Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| isolation and characterization of two novel psychrotrophic decabromodiphenyl ether-degrading bacteria from river sediments. | decabromodiphenyl ether (bde-209) is a brominated flame retardant and a priority contaminant. currently, little information is available about its significance in the environment, specifically about its susceptibility to aerobic biotransformation at low temperature. in this work, five phylogenetically diverse bde-209-degrading bacterial strains were isolated from river sediments of northern china. these strains were distributed among four different genera-acinetobacter, pseudomonas, bacillus and ... | 2016 | 26517991 |
| chitin from penaeus merguiensis via microbial fermentation processing and antioxidant activity. | the ability of three proteases producing bacteria such as pseudomonas aeruginosa, serratia marcescens, and bacillus pumilus on the demineralization and deproteinzation efficiency of shrimp waste, for chitin extraction, was investigated. statistical analysis of data was showed a significant difference between the percentage of demineralization and deproteinization in different bacteria species (p<0.05). the highest deproteinization (74.76%) and demineralization rate (78.46%) was obtained with p. ... | 2016 | 26519877 |
| detection of boron removal capacities of different microorganisms in wastewater and effective removal process. | in this study boron removal capacities of different microorganisms were tested. candida tropicalis, rhodotorula mucilaginosa, micrococcus luteus, bacillus thuringiensis, bacillus cereus, bacillus megaterium, bacillus pumilus, pseudomonas aeruginosa and aspergillus versicolor were examined for their boron bioaccumulation capacities in simulated municipal wastewater. a. versicolor and b. cereus were found as the most boron-tolerant microorganisms in the experiments. also boron bioaccumulation yiel ... | 2015 | 26540546 |
| heterologous expression of mannanase and developing a new reporter gene system in lactobacillus casei and escherichia coli. | reporter gene systems are useful for studying bacterial molecular biology, including the regulation of gene expression and the histochemical analysis of protein products. here, two genes, β-1,4-mannanase (manb) from bacillus pumilus and β-glucuronidase (gusa) from escherichia coli k12, were cloned into the expression vector pelx1. the expression patterns of these reporter genes in lactobacillus casei were investigated by measuring their enzymatic activities and estimating their recombinant prote ... | 2015 | 26562012 |
| sensitive change of iso-branched fatty acid (iso-15:0) in bacillus pumilus pamc 23174 in response to environmental changes. | in this study, the environmental adaptive metabolic processes were investigated using a psychrotrophic polar bacterium bacillus pumilus pamc 23174 in response to various temperatures and nutrients, especially in regard to the synthesis of fatty acids. fatty acid methyl ester analysis was performed using gas chromatography-mass spectrometry and we found that a sensitive changes in iso-branched fatty acid (iso-15:0) synthesis occurred when adjusting the nutritional ratio of branched chain fatty ac ... | 2016 | 26566954 |
| identification of bacillus strains by maldi tof ms using geometric approach. | microorganism identification by maldi tof mass-spectrometry is based on the comparison of the mass spectrum of the studied organism with those of reference strains. it is a rapid and reliable method. however, commercial databases and programs are mostly designed for identification of clinically important strains and can be used only for particular mass spectrometer models. the need for open platforms and reference databases is obvious. in this study we describe a geometric approach for microorga ... | 2015 | 26592761 |
| bacillus zhangzhouensis sp. nov. and bacillus australimaris sp. nov. | two strains, designated dw5-4t and nh7i_1t, were isolated from aquaculture water of shrimp farm of zhangzhou city in china and surface sediment of the south china sea, respectively. cells of the strains were gram-stain-positive, oxidase- and catalase-positive, rod-shaped and motile. the phylogenetic analyses based on 16s rrna gene sequences indicated that two strains belonged to the bacillus pumilus clade. the similarities of 16s rrna gene sequences between two strains dw5-4t and nh7i_1t was 99. ... | 2015 | 26705002 |
| catalytic, kinetic and thermodynamic properties of bacillus pumilus fh9 keratinase conjugated with activated pectin. | bacillus pumilus fh9 keratinase was covalently coupled to several oxidized polysaccharides. the conjugates were evaluated for the retained activity, kinetic and thermodynamic stability. among all preparations, the conjugated enzyme with oxidized pectin had the highest recovered activity (71.75%) and the highest thermal stability at 60°c (t1/2=333 min). compared to the native enzyme, the conjugated preparation exhibited higher optimum temperature, lower activation energy (ea), lower deactivation ... | 2016 | 26743746 |
| probing an interfacial surface in the cyanide dihydratase from bacillus pumilus, a spiral forming nitrilase. | nitrilases are of significant interest both due to their potential for industrial production of valuable products as well as degradation of hazardous nitrile-containing wastes. all known functional members of the nitrilase superfamily have an underlying dimer structure. the true nitrilases expand upon this basic dimer and form large spiral or helical homo-oligomers. the formation of this larger structure is linked to both the activity and substrate specificity of these nitrilases. the sequences ... | 2015 | 26779137 |
| aerobic and anaerobic microbial degradation of crude (4-methylcyclohexyl)methanol in river sediments. | cyclohexane and some of its derivatives have been a major concern because of their significant adverse human health effects and widespread occurrence in the environment. the 2014 west virginia chemical spill has raised public attention to (4-methylcyclohexyl)methanol (4-mchm), one cyclohexane derivative, which is widely used in coal processing but largely ignored. in particular, the environmental fate of its primary components, cis- and trans-4-mchm, remains largely unexplored. this study aimed ... | 2016 | 26780132 |
| an in-depth characterization of the entomopathogenic strain bacillus pumilus 15.1 reveals that it produces inclusion bodies similar to the parasporal crystals of bacillus thuringiensis. | in the present work, the local isolate bacillus pumilus 15.1 has been morphologically and biochemically characterized in order to gain a better understanding of this novel entomopathogenic strain active against ceratitis capitata. this strain could represent an interesting biothechnological tool for the control of this pest. here, we report on its nutrient preferences, extracellular enzyme production, motility mechanism, biofilm production, antibiotic suceptibility, natural resistance to chemica ... | 2016 | 26782747 |
| purification and partial characterization of serine-metallokeratinase from a newly isolated bacillus pumilus nrc21. | a serine metallokeratinase enzyme (30 kda) produced by a newly isolated bacillus strain (bacillus pumilus nrc21) cultivated under optimized conditions in medium containing chicken feather meal was purified and characterized in a set of biochemical assays. the purification was carried out using two successive chromatographic steps; cation exchange chromatography on cm-cellulose and gel filtration on sephadex g-100 columns. the purified enzyme showed a specific activity of 2000 units/mg protein ag ... | 2016 | 26802243 |
