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cloning and characterization of a potentially protective chitinase-like recombinant antigen from wuchereria bancrofti.while there is no direct evidence demonstrating the existence of protective immunity to wuchereria bancrofti infection in humans, the presence of individuals, in populations in areas where infection is endemic, with no clinical evidence of past or current infection despite appreciable exposure to the infective larvae, suggests that protective immunity to filarial parasites may occur naturally. earlier work indicated that such putatively immune individuals generated antibodies to a 43-kda antigen ...19948168956
[cloning of the gene for extracellular bacillus circulans rnaase].the gene for extracellular low molecular weight ribonuclease of bacillus circulans bcf 247 was cloned. the strain was isolated from permafrost deposits of the kolyma lowland. the gene for the ribonuclease from bacillus intermedius (binase) was used as a specific probe. the cloning succeeded only in the e. coli strain producing the inhibitor of ribonuclease form bacillus amyloliquefaciens. selected clones secreted the active ribonuclease into the growth media. deletion derivatives of the parental ...19948183279
isolation and characterization of soybean waste-degrading microorganisms and analysis of fertilizer effects of the degraded products.two microorganisms which could degrade soybean lees efficiently were isolated and identified as bacillus circulans and b. stearothermophilus. these two strains secreted thermostable proteases into the medium and could digest soybean lees rapidly and completely at 50 degrees c. initially, the soybean lees were degraded to proteins in approximately 20 h by these two strains, after which time the concentrations of peptides in the medium gradually increased. the degraded products from soybean lees c ...19948117080
isolation and sequence of an endochitinase-encoding gene from a cdna library of trichoderma harzianum.there are no reports of gene sequences coding for extracellular chitinolytic enzymes from filamentous fungi, even though these enzymes are considered critical to the biological control of plant pathogenic fungi. the purpose of this paper was to report the isolation of a gene (then-42) encoding endochitinase (ech) from trichoderma harzianum strain p1, describe its sequence, and to determine whether it was related to genes coding for enzymes with similar functions from prokaryotic or other eukaryo ...19948125293
purification and properties of β-amylase from bacillus circulans s31.a thermotolerant β-amylase was purified from bacillus circulans s31 isolated from soil in hong kong. the purified enzyme has an m r of 64 kda and was stable at 50°c and ph 7.0 for 30 min. its k m for starch was 0.9 mg/ml with a v max of 0.3 mg/min. it was not activated by any metal ion although sulphydrys reagents were inhibitory.199424421144
primary structure and catalytic properties of extracellular ribonuclease of bacillus circulans.a complete amino acid sequence of extracellular bacillus circulans rnase was established and compared with a structure of b. amyloliquefaciens rnase. gln15, gly65 and gln104 in b. amyloliquefaciens rnase were found to be replaced by leu, ala and lys, respectively, in b. circulans rnase. catalytic properties of b. circulans rnase were studied.19938224254
[primary structure and catalytic properties of extracellular ribonuclease from bacillus circulans].a comparative research of individual peptide structures obtained after hydrolysing of bacillus circulans and b. amyloliquefaciens rnases by the glu-specific staphylococcal protease was carried out by means of mass-spectrometry and edman degradation methods. a complete amino acid sequence of b. circulans rnase was determined. gln15, gly65 and gln104 residues in b. amyloliquefaciens rnase were found to be substituted by leu, ala and lys residues in b. circulans rnase, respectively. catalytic prope ...19938285919
characteristics of an exochitinase from streptomyces olivaceoviridis, its corresponding gene, putative protein domains and relationship to other chitinases.streptomyces olivaceoviridis efficiently degrades chitin. shotgun cloning of partially sau3a-cleaved dna using the multicopy vector pij702 and streptomyces lividans 66 as host resulted in the identification of the plasmid pchi o1 which harbours an insert of 4.6 kb. in the presence of chitin as sole carbon source, transformants of s. lividans 66 carrying pchi o1 or its derivatives with smaller inserts overproduced an exochitinase which was purified to homogeneity. the chitin-inducible enzyme with ...19938319677
a convenient synthesis of beta-d-galactosyl disaccharide derivatives using the beta-d-galactosidase from bacillus circulans.beta-d-gal-(1-->4)-beta-d-glcnac-oc6h4no2-p (p-nitrophenyl n-acetyl-beta-lactosaminide) and beta-d-gal-(1-->6)-beta-d-glcnac-oc6h4no2-p (p-nitrophenyl n-acetyl-beta-isolactosaminide) were regioselectively synthesized from lactose and p-nitrophenyl 2-acetamido-2-deoxy-glucopyranoside, employing transglycosylation by the beta-d-galactosidase from bacillus circulans and by controlling the concentration of organic solvent in the reaction system. the (1-->4)-linked disaccharide was formed exclusively ...19938348555
