| draft whole genome sequence of bacillus pumilus strain 3-19, a chemical mutant overproducing extracellular ribonuclease. | here, we present a draft genome sequence of bacillus pumilus strain 3-19. it was derived from soil-isolated b. pumilus 7p using chemical mutagenesis and is characterized by elevated production of extracellular ribonuclease which is known to possess different biological activities with potential of applications in experimental research, medicine, and biotechnology. | 2014 | 25059870 |
| characterization of industrially-valuable xylano-pectinolytic enzymes produced concurrently by a novel isolate of bacillus pumilus. | xylanase and polygalacturonase were concurrently produced by a novel alkalo-thermotolerant bacillus pumilus ajk. they were purified and characterized to evaluate their potential for various industrial applications. xylanase was purified to 19-fold with 67 % recovery and polygalacturonase up to 23-fold with 75 % recovery. existence of multiple forms of xylanase was indicated by its elution-profile through sephadex g-100 as two peaks, xylanase-i and xylanase-ii, with molecular weights of ~24.5 and ... | 2014 | 25048224 |
| new derivatives of nonactic and homononactic acids from bacillus pumilus derived from breynia fruticosa. | six new nonactic and homononactic acid derivatives, ethyl homononactate (1), ethyl nonactate (2), homononactyl homononactate (6), ethyl homononactyl nonactate (7), ethyl homononactyl homononactate (8), and ethyl nonactyl nonactate (9), as well as four known compounds, homononactic acid (3), nonactic acid (4), homononactyl nonactate (5), and bishomononactic acid (10), were isolated from culture broth of bacillus pumilus derived from breynia fruticosa. the structures of new compounds were elucidat ... | 2014 | 25044594 |
| expression of concern: bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress. | | 2014 | 25010116 |
| [screening and identification of bacillus pumilus producing double active protein of anticoagulation and thrombolysis]. | the aim of this study was to screen bacteria that can produce antithrombotic. | 2014 | 24984527 |
| identification and characterization of the sudanese bacillus thuringiensis and related bacterial strains for their efficacy against helicoverpa armigera and tribolium castaneum. | forty-four isolates of bacillus thuringiensis like bacteria from various sources in different locations from sudan were tested for their insecticidal activity. the toxicity of these isolates ranged from 6.6 to 70% to the neonates of cotton bollworm, helicoverpa armigera at 10 ppm concentration. the most effective ones are kb-29, st-6 and wh-1 comparable with hd-1. toxicity of isolates to larvae of the red flour beetle, tribolium castaneum ranged from 20 to 100%. isolates st-2 and st-23 gave 100% ... | 2014 | 24956895 |
| draft genome sequence of bacillus pumilus 7p, isolated from the soil of the tatarstan republic, russia. | here, we present a draft genome sequence of bacillus pumilus strain 7p. this strain was isolated from soil as an extracellular rnase-producing microorganism. the rnase of b. pumilus 7p is considered to be a potential antiviral and therapeutic antitumor agent, and it might be appropriate for agriculture and academic synthesis of oligoribonucleotides. | 2014 | 24926064 |
| exo-pectinase production by bacillus pumilus using different agricultural wastes and optimizing of medium components using response surface methodology. | in this research, the production of exo-pectinase by bacillus pumilus using different agricultural wastes was studied. agricultural wastes containing pectin such as wheat bran, sugar beet pulp, sunflower plate, orange peel, banana peel, apple pomace and grape pomace were tested as substrates, and activity of exo-pectinase was determined only in the mediums containing sugar beet pulp and wheat bran. then, effects of parameters such as concentrations of solid substrate (wheat bran and sugar beet p ... | 2014 | 24819433 |
| molecular characterization of cellulose-degrading bacillus pumilus from the soil of tea garden, darjeeling hills, india. | bio-fuel produced from ethanol is economically and environmentally advantageous in context of changing global climate. a large number of microorganisms are capable of cellulase production but most of them cannot be utilized commercially due to their low activity. in the present study, an effiecient cellulose degrading strain of bacillus pumilus was obtained after thorough screening for the production of extracellular cellulases. out of a total of 144 microbes isolated from soils of darjeeling hi ... | 2014 | 24813013 |
| iterative genome correction largely improves proteomic analysis of nonmodel organisms. | the current application and development of proteomic studies typically depend on the availability of sequenced genomes. protein identification based on the detected peptides with liquid chromatography tandem mass spectrometry is limited by the absence of sequenced genomes in many nonmodel organisms. in this study, we demonstrated a new strategy based on our stable, accurate, and error-tolerant fanse (fast and accurate mapping tool for nucleotide sequencing datasets) mapping algorithm to correct ... | 2014 | 24809469 |
| in vitro assessment of marine bacillus for use as livestock probiotics. | six antimicrobial-producing seaweed-derived bacillus strains were evaluated in vitro as animal probiotics, in comparison to two bacillus from an eu-authorized animal probiotic product. antimicrobial activity was demonstrated on solid media against porcine salmonella and e. coli. the marine isolates were most active against the latter, had better activity than the commercial probiotics and bacillus pumilus wit 588 also reduced e. coli counts in broth. all of the marine bacillus tolerated physiolo ... | 2014 | 24796302 |
| enhanced degradation of textile effluent in constructed wetland system using typha domingensis and textile effluent-degrading endophytic bacteria. | textile effluent is one of the main contributors of water pollution and it adversely affects fauna and flora. constructed wetland is a promising approach to remediate the industrial effluent. the detoxification of industrial effluent in a constructed wetland system may be enhanced by applying beneficial bacteria that are able to degrade contaminants present in industrial effluent. the aim of this study was to evaluate the influence of inoculation of textile effluent-degrading endophytic bacteria ... | 2014 | 24755300 |
| genome sequence of bacillus pumilus mtcc b6033. | bacillus pumilus is a gram-positive, rod-shaped, aerobic bacterium isolated from the soil. b. pumilus strain b6033 was originally selected as a biocatalyst for the stereospecific oxidation of β-lactams. here, we present a 3.8-mb assembly of its genome, which is the second fully assembled genome of a b. pumilus strain. | 2014 | 24744340 |
| draft genome sequence of bacillus pumilus fairview, an isolate recovered from a microbial methanogenic enrichment of coal seam gas formation water from queensland, australia. | despite its global abundance, bacillus pumilus is poorly studied. the fairview strain was obtained from a methanogenic anaerobic coal digester. the draft genome sequence was 3.8 mbp long and contained 3,890 protein-coding genes. like the safr-032 strain, it includes b. pumilus-specific proteins that likely confer enhanced resistance to environmental stresses. | 2014 | 24744330 |