| applicability of uv resistant bacillus pumilus endospores as a human adenovirus surrogate for evaluating the effectiveness of virus inactivation in low-pressure uv treatment systems. | recent studies have demonstrated the potential to use bacillus pumilus endospores as a surrogate for human adenovirus (hadv) in uv disinfection studies. the use of endospores has been limited by observations of batch-to-batch variation in uv sensitivity. this study reports on a propagation method that utilizes a commercially available medium to produce uv tolerant b. pumilus endospores with a consistent uv sensitivity. it is further demonstrated that the endospores of b. pumilus strain (atcc 271 ... | 2016 | 26825005 |
| cell wall as a target for bacteria inactivation by pulsed electric fields. | the integrity and morphology of bacteria is sustained by the cell wall, the target of the main microbial inactivation processes. one promising approach to inactivation is based on the use of pulsed electric fields (pef). the current dogma is that irreversible cell membrane electro-permeabilisation causes the death of the bacteria. however, the actual effect on the cell-wall architecture has been poorly explored. here we combine atomic force microscopy and electron microscopy to study the cell-wa ... | 2016 | 26830154 |
| thermostable xanthine oxidase activity from bacillus pumilus rl-2d isolated from manikaran thermal spring: production and characterization. | xanthine oxidase is an important enzyme of purine metabolism that catalyzes the hydroxylation of hypoxanthine to xanthine and then xanthine to uric acid. a thermostable xanthine oxidase is being reported from a thermophilic organism rl-2d isolated from the manikaran (kullu) hot spring of himachal pradesh (india). based on the morphology, physiological tests, and 16s rdna gene sequence, rl-2d was identified as bacillus pumilus. optimization of physiochemical parameters resulted into 4.1-fold incr ... | 2016 | 26843701 |
| analysis of bacterial communities associated with potting media. | potting media are commonly used by growers in different parts of the world for potted plants, raising seedlings and for improving soil characteristics. this study was conducted to characterize bacterial communities occurring in 13 commercial potting media products originating from seven countries. | 2016 | 26844021 |
| regioselective deprotection of the monosaccharide-bearing thiocyanomethyl group at the anomeric position monitored by reversed-phase hplc method. | in the current work, the investigation and development of a chemo-enzymatic approach for the synthesis of neo-glycoproteins have been studied. this strategy is based on the regioselective enzymatic hydrolysis of peracetylated monosaccharide, functionalized at the anomeric position (c1) as 1-thio-(s-cyanomethyl) group, a precursor of the 2- iminomethoxyethyl thioglycosides-linker for protein glycosylation, catalyzed by immobilized enzymes to obtain selectively monodeprotected compounds. the use o ... | 2016 | 26864255 |
| genome sequence of bacillus pumilus strain bonn, isolated from an anthrax-like necrotic skin infection site of a child. | we report the draft genome sequence of bacillus pumilus strain bonn associated with human skin infection. b. pumilus bonn was isolated from a carbuncle-like necrotic site, resembling cutaneous anthrax, on the back of the hand of a 10-year-old child. | 2016 | 26868410 |
| construction and characterization of three protein-targeting expression system in lactobacillus casei. | we previously reported that the β-1,4-mannanase (manb) gene from bacillus pumilus functions as a good reporter gene in lactobacillus casei. two vectors were constructed. one carries the signal peptide of secretion protein usp45 (spusp45) from lactococcus lactis (pelsh), and the other carries the full-length s-layer protein, slpa, from l. acidophilus (pelwh). in this work, another vector, pelsph, was constructed to include the signal peptide of protein slpa (spslpa), and the capacity of all three ... | 2016 | 26892019 |
| identification of bacillus probiotics isolated from soil rhizosphere using 16s rrna, reca, rpob gene sequencing and rapd-pcr. | some bacillus species, especially bacillus subtilis and bacillus pumilus groups, have highly similar 16s rrna gene sequences, which are hard to identify based on 16s rdna sequence analysis. to conquer this drawback, rpob, reca sequence analysis along with randomly amplified polymorphic (rapd) fingerprinting was examined as an alternative method for differentiating bacillus species. the 16s rrna, rpob and reca genes were amplified via a polymerase chain reaction using their specific primers. the ... | 2016 | 26898909 |
| cost-effective and concurrent production of industrially valuable xylano-pectinolytic enzymes by a bacterial isolate bacillus pumilus ajk. | simultaneous production of xylanase and pectinase by bacillus pumilus ajk under submerged fermentation was investigated in this study. under optimized conditions, it produced 315 ± 16 iu/ml acidic xylanase, 290 ± 20 iu/ml alkaline xylanase, and 88 ± 9 iu/ml pectinase. the production of xylano-pectinolytic enzymes was the highest after inoculating media (containing 2% each of wheat bran and citrus limetta peel, 0.5% peptone, 10 mm mgso4, ph 7.0) with 2% of 21-hr-old culture and incubated at 37°c ... | 2017 | 26914524 |
| [alkaline-adapted beta-mannanase of bacillus pumilus: gene heterologous expression and enzyme characterization]. | we expressed a novel alkaline-adapted beta-mannanase gene and characterized the enzyme for potential industrial applications. | 2015 | 26915226 |
| growth and (137)cs uptake and accumulation among 56 japanese cultivars of brassica rapa, brassica juncea and brassica napus grown in a contaminated field in fukushima: effect of inoculation with a bacillus pumilus strain. | fifty six local japanese cultivars of brassica rapa (40 cultivars), brassica juncea (10 cultivars) and brassica napus (6 cultivars) were assessed for variability in growth and (137)cs uptake and accumulation in association with a bacillus pumilus strain. field trial was conducted at a contaminated farmland in nihonmatsu city, in fukushima prefecture. inoculation resulted in different responses of the cultivars in terms of growth and radiocesium uptake and accumulation. b. pumilus induced a signi ... | 2016 | 26986237 |
| [inhibition of bacillus pumilus ar03 on alternaria alternata and erysiphe cichoracearum on tobacco]. | this study aimed to evaluate the efficiency of bacillus pumilus ar03 against altenaria alternata and erysiphe cichoracearum. the antagonistic activities were studied in the way of co-culture on plate, inhibition of pathogen conidia germination on concave slides and lb agar medium. in the investigation, the water suspension of living cells of strain ar03, at 3 x 10(8) cfu . ml-1 had a remarkable inhibition effect on hyphae growth and conidia germination of a. alternata and caused hyphae deformati ... | 2015 | 26995927 |
| plant growth-promoting rhizobacteria inoculation to enhance vegetative growth, nitrogen fixation and nitrogen remobilisation of maize under greenhouse conditions. | plant growth-promoting rhizobacteria (pgpr) may provide a biological alternative to fix atmospheric n2 and delay n remobilisation in maize plant to increase crop yield, based on an understanding that plant-n remobilisation is directly correlated to its plant senescence. thus, four pgpr strains were selected from a series of bacterial strains isolated from maize roots at two locations in malaysia. the pgpr strains were screened in vitro for their biochemical plant growth-promoting (pgp) abilities ... | 2016 | 27011317 |
