Publications

microorganisms and heavy metals associated with atmospheric deposition in a congested urban environment of a developing country: sri lanka.	the presence of bacteria and heavy metals in atmospheric deposition were investigated in kandy, sri lanka, which is a typical city in the developing world with significant traffic congestion. atmospheric deposition samples were analyzed for al, cr, mn, fe, ni, cu, zn, cd and pb which are heavy metals common to urban environments. al and fe were found in high concentrations due to the presence of natural sources, but may also be re-suspended by vehicular traffic. relatively high concentrations of ...	2017	28185730
mechanism of anti-vibrio activity of marine probiotic strain bacillus pumilus h2, and characterization of the active substance.	vibriosis is a major epizootic disease that impacts free-living and farmed fish species worldwide. use of probiotics is a promising approach for prevention of vibrio infections in aquaculture. a probiotic anti-vibrio strain, bacillus pumilus h2, was characterized, and the mechanism of its effect was investigated. all 29 vibrio strains tested were growth-inhibited by h2. the anti-vibrio substance present in cell-free supernatant of h2 was purified and characterized by reversed-phase hplc. minimum ...	2017	28097594
evaluation of bacillus cereus and bacillus pumilus metabolites for anthelmintic activity.	to assess the anthelmintic acivity of bacillus cereus and bacillus pumilus metabolites.	2017	25598639
chemical composition and biological properties of satureja avromanica maroofi.	satureja avromanica is an indigenous plant which is frequently used as a spice in avraman-kurdistan region of iran. the present study aimed to investigate the chemical composition, antimicrobial and antioxidant properties of the s. avromanica. in addition, rosmarinic acid and total phenolic content of s. avromanica was assessed by spectrophotometric method and hptlc. the essential oil and methanolic extract were isolated by hydrodistillation and maceration methods, respectively. a total of 32 co ...	2017	28298686
biocontrol potential of halotolerant bacterial chitinase from high yielding novel bacillus pumilus mcb-7 autochthonous to mangrove ecosystem.	the multifaceted role of chitinase in medicine, agriculture, environmental remediation and various other industries greatly demands the isolation of high yielding chitinase producing microorganisms with improved properties. the current study aimed to investigate the isolation, characterization and biocontrol prospective of chitinase producing bacterial strains autochthonous to the extreme conditions of mangrove ecosystems. among the 51 bacterial isolates screened, bacillus pumilus mcb-7 with hig ...	2017	28364802
insight into the bacterial diversity of fermentation woad dye vats as revealed by pcr-dgge and pyrosequencing.	the bacterial diversity in fermenting dye vats with woad (isatis tinctoria l.) prepared and maintained in a functional state for approximately 12 months was examined using a combination of culture-dependent and -independent pcr-dgge analyses and next-generation sequencing of 16s rrna amplicons. an extremely complex ecosystem including taxa potentially contributing to both indigo reduction and formation, as well as indigo degradation was found. pcr-dgge analyses revealed the presence of paenibaci ...	2017	28246965
dynamics of bacterial class bacilli in the deepest valley lake of kashmir-the manasbal lake.	in recognition of the importance of bacteria as ecological indicators of the aquatic systems a comprehensive and systematic analysis was carried out on manasbal lake, the deepest spring fed valley lake of kashmir. the main objective envisaged was to analyze bacterial community composition (bcc) and for this purpose systematic and regular sampling of waters from ten different sampling stations, predetermined in the lake according to differences in degree of human interference and also as zones of ...	2017	28087491
enhanced performance of the microalga chlorella sorokiniana remotely induced by the plant growth-promoting bacteria azospirillum brasilense and bacillus pumilus.	remote effects (occurring without physical contact) of two plant growth-promoting bacteria (pgpb) azospirillum brasilense cd and bacilus pumilus es4 on growth of the green microalga chlorella sorokiniana utex 2714 were studied. the two pgpb remotely enhanced the growth of the microalga, up to six-fold, and its cell volume by about three-fold. in addition to phenotypic changes, both bacteria remotely induced increases in the amounts of total lipids, total carbohydrates, and chlorophyll a in the c ...	2017	28145473