| the human immune system recognizes neopeptides derived from mitochondrial dna deletions. | mutations in mitochondrial (mt) dna accumulate with age and can result in the generation of neopeptides. immune surveillance of such neopeptides may allow suboptimal mitochondria to be eliminated, thereby avoiding mt-related diseases, but may also contribute to autoimmunity in susceptible individuals. to date, the direct recognition of neo-mtpeptides by the adaptive immune system has not been demonstrated. in this study we used bioinformatics approaches to predict mhc binding of neopeptides iden ... | 2014 | 24733843 |
| an alkali-thermostable xylanase from bacillus pumilus functionally expressed in kluyveromyces lactis and evaluation of its deinking efficiency. | this work aimed at studying the recombinant expression of an alkali- and thermo-stable xylanase from bacillus pumilus in kluyveromyces lactis and its use in deinking of civic paper waste. efficient expression with a 3-fold increase in the activity than the native organism was achieved. an inducer concentration of 2.5% and medium ph of 9.0 was the best for enzyme expression. purified enzyme showed an optimum activity at temperatures 50 and 60°c and ph 9.0 and 10.0, respectively. at ph 12.0, enzym ... | 2014 | 24709528 |
| bacillus pumilus s124a carboxymethyl cellulase; a thermo stable enzyme with a wide substrate spectrum utility. | bacillus pumilus s124a was identified as carboxymethyl cellulase producing bacteria from azorean bacillus collection (lab collection), which was isolated in local soils. the bacterium was identified by 16s rrna sequence and designated as bacillus pumilus s124a. ncbi-blast analysis showed b. pumilus s124a; 16s rrna sequence has high identity to other b. pumilus strains. phylogenetic analysis showed b. pumilus s124a close to b. pumilus lzbp14 strain. cmcellulase was purified from cells-free supern ... | 2014 | 24657377 |
| exploitation of starch industry liquid by-product to produce bioactive peptides from rice hydrolyzed proteins. | small peptides show higher antioxidant capacity than native proteins and may be absorbed in the intestine without further digestion. in our study, a protein by-product from rice starch industry was hydrolyzed with commercial proteolytic enzymes (alcalase, neutrase, flavourzyme) and microbial whole cells of bacillus spp. and the released peptides were tested for antioxidant activity. among enzymes, alcalase was the most performing, while microbial proteolytic activity was less efficient. converse ... | 2014 | 24594175 |
| evaluation of the efficacy and safety of a marine-derived bacillus strain for use as an in-feed probiotic for newly weaned pigs. | forty eight individual pigs (8.7±0.26 kg) weaned at 28±1 d of age were used in a 22-d study to evaluate the effect of oral administration of a bacillus pumilus spore suspension on growth performance and health indicators. treatments (n = 16) were: (1) non-medicated diet; (2) medicated diet with apramycin (200 mg/kg) and pharmacological levels of zinc oxide (2,500 mg zinc/kg) and (3) b. pumilus diet (non-medicated diet + 10(10) spores/day b. pumilus). final body weight and average daily gain tend ... | 2014 | 24586349 |
| characterization and a point mutational approach of a psychrophilic lipase from an arctic bacterium, bacillus pumilus. | a bacterium with lipolytic activity was isolated from the chukchi sea within the arctic ocean. the lipase bpl5 from the isolate, bacillus pumilus arcl5, belongs to subfamily 4 of lipase family i. the optimum ph and temperature of the recombinant enzyme bpl5, as expressed in escherichia coli, were 9.0 and 20 °c, respectively. the enzyme retained 85 % of its activity at 5 °c. there was a significant difference between temperatures for maximal activity (20 °c) and for protein denaturation (approx. ... | 2014 | 24563306 |
| the discovery of phiagate, a novel phage infecting bacillus pumilus, leads to new insights into the phylogeny of the subfamily spounavirinae. | the bacillus phage phiagate is a novel myovirus isolated from the waters of lake góreckie (a eutrophic lake in western poland). the bacteriophage infects bacillus pumilus, a bacterium commonly observed in the mentioned reservoir. analysis of the phiagate genome (149844 base pairs) resulted in 204 predicted protein-coding sequences (cdss), of which 53 could be functionally annotated. further investigation revealed that the bacteriophage is a member of a previously undescribed cluster of phages (f ... | 2014 | 24466180 |
| bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress. | bacillus pumilus is characterized by a higher oxidative stress resistance than other comparable industrially relevant bacilli such as b. subtilis or b. licheniformis. in this study the response of b. pumilus to oxidative stress was investigated during a treatment with high concentrations of hydrogen peroxide at the proteome, transcriptome and metabolome level. genes/proteins belonging to regulons, which are known to have important functions in the oxidative stress response of other organisms, we ... | 2014 | 24465625 |
| 2,5-diketopiperazines produced by bacillus pumilus during bacteriolysis of arthrobacter citreus. | we report the detection by gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry analyses of the secreted 2,5-diketopiperazines (dkps) cyclo(-ala-pro), cyclo(-gly-pro), cyclo(-val-pro), cyclo(-ile-pro), cyclo(-leu-pro), cyclo(-pro-pro), cyclo(-hyp-pro), cyclo(-met-pro), and cyclo(-phe-pro) produced by bacillus pumilus. the study focuses on a marine isolate and a laboratory test strain of b. pumilus with capabilities to lyse pregrown living cell lawns of different bacte ... | 2014 | 24449388 |
| modulation of xylanase production from alkaliphilic bacillus pumilus vlk-1 through process optimization and temperature shift operation. | this study was aimed at enhancing the production of xylanase from an alkaliphilic bacillus pumilus vlk-1 in submerged fermentation using wheat bran, a cheap and abundantly available agro-residue, through process optimization and to monitor the effect of temperature shift operation on it. the potential of xylanase in saccharification of wheat straw was also investigated. the results showed that optimization of the fermentation process by one variable approach increased the enzyme yield from 402 t ... | 2014 | 28324471 |
| isolation and cloning of the endoglucanase gene from bacillus pumilus and its expression in deinococcus radiodurans. | with the aim of engineering a strain of bacteria that could be used for bioremediation of cellulosic waste in radioactive environments, the gene for the secreted endoglucanase enzyme of bacillus pumilis was decided to be cloned into the radiotolerant bacterium, deinococcus radiodurans. the endoglucanase gene from b. pumilus was pcr amplified and cloned into escherichiacoli dh5α using a pdrive vector. it was subsequently sub-cloned into e.coli-deinococcus shuttle vector prad1 downstream of the de ... | 2014 | 28324463 |
| characterization of root-associated bacteria from paddy and its growth-promotion efficacy. | bacteria from rhizosphere (bacillus pumilus) and endorhizophere (pseudomonas pseudoalcaligenes) of rice plant were isolated and evaluated for their effect on the growth-promotion efficiency on rice in greenhouse. ability to solubilize phosphate, siderophore, indoleacetic acid (iaa), gibberellin production and utilization of acc (1-aminocyclopropane-1-carboxylate) as sole nitrogen source were evaluated, which were produced in high concentration by p. pseudoalcaligenes in this present study. inocu ... | 2014 | 28324437 |