| mixed phenolic acids mediated proliferation of pathogens talaromyces helicus and kosakonia sacchari in continuously monocultured radix pseudostellariae rhizosphere soil. | radix pseudostellariae l. is a common and popular chinese medication. however, continuous monoculture has increased its susceptibility to severe diseases. we identified two pathogenic microorganisms, talaromyces helicus m. (ku355274) and kosakonia sacchari w. (ku324465), and their antagonistic bacterium, bacillus pumilus z. in rhizosphere soil of continuously monocultured r. pseudostellariae. nine types of phenolic acids were identified both in the rhizosphere soil and in culture medium under st ... | 2016 | 27014250 |
| heterologous expression and characterization of a new heme-catalase in bacillus subtilis 168. | reactive oxygen species (ros) is an inherent consequence to all aerobically living organisms that might lead to the cells being lethal and susceptible to oxidative stress. bacillus pumilus is characterized by high-resistance oxidative stress that stimulated our interest to investigate the heterologous expression and characterization of heme-catalase as potential biocatalyst. results indicated that recombinant enzyme significantly exhibited the high catalytic activity of 55,784 u/mg expressed in ... | 2016 | 27016935 |
| identification and pathogenic potential of clinical bacillus and paenibacillus isolates. | the soil-related bacillus and paenibacillus species have increasingly been implicated in various human diseases. nevertheless, their identification still poses problems in the clinical microbiology laboratory and, with the exception of bacillus anthracis and bacillus cereus, little is known on their pathogenicity for humans. in this study, we evaluated the use of matrix-assisted laser desorption-ionization time of flight mass spectrometry (maldi-tof ms) in the identification of clinical isolates ... | 2016 | 27031639 |
| direct inhibition of oncogenic kras by bacillus pumilus ribonuclease (binase). | ras proteins function as molecular switches that transmit signals from cell surface receptors into specific cellular responses via activation of defined signaling pathways (fang, 2015). aberrant constitutive ras activation occurs with high incidence in different types of cancer (bos, 1989). thus, inhibition of ras-mediated signaling is extremely important for therapeutic approaches against cancer. here we showed that the ribonuclease (rnase) binase, directly interacts with endogenous kras. furth ... | 2016 | 27066977 |
| diversity of indigenous endophytic bacteria associated with the roots of chinese cabbage (brassica campestris l.) cultivars and their antagonism towards pathogens. | the study aimed to reveal the diversity of endophytic bacteria in the roots of chinese cabbage (cc) cultivated in two areas in korea, namely, seosang-gun (ss) and haenam-gun (hn), and also in a transgenic plant (tp) from the laboratory. a total of 653 colonies were isolated from the interior of cc roots, comprising 118, 302, and 233 isolates from ss, hn, and tp samples, respectively. based on 16s rrna gene sequence analysis, the isolates belonged to four major phylogenetic groups: high-g+c gram- ... | 2016 | 27095454 |
| [characterization of β-1, 4-mannanase from bacillus pumilus and heterologous expression in lactobacillus casei]. | lactobacillus casei is widely used in food production and feed industry. the aim of this study was to construct the recombinant expression mannanase lb. casei. | 2015 | 27101700 |
| identification of phenazine-1-carboxylic acid gene (phc cd) from bacillus pumilus mtcc7615 and its role in antagonism against rhizoctonia solani. | bacillus pumilus mtcc7615, a biocontrol agent isolated from rice rhizosphere was characterized to be antagonistic to rhizoctonia solani, the pathogen causing sheath blight disease of rice. the phenazine-1-carboxylic acid gene (phc cd) of this bacterium was pcr amplified (1400 bp), cloned, and sequenced. the sequence analysis revealed the presence of two orfs of phc cd gene commonly found in pseudomonas species. the sequence showed 98% similarity to phc cd gene of the pseudomonas isolate lbum223 ... | 2016 | 27106067 |
| a bacillus sp. isolated from sediments of the sarno river mouth, gulf of naples (italy) produces a biofilm biosorbing pb(ii). | a pb-resistant bacterial strain (named hereinafter pb15) has been isolated from highly polluted marine sediments at the sarno river mouth, italy, using an enrichment culture to which pb(ii) 0.48mmoll(-1) were added. 16s rrna gene sequencing (sanger) allowed assignment of the isolate to the genus bacillus, with bacillus pumilus as the closest species. the isolate is resistant to pb(ii) with a minimum inhibitory concentration (mic) of 4.8mmoll(-1) and is also resistant to cd(ii) and mn(ii) with mi ... | 2016 | 27110973 |
| cationic antimicrobial peptides derived from crocodylus siamensis leukocyte extract, revealing anticancer activity and apoptotic induction on human cervical cancer cells. | known antimicrobial peptides kt2 and rt2 as well as the novel rp9 derived from the leukocyte extract of the freshwater crocodile (crocodylus siamensis) were used to evaluate the ability in killing human cervical cancer cells. rp9 in the extract was purified by a combination of anion exchange column and reversed-phase hplc, and its sequence was analyzed by mass spectrometry. the novel peptide could inhibit gram-negative vibrio cholerae (clinical isolation) and gram-positive bacillus pumilus tistr ... | 2016 | 27129462 |
| digital data for quick response (qr) codes of alkalophilic bacillus pumilus to identify and to compare bacilli isolated from lonar crator lake, india. | microbiologists are routinely engaged isolation, identification and comparison of isolated bacteria for their novelty. 16s rrna sequences of bacillus pumilus were retrieved from ncbi repository and generated qr codes for sequences (fasta format and full gene bank information). 16srrna were used to generate quick response (qr) codes of bacillus pumilus isolated from lonar crator lake (19° 58' n; 76° 31' e), india. bacillus pumilus 16s rrna gene sequences were used to generate cgr, fcgr and pca. t ... | 2016 | 27141529 |
| plantazolicin is an ultra-narrow spectrum antibiotic that targets the bacillus anthracis membrane. | plantazolicin (pzn) is a ribosomally synthesized and post-translationally modified natural product from bacillus methylotrophicus fzb42 and bacillus pumilus. extensive tailoring to twelve of the fourteen amino acid residues in the mature natural product endows pzn with not only a rigid, polyheterocyclic structure, but also antibacterial activity. here we report a remarkably discriminatory activity of pzn toward bacillus anthracis, which rivals a previously-described gamma (γ) phage lysis assay i ... | 2016 | 27152321 |
| a phosphate starvation-inducible ribonuclease of bacillus licheniformis. | the bli03719 protein of bacillus licheniformis dsm13 belongs to the most abundant extracellular proteins under phosphate starvation conditions. in this study, the function of this phosphate starvation inducible protein was determined. an amino-acid sequence analysis of the bli03719-encoding gene showed a high similarity with genes encoding the barnase of bacillus amyloliquefaciens fzb42 and binase-like rnase of bacillus pumilus sarf-032. the comparison of the control strain and a bli03719-defici ... | 2016 | 27160576 |
