Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| analysis of cyclomaltodextrin glucanotransferase isoenzymes by isoelectric focusing in immobilized ph gradients. | the catalytically active subforms of cyclomaltodextrin glucanotransferase (cgtase; ec 2.4.1.19) from bacillus circulans var. alkalophilus and from a strain in which the cgtase expressing gene had been cloned were studied by using isoelectric focusing (ief) in immobilized and in conventional ph gradients. even with high protein loads the best resolution was achieved in immobilized ph gradients (ipg). native cgtase, focused on ipg 4.5-5.4, was resolved into more than 6 subforms, a major one with p ... | 1990 | 2140579 |
| cloning of two genes from bacillus circulans wl-12 which encode 1,3-beta-glucanase activity. | two genes encoding distinct 1,3-beta-glucanases have been cloned from bacillus circulans and expressed in escherichia coli. a cosmid library of b. circulans wl-12 dna was constructed in the broad-host-range cosmid plafr1 and screened in e. coli for clones which exhibited 1,3-beta-glucanase activity. two 1,3-beta-glucanase-positive clones were identified which contained genes encoding two independent 1,3-beta-glucanases as shown by biochemical, physical and molecular analyses. the cosmids, design ... | 1990 | 2127800 |
| exopolysaccharide structure from bacillus circulans. | 1991 | 2001692 | |
| multiple domains in endoglucanase b (cenb) from cellulomonas fimi: functions and relatedness to domains in other polypeptides. | endoglucanase b (cenb) from the bacterium cellulomonas fimi is divided into five discrete domains by linker sequences rich in proline and hydroxyamino acids (a. meinke, c. braun, n. r. gilkes, d. g. kilburn, r. c. miller, jr., and r. a. j. warren, j. bacteriol. 173:308-314, 1991). the catalytic domain of 608 amino acids is at the n terminus. the sequence of the first 477 amino acids in the catalytic domain is related to the sequences of cellulases in family e, which includes procaryotic and euca ... | 1991 | 1938913 |
| complete amino acid sequence of endo-beta-n-acetylglucosaminidase from flavobacterium sp. | the complete amino acid sequence of endo-beta-n-acetylglucosaminidase from flavobacterium sp. has been determined by analysis of peptides after cleavage with lysyl endopeptidase, pepsin and chymotrypsin. the protein consists of a single polypeptide chain consisting of 267 amino acid residues and a molecular mass of 27972 da. the sequence of flavobacterium endo-beta-n-acetylglucosaminidase is very close to that of the streptomyces enzyme (endo-h), having 60% similarity and very similar hydropathy ... | 1991 | 1935974 |
| microbial metabolism of quinoline and related compounds. ix. degradation of 6-hydroxyquinoline and quinoline by pseudomonas diminuta 31/1 fa1 and bacillus circulans 31/2 a1. | two strains, using 6-hydroxyquinoline as sole source of energy, carbon and nitrogen, have been isolated. these bacteria, designated 31/1 fa1 and 31/2 a1, are also able to degrade quinoline. according to their physiological properties strain 31/1 fa1 has been identified as pseudomonas diminuta and strain 31/2 a1 as bacillus circulans. 6-hydroxy-2-oxo-1,2-dihydroquinoline was found as intermediate in the degradation of 6-hydroxyquinoline and quinoline. 2-oxo-1,2-dihydroquinoline was the first meta ... | 1991 | 1910576 |
| on the role of histidine residues in cyclodextrin glycosyltransferase: chemical modification with diethyl pyrocarbonate. | ethoxyformylation with diethyl pyrocarbonate of approximately 1.5 his residues per molecule of enzyme reduced the cyclising activity of both the alpha-cyclodextrin glycosyltransferase from klebsiella pneumoniae strain m 5 al and the beta-cyclodextrin glycosyltransferase from bacillus circulans strain 8 by greater than 90%. pre-incubation with substrate protected the enzymes from ethoxyformylation. digestion of starch by the modified enzymes resulted in a delayed formation of cyclodextrins (cyclo ... | 1991 | 1828005 |