| physical impaction injury effects on bacterial cells during spread plating influenced by cell characteristics of the organisms. | to understand the factors that contribute to the variations in colony-forming units (cfu) in different bacteria during spread plating. | 2014 | 24314107 |
| complete genome of bacillus pumilus siphophage riggi. | bacillus pumilus is primarily used in the agricultural industry to promote plant growth and provide resistance to bacterial and fungal plant diseases. it has recently, however, been shown to cause disease in humans. here, we announce the complete genome of b. pumilus phage riggi. | 2013 | 24309728 |
| complete genome of bacillus pumilus siphophage glittering. | bacillus pumilus is a gram-positive bacterium widely used in agriculture both as an antifungal and as a growth-promoting symbiont. b. pumilus is rarely infectious but has recently been shown to infect humans. here, we present the complete genome of b. pumilus phage glittering, a potential biocontrol agent for b. pumilus. | 2013 | 24309725 |
| complete genome of bacillus pumilus siphophage blastoid. | phage blastoid is a siphophage that infects bacillus pumilus. b. pumilus is widely used in agriculture but has recently been linked to cases of food poisoning. here, we report the complete genome of blastoid and discuss unique genomic characteristics. | 2013 | 24309724 |
| lipase production from a novel thermo-tolerant and extreme acidophile bacillus pumilus using palm oil as the substrate and treatment of palm oil-containing wastewater. | the thermo-tolerant and extreme acidophilic microorganism bacillus pumilus was isolated from the soil collected from a commercial edible-oil extraction industry. optimisation of conditions for the lipase production was conducted using response surface methodology. the optimum conditions for obtaining the maximum activity (1,100 u/ml) of extremely acidic thermostable lipase were fermentation time, 96 h; ph, 1; temperature, 50 °c; and concentration of palm oil, 50 g/l. after purification, a 7.1-fo ... | 2014 | 24293300 |
| characterization of cis-acting elements residing in the chitinase promoter of bacillus pumilus sg2. | bacillus pumilus sg2 is a chitinolytic bacterium that produces two chitinases, namely chis and chil. the chis and chil genes are consecutively expressed under a common promoter. regulation of the chis and chil genes is under the control of carbon catabolite repression (ccr) in b. pumilus. this study aimed to investigate the cis-acting elements of the chitinase promoter. for this purpose, we transferred the chis gene along with its specific promoter to bacillus subtilis as a host. primer extensio ... | 2014 | 24293243 |
| phylogenetic diversity of the bacillus pumilus group and the marine ecotype revealed by multilocus sequence analysis. | bacteria closely related to bacillus pumilus cannot be distinguished from such other species as b. safensis, b. stratosphericus, b. altitudinis and b. aerophilus simply by 16s rrna gene sequence. in this report, 76 marine strains were subjected to phylogenetic analysis based on 7 housekeeping genes to understand the phylogeny and biogeography in comparison with other origins. a phylogenetic tree based on the 7 housekeeping genes concatenated in the order of gyrb-rpob-pyca-pyre-mutl-aroe-trpb was ... | 2013 | 24244618 |
| biodegradation of low-density polyethylene by marine bacteria from pelagic waters, arabian sea, india. | sixty marine bacteria isolated from pelagic waters were screened for their ability to degrade low-density polyethylene; among them, three were positive and able to grow in a medium containing polythene as the sole carbon source. the positive isolates were identified as kocuria palustris m16, bacillus pumilus m27 and bacillus subtilis h1584 based on the 16s rrna gene sequence homology. the weight loss of polyethylene was 1%, 1.5% and 1.75% after 30 days of incubation with the m16, m27 and h1584 i ... | 2013 | 24210946 |
| novel extremely acidic lipases produced from bacillus species using oil substrates. | the extremely acidophilic microorganisms bacillus pumilus and bacillus subtilis were isolated from soil collected from the commercial edible oil and fish oil extraction industry. optimization of conditions for acidic lipase production from b. pumilus and b. subtilis using palm oil and fish oil, respectively, was carried out using response surface methodology. the extremely acidic lipases, thermo-tolerant acidic lipase (tal) and acidic lipase (al), were produced by b. pumilus and b. subtilis, res ... | 2014 | 24185617 |
| biological and genomic analysis of a pbsx-like defective phage induced from bacillus pumilus ab94180. | defective prophages, which are found in the genomes of many bacteria, are unable to complete a viral replication cycle and propagate in their hosts as healthy prophages. they package random dna fragments derived from various sites of the host chromosome instead of their own genomes. in this study, we characterized a defective phage, pbp180, which was induced from bacillus pumilus ab94180 by treatment with mitomycin c. electron microscopy showed that the pbp180 particle has a head with a hexagona ... | 2014 | 24154951 |
| production and characterization of alkaline protease from hemoglobin-degrading bacillus pumilus njm4 to produce fermented blood meal. | the aim of the research was to isolate the hemoglobin-degrading bacterial strain to produce fermented blood meal and to characterize the protease produced by this strain. the strain njm4, a kind of hemoglobin-degrading bacterial strain, was isolated by blood agar plates from slaughterhouse and identified as a bacillus pumilus by physiological, biochemical, and morphological characteristics and by 16s rrna gene sequencing. bacillus pumilus njm4 could degrade hemoglobin up to 85% in 36 h under the ... | 2012 | 24082276 |
| draft genome sequence of bacillus pumilus ccma-560, isolated from an oil-contaminated mangrove swamp. | bacillus pumilus strain ccma-560 was isolated from an oil-contaminated mangrove swamp and was shown to produce biosurfactants. the strain appears to be capable of degrading some plant cell wall-related compounds, including hemicelluose and pectin. genes for biopolymer export and polysaccharide intercellular adhesin synthesis were also annotated. | 2013 | 24029758 |
| studies on degradation efficiency of polycaprolactone by a naturally-occurring bacterium. | we report a strain of bacillus, isolated from the rhizosphere of the mangrove sesuvium portulacastrum, that degrades polycaprolactone (pcl) on timescales that are a factor of three shorter than hitherto reported, with complete degradation in only 20 days. the bacterium has been identified as bacillus pumilus by means of 16s rrna gene sequencing and fame analysis; it secretes proteases and lipases and its 'de-polymerase' activity is evident by the zone of clearing in emulsified pcl. it is an aero ... | 2013 | 24006806 |
