| conjugative transfer, stability and expression of a plasmid encoding a cry1ac gene in bacillus cereus group strains. | the plasmid pht73 containing cry1ac and tagged with an erythromycin resistance gene was transferred from bacillus thuringiensis subspecies kurstaki kt0 to several bacillus cereus group strains by conjugation. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and phase contrast microscopy showed that the transconjugants containing plasmid pht73 could express cry1ac toxin and produce bipyramidal crystalline inclusion bodies during sporulation. the study demonstrated that pht73 c ... | 2004 | 14769465 |
| characterization of a diesel sludge microbial consortia for bioremediation. | the organisms found growing in a mixed diesel sludge-chromium metal waste were characterized directly using environmental scanning electron microscopy (esem) and traditional fixation techniques with scanning electron microscopy (sem). an attempt to identify organisms genetically directly from the sludge failed because of interference from chemicals in the waste with polymerase chain reaction (pcr). the organisms were isolated using plate culture techniques and isolates were characterized by sem ... | 2005 | 15712752 |
| the members of the bacillus cereus group are commonly present contaminants of fresh and heat-treated milk. | to determine the level of milk contamination by bacillus cereus sensu lato, 44 samples were collected from a dairy farm and two independent dairies in northeastern poland. a total of 680 b. cereus sensu lato isolates were recovered. based on spore counts, their highest level in milk was found during the spring and summer months. although significant variations in chromosomal dna polymorphisms among b. cereus sensu lato isolates were noted based on repetitive element sequence polymorphism (rep-pc ... | 2008 | 18456114 |
| the plasmid pbmbt1 from bacillus thuringiensis subsp. darmstadiensis (inta mo14-4) replicates by the rolling-circle mechanism and encodes a novel insecticidal crystal protein-like gene. | this work describes a novel rolling-circle replicating (rcr) plasmid pbmbt1 from bacillus thuringiensis subsp. darmstadiensis (inta mo14-4) encoding an insecticidal crystal protein-like gene. pbmbt1 (6700 bp) contains three orfs and their putative transcription initiation sites and shine-dalgarno sequences were localized. orf1 encodes a 34.6 kda protein which showed identity with the protein cryc53 from b. thuringiensis subsp. cameroun (24.6%), the cry15aa insecticidal crystal protein from b. th ... | 2005 | 15970328 |
| an activated by cobalt alkaline aminopeptidase from bacillus mycoides. | an intracellular arginine--specific aminopeptidase synthesized by bacillus mycoides was purified and characterized. the purification procedure for studied aminopeptidase consisted of ammonium sulphate precipitation and three chromatographic steps: anion exchange chromatography and gel permeation chromatography. a molecular weight of -50 kda was estimated for the aminopeptidase by gel permeation chromatography and sds-page. the optimal activity of the enzyme on arginyl-beta-naphthylamide as a sub ... | 2011 | 22808738 |
| insights into the genetic organization of the bacillus mycoides cryptic plasmids pdx14.2 and psin9.7 deduced from their complete nucleotide sequence. | bacillus mycoides, a member of the bacillus cereus group of bacteria, can be easily distinguished from close species because of colony shape, made by filaments of cells, resembling fungal hyphae, curving clock- or counterclockwise depending on the strain. two plasmids, one from a strain curving to the right (pdx14.2), the other from a strain curving to the left (psin9.7), were sequenced and analyzed for gene content and replication mode. rolling-circle replication modules and mobilization protei ... | 2005 | 16040120 |
| review of endogenous endophthalmitis during pregnancy including case series. | endogenous endophthalmitis during pregnancy is a rare condition with few reports of such infections in the peri- and post-partum period. we reviewed the literature on endogenous endophthalmitis in pregnancy and also report a series of four patients who presented to our institution from january 2011 to september 2011. we retrospectively reviewed four patients who developed endogenous endophthalmitis either during pregnancy (two patients) or in the post-partum period (two patients-one after aborti ... | 2012 | 23263732 |
| detection of toxigenic bacillus cereus and bacillus thuringiensis spores in u.s. rice. | bacillus cereus is a gram-positive, endospore forming pathogenic bacterium that is ubiquitous in the environment and is frequently associated with emetic and diarrheal types of foodborne illness. in this study, 178 samples of raw rice from retail food stores were analyzed for the presence of b. cereus spores. spores of bacillus species were found in 94 (52.8%) of the rice samples with an average concentration of 32.6 cfu/g (3.6-460 cfu/g for b. cereus and 3.6-23 cfu/g for bacillus thuringiensis) ... | 2009 | 19027973 |
| thermal resistance of spores from virulent strains of bacillus anthracis and potential surrogates. | the objective of this study was to determine the thermal resistance of spores of bacillus anthracis and potential surrogates. the heat resistance of spores suspended in buffer (ph 7.0 or 4.5), milk, or orange juice was determined at 70, 80, and 90 degrees c. d-values for b. anthracis strains sterne, vollum, and pasteur ranged from < 1 min at 90 degrees c to approximately 200 min at 70 degrees c and were lower under acidic than under neutral conditions. the d-values for b. anthracis spores fell w ... | 2005 | 16300074 |
| production of humic substances through coal-solubilizing bacteria. | in this paper, the production of humic substances (hs) through the bacterial solubilization of low rank coal (lrc) was evaluated. the evaluation was carried out by 19 bacterial strains isolated in microenvironments with high contents of coal wastes. the biotransformed lrc and the hs produced were quantified in vitro in a liquid growth medium. the humic acids (ha) obtained from the most active bacterial strain were characterized via elemental composition (c, h, n, o), ir analyses, and the e4/e6 r ... | 2014 | 25477925 |