| structure of cyclodextrin glycosyltransferase refined at 2.0 a resolution. | the previously reported structural model of cyclodextrin glycosyltransferase (ec 2.4.1.19) from bacillus circulans has been improved. for this purpose the known sequence was built into an electron density map established by multiple isomorphous replacement and subsequent solvent-flattening at 2.5 a resolution. the resulting model was refined at 2.0 a resolution using a simulated annealing refinement method. based on 70,171 independent reflections in the range 7.0 to 2.0 a resolution, a final r-f ... | 1991 | 1826034 |
| endocarditis caused by bacillus circulans. | 1991 | 1816117 | |
| structure of the gene encoding chitinase d of bacillus circulans wl-12 and possible homology of the enzyme to other prokaryotic chitinases and class iii plant chitinases. | the gene (chid) encoding the precursor of chitinase d was found to be located immediately upstream of the chia gene, encoding chitinase a1, which is a key enzyme in the chitinase system of bacillus circulans wl-12. sequencing analysis revealed that the deduced polypeptide encoded by the chid gene was 488 amino acids long and the distance between the coding regions of the chia and chid genes was 103 bp. remarkable similarity was observed between the n-terminal one-third of chitinase d and the c-t ... | 1992 | 1729234 |
| three n-terminal domains of beta-1,3-glucanase a1 are involved in binding to insoluble beta-1,3-glucan. | limited proteolysis of beta-1,3-glucanase a1 by three different proteases, trypsin, chymotrypsin, and papain, gave three major active fragments. the sizes of the three major fragments generated by each protease treatment were identical to those of beta-1,3-glucanase a2, a3, and a4 detected in both the culture supernatant of bacillus circulans wl-12 and the periplasmic space of escherichia coli carrying a cloned glca gene. these results indicate a four-domain structure for the enzyme. at the n te ... | 1992 | 1729208 |
| the chromosomal integration site of the streptomyces element psam2 overlaps a putative trna gene conserved among actinomycetes. | the psam2 element of streptomyces ambofaciens integrates site-specifically in the genome of different streptomyces species by recombination between a 58 bp sequence common to the plasmid (attp) and the chromosome (attb). southern hybridization analysis showed that sequences similar to the psam2 attb site were found in other actinomycetes (mycobacterium, nocardia, micromonospora) as well as unrelated bacteria (bacillus circulans, escherichia coli, clostridium botulinum, bordetella pertussis, and ... | 1990 | 1703270 |
| purification and characterization of alpha-l-fucosidase from bacillus circulans grown on porcine gastric mucin. | bacillus circulans isolated from soil was found to produce two types of alpha-l-fucosidase differing in substrate specificity. one was able to liberate l-fucose from porcine gastric mucin (pgm), but not from artificial substrates, including p-nitrophenyl and methyl alpha-l-fucosides, while the other acted not on pgm but on p-nitrophenyl alpha-l-fucoside. the production of the former enzyme was enhanced about 150 times as much by pgm added to the medium as by glucose. the alpha-l-fucosidase actin ... | 1990 | 1694531 |
| characterisation of bcibii, an isoschizomer of bstni from a strain of bacillus circulans b. | 1992 | 1614883 | |
| isolation and partial characterization of an 87-kilodalton beta-1,3-glucanase from bacillus circulans iam1165. | bacillus circulans iam1165 produces at least two extracellular beta-1,3-glucanases that lyse fungal cell walls. one of these extracellular enzymes was purified to homogeneity. the molecular mass was 87 kda, and the pi was 4.3. the optimum temperature of the enzyme reaction was 70 degrees c when laminarin (a soluble beta-1,3-glucan) was used as the substrate. the ph range of the enzyme was broad (ph 4.5 to 9.0), and the optimum ph was 6.5. the enzyme is an endo beta-1,3-glucanase and has a random ... | 1992 | 1610176 |
| cyclomaltodextrin glucanotransferase from bacillus circulans e 192. i. purification and characterization of the enzyme. | the cyclomaltrodextrin glucanotransferase (cgtase) [1,4-alpha-d-glucan:4-alpha-d-(1,4-alpha-d-glucano)-transferase (cyclizing), ec 2.4.1.19] from bacillus circulans e 192 has been purified to homogeneity by cetavlon treatment, ammonium sulfate precipitation, deae trisacryl m chromatography, q fast flow chromatography, and affinity on beta-cyclodextrin-sepharose 4b. two isoenzymes were separated by fplc on a mono q column. their isoelectric points were estimated as 6.7 and 6.9 and they represente ... | 1992 | 1532314 |