preparation, isolation, and characterization of novel heterogeneous branched cyclomalto-oligosaccharides having beta-d-galactosyl residue(s) on the side chain.transgalactosylated products of branched cyclodextrins (glucosyl-alpha cd, -beta cd, -gamma cd, and maltosyl-alpha cd, -beta cd, -gamma cd) were synthesized by beta-d-galactosidases from bacillus circulans and penicillium multicolor using lactose as a donor substrate and branched cds as acceptors. eighteen beta-d-galactosylated branched cds were isolated and purified by hplc. their structures were elucidated by fabms and 13c nmr spectroscopies, and methylation analysis. the chromatographic behav ...19938431940
purification and properties of a thermostable chitinase from streptomyces thermoviolaceus opc-520.a chitinase was purified from the culture filtrate of streptomyces thermoviolaceus opc-520. the enzyme showed a high optimum temperature (70 to 80 degrees c), a high optimum ph level (8.0 to 10.0), and heat stability. this enzyme showed high sequence homology with chitinases from serratia marcescens qmb1466 and bacillus circulans wl-12.19938434929
purification, characterization, gene cloning, and sequencing of a new beta-glucosidase from bacillus circulans subsp. alkalophilus.an intracellular beta-glucosidase was purified from cell extracts of bacillus circulans subsp. alkalophilus by nad affinity and high-performance anion-exchange chromatographies. the enzyme was active against a wide range of aryl-beta-glucosides and beta-linked disaccharides. the structural gene for beta-glucosidase was cloned in escherichia coli. the beta-glucosidase gene consisted of an open reading frame of 1,350 bp encoding a protein of 450 amino acids with a calculated m(r) of 51,303. the en ...19938481013
antimicrobial activity determined in strains of bacillus circulans cluster.wild-type strains of the genus bacillus were screened for antimicrobial activity. two strains exhibited antimicrobial activity against micrococcus luteus and were identified as bacillus polymyxa mir-23 and bacillus circulans mir-13. bacillus polymyxa mir-23 was active against escherichia coli, pseudomonas aeruginosa and aspergillus niger, whereas bacillus circulans mir-13 did not show activity against these microorganisms. bacillus subtilis atcc 6633 and b. polymyxa atcc 10401 were used as stand ...19938500778
alpha-amylase production in lactose medium by bacillus circulans acb.alpha-amylase production by bacillus circulans acb was studied in various cultural conditions. during nutrient optimisation, it was found that 2% lactose can be utilized by the strain as source of carbon providing better growth and enzyme yields than starch. ammonium sulfate of the basal medium can be replaced by ammonium nitrate for better growth and alpha-amylase activity. the strain demonstrated significant enhancement in alpha-amylase production when grown at ph 6.6.19938172692
cloning of the beta-amylase gene from bacillus cereus and characteristics of the primary structure of the enzyme.the gene encoding the beta-amylase of bacillus cereus bq10-s1 (spoii) was cloned into escherichia coli jm 109. a sequenced dna fragment of 2,001 bp contains the beta-amylase gene. the n-terminal sequences (avngkg mnpdykaylmaplkki), the c-terminal sequences (shtssw), and the amino acid sequences of the five regions in the beta-amylase molecules were determined. the mature beta-amylase contains 514 amino acid residues with a molecular mass of 57,885 da. the amino acid sequence homology with those ...19938434930
the xync gene from fibrobacter succinogenes s85 codes for a xylanase with two similar catalytic domains.the xync gene of fibrobacter succinogenes s85 codes for a 66.4-kda xylanase which consists of three distinct domains separated by two flexible regions rich in serine residues. domains a and b of xync code for catalytic domains with 56.5% identity and 9.6% similarity with each other, and both domains share homology with xylanases of ruminococcus flavefaciens, neocallimastix patriciarum, clostridium acetobutylicum, bacillus pumilus, bacillus subtilis, and bacillus circulans. more than 88% of the x ...19938244936
overexpression of the bacillus subtilis and circulans xylanases in escherichia coli.an efficient expression system for a low-molecular mass xylanase in escherichia coli has been developed. a gene encoding the mature bacillus circulans (bc) xylanase was designed to imitate the frequency of degenerate codons used in e. coli. appropriate degenerate codons were used to create multiple unique restriction sites for future mutagenesis studies. the synthetic gene was constructed in two stages, both involving ligation of overlapping oligonucleotides. the synthetic bc gene was then conve ...19938518560