| detection of antibiotic-related genes from bacterial biocontrol agents with polymerase chain reaction. | pseudomonas chlororaphis pa23, pseudomonas spp. strain df41, and bacillus amyloliquefaciens bs6 consistently inhibit infection of canola petals by sclerotinia sclerotiorum in both greenhouse and field experiments. bacillus thuringiensis bs8, bacillus cereus l, and bacillus mycoides s have shown significant inhibition against s. sclerotiorum on plate assays. the presence of antibiotic biosynthetic or self-resistance genes in these strains was investigated with polymerase chain reaction and, in on ... | 2006 | 16699573 |
| assessment of hemolytic activity, enzyme production and bacteriocin characterization of bacillus subtilis lr1 isolated from the gastrointestinal tract of fish. | in the present investigation, probiotic potential (antagonistic activity, enzyme production, hemolytic activity, biosafety, antibiotic sensitivity and bile tolerance level) of bacillus subtilis lr1 was evaluated. bacteriocin produced by the bacterial strain b. subtilis lr1 isolated from the gastrointestinal tract of labeo rohita was purified and characterized. the molecular weight of the purified bacteriocin was ~50 kda in 12 % native page and showed inhibitory activity against four fish pathoge ... | 2017 | 27590016 |
| [microbial composition of various surfaces of skin during develop- ment of atopic dermatitis based on data from maldi-tof mass-spectrometry identification method]. | study transformation of skin microflora during development of atopic dermatitis. | 2016 | 27228668 |
| sequence diversity of the bacillus thuringiensis and b. cereus sensu lato flagellin (h antigen) protein: comparison with h serotype diversity. | we set out to analyze the sequence diversity of the bacillus thuringiensis flagellin (h antigen [hag]) protein and compare it with h serotype diversity. some other bacillus cereus sensu lato species and strains were added for comparison. the internal sequences of the flagellin (hag) alleles from 80 bacillus thuringiensis strains and 16 strains from the b. cereus sensu lato group were amplified and cloned, and their nucleotide sequences were determined and translated into amino acids. the flagell ... | 2006 | 16820457 |
| material properties and antimicrobial activity of polyhydroxybutyrate (phb) films incorporated with vanillin. | polyhydroxybutyrate (phb) was produced by bacillus mycoides dfc 1, isolated from garden soil. antimicrobial (am) films of phb were prepared by incorporating vanillin (4-hydroxy-3-methoxybenzaldehyde) from 10 to 200 μg/g of phb. the films were assessed for antimicrobial activity against foodborne pathogens and spoilage bacteria comprising of escherichia coli, salmonella typhimurium, shigella flexneri, and staphylococcus aureus and fungi such as aspergillus flavus, aspergillus fumigatus, aspergill ... | 2015 | 26002697 |
| the small acid soluble proteins (sasp alpha and sasp beta) of bacillus weihenstephanensis and bacillus mycoides group 2 are the most distinct among the bacillus cereus group. | the bacillus cereus group includes bacillus anthracis, b. cereus, bacillus thuringiensis, bacillus mycoides and bacillus weihenstephanensis. the small acid soluble spore protein (sasp) beta has been previously demonstrated to be among the biomarkers differentiating b. anthracis and b. cereus; sasp beta of b. cereus most commonly exhibits one or two amino acid substitutions when compared to b. anthracis. sasp alpha is conserved in sequence among these two species. neither sasp alpha nor beta for ... | 2009 | 19616612 |
| inhibition of bacillus anthracis and potential surrogate bacilli growth from spore inocula by nisin and other antimicrobial peptides. | the ability of nisin, synthetic temporin analogs, magainins, defensins, and cecropins to inhibit bacillus anthracis, bacillus cereus, bacillus thuringiensis, bacillus mycoides, and bacillus subtilis growth from spore inocula was determined using well diffusion assays. nisin, magainin ii amide, and defensins were inhibitory in screening against b. anthracis sterne or b. cereus atcc 7004, but only nisin inhibited virulent b. anthracis strains. the mics of nisin against the 10 bacillus strains exam ... | 2006 | 17066940 |
| susceptibility of bacillus anthracis, bacillus cereus, bacillus mycoides, bacillus pseudomycoides and bacillus thuringiensis to 24 antimicrobials using sensititre automated microbroth dilution and etest agar gradient diffusion methods. | to examine susceptibilities of bacillus anthracis and related species to 24 antimicrobials using and concurrently comparing two methods. | 2007 | 17586563 |
| real-time pcr identification of unique bacillus anthracis sequences. | bacillus anthracis is a spore-forming, gram-positive microorganism. it is a causative agent of anthrax, a highly infectious disease. it belongs to the "bacillus cereus group", which contains other closely related species, including bacillus cereus, bacillus thuringiensis, bacillus mycoides, bacillus weihenstephanensis, and bacillus pseudomycoides. b. anthracis naturally occurs in soil environments. the ba5345 genetic marker was used for highly specific detection of b. anthracis with taqman probe ... | 2015 | 26667574 |
| detection of large plasmids from the bacillus cereus group. | the members of the bacillus cereus group, bacillus anthracis, bacillus thuringiensis, and b. cereus senso stricto, are largely defined by their content of large plasmids, which encode major virulence factors. here we offer an easy, fast, and reliable protocol for the isolation and detection of large plasmids up to the size of at least 350kb. furthermore, using this method, we report that bacillus mycoides contain large plasmids. | 2008 | 18179822 |
