Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| biosynthesis of linkage units for teichoic acids in gram-positive bacteria: distribution of related enzymes and their specificities for udp-sugars and lipid-linked intermediates. | the distribution and substrate specificities of enzymes involved in the formation of linkage units which contain n-acetylglucosamine (glcnac) and n-acetylmannosamine (mannac) or glucose and join teichoic acid chains to peptidoglycan were studied among membrane systems obtained from the following two groups of gram-positive bacteria: group a, including bacillus subtilis, bacillus licheniformis, bacillus pumilus, staphylococcus aureus, and lactobacillus plantarum; group b, bacillus coagulans. all ... | 1989 | 2914877 |
| thermoinducible transcription system for bacillus subtilis that utilizes control elements from temperate phage phi 105. | we describe a thermoinducible-expression system for bacillus subtilis which utilized an early promoter-operator sequence from temperate phage phi 105 and the thermolabile prophage repressor from the phage variant phi 105 cts23. the system operated at the transcriptional level to control expression in b. subtilis of the cat-86 gene derived from bacillus pumilis. details of the strategies used to isolate the early phage promoter are described. this promoter lay in close proximity to the prophage r ... | 1985 | 2993234 |
| nucleotide sequence analysis of the gene specifying the bifunctional 6'-aminoglycoside acetyltransferase 2"-aminoglycoside phosphotransferase enzyme in streptococcus faecalis and identification and cloning of gene regions specifying the two activities. | the gene specifying the bifunctional 6'-aminoglycoside acetyltransferase [aac(6')] 2"-aminoglycoside phosphotransferase [aph(2")] enzyme from the streptococcus faecalis plasmid pip800 was cloned in escherichia coli. a single protein with an apparent molecular weight of 56,000 was specified by this cloned determinant as detected in minicell experiments. nucleotide sequence analysis revealed the presence of an open reading frame capable of specifying a protein of 479 amino acids and with a molecul ... | 1986 | 3015884 |
| [effect of inhibitors and cations on the proteolytic activity of bacillus mesentericus]. | the effect of some inhibitors and bivalent metal cations (mn2+, ca2+, fe2+, zn2+, mg2+, co2+ and cu2+) on the proteolytic activity of two bacillus mesentericus strains (strain 8 and strain 64 m-variant) was comparatively studied. the both enzymes were shown to be serine proteinases, but the proteinase of strain 64 was also a metal-dependent enzyme. metal ions exerted no essential effect on the proteinase of strain 8. ca2+ and mg2+ ions stimulated the proteinase activity of strain 64 whereas fe2+ ... | 1988 | 3054437 |
| [use of starch-containing media for cultivation of bacillus mesentericus]. | when cultivating bacillus mesentericus to produce proteinases it is advisable to use more available and cheap carbon sources--native maize meal or potato starch--instead of maltose; the products of their complete hydrolysis inhibit the biosynthesis of enzymes. | 1988 | 3074302 |
| sterilization of therapeutic immunoadsorbents by ionizing radiation. | the application of ionizing radiation (gamma-rays from 60co) to sterilize therapeutic immunoadsorbents (ia)s was investigated. the ias were porous bead carriers immobilizing anti-ige antibodies and were irradiated in both the freeze-dried and precipitated (wet) states. the ige removal (%), the ige adsorption capacity of ia, was acceptable in terms of practical use even after an irradiation dose of 2.5 mrad in the precipitated state; the anti-ige antibody itself lost much of its activity, possibl ... | 1986 | 3084388 |
| regulatory elements common to the bacillus pumilus and bacillus subtilis trp operons. | the trp operon regulatory region of bacillus pumilus was cloned and sequenced. the cloned b. pumilus trp promoter-leader region functioned in bacillus subtilis to express the adjacent leukocyte interferon a gene on a multicopy transcriptional fusion plasmid, pbpifi. in strains carrying this plasmid, anthranilate synthetase levels were elevated, possible due to titration of a b. subtilis trp regulatory factor by multiple copies of the transcript of the plasmid-borne b. pumilus trp leader region. ... | 1986 | 3091579 |
| numerical taxonomy of bacillus isolated from orally administered drugs. | numerical taxonomy procedures were used to study 118 strains of bacillus isolated from non-sterile drugs prepared for oral administration. similarities between pairs of strains were calculated by the simple matching coefficient of sokal and michener (ssm). each strain was tested for 60 unit characters and three clusters were defined. the strains in each cluster presented a similarity level of at least 60%. cluster a comprised the strains identified as bacillus cereus (ssm = 93.13%), cluster b co ... | 1986 | 3096933 |
| [integration of the plasmid carrying the genes for tryptophan metabolism of bacillus mesentericus into the chromosome of bacillus subtilis]. | integration of several tryptophan operon genes from bacillus mesentericus into the chromosome of bac. subtilis was carried out. the genetic material exchange between these two species is usually impossible in the process of transformation, because of poor homology. the integration only took place after the genes of bac. mesentericus were introduced in bac. subtilis cells on a hybrid plasmid. plasmid markers also integrated into the chromosome, together with heterologous dna. the integration occu ... | 1987 | 3104137 |