cyclomaltodextrin glucanotransferase from bacillus circulans e 192: nitration with tetranitromethane.nitration of tyrosine residues was performed on bacillus circulans e 192 cyclomaltodextrin glucanotransferase (cgtase) using tetranitromethane (tnm). a maximum of 15 out of 28 tyrosine residues is modified with 8 mm tnm, entailing a concomitant loss of enzymic activity and tryptophan fluorescence. spectroscopic studies suggest that these two phenomena are related to an impairment of the enzyme conformation as a consequence of the tyrosine nitration. the presence of 5 mm acarbose during the cgtas ...19938484906
a 4-amino-2-hydroxybutyrate activating enzyme from butirosin-producing bacillus circulans.an enzyme catalyzing a 4-amino-2-hydroxybutyrate dependent atp-pp(i)-exchange reaction has been partially purified from butirosin-producing cells of b. circulans by ammonium sulfate fractionation, gel filtration or sucrose gradient centrifugation and ion exchange chromatography on qae-sepharose. no reaction was found with 4-aminobutyrate and diaminobutyrate. the protein coeluted with groel, which has been identified by alignment of the internal sequence kdgvitveesk. a molecular mass of about 1,5 ...19937506542
domain structure and multiplicity of raw-starch-digesting amylase from bacillus circulans: extensive proteolysis with proteinase k, endopeptidase glu-c and thermolysin.raw-starch-digesting amylase (rsda) is a key extracellular enzyme of mesophilic bacillus circulans f-2 which uses raw starch granules as a carbon source. previous work has demonstrated that there are two domains of the enzyme during digestion with subtilisin, and that rsda activity is selectively inactivated by limited proteolysis with subtilisin, which cleaves the enzyme between these hydrolytic and adsorption domains (kim, c.-h., kwon, s.-t., taniguchi, h. and lee, d.-s. (1992) biochim. biophy ...19937691184
structure of the 87-kda beta-1,3-glucanase gene of bacillus circulans iam1165 and properties of the enzyme accumulated in the periplasm of escherichia coli carrying the gene.the nucleotides of a gene for the extracellular 87-kda beta-1,3-glucanase of bacillus circulans iam1165 and its flanking regions were sequenced. the sequence showed an open reading frame for 877 amino acids, which corresponds to a precursor of the beta-1,3-glucanase. the coding region of 2631 bp is flanked by putative promoter and transcription terminator sequences. the signal peptide was considered to be consisted of 38 amino acids. the amino acid sequence of the mature enzyme composed of 839 a ...19937764221
nucleotide sequence and analysis of a gene (chia) for a chitinase from streptomyces lividans 66.a chitinase gene (chia) from streptomyces lividans was characterized and its nucleotides sequenced. although the deduced amino acid sequence of chitinase a1 did not show any similarity to those of other streptomyces chitinases that has been sequenced, the c-terminal part, containing both a putative catalytic domain and type-iii-like repeating units, showed a similarity (36%) to that of chitinase d from bacillus circulans. a site of initiation of transcription was found approximately 51 bp upstre ...19937764265
expression of an 87-kd-beta-1,3-glucanase of bacillus circulans iam1165 in saccharomyces cerevisiae by low-temperature incubation.a dna segment encoding a signal peptide from yeast invertase was fused in frame to bglh gene encoding 87-kd-beta-1,3-glucanase from bacillus circulans iam1165 and was expressed in the yeast saccharomyces cerevisiae under the control of the gal1 gene promoter. yeast cells containing this fused gene produced active beta-1,3-glucanase in the medium after a long period of incubation at low temperature. the enzyme produced by yeast was heterogeneous in size, and larger than the enzyme produced by esc ...19937764362
direct sequencing of superoxide dismutase genes from two bacterial strains amplified by polymerase chain reaction.the nucleotides of the mn-superoxide dismutase (sod) gene of bacillus circulans and the fe-sod gene of aerobacter aerogenes were sequenced by pcr. these sod genes were specifically amplified by using oligonucleotide primers corresponding to the amino-terminal amino acid sequences and the antisense strand primer corresponding to the common amino acid sequence near the carboxyl-terminus among various mn- and fe-sods thus far sequenced. the pcr products amplified from b. circulans and a. aerogenes ...19937764218
identification of glutamic acid 204 and aspartic acid 200 in chitinase a1 of bacillus circulans wl-12 as essential residues for chitinase activity.prokaryotic chitinases, class iii plant chitinases, yeast chitinases, and endo-beta-n-acetylglucosaminidases share weak amino acid sequence similarities at the certain region of each enzyme. these regions have been assumed to be important for catalytic activities of the enzymes. to verify this assumption, three amino acid residues (ser-160, asp-200, glu-204) in chitinase a1 of bacillus circulans wl-12 were chosen, based on the amino acid sequence alignment of the regions sharing sequence similar ...19938103047