| a possible mechanism of action of plant growth-promoting rhizobacteria (pgpr) strain bacillus pumilus wp8 via regulation of soil bacterial community structure. | according to the traditional view, establishment and maintenance of critical population densities in the rhizosphere was the premise of pgpr to exert growth-promoting effects. in light of the facts that soil bacterial community structures can be changed by some pgpr strains including bacillus pumilus wp8, we hypothesize that regulation of soil bacterial community structure is one of the plant growth-promoting mechanisms of b. pumilus wp8, rather than depending on high-density cells in soil. in t ... | 2013 | 24005176 |
| [structural properties and functional importance of metzincin metalloproteinases]. | here wediscuss known properties of metzincin metalloproteinases, their structure, physiological roles in the cell and potential medical uses. we also present results describing a novel extracellular metzincin metalloproteinase from bacillus pumilus with a unique combination of properties typical for both astacins and adamalysins. | 2015 | 25702412 |
| novel method utilizing microbial treatment for cleaner production of diosgenin from dioscorea zingiberensis c.h. wright (dzw). | a novel method utilizing microbial treatment for cleaner production of diosgenin from dioscorea zingiberensis c.h. wright (dzw) was presented. a new bacillus pumilus hr19, which has the great ability to secrete pectinase, was screened and applied in the microbial treatment. low-pressure steam expansion pretreatment (lsep) was employed in advance to assist microbial treatment efficiently in releasing saponins, which are the precursors of diosgenin. compared with the traditional process of acid hy ... | 2013 | 23973974 |
| an assay for functional xylose transporters in saccharomyces cerevisiae. | it has been considered that more efficient uptake of xylose could promote increased xylose metabolic capacity of several microorganisms. in this study, an assay to screen xylose transporters was established in the saccharomyces cerevisiae strain, which expresses the xylosidase gene of bacillus pumilus intracellularly. the absorbed xylose analog p-nitrophenyl-β-d-xylopyranoside (pnpx) rapidly hydrolyzed to p-nitrophenol (pnp), which displayed a yellow tint when exposed to xylosidase in vivo. the ... | 2013 | 23928049 |
| reducing the radiation sterilization dose improves mechanical and biological quality while retaining sterility assurance levels of bone allografts. | bone allografts carry a risk of infection, so terminal sterilization by gamma irradiation at 25kgy is recommended; but is deleterious to bone quality. contemporary bone banking significantly reduces initial allograft bioburden, questioning the need to sterilize at 25kgy. | 2013 | 23912050 |
| genomic characterization of six novel bacillus pumilus bacteriophages. | twenty-eight bacteriophages infecting the local host bacillus pumilus bl-8 were isolated, purified, and characterized. nine genomes were sequenced, of which six were annotated and are the first of this host submitted to the public record. the 28 phages were divided into two groups by sequence and morphological similarity, yielding 27 cluster bpa phages and 1 cluster bpb phage, which is a bl-8 prophage. most of the bpa phages have a host range restricted to distantly related strains, b. pumilus a ... | 2013 | 23906709 |
| improving the chitinolytic activity of bacillus pumilus sg2 by random mutagenesis. | bacillus pumilus sg2, a halotolerant strain, expresses two major chitinases designated chis and chil that were induced by chitin and secreted into the supernatant. the present work aimed to obtain a mutant with higher chitinolytic activity through mutagenesis of bacillus pumilus sg2 using a combination of uv irradiation and nitrous acid treatment. following mutagenesis and screening on chitin agar and subsequent formation of halos, the mutated strains were examined for degradation of chitin unde ... | 2013 | 23867702 |
| bioinspired inimitable cadmium telluride quantum dots for bioimaging purposes. | synthesis of quantum nanoparticles of specific size, shape and composition are an aspect important in nanotechnology research. although these nanostructures are routinely synthesized by chemical routes, the use of microorganisms has emerged as a promising option. the synthesis of cadmium telluride (cdte) quantum dots by two hitherto unreported marine bacteria (bacillus pumilus and serratia marcescens) is reported here. ultraviolet-visible (uv-vis) spectroscopy indicated the synthesis of cdte nan ... | 2013 | 23862414 |
| [properties of bacillus pumilus subtilisin like proteinase secreted from recombinant strain on different growth stages]. | bacillus pumilus 3-19 glutamylendopeptidase has been isolated from culture liquid of bacillus subtilis recombinant strain on different growth stages: growth retardation (early enzyme) and stationary phase (late enzyme). the effect of purified proteinase of different growth stages on insulin beta-chain, protein and oligopeptide substrates has been studied. comparative study of physicochemical properties of early and late proteinases was carried out. two protein fractions were different in catalyt ... | 2013 | 23844506 |
| synthesis and biological evaluation of bis-imidazolidineiminothiones: a comparative study. | a series of 15 novel symmetrical and non-symmetrical bis-imidazolidineiminothiones (6a-g, 7a-e, 8a,b, and 9) with various substituents at n-(1) (p-tolyl, p-methoxyphenyl, p-ethoxyphenyl, p-chlorophenyl, p-bromophenyl, p-iodophenyl, and 3,5-dichlorophenyl) and different linkers between the n-(3) atoms [4,4'-oxybis(4,1-phenylene), 2,2'-dimethoxybiphenyl, and (1,3,3-trimethylcyclohexyl)methyl)] were prepared in 65-75% yields from substituted n-arylcyanothioformanilides and various bis-isocyanates. ... | 2013 | 23836516 |
| optimization of chitin extraction from shrimp waste with bacillus pumilus a1 using response surface methodology. | chitin extraction from shrimp shells by biological treatment, using the bacilli bacillus pumilus a1, is a non-polluting method and offers the opportunity to preserve the exceptional qualities of chitin and its derivatives. however, the major disadvantage of the fermentative way is the low efficiency of demineralization and deproteinization. the aim of this study is to improve the yield of extraction which depends on many factors, such as the medium composition and the physical parameters. in ord ... | 2013 | 23831901 |
| a novel cephalosporin deacetylating acetyl xylan esterase from bacillus subtilis with high activity toward cephalosporin c and 7-aminocephalosporanic acid. | a cephalosporin deacetylating acetyl xylan esterase was cloned from the genomic dna of bacillus subtilis cicc 20034 and functionally expressed in escherichia coli. its gene contained an open reading frame of 957 bp encoding 318 amino acids with a calculated mass of 35,607 da, and it displayed significant identity to acetyl xylan esterases from bacillus sp. 916, b. subtilis 168, and bacillus pumilus cect5072. the enzyme was a native homohexamer but a trimer under the condition of 1% sodium dodecy ... | 2014 | 23828600 |
| an icebs1-like element may be associated with the extreme radiation and desiccation resistance of bacillus pumilus safr-032 spores. | comparisons of the genomes of bacillus pumilus safr-032 and the closely related type strain, b. pumilus atcc7061(t), exposed an extended region of non-homologous genes. a detailed examination of this region revealed the presence of an icebs1-like integrative conjugative element in safr-032. a similar element was subsequently located elsewhere in the atcc7061(t) genome. a detailed comparison of these elements and the icebs1 of b. subtilis revealed extremely rapid flux in gene content, genome orga ... | 2013 | 23812891 |