| increasing the catalytic activity of bilirubin oxidase from bacillus pumilus: importance of host strain and chaperones proteins. | aggregation of recombinant proteins into inclusion bodies (ibs) is the main problem of the expression of multicopper oxidase in escherichia coli. it is usually attributed to inefficient folding of proteins due to the lack of copper and/or unavailability of chaperone proteins. the general strategies reported to overcome this issue have been focused on increasing the intracellular copper concentration. here we report a complementary method to optimize the expression in e. coli of a promising bilir ... | 2016 | 27165502 |
| bacterial biota of nigeen lake waters (kashmir valley). | one of the greatest apprehensions of water consumers all over the world with respect to the quality of drinking water is its contamination with pathogenic microorganisms. this research work determined the potential bacterial contaminants of the waters of nigeen lake, a subsidiary of dal lake and is regarded as a separate lake in kashmir. the study was carried out from may 2014 to november 2014 excluding august and september at four different sites. during the study the bacterial flora showed var ... | 2016 | 27165539 |
| anthracnose of lucky bamboo dracaena sanderiana caused by the fungus colletotrichum dracaenophilum in egypt. | dracaena sanderiana, of the family liliaceae, is among the ornamental plants most frequently imported into egypt. typical anthracnose symptoms were observed on the stems of imported d. sanderiana samples. the pathogen was isolated, demonstrated to be pathogenic based on koch's rule and identified as colletotrichum dracaenophilum. the optimum temperature for its growth ranges from 25 to 30 °c, maintained for 8 days. kemazed 50% wettable powder (wp) was the most effective fungicide against the pat ... | 2016 | 27222738 |
| optimal secretion of alkali-tolerant xylanase in bacillus subtilis by signal peptide screening. | xylanases are industrially important enzymes for xylan digestion. we experimentally screened over 114 sec and 24 tat pathway signal peptides, with two different promoters, for optimal production of an alkaline active xylanase (xynbyg) from bacillus pumilus byg in a bacillus subtilis host. though both promoters yielded highly consistent secretion levels (0.97 pearson correlation coefficient), the sec pathway was found to be more efficient than the tat pathway for xynbyg secretion. furthermore, th ... | 2016 | 27225471 |
| cloning and expression of dnak gene from bacillus pumilus of hot water spring origin. | a set of thermotolerant strains isolated from hot springs of manikaran and bakreshwar (india) were selected with an aim to isolate dnak gene which encodes dnak protein. the gene dnak along with its flanking region was successfully amplified from 5 different strains (4 from bakreshwar and one from manikaran). restriction fragment length polymorphism (rflp) revealed that amplicons were almost identical in sequence. the dnak gene from one representative, bacillus pumilus strain b3 isolated from bak ... | 2013 | 27275408 |
| two-stage phyto-microremediation of tannery effluent by spirodela polyrrhiza (l.) schleid. and chromium resistant bacteria. | two-stage sequential treatment of tannery effluent was conducted employing a wetland plant, spirodela polyrrhiza (l.) schleid., and chromium (cr) resistant bacterial strains. the bacterial strains were isolated from cr-enriched environmental matrices and rhizosphere of spirodela polyrrhiza. the phyto-rhizoremediation of tannery effluent by spirodela and its rhizospheric bacteria (cellulomonas biazotea apbr1-6, bacillus safensis apbr2-12, staphylococcus warneri apbr3-5, microbacterium oleivorans ... | 2016 | 27318662 |
| isolation, identification, and optimal cultivation of a marine bacterium antagonistic to magnaporthe grisea. | in this paper, a plate confrontation method was used to isolate bacteria antagonistic to the rice blast fungus magnaporthe grisea from samples collected from china's dalian bay. the antagonist strain lm-031 was obtained. we studied this strain's morphological, physiological and biochemical characteristics and analyzed its 16s rdna sequence. we compared the effects of different culture conditions (type of media, carbon and nitrogen source, incubation temperature and time, and initial ph value) on ... | 2016 | 27323038 |
| bacillus pumilus safr-032 genome revisited: sequence update and re-annotation. | bacillus pumilus strain safr-032 is a non-pathogenic spore-forming bacterium exhibiting an anomalously high persistence in bactericidal environments. in its dormant state, it is capable of withstanding doses of ultraviolet (uv) radiation or hydrogen peroxide, which are lethal for the vast majority of microorganisms. this unusual resistance profile has made safr-032 a reference strain for studies of bacterial spore resistance. the complete genome sequence of b. pumilus safr-032 was published in 2 ... | 2016 | 27351589 |
| identification of two new keratinolytic proteases from a bacillus pumilus strain using protein analysis and gene sequencing. | the bacillus strain (ccug 66887) has a high capacity to excrete keratinase with the ability to degrade both alpha- and beta keratin. in this study we aimed to show the characteristics of the keratinolytic protease and to identify its gene by using liquid chromatography-electrospray ionization tandem mass spectrometry methods (nanohplc-esi-ms/ms) followed by mascot data base search. the results showed that the enzyme in fact consists of two different keratinases, both with a molecular mass of 38 ... | 2016 | 27363997 |
| bacillus pumilus septic arthritis in a healthy child. | we report a case of septic arthritis caused by a bacillus species, b. pumilus, occurring in a healthy child. this organism rarely causes serious infections and has only been described in newborns and immunocompromised individuals or as a skin infection. this child developed an indolent joint swelling after a minor skin injury, and symptoms were initially thought most consistent with chronic arthritis. the case demonstrates that clinicians should consider joint infection in children presenting wi ... | 2016 | 27366165 |
| [combined action of binase and bleomycin toward human lung adenocarcinoma cells]. | some microbial ribonucleases (rnases) demonstrate selective cytotoxic effect against a wide range of tumor cells. in this context combined use of cytotoxic rnases in complex therapy with other chemotherapeutic agents appears to be especially promising. in this study we have investigated the apoptosis-induced effect of bacillus pumilus rnase (binase) in combination with known anti-tumor antibiotic bleomycin on human lung adenocarcinoma a549 cells. the combined effect of high concentrations of the ... | 2016 | 27420619 |
| affinity-driven immobilization of proteins to hematite nanoparticles. | functional nanoparticles are valuable materials for energy production, bioelectronics, and diagnostic devices. the combination of biomolecules with nanosized material produces a new hybrid material with properties that can exceed the ones of the single components. hematite is a widely available material that has found application in various sectors such as in sensing and solar energy production. we report a single-step immobilization process based on affinity and achieved by genetically engineer ... | 2016 | 27429157 |
| draft genome sequence of bacillus pumilus ku-bf1 isolated from the gut contents of wood boring mesomorphus sp. | 2016 | 27446065 | |
| antimicrobial activities of bacteria associated with the brown alga padina pavonica. | macroalgae belonging to the genus padina are known to produce antibacterial compounds that may inhibit growth of human- and animal pathogens. hitherto, it was unclear whether this antibacterial activity is produced by the macroalga itself or by secondary metabolite producing epiphytic bacteria. here we report antibacterial activities of epiphytic bacteria isolated from padina pavonica (peacocks tail) located on northern coast of tunisia. eighteen isolates were obtained in pure culture and tested ... | 2016 | 27462308 |