| in vitro antimicrobial effect of satureja wiedemanniana against bacillus species isolated from raw meat samples. | in this study a total of 30 raw meat samples obtained from ankara, turkey were screened for the presence of bacillus species. among the meat samples analyzed, the predominant species isolated was bacillus circulans; other bacillus species were identified as bacillus firmus, bacillus lentus, bacillus megaterium, bacillus licheniformis, bacillus mycoides, bacillus sphaericus, and bacillus cereus. minced meat samples were more contaminated with bacillus species than sliced beef sample. from these s ... | 2009 | 19735196 |
| multilocus sequence analysis of bacillus thuringiensis serovars navarrensis, bolivia and vazensis and bacillus weihenstephanensis reveals a common phylogeny. | the bacillus cereus group sensu lato includes six closely-related bacterial species: bacillus cereus, bacillus anthracis, bacillus thuringiensis, bacillus mycoides, bacillus pseudomycoides and bacillus weihenstephanensis. b. thuringiensis is distinguished from the other species mainly by the appearance of an inclusion body upon sporulation. b. weihenstephanensis is distinguished based on its psychrotolerance and the presence of specific signature sequences in the 16s rrna gene and cspa genes. a ... | 2013 | 23073664 |
| antimicrobial activity of extracts of the lichens cladonia furcata, parmelia caperata, parmelia pertusa, hypogymnia physodes and umbilicaria polyphylla. | the antimicrobial activity of acetone, methanol and aqueous extracts of the lichens cladonia furcata, parmelia caperata, parmelia pertusa, hypogymnia physodes and umbilicaria polyphylla is assessed. the extracts are tested on six species of bacteria and 10 species of fungi using the disk-diffusion method, and broth tube dilution is used to determine minimal inhibitory concentration (mic). the tested bacteria were more sensitive than the tested fungi. aqueous extracts of the investigated lichens ... | 2007 | 18236733 |
| glycosyltransferase: a specific marker for the discrimination of bacillus anthracis from the bacillus cereus group. | bacillus anthracis, the aetiological agent of anthrax, has been taxonomically classified with the bacillus cereus group, which comprises b. cereus, bacillus thuringiensis, bacillus mycoides, bacillus pseudomycoides and bacillus weihenstephanensis. although the pathogenesis and ecological manifestations may be different, b. anthracis shares a high degree of dna sequence similarity with its group member species. as a result, the discrimination of b. anthracis from its close relatives in the b. cer ... | 2008 | 18287289 |
| evaluation of antimicrobial activity of the lichens lasallia pustulata, parmelia sulcata, umbilicaria crustulosa, and umbilicaria cylindrica. | the antimicrobial properties of acetone, methanol, and aqueous extracts of the lichens lasallia pustulata, parmelia sulcata, umbilicaria crustulosa, and umbilicaria cylindrica were studied comparatively in vitro. antimicrobial activities of the extracts of different lichens were estimated by the disk diffusion test for gram-positive bacteria, gram-negative bacteria, and fungal organisms, as well as by determining the mic (minimal inhibitory concentration). the obtained results showed that the ac ... | 2007 | 18297873 |
| c. elegans behavior of preference choice on bacterial food. | caenorhabditis elegans is a free living soil nematode and thus in its natural habitat, c. elegans encounters many different species of soil bacteria. although some soil bacteria may be excellent sources of nutrition for the worm, others may be pathogenic. thus, we undertook a study to understand how c. elegans can identify their preferred food using a simple behavioral assay. we found that there are various species of soil bacteria that c. elegans prefers in comparison to the standard laboratory ... | 2009 | 19756391 |
| family portrait of bacillus cereus and bacillus weihenstephanensis cereulide-producing strains. | two thousand bacillus cereus sensu lato isolates from food and environmental matrices were screened by pcr for the presence of cereulide-producing strains. this survey identified 73 potential emetic strains, most of which originated from non-random food and clinical samplings. none of the 460 bacillus thuringiensis, bacillus mycoides and bacillus pseudomycoides strains were pcr-positive for the cereulide genetic determinants. the chromosomal and extrachromosomal gene pool diversity of a subset o ... | 2009 | 23765791 |
| development and validation of a real-time quantitative pcr assay for rapid identification of bacillus anthracis in environmental samples. | a real-time polymerase chain reaction (pcr) assay was developed for rapid identification of bacillus anthracis in environmental samples. these samples often harbor bacillus cereus bacteria closely related to b. anthracis, which may hinder its specific identification by resulting in false positive signals. the assay consists of two duplex real-time pcr: the first pcr allows amplification of a sequence specific of the b. cereus group (b. anthracis, b. cereus, bacillus thuringiensis, bacillus weihe ... | 2010 | 20827474 |
| identification and characterization of the multidrug resistance gene cfr in a panton-valentine leukocidin-positive sequence type 8 methicillin-resistant staphylococcus aureus iva (usa300) isolate. | the staphylococcal cfr gene mediates resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin a, a phenotype that has been termed phlops(a). the cfr gene has mainly been associated with coagulase-negative staphylococcal isolates from animals, and only a few cfr-positive methicillin-resistant staphylococcus aureus (mrsa) isolates have been described so far. this study reports the first description of a cfr-positive mrsa isolate (m05/0060) belonging to the pandemic ... | 2010 | 20921317 |