| [structural-functional characteristics of bacillus mesentericus promoters cloned in bacillus subtilis cells]. | bacillus mesentericus dna fragments carrying promoters were cloned in bacillus subtilis. the nucleotide sequences of four promoters, the sites of transcription initiation and the levels of promoter-mediated expression of ppl603 cat gene at different stages of cell growth were determined. it was identified, that the two promoters (p435 and p442) are the typical sigma 43-rna polymerase promoters. promoter p462 possessed the unusual nucleotide sequence and induced the expression of indicator cat ge ... | 1987 | 3110606 |
| catalytic versatility of bacillus pumilus beta-xylosidase: glycosyl transfer and hydrolysis promoted with alpha- and beta-d-xylosyl fluoride. | bacillus pumilus beta-xylosidase, an enzyme considered restricted to hydrolyzing a narrow range of beta-d-xylosidic substrates with inversion of configuration, was found to catalyze different stereochemical, essentially irreversible, glycosylation reactions with alpha- and beta-d-xylopyranosyl fluoride. the enzyme promoted the hydrolysis of beta-d-xylopyranosyl fluoride at a high rate, v = 6.25 mumol min-1 mg-1 at 0 degrees c, in a reaction that obeyed michaelis-menten kinetics. in contrast, its ... | 1987 | 3111528 |
| [cloning in cells of bacillus subtilis and study of the characteristics of promoter regions of dna of bacillus mesentericus and phage spo2]. | 11 fragments of bacillus mesentericus and phage spo1 dna containing promoters were cloned in bacillus subtilis. nucleotide sequences of these fragments were determined and s1 mapping of transcriptional start points was performed. it was found that some fragments contained tandem or overlapping promoters. nucleotide sequences of bacillus mesentericus promoters were compared with known promoters from bacilli. promoter activities were determined in different phases of cell growth. nucleotide sequen ... | 1988 | 3128731 |
| chloramphenicol acetyltransferase specified by cat-86: relationship between the gene and the protein. | gene cat-86 is chloramphenicol (cm)-inducible and specifies cm acetyltransferase, cat-86. the gene was previously cloned from the dna of a strain of bacillus pumilus. in the present study we report the construction of a constitutively expressed version of cat-86 that permits high-level expression of the gene on a plasmid in b. subtilis. a method is described that allows very rapid purification of cat-86 protein to homogeneity. the sequence of 13 n-terminal amino acids of purified cat-86, as well ... | 1988 | 3149605 |
| resistance of some common fungi to gamma irradiation. | ten species of fungi representing the genera alternaria, aspergillus, caldosporium, curvularia, fusarium, and penicillium were examined for their relative resistance to gamma irradiation from a 137cs source. inactivation doses for dematiaceous fungi in agar medium ranged from 0.6 to greater than 1.7 megarads, whereas those for moniliaceous fungi were less than 0.3 megarad. d10 values (the dose required to reduce the inoculum by 1 log) for curvularia geniculata (greater than 0.29 megarad) exceede ... | 1988 | 3178216 |
| [characteristics of the extracellular fluorescing pigment of bacillus mesentericus strain 90 r-variant]. | 1988 | 3247862 | |
| [effect of environmental factors on lectin biosynthesis by bacillus mesentericus]. | 1988 | 3249569 | |
| the influence of gamma-irradiation upon the chemical and biological properties of insulin. | partially purified insulin preparations of bovine and porcine origin, were subjected to gamma-irradiation with doses ranging from 1.0 up to 25 kgy (0.1-2.5 mrad) at 0 degrees c or ambient temperature. the susceptibility of insulin to the irradiation was determined by chromatography, electrophoresis and assay of the biological activity. the sterilizing effect of the gamma-irradiation was investigated for bacillus pumilus as well as for artificial mixtures of lactose and several bacilli. it is con ... | 1987 | 3302930 |
| [fibrinolytic activity of natural variants of bacillus mesentericus]. | the natural variability of the ability to synthesize proteinases by bacillus mesentericus 64 was studied. the population of this strain was shown to be heterogeneous. three types of variants (s, m and p) differed in the morphology of their colonies and in the culture characteristics from the typical colonies of the parent strain. the caseinolytic activity of the m variant was three times as high as that of the parent strain, and it also had an elevated fibrinolytic activity and a high rate of bl ... | 1987 | 3329699 |
| bacillus infections in patients with cancer. | eighteen febrile patients experienced 24 episodes of bacillus bacteremias from january 1978 to june 1986. bacillus species isolated included bacillus cereus (eight cases), bacillus circulans (three), bacillus subtilis (two), bacillus pumilus (two), bacillus licheniformis (one), bacillus sphaericus (one), bacillus coagulans (one), and six that could not be speciated. fifteen patients had lymphoma or leukemia and three had breast cancer. nine patients were neutropenic (polymorphonuclear neutrophil ... | 1988 | 3401098 |
| the effects of deletions in the leader sequence of cat-86, a chloramphenicol-resistance gene isolated from bacillus pumilus. | the cat-86 gene of bacillus pumilus, specifying a cm-inducible cat enzyme, was cloned previously into b. subtilis on plasmid pub110. various lines of evidence suggest that control of expression of this gene is at the level of translation and involves inverted complementary repeat sequences 5' to the initiation codon. a series of deletions have been generated in this region and their effects on the induction of cat-86 observed in b. subtilis, escherichia coli and a number of ribosomal mutant stra ... | 1987 | 3477516 |
