponge ircinia sp. | the isolate of bacillus pumilus associated with the marine sponge ircinia sp. produced the surfactin-like lipopeptides, cyclic acyldepsipeptides. the hemolytic activity of individual cyclic acyldepsipeptides, bacircines (bi) 2, 3, 4, 5 and 5a having different acyl side chain structures (anteiso-c13, iso-c14, normal-c14, anteiso-c15, and iso-c15, respectively) was studied. the hemolytic power of bacircines depended on both the structure of the side chain (n->iso->anteiso-) and ph values (5.6 and ... | 1999 | 10219990 |
| expression of the genes for guanyl-specific ribonucleases from bacillus intermedius and bacillus pumilus is regulated by the two component signal transduction system phop-phor in b. subtilis. | promoters of the genes for guanyl-specific ribonucleases, secreted by b. intermedius (binase) and b. pumilus (rnase bp) in phosphate deficient conditions, contain regions similar to appropriate consensus sequences in promoters of the pho regulated genes of b. subtilis. a number of genes expressed in response to phosphate starvation in b. subtilis are regulated by the two component signal transduction system phop-phor. expression of recombinant genes for binase and rnase bp in b. subtilis strains ... | 1999 | 10220898 |
| purification and characterization of a soybean-milk-coagulating enzyme from bacillus pumilus tyo-67. | bacillus pumilus tyo-67 was isolated from tofu (soybean curd) as the best producer of a soybean-milk-coagulating enzyme, induced by the addition of soybean protein to the growth medium. the enzyme was purified approximately 30-fold with an 11% yield. the homogeneous preparation of the enzyme showed that it is a monomer with a molecular mass of about 30 kda and has an isoelectric point at ph 9.75. the results of amino acid composition analyses showed that the enzyme is rich in alanine, aspartic a ... | 1999 | 10341429 |
| the influence of bacterial inoculants on the microbial ecology of aerobic spoilage of barley silage. | the aerobic decomposition of barley silage treated with two inoculants (laca and lacb) containing mixtures of lactobacillus plantarum and enterococcus faecium was investigated over a 28-day period. initially, yeast and bacterial populations were larger in silage inoculated with laca than in silage treated with lacb or water alone (control). differences in the succession of yeasts in silage treated with laca were observed relative to the other two treatments. from silage treatment with laca, issa ... | 1999 | 10349723 |
| electrospray ionization multiple-stage tandem mass spectrometric analysis of diglycosyldiacylglycerol glycolipids from the bacteria bacillus pumilus. | electrospray ionization (esi) combined with multiple-stage tandem mass spectrometry (ms(n)) was used to directly analyze the glycolipid mixture from bacteria bacillus pumilus without preliminary separation. full scan esi-ms revealed the composition of picomole quantities of glycerolglycolipid species containing c(14)-c(19) fatty acids, some of which were monounsaturated. two main components were identified from their molecular masses and fragmentation pathways. the fragmentation pathway of the k ... | 1999 | 10407296 |
| gamma irradiation for terminal sterilization of 17beta-estradiol loaded poly-(d,l-lactide-co-glycolide) microparticles. | 17beta-estradiol-loaded microparticles using poly-(d, l-lactide-co-glycolide) polymer (plg) were prepared by a modified spray-drying method and the effects of gamma-irradiation on drug substance, polymer and microparticles were investigated. irradiation doses ranging from 5.1 to 26.6 kgy were applied using a 60co-radiation source. 17beta-estradiol drug substance showed excellent stability against gamma-irradiation in the investigated dose range, whereas microencapsulated estradiol seems to be co ... | 1999 | 10469916 |
| [expression of genes for guanyl-specific ribonucleases in bacillus intermedius and bacillus pumilus is regulated by the phop-phor two-component signal transduction system of pho regulon of bacillus subtilis]. | promoters of the genes of guanyl-specific ribonucleases of bacillus intermedius (binase) and bacillus pumilus (rnase bp) were found to contain sequences homologous to those recognizable by the regulatory protein phop in the promoters of the pho regulon of b. subtilis, as well as regions partially homologous to the binding sites of another regulatory protein, phob, in the promoters of the pho regulon of escherichia coli. the role of the two-component regulatory systems phop-phor and phob-phor in ... | 1999 | 10495972 |
