Publications

transgalactosylation and hydrolytic activities of commercial preparations of β-galactosidase for the synthesis of prebiotic carbohydrates.	β-galactosidases exhibit both hydrolytic and transgalactosylation activities; the former has been used traditionally for the production of delactosed milk and dairies, while the latter is being increasingly used for the synthesis of lactose-derived oligosaccharides: balance between both activities was highly dependent on the enzyme origin: β-galactosidases from aspegillus oryzae and bacillus circulans exhibited high transgalactosylation activity, while those from one from kluyveromyces exhibited ...	2015	25659627
purification and characterization of organic solvent stable serine alkaline protease from newly isolated bacillus circulans m34.	a protease from newly isolated bacillus circulans m34 was purified by q-sepharose anion exchange chromatography and sepharose-bacitracin affinity chromatography followed by (nh4)2so4 precipitation. the molecular mass of the purified enzyme was determined using sds-page. the optimum ph and temperature for protease activity were 11 and 50°c, respectively. the effect of various metal ions on protease activity was investigated. alkaline protease from bacillus circulans m34 wase activated by zn(2+), ...	2015	25677873
molecular docking and site-directed mutagenesis of a bacillus thuringiensis chitinase to improve chitinolytic, synergistic lepidopteran-larvicidal and nematicidal activities.	bacterial chitinases are useful in the biocontrol of agriculturally important pests and fungal pathogens. however, the utility of naturally occurring bacterial chitinases is often limited by their low enzyme activity. in this study, we constructed mutants of a bacillus thuringiensis chitinase with enhanced activity based on homology modeling, molecular docking, and the site-directed mutagenesis of target residues to modify spatial positions, steric hindrances, or hydrophilicity/hydrophobicity. w ...	2015	25678849
diversity of bacteria nesting the plant cover of north sinai deserts, egypt.	north sinai deserts were surveyed for the predominant plant cover and for the culturable bacteria nesting their roots and shoots. among 43 plant species reported, 13 are perennial (e.g. fagonia spp., pancratium spp.) and 30 annuals (e.g. bromus spp., erodium spp.). eleven species possessed rhizo-sheath, e.g. cyperus capitatus, panicum turgidum and trisetaria koelerioides. microbiological analyses demonstrated: the great diversity and richness of associated culturable bacteria, in particular nitr ...	2012	25685397
characterization of deep sea fish gut bacteria with antagonistic potential, from centroscyllium fabricii (deep sea shark).	the bacterial isolates from centroscyllium fabricii (deep sea shark) gut were screened for antagonistic activity by cross-streak method and disc diffusion assay. this study focuses on strain btss-3, which showed antimicrobial activity against pathogenic bacteria including salmonella typhimurium, proteus vulgaris, clostridium perfringens, staphylococcus aureus, bacillus cereus, bacillus circulans, bacillus macerans and bacillus pumilus. btss3 was subjected to phenotypic characterization using bio ...	2015	25740801
mutations at calcium binding site iii in cyclodextrin glycosyltransferase improve β-cyclodextrin specificity.	cyclodextrin glycosyltransferases (cgtases, ec 2.4.1.19) are industrially important enzymes that produce cyclodextrins from starch by intramolecular transglycosylation. in this study, the effects of amino acid residue at position 315 in calcium binding site iii (caiii) on product specificity of cgtase were investigated by replacing ala315 in the cgtase from bacillus circulans stb01 with arginine, aspartic acid, threonine, leucine and valine. the cgt gene, which encodes this enzyme, was expressed ...	2015	25748847
recombinant, truncated b. circulans keratanase-ii: description and characterisation of a novel enzyme for use in measuring urinary keratan sulphate levels via lc-ms/ms in morquio a syndrome.	morquio a syndrome (mucopolysaccharidosis iva; mps iva) is an autosomal recessive lysosomal storage disorder caused by deficient n-acetylgalactosamine-6-sulphatase (galns) activity. early and accurate diagnosis of this condition is critical for improved patient outcomes, particularly as enzyme replacement therapy has recently become available. an lc-ms/ms assay utilising keratan sulphate (ks) disaccharides derived from keratanase-ii digestion provides a sensitive and specific means for quantitat ...	2015	25866399