| effects of oxygen free radical scavengers on the membrane myoinositol dehydrogenase of bacillus pumilus strain 5. | micromolar amounts of superoxide dismutase (sod) or parabenzoquinone (pbq) inhibit the membrane-bound myoinositol dehydrogenase of bacillus pumilus strain 5 in the mode of this enzyme transferring electrons to 2,6-dichlorophenol indophenol (dcpip). the inhibition trends are similar to those reported earlier by us for the inhibition by mannitol and benzoate. we postulate that the transfer of electrons from the enzyme to dcpip involves in its rate-limiting step, a catalytic intermediate in the nat ... | 1987 | 3508132 |
| [fibrinolytic activity of bacillus mesentericus strains]. | two bacillus mesentericus strains with a high activity of proteolytic enzymes having the thrombolytic action were selected from a group of its collection strains. the effect of different carbon sources on the synthesis of proteases was studied. a growth medium containing potato broth (10%), peptone (0.5%) and lactose (0.5%) allowed one to obtain a cultural broth dissolving human blood clots within 2.5 to 3 hours in experiments in vitro. | 1986 | 3523167 |
| isolation of an aminoglycoside hypersensitive mutant and its application in screening. | an aminoglycoside hypersensitive mutant, kp-126, was isolated from the aminoglycoside-resistant strain, kp-8, of klebsiella pneumoniae through selection using sorbistin, a non-aminocyclitol-aminoglycoside antibiotic. the mutant kp-126 was approximately 100-fold more sensitive to sorbistin than the parent strain kp-8. the mutant also showed hypersensitivity to various aminocyclitol-aminoglycoside antibiotics. k. pneumoniae kp-126 was used in screening and a new aminoglycoside antibiotic, 3,3'-neo ... | 1986 | 3531132 |
| crystallization and preliminary x-ray studies of bacillus pumilus ipo xylanase. | the xylan-degrading enzyme xylanase, from bacillus pumilus ipo, has been crystallized. the crystals are monoclinic, space group p21 with a = 40.8 a, b = 66.8 a, c = 34.7 a and beta = 103.0 degrees. the asymmetric unit contains one molecule of mr 22,500. the crystals diffract to at least 2.5 a resolution, and they are suitable for x-ray crystal structure analysis at high resolution. | 1987 | 3586024 |
| comparative studies of lipoteichoic acids from several bacillus strains. | structural studies were carried out on lipoteichoic acids obtained from defatted cells of 10 bacillus strains by phenol-water partition followed by chromatography on deae-sephacel and octyl-sepharose columns. a group of the tested bacteria (group a), bacillus subtilis, bacillus licheniformis, and bacillus pumilus, was shown to have a diacyl form of lipoteichoic acids which contained d-alanine, d-glucose, d-glucosamine, fatty acids, and glycerol in molar ratios to phosphorus of 0.35 to 0.69, 0.07 ... | 1986 | 3733670 |
| [interaction of the glycoprotein from the bacillus pumilis cell wall with liposomes]. | the methods of centrifugation and gel-filtration on sephadexes g-50 and g-150 were used to study the interaction of bacillus pumilis cell wall glycoprotein component having the molecular weight of 50 kda (gp-50) with lyposomes from bacterial lipids. gp-50 is shown to sorb on such liposomes and disturb their barrier properties inducing yield of low-molecular label. gp-50 exerts no effect on properties of liposomes from egg lecithin. electrostatic forces are supposed to play a decisive role in ini ... | 1987 | 3810893 |
| the use of synthetic oligonucleotides with universal templates for rapid dna sequencing: results with staphylococcal replicon pc221. | a rapid sequencing strategy has been devised and applied to determine the complete nucleotide sequence (4555 bp) of staphylococcus aureus plasmid pc221. the entire replicon was cloned into phage m13mp8 in both orientations to provide 'universal templates' for primed dna synthesis from internally-sited oligonucleotide primers. the latter were synthesized by a modification of a recently described paper disc method which employs phosphotriester chemistry. less than 4 weeks was required for the synt ... | 1985 | 3860383 |
| the contribution of endogenous and exogenous effects to radiation-induced damage in the bacterial spore. | radical scavengers such as polyethylene glycol 400 and 4000 and bovine albumin have been used to define the contribution of exogenous and endogenous effects to the gamma-radiation-induced damage in aqueous buffered suspensions of bacillus pumilus spores. the results indicate that this damage in the bacterial spore is predominantly endogenous both in the presence of 1 atmosphere of oxygen, and in anoxia. | 1985 | 3873432 |
| risk of group a meningococcal disease: bacterial interference and cross-reactive bacteria among mucosal flora. | during outbreaks of group a meningococcal disease in seattle, wash., and portland, oreg., we studied the mucosal flora of the affected population and comparison groups to identify possible determinants of susceptibility and resistance to disease. antimeningococcal immunoglobulin a can block the bactericidal activity of specific antibodies of other classes and has been associated with susceptibility in adults. we used immunoprecipitation and fluorescent-antibody techniques to detect mucosal micro ... | 1985 | 3928679 |
| isolation of thermophilic mutants of bacillus subtilis and bacillus pumilus and transformation of the thermophilic trait to mesophilic strains. | thermophilic mutants were isolated from mesophilic bacillus subtilis and bacillus pumilus by plating large numbers of cells and incubating them for several days at a temperature about 10 degrees c above the upper growth temperature limit for the parent mesophiles. under these conditions we found thermophilic mutant strains that were able to grow at temperatures between 50 degrees c and 70 degrees c at a frequency of less than 10(-10). the persistence of auxotrophic and antibiotic resistance mark ... | 1985 | 3934332 |