| the production of 'kpaye'--a fermented condiment from prosopis africana (guill and perr) taub. seeds. | 'kpaye', a fermented condiment from prosopis africana seeds was produced using the traditional method. bacillus subtilis, bacillus licheniformis and bacillus pumilus were consistently isolated in the fermentations lasting 120 h. 'kpaye'production involved a rise in ph, moisture content and total free amino acids while titratable acidity and reducing sugar decreased gradually after 24 h and 72 h of fermentation respectively. | 1999 | 10574093 |
| the trap-like spla protein is a trans-acting negative regulator of spore photoproduct lyase synthesis during bacillus subtilis sporulation. | uv resistance of bacterial endospores derives from a unique dna photochemistry in which the major uv photoproduct is the thymine dimer 5-thyminyl-5,6-dihydrothymine (spore photoproduct [sp]) instead of cyclobutane pyrimidine dimers. repair of sp during spore germination is due in large part to the activity of the enzyme sp lyase encoded by splb, the second cistron of the splab operon. expression of the splab operon in bacillus subtilis is transcriptionally activated by the esigma(g) form of rna ... | 2000 | 10629212 |
| spatio-temporal distribution of naturally occurring bacillus spp. and other bacteria on the phylloplane of soybean under field conditions. | bacterial populations were surveyed on phylloplane of field-grown soybean every 20 days in southern buenos aires, argentina. the crop was disease-free and no pesticides were applied. out of 523 bacterial isolates (mainly gram positive bacteria) from upper and lower leaves, 134 of them were bacillus spp. bacillus pumilus was the dominant species followed by bacillus subtilis. the proportion of bacillus spp. decreased from 80% of all bacterial isolates in early stages to 0% at harvest, whereas the ... | 1999 | 10629969 |
| a new factor from bacillus mesentericus which promotes the growth of bifidobacterium. | it was reported previously that supernatants of cultures of bacillus mesentericus to-a promote the growth of bifidobacterium species. in this study, a new growth-promoting factor, bm-1, was purified from the supernatant of such a culture and its chemical structure was determined. bm-1 was identified as 3,3-dihydroxyazetidine, and it promoted the growth of several strains of bifidobacterium. | 2000 | 10738983 |
| soybean-milk-coagulating activity of bacillus pumilus derives from a serine proteinase. | a proteolytic enzyme from bacillus pumilus strain tyo-67, which was able to coagulate the protein in soybean milk, was characterized enzymologically. the optimum ph and temperature for its activities were 9.0 and 50 degrees c, respectively. the enzyme was strongly believed to be a serine proteinase because it was completely inhibited by the addition of diisopropyl fluorophosphate or phenylmethanesulfonyl fluoride. hammerstein milk casein, cytochrome c and soybean protein were good substrates for ... | 2000 | 10803893 |
| the acetyl xylan esterase of bacillus pumilus belongs to a family of esterases with broad substrate specificity. | the bacillus pumilus gene encoding acetyl xylan esterase (axe) was identified and characterized. the axe gene was expressed and the recombinant enzyme produced in escherichia coli was purified and characterized. the recombinant enzyme displayed similar properties to the acetyl xylan esterase (axe) purified from b. pumilus. the axe primary structure was 76% identical to the cephalosporin c deacetylase of b. subtilis, and 40% to two recently identified axes from thermoanaerobacterium and thermotog ... | 2000 | 10878123 |
| sequence of the gene encoding an alkaline serine proteinase of bacillus pumilus tyo-67. | the complete nucleotide sequence of the gene encoding an alkaline serine proteinase (aprp) of bacillus pumilus tyo-67 was determined. the sequence analysis showed an open reading frame of 1,149 bp (383 amino acids) that encoded a signal peptide consisting of 29 residues and a propeptide of 79 residues. the deduced 3 amino acid residues, d32, h64, and s221, were identical with 3 essential amino acids in the catalytic center of subtilases. the sequence around these residues revealed that aprp was ... | 2000 | 10888358 |