antimicrobial activity of neutralized extracellular culture filtrates of lactic acid bacteria isolated from a cultured indian milk product ('dahi').neutralized extracellular culture filtrate obtained from isolates of lactobacillus acidophilus, lactobacillus delbruecki ssp. bulgaricus, lactobacillus salivarius and lactococcus lactis ssp. lactis from 'dahi' showed weak to moderate inhibition of staphylococcus aureus, bacillus cereus, escherichia coli, bacillus brevis, bacillus circulans, bacillus coagulans, bacillus laterosporus, bacillus subtilis and pseudomonas aeruginosa when tested by the diffusion agar well assay method. the effective mi ...19938110603
microbial leaching of lateritic nickel ore.lateritic nickel ore from the sukinda mines, orissa, india, was leached using thiobacillus ferrooxidans, bacillus circulans, bacillus licheniformis and aspergillus niger at 5% (w/v) solid: liquid ratio for 5-20 days. maximum leaching of ni was achieved with b. circulans (85%) and aspergillus niger (92%) after 20 days. bacillus circulans showed significantly higher rate of leaching than the other organisms giving 80% ni extraction after 15 days. the importance and usefulness of heterotrophic orga ...199324419960
hydrolysis of lactose in skim milk by immobilized beta-galactosidase (bacillus circulans).a novel chemical reactor, consisting of beta-galactosidase from bacillus circulans immobilized onto a ribbed membrane made from polyvinylchloride and silica, was used to hydrolyze the lactose constituent of skim milk. multiresponse nonlinear regression methods were employed to determine the kinetic parameters of rate expressions based on a proposed enzymatic mechanism that includes the formation of oligosaccharides. high-performance liquid chromatography (hplc) methods were employed to monitor t ...199218600962
[selection and physiological study of culture of bacillus circulans-- producer of butirosin].for isolating a highly active variant of the butirosin-producing culture, a strain forming trace amounts of the antibiotic substance was used. exposure to nitrosomethylbiuret and nitrosoguanidine and the use of selective media containing streptomycin and butirosin resulted in a 30-fold increase in the strain productivity. thin layer chromatography of the produced antibiotic substance in the solvent system developed by the authors, mass spectrometry and assay of the antimicrobial spectrum in rega ...19921384451
proteolytic modification of raw-starch-digesting amylase from bacillus circulans f-2 with subtilisin: separation of the substrate-hydrolytic domain and the raw substrate-adsorbable domain.raw starch-digesting amylase (bf-2a, 93,000 da) from bacillus circulans f-2 was converted into two components during digestion with subtilisin. the two components were separated and designated bf-2a' (63 kda) and bf-2b (30 kda), respectively. bf-2a' exhibited the same hydrolysis curve for soluble starch as the original amylase (bf-2a). moreover, the catalytic activities of original and modified enzymes were indistinguishable in km, vmax and in their specific activity for soluble starch hydrolysi ...19921380302
enzymatic synthesis of p-nitrophenyl 4(5)-o-beta-d-galactosyl-alpha-maltopentaoside as a substrate for human alpha-amylases.enzymatic modification at the nonreducing end d-glucosyl residue of p-nitrophenyl alpha-maltopentaoside was developed by using the transglycosylation of beta-d-galactosidase from bacillus circulans. the enzyme regioselectively synthesized p-nitrophenyl 4(5)-o-beta-d-galactosyl-alpha-maltopentaoside (a yield of 12.0% based on the amount of p-nitrophenyl alpha-maltopentaoside added) on a preparative scale from lactose as a donor and p-nitrophenyl alpha-maltopentaoside as an acceptor. it revealed t ...19921377891
primary structure of a chitinase-encoding gene (chi1) from the filamentous fungus aphanocladium album: similarity to bacterial chitinases.chitinase 1 (chi1) is the major extracellular chitinase from the hyperparasitic fungus, aphanocladium album. we determined the complete sequence of the chromosomal and cdna copies of the structural gene (chi1) coding for chi1. the coding region is interrupted by three short introns (55, 53 and 49 bp long). chi1 is 423 aa long and begins with a stretch of 34 aa not found in the mature protein. the chi1 sequence presents overall similarities with bacterial chitinases from serratia marcescens and b ...19921398137
purification, properties, and partial amino acid sequence of chitinase from a marine alteromonas sp. strain o-7.chitinase (ec 3.2.1.14) was isolated from the culture supernatant of a marine bacterium, alteromonas sp. strain o-7. the enzyme (chi-a) was purified by anion-exchange chromatography (deae-toyopearl 650 m) and gel filtration (sephadex g-100). the purified enzyme showed a single band on sodium dodecyl sulfate polyacrylamide gel electrophoresis. the molecular size and pi of chi-a were 70 kda and 3.9, respectively. the optimum ph and temperature of chi-a were 8.0 and 50 degrees c, respectively. chi- ...19921464065
production of cyclomaltodextrin glucanotransferase of bacillus circulans var. alkalophilus atcc21783 in b. subtilis.the cyclomaltodextrin glucanotransferase (cgtase, e.c. 2.4.1.19) gene from an alkalophilic bacillus circulans var. alkalophilus atcc21783 was cloned into escherichia coli and b. subtilis. when cloned from e. coli to b. subtilis, the entire insert containing the cgtase gene was, depending on the plasmid construction, either unstable or the recombinant b. subtilis did not secrete the enzyme in significant amounts. to achieve efficient enzyme production in b. subtilis, the gene was placed under the ...19921368772