| 1h-2-benzopyran-1-one derivatives, microbial products with pharmacological activity. conversion into orally active derivatives with antiinflammatory and antiulcer activities. | a novel gastroprotective substance, 6-[[1(s)-[3(s),4-dihydro-8-hydroxy-1-oxo-1h-2-benzopyran-3-yl] -3-methylbutyl]amino]-4(s),5(s)-dihydroxy-6-oxo-3(s)-ammoniohexanoate (ai-77-b, 1), isolated from a culture broth of bacillus pumilus ai-77, was chemically modified to prodrugs that are active by oral dosing. compound 1 was lactonized and then monoalkylated at the primary amine position. six n-alkylated gamma-lactone derivatives of 1 (with alkyl chains being methyl 5a, ethyl 5b, n-propyl 5c, n-buty ... | 1985 | 3965711 |
| structural studies on the linkage unit between poly(n-acetylglucosamine 1-phosphate) and peptidoglycan in cell walls of bacillus pumilus ahu 1650. | structural studies were carried out on the polymer chains and their linkage regions in two kinds of teichoic acids, poly(n-acetylglucosamine 1-phosphate) [poly(glcnac-1-p)] and glycerol teichoic acid, bound to peptidoglycan in the cell walls of bacillus pumilus ahu 1650. the poly(glcnac-1-p)-glycan complex isolated from lysozyme digests of the cell walls contained mannosamine and glycerol as minor components. on the basis of proton nmr spectroscopic data and isolation of n-acetylglucosamine 4-ph ... | 1985 | 3996410 |
| selection and characterization of a pleomorphic form and an l form of bacillus pumilus nrrl b-3275. | 1971 | 4106387 | |
| pbpi: a flagella specific bacteriophage mediating transduction in bacillus pumilus. | 1972 | 4111056 | |
| polysaccharides of the genus bacillus cross-reactive with the capsular polysaccharides of diplococcus pneumoniae type 3, haemophilus influenzae type b, and neisseria meningitidis group a. | we studied 174 strains of the genus bacillus for cross-reacting antigens to the capsular polysaccharides of groups a and c meningococcus, types i and iii pneumococcus, and haemophilus influenzae type b. cross-reactions were detected by immunodiffusion in agarose gel by using type-specific antisera and confirmed by absorption and inhibition experiments. of 20 bacillus pumilis strains, six had an antigen cross-reacting with group a meningococcal polysaccharide. other cross-reactions included one s ... | 1973 | 4150383 |
| differentiation within the bacterial flagellum and isolation of the proximal hook. | purified and crude flagellar isolates from cells of bacillus pumilus nrs 236 were treated with acid, alcohol, acid-alcohol, or heat, and were examined electron microscopically in negatively stained and shadow-cast preparations. under certain conditions, each of these agents causes the flagella to break between the proximal hooks and the spiral filaments. in such preparations, filaments are seen in various stages of disintegration, whereas hooks of fairly constant length retain their integrity an ... | 1970 | 4189228 |
| kinetics of initiation of germination of bacillus pumilus spores by hydrostatic pressure. | the kinetics of initiation of germination and inactivation by hydrostatic pressure of phosphate-buffered bacillus pumilus spores is shown to be a consecutive first-order process at 25 c. the effect of increasing pressure at constant temperature was studied, and rate constants were derived by using the criteria of heat resistance, refractility, and stainability. the calculated volume change of activation (deltavdouble dagger) was -139 +/- 6 cm(3)/mole for loss of heat resistance, -158 +/- 8 cm(3) ... | 1970 | 4192983 |
| serine neutral proteinase from bacillus pumilus as metalloenzyme. | 1974 | 4208330 | |
| temperature-induced changes in the sporicidal activity and chemical properties of glutaraldehyde. | freshly prepared 2% acid and alkaline glutaraldehyde solutions were stored at 4, 20, and 37 c. at intervals, samples were removed and changes in ph, ultraviolet spectrum, and sporicidal activity (against bacillus pumilus spores) were recorded. alkaline solutions stored at 4 c showed little changes in these properties, whereas such solutions stored at 37 c became turbid and showed a decrease in ph, marked changes in ultraviolet spectrum, and an almost complete loss of sporicidal activity. interme ... | 1974 | 4213869 |
| [phosphatase activity of intact cells of bacillus subtilis and bacillus mesentericus]. | 1968 | 4308699 | |
| spontaneous auxotrophic and pigmented mutants occurring at high frequency in bacillus pumilus nrrl b-3275. | broth cultures of bacillus pumilus nrrl b-3275 (bpb1) grown at 25, 30, or 37 c contain 1 to 2% spontaneous auxotrophic mutants in both the exponential and stationary phases of growth. of 70 such mutants isolated from cultures grown at 37 c, approximately two-thirds reverted at such a high frequency as to preclude their study. of the remaining 22 mutants, 18 required a single amino acid, 1 required adenine, and 1 required uracil. two of the auxotrophs each required two unrelated amino acids resul ... | 1972 | 4343826 |
| homologous and hybrid complexes of anthranilate synthase from bacillus species. | the subunits of anthranilate synthase were separated and partially purified by sephadex g-100 gel filtration from the following six species of bacillus: bacillus subtilis, bacillus licheniformis, bacillus alvei, bacillus coagulans, bacillus pumilus, and bacillus mascerans. our data suggest that the enzyme from b. alvei is unique among these species. first, the anthranilate synthase complexes are readily dissociated during gel filtration in the absence of glutamine into a large component (aminotr ... | 1974 | 4407252 |