| extraction in aqueous two-phase systems of alkaline xylanase produced by bacillus pumilus and its application in kraft pulp bleaching. | the aim of this work was to extract and to purify xylanase, produced by bacillus pumilus from the crude fermentation broth, using aqueous two-phase systems (atps). the xylanase was extracted by partitioning in atps composed of phosphate and polyethylene glycol (peg). the effect of tie-line length, peg molecular mass and nacl concentrations upon the purification factors and yields of xylanase were investigated by statistical design. the best system studied was that containing 22% peg6000, 10% k2h ... | 2000 | 10942305 |
| antibacterial activity of water and acetone extracts of the roots of euclea natalensis. | water and acetone extracts of the roots of euclea natalensis a.dc. were investigated for their in vitro antibacterial properties. the gram-positive bacteria tested appeared to be more susceptible to the extracts than the gram-negative bacteria. the water and acetone extracts inhibited the growth of bacillus cereus, bacillus pumilus, bacillus subtilis, micrococcus kristinae and staphylococcus aureus at concentrations ranging between 0.1 and 6.0 mg/ml. the water extract did not exert any inhibitor ... | 2000 | 10967488 |
| coexpression of the bacillus pumilus beta-xylosidase (xynb) gene with the trichoderma reesei beta xylanase 2 (xyn2) gene in the yeast saccharomyces cerevisiae. | the xynb gene encoding the bacillus pumilus beta-xylosidase was expressed separately and jointly with the trichoderma reesei beta-xylanase (xyn2) gene in the yeast saccharomyces cerevisiae. both genes were placed under the transcriptional control of the glucose-derepressible alcohol dehydrogenase 2 promoter (adh2p) and terminator (adh2t) sequences. the xynb gene was fused in frame to the yeast mating factor alpha1 secretion sequence (mfalpha1s) to effect secretion in s. cerevisiae. the fusion pr ... | 2000 | 10968632 |
| [studies on submerged fermentation of alkaline beta-1,4-glycanases by bacillus pumilus a-30]. | the effects of stirring rate, ph control and feed time of (nh4)2so4 on beta-1,4-glycanases were investigated. the operating conditions of batch fermentation and fed-batch culture were optimized. the results showed there were a large amount of bacterial cells adsorbing on the surface of wheat bran, and stirring rate had a pronounced influence on the adsorption and the production of beta-1,4-glycanases. the uncontrolled ph could promote the induction of beta-1,4-glycanases. when (nh4)2so4 was fed ... | 2000 | 11051825 |
| the dppa gene of bacillus subtilis encodes a new d-aminopeptidase. | different strains of bacillus were screened for their ability to hydrolyse d-alanyl-p-nitroanilide. activity was detected in bacillus pumilus, bacillus brevis, bacillus licheniformis 749i and bacillus subtilis 168. the last strain was the best producer and was selected for the production and purification of the enzyme. the determination of the n-terminal sequence identified the enzyme as the product of the dppa gene (previously named dciaa) belonging to the dipeptide abc transport (dpp) operon e ... | 2000 | 11069674 |
| a rapid procedure for isolating chromosomal dna from lactobacillus species and other gram-positive bacteria. | this study describes a rapid procedure for the isolation of genomic dna from various gram-positive bacteria. species tested included lactobacillus delbrueckii subsp. lactis atcc 4797, lact. acidophilus n2, staphylococcus aureus, staph. epidermidis, propionibacterium jensenii p126, bacillus pumilus and enterococcus faecalis. our technique for chromosomal dna isolation circumvents the need for phenol-chloroform extractions and caesium chloride gradients. isolated dna is consistently greater than 2 ... | 2001 | 11169042 |
| [fermentation conditions of engineering strain utilizing starch for production of alkaline proteinase]. | the highest activity of alkaline proteinase produced by bacillus pumilus c172(pbx 96) transformant was 9,000 u/ml in shaking flask where corn meal and bean cake meal were carbon and nitrogen sources, respectively. the enzyme activity was raised under the conditions of initial ph 7.0, mgcl2 instead of mgso4, and glucose (0.1%) in the substrate. the fermentation of c172(pbx 96) transformant was carried out with parameters of ph, reducing sugar, total sugar and enzyme activity. | 1997 | 11189381 |