construction of an escherichia coli export-affinity vector for expression and purification of foreign proteins by fusion to cyclomaltodextrin glucanotransferase.a novel export-affinity fusion vector employing the gene encoding cyclomaltodextrin glucanotransferase (cgtase; cgt) from bacillus circulans var. alkalophilus (atcc 21783) is described. cgtase binds to various sugar polymers, which makes it simple to purify it to near homogeneity in a single step. the cgtase fusion protein vector was constructed by deleting the translational stop codons from the gene encoding cgtase (cgt) by in vitro mutagenesis. as models, genes encoding escherichia coli alkali ...19921368771
chemical modification of cyclomaltodextrin glucanotransferase from bacillus circulans var. alkalophilus.counting of integral numbers of cysteine residues of the reduced and denaturated form of cyclomaltodextrin glucanotransferase (cgtase) from bacillus circulans var. alkalophilus (atcc 21783) showed two cysteine residues per enzyme molecule. titrations of the enzyme with 5,5'-dithiobis-(2-nitrobenzoic acid) led to the same result. no free sh-group was detected in denatured form of cgtase, indicating that the two cysteine residues are linked by one disulfide bridge. cyclizing activity of the gdmcl- ...19921385982
catalytic center of cyclodextrin glycosyltransferase derived from x-ray structure analysis combined with site-directed mutagenesis.an x-ray structure analysis of a crystal of mutant asp229----ala of cyclodextrin glycosyltransferase from bacillus circulans (ec 2.4.1.19) that had been shortly exposed to beta-cyclodextrin showed density corresponding to a maltose bound at the catalytic center. the crystal structure was refined to an r-factor of 18.7% at 2.5-a resolution. the catalytic center is defined by homology with the structurally known alpha-amylases and by the observation that mutants asp229----ala and asp328----ala are ...19921390660
purification and some properties of l-fucose dehydrogenase from agrobacterium radiobacter and its application to the assay of bound-fucose in glycoconjugates.l-fucose dehydrogenase was found in the cell extract of agrobacterium radiobacter and purified to homogeneity about 480-fold with 16% recovery. the molecular weight of the enzyme was approx. 64,000. the enzyme was active in the neutral ph range, unlike other l-fucose or d-arabinose dehydrogenases which are active only in the alkaline ph range. using this enzyme and alpha-l-fucosidase f-i of bacillus circulans (tsuji, y., yamamoto, k., tochikura, t., seno, t., ohkubo, y. and yamaguchi, h. (1990) ...19921525177
cyclomaltodextrin glucanotransferase from bacillus circulans e 192. i. purification and characterization of the enzyme.the cyclomaltrodextrin glucanotransferase (cgtase) [1,4-alpha-d-glucan:4-alpha-d-(1,4-alpha-d-glucano)-transferase (cyclizing), ec 2.4.1.19] from bacillus circulans e 192 has been purified to homogeneity by cetavlon treatment, ammonium sulfate precipitation, deae trisacryl m chromatography, q fast flow chromatography, and affinity on beta-cyclodextrin-sepharose 4b. two isoenzymes were separated by fplc on a mono q column. their isoelectric points were estimated as 6.7 and 6.9 and they represente ...19921532314
isolation and partial characterization of an 87-kilodalton beta-1,3-glucanase from bacillus circulans iam1165.bacillus circulans iam1165 produces at least two extracellular beta-1,3-glucanases that lyse fungal cell walls. one of these extracellular enzymes was purified to homogeneity. the molecular mass was 87 kda, and the pi was 4.3. the optimum temperature of the enzyme reaction was 70 degrees c when laminarin (a soluble beta-1,3-glucan) was used as the substrate. the ph range of the enzyme was broad (ph 4.5 to 9.0), and the optimum ph was 6.5. the enzyme is an endo beta-1,3-glucanase and has a random ...19921610176
galactosylation at side chains of branched cyclodextrins by various beta-galactosidases.the galactosyl transfer reaction to branched cyclodextrins (cds) was investigated using lactose as a donor substrate and branched cds as acceptors by various beta-galactosidases. bacillus circulans beta-galactosidase synthesized galactosyl transfer products to branched cds, of which the galactose residues were linked at side chains of branched cds, not directly at cd rings. aspergillus oryzae and penicillium multicolor beta-galactosidases also produced derivatives galactosylated at side chains o ...19921368300
[cloning and expression of alpha-hydroxy-gamma-aminobutyl acylase gene of bacillus circulans nrrl-b3312].with shot-gun cloning strategy, we used pub110 plasmid as a vactor to clone dna fragment of bacillus circulans nrrl-b3312, which is butirosin producer, into bacllus subtilis 168. among the transformants, the results of tlc, bioautography and fab mass, spectrum analysis for the bioconversion product of no. 733 transformant showed that this transformant could transform kanamycin into amikacin. according to these results, the haba acylase gene locates on the insert fragment of pubc733 plasmid harbo ...19921305824