| biochemical studies of two bacillus pumilus plasmids. | bacillus pumilus nrs 576 harbored an estimated two copies per chromosome of a covalently closed, circular (ccc) deoxyribonucleic acid (dna) molecule, the 576 plasmid. the 576 plasmid has a buoyant density of 1.698 g/cm(3) and a molecular weight of about 28 x 10(6). plasmid copy number remained about the same in both exponentially growing and stationary-phase cells. spontaneous variants of nrs 576 that formed spores at an elevated frequency were designated as w mutants. w mutants appeared to have ... | 1974 | 4420778 |
| temperate bacteriophage infectious for asporogenic variants of bacillus pumilus. | bacillus pumilus strain nrrl b-3275 is lysogenic for an inducible, nondefective temperate bacteriophage phi75. phi75 infects and lysogenizes several asporogenic mutants of b. pumilus strain nrs 576 but does not productively infect the spore(+) parent. phi75 dna is a linear duplex with a mol wt of about 29 x 10(6) and a buoyant density of 1.701 g/cm(3). the location of the phi75 prophage attachment site on the chromosome of both host strains is adjacent to a lysine marker. the apparent order is p ... | 1974 | 4431081 |
| [catabolite repression of l-asparaginase biosynthesis in bacillus mesentericus]. | 1974 | 4444557 | |
| [effect of culture homogeneity and the age and quantity of the inoculum on the biosynthesis of amylolytic enzymes by bacillus mesentericus pb]. | 1974 | 4453538 | |
| [isolation and identification of vitamin k2 synthesized by a culture of bacillus mesentericus]. | 1974 | 4463354 | |
| [effect of the chemical structure of polymers on the activity of certain enzymes synthesized by cultures of aspergillus oryzae and bacillus mesentericus]. | 1973 | 4600723 | |
| [effect of n-methyl-n'-nitro-n-nitrosoguanidine on variability of bacillus mesentericus--producer of proteolytic enzymes]. | 1974 | 4620110 | |
| intergenotic transformation of the bacillus subtilis genospecies. | a multiple auxotrophic derivative of bacillus subtilis 168 (strain br151 carrying lys-3, trpc2, metb10) was transformed with deoxyribonucleic acid (dna) isolated from b. subtilis 168, bacillus amyloliquefaciens h, b. subtilis hsr, bacillus pumilus, and bacillus licheniformis. transformation with heterologous dna occurred at a very low frequency for the three auxotrophic markers. heterologous transformation to rifampin resistance was 100 to 1,000 times more efficient than transformation to protot ... | 1972 | 4626541 |
| plasmid in bacillus pumilus and the enhanced sporulation of plasmid-negative variants. | approximately 3% of the deoxyribonucleic acid (dna) of bacillus pumilus nrs576 can be isolated as covalently closed, circular duplex molecules of homogeneous size. the mol wt of the 576 plasmid is approximately 30 million. nrs576 (plasmid(+)) is oligosporogenic; less than 1% of the cells form spores during incubation in liquid ak sporulation medium. variants that form spores at a high frequency (greater than 50% spores in 24 h) occur spontaneously at a frequency of approximately 10(-5). more tha ... | 1973 | 4717517 |
| cryptic plasmid in bacillus pumilus atcc 7065. | 1973 | 4741574 | |
| enzymatic defects in three genetic classes of serine-requiring mutants of bacillus pumilus. | serine-requiring mutants of bacillus pumilus nrrl b-3275 have been divided into three groups based on the position of the mutant loci on the linkage map of this organism. representatives of each group were found deficient in enzymatic activities that constitute the phosphorylated pathway for serine biosynthesis. the evidence suggests that the genes coding for the enzymes of the phosphorylated pathway of serine biosynthesis are not clustered in b. pumilus. | 1973 | 4745428 |
| conformational studies on alkaline protease from bacillus mesentericus. | 1973 | 4755422 | |
| [variability of bacillus mesentericus under the influence of p-nitroaniline]. | 1973 | 4789626 | |
| [variability of bacillus mesentericus]. | 1973 | 4792255 | |
| some properties of the pbp1 transduction system in bacillus pumilus. | bacteriophage pbp1 is a flagella-specific virus that performs generalized transduction in strains of bacillus pumilus. pbp1 is morphologically and serologically distinct from two other flagella-specific phages, pbs1 and sp-15, which perform generalized transduction in certain bacillus species. the dna extracted from pbp1 particles has a buoyant density of 1.690 g/cm(3) in cesium chloride gradients, a melting temperature of 86.1 c, and a sedimentation velocity of 47s in neutral sucrose gradients. ... | 1974 | 4811009 |
| [optimization of the composition of the medium for cultivation of bacillus mesentericus--a producer of amylolytic enzymes]. | 1974 | 4828743 | |
| increased radiation resistance of vegetative bacillus pumilus. | a 4.5-fold increase in vegetative cell radiation resistance of bacillus pumilus e601, the internationally recognized biological standard for irradiation sterilization, was obtained by the repeated passage of resistant survivors through successive sublethal doses of (60)co irradiation. this increase in resistance was accompanied by a corresponding increase in spore resistance through the seventh irradiation passage. by the fifteenth passage, the ability for spore formation was lost. other effects ... | 1974 | 4844266 |
| effects of continuous and interrupted radiation on microorganisms. | various bacterial spores exhibited a wide range of radiation resistance to doses of 0.25 to 2.5 mrad from a cobalt-60 radiation facility. bacillus pumilus and clos-tridium tetani were shown to have the highest degree of resistance when compared with other bacterial sporeformers. b. subtilis e163 was the least resistant of the bacterial spores studied. dried spores contained on cellulose discs were more readily destroyed by gamma-rays than were wet spores under similar conditions. mycobacterium t ... | 1967 | 4963440 |