| antibacterial activity of the essential oil of satureja wiedemanniana against bacillus species isolated from chicken meat. | the antimicrobial activity of the essential oil (eo) of satureja wiedemanniana against bacillus spp. isolated from chicken meat was investigated. thirty-seven bacillus strains were isolated from 15 chicken meat samples and examined for proteolytic and lipolytic activities by agar well diffusion assay. of 37 bacillus isolated from raw chicken samples, which based on a clear zone diameter of ≥ 6 mm in agar well diffusion assays for proteolytic activity, 19 bacillus strains were selected for this s ... | 2010 | 20932089 |
| discrimination between bacillus species by impedance analysis of individual dielectrophoretically positioned spores. | we combine the use of dielectrophoretic positioning with electrical impedance measurements to detect and discriminate between individual bacterial spores on the basis of their electrical response. using lithographically defined microelectrodes, we use dielectrophoresis to manipulate individual bacterial spores between the electrodes. the introduction of a single spore between the microelectrodes produces a significant change in electrical response that is species-dependent. when positioned betwe ... | 2008 | 18407615 |
| bacillus manliponensis sp. nov., a new member of the bacillus cereus group isolated from foreshore tidal flat sediment. | a gram-positive, endospore-forming, new bacillus species, strain bl4-6(t), was isolated from tidal flat sediment of the yellow sea. strain bl4-6(t) is a straight rod, with motility by peritrichate flagella. the cell wall contains meso-diaminopimelic acid, and the major respiratory quinone is menaquinone-7. the major fatty acids are iso-c(15:0) and summed feature 3 (containing c(16:1) ω7c/iso-c(15:0) 2oh, and/or iso-c(15:0) 2oh/c(16:1) ω7c). cells are catalase-positive and oxidase-negative. the g ... | 2011 | 22203569 |
| draft genome sequence of bacillus mycoides m2e15, a strain isolated from the endosphere of potato. | we present the draft genome sequence of bacillus mycoides m2e15, a bacterium isolated from potato endosphere. analysis of the 6.08-mbp draft genome sequence identified 6,386 protein-encoding sequences, including potential plant growth promoting genes. specifically, genes for proteins involved in phosphate utilization, iron acquisition, and bacteriocin production were identified. | 2016 | 26950325 |
| effect of microorganisms on the plutonium oxidation states. | particular microbes from substrates at the low-level radioactive waste repository in the ignalina npp territory were exposed to (239)pu (iv) at low ph under aerobic conditions. pu(iii) and pu(iv) were separated and quantitatively evaluated using the modified anion exchange method and alpha spectrometry. tested bacteria bacillus mycoides and serratia marcescens were more effective in pu reduction than rhodococcus fascians. fungi paecillomyces lilacinus and absidia spinosa var. spinosa as well as ... | 2011 | 22112595 |
| phenolic content, antioxidant activity, antibacterial activity and phytochemical composition of garcinia lancifolia. | garcinia lancifolia (clusiaceae) is an unexplored medicinal plant used as stomachic, diuretic and its fruit is used to cure dysentery and diarrhoea. the acidic fruits are used to prepare juice, pickle and curries. the phytochemical analysis of different extracts of g. lancifolia leaf, stem and fruit revealed the presence of tannins, saponins, flavonoids, terpenoids, alkaloids and cardiac glycosides. the high phenolic content was observed in the methanol extract of leaf followed by methanol extra ... | 2012 | 23439879 |
| variation in the morphology of bacillus mycoides due to applied force and substrate structure. | response to mechanical force is a well characterised phenomenon in eukaryotic organisms, helping to organise multicellular structures. mechanotactic responses have only rarely been observed in prokaryotic taxa. this work reports on a morphological change due to variations in applied force and surface structure by bacillus mycoides flügge. b. mycoides is a ubiquitous soil organism well known among microbiologists for its characteristic spreading colony morphology. an apparent mechanotactic respon ... | 2013 | 24324702 |
| genome sequence of bacillus mycoides b38v, a growth-promoting bacterium of sunflower. | bacillus mycoides b38v is a bacterium isolated from the sunflower rhizosphere that is able to promote plant growth and n uptake. the genome of the isolate has approximately 5.80 mb and presents sequence codifiers for plant growth-promoting characteristics, such as nitrate reduction and ammonification and iron-siderophore uptake. | 2015 | 25838494 |
| induced sensitivity of bacillus subtilis colony morphology to mechanical media compression. | bacteria from several taxa, including kurthia zopfii, myxococcus xanthus, and bacillus mycoides, have been reported to align growth of their colonies to small features on the surface of solid media, including anisotropies created by compression. while the function of this phenomenon is unclear, it may help organisms navigate on solid phases, such as soil. the origin of this behavior is also unknown: it may be biological (that is, dependent on components that sense the environment and regulate gr ... | 2014 | 25289183 |
| delayed formation of zero-valent selenium nanoparticles by bacillus mycoides seite01 as a consequence of selenite reduction under aerobic conditions. | selenite (seo32-) oxyanion shows severe toxicity to biota. different bacterial strains exist that are capable of reducing seo32- to non-toxic elemental selenium (se0), with the formation of se nanoparticles (senps). these senps might be exploited for technological applications due to their physico-chemical and biological characteristics. the present paper discusses the reduction of selenite to senps by a strain of bacillus sp., seite01, isolated from the rhizosphere of the se-hyperaccumulator le ... | 2014 | 24606965 |
| use of titanium dioxide nanoparticles biosynthesized by bacillus mycoides in quantum dot sensitized solar cells. | one of the major challenges of nanotechnology during the last decade has been the development of new procedures to synthesize nanoparticles. in this context, biosynthetic methods have taken hold since they are simple, safe and eco-friendly. | 2014 | 25027643 |