| expression and biochemical characterization of cold-adapted lipases from antarctic bacillus pumilus strains. | two lipase genes (bpl1 and bpl3) from antarctic bacillus pumilus strains were expressed in bacillus subtilis. both recombinant lipases bpl1 and bpl2 were secreted to the culture medium and their activities reached 3.5 u/ml and 5.0 u/ml, respectively. their molecular masses apparent using sds-page were 23 kda for bpl1 and 19 kda for bpl3. both lipases were purified to homogeneity using ammonium sulfate precipitation and hitrap sp ff column and superose 12 column chromatographies. the final specif ... | 2013 | 23770563 |
| utilization of low-pressure plasma to inactivate bacterial spores on stainless steel screws. | a special focus area of planetary protection is the monitoring, control, and reduction of microbial contaminations that are detected on spacecraft components and hardware during and after assembly. in this study, wild-type spores of bacillus pumilus safr-032 (a persistent spacecraft assembly facility isolate) and the laboratory model organism b. subtilis 168 were used to study the effects of low-pressure plasma, with hydrogen alone and in combination with oxygen and evaporated hydrogen peroxide ... | 2013 | 23768085 |
| enhanced phytotransformation of navy blue rx dye by petunia grandiflora juss. with augmentation of rhizospheric bacillus pumilus strain pgj and subsequent toxicity analysis. | this study reveals the beneficial synergistic phytoremediation potential of petunia grandiflora juss. with its rhizospheric bacterial isolate bacillus pumilus strain pgj to decolorize reactive navy blue rx (nbrx) dye by their active enzymatic machinery. in vitro cultures of p. grandiflora and b. pumilus gave 80.01% and 76.80% while their consortium decolorized nbrx up to 96.86% within 36 h. significant induction in the enzyme activities of lignin peroxidase (207%), tyrosinase (133%), laccase (16 ... | 2013 | 23743429 |
| [bacillus pumilus strains with inactivated genes for extracellular serine proteinases]. | | 2013 | 23718049 |
| antibiofilm activity of bacillus pumilus sw9 against initial biofouling on microfiltration membranes. | membrane biofouling, resulting from biofilm formation on the membrane, has become the main obstacle hindering wider application of membrane technology. initial biofouling proves to be crucial which involves early stages of microbial adhesion and biofilm formation. biological control of microbial attachment seems to be a promising strategy due to its high efficiency and eco-friendliness. the present study investigated the effects of a bacterium bacillus pumilus sw9 on controlling the initial foul ... | 2014 | 23715854 |
| wool-waste valorization: production of protein hydrolysate with high antioxidative potential by fermentation with a new keratinolytic bacterium, bacillus pumilus a1. | wool, a recalcitrant waste mainly composed of keratin, constituted a serious problem for the environment and was not effectively valorized. this study reported the optimization of wool-waste biodegradation by a new keratinolytic bacterium bacillus pumilus a1. the in vitro digestibility and the antioxidant potential of wool protein hydrolysate (wph) were also investigated. | 2013 | 23663724 |
| isolation and biochemical characterization of bacillus pumilus lipases from the antarctic. | lipase-producing bacterial strains were isolated from antarctic soil samples using the tricaprylin agar plate method. seven strains with relatively strong lipase activities were selected. all of them turned out to be bacillus pumilus strains by the 16s rrna gene sequence analysis. their corresponding lipase genes were cloned, sequenced, and compared. finally, three different bacillus pumilus lipases (bpl1, bpl2, and bpl3) were chosen. their amino acid sequence identities were in the range of 92- ... | 2013 | 23648856 |
| generation of biologically contained, readily transformable, and genetically manageable mutants of the biotechnologically important bacillus pumilus. | bacillus pumilus mutants were generated by targeted deletion of a set of genes eventually facilitating genetic handling and assuring biological containment. the well-defined and stable mutants do not form functional endospores due to the deletion of yqfd, an essential sporulation gene; they are affected in dna repair, as δuvrba rendered them uv hypersensitive and, thus, biologically contained; they are deficient for the uracil phosphoribosyl-transferase (δupp), allowing for 5-fluorouracil-based ... | 2013 | 23644770 |
| production of an alkaline protease using bacillus pumilus d3 without inactivation by sds, its characterization and purification. | abstract in this study, protease-producing capacity of bacillus pumilus d3, isolated from hydrocarbon contaminated soil, was evaluated and optimized. optimum growing conditions for b. pumilus d3 in terms of protease production were determined as 1% optimum inoculum size, 35 °c temperature, 11 ph and 48 h incubation time, respectively. stability studies indicated that the mentioned protease was stable within the ph range of 7-10.5 and between 30 °c and 40 °c temperatures. surprisingly, the activi ... | 2014 | 23638694 |
| pigmentation and sporulation are alternative cell fates in bacillus pumilus sf214. | bacillus pumilus sf214 is a spore forming bacterium, isolated from a marine sample, able to produce a matrix and a orange-red, water soluble pigment. pigmentation is strictly regulated and high pigment production was observed during the late stationary growth phase in a minimal medium and at growth temperatures lower than the optimum. only a subpopulation of stationary phase cells produced the pigment, indicating that the stationary culture contains a heterogeneous cell population and that pigme ... | 2013 | 23634224 |
| covalent immobilization of xylanase produced from bacillus pumilus sv-85s on electrospun polymethyl methacrylate nanofiber membrane. | polymethyl methacrylate (pmma) nanofiber membrane (nfm) was synthesized by an electrospinning technique. these membranes were utilized as a support for immobilization of xylanase enzyme to study its ph stability, thermal stability, and reusability. the morphology of aligned nfm was studied by optical microscopy and scanning electron microscopy. the pmma nfm was functionalized with phenylenediamine and activated with glutaraldehyde to yield an aldehyde group on its surface for covalent immobiliza ... | 2013 | 23586605 |
| streptomyces lunalinharesii strain 235 shows the potential to inhibit bacteria involved in biocorrosion processes. | four actinomycete strains previously isolated from brazilian soils were tested for their antimicrobial activity against bacillus pumilus lf-4 and desulfovibrio alaskensis ncimb 13491, bacteria that are well known to be involved in biofilm formation and biocorrosion. strain 235, belonging to the species streptomyces lunalinharesii, inhibited the growth of both bacteria. the antimicrobial activity was seen over a wide range of ph, and after treatment with several chemicals and heat but not with pr ... | 2013 | 23484107 |