| [aldolase activity of bacillus subtilis and bacillus mesentericus strains]. | 1969 | 4981327 | |
| identification of bacillus subtilis nrrl b-3275 as a strain of bacillus pumilus. | the physiological and biochemical properties of a species of bacillus previously identified as b. subtilis nrrl b-3275 (b-3275) were compared with those of seven strains of b. pumilus and five strains of b. subtilis. the biotin requirement of b-3275, its inability to hydrolyze starch, and its failure to reduce nitrate indicate that the organism is more closely related to the b. pumilus strains than to those of b. subtilis. hybridization of deoxyribonucleic acid (dna) from b-3275 with that of the ... | 1969 | 4982197 |
| trichinella spiralis: establishment in gnotobiotic mice affected by bacillus mesentericus, b. subtilis, and pseudomonas aeruginosa. | 1969 | 4983055 | |
| purification and some properties of the extracellular protease of bacillus pumilus. | 1970 | 4990355 | |
| [activity and properties of l-asparaginase from bacillus mesentericus 43a]. | 1972 | 5044884 | |
| [antibiotic properties of bacillus mesentericus]. | 1971 | 5092259 | |
| purification and properties of an polygalacturonic acid trans-eliminase produced by bacillus pumilus. | a strain of bacillus pumilus produced an extracellular pectic enzyme with polygalacturonic acid as the substrate. this enzyme, with optimal activity at ph 8.0 to 8.5, produced reaction products that strongly absorbed light at 232 nm, indicating the presence of a pectic acid trans-eliminase (pate). neither pectin esterase nor polygalacturonase was detected in the cell-free culture fluid. chromatographic examination of the end products revealed the presence of large quantities of unsaturated oligo ... | 1971 | 5122802 |
| [repression of protease synthesis in bacillus mesentericus]. | 1969 | 5361505 | |
| genetic analysis in bacillus pumilus by pbsi-mediated transduction. | bacteriophage pbs1 mediates generalized transduction in bacillus pumilus nrrl b-3275 (bpb1). transduction frequencies for single auxotrophic markers are of the order of 10(-4) transductants per plaque-forming unit in crude phage lysates. the characteristics of pbs1 propagated on bpb1 and the properties of the system of transduction are similar to those reported for pbs1 propagated on bacillus subtilis. by transduction, eight amino acid auxotrophic markers in bpb1 have been oriented into two link ... | 1970 | 5413829 |
| synthesis of the extracellular protease by bacillus pumilus. | 1970 | 5469696 | |
| [optimization of the growth conditions for bacillus mesentericus strain 11-11 using a mathematical model of the process in the form of a power function]. | 1970 | 5513411 | |
| linkage groups in bacillus pumilus determined by bacteriophage pbs1-mediated transduction. | two linkage groups were established in bacillus pumilus nrrl b-3275 by bacteriophage pbs1 transduction. group l contains the auxotrophic markers gly, his, and met. group m contains the markers argo, met, ura, and cys. | 1971 | 5573737 |
| [studies on the effect of some nitrogen and carbon sources on the biosynthesis of proteolytic complex of bacillus mesentericus]. | 1967 | 5601519 | |
| [biological spectrum of action of the proteolytic enzyme complex in bacillus mesentericus during its cultivation on synthetic culture media]. | 1967 | 5601520 | |
| cyanide resistance and cyanide utilization by a strain of bacillus pumilus. | 1969 | 5765181 | |
| initiation of germination and inactivation of bacillus pumilus spores by hydrostatic pressure. | the effect of hydrostatic pressures as high as 1,700 atm at 25 c on the heat and radiation resistance of bacillus pumilus spores was studied. phosphate-buffered spores were more sensitive to compression than spores suspended in distilled water. measurements of the turbidity of suspensions, the viability, refractility, stainability, dry weight, and respiratory activity of spores, and calcium and dipicolinic acid release were made for different pressures and times. initiation of germination occurr ... | 1969 | 5773022 |
| partial purification and properties of an induced beta-d-xylosidase of bacillus pumilus 12. | 1969 | 5791586 | |
| [cytotoxic changes in the nucleic acid content in a culture of tumor and normal cells as affected by a complex preparation from bacillus mesentericus ab-56]. | 1981 | 6171711 | |
| 60co-irradiation as an alternate method for sterilization of penicillin g, neomycin, novobiocin, and dihydrostreptomycin. | the effects of the use of 60co-irradiation to sterilize antibiotics were evaluated. the antibiotic powders were only occasionally contaminated with microorganisms. the d-values of the products and environmental isolates were 0.028, 0.027, 0.015, 0.046, 0.15, 0.018, and 0.19 mrads for aspergillus species (uc 7297, 7298), a. fumigatus (uc 7299), rhodotorula species (uc 7300), penicillium oxalicum (uc 7269), pseudomonas maltophilia (uc 6855), and a biological indicator microorganism, bacillus pumil ... | 1983 | 6186803 |
| specific in vitro binding of a plasmid to a membrane fraction of bacillus subtilis. | a model system has been developed to study the in vitro binding of a plasmid to the membrane fraction from bacillus subtilis. the plasmid dna molecule used in these studies was psl103 (8.0 kilobases), a chimeric plasmid consisting of a staphylococcus aureus plasmid (pub110, 4.5 kilobases) and a dna fragment (3.5 kilobases) from bacillus pumilus carrying trpc+ gene. this plasmid replicates in b. subtilis cells, and its in vivo membrane binding (as well as its replication) is dependent on the prod ... | 1983 | 6300855 |