effects of the dietary supplementation of mixed probiotic spores of bacillus amyloliquefaciens 54a, and bacillus pumilus 47b on growth, innate immunity and stress responses of striped catfish (pangasianodon hypophthalmus).	the study used the mixed probiotics of bacillus amyloliquefaciens 54a and b. pumilus 47b isolated from striped catfish (pangasianodon hypophthalmus) intestine aiming to stimulate growth performance, innate immunity, stress tolerance of striped catfish. the average weight gain (awg), specific growth rate (sgr), and feed conversion ratio (fcr) were analyzed after fish were fed the mixture of probiotics (b. amyloliquefaciens 54a and b. pumilus 47b) at concentrations of 1 × 10(8), 3 × 10(8), and 5 × ...	2017	27836719
ribonuclease from bacillus acts as an antiviral agent against negative- and positive-sense single stranded human respiratory rna viruses.	bacillus pumilus ribonuclease (binase) was shown to be a promising antiviral agent in animal models and cell cultures. however, the mode of its antiviral action remains unknown. to assess the binase effect on intracellular viral rna we have selected single stranded negative- and positive-sense rna viruses, influenza virus, and rhinovirus, respectively, which annually cause respiratory illnesses and are characterized by high contagious nature, mutation rate, and antigen variability. we have shown ...	2017	28546965
green synthesis and characterization of gold and silver nanoparticles using mussaenda glabrata leaf extract and their environmental applications to dye degradation.	plant-derived nanomaterials opened a green approach in solving the current environment issues. present study focused on rapid microwave-assisted synthesis and applications of gold and silver nanoparticles mediated by aqueous leaf extract of mussaenda glabrata. the synthesized nanoparticles were characterized by uv-vis, ft-ir, powder xrd, energy-dispersive x-ray spectroscopy (edx), transmission electron (tem), and atomic force microscopic techniques (afm). fcc crystal structure of both nanopartic ...	2017	28589274
natural variations in the biofilm-associated protein bsla from the genus bacillus.	bsla is a protein secreted by bacillus subtilis which forms a hydrophobic film that coats the biofilm surface and renders it water-repellent. we have characterised three orthologues of bsla from bacillus amyloliquefaciens, bacillus licheniformis and bacillus pumilus as well as a paralogue from b. subtilis called ywea. we find that the three orthologous proteins can substitute for bsla in b. subtilis and confer a degree of protection, whereas ywea cannot. the degree to which the proteins function ...	2017	28751732
the profile and antimicrobial activity of bacillus lipopeptide extracts of five potential biocontrol strains.	in this study the efficacy of two different methods for extracting lipopeptides produced by five bacillus strains-ethyl acetate extraction, and acid precipitation followed by methanol extraction-was investigated using mass spectrometry. high performance thin layer chromatography (hptlc) was also used for the simultaneous separation of complex mixtures of lipopeptide extracts and for the determination of antimicrobial activity of their components. the mass spectra clearly showed well-resolved gro ...	2017	28588570
characterization of bacillus megaterium, bacillus pumilus, and paenibacillus polymyxa isolated from a pinot noir wine from western washington state.	this report provides the first confirmed evidence of bacillus-like bacteria present in a wine from washington state. these bacteria were isolated from a 2013 pinot noir wine whose aroma was sensorially described as being 'dirty' or 'pond scum.' based on physiological traits and genetic sequencing, three bacterial isolates were identified as bacillus megaterium (strain nho-1), bacillus pumilus (strain nho-2), and paenibacillus polymyxa (strain nho-3). these bacteria grew in synthetic media of low ...	2017	28648288
the role of organic acids on microbial deterioration in the radix pseudostellariae rhizosphere under continuous monoculture regimes.	a three-year field monoculture trial of radix pseudostellariae and complementary laboratory studies were conducted to further elucidate the underlying mechanism responsible for significant decreases in the biomass yield and quality of r. pseudostellariae under continuous monoculture regimes. hplc analysis indicated that continuous monoculture soil was rich in organic acids, which had cumulative effects over time. further analysis suggested that the application of a mixture of organic acids signi ...	2017	28615734