| effect of metal ions and petrochemicals on bioremediation of chlorpyrifos in aerobic sequencing batch bioreactor (asbr). | application of chlorpyrifos (cp) has increased its environmental concentration. increasing cp concentration has increased chances of adverse health effects. its removal from environment has attained researcher's attention. cp degrading bacterial strains were isolated from wastewater and agricultural soil. finally, selected five bacterial strains were identified using 16s rrna nucleotide sequence analysis as pseudomonas kilonensis srk1, serratia marcescens srk2, bacillus pumilus srk4, achromobact ... | 2016 | 27470246 |
| extracellular proteome profiling of bacillus pumilus scu11 producing alkaline protease for dehairing. | bacillus pumilus is one of the most characterized microorganisms that are used for high-level production of select industrial enzymes. a novel b. pumilus scu11 strain possessing high alkaline protease activity was obtained in our previous work. the culture supernatant of this strain showed efficient dehairing capability with minimal collagen damage, indicating promising potential applications in the leather industry. in this study, the strain's extracellular proteome was identified by lc-ms/ms-b ... | 2016 | 27470280 |
| development of an efficient bacterial consortium for the potential remediation of hydrocarbons from contaminated sites. | the intrinsic biodegradability of hydrocarbons and the distribution of proficient degrading microorganisms in the environment are very crucial for the implementation of bioremediation practices. among others, one of the most favorable methods that can enhance the effectiveness of bioremediation of hydrocarbon-contaminated environment is the application of biosurfactant producing microbes. in the present study, the biodegradation capacities of native bacterial consortia toward total petroleum hyd ... | 2016 | 27471499 |
| transfer of radiocesium from rhizosphere soil to four cruciferous vegetables in association with a bacillus pumilus strain and root exudation. | this study was carried out to assess the effect of bacillus pumilus on the roots of four cruciferous vegetables with different root structures in regard to enhancement of (137)cs bioavailability in contaminated rhizosphere soil. results revealed that b. pumilus inoculation did not enhance the plant biomass of vegetables, although it increased root volume and root surface areas of all vegetables except turnip. the ph changes due to rhizosphere acidification by b. pumilus inoculation and root exud ... | 2016 | 27517724 |
| speciation of bacillus spp. in honey produced in northern ireland by employment of 16s rdna pcr and automated dna sequencing techniques. | phenotypic speciation of foodborne bacillus spp. remains problematic in terms of obtaining a reliable identification. in this study, we wished to identify several bacterial isolates from honey produced in northern ireland, and which belonged to the genus bacillus, through employment of a molecular identification scheme based on pcr amplification of universal regions of the 16s rrna operon in combination with direct automated sequencing of the resulting amplicons. seven samples of honey and relat ... | 2007 | 27517837 |
| [a sepsis case caused by a rare opportunistic pathogen: bacillus pumilus]. | the high prevalence of bacillus species in nature and the detection of these bacteria as contaminant in cultures may lead diagnostic dilemma, however they should still be considered as a pathogen particularly in case of repeated positive cultures from patients with risk factors. bacillus pumilus is a bacteria, though rarely, been reported as the causative agent of various infections such as sepsis, endocarditis, skin infections and food poisoning in human. in this report, a sepsis case in an imm ... | 2016 | 27525402 |
| increased mrna expression of selected antimicrobial peptides around ovulation and during inflammatory processes in the bovine endometrium postpartum. | in the uterus, the first pathogen confrontations take place at the luminal endometrial epithelium. therefore, it is required that these cells have the potential to recognize and respond to a bacterial infection. antimicrobial peptides (amp), part of the innate immune system in addition to cytokines, are principal effector molecules of mucosal immunity against pathogens. one important family of amp that can permeabilize bacterial membranes is the beta-defensin (defb) family, which includes the fo ... | 2016 | 27531713 |
| how the intricate interactions between carbon nanotubes and two bilirubin oxidases control direct and mediated o2 reduction. | due to the lack of a valid approach in the design of electrochemical interfaces modified with enzymes for efficient catalysis, many oxidoreductases are still not addressed by electrochemistry. we report in this work an in-depth study of the interactions between two different bilirubin oxidases, (from the fungus myrothecium verrucaria and from the bacterium bacillus pumilus), catalysts of oxygen reduction, and carbon nanotubes bearing various surface charges (pristine, carboxylic-, and pyrene-met ... | 2016 | 27533778 |
| effect of oral administration of bacillus coagulans b37 and bacillus pumilus b9 strains on fecal coliforms, lactobacillus and bacillus spp. in rat animal model. | to investigate the effect of oral administration of two bacillus strains on fecal coliforms, lactobacillus and bacillus spp. in rat animal model. | 2016 | 27536040 |
| an alkaline protease from bacillus pumilus mp 27: functional analysis of its binding model toward its applications as detergent additive. | a proteolytic strain of bacillus pumilus mp 27 was isolated from water samples of southern ocean produced alkaline protease. since protease production need expensive ingredients, an economically viable process was developed by using low cost carbon source, wheat straw, supplemented with peptone. this protease was active within temperature ranges 10-70°c at ph 9. this process was optimized by response surface methodology using a box bekhman design by design expert 7.0 software that increased the ... | 2016 | 27536284 |
| genome sequence of a multidrug-resistant strain of bacillus pumilus, cb01, isolated from the feces of an american crow, corvus brachyrhynchos. | avian species have the potential to serve as important reservoirs for the spread of pathogenic microorganisms. here, we report the genome sequence of a drug-resistant strain of bacillus pumilus, cb01, isolated from the feces of an american crow, corvus brachyrhynchos. | 2016 | 27540060 |
| bacterial populations associated with smokeless tobacco products. | there are an estimated 8 million users of smokeless tobacco products (stps) in the united states, and yet limited data on microbial populations within these products exist. to better understand the potential microbiological risks associated with stp use, a study was conducted to provide a baseline microbiological profile of stps. a total of 90 samples, representing 15 common stps, were purchased in metropolitan areas in little rock, ar, and washington, dc, in november 2012, march 2013, and july ... | 2016 | 27565615 |
| bacillus pumilus cyanide dihydratase mutants with higher catalytic activity. | cyanide degrading nitrilases are noted for their potential to detoxify industrial wastewater contaminated with cyanide. however, such application would benefit from an improvement to characteristics such as their catalytic activity and stability. following error-prone pcr for random mutagenesis, several cyanide dihydratase mutants from bacillus pumilus were isolated based on improved catalysis. four point mutations, k93r, d172n, a202t, and e327k were characterized and their effects on kinetics, ... | 2016 | 27570524 |