| thermostable keratinase from bacillus pumilus ks12: production, chitin crosslinking and degradation of sup35nm aggregates. | production of thermostable keratinase from bacillus pumilus ks12 was enhanced up to seven fold by statistical methods. the enzyme was partially purified by ultrafiltration followed by thermal precipitation with purity of 3.2-fold and recovery of 89%. keratinase was immobilized using covalent method by crosslinking 2 mg protein (688 u/mg) onto 1g chitin activated with 2.5% (v/v) glutaraldehyde for 60 min. its comparative biochemical studies with that of free keratinase revealed the shift in optim ... | 2013 | 23425582 |
| the supernatant of bacillus pumilus sqr-n43 has antifungal activity towards rhizoctonia solani. | for clarification of the antagonistic mechanism of bacillus pumilus sqr-n43 (n43) against rhizoctonia solani q1, production of antibiotics by n43 was determined, and the effect of the antibiotics on the pathogen mycelium was microscopically observed. further more, the control efficiencies of the antifungal compounds on damping-off disease were investigated. the results obtained are listed as follows: n43 produced antibiotic substances towards r. solani q1 at logarithmic growth phase. the antibio ... | 2013 | 23417338 |
| development of a high-efficient transformation system of bacillus pumilus strain dx01 to facilitate gene isolation via gfp-tagged insertional mutagenesis and visualize bacterial colonization of rice roots. | a tn5 transposition vector, pmod-tet-egfp, was constructed and used for the random insertional mutagenesis of bacillus pumilus. various parameters were investigated to increase the transformation efficiency b. pumilus dx01 via tn5 transposition complexes (transposome): bacterial growth phase, type of electroporation buffer, electric field strength, and recovery medium. transformation efficiency was up to 3 × 10(4) transformants/μg of dna under the optimized electroporation conditions, and a tota ... | 2013 | 23334949 |
| improved activity and thermostability of bacillus pumilus lipase by directed evolution. | to improve enzymatic activity of bacillus pumilus lipases, dna shuffling was applied to two lipase genes from local b. pumilus isolates. using a high-throughput activity assay, the mutant with highest activity was selected. this chimeric mutant (l3-3), carrying two crossover positions and three point mutations, has a specific activity 6.4 and 8.2 times higher than the two parent enzymes. the mutant also is more tolerant to various detergents and organic solvents, and has a 9 times longer half-li ... | 2013 | 23313890 |
| biobleaching application of cellulase poor and alkali stable xylanase from bacillus pumilus sv-85s. | the potential of extracellular alkali stable and thermo tolerant xylanase produced by bacilluspumilus sv-85s through solid state fermentation was investigated in pulp bleaching in association with conventional bleaching using chlorine and chlorine dioxide. the biobleaching of kraft pulp with xylanase was the most effective at an enzyme dose of 10 iu/g oven dried pulp, ph 9.0 and 120 min incubation at 55 °c. under the optimized conditions, xylanase pretreatment reduced kappa number by 1.6 points ... | 2013 | 28324585 |
| isolation and characterization of indigenous endophytic bacteria associated with leaves of switchgrass (panicum virgatum l.) cultivars. | to isolate and characterize indigenous bacterial endophytes from cultivars of switchgrass and study their antimicrobial and growth promoting potential. | 2013 | 23190162 |
| [the novel adams-like microbial metalloendopeptidase]. | heterologous gene expression of extracellular minor metalloendopeptidase of bacillus pumilus 3-19 in protease-deficient b. subtilis strain has been studied. the fraction of enzyme in total pool of b. pumilus 3-19 secreted proteases composes less than 8%. the enzyme was isolated from culture liquid of recombinant strain, its primary structure was determined, physicochemical properties were investigated. it was concluded that secreted metallo endopeptidase of b. pumilus 3-19 represents the first p ... | 2012 | 23189558 |
| draft genome sequence of bacillus pumilus ba06, a producer of alkaline serine protease with leather-dehairing function. | bacillus pumilus ba06 was isolated from the proteinaceous soil and produced an extracellular alkaline protease with leather-dehairing function. the genome of ba06 was sequenced. the comparative genome analysis indicated that strain ba06 is different in genome from the other b. pumilus strains, with limited insertions, deletions, and rearrangements. | 2012 | 23144411 |
| [influence of bacteria of bacillus genus on the causative agent of bacterial cancer of tomatoes]. | it has been shown that bacteria of the genus bacillus inhibited the development of infection caused by clavibacter michiganensis subsp. michiganensis, in tomatoes. pre-sowing seed treatment with suspensions of bacillus subtilis imv b-7023 and bacillus pumilus 3 enhanced resistance of plants to bacterial disease of cancer, probably due to the synthesis of biologically active substances with antimicrobial properties by these bacteria. of the two strains of bacillus, differing by antagonist propert ... | 2013 | 23120989 |
| exiguobacterium oxidotolerans, a halotolerant plant growth promoting rhizobacteria, improves yield and content of secondary metabolites in bacopa monnieri (l.) pennell under primary and secondary salt stress. | brahmi (bacopa monnieri), an integral component of indian ayurvedic medicine system, is facing a threat of extinction owing to the depletion of its natural populations. the present study investigates the prospective of exploitation of halotolerant plant growth promoting rhizobacteria (pgpr) in utilising the salt stressed soils for cultivation of b. monnieri. the effects of two salt tolerant pgpr, bacillus pumilus (str2) and exiguobacterium oxidotolerans (str36) on the growth and content of bacos ... | 2013 | 23085953 |
| characterization of thermostable serine alkaline protease from an alkaliphilic strain bacillus pumilus mcas8 and its applications. | this study describes the characterization and optimization of medium components for an extracellular detergent, surfactant, organic solvent and thermostable serine alkaline protease produced by alkaliphilic bacillus pumilus mcas8 strain isolated from pulicat lake sediments, tamil nadu, india. the strain yielded maximum protease (2,214 u/ml) under optimized conditions: carbon source, citric acid-1.5 % (w/w); inducer, soyabean meal-2 % (w/w); ph 11.0; shaking condition 37 °c for 48 h. the enzyme h ... | 2012 | 23076563 |
| high-antibacterial activity of urtica spp. seed extracts on food and plant pathogenic bacteria. | the aim of this study was to comparatively evaluate antibacterial activities of methanol (metoh) and aqueous (dw) leaf (l), root (r) and seed (s) extracts of urtica dioica l. (ud; stinging nettle) and urtica pilulifera l. (up; roman nettle) on both food- and plant-borne pathogens, with total phenolic contents and dpph radical scavenging activities (drsa). metoh extracts of leaves and roots of u. dioica had the highest drsa. extracts with high antibacterial activity were in the order up-lmetoh (1 ... | 2013 | 23067263 |