antitumor macrophage response to bacillus pumilus ribonuclease (binase).	extracellular bacterial ribonucleases such as binase from bacillus pumilus possess cytotoxic activity against tumor cells with a potential for clinical application. moreover, they may induce activation of tumor-derived macrophages either into the m1-phenotype with well-documented functions in the regulation of the antitumor immune response or into m2-macrophages that may stimulate tumor growth, metastasis, and angiogenesis. in this study, binase or endogenous rnase1 (but not rna or short oligonu ...	2017	28804220
display of b. pumilus chitinase on the surface of b. subtilis spore as a potential biopesticide.	chitinases can inhibit the growth of many fungal diseases which are a great threat for global agricultural production. biological control of pathogens like fungi, is believed to be one of the best ways to eliminate the adverse effects of plant pathogens. to this end, we expressed and displayed a chitinase from bacillus pumilus (chis) on the surface of bacillus subtilis spores, as a biocontrol agent.	2017	28755689
demodex folliculorum associated bacillus pumilus in lesional areas in rosacea.		2017	28749385
variation of preserving organic matter bound in interlayer of montmorillonite induced by microbial metabolic process.	this paper aimed to investigate the variation of preserving organic matter bound in the interlayer space of montmorillonite (mt) induced by a microbe metabolic process. we selected bacillus pumilus as the common soil native bacteria. the alteration of d 001 value, functional group, and c,n organic matter contents caused by bacteria were analyzed by xrd, ftir, and elementary analyzer, respectively. xrd results showed that the d 001 value of montmorillonite increased with the concentration decreas ...	2017	28744678
structural characterization, catalytic, kinetic and thermodynamic properties of keratinase from bacillus pumilus fh9.	bacillus pumilus fh9 keratinase was purified to homogeneity with a 59.9% yield through a series of three steps. the purified enzyme was a monomeric protein with a molecular mass around 50kda and containing 7.3% carbohydrates. the pure b. pumilus fh9 keratinase was optimally active at ph 9.0 and 60°c. the calculated activation energy for keratin hydrolysis was 24.52kjmol(-1) and its temperature quotient (q10) was 1.19. the calculated values of thermodynamic parameters for keratin hydrolysis were ...	2017	28743569
production of spore laccase from bacillus pumilus w3 and its application in dye decolorization after immobilization.	given that spore laccase from the bacillus genus is heat- and alkali-resistant, it is more suitable for industrial applications than fungal laccase. to determine the optimal culture conditions for spore laccase production, the effects of cu(2+) concentration, oxygen content, and culture time on spore laccase production from bacillus pumilus w3 were investigated. the optimal production parameters were 0.2 mm of cu(2+), 200 rpm shaking speed, 100 ml liquid loading, and 5 days of cultivation. spore ...	2017	28708619
transcriptome profiling analysis reveals metabolic changes across various growth phases in bacillus pumilus ba06.	bacillus pumilus can secret abundant extracellular enzymes, and may be used as a potential host for the industrial production of enzymes. it is necessary to understand the metabolic processes during cellular growth. here, an rna-seq based transcriptome analysis was applied to examine b. pumilus ba06 across various growth stages to reveal metabolic changes under two conditions.	2017	28693413
effective feather degradation and keratinase production by bacillus pumilus grk for its application as bio-detergent additive.	an effecient feather-degrading bacterium was isolated from poultry dumping yard and identified as bacillus pumilus grk based on 16s rrna sequencing. complete feather degradation (98.3±1.52%) with high keratinase production (373±4 u/ml) was observed in 24h under optimized conditions (substrate 1% (w/w); inoculum size 4% (v/v); ph 10; 200rpm at 37°c) with feathers as sole carbon and nitrogen source in tap water. the fermented broth was enriched with amino acids like tryptophan (221.44µg/ml), isole ...	2017	28672188
genetic determinant of bacillus pumilus lipase lethality and its application as positive selection cloning vector in escherichia coli.	positive selection vectors carry genes that upon expression produce proteins that cause host cell deaths. insertion of foreign dna fragments within the orf of the gene disrupts the lethal effect of the expressed protein. this study described the cloning of family i.4 bacillus pumilus lipase gene whose expressed protein is toxic and lethal to escherichia coli jm109 (de3) cells. the determinant of toxicity was identified through error-prone pcr to be the nature of amino acid residue resident at po ...	2017	28648403