| residue y70 of the nitrilase cyanide dihydratase from bacillus pumilus is critical for formation and activity of the spiral oligomer. | nitrilases pose attractive alternatives to the chemical hydrolysis of nitrile compounds. the activity of bacterial nitrilases towards substrate is intimately tied to the formation of large spiral-shaped oligomers. in the nitrilase cynd (cyanide dihydratase) from bacillus pumilus, mutations in a predicted oligomeric surface region altered its oligomerization and reduced its activity. one mutant, cynd y70c, retained uniform oligomer formation however it was inactive, unlike all other inactive muta ... | 2016 | 27586531 |
| single-site substitutions improve cold activity and increase thermostability of the dehairing alkaline protease (dhap). | to engineer dehairing alkaline protease (dhap) variants to improve cold activity and increase thermostability so these variants are suitable for the leather processing industry. based on previous studies with bacterial alkaline proteases, double-site mutations (w106k/v149i and w106k/m124l) were introduced into the dhap from bacillus pumilus. compared with the wild-type dhap hydrolytic activity, the double-site variant w106k/v149i showed an increase in specific hydrolytic activity at 15 °c by 2.3 ... | 2016 | 27609316 |
| characteristics and complete genome analysis of a novel jumbo phage infecting pathogenic bacillus pumilus causing ginger rhizome rot disease. | tailed phages with genomes larger than 200 kbp are classified as jumbo phage and exhibit extremely high diversity. in this study, a novel jumbo phage, vb_bpum_bpsp, infecting pathogenic bacillus pumilus, the cause of ginger rhizome rot disease, was isolated. notable features of phage vb_bpum_bpsp are the large phage capsid of 137 nm and baseplate-attached curly tail fibers. the genome of the phage is 255,569 bp in size with g+c content of 25.9 %, and it shows low similarity to known biological e ... | 2016 | 27619796 |
| microbial degradation of polyhydroxyalkanoates with different chemical compositions and their biodegradability. | the study addresses degradation of polyhydroxyalkanoates (pha) with different chemical compositions-the polymer of 3-hydroxybutyric acid [p(3hb)] and copolymers of p(3hb) with 3-hydroxyvalerate [p(3hb/3hv)], 4-hydroxybutyrate [p(3hb/4hb)], and 3-hydroxyhexanoate [p(3hb/3hhx)] (10-12 mol%)-in the agro-transformed field soil of the temperate zone. based on their degradation rates at 21 and 28 °c, polymers can be ranked as follows: p(3hb/4hb) > p(3hb/3hhx) > p(3hb/3hv) > p(3hb). the microbial commu ... | 2017 | 27623963 |
| taxonomic identity resolution of highly phylogenetically related strains and selection of phylogenetic markers by using genome-scale methods: the bacillus pumilus group case. | bacillus pumilus group strains have been studied due their agronomic, biotechnological or pharmaceutical potential. classifying strains of this taxonomic group at species level is a challenging procedure since it is composed of seven species that share among them over 99.5% of 16s rrna gene identity. in this study, first, a whole-genome in silico approach was used to accurately demarcate b. pumilus group strains, as a case of highly phylogenetically related taxa, at the species level. in order t ... | 2017 | 27658251 |
| screening and modes of action of antagonistic bacteria to control the fungal pathogen phaeomoniella chlamydospora involved in grapevine trunk diseases. | the antagonistic activity of 46 bacterial strains isolated from bordeaux vineyards were evaluated against phaeomoniella chlamydospora, a major grapevine pathogen involved in esca. the reduction of the necrosis length of stem cuttings ranged between 31.4% and 38.7% for the 8 most efficient strains. two in planta trials allowed the selection of the two best strains, bacillus pumilus (s32) and paenibacillus sp. (s19). their efficacy was not dependent on application method; co-inoculation, preventio ... | 2016 | 27664735 |
| features of gene expression of bacillus pumilus metalloendopeptidase. | features of gene expression of the secreted bacillus pumilus metalloendopeptidase belonging to the adamalysin/reprolysin family were investigated. in the regulatory region of the gene, we identified hypothetical binding sites for transcription factors ccpa and tnra. we found that the expression of the metalloendopeptidase gene is controlled by mechanisms of carbon and nitrogen catabolite repression. in experiments involving nitrogen metabolism regulatory protein mutant strains, we found that the ... | 2016 | 27677556 |
| molecular identification and nanoremediation of microbial contaminants in algal systems using untreated wastewater. | wastewater-algal biomass is a promising option to biofuel production. however, microbial contaminants constitute a substantial barrier to algal biofuel yield. a series of algal strains, nannochloris oculata and chlorella vulgaris samples (n = 30), were purchased from the university of texas, and were used for both stock flask cultures and flat-panel vertical bioreactors. a number of media were used for isolation and differentiation of potential contaminants according to laboratory standards (cls ... | 2016 | 27715467 |
| in-vitro refolding and characterization of recombinant laccase (cota) from bacillus pumilus mk001 and its potential for phenolics degradation. | among lignocellulolytic enzymes, laccases are the most versatile, broadly specific, and largely studied enzyme with a wide range of biotechnological potential. putative laccase (cota) from bacillus pumilus mk001 was cloned and expressed in e. coli. in addition to soluble bioactive fraction, inactive inclusion body fraction was also harvested and refolded under optimized conditions resulting in 64 % of refolding efficiency. the enzyme was found to be thermostable exhibiting a half-life of 60 min ... | 2016 | 27771851 |
| complete genome sequence of bacillus pumilus pdslzg-1, a hydrocarbon-degrading bacterium isolated from oil-contaminated soil in china. | bacillus pumilus strain pdslzg-1, an efficient hydrocarbon-degrading bacterium, was isolated from oil-contaminated soil. here, we present the complete sequence of its circular chromosome and circular plasmid. the genomic information is essential for the study of degradation of oil by b. pumilus pdslzg-1. | 2016 | 27795249 |
| improving the catalytic efficiency of bacillus pumilus cota-laccase by site-directed mutagenesis. | bacterial laccases are potential enzymes for biotechnological applications because of their remarkable advantages, such as broad substrate spectrum, various reactions, high thermostability, wide ph range, and resistance to strongly alkaline environments. however, the use of bacterial laccases for industrialized applications is limited because of their low expression level and catalytic efficiency. in this study, cota, a bacterial laccase from bacillus pumilus, was engineered through presumptive ... | 2017 | 27826721 |
| effects of the dietary supplementation of mixed probiotic spores of bacillus amyloliquefaciens 54a, and bacillus pumilus 47b on growth, innate immunity and stress responses of striped catfish (pangasianodon hypophthalmus). | the study used the mixed probiotics of bacillus amyloliquefaciens 54a and b. pumilus 47b isolated from striped catfish (pangasianodon hypophthalmus) intestine aiming to stimulate growth performance, innate immunity, stress tolerance of striped catfish. the average weight gain (awg), specific growth rate (sgr), and feed conversion ratio (fcr) were analyzed after fish were fed the mixture of probiotics (b. amyloliquefaciens 54a and b. pumilus 47b) at concentrations of 1 × 10(8), 3 × 10(8), and 5 × ... | 2017 | 27836719 |