| highly active β-xylosidases of glycoside hydrolase family 43 operating on natural and artificial substrates. | the hemicellulose xylan constitutes a major portion of plant biomass, a renewable feedstock available for conversion to biofuels and other bioproducts. β-xylosidase operates in the deconstruction of the polysaccharide to fermentable sugars. glycoside hydrolase family 43 is recognized as a source of highly active β-xylosidases, some of which could have practical applications. the biochemical details of four gh43 β-xylosidases (those from alkaliphilus metalliredigens qymf, bacillus pumilus, bacill ... | 2013 | 23053115 |
| effect of plant growth-promoting rhizobacteria (pgprs) on plant growth, yield, and quality of tomato (lycopersicon esculentum mill.) under simulated seawater irrigation. | to determine the effects of three pgprs on plant growth, yield, and quality of tomato under simulated seawater irrigation, a two consecutive seasons' field experiment was conducted in yancheng teachers university plot from april to june and august to october, 2011. the results showed that erwinia persicinus ra2 containing acc deaminase exhibited the best ability compared with bacillus pumilus wp8 and pseudomonas putida rbp1 which had no acc deaminase activity to enhance marketable yields of fres ... | 2012 | 22990485 |
| antitrypanosomal alkaloids from the marine bacterium bacillus pumilus. | fractionation of the ethyl acetate extract of the marine bacterium bacillus pumilus isolated from the black coral antipathes sp. led to the isolation of five compounds: cyclo-(l-leu-l-pro) (1), 3-hydroxyacetylindole (2), n-acetyl-β-oxotryptamine (3), cyclo-(l-phe-l-pro) (4), and 3-formylindole (5). the structures of compounds 1-5 were established by spectroscopic analyses, including hresitof-ms and nmr (1h, 13c, hsqc, hmbc and cosy). compounds 2, 3 and 5 caused the inhibition on the growth of tr ... | 2012 | 22990456 |
| enhanced remediation of chlorpyrifos from soil using ryegrass (lollium multiflorum) and chlorpyrifos-degrading bacterium bacillus pumilus c2a1. | the combined use of plants and associated microorganisms has great potential for remediating soil contaminated with organic compounds such as pesticides. the objective of this study was to determine whether the bacterial inoculation influences plant growth promotion and chlorpyrifos (cp) degradation and accumulation in different parts of the plant. ryegrass was grown in soil spiked with cp and inoculated with a pesticide degrading bacterial strain bacillus pumilus c2a1. inoculation generally had ... | 2012 | 22959266 |
| biotechnological potential of agro residues for economical production of thermoalkali-stable pectinase by bacillus pumilus dcsr1 by solid-state fermentation and its efficacy in the treatment of ramie fibres. | the production of a thermostable and highly alkaline pectinase by bacillus pumilus dcsr1 was optimized in solid-state fermentation (ssf) and the impact of various treatments (chemical, enzymatic, and in combination) on the quality of ramie fibres was investigated. maximum enzyme titer (348.0 ± 11.8 ug(-1) dbb) in ssf was attained, when a mixture of agro-residues (sesame oilseed cake, wheat bran, and citrus pectin, 1 : 1 : 0.01) was moistened with mineral salt solution (a(w) 0.92, ph 9.0) at a su ... | 2012 | 22928091 |
| improving genetic immobilization of a cellulase on yeast cell surface for bioethanol production using cellulose. | in this study, saccharomyces cerevisiae was genetically engineered to harbor the capability of utilizing celluloses for bioethanol production by displaying active cellulolytic enzymes on the cell surface. an endo-1,4-β-glucanase gene egx was cloned from bacillus pumilus c-9 and its expression products, the egx cellulases, were displayed on the cell surface of s. cerevisiae by fusing egx with aga2 that encodes the binding subunit of the s. cerevisiae cell wall protein α-agglutinin. to achieve hig ... | 2013 | 22915066 |
| scale-up of an alkaline protease from bacillus pumilus mtcc 7514 utilizing fish meal as a sole source of nutrients. | fish meal grades sl1 and sl2 from sardine (sardinella longiceps) and nj from pink perch (nemipterus japonicas) were evaluated as a sole source of carbon and nitrogen in the medium for alkaline protease production by bacillus pumilus mtcc 7514. the analysis of the fish meal suggests that the carbon and nitrogen contents in fish meal are sufficient to justify its choice as replacement for other nutrients. protease production increased significantly (4,914 u/ml) in medium containing only fish meal, ... | 2012 | 22814497 |
| [subtilisin like protease secreted in bacillus pumilus kmm 62 on different growth stages]. | a protease secreted in bacillus pumilus kmm 62 culture liquid on different growth stages was isolated using ion-exchange chromatography. on the basis of pattern of specific chromogenic substrates hydrolysis and inhibitory analysis the protease was classified as subtilisin like serine protease. the molecular weight ofprotease is 31 kda. proteolytic activity towards z-ala-ala-leu-pna substrate was maximal at ph 8-8.5. the optimal temperature for proteolytic activity was observed at a temperature o ... | 2012 | 22792728 |
| heat and drying time modulate the o2 reduction current of modified glassy carbon electrodes with bilirubin oxidases. | here we show that the magnitude of the o(2) reduction current of cathodes based on bilirubin oxidases (bod) immobilized into a redox hydrogel strongly depends on the drying conditions such as the curing time and temperature of drying as well as the thermostability of the bod. to illustrate this effect, we performed experiments with two different bods: one labile bod from trachyderma tsunodae and one highly thermostable bod from bacillus pumilus with different preparation protocols. the balance b ... | 2012 | 22772078 |
| efficient pahs biodegradation by a bacterial consortium at flask and bioreactor scale. | in this work, the biodegradation of three polycyclic aromatic hydrocarbons (pahs) such as phenanthrene (phe), pyrene (pyr) and benzo[a]anthracene (baa) has been investigated. a bacterial consortium consisting of two strains was used for the first time based on preliminary promising biodegradation data. they were tentatively identified as staphylococcus warneri and bacillus pumilus. degradation values higher than 85% were obtained for each single pah when operating at flask scale, whereas minimum ... | 2012 | 22738812 |
| viability of phenanthrene biodegradation by an isolated bacterial consortium: optimization and scale-up. | in the present work, biodegradation of phenanthrene by a bacterial consortium (lb2), isolated from lab-polluted soils has been investigated. the 16s rrna gene-based molecular analysis revealed that the bacterial consortium lb2 consisted of two strains showing a very high homology with staphylococcus warneri and bacillus pumilus. the optimization of phenanthrene degradation by the consortium lb2, using a central composite face-centered design was carried out taking into account three important pa ... | 2013 | 22717666 |
| survival of bacillus pumilus spores for a prolonged period of time in real space conditions. | to prevent forward contamination and maintain the scientific integrity of future life-detection missions, it is important to characterize and attempt to eliminate terrestrial microorganisms associated with exploratory spacecraft and landing vehicles. among the organisms isolated from spacecraft-associated surfaces, spores of bacillus pumilus safr-032 exhibited unusually high resistance to decontamination techniques such as uv radiation and peroxide treatment. subsequently, b. pumilus safr-032 wa ... | 2012 | 22680694 |