| purification and some properties of l-fucose dehydrogenase from agrobacterium radiobacter and its application to the assay of bound-fucose in glycoconjugates. | l-fucose dehydrogenase was found in the cell extract of agrobacterium radiobacter and purified to homogeneity about 480-fold with 16% recovery. the molecular weight of the enzyme was approx. 64,000. the enzyme was active in the neutral ph range, unlike other l-fucose or d-arabinose dehydrogenases which are active only in the alkaline ph range. using this enzyme and alpha-l-fucosidase f-i of bacillus circulans (tsuji, y., yamamoto, k., tochikura, t., seno, t., ohkubo, y. and yamaguchi, h. (1990) ... | 1992 | 1525177 |
| purification, properties, and partial amino acid sequence of chitinase from a marine alteromonas sp. strain o-7. | chitinase (ec 3.2.1.14) was isolated from the culture supernatant of a marine bacterium, alteromonas sp. strain o-7. the enzyme (chi-a) was purified by anion-exchange chromatography (deae-toyopearl 650 m) and gel filtration (sephadex g-100). the purified enzyme showed a single band on sodium dodecyl sulfate polyacrylamide gel electrophoresis. the molecular size and pi of chi-a were 70 kda and 3.9, respectively. the optimum ph and temperature of chi-a were 8.0 and 50 degrees c, respectively. chi- ... | 1992 | 1464065 |
| primary structure of a chitinase-encoding gene (chi1) from the filamentous fungus aphanocladium album: similarity to bacterial chitinases. | chitinase 1 (chi1) is the major extracellular chitinase from the hyperparasitic fungus, aphanocladium album. we determined the complete sequence of the chromosomal and cdna copies of the structural gene (chi1) coding for chi1. the coding region is interrupted by three short introns (55, 53 and 49 bp long). chi1 is 423 aa long and begins with a stretch of 34 aa not found in the mature protein. the chi1 sequence presents overall similarities with bacterial chitinases from serratia marcescens and b ... | 1992 | 1398137 |
| catalytic center of cyclodextrin glycosyltransferase derived from x-ray structure analysis combined with site-directed mutagenesis. | an x-ray structure analysis of a crystal of mutant asp229----ala of cyclodextrin glycosyltransferase from bacillus circulans (ec 2.4.1.19) that had been shortly exposed to beta-cyclodextrin showed density corresponding to a maltose bound at the catalytic center. the crystal structure was refined to an r-factor of 18.7% at 2.5-a resolution. the catalytic center is defined by homology with the structurally known alpha-amylases and by the observation that mutants asp229----ala and asp328----ala are ... | 1992 | 1390660 |
| chemical modification of cyclomaltodextrin glucanotransferase from bacillus circulans var. alkalophilus. | counting of integral numbers of cysteine residues of the reduced and denaturated form of cyclomaltodextrin glucanotransferase (cgtase) from bacillus circulans var. alkalophilus (atcc 21783) showed two cysteine residues per enzyme molecule. titrations of the enzyme with 5,5'-dithiobis-(2-nitrobenzoic acid) led to the same result. no free sh-group was detected in denatured form of cgtase, indicating that the two cysteine residues are linked by one disulfide bridge. cyclizing activity of the gdmcl- ... | 1992 | 1385982 |
| [selection and physiological study of culture of bacillus circulans-- producer of butirosin]. | for isolating a highly active variant of the butirosin-producing culture, a strain forming trace amounts of the antibiotic substance was used. exposure to nitrosomethylbiuret and nitrosoguanidine and the use of selective media containing streptomycin and butirosin resulted in a 30-fold increase in the strain productivity. thin layer chromatography of the produced antibiotic substance in the solvent system developed by the authors, mass spectrometry and assay of the antimicrobial spectrum in rega ... | 1992 | 1384451 |