| biological activities of two macroalgae from adriatic coast of montenegro. | in the present investigation the acetone extracts of macroalgae ulva lactuca and enteromorpha intestinalis were tested for antioxidant, antimicrobial and cytotoxic potential. antioxidant activity was evaluated by measuring the scavenging capacity of tested samples on dpph and superoxide anion radicals, reducing the power of samples and determination of total phenolic and flavonoid compounds in extracts. as a result of the study, u. lactuca extract was found to have a better free radical scavengi ... | 2015 | 26150743 |
| identification and analysis of informative single nucleotide polymorphisms in 16s rrna gene sequences of the bacillus cereus group. | analysis of 16s rrna genes is important for phylogenetic classification of known and novel bacterial genera and species and for detection of uncultivable bacteria. pcr amplification of 16s rrna genes with universal primers produces a mixture of amplicons from all rrna operons in the genome, and the sequence data generally yield a consensus sequence. here we describe valuable data that are missing from consensus sequences, variable effects on sequence data generated from nonidentical 16s rrna amp ... | 2016 | 27582514 |
| maldi-tof ms portrait of emetic and non-emetic bacillus cereus group members. | the number of foodborne intoxications caused by emetic bacillus cereus isolates has increased significantly. as such, rapid and reliable methods to identify emetic strains appear to be clinically relevant. in this study, intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) was used to differentiate emetic and non-emetic bacilli. the phyloproteomic clustering of 34 b. cereus emetic and 88 non-emetic isolates classified as b. cereus, bacillus thur ... | 2016 | 27196540 |
| crystal structure of the bacillus-conserved mazg protein, a nucleotide pyrophosphohydrolase. | ba1544 from bacillus anthracis was previously annotated as a transcription factor for the gene cluster ba1554 - ba1558, but has not been experimentally characterized. b. anthracis is an obligate pathogen causing fatal inhalational anthrax, and ba1544 is absolutely conserved in bacillus species, including bacillus cereus, bacillus thuringiensis and bacillus mycoides, with 100% sequence identity. to address the function of ba1544, we performed structural and biochemical studies, which revealed tha ... | 2016 | 26920050 |
| differentiation of strains from the bacillus cereus group by rflp-pfge genomic fingerprinting. | bacillus mycoides, bacillus pseudomycoides, bacillus weihenstephanensis, bacillus anthracis, bacillus thuringiensis, and bacillus cereus belong to the b. cereus group. the last three species are characterized by different phenotype features and pathogenicity spectrum, but it has been shown that these species are genetically closely related. the macrorestriction analysis of the genomic dna with the noti enzyme was used to generate polymorphism of restriction profiles for 39 food-borne isolates (b ... | 2013 | 23893780 |
| bacillus weihenstephanensis characteristics are present in bacillus cereus and bacillus mycoides strains. | the bacillus cereus group comprises seven bacterial species: bacillus cereus, bacillus anthracis, bacillus thuringiensis, bacillus mycoides, bacillus pseudomycoides, bacillus cytotoxicus, and bacillus weihenstephanensis. bacillus weihenstephanensis is distinguished based on its capability to grow at 7 °c but not at 43 °c, and the presence of specific signature sequences in the 16s rrna and cspa genes and in several housekeeping genes: glpf, gmk, purh, and tpi. bacillus weihenstephanensis-specifi ... | 2013 | 23413955 |
| microbial dynamics during shelf-life of industrial ricotta cheese and identification of a bacillus strain as a cause of a pink discolouration. | dairy products are perishable and have to be preserved from spoilage during the food chain to achieve the desired shelf-life. ricotta is a typical italian soft dairy food produced by heat coagulation of whey proteins and is considered to be a light and healthy product. the shelf-life of ricotta could be extended, as required by the international food trade market; however, heat resistant microflora causes spoilage and poses issues regarding the safety of the product. next-generation sequencing ( ... | 2016 | 27052696 |
| incidence, diversity and characteristics of spores of psychrotolerant spore formers in various repfeds produced in belgium. | the major objectives of this study were to determine the incidence of psychrotolerant spore formers from repfeds marketed in belgium, and their diversity and characteristics. spore formers in general were found as spores on 38.3% of the food samples and in 85% food products types evaluated. 76% of the food samples containing spore formers had spores before enrichment. a total of 86 spore formers were isolated from the samples. 28 of 86 bacterial spore formers (32.6%) were capable of vegetative g ... | 2014 | 25084675 |
| pcr detection of cytk gene in bacillus cereus group strains isolated from food samples. | a method for detection of the cytotoxin k cytk structural gene and its active promoter preceded by the plcr-binding box, controlling the expression level of this enterotoxin, was developed. the method was applied for the purpose of the analysis of 47 bacterial strains belonging to the bacillus cereus group isolated from different food products. it was found that the majority of the analyzed strains carried the fully functional cytk gene with its plcr regulated promoter. the cytk gene was not det ... | 2013 | 24060693 |