| a trimeric and thermostable lichenase from b. pumilus us570 strain: biochemical and molecular characterization. | new β-1,3;1,4-glucanase (gluus570) was purified from a newly isolated bacillus pumilus us570 strain. the enzyme was active in a wide range of ph and temperature and displayed a great thermostability with a half-life of 30min at 80°c. the enzyme was demonstrated to be a lichenase since it was only active toward glucan containing β-1,3;1,4- linkages. the analysis of the enzyme in native and denaturing conditions suggests that it has a trimeric form (75kda). this is the first report on the purifica ... | 2017 | 27838418 |
| influence of endophytic bacillus pumilus and edta on the phytoextraction of cu from soil by using cicer arietinum. | in developing countries, soil contamination with metals is ubiquitous, which poses a serious threat to the ecosystem. the current study was designed to screen out the nested belongings of cicer arietinum plants and bacillus pumilus (kf 875447) in extracting copper (cu) from contaminated soils. a pot experiment was executed by growing c. arietinum seedlings either inoculated with b. pumilus or uninoculated along with the application of 5 mm ethylenediaminetetraacetic acid (edta). plants were subj ... | 2017 | 27869502 |
| evaluating uv-c led disinfection performance and investigating potential dual-wavelength synergy. | a dual-wavelength uv-c led unit, emitting at peaks of 260 nm, 280 nm, and the combination of 260|280 nm together was evaluated for its inactivation efficacy and energy efficiency at disinfecting escherichia coli, ms2 coliphage, human adenovirus type 2 (hadv2), and bacillus pumilus spores, compared to conventional low-pressure and medium-pressure uv mercury vapor lamps. the dual-wavelength unit was also used to measure potential synergistic effects of multiple wavelengths on bacterial and viral i ... | 2017 | 27889622 |
| the impact of silver nanoparticles produced by bacillus pumilus as antimicrobial and nematicide. | this study evaluates the potential application of silver nanoparticles (agnps) as antimicrobial or nematicidal agents produced by the extremophile bacillus pumilus, which was isolated from the alkaline wadi el-natrun lake in egypt. the agnps were characterized by ultraviolet-visible absorption spectroscopy, transmission electron microscopy, and energy dispersive x-ray spectroscopy. the size of agnps formed ranged from 20.12 to 29.48 nm. panagrellus redivivus was exposed to different concentratio ... | 2016 | 27891113 |
| high-level expression of improved thermo-stable alkaline xylanase variant in pichia pastoris through codon optimization, multiple gene insertion and high-density fermentation. | in paper industry, xylanases are used to increase the pulp properties in bleaching process as its eco-friendly nature. the xylanases activity is hindered by high temperature and alkaline conditions with high enzyme production cost in the paper industry. here, xynhb, an alkaline stable xylanase from bacillus pumilus hbp8 was mutated at n188a to xynhbn188a. expressed mutant in e. coli showed 1.5-fold higher xylanase activity than xynhb at 60 °c. the mutant expressed in pichia pastoris was glycosyl ... | 2016 | 27897254 |
| a report on extensive lateral genetic reciprocation between arsenic resistant bacillus subtilis and bacillus pumilus strains analyzed using rapd-pcr. | the study involves isolation of arsenic resistant bacteria from soil samples. the characterization of bacteria isolates was based on 16s rrna gene sequences. the phylogenetic consanguinity among isolates was studied employing rpob and gltx gene sequence. rapd-pcr technique was used to analyze genetic similarity between arsenic resistant isolates. in accordance with the results bacillus subtilis and bacillus pumilus strains may exhibit extensive horizontal gene transfer. arsenic resistant potency ... | 2017 | 27956257 |
| pathogenic features of heterotrophic plate count bacteria from drinking-water boreholes. | evidence suggests that heterotrophic plate count (hpc) bacteria may be hazardous to humans with weakened health. we investigated the pathogenic potential of hpc bacteria from untreated borehole water, consumed by humans, for: their haemolytic properties, the production of extracellular enzymes such as dnase, proteinase, lipase, lecithinase, hyaluronidase and chondroitinase, the effect simulated gastric fluid has on their survival, as well as the bacteria's antibiotic-susceptible profile. hutu-80 ... | 2016 | 27959868 |
| dynamics of bacterial class bacilli in the deepest valley lake of kashmir-the manasbal lake. | in recognition of the importance of bacteria as ecological indicators of the aquatic systems a comprehensive and systematic analysis was carried out on manasbal lake, the deepest spring fed valley lake of kashmir. the main objective envisaged was to analyze bacterial community composition (bcc) and for this purpose systematic and regular sampling of waters from ten different sampling stations, predetermined in the lake according to differences in degree of human interference and also as zones of ... | 2017 | 28087491 |
| expression and secretion of cyan fluorescent protein (cfp) in b. subtilis using the chitinase promoter from bacillus pumilus sg2 | improved cyan fluorescent protein (icfp) is a monochromic, green fluorescent protein (gfp) derivative produced by aequorea macrodactyla in a process similar to gfp. this protein has strong absorption spectra at wavelengths 426-446 nm. icfp can be used in cell, organelle or intracellular protein labeling, investigating the protein-protein interactions as well as assessing the promoter activities. | 2017 | 28088132 |
| a functional endonuclease q exists in the bacterial domain: identification and characterization of endonuclease q from bacillus pumilus. | dna base deamination occurs spontaneously under physiological conditions and is promoted by high temperature. therefore, hyperthermophiles are expected to have efficient repair systems of the deaminated bases in their genomes. endonuclease q (endoq) was originally identified from the hyperthermophlic archaeon, pyrococcus furiosus, as a hypoxanthine-specific endonuclease recently. further biochemical analyses revealed that endoq also recognizes uracil, xanthine, and the ap site in dna, and is pro ... | 2017 | 28095753 |
| the role of metals in the reaction catalyzed by metal-ion-independent bacillary rnase. | extracellular enzymes of intestinal microbiota are the key agents that affect functional activity of the body as they directly interact with epithelial and immune cells. several species of the bacillus genus, like bacillus pumilus, a common producer of extracellular rnase binase, can populate the intestinal microbiome as a colonizing organism. without involving metal ions as cofactors, binase depolymerizes rna by cleaving the 3',5'-phosphodiester bond and generates 2',3'-cyclic guanosine phospha ... | 2016 | 28096759 |
| mechanism of anti-vibrio activity of marine probiotic strain bacillus pumilus h2, and characterization of the active substance. | vibriosis is a major epizootic disease that impacts free-living and farmed fish species worldwide. use of probiotics is a promising approach for prevention of vibrio infections in aquaculture. a probiotic anti-vibrio strain, bacillus pumilus h2, was characterized, and the mechanism of its effect was investigated. all 29 vibrio strains tested were growth-inhibited by h2. the anti-vibrio substance present in cell-free supernatant of h2 was purified and characterized by reversed-phase hplc. minimum ... | 2017 | 28097594 |