efficacy of viable bacillus pumilus isolated from farmed fish on immune responses and increased disease resistance in nile tilapia (oreochromis niloticus): laboratory and on-farm trials.	applications of viable bacillus pumilus aqahbs01 isolated from nile tilapia farms as probiotics were studied in both laboratory and farm conditions. in the laboratory, feeding fish (approximately 50 g) with feed containing viable b. pumilus at concentrations of 1 × 10(7)-10(9) colony forming units (cfu)/kg elevated fish immune responses, as indicated by their phagocytic activity and superoxide anion levels, and led to more effective disease resistance against streptococcus agalactiae. however, w ...	2017	28602733
draft genome sequence of a porcine commensal, rothia nasimurium, encoding a nonribosomal peptide synthetase predicted to produce the ionophore antibiotic valinomycin.	we report the draft whole-genome sequence of rothia nasimurium isolated from a porcine tonsil. the genome encodes a nonribosomal peptide synthetase predicted to produce valinomycin, a cyclic dodecadepsipeptide ionophore. previously, valinomycin was known to be produced only by streptomyces species and isolates belonging to the bacillus pumilus group.	2017	28572325
bacillus pumilus strain yspmk11 as plant growth promoter and bicontrol agent against sclerotinia sclerotiorum.	a study was executed in a direction to attenuate sclerotinia stalk rot (ssr) disease through biocontrol agent and also to enhance crop productivity. culture filtrate of bacterial strain yspmk11 inhibited growth of sclerotinia sclerotiorum in vitro which also exhibited higher plant growth promoting attributes. interaction studies revealed maximum (81.50%) growth inhibition at 35 °c and ph 7.0 after 72 h incubation period with 15% culture filtrate. based upon 16s rrna gene sequence strain, yspmk11 ...	2017	28550408
insights into the mechanism of proliferation on the special microbes mediated by phenolic acids in the radix pseudostellariae rhizosphere under continuous monoculture regimes.	as potent allelochemicals, phenolic acids are believed to be associated with replanting disease and cause microflora shift and structural disorder in the rhizosphere soil of continuously monocultured radix pseudostellariae. the transcriptome sequencing was used to reveal the mechanisms underlying the differential response of pathogenic bacterium kosakonia sacchari and beneficial bacterium bacillus pumilus on their interactions with phenolic acids, the main allelochemicals in root exudates of r. ...	2017	28512464
activation and inactivation of bacillus pumilus spores by kiloelectron volt x-ray irradiation.	in this study, we investigated the inactivation efficacy of endospore-forming bacteria, bacillus pumilus, irradiated by low-energy x-rays of different beam qualities. the different low-energy x-rays studied had cut-off energies of 50, 100 and 150 kev. bacillus pumilus spores (in biological indicator strips) were irradiated at step doses between 6.5 to 390 gy. the resulting bacteria populations were then quantified by a pour plate method. results showed that x-rays of lower energies were more eff ...	2017	28493969
engineered bacillus pumilus laccase-like multi-copper oxidase for enhanced oxidation of the lignin model compound guaiacol.	laccases and laccase-like multi-copper oxidases (lmcos) are versatile and robust biocatalysts applied in a variety of oxidative processes, and various studies have attempted to improve their catalytic activity. here we report the engineering of a bacterial lmco for enhanced oxidation of the lignin-related compound guaiacol by a combination of structure-guided mutagenesis and dna shuffling. mutant l9 showed a 1.39 mm km for guaiacol and a 2.5-fold increase in turnover rate (kcat/km = 2.85·104 m-1 ...	2017	28482039
growth inhibition and antibiofilm potential of ag nanoparticles coated with lectin, an arthropod immune molecule.	lectins from the haemolymph of arthropods, including crustaceans, are molecules potentially involved in the immune recognition and phagocytosis. here, lectin was purified from the haemolymph of blue swimmer crab portunus pelagicus, using mannose-coupled sepharose cl-4b affinity column chromatography. in sds-page analysis, lectin showed a molecular mass of approximately 155kda. the synthesis of lectin-coated silver nanoparticles (lectin-coated agnps) was confirmed by uv-vis spectroscopy, xrd, fti ...	2017	28441606