| resistance of bacterial endospores to outer space for planetary protection purposes--experiment protect of the expose-e mission. | spore-forming bacteria are of particular concern in the context of planetary protection because their tough endospores may withstand certain sterilization procedures as well as the harsh environments of outer space or planetary surfaces. to test their hardiness on a hypothetical mission to mars, spores of bacillus subtilis 168 and bacillus pumilus safr-032 were exposed for 1.5 years to selected parameters of space in the experiment protect during the expose-e mission on board the international s ... | 2012 | 22680691 |
| nonrecovery of varying proportions of viable bacteria during spread plating governed by the extent of spreader usage and proposal for an alternate spotting-spreading approach to maximize the cfu. | to elucidate the cause of high variations and inconsistencies in bacterial cfu observed within and between different experiments while assessing viable bacterial counts through spread plating (sp). | 2012 | 22563785 |
| rapid detection of viable bacillus pumilus safr-032 encapsulated spores using novel propidium monoazide-linked fluorescence in situ hybridization. | the survival of bacillus pumilus safr-032 spores to standard industrial clean room sterilization practices necessitates the development of rapid molecular diagnostic tool(s) for detection and enumeration of viable bacterial spores in industrial clean room environments. this is of importance to maintaining the sterility of clean room processing products. this paper describes the effect of propidium monoazide (pma) on fluorescence in situ hybridization (fish) for detecting and enumerating b. pumil ... | 2012 | 22537819 |
| iodine from bacterial iodide oxidization by roseovarius spp. inhibits the growth of other bacteria. | microbial activities in brine, seawater, or estuarine mud are involved in iodine cycle. to investigate the effects of the microbiologically induced iodine on other bacteria in the environment, a total of 13 bacteria that potentially participated in the iodide-oxidizing process were isolated from water or biofilm at a location containing 131 μg ml(-1) iodide. three distinct strains were further identified as roseovarius spp. based on 16 s rrna gene sequences after being distinguished by restricti ... | 2013 | 22526798 |
| metabolic flexibility of d-ribose producer strain of bacillus pumilus under environmental perturbations. | the metabolic reaction rate vector is a bridge that links gene and protein expression alterations to the phenotypic endpoint. we present a simple approach for the estimation of flux distribution at key branch points in the metabolic network by using substrate uptake, metabolite secretion rate, and biomass growth rate for transketolase (tkt) deficient bacillus pumilus atcc 21951. we find that the glucose-6-phosphate (g6p) and pseudo catabolic/anabolic branch points are flexible in the d: -ribose- ... | 2012 | 22438109 |
| bilirubin oxidase from bacillus pumilus: a promising enzyme for the elaboration of efficient cathodes in biofuel cells. | a cota multicopper oxidase (mco) from bacillus pumilus, previously identified as a laccase, has been studied and characterized as a new bacterial bilirubin oxidase (bod). the 59 kda protein containing four coppers, was successfully over-expressed in escherichia coli and purified to homogeneity in one step. this 509 amino-acid enzyme, having 67% and 26% sequence identity with cota from bacillus subtilis and bod from myrothecium verrucaria, respectively, shows higher turnover activity towards bili ... | 2012 | 22410485 |
| isolation, purification and characterisation of low molecular weight xylanase from bacillus pumilus ssp-34. | low molecular weight endo-xylanase from bacillus pumilus ssp-34 was purified to homogeneity using ion exchange and size exclusion chromatographies. xylanases were isolated by novel purification protocol which includes the use of anion exchange matrix such as deae sepharose cl 6b with less affinity towards enzyme protein. the purified b. pumilus ssp-34 have a molecular weight of 20 kda, with optimum ph and temperature at 6.0 and 50 °c, respectively. the enzyme was stable at 50 °c for 30 min. it s ... | 2012 | 22350873 |
| pongamia pinnata seed cake: a promising and inexpensive substrate for production of protease and lipase from bacillus pumilus sg2 on solid-state fermentation. | the production of a protease and a lipase from bacillus pumilus sg2 on solid-state fermentation using pongamia pinnata seed cake as substrate was studied. the seed cake was proved to be a promising substrate for the bacterial growth and the enzyme production. the initial ph, incubation time and moisture content were optimized to achieve maximal enzyme production. maximum protease production was observed at 72 h and that of the lipase at 96 h of incubation. the production of protease (9840 u/g dm ... | 2011 | 22329247 |
| biodegradation of sewage wastewater using autochthonous bacteria. | the performance of isolated designed consortia comprising bacillus pumilus, brevibacterium sp, and pseudomonas aeruginosa for the treatment of sewage wastewater in terms of reduction in cod (chemical oxygen demand), bod (biochemical oxygen demand) mlss (mixed liquor suspended solids), and tss (total suspended solids) was studied. different parameters were optimized (inoculum size, agitation, and temperature) to achieve effective results in less period of time. the results obtained indicated that ... | 2012 | 22272181 |
| production of alkali tolerant cellulase free xylanase in high levels by bacillus pumilus sv-205. | the fermentation conditions were optimized for hyper production of xylanase from bacillus pumilus sv-205. the bacterium secretes high levels (7382.7±1200 iu/ml) of cellulase-free xylanase using wheat bran led to 21.63 fold increase in activity. a combination of yeast extract and peptone stimulated highest xylanase production (2448.0 iu/ml) as compared to other combinations. the most important characteristic of the enzyme is its high ph stability (100%) over a broad ph range of 6-11 for 24h. ther ... | 2012 | 22227307 |
| optimization of chitinase production by bacillus pumilus using plackett-burman design and response surface methodology. | a soil bacterium capable of degrading chitin on chitin agar plates was isolated and identified as bacillus pumilus isolate u5 on the basis of 16s rdna sequence analysis. in order to optimize culture conditions for chitinase production by this bacterium, a two step approach was employed. first, the effects of several medium components were studied using the plackett-burman design. among various components tested, chitin and yeast extract showed positive effect on enzyme production while mgso4 and ... | 2011 | 24250411 |
| evaluation of copper resistant bacteria from vineyard soils and mining waste for copper biosorption. | vineyard soils are frequently polluted with high concentrations of copper due application of copper sulfate in order to control fungal diseases. bioremediation is an efficient process for the treatment of contaminated sites. efficient copper sorption bacteria can be used for bioremoval of copper from contaminated sites. in this study, a total of 106 copper resistant bacteria were examined for resistance to copper toxicity and biosorption of copper. eighty isolates (45 from vineyard mollisol, 35 ... | 2011 | 24031606 |