| nucleotide sequence of a bacillus pumilus gene specifying chloramphenicol acetyltransferase. | gene cat-86 of bacillus pumilus, specifying chloramphenicol-inducible chloramphenicol acetyltransferase, was previously cloned in bacillus subtilis on plasmid pub110. the nucleotide sequence of cat-86 indicates that the gene encodes a protein of 220 amino acids and contains ttg as the translations-initiation codon. the proteins specified by cat-86 and the cat genes present on pc194, pc221 and tn9 appear to share regions of amino acid sequence similarity. cat-86 is a structural gene on the b. sub ... | 1983 | 6315534 |
| a novel glycerophosphodiesterase from bacillus pumilus. | a novel glycerophosphodiesterase activity was detected in extracts from phosphate-starved bacillus pumilus dsm27 cells. the enzyme had a substrate specificity for glycerophosphodiester bonds and the reaction product formed with partially purified enzyme was (sn)-glycero-3-phosphate. purified cell wall teichoic acid of the polyglycerophosphate type, as well as deacylated, unsubstituted lipoteichoic acid of the polyglycerophosphate type, di(glycerophospho)glycerol (deacylated cardiolipin) and mono ... | 1984 | 6319195 |
| survey, purification, and properties of sugar phosphate phosphohydrolase among microorganisms. | sugar phosphate phosphohydrolase was purified approximately 500- to 600-fold to apparent homogeneity from escherichia coli b, escherichia coli c, escherichia coli var. communior, escherichia acidilactici, enterobacter aerogenes, neisseria meningitidis, and saccharomyces cereviseae. the molecular weights of the enzyme as estimated by gel filtration ranged from 97 x 10(3) to 101 x 10(3). the enzyme was composed of two subunits with the same molecular weight which ranged from 50 x 10(3) to 52 x 10( ... | 1983 | 6322944 |
| regulatory regions that control expression of two chloramphenicol-inducible cat genes cloned in bacillus subtilis. | plasmid ppl603 is a promoter cloning vector for bacillus subtilis and consists of a 1.1-kilobase fragment of bacillus pumilus dna inserted between the ecori and bamhi sites of pub110. the gene cat-86, specifying chloramphenicol-inducible chloramphenicol acetyltransferase, is located on the 1.1-kilobase cloned dna. when ppl603 is present in b. subtilis, cat-86 is unexpressed during vegetative growth but expressed during sporulation. the regulation of cat-86 in ppl603 is due to sequences within tw ... | 1984 | 6327638 |
| [phenotypic expression of plasmid ppl7065 in bacillus pumilus in free and integrated states]. | there are about 20 copies of ppl7065 plasmid in cells of bacillus pumilus atcc7065 with molecular weight of 4.7 x 10(6) daltons. a spontaneous purine dependent mutant lk1 was isolated which contained no autonomous plasmid. however, the presence of the plasmid in lk1 cells was proved by dna - dna hybridization. to accomplish this, hybrid plasmid p1g1 was constructed by cloning ppl7065 on a vector pbr322 and multiplying it in escherichia coli c600. the plasmid then was hybridized with chromosomal ... | 1983 | 6343187 |
| amino acid composition and zinc content of milk-clotting protease from bacillus mesentericus strain 76. | the milk-clotting protease from bacillus mesentericus strain 76 is free of carbohydrate and lacks cysteine and cystine. the amino acid composition indicates a single peptide chain with 304 residues. five amino acids--aspartic acid, threonine, serine, glycine and alanine--represent one-half of the total residues. the enzyme contains 35 aromatic amino acids and 103 ionic amino acids. the observed constant value of the ratio menzyme:azinc is 1:1 for active and acid denatured enzyme preparations, in ... | 1983 | 6345424 |
| [purification of bacillus mesentericus proteolytic enzymes by affinity chromatography]. | by means of affinity chromatography on ovomucoid-sepharose two proteinases hydrolyzing casein and elastin were isolated from the supernatant of the bacillus mesentericus culture medium. the activity yield of proteinases was 100%. the characteristics of the purified enzymes were studied. it is demonstrated that b. mesentericus possesses several proteinases. | 1984 | 6366781 |
| studies on a membrane bound protease from bacillus pumilus & escherichia coli. | 1983 | 6373581 | |
| [multiple forms of the alkaline serine proteinase of bacillus mesentericus]. | 1983 | 6400790 | |
| chromogenic identification of genetic regulatory signals in bacillus subtilis based on expression of a cloned pseudomonas gene. | a method to isolate fragments of dna that promote gene expression in bacillus subtilis is described. the system is based on production of catechol 2,3-dioxygenase [cato2ase; catechol:oxygen 2,3-oxidoreductase (decyclizing), ec 1.13.11.2] encoded by the pseudomonas putida tol plasmid gene xyle. the gene was transferred to ab. subtilis/escherichia coli plasmid vector to construct ptg402. although xyle is functionally expressed in e. coli, cato2ase is not detected in b. subtilis unless a fragment o ... | 1983 | 6405380 |
| site-specific in vitro binding of plasmid pub110 to bacillus subtilis membrane fraction. | the in vitro membrane binding of psl103, a composite plasmid consisting of staphylococcus aureus plasmid pub110 and a bacillus pumilus trpc+ dna fragment, to the bacillus subtilis membrane fraction was studied with a total lysate of b. subtilis cells. the binding reaction required a heat treatment at 45 degrees c and had an optimum kcl concentration of 60 mm. nonradioactive psl103, but not escherichia coli plasmid pacyc184, competed with 3h-labeled psl103 for binding to the membrane. by the use ... | 1983 | 6406425 |