| proteolytic modification of raw-starch-digesting amylase from bacillus circulans f-2 with subtilisin: separation of the substrate-hydrolytic domain and the raw substrate-adsorbable domain. | raw starch-digesting amylase (bf-2a, 93,000 da) from bacillus circulans f-2 was converted into two components during digestion with subtilisin. the two components were separated and designated bf-2a' (63 kda) and bf-2b (30 kda), respectively. bf-2a' exhibited the same hydrolysis curve for soluble starch as the original amylase (bf-2a). moreover, the catalytic activities of original and modified enzymes were indistinguishable in km, vmax and in their specific activity for soluble starch hydrolysi ... | 1992 | 1380302 |
| enzymatic synthesis of p-nitrophenyl 4(5)-o-beta-d-galactosyl-alpha-maltopentaoside as a substrate for human alpha-amylases. | enzymatic modification at the nonreducing end d-glucosyl residue of p-nitrophenyl alpha-maltopentaoside was developed by using the transglycosylation of beta-d-galactosidase from bacillus circulans. the enzyme regioselectively synthesized p-nitrophenyl 4(5)-o-beta-d-galactosyl-alpha-maltopentaoside (a yield of 12.0% based on the amount of p-nitrophenyl alpha-maltopentaoside added) on a preparative scale from lactose as a donor and p-nitrophenyl alpha-maltopentaoside as an acceptor. it revealed t ... | 1992 | 1377891 |
| production of cyclomaltodextrin glucanotransferase of bacillus circulans var. alkalophilus atcc21783 in b. subtilis. | the cyclomaltodextrin glucanotransferase (cgtase, e.c. 2.4.1.19) gene from an alkalophilic bacillus circulans var. alkalophilus atcc21783 was cloned into escherichia coli and b. subtilis. when cloned from e. coli to b. subtilis, the entire insert containing the cgtase gene was, depending on the plasmid construction, either unstable or the recombinant b. subtilis did not secrete the enzyme in significant amounts. to achieve efficient enzyme production in b. subtilis, the gene was placed under the ... | 1992 | 1368772 |
| construction of an escherichia coli export-affinity vector for expression and purification of foreign proteins by fusion to cyclomaltodextrin glucanotransferase. | a novel export-affinity fusion vector employing the gene encoding cyclomaltodextrin glucanotransferase (cgtase; cgt) from bacillus circulans var. alkalophilus (atcc 21783) is described. cgtase binds to various sugar polymers, which makes it simple to purify it to near homogeneity in a single step. the cgtase fusion protein vector was constructed by deleting the translational stop codons from the gene encoding cgtase (cgt) by in vitro mutagenesis. as models, genes encoding escherichia coli alkali ... | 1992 | 1368771 |
| enzymic synthesis of 4(5)-o-beta-galactosyl-maltopentaose by bacillus circulans beta-galactosidase. | 1991 | 1368744 | |
| highly homologous cyclodextrin glycosyltransferases from bacillus circulans strain 8 and a strain of bacillus licheniformis. | 1990 | 1368625 | |
| molecular cloning, nucleotide sequence and expression in escherichia coli of the beta-cyclodextrin glycosyltransferase gene from bacillus circulans strain no. 8. | the beta-cyclodextrin glycosyltransferase (beta-cgtase) gene was isolated from a lambda-library prepared from bacillus circulans strain no. 8. it was subcloned into plasmid ptz and expressed by its endogenous regulatory sequences in escherichia coli jm 103. the structural gene was sequenced and showed an open reading frame for a polypeptide of 718 amino acid residues. the recombinant beta-cgtase had the same enzymatic properties as the extracellular cgtase (684 amino acid residues, corresponding ... | 1990 | 1368573 |
| galactosylation at side chains of branched cyclodextrins by various beta-galactosidases. | the galactosyl transfer reaction to branched cyclodextrins (cds) was investigated using lactose as a donor substrate and branched cds as acceptors by various beta-galactosidases. bacillus circulans beta-galactosidase synthesized galactosyl transfer products to branched cds, of which the galactose residues were linked at side chains of branched cds, not directly at cd rings. aspergillus oryzae and penicillium multicolor beta-galactosidases also produced derivatives galactosylated at side chains o ... | 1992 | 1368300 |