three n-terminal domains of beta-1,3-glucanase a1 are involved in binding to insoluble beta-1,3-glucan.limited proteolysis of beta-1,3-glucanase a1 by three different proteases, trypsin, chymotrypsin, and papain, gave three major active fragments. the sizes of the three major fragments generated by each protease treatment were identical to those of beta-1,3-glucanase a2, a3, and a4 detected in both the culture supernatant of bacillus circulans wl-12 and the periplasmic space of escherichia coli carrying a cloned glca gene. these results indicate a four-domain structure for the enzyme. at the n te ...19921729208
structure of the gene encoding chitinase d of bacillus circulans wl-12 and possible homology of the enzyme to other prokaryotic chitinases and class iii plant chitinases.the gene (chid) encoding the precursor of chitinase d was found to be located immediately upstream of the chia gene, encoding chitinase a1, which is a key enzyme in the chitinase system of bacillus circulans wl-12. sequencing analysis revealed that the deduced polypeptide encoded by the chid gene was 488 amino acids long and the distance between the coding regions of the chia and chid genes was 103 bp. remarkable similarity was observed between the n-terminal one-third of chitinase d and the c-t ...19921729234
characterisation of bcibii, an isoschizomer of bstni from a strain of bacillus circulans b. 19921614883
production of a novel extracellular polysaccharide by a bacillus strain isolated from soil.a bacterium that was isolated from soil and identified as bacillus circulans was found to produce a highly viscous extracellular polysaccharide when it was grown aerobically in a medium containing glucose as a sole source of carbon. the product was characterized by tlc and gc analyses as a novel heteropolysaccharide consisted of rhamnose, mannose, galactose, and mannuronic acid as sugar components. a maximal yield of polysaccharide reached about 2 g/liter by jar-fermentor culture at 30°c for 48 ...199227280807
purification and some properties of chitinase b1 from bacillus circulans wl-12. 199227280674
properties of a highly viscous polysaccharide produced by a bacillus strain isolated from soil.chemical and theological properties of a highly viscous and acidic polysaccharide produced by a soil bacterium identified as bacillus circulans are described. the molecular weight of the native polysaccharide was about 116 × 10(4) by gel filtration with hplc. the molar ratio of d-galactose, n-mannose, and l-rhamnose contained in the polysaccharide as neutral sugar components was 1.3:1:2. the viscosity of 1% solution of the polysaccharide was about 5000 cp, which was higher than that of guar gum, ...199227280661
exopolysaccharide structure from bacillus circulans. 19912001692
structure of cyclodextrin glycosyltransferase refined at 2.0 a resolution.the previously reported structural model of cyclodextrin glycosyltransferase (ec 2.4.1.19) from bacillus circulans has been improved. for this purpose the known sequence was built into an electron density map established by multiple isomorphous replacement and subsequent solvent-flattening at 2.5 a resolution. the resulting model was refined at 2.0 a resolution using a simulated annealing refinement method. based on 70,171 independent reflections in the range 7.0 to 2.0 a resolution, a final r-f ...19911826034
endocarditis caused by bacillus circulans. 19911816117
on the role of histidine residues in cyclodextrin glycosyltransferase: chemical modification with diethyl pyrocarbonate.ethoxyformylation with diethyl pyrocarbonate of approximately 1.5 his residues per molecule of enzyme reduced the cyclising activity of both the alpha-cyclodextrin glycosyltransferase from klebsiella pneumoniae strain m 5 al and the beta-cyclodextrin glycosyltransferase from bacillus circulans strain 8 by greater than 90%. pre-incubation with substrate protected the enzymes from ethoxyformylation. digestion of starch by the modified enzymes resulted in a delayed formation of cyclodextrins (cyclo ...19911828005
microbial metabolism of quinoline and related compounds. ix. degradation of 6-hydroxyquinoline and quinoline by pseudomonas diminuta 31/1 fa1 and bacillus circulans 31/2 a1.two strains, using 6-hydroxyquinoline as sole source of energy, carbon and nitrogen, have been isolated. these bacteria, designated 31/1 fa1 and 31/2 a1, are also able to degrade quinoline. according to their physiological properties strain 31/1 fa1 has been identified as pseudomonas diminuta and strain 31/2 a1 as bacillus circulans. 6-hydroxy-2-oxo-1,2-dihydroquinoline was found as intermediate in the degradation of 6-hydroxyquinoline and quinoline. 2-oxo-1,2-dihydroquinoline was the first meta ...19911910576