| localization of new peptidoglycan at poles in bacillus mycoides, a member of the bacillus cereus group. | bacillus mycoides is a sporogenic gram-positive soil bacillus of the b. cereus group. this bacillus, which forms hyphal colonies, is composed of cells connected in filaments that make up bundles and turn clock- or counterclockwise depending on the strain. a thick peptidoglycan wall gives the rod cells of these bacilli strength and shape. one approach used to study peptidoglycan neoformation in gram positives exploits the binding properties of antibiotics such as vancomycin and ramoplanin to nasc ... | 2012 | 22773111 |
| crystal structure and properties of the copper(ii) complex of sodium monensin a. | the preparation and structural characterization of a new copper(ii) complex of the polyether ionophorous antibiotic sodium monensin a (monna) are described. sodium monensin a binds cu(ii) to produce a heterometallic complex of composition [cu(monna)(2)cl(2)].h(2)o, 1. the crystallographic data of 1 show that the complex crystallizes in monoclinic space group c2 with cu(ii) ion adopting a distorted square-planar geometry. copper(ii) coordinates two anionic sodium monensin ligands and two chloride ... | 2009 | 19748125 |
| antimicrobial activity of biogenically produced spherical se-nanomaterials embedded in organic material against pseudomonas aeruginosa and staphylococcus aureus strains on hydroxyapatite-coated surfaces. | in an effort to prevent the formation of pathogenic biofilms on hydroxyapatite (ha)-based clinical devices and surfaces, we present a study evaluating the antimicrobial efficacy of spherical biogenic se-nanostructures embedded in organic material (bio se-nemo-s) produced by bacillus mycoides selte01 in comparison with two different chemical selenium nanoparticle (senp) classes. these nanomaterials have been studied as potential antimicrobials for eradication of established ha-grown biofilms, for ... | 2017 | 28233476 |
| identification of antifungal antibiotics of bacillus species isolated from different microhabitats using polymerase chain reaction and maldi-tof mass spectrometry. | although many bacillus species are known to be good antibiotic producers capable of acting as biocontrol agents, the underlying antimicrobial mechanisms are often poorly understood. in this study, 21 bacillus strains, demonstrating over 50% mycelial inhibition against sclerotinia sclerotiorum as well as significant control in plant assays, were examined for the presence of antibiotic biosynthetic genes. primers specific for bacillomycin d, iturin a, surfactin, mycosubtilin, fengycin, and zwitter ... | 2009 | 19898544 |
| development of an efficient electroporation method for rhizobacterial bacillus mycoides strains. | in order to develop a method for electroporation of environmental bacillus mycoides strains, we optimized several conditions that affect the electroporation efficiency of this bacterium. by combining the optimized conditions, the electroporation efficiency of strain ec18 was improved to (1.3±0.6)×10(5)cfu/μg dna, which is about 10(3)-fold increase in comparison with a previously reported value. the method was further validated on various b. mycoides strains, yielding reasonable transformation ef ... | 2017 | 28042055 |
| iodide accumulation by aerobic bacteria isolated from subsurface sediments of a 129i-contaminated aquifer at the savannah river site, south carolina. | (129)i is of major concern because of its mobility in the environment, excessive inventory, toxicity (it accumulates in the thyroid), and long half-life (~16 million years). the aim of this study was to determine if bacteria from a (129)i-contaminated oxic aquifer at the f area of the u.s. department of energy's savannah river site, sc, could accumulate iodide at environmentally relevant concentrations (0.1 µm i(-)). iodide accumulation capability was found in 3 out of 136 aerobic bacterial stra ... | 2011 | 21278282 |
| mutually exclusive distribution of the sap and eag s-layer genes and the lytb/lyta cell wall hydrolase genes in bacillus thuringiensis. | recently, two bacillus thuringiensis strains were reported to synthesize parasporal inclusion bodies made not of the expected crystal (cry) proteins but rather of the surface layer proteins (slp) sap (encoded by sap) and ea1 (encoded by eag), respectively. whether the presence of the sap and eag genes is restricted to these two b. thuringiensis strains or ubiquitous in b. thuringiensis is unknown. we report here the distribution of the sap and eag genes in b. thuringiensis. strains in the bacill ... | 2011 | 21611767 |
| application of ultrasound in combination with heat and pressure for the inactivation of spore forming bacteria isolated from edible crab (cancer pagurus). | this research was performed to characterize the resistance of three different bacterial spore species isolated from pasteurized edible crab (cancer pagurus) meat to heat treatments and to assess the potential of manosonication (ms) and manothermosonication (mts) as an alternative for their inactivation. the spore-forming bacteria used in this study were bacillus mycoides, bacillus weihenstephanensis and psychrobacillus psychrodurans. the thermal resistance of these three species was determined a ... | 2016 | 26874861 |
| total synthesis and stereochemical assignment of the antimicrobial lipopeptide cerexin a1. | the isolation and total synthesis of the antimicrobial lipopeptide cerexin a1 is reported. this synthesis includes the preparation of orthogonally protected γ-hydroxylysine, utilizing a nitrile reformatsky-type reaction as a key step to yield both diastereomers more efficiently than previously reported methods. the configuration of the β-hydroxyl in the lipid tail was determined by the use of a modified ohrui-akasaka approach. furthermore, new cerexin analogues from bacillus mycoides atcc 21929 ... | 2015 | 26465807 |
| [diversity of bacillus species inhabiting on the surface and endophyte of lichens collected from wuyi mountain]. | the present work reported the isolation, identification and diversity of bacillus species colonizing on the surface and endophyte in lichens collected from wuyi mountain. | 2015 | 26259479 |