| [cloning and expression of alpha-hydroxy-gamma-aminobutyl acylase gene of bacillus circulans nrrl-b3312]. | with shot-gun cloning strategy, we used pub110 plasmid as a vactor to clone dna fragment of bacillus circulans nrrl-b3312, which is butirosin producer, into bacllus subtilis 168. among the transformants, the results of tlc, bioautography and fab mass, spectrum analysis for the bioconversion product of no. 733 transformant showed that this transformant could transform kanamycin into amikacin. according to these results, the haba acylase gene locates on the insert fragment of pubc733 plasmid harbo ... | 1992 | 1305824 |
| peptidoglutaminase (bacillus circulans). | 1976 | 1012012 | |
| isolation of a new peptide antibiotic complex, b-43 (studies on antibiotics from the genus bacillus. xv). | a new peptide antibiotic complex b-43, active against gram-positive and gram-negative bacteria, was isolated from a strain of bacillus circulans. this antibiotic contains aspartic acid, valine, isoleucine, leucine, phenylalanine and 2,4-diaminobutyric acid. it seems to be related to polypeptin and antibiotic complex 4205, but differs in that it contains aspartic acid residue. | 1976 | 993119 |
| isolation of a new antibiotic 333-25, related to antibiotic em 49. (studies on antibiotics from the genus bacillus. xi). | a new antibiotic, 333-25, active against gram-positive and gram-negative bacteria, was isolated from the culture broth of bacillus circulans 333-25. the antibiotic is a basic acylpeptide containing 2,4-diaminobutyric acid (5), leucine (2), phenylalanine (1) and a fatty acid. it is closely related to antibiotic em 49, but can be differentiated by chromatographic behaviour. | 1976 | 956039 |
| microorganism capable of decomposing n-acetylglucosaminyl ribitol teichoic acid of staphylococcus aureus. | enzyme(s) capable of decomposing n-acetylglucosaminyl ribitol teichoic acid prepared from the cell wall of staphylococcus aureus fda 209 p was obtained from the culture supernatant of a gram-negative, rod-shaped, spore-forming soil bacterium. properties of the bacterium were very similar to those of bacillus circulans. | 1976 | 948143 |
| cell wall studies of histoplasma capsulatum and blastomyces dermatitidis using autologous and heterologous enzymes. | enzymes capable of hydrolyzing cell walls of blastomyces dermatitidis and chemotypes i and ii of histoplasma capsulatum were prepared in the laboratory or obtained from commercial sources. they included chitinases, beta-1,3-glucanases, beta-1,6-glucanase, and pronase. monosaccharides and disaccharides of glucose released from the cell walls by the enzymes were determined qualitatively by paper and gas-liquid chromatography, and monosaccharides were quantitated by the latter technique as well. an ... | 1977 | 870437 |
| chemical and physical properties of peptidoglutaminase i and ii from bacillus circulans. | some chemical and physical properties of peptidoglutaminase i and ii have been determined. for example, molecular weight, isoelectric point, intrinsic viscosity, partial specific volume, sedimentation and diffusion coefficient were 89 000 and 105 000, ph = 4.1 and 4.0, 0.042 and 0.065 dl/g, 0.733 and 0.728 ml/g, 5.85 and 5.3 s, 5.32 and 4.57-10(-7) cm2-s-1, respectively. their amino acid compositions were also determined. between them, peptidoglutaminanse ii was a glycoprotein containing 2 mol o ... | 1976 | 816382 |
| the structure of permetin a, a new polypeptin type antibiotic produced by bacillus circulans. | the structure of permetin a(i), an antibiotic substance produced by bacillus circulans aj 3902, has been elucidated as a cyclic acyl peptide by means of the mass and nuclear magnetic resonance spectroscopic techniques. (formula: see text.) the structure was found to be the same as polypeptin a(ii) except that l-thr in ii is replaced by l-ser in i. details of the structural determination are given for the permetin a itself as well as for the hydrolyzed permetin a. (formula: see text.) | 1979 | 438100 |