the curious case of achromobacter eurydice, a gram-variable pleomorphic bacterium associated with european foulbrood disease in honeybees.	honeybees are prone to parasite and pathogen infestations/infections due to their social colony life. bacterial pathogens in particular lead to destructive infections of the brood. european foulbrood is caused by the bacterium melissococcus plutonius in combination with several other gram-positive bacteria (achromobacter eurydice, bacillus pumilus, brevibacillus laterosporus, enterococcus faecalis, paenibacillus alvei, paenibacillus dendritiformis) involved as secondary invaders following the in ...	2017	28634639
dataset on potential large scale production of biosurfactant using bacillus sp.	surfactants are very important in industry. the cost of commercial surfactant production is still high and the surfactant demand is constantly increasing. microbial production of surfactant known as biosurfactant shows commercial potency. utilization of bacillus sp. strain on glucose fermentation for biosurfactant production was then studied. this type of microbe was isolated from soil contaminated with palm oil. the selection of the strain was based on its ability to form emulsifying zone aroun ...	2017	28616451
seasonal and regional occurrence of heat-resistant spore-forming bacteria in the course of ultra-high temperature milk production in tunisia.	spore-forming bacteria, principally bacillus species, are important contaminants of milk. because of their high heat resistance, bacillus species spores are capable of surviving the heat treatment process of milk and lead to spoilage of the final product. to determine the factors influencing the contamination of milk, spore-forming bacteria occurrence throughout the uht milk production line during winter, spring, and summer was studied. the obtained results confirm that the total viable rate dec ...	2017	28571988
biopolymer zein-coated gold nanoparticles: synthesis, antibacterial potential, toxicity and histopathological effects against the zika virus vector aedes aegypti.	the control of multidrug-resistant bacteria as well as insect pests and vectors is timely and important now a days. the present study was designed to evaluate the in vitro antibacterial, antibiofilm and mosquito larvicidal effects of gold nanoparticles synthesized using the zein biopolymer (ze-aunps) against gram positive (bacillus pumilus and bacillus subtilis), gram negative (shigella sonnei and pseudomonas aeruginosa) bacteria and third instar larvae of the dengue and zika virus vector aedes ...	2017	28654862
novel antimicrobial activities of a peptide derived from a japanese soybean fermented food, natto, against streptococcus pneumoniae and bacillus subtilis group strains.	we recently isolated a tumoricidal peptide from natto, a japanese traditional fermented food. in the present study, antimicrobial activity of the natto peptide was examined. the peptide consisted of 45 amino acid residues, and its structure was predicted to be rich in α-helix. it excreted antimicrobial activity only against streptococcus pneumoniae and bacillus subtilis group (b. subtilis, bacillus pumilus, and bacillus licheniformis). lesser antimicrobial activity was observed for streptococcus ...	2017	28641406
bacillus pumilus katx2 confers enhanced hydrogen peroxide resistance to a bacillus subtilis pkata::katx2 mutant strain.	bacillus pumilus cells exhibit a significantly higher resistance to hydrogen peroxide compared to closely related bacilli like bacillus subtilis.	2017	28446175
production of polyhydroxyalkanoates (pha) using sludge from different wastewater treatment processes and the potential for medical and pharmaceutical applications.	in this study, seven strains of bacteria with polyhydroxyalkanoates (pha)-producing ability (i.e., bacillus cereus, pseudomonas putida, bacillus pumilus, pseudomona-huttiensis, yersinia-frederiksenii, aeromonas ichthiosmia and sphingopyxis terrae) were isolated from various waste treatment plants in hong kong. simultaneous wastewater treatment and pha accumulation were successfully achieved in the bioreactors using isolated bacteria from different sludges. at the organic loading less than 13000 ...	2017	28387154
green synthesis of rifampicin-loaded copper nanoparticles with enhanced antimicrobial activity.	the antimicrobial properties of copper and rifampicin-loaded copper nanoparticles were investigated using four strains: staphylococcus aureus, escherichia coli, bacillus pumilis and pseudomonas fluorescens. spherical-shaped copper nanoparticles were synthesized via green reduction method from the peppermint extract. it was found that adsorption of rifampicin on the copper nanosurface enhances its biological activity and prevents the development of resistance. the interactions between rifampicin- ...	2017	28150115