complete amino acid sequence of endo-beta-n-acetylglucosaminidase from flavobacterium sp.the complete amino acid sequence of endo-beta-n-acetylglucosaminidase from flavobacterium sp. has been determined by analysis of peptides after cleavage with lysyl endopeptidase, pepsin and chymotrypsin. the protein consists of a single polypeptide chain consisting of 267 amino acid residues and a molecular mass of 27972 da. the sequence of flavobacterium endo-beta-n-acetylglucosaminidase is very close to that of the streptomyces enzyme (endo-h), having 60% similarity and very similar hydropathy ...19911935974
enzymic synthesis of 4(5)-o-beta-galactosyl-maltopentaose by bacillus circulans beta-galactosidase. 19911368744
multiple domains in endoglucanase b (cenb) from cellulomonas fimi: functions and relatedness to domains in other polypeptides.endoglucanase b (cenb) from the bacterium cellulomonas fimi is divided into five discrete domains by linker sequences rich in proline and hydroxyamino acids (a. meinke, c. braun, n. r. gilkes, d. g. kilburn, r. c. miller, jr., and r. a. j. warren, j. bacteriol. 173:308-314, 1991). the catalytic domain of 608 amino acids is at the n terminus. the sequence of the first 477 amino acids in the catalytic domain is related to the sequences of cellulases in family e, which includes procaryotic and euca ...19911938913
the interaction of xylanases with commercial pulps.when purified xylanases from trichoderma harzianum e58 or from a clone of bacillus circulans were incubated with various low-yield wood pulps, little of the original enzyme activity could be detected in the filtrate at the end of the reaction. partial bleaching of the pulps prior to enzymatic treatment generally resulted in an increased recovery of the xylanase activity. it appears that both nonspecific adsorption and soluble inhibitors may be responsible for the loss of much of the xylanase act ...199118597365
synthesis and secretion of a bacillus circulans wl-12 1,3-1,4-beta-d-glucanase in escherichia coli.the synthesis and secretion of a 1,3-1,4-beta-d-glucanase were studied in different strains of escherichia coli transformed with plasmids carrying the bacillus circulans wl-12 1,3-1,4-beta-d-glucanase structural gene. this gene (named bgc) is contained within a 1.9-kilobase bamhi-hindiii fragment and directs the synthesis in e. coli of an enzyme that specifically degrades lichenan. only one active form of the enzyme was found when the gene was expressed in different e. coli strains. the electrop ...19902180919
cloning of two genes from bacillus circulans wl-12 which encode 1,3-beta-glucanase activity.two genes encoding distinct 1,3-beta-glucanases have been cloned from bacillus circulans and expressed in escherichia coli. a cosmid library of b. circulans wl-12 dna was constructed in the broad-host-range cosmid plafr1 and screened in e. coli for clones which exhibited 1,3-beta-glucanase activity. two 1,3-beta-glucanase-positive clones were identified which contained genes encoding two independent 1,3-beta-glucanases as shown by biochemical, physical and molecular analyses. the cosmids, design ...19902127800
highly homologous cyclodextrin glycosyltransferases from bacillus circulans strain 8 and a strain of bacillus licheniformis. 19901368625
molecular cloning, nucleotide sequence and expression in escherichia coli of the beta-cyclodextrin glycosyltransferase gene from bacillus circulans strain no. 8.the beta-cyclodextrin glycosyltransferase (beta-cgtase) gene was isolated from a lambda-library prepared from bacillus circulans strain no. 8. it was subcloned into plasmid ptz and expressed by its endogenous regulatory sequences in escherichia coli jm 103. the structural gene was sequenced and showed an open reading frame for a polypeptide of 718 amino acid residues. the recombinant beta-cgtase had the same enzymatic properties as the extracellular cgtase (684 amino acid residues, corresponding ...19901368573
purification and characterization of alpha-l-fucosidase from bacillus circulans grown on porcine gastric mucin.bacillus circulans isolated from soil was found to produce two types of alpha-l-fucosidase differing in substrate specificity. one was able to liberate l-fucose from porcine gastric mucin (pgm), but not from artificial substrates, including p-nitrophenyl and methyl alpha-l-fucosides, while the other acted not on pgm but on p-nitrophenyl alpha-l-fucoside. the production of the former enzyme was enhanced about 150 times as much by pgm added to the medium as by glucose. the alpha-l-fucosidase actin ...19901694531
maltodextrin-dependent crystallization of cyclomaltodextrin glucanotransferase from bacillus circulans.crystals of cyclomaltodextrin glucanotransferase from bacillus circulans (ec 2.4.1.19) suitable for high-resolution x-ray analysis were obtained by vapor diffusion against 60% (v/v) 2-methyl 2,4-pentanediol buffered with 100 mm-sodium hepes, ph 7.55. the crystals have p2(1)2(1)2(1) space group symmetry, with a = 120.4 a, b = 110.9 a and c = 66.4 a, and contain one molecule of 68,000 in the asymmetric unit. growth of single enzyme crystals was found to require the presence of either alpha-cyclode ...19902143786