| functional gene-based discovery of phenazines from the actinobacteria associated with marine sponges in the south china sea. | phenazines represent a large group of nitrogen-containing heterocyclic compounds produced by the diverse group of bacteria including actinobacteria. in this study, a total of 197 actinobacterial strains were isolated from seven different marine sponge species in the south china sea using five different culture media. eighty-seven morphologically different actinobacterial strains were selected and grouped into 13 genera, including actinoalloteichus, kocuria, micrococcus, micromonospora, mycobacte ... | 2015 | 25820602 |
| production of bioactive tryptamine derivatives by co-culture of marine streptomyces with bacillus mycoides. | tryptamine derivatives such as tryptamine and bacillamides were strong algicidal compounds promising in controlling harmful algae blooms, but their bioactivity and application researches were hindered by extremely low natural production rates. this study found an induced production of algicidal tryptamine derivatives by co-culture of marine streptomyces with bacillus mycoides, and optimised the culture method through changing important factors such as medium nutrition content, culture mode and p ... | 2015 | 25643750 |
| isolation and screening of probiotic candidates from marron, cherax cainii (austin, 2002) gastrointestinal tract (git) and commercial probiotic products for the use in marron culture. | six strains of bacteria including bacillus mycoides (a10) and shewanella species (a12) isolated from healthy marron intestine, bacillus species (pm1), bacillus subtilis (pm3), bacillus sp. (pm4) and bacillus sp. (aq) from commercial probiotic products were investigated for probiotic potential in marron culture. antibiotic susceptibility tests indicated pm3 and pm4 were susceptible to all nine antibiotics evaluated. a10, a12 and aq were resistant to class penicillins, whereas pm1 was resistant to ... | 2015 | 24917311 |
| immunological responses of customised probiotics-fed marron, cherax tenuimanus, (smith 1912) when challenged with vibrio mimicus. | a two-phased experiment was conducted to investigate the effects of dietary supplementation of customised probiotics on marron physiology. during the first phase marron were fed probiotic supplemented feed for 70 days, while in phase two the same marron were challenged with vibrio mimicus and their physiological responses were investigated for 4 days post-challenged. the experiment was carried out in a purpose-built room, designed for aquaculture research, using 18 of 250 l cylindrical plastic t ... | 2013 | 23643874 |
| transcriptional analysis of ftsz within the dcw cluster in bacillus mycoides. | in bacillus mycoides, as well as in other members of the b. cereus group, the tubulin-like protein of the division septum ftsz is encoded by the distal gene of the cluster division and cell wall (dcw). along the cluster the genes coding for structural proteins of the division apparatus are intermingled with those coding for enzymes of peptidoglycan biosynthesis, raising the possibility that genes with this different function might be coexpressed. transcription of ftsz in two model bacteria had b ... | 2013 | 23384289 |
| water treatment using activated carbon supporting silver and magnetite. | recent efforts in water purification have led to the development of novel materials whose unique properties can offer effective biocidal capabilities with greater ease of use and at lower cost. in this study, we introduce a novel procedure for the preparation of activated carbon (charcoal) composite in which magnetite and silver are incorporated (mcag); we also describe the use of this material for the disinfection of surface water. the formation process of magnetic mcag composite was studied us ... | 2012 | 23109597 |
| purification and biochemical characterization of a novel thermo-stable carboxymethyl cellulase from azorean isolate bacillus mycoides s122c. | bacillus mycoides s122c was identified as carboxymethyl cellulase (cmcellulase)-producing bacteria from the azorean bacillus collection (lab collection), which was isolated from local soil samples. the bacteria was identified by 16s rrna sequence and designated as b. mycoides s122c. ncbi blast analysis showed that the b. mycoides s122c 16s rrna sequence has high identity compared to other b. mycoides strains. cmcellulase was purified from the culture filtrates using anion-exchange chromatography ... | 2012 | 23070713 |
| escherichia coli noncoding rnas can affect gene expression and physiology of caenorhabditis elegans. | food and other environmental factors affect gene expression and behaviour of animals. differences in bacterial food affect the behaviour and longevity of caenorhabditis elegans. however, no research has been carried out to investigate whether bacteria could utilize endogenous rnas to affect c. elegans physiology. here we show that two escherichia coli endogenous noncoding rnas, oxys and dsra, impact on the physiology of c. elegans. oxys downregulates che-2, leading to impairment in c. elegans ch ... | 2012 | 23011127 |
| frequency and antimicrobial resistance of bacteria isolated from oral and topical medicaments from hilla, iraq. | the presence of microorganisms in pharmaceuticals is undesirable because they may cause spoilage of the product and may present an infection hazard to the consumers or patients. | 2012 | 22706191 |
| contrasts between subsurface microbial communities and their metabolic adaptation to polycyclic aromatic hydrocarbons at a forested and an urban coal-tar disposal site. | the abundance and distribution of microorganisms and their potential for mineralizing polycyclic aromatic hydrocarbons (pahs) were measured in subsurface sediment samples at two geographically separate buried coal-tar sites. at a relatively undisturbed forested site in the northeastern united states, metabolic adaptation to the pahs was evident: radiolabeled naphthalene and phenanthrene were converted to (14)co2 in core material from inside but not outside a plume of groundwater contamination. h ... | 1992 | 24193137 |