| [capacity of bacillus mesentericus for certain kinds of genetic exchange]. | some strains of bacillus mesentericus have an ability for transformation, transfection and transduction. the efficiency of transduction was within the same range as in bac. subtilis 168, the efficiency of transformation being significantly lower. a fragment of bac. mesentericus dna coding for indole glycerophosphate synthetase synthesis was cloned in bac. subtilis cells. in this case, manifestation of trpc2 mutation inhibiting synthesis of this enzyme in bac. subtilis, was abolished. this sugges ... | 1983 | 6413299 |
| competitive inhibition of the inverting beta-xylosidase of bacillus pumilus 12 by monosaccharide derivatives of different structural and conformational types. a possible natural substrate. | the title enzyme is competitively inhibited by compounds, for example alpha-d-xylopyranosylpyridinium salts and 1,6-anhydro-d-glucopyranose, for which the normal 4c1 conformation of the xylopyranose ring is precluded. it is competitively inhibited by compounds, for example beta-d-xylopyranosylpyridinium salts and 1,6-anhydro-l-idopyranose, for which the 1c4 conformation is precluded, and which have no accessible conformations in common with the first set of inhibitors. it is also competitively i ... | 1983 | 6414465 |
| chloramphenicol-inducible gene expression in bacillus subtilis. | a cloned bacillus pumilus cat gene expresses chloramphenicol-inducible chloramphenicol acetyltransferase activity in bacillus subtilis. the chloramphenicol inducibility trait was shown to be determined by a 234-bp region of the cloned dna. nucleotide sequence analysis of this 234-bp segment indicated that the cat ribosome-binding site occurs within a 40-bp region containing 14-bp terminal inverted repeat sequences. transcription of this region into rna should sequester the cat ribosome-binding s ... | 1983 | 6416927 |
| [cloning in bacillus subtilis cells of the bacillus mesentericus genes that determine the enzyme synthesis of tryptophan metabolism]. | in this work, we tried to clone some bacillus mesentericus genes coding for tryptophan synthesis in bacillus subtilis cells. we succeeded in obtaining two identical plasmids carrying some genes of bac. mesentericus and complementing the trpc2 and trpf mutations of bac. subtilis. the cloned genes of bac. mesentericus completely replaced the functions of trpc2 and trpf genes. | 1983 | 6418615 |
| molecular cloning of the genes for xylan degradation of bacillus pumilus and their expression in escherichia coli. | the 7.7 mdal psti fragment of bacillus pumilus ipo containing genes for xylan degradation, xylanase, and beta-xylosidase was inserted at the psti site of pbr322 and cloned in e. coli c600. the hybrid plasmid thus formed was named poxn29. the amount of xylanase and beta-xylosidase expressed in e. coli harboring poxn29 was about 6% and 20% of the activity produced by the donor, b. pumilus. the reverse orientation of the inserted fragment resulted respectively in 5 times and 50 times increases in x ... | 1983 | 6419022 |
| isolation of two beta-xylosidase genes of bacillus pumilus and comparison of their gene products. | the chromosomal dna fragments of bacillus pumilus ipo, a potent xylan-hydrolyzing bacterium, were ligated to a vector plasmid, pbr322, and used to transform escherichia coli c600 cells. two hybrid plasmids, poxd28 and poxn29, were found to enable the transformants to produce beta-xylosidase. the former was found to contain a 2.6-mda bg/ii fragment and the latter, a 7.7-mda psti fragment, both coding beta-xylosidase, but xylanase is coded only on the latter hybrid plasmid. the dnas inserted in bo ... | 1984 | 6421572 |
| the ph dependence and group modification of beta-d-xylosidase from bacillus pumilus: evidence for sulfhydryl and histidyl groups. | the ph dependence of the kinetic parameters of beta-d-xylosidase (ec. 3.2.1.37) from bacillus pumilus reveals that an acidic functional group with pk 8.0 is involved in the catalysis. the fast inactivation of the dimeric enzyme by near equivalent amounts of methylmethanethiolsulfonate indicates that one thiol group per monomer is essential for catalysis, consistent with previously reported results. from the reactivity of the thiol groups with respect to 5,5'-dithiobis(2-nitrobenzoic acid), the a ... | 1984 | 6435537 |
| [comparative study of the quantitative determination of kanamycin sulfate in ophthalmic films with a collagen base by microbiological and chemical methods]. | the results of the comparative study on microbiological and chemical quantitative determination of kanamycin sulfate in the ophthalmic films with the collagen base are presented. the intraocular films prepared with the use of 1 per cent collagen solution contain dexamethasone and kanamycin. the agar diffusion method with bacillus pumilus nctc 8241 as the test microbe and the photocolorimetric method based on estimation of the optical density of the colored compound formed after acid hydrolysis o ... | 1984 | 6524895 |
| constitutive variants of the pc194 cat gene exhibit dna alterations in the vicinity of the ribosome binding site sequence. | the chloramphenicol-inducible regulation of the expression of cat genes from two gram-positive bacteria, staphylococcus aureus and bacillus pumilus has been suggested to result from the presence of inverted repeat sequences that span the ribosome-binding site (rbs) for cat. in support of this hypothesis, we demonstrate that two derivatives of the pc194 cat gene which are constitutively expressed in bacillus subtilis are deleted for all or a major portion of the inverted-repeat sequences. | 1984 | 6588016 |