| isolation of a new peptide antibiotic, permetin a, from bacillus circulans. | permetin a was purified from the culture filtrate of bacillus circulans aj 3902 by extraction with n-butanol, precipitation with sodium helianthate, cm-cellulose column chromatography and sephadex lh-20 column chromatography. the compound was found to be a new peptide antibiotic containing 2,4-diaminobutyric acid (dab), leucine, isoleucine, phenylalanine, valine, serine (in a molar ratio of 3:2:1:1:1:1) and a fatty acid. this antibiotic showed activity in vitro against gram-negative, gram-positi ... | 1979 | 438099 |
| the enzymic degradation of an alkali-soluble glucan from the cell walls of saccharomyces cerevisiae. | an alkali-soluble glucan from the cell walls of saccharomyces cerevisiae ncyc1109 has been hydrolysed with a purified endo-(1 leads to 3)-beta-d-glucanase and an endo-(1 leads to 6)-beta-d-glucanase from bacillus circulans wl-12. the products of enzyme action include various oligosaccharide and polysaccharide fractions which have been separated by gel filtration and characterized, giving new information on the fine structure of the glucan. the isolated cell walls have also been subjected to enzy ... | 1977 | 323412 |
| aminoglycoside-modifying enzyme of an antibiotic-producing bacterium acts as a determinant of antibiotic resistance in escherichia coli. | bacillus circulans nrrl b-3312, a nonpathogenic bacterium that produces the aminoglycoside antibiotic butirosin, is known to contain an aminoglycoside phosphotransferase that is similar to the neomycin phosphotransferases of clinically isolated antibiotic-resistant bacteria. purified dnas from b. circulans and the plasmid cole1-apr were digested with ecori endonuclease and the resulting fragments covalently joined with polynucleotide ligase. the recombined dna was used to transform e. coli and a ... | 1977 | 322154 |
| structure of the peptide antibiotic polypeptin. | polypeptin, a basic peptide antibiotic isolated from bacillus circulans, was separated into two components by countercurrent distribution. the two components, polypeptin a and polypeptin b, had identical amino acid compositions but varied in the structure of the hydroxy acid constituent attached to the alpha-amino group of the peptide chain. polypeptin a contained 3-hydrosy-4-methylhexanoic acid and polypeptin b contained 3-hydrosy-5-methylhexanoic acid. t-he sterochemistry of these hydroxy acid ... | 1976 | 186604 |
| susceptibility to butirosin and neomycin b in bacillus circulans, the butirosin-producing organism. | butirosin, an aminoglycoside antibiotic, is produced by bacillus circulans b-3312. experiments using recombined ribosomal and supernatant fractions from this strain and from b. megaterium km have shown that the ribosome of both are sensitive to butirosin. the aminoglycoside 3'-phosphotransferase present in b. circulans modifies butirosin and neomycin in vitro but confers resistance only to the former in vivo. the phosphotransferase does not modifya detectable amount of extracellular butirosin wh ... | 1979 | 93616 |
| ribostamycin production by a mutant of butirosin producing bacteria. | by the use of our improved colony selection technique, xylostasin and ribostamycin producing mutants were isolated from nitrosoguanidine treated bacillus circulans b15m, a producer of butirosins a and b. among these structurally related aminoglycosides, ribostamycin is the well-known product of a steptomyces and has not been isolated as a bacterial metabolite. a selected mutant of strain 306, which produces xylostasin and ribostamycin, was futher mutagenized in expectation of getting an improved ... | 1978 | 81827 |
| mutational biosynthesis of butirosin analogs. iii. 6'-n-methylbutirosins and 3', 4'-dideoxy-6'-c-methylbutirosins, new semisynthetic aminoglycosides. | two pairs of butirosin analogs were isolated from the fermentation broths obtained by cultivating a neamine-negative mutant of the butirosin-producing organism bacillus circulans in the medium supplemented with 6'-n-methylneamine and gentamine c2, respectively. these amtibiotics were characterized as 6'-n-mentylbutirosins a and b (nmb-a & nmb-b), and 3', 4'-dideoxy-6'-c-methylbutirosins a and b (dcb-a & dcb-b), respectively, by chemical and spectroscopic studies. nmb-a and nmb-b exhibited broad- ... | 1978 | 81824 |