| enhanced performance of the microalga chlorella sorokiniana remotely induced by the plant growth-promoting bacteria azospirillum brasilense and bacillus pumilus. | remote effects (occurring without physical contact) of two plant growth-promoting bacteria (pgpb) azospirillum brasilense cd and bacilus pumilus es4 on growth of the green microalga chlorella sorokiniana utex 2714 were studied. the two pgpb remotely enhanced the growth of the microalga, up to six-fold, and its cell volume by about three-fold. in addition to phenotypic changes, both bacteria remotely induced increases in the amounts of total lipids, total carbohydrates, and chlorophyll a in the c ... | 2017 | 28145473 |
| green synthesis of rifampicin-loaded copper nanoparticles with enhanced antimicrobial activity. | the antimicrobial properties of copper and rifampicin-loaded copper nanoparticles were investigated using four strains: staphylococcus aureus, escherichia coli, bacillus pumilis and pseudomonas fluorescens. spherical-shaped copper nanoparticles were synthesized via green reduction method from the peppermint extract. it was found that adsorption of rifampicin on the copper nanosurface enhances its biological activity and prevents the development of resistance. the interactions between rifampicin- ... | 2017 | 28150115 |
| antimicrobial effects of novel peptides cot2 and sot2 derived from crocodylus siamensis and pelodiscus sinensis ovotransferrins. | in light of the increasing threat of bacterial drug resistance to human health on a global scale, research and development of antimicrobial peptides as a novel class of potent antibiotics has gained considerable attention. the present study focuses on the structural evaluation and membrane interaction of two new cationic antimicrobial peptides, cot2 and sot2, derived from siamese crocodile (crocodylus siamensis) and chinese softshell turtle (pelodiscus sinensis) ovotransferrins. here, cot1 (+3) ... | 2017 | 28159460 |
| microorganisms and heavy metals associated with atmospheric deposition in a congested urban environment of a developing country: sri lanka. | the presence of bacteria and heavy metals in atmospheric deposition were investigated in kandy, sri lanka, which is a typical city in the developing world with significant traffic congestion. atmospheric deposition samples were analyzed for al, cr, mn, fe, ni, cu, zn, cd and pb which are heavy metals common to urban environments. al and fe were found in high concentrations due to the presence of natural sources, but may also be re-suspended by vehicular traffic. relatively high concentrations of ... | 2017 | 28185730 |
| insight into the bacterial diversity of fermentation woad dye vats as revealed by pcr-dgge and pyrosequencing. | the bacterial diversity in fermenting dye vats with woad (isatis tinctoria l.) prepared and maintained in a functional state for approximately 12 months was examined using a combination of culture-dependent and -independent pcr-dgge analyses and next-generation sequencing of 16s rrna amplicons. an extremely complex ecosystem including taxa potentially contributing to both indigo reduction and formation, as well as indigo degradation was found. pcr-dgge analyses revealed the presence of paenibaci ... | 2017 | 28246965 |
| [exogenous bacillus pumilus rnase (binase) suppresses the reproduction of reovirus serotype 1]. | the experimental study identified the antiviral activity of bacillus pumilus rnase (binase) against the reovirus of serotype 1/strain lang. for the first time, it has been found that 50 μg/ml of binase effectively reduced the hemagglutinin and cytocidal activity of reovirus in vero cell line. the preincubation of the enzyme with reovirus before infection of the cells inhibited the viral replication. to determine the stagedependent effect of reovirus reproduction upon binase inhibition, the infec ... | 2017 | 28251973 |
| regulatory characteristics of bacillus pumilus protease promoters. | expression of extracellular protease genes of bacilli is subject to regulation by many positive and negative regulators. here we analyzed 5' regulatory regions of genes encoding proteolytic proteases aprbp, gsebp, and mprbp from bacillus pumilus strain 3-19. gfp fusion constructs with upstream genomic regions of different lengths were created for all three genes to identify their natural promoters (regulatory regions). our results suggest that the aprbp gene, encoding the major subtilisin-like p ... | 2017 | 28258295 |
| chemical composition and biological properties of satureja avromanica maroofi. | satureja avromanica is an indigenous plant which is frequently used as a spice in avraman-kurdistan region of iran. the present study aimed to investigate the chemical composition, antimicrobial and antioxidant properties of the s. avromanica. in addition, rosmarinic acid and total phenolic content of s. avromanica was assessed by spectrophotometric method and hptlc. the essential oil and methanolic extract were isolated by hydrodistillation and maceration methods, respectively. a total of 32 co ... | 2017 | 28298686 |
| stratosphere conditions inactivate bacterial endospores from a mars spacecraft assembly facility. | every spacecraft sent to mars is allowed to land viable microbial bioburden, including hardy endospore-forming bacteria resistant to environmental extremes. earth's stratosphere is severely cold, dry, irradiated, and oligotrophic; it can be used as a stand-in location for predicting how stowaway microbes might respond to the martian surface. we launched e-mist, a high-altitude nasa balloon payload on 10 october 2015 carrying known quantities of viable bacillus pumilus safr-032 (4.07 × 10(7) spor ... | 2017 | 28323456 |
| characterization of root-associated bacteria from paddy and its growth-promotion efficacy. | bacteria from rhizosphere (bacillus pumilus) and endorhizophere (pseudomonas pseudoalcaligenes) of rice plant were isolated and evaluated for their effect on the growth-promotion efficiency on rice in greenhouse. ability to solubilize phosphate, siderophore, indoleacetic acid (iaa), gibberellin production and utilization of acc (1-aminocyclopropane-1-carboxylate) as sole nitrogen source were evaluated, which were produced in high concentration by p. pseudoalcaligenes in this present study. inocu ... | 2014 | 28324437 |
| isolation and cloning of the endoglucanase gene from bacillus pumilus and its expression in deinococcus radiodurans. | with the aim of engineering a strain of bacteria that could be used for bioremediation of cellulosic waste in radioactive environments, the gene for the secreted endoglucanase enzyme of bacillus pumilis was decided to be cloned into the radiotolerant bacterium, deinococcus radiodurans. the endoglucanase gene from b. pumilus was pcr amplified and cloned into escherichiacoli dh5α using a pdrive vector. it was subsequently sub-cloned into e.coli-deinococcus shuttle vector prad1 downstream of the de ... | 2014 | 28324463 |
| modulation of xylanase production from alkaliphilic bacillus pumilus vlk-1 through process optimization and temperature shift operation. | this study was aimed at enhancing the production of xylanase from an alkaliphilic bacillus pumilus vlk-1 in submerged fermentation using wheat bran, a cheap and abundantly available agro-residue, through process optimization and to monitor the effect of temperature shift operation on it. the potential of xylanase in saccharification of wheat straw was also investigated. the results showed that optimization of the fermentation process by one variable approach increased the enzyme yield from 402 t ... | 2014 | 28324471 |