crystal structure of β-galactosidase from bacillus circulans atcc 31382 (bgad) and the construction of the thermophilic mutants.	β-galactosidase (ec 3.2.1.23) from bacillus circulans atcc 31382, designated bgad, exhibits high transglycosylation activity to produce galacto-oligosaccharides. bgad has been speculated to have a multiple domain architecture including a f5/8-type c domain or a discoidin domain in the c-terminal peptide region from amino acid sequence analysis. here, we solved the first crystal structure of the c-terminal deletion mutant bgad-d, consisting of sugar binding, glyco_hydro, catalytic and bacterial i ...	2015	25879162
production of alkaline protease by solvent-tolerant alkaliphilic bacillus circulans mtcc 7942 isolated from hydrocarbon contaminated habitat: process parameters optimization.	in the present investigation, a newly isolated organic solvent-tolerant and alkaliphilic bacterial strain was reported from a hydrocarbon (gasoline and diesel) contaminated soil collected from the petrol station, shirpur (india). the strain was identified as bacillus circulans mtcc 7942, based on phenotype, biochemical, and phylogenetic analysis of 16s rrna gene sequence. the capability of bacillus circulans to secrete an extracellular, thermostable, alkaline protease and grow in the presence of ...	2013	25937965
comparative study of the fungicide benomyl toxicity on some plant growth promoting bacteria and some fungi in pure cultures.	six laboratory experiments were carried out to investigate the effect of the fungicide benomyl on pure cultures of some plant growth promoting bacteria (pgpb) and some fungi. the highest ld50 was recorded for bacillus circulans and proved to be the most resistant to the fungicide, followed by azospirillum braziliense, while penicillium sp. was the most affected microorganism. ld50 values for the affected microorganisms were in 21-240 orders of magnitude lower in comparison with the ld50 value fo ...	2014	26038670
relationship between sol-gel conditions and enzyme stability: a case study with β-galactosidase/silica biocatalyst for whey hydrolysis.	the sol-gel process has been very useful for preparing active and stable biocatalysts, with the possibility of being reused. especially those based on silica are well known. however, the study of the enzyme behavior during this process is not well understood until now and more, if the surfactant is involved in the synthesis mixture. this work is devoted to the encapsulation of β-galactosidase from bacillus circulans in silica by sol-gel process, assisted by non-ionic triton x-100 surfactant. the ...	2015	26313518
monobody-mediated alteration of enzyme specificity.	current methods for engineering enzymes modify enzymes themselves and require a detailed mechanistic understanding or a high-throughput assay. here, we describe a new approach where catalytic properties are modulated with synthetic binding proteins, termed monobodies, directed to an unmodified enzyme. using the example of a β-galactosidase from bacillus circulans, we efficiently identified monobodies that restricted its substrates for its transgalactosylation reaction and selectively enhanced th ...	2015	26322825
de novo biosynthesis of β-valienamine in engineered streptomyces hygroscopicus 5008.	the c7n aminocyclitol β-valienamine is a lead compound for the development of new biologically active β-glycosidase inhibitors as chemical chaperone therapeutic agents for lysosomal storage diseases. its chemical synthesis is challenging due to the presence of multichiral centers in the structure. herein, we took advantage of a heterogeneous aminotransferase with stereospecificity and designed a novel pathway for producing β-valienamine in streptomyces hygroscopicus 5008, a validamycin producer. ...	2016	26436873
association of bacillus circulans with non-diabetic foot infection in bangladeshi patient.		2015	26470981
asp577 mutations enhance the catalytic efficiency of cyclodextrin glycosyltransferase from bacillus circulans.	the amino acid residue asp 577 is located in calcium-binding site iii (caiii) of the cyclodextrin glycosyltransferase (ec 2.4.1.19, cgtase) from bacillus circulans stb01. in the present study, the effects of replacing asp577 with glycine, alanine, valine, leucine, and isoleucine on the catalytic efficiency of this cgtase were investigated. two of these replacements, d577g and d577a, increased the β-cyclization activity of cgtase. kinetic studies showed that the km values of d577g and d577a were ...	2016	26608005
larvicidal activity of bacillus circulans against the gastrointestinal nematode haemonchus contortus in sheep.	efficient control of gastrointestinal parasites is necessary in sheep breeding. however, the available chemically based anthelmintics are becoming less effective due to the development of parasite resistance. an alternative to this problem is biological control. in the present study, we tested the larvicidal effect of bacillus circulans by administering a spore suspension (2 × 109 colony forming units/ml) orally to lambs naturally infected with haemonchus contortus. the number of faecal larvae w ...	2016	26693886
characterization of mannanase from bacillus circulans nt 6.7 and its application in mannooligosaccharides preparation as prebiotic.	this study focused on the characterization of mannanase from bacillus circulans nt 6.7 for mannooligosaccharides (mos) production. the enzyme from b. circulans nt 6.7 was produced using defatted copra meal as a carbon source. the mannanase was purified by ultrafiltration and column chromatography of q-sepharose. the purified protein (m1) was a dimeric protein with a 40 kda subunit. the purified m1 exhibited optimum ph and temperature at ph 6.0 and 60 °c, respectively. it was activated by mn(2+,) ...	2015	26697281
α-1,3-glucanase: present situation and prospect of research.	α-1,3-glucanases hydrolyze α-1,3-glucan which is an insoluble linear α-1,3-linked homopolymer of glucose and these enzymes are classified into two families of glycoside hydrolases on the basis of amino acid sequence similarity; type-71 α-1,3-glucanases found in fungi and type-87 enzymes in bacteria. α-1,3-glucan (also called 'mutan') is a major component of dental plaque formed by oral streptococci and has important physiological roles in various fungal species, including as a component of cell ...	2016	26748807
thermostable β-galactosidases for the synthesis of human milk oligosaccharides.	human milk oligosaccharides (hmos) designate a unique family of bioactive lactose-based molecules present in human breast milk. using lactose as a cheap donor, some β-galactosidases (ec 3.2.1.23) can catalyze transgalactosylation to form the human milk oligosaccharide lacto-n-neotetraose (lnnt; gal-β(1,4)-glcnac-β(1,3)-gal-β(1,4)-glc). in order to reduce reaction times and be able to work at temperatures, which are less welcoming to microbial growth, the current study investigates the possibilit ...	2016	26802542
a hybrid strategy using global analysis of oxidized fatty acids and bioconversion by bacillus circulans.	targeted oxidized fatty acid analysis has been widely used to understand the roles of fatty acids in the development of diseases. however, because of the extensive structural diversity of fatty acids, it is considered that unknown lipid metabolites will remain undetected. here, to discover and identify unknown lipid metabolites in biological samples, a global analytical system and a method of synthesizing lipid standards were investigated.	2016	26864527
bacillus circulans exopolysaccharide: production, characterization and bioactivities.	a bacterium with the ability to produce appreciable amount of exopolysaccharide was isolated from slimy layer of coconut. 16s rdna analysis identified the organism as bacillus circulans. eps production was observed at all stages of culture growth and reached maximum of 0.065mg/ml by 96h, which on further incubation started to decrease. response surface methodology using box behnken design has shown the influence of sucrose which was found to be directly proportional to exopolysaccharide producti ...	2016	26902891
[anchorage of cyclodextrin glycosyltransferase on outer membrane of saccharomyces cerevisiae to produce 2-o-α-d-glucopyranosyl-l-ascorbic acid].	displaying cyclodextrin glycosyltransferase ( cgtase) from bacillus circulans 251 on the cell surface of saccharomyces cerevisiae to improve 2-o-α-d-glucopyranosyl-l-ascorbic acid (aa-2g) production.	2015	26939459
comparative structural characterization of 7 commercial galacto-oligosaccharide (gos) products.	many β-galactosidase enzymes convert lactose into a mixture of galacto-oligosaccharides (gos) when incubated under the right conditions. recently, the composition of commercial vivinal gos produced by bacillus circulans β-galactosidase was studied in much detail in another study by van leeuwen et al. as a spin-off of this study, we used the developed analytical strategy for the evaluation of 6 anonymous commercial gos products, in comparison with vivinal gos. these gos products were first subjec ...	2016	27035911
bacillus oryzisoli sp. nov., isolated from rice rhizosphere.	the taxonomy of strain 1ds3-10t, a gram-staining-positive, endospore-forming bacterium isolated from rice rhizosphere, was investigated using a polyphasic approach. phylogenetic analysis based on 16s rrna gene sequences demonstrated that the novel strain was grouped with established members of the genus bacillus and appeared to be closely related to the type strains bacillus benzoevorans dsm 5391t (97.9 %), bacillus circulans dsm 11t (97.7 %), bacillus novalis jcm 21709t (97.3 %), bacillus soli ...	2016	27265352
properties of a highly viscous polysaccharide produced by a bacillus strain isolated from soil.	chemical and theological properties of a highly viscous and acidic polysaccharide produced by a soil bacterium identified as bacillus circulans are described. the molecular weight of the native polysaccharide was about 116 × 10(4) by gel filtration with hplc. the molar ratio of d-galactose, n-mannose, and l-rhamnose contained in the polysaccharide as neutral sugar components was 1.3:1:2. the viscosity of 1% solution of the polysaccharide was about 5000 cp, which was higher than that of guar gum, ...	1992	27280661
purification and some properties of chitinase b1 from bacillus circulans wl-12.		1992	27280674
production of a novel extracellular polysaccharide by a bacillus strain isolated from soil.	a bacterium that was isolated from soil and identified as bacillus circulans was found to produce a highly viscous extracellular polysaccharide when it was grown aerobically in a medium containing glucose as a sole source of carbon. the product was characterized by tlc and gc analyses as a novel heteropolysaccharide consisted of rhamnose, mannose, galactose, and mannuronic acid as sugar components. a maximal yield of polysaccharide reached about 2 g/liter by jar-fermentor culture at 30°c for 48 ...	1992	27280807
purification and some properties of p-nitrophenyl-β-d-glucoside-hydrolyzing enzymes in culture filtrate of bacillus circulans ka-304 grown on cell-wall preparation of schizophyllum commune.	hydrolyzing activities toward p-nitrophenyl (p-np)-β-d-glucoside and laminarin in a culture filtrate of bacillus circulans ka-304, which has been observed to form protoplasts from schizophyllum commune mycelia, increased when the bacterium was grown on a cell-wall preparation (cwp) of s. commune or laminarin as a carbon source. an analysis of the filtrate with the cwp suggested occurrence of two major p-np-β-d-glucoside-hydrolyzing enzymes (β-d-glucosidases i and ii) and a laminarin-hydrolyzing ...	1998	27393352
characterization of the immobilized β-galactosidase c from bacillus circulans and the production of β(1→3)-linked disaccharides.	a recombinant β-galactosidase, which was obtained from the β-galactosidase c gene of bacillus circulans and cleaves the non-reducing end galactosyl residue of β(1→3)-linkages selectively, was immobilized using cnbr-sepharose. although the effect of ph was not changed by the immobilization, the thermostability and stability in the presence of dmf were increased. optimization of the transglycosylation using para-nitrophenyl β-d-galactopyranoside as a donor and benzyl-α-d-n-acetylgalactosaminide as ...	1998	27396997
a novel chemoenzymatic synthesis of sulfated type 2 tumor-associated carbohydrate antigens by transglycosylation of sulfated lewis x oxazoline catalyzed by keratanase ii.	sulfated type 2 carbohydrate chains are known tumor-associated carbohydrate antigens (tacas). many reports on cancer vaccines employing tacas as specific antigens have been published, but structurally specified sulfated tacas have not been used because of the low natural abundance and difficulties in chemical synthesis. we demonstrate for the first time the synthesis of the sulfated type 2 tacas with an l-fucose branch by keratanase-ii-catalyzed transglycosylation of the sulfated lewis x (galβ(1 ...	2016	27400395
rationalising pka shifts in bacillus circulans xylanase with computational studies.	bacillus circulans xylanase (bcx), a family 11 glycoside hydrolase, catalyses the hydrolysis of xylose polymers with a net retention of stereochemistry. glu172 in bcx is believed to act as a general acid by protonating the aglycone during glycosylation, and then as a general base to facilitate the deglycosylation step. the key to the dual role of this general acid/base lies in its protonation states, which depend on its intrinsic pka value and the specific environment which it resides within. to ...	2016	27485091
synthesis, characterization and biological studies of schiff bases derived from heterocyclic moiety.	some new schiff bases (h1-h7) have been synthesized by the condensation of 2-aminophenol, 2-amino-4-nitrophenol, 2-amino-4-methylphenol, 2-amino benzimidazole with thiophene-2-carboxaldehyde and pyrrole-2-carboxaldehyde. the structures of newly synthesized compounds were characterized by elemental analysis, ft-ir, (1)h nmr, uv-vis, and single crystal x-ray crystallography. the in vitro antibacterial activity of the synthesized compounds has been tested against salmonella typhi, bacillus coagulan ...	2017	27894775
structure-activity relationships on the study of β-galactosidase folding/unfolding due to interactions with immobilization additives: triton x-100 and ethanol.	improving the enzyme stability is a challenge for allowing their practical application. the surfactants are stabilizing agents, however, there are still questions about their influence on enzyme properties. the structure-activity/stability relationship for β-galactosidase from bacillus circulans is studied here by circular dichroism and activity measurements, as a function of temperature and ph. the tendency of preserving the β-sheet and α-helix structures at temperatures below 65°c and differen ...	2017	27965126
[activation of biosynthesis of guanyl-specific ribonuclease secreted by bacillus circulans under salt stress].	the gene transcription of guanyl-specific ribonucleases (rnases), which provide available phosphate to cells of bacillus, is controlled by the signal transduction system phop-phor. however, the biosynthesis of b. circulans rnase does not depend on the signal-transduction regulatory proteins of pho regulon. it has been found that raising the salt molar concentration in culture medium increases the level of extracellular guanyl-specific ribonuclease bci synthesized by b. circulans. sequences homol ...	2017	28064315
engineering of the bacillus circulans β-galactosidase product specificity.	microbial β-galactosidase enzymes are widely used as biocatalysts in industry to produce prebiotic galactooligosaccharides (gos) from lactose. gos mixtures are used as beneficial additives in infant formula to mimic the prebiotic effects of human milk oligosaccharides (hmos). the structural variety in gos mixtures is significantly lower than in hmos. since this structural complexity is considered as the basis for the multiple biological functions of hmos, it is important to broaden the variety o ...	2017	28092444
stabilization of bacillus circulans xylanase by combinatorial insertional fusion to a thermophilic host protein.	high thermostability of an enzyme is critical for its industrial application. while many engineering approaches such as mutagenesis have enhanced enzyme thermostability, they often suffer from reduced enzymatic activity. a thermally stabilized enzyme with unchanged amino acids is preferable for subsequent functional evolution necessary to address other important industrial needs. in the research presented here, we applied insertional fusion to a thermophilic maltodextrin-binding protein from pyr ...	2017	28100651
synthesis of an allergy inducing tetrasaccharide "4p-x".	4p-x (β-d-galactopyranosyl-(1 → 4)-β-d-galactopyranosyl-(1 → 6)-[β-d-galactopyranosyl-(1 → 4)]-β-d-glucopyranose) is included in galacto-oligosaccharides (goss) produced by β-galactosidase derived from bacillus circulans. 4p-x has been known to induce particularly strong allergies. high purity 4p-x is essential for use as a standard to quantify the amount of 4p-x in goss; however, the isolation of high purity 4p-x has never been reported. in this study, we achieved the synthesis of 4p-x by a com ...	2017	28107656
reaction kinetics and galactooligosaccharide product profiles of the β-galactosidases from bacillus circulans, kluyveromyces lactis and aspergillus oryzae.	β-galactosidase enzymes are used in the dairy industry to convert lactose into galactooligosaccharides (gos) that are added to infant formula to mimic the molecular sizes and prebiotic functions of human milk oligosaccharides. here we report a detailed analysis of the clearly different gos profiles of the commercial β-galactosidases from bacillus circulans, kluyveromyces lactis and aspergillus oryzae. also the gos yields of these enzymes differed, varying from 48.3% (b. circulans) to 34.9% (k. l ...	2017	28193420
position 228 in paenibacillus macerans cyclodextrin glycosyltransferase is critical for 2-o-d-glucopyranosyl-l-ascorbic acid synthesis.	the markedly stable l-ascorbic acid (l-aa) derivative 2-o-d-glucopyranosyl-l-ascorbic acid (aa-2g) has been widely used in the fields of food, medicine, cosmetics, and husbandry. cyclodextrin glycosyltransferase (cgtase) is considered suitable for the large-scale production of aa-2g. in this work, paenibacillus macerans cgtase was used to produce aa-2g and the production was 13.5g/l. an amino-acid sequence alignment of α-, β-, and α⁄β-cgtase indicated that the phe at position 228 of p. macerans ...	2017	28219734
immobilized trienzymatic system with enhanced stabilization for the biotransformation of lactose.	the use of ketohexose isomerases is a powerful tool in lactose whey processing, but these enzymes can be very sensitive and expensive. development of immobilized/stabilized biocatalysts could be a further option to improve the process. in this work, β-galactosidase from bacillus circulans, l-arabinose (d-galactose) isomerase from enterococcus faecium, and d-xylose (d-glucose) isomerase from streptomyces rubiginosus were immobilized individually onto eupergit c and eupergit c 250 l. immobilized a ...	2017	28241449
simultaneous decolorization of sulfonated azo dyes and reduction of hexavalent chromium under high salt condition by a newly isolated salt-tolerant strain bacillus circulans bwl1061.	the co-existence of dyes, cr(vi) and high concentration of salt in dyeing wastewater causes serious and complex environmental problems. in this study, a salt-tolerant strain bacillus circulans bwl1061 was reported to simultaneously remove 50mg/l methyl orange and 50mg/l cr(vi) under the anaerobic condition with 60g/l nacl. during the decolorization process, the cr(vi) reduction occurred preferentially over the dye decolorization due to the dominate utilization of electron by cr(vi). the analysis ...	2017	28284151
an efficient process for obtaining prebiotic oligosaccharides derived from lactulose using isomerized and purified whey permeate.	one of the most promising uses of whey permeate (wp) is the synthesis of prebiotic oligosaccharides. herein, commercial wp was submitted to chemical isomerization catalysed by sodium borate at an alkaline ph and subsequent purification using anion-exchange resins to remove boron. subsequently, purified mixtures were used to synthesize prebiotic oligosaccharides using β-galactosidase from bacillus circulans.	2017	28417455
fusion of a family 20 carbohydrate-binding module (cbm20) with cyclodextrin glycosyltransferase of geobacillus sp. chb1 improves catalytic efficiency.	cyclodextrin glycosyltransferase (cgtase) is an important industrial enzyme for production of cyclodextrins (cds) from starch by intramolecular transglycosylation. cgtase consists of five domains labeled a to e. for optimizing catalytic activity of cgtase, cgtase of geobacillus sp. was fused with the family 20 carbohydrate-binding module (cbm) of the bacillus circulans strain 251 cgtase. the cbmbc251 that has a low binding free energy with maltohexaose, was selected by in silico design. then the ...	2017	28422446
refolding the unfoldable: a systematic approach for renaturation of bacillus circulans xylanase.	xylanases are important polysaccharide-cleaving catalysts for the pulp and paper, animal feeds and biofuels industries. they have also proved to be valuable model systems for understanding enzymatic catalysis, with one of the best studied being the gh11 xylanase from bacillus circulans (bcx). however, proteins from this class are very recalcitrant to refolding in vitro. this both limits their high level expression in heterologous hosts, and prevents experimental approaches, such as peptide ligat ...	2017	28466501
complete genome sequence of the bacterium bacillus circulans jordan strain 32352.	here, we report the complete genome sequence for the bacillus circulans jordan strain 32352. this species is a soil dwelling bacterium that expresses glycosyl hydrolase enzymes degrading pneumococcal capsular polysaccharides.	2017	28495770
biochemical characterization of the functional roles of residues in the active site of the β-galactosidase from bacillus circulans atcc 31382.	the β-galactosidase enzyme from bacillus circulans atcc 31382 bgad is widely used in the food industry to produce prebiotic galactooligosaccharides (gos). recently, the crystal structure of a c-terminally truncated version of the enzyme (bgad-d) has been elucidated. the roles of active site amino acid residues in β-galactosidase enzyme reaction and product specificity have remained unknown. on the basis of a structural alignment of the β-galactosidase enzymes bgad-d from b. circulans and bgaa fr ...	2017	28538097
synthesis and characterization of novel astragalin galactosides using β-galactosidase from bacillus circulans.	astragalin (kaempferol-3-o-β-d-glucopyranoside, ast) is a kind of flavonoid known to have anti-oxidant, anti-hiv, anti-allergic, and anti-inflammatory effects. it has low solubility in water. in this study, novel astragalin galactosides (ast-gals) were synthesized using β-galactosidase from bacillus circulans and reaction conditions were optimized to increase the conversion yield of astragallin. purified ast-gal1 (11.6% of ast used, w/w) and ast-gal2 (6.7% of ast used, w/w) were obtained by medi ...	2017	28554386
the structure of rbmb from streptomyces ribosidificus, an aminotransferase involved in the biosynthesis of ribostamycin.	aminoglycoside antibiotics represent a classical group of antimicrobials first discovered in the 1940s. due to their ototoxic and nephrotoxic side effects, they are typically only used against gram negative bacteria which have become resistant to other therapeutics. one family of aminoglycosides includes such compounds as butirosin, ribostamycin, neomycin, and kanamycin, amongst others. the common theme in these antibiotics is that they are constructed around a chemically stable aminocyclitol un ...	2017	28685903
biosurfactant production from pseudomonas taiwanensis l1011 and its application in accelerating the chemical and biological decolorization of azo dyes.	dye dispersion and the interaction efficiency between azoreductases and dye molecules are rate-limiting steps for the decolorization of azo dyes. in this study, a biosurfactant-producing strain, pseudomonas taiwanensis l1011, was isolated from crude oil. to increase the yield of the biosurfactant bs-l1011 from p. taiwanensis l1011, culture conditions were optimized including temperature, initial ph, carbon source, nitrogen source and c/n ratio. a maximum yield of 1.12g/l of bs-l1011 was obtained ...	2017	28689070
secretory expression of β-mannanase from bacillus circulans nt 6.7 in lactobacillus plantarum.	the β-mannanase gene of bacillus circulans nt 6.7 was successfully cloned in lactobacillus plantarum wcfs1 using the psip403 expression vector and secreted to the supernatant rather than accumulated in the cells. the highest activity was achieved by controlling the ph at 6 during cultivation. maximum mannanase activities detected in the supernatant and cell-free extract of 200 ml mrs broth were 8.2 and 0.86 u/ml, respectively. enzyme activity in the supernatant increased to 27 u/ml by fermentati ...	2017	28712957
construction and functional characterization of truncated versions of recombinant keratanase ii from bacillus circulans.	there is a need for degradative enzymes in the study of glycosaminoglycans. many of these enzymes are currently available either in their natural or recombinant forms. unfortunately, progress in structure-activity studies of keratan sulfate (ks) have been impeded by the lack of a commercially available endo-β-n-acetylglucosaminidase, keratantase ii. the current study uses a recently published sequence of a highly thermostable keratanase ii identified in bacillus circulans to clone and express a ...	2017	28752383
an atypical interaction explains the high-affinity of a non-hydrolyzable s-linked 1,6-α-mannanase inhibitor.	the non-hydrolyzable s-linked azasugars, 1,6-α-mannosylthio- and 1,6-α-mannobiosylthioisofagomine, were synthesized and shown to bind with high affinity to a family 76 endo-1,6-α-mannanase from bacillus circulans. x-ray crystallography showed an atypical interaction of the isofagomine nitrogen with the catalytic acid/base. molecular dynamics simulations reveal that the atypical binding results from sulfur perturbing the most stable form away from the nucleophile interaction preferred for the o-l ...	2017	28766587
a chitin film containing basic fibroblast growth factor with a chitin-binding domain as wound dressings.	basic fibroblast growth factor (bfgf) can stimulate wound healing. however, consistent delivery of bfgf has many disadvantages. to decrease their instability and diffusible, we introduced chitin-binding domain (chtbd) into bfgf. two expression plasimids were constructed. the first one (named bfgf) contained bfgf (154 amino acids), the second (named chtbd-bfgf) contained bfgf and the chtbd (54 amino acids). chtbd was derived from chitinase a1 (chia1) of bacillus circulans wl-12. the recombinant p ...	2017	28821125