| mutational biosynthesis of butirosin analogs. ii. 3', 4'-dideoxy-6'-n-methylbutirosins, new semisynthetic aminoglycosides. | a pair of new butirosin analogs was isolated from the fermentation broth obtained by cultivating a neamine-negative mutant of the butirosin-producing organism bacillus circulans in the medium supplemented with 6'-n-methylgentamine c1a. these antibiotics were characterized and elucidated as 3', 4'-dideoxy-6'-n-methylbutirosins a and b (dmb-a & dmb-b), by chemical and spectroscopic studies. dmb-a and dmb-b exhibited broad-spectrum antibacterial activities with in vitro potency similar to or slight ... | 1978 | 81823 |
| mutational biosynthesis of butirosin analogs. i. conversion of neamine analogs into butirosin analogs by mutants of bacillus circulans. | by n-methyl-n'-nitro-n-nitrosoguanidine treatment, neamine-negative mutants which required neamine for biosynthesis of butirosins were obtained from a butirosin-producing organism bacillus circulans. these mutants also produced butirosins from paromamine and could be divided into two types i and ii. mutants of type i could not produce butirosins from 2-deoxystreptamine, whereas those of type ii could. two typical mutants mcrl 5003 (type i) and mcrl 5004 (type ii) could produce butirosin analogs, ... | 1978 | 81822 |
| aminoglycoside 3'-phosphotransferase in bacillus circulans producing butirosins. | 1977 | 69624 | |
| antibiotics derived from a mutant of bacillus circulans. | a mutant of bacillus circulans, which produces butirosins only when 2-deoxystreptamine is added to the fermentation medium, was employed in the biosynthesis of antibiotics containing modified aminocyclitols. the blocked mutant converted 2,5-dideoxystreptamine into 5-deoxybutirosamine. streptamine was incorporated into a complex differing from butirosin by an additional hydroryl at c-2. | 1976 | 60328 |
| fine structure and distribution of extracellular polymer surrounding selected aerobic bacteria. | the structure and distribution of extracellular polymer surrounding bacillus circulans, diplococcus (streptococcus) pneumoniae, streptococcus salivarius, staphylococcus aureus, klebsiella pneumoniae, pseudomonas aeruginosa, herella vaginacola (acinetobacter calcoaceticus), and agrobacterium tumefaciens were studied by electron microscopy. a modified ruthenium red staining procedure was used to examine the fine structure of capsule and slime. freeze-etching and critical-point drying were used to ... | 1975 | 46774 |
| simple method for the isolation of astaxanthin from the basidiomycetous yeast phaffia rhodozyma. | a method is described for the quantitative and, possibly, large-scale extraction of astaxanthin from the yeast phaffia rhodozyma. the method utilizes extracellular enzymes produced by the bacterium bacillus circulans wl-12, which partially digests the yeast cell wall and renders the carotenoid pigments extractable by acetone or ethanol. complete recovery of astaxanthin from heat-killed p. rhodozyma cells was obtained after growing b. circulans wl-12 on these yeast cells for 26 h and then extract ... | 1978 | 28079 |
| lysis of yeast cell walls. lytic beta-(1 leads to 3)-glucanases from bacillus circulans wl-12. | bacillus circulans wl-12 when grown in a mineral medium with yeast cell walls or yeast glucan as the soli carbon source, produced five beta-glucanases. two beta-(1 leads to 3)-glucanases (i and ii), which are lytic to yeast cell walls, were isolated from the culture liquid by batch adsorption on yeast glucan, and separated by chromatography on hydroxylapatite. lytic beta-(1 leads to 3)-glucanase i was further purified by carboxymethylcellulose chromatography. the specific activity of lytic beta- ... | 1976 | 4310 |
| lysis of yeast cell walls. lytic beta-(1 leads to 6)-glucanase from bacillus circulans wl-12. | when grown in a mineral medium with yeast cell walls or yeast glucan as the sole carbon source, bacillus circulans wl-12 produces wall-lytic enzymes in addition to non-lytic beta-(1 leads to 3) and beta-(1 leads to 6)-glucananases. the lytic enzymes were isolated from the culture liquid by adsorption on insoluble yeast glucan in batch operation. after digestion of the glucan, the mixture of enzymes was chromatographed on hydroxylapatite on which the lytic activity could be resolved into one lyti ... | 1976 | 4309 |