cloning and expression of raw-starch-digesting alpha-amylase gene from bacillus circulans f-2 in escherichia coli.the raw potato-starch-digesting alpha-amylase gene of bacillus circulans f-2 was cloned for the first time in escherichia coli c600, using plasmid pyej001. the recombinant plasmid, named pyka3, has a 5.4 kb insert from a chromosome of the donor bacterium. subcloning of this amylase gene gave plasmid pha300 which carried 3.15 kb of the inserted dna. the transformed bacterium, e. coli c600 (pyka3), produced the amylase in the periplasmic space, whereas it is secreted outside the cell in the donor ...19902182125
gene cloning of chitinase a1 from bacillus circulans wl-12 revealed its evolutionary relationship to serratia chitinase and to the type iii homology units of fibronectin.a chitinase gene of bacillus circulans wl-12 was cloned into escherichia coli by transforming hb101 cells with a recombinant plasmid composed of chromosomal dna fragments prepared from b. circulans wl-12 and the plasmid vector pkk223-3. dna sequencing analysis revealed that the region necessary for the normal expression of chitinase activity contained one open reading frame of 2097 base pairs which codes for the precursor of chitinase a1. the precursor of chitinase a1 contained a long signal seq ...19902203782
the chromosomal integration site of the streptomyces element psam2 overlaps a putative trna gene conserved among actinomycetes.the psam2 element of streptomyces ambofaciens integrates site-specifically in the genome of different streptomyces species by recombination between a 58 bp sequence common to the plasmid (attp) and the chromosome (attb). southern hybridization analysis showed that sequences similar to the psam2 attb site were found in other actinomycetes (mycobacterium, nocardia, micromonospora) as well as unrelated bacteria (bacillus circulans, escherichia coli, clostridium botulinum, bordetella pertussis, and ...19901703270
molecular cloning, expression, and characterization of endo-beta-1,4-glucanase genes from bacillus polymyxa and bacillus circulans.endo-beta-1,4-glucanase genes from bacillus circulans and from b. polymyxa were cloned by direct expression by using bacteriophage m13mp9 as the vector. the enzymatic activity of the gene products was detected by using either the congo red assay or hydroxyethyl cellulose dyed with ostazin brilliant red h-3b. the b. circulans and b. subtilis pap115 endo-beta-1,4-glucanase genes were shown to be homologous by the use of restriction endonuclease site mapping, dna-dna hybridization, s1 nuclease dige ...19902307659
structure of the gene encoding beta-1,3-glucanase a1 of bacillus circulans wl-12.the nucleotide sequence of the glca gene encoding the precursor of extracellular beta-1,3-glucanase (beta gl) a1, a polysaccharidase produced by bacillus circulans wl-12, was determined. the putative glca gene was 2046 bp long, encoding a polypeptide of 682 amino acids (aa). the n-terminal aa sequence of beta gl produced in escherichia coli harboring the glca plasmid was identical to that of beta gl a1 prepared from the culture fluid of b. circulans wl-12. in both proteins, cleavage of the signa ...19902311931
chitinase system of bacillus circulans wl-12 and importance of chitinase a1 in chitin degradation.bacillus circulans wl-12, isolated as a yeast cell wall-lytic bacterium, secretes a variety of polysaccharide-degrading enzymes into culture medium. when chitinases of the bacterium were induced with chitin, six distinct chitinase molecules were detected in the culture supernatant. these chitinases (a1, a2, b1, b2, c, and d) showed the following distinct sizes and isoelectric points: mr 74,000, pi 4.7 (a1); mr 69,000, pi 4.5 (a2); mr 38,000, pi 6.6 (b1); mr 38,000, pi 5.9 (b2); mr 39,000, pi 8.5 ...19902361948
analysis of cyclomaltodextrin glucanotransferase isoenzymes by isoelectric focusing in immobilized ph gradients.the catalytically active subforms of cyclomaltodextrin glucanotransferase (cgtase; ec 2.4.1.19) from bacillus circulans var. alkalophilus and from a strain in which the cgtase expressing gene had been cloned were studied by using isoelectric focusing (ief) in immobilized and in conventional ph gradients. even with high protein loads the best resolution was achieved in immobilized ph gradients (ipg). native cgtase, focused on ipg 4.5-5.4, was resolved into more than 6 subforms, a major one with p ...19902140579
nucleotide sequence of a 1,3-1,4-beta-glucanase-encoding gene in bacillus circulans wl-12. 19902377467
purification and characterization of an isoschizomer of sphi from bacillus circulans. 19902146591
engineering a heavy atom derivative for the x-ray structure analysis of cyclodextrin glycosyltransferase.based on a preliminary structural model of cyclodextrin glycosyltransferase from bacillus circulans (ec 2.4.1.19), ser428 and ser475 of the enzyme were mutated to cysteines in order to produce suitable heavy atom derivatives. mutant ser475----cys could not be expressed as protein. mutant ser428----cys was expressed in escherichia coli and purified. it crystallized isomorphously and gave rise to a mercury derivative that improved the electron density map. the structural results show that the new ...19902149760
studies of the mechanism of the cyclisation reaction catalysed by the wildtype and a truncated alpha-cyclodextrin glycosyltransferase from klebsiella pneumoniae strain m