| bacterial pathogens and health problems of acipenser gueldenstaedtii and acipenser baerii sturgeons reared in the eastern black sea region of turkey. | farming sturgeons is an economically important practice in a number of asian and european countries. however, since it is not widely implementedin turkey, fertilized eggs necessary for research and industrial activities are imported from germany. due to the interest of several fish farms in culturing sturgeon in turkey and the lack of relevant data, this study investigated bacteria related health problems of two different sturgeon species, the diamond sturgeon (acipenser gueldenstaedtii) and the ... | 2017 | 28580011 |
| recovery of bacillus mycoides, b. pseudomycoides and aeromonas hydrophila from common carp (cyprinus carpio) and rainbow trout (oncorhynchus mykiss) with gill disease. | during a 3-month period from june to the end of august 2016, ~5% mortalities were observed in a farm with rainbow trout (oncorhynchus mykiss walbaum) and one farm of common carp (cyprinus carpio l.) in bulgaria. the disease was manifested by gill ulcers/rot, asphyxiation and bloody ascites. aeromonas hydrophila was isolated from the internal organs of all the diseased fish. bacillus mycoides or b. pseudomycoides were recovered from the gill lesions on diseased carp and rainbow trout, respectivel ... | 2017 | 28745820 |
| comparative transcriptomics of bacillus mycoides strains in response to potato-root exudates reveals different genetic adaptation of endophytic and soil isolates. | plant root secreted compounds alter the gene expression of associated microorganisms by acting as signal molecules that either stimulate or repel the interaction with beneficial or harmful species, respectively. however, it is still unclear whether two distinct groups of beneficial bacteria, non-plant-associated (soil) strains and plant-associated (endophytic) strains, respond uniformly or variably to the exposure with root exudates. therefore, bacillus mycoides, a potential biocontrol agent and ... | 2017 | 28824604 |
| inactivation of pathogenic microorganisms in freshwater using hso5(-)/uv-a led and hso5(-)/m(n+)/uv-a led oxidation processes. | freshwater disinfection using photolytic and catalytic activation of peroxymonosulphate (pms) through pms/uv-a led and pms/m(n+)/uv-a led [m(n+) = fe(2+) or co(2+)] processes was evaluated through the inactivation of three different bacteria: escherichia coli (gram-negative), bacillus mycoides (sporulated gram-positive), staphylococcus aureus (non-sporulated gram-positive), and the fungus candida albicans. photolytic and catalytic activation of pms were effective in the total inactivation of the ... | 2017 | 28651081 |
| antimicrobial activity of extracts of the lichens cladonia furcata, parmelia caperata, parmelia pertusa, hypogymnia physodes and umbilicaria polyphylla. | the antimicrobial activity of acetone, methanol and aqueous extracts of the lichens cladonia furcata, parmelia caperata, parmelia pertusa, hypogymnia physodes and umbilicaria polyphylla is assessed. the extracts are tested on six species of bacteria and 10 species of fungi using the disk-diffusion method, and broth tube dilution is used to determine minimal inhibitory concentration (mic). the tested bacteria were more sensitive than the tested fungi. aqueous extracts of the investigated lichens ... | 2007 | 28696892 |
| biogenic selenium nanoparticles: characterization, antimicrobial activity and effects on human dendritic cells and fibroblasts. | tailored nanoparticles offer a novel approach to fight antibiotic-resistant microorganisms. we analysed biogenic selenium nanoparticles (senps) of bacterial origin to determine their antimicrobial activity against selected pathogens in their planktonic and biofilm states. senps synthesized by gram-negative stenotrophomonas maltophilia [sm-senps(-)] and gram-positive bacillus mycoides [bm-senps(+)] were active at low minimum inhibitory concentrations against a number of clinical isolates of pseud ... | 2016 | 27319803 |
| an amphiprotic novel chitosanase from bacillus mycoides and its application in the production of chitooligomers with their antioxidant and anti-inflammatory evaluation. | the objectives of this investigation were to produce a novel chitosanase for application in industries and waste treatment. the transformation of chitinous biowaste into valuable bioactive chitooligomers (cos) is one of the most exciting applications of chitosanase. an amphiprotic novel chitosanase from bacillus mycoides tku038 using squid pen powder (spp)-containing medium was retrieved from a taiwan soil sample, which was purified by column chromatography, and characterized by biochemical prot ... | 2016 | 27517920 |
| switching of the rotational direction of rhizoidal colonies in a newly isolated bacillus mycoides strain ko01. | bacillus mycoides are known to form rhizoidal colonies on solid medium. in this study, a new strain of b. mycoides, strain ko01, was isolated from soil. genetic and growth patterns indicated that this strain belongs to subgroup ii of the b. cereus group. strain ko01 forms extensive rhizoidal colonies with predictable directions of rotation. the concentration of the agar, and not the chemical composition, altered the direction of the colony rotation, switching from counterclockwise to clockwise. ... | 2016 | 27118071 |
| genetic structure and diversity within local populations of bacillus mycoides. | sixty strains of bacillus mycoides were isolated from each of two sites and characterized by their responses to standard metabolic tests used in bacterial taxonomy, by multilocus enzyme electrophoresis (mlee), and by restriction-fragment-length polymorphism (rflp) analysis of southern blots probed with both a conserved dna fragment derived from a salmonella typhimurium ribosomal cistron and with two cosmid probes derived from b. mycoides atcc strain 6463. both mlee and rflp analyses indicated th ... | 1994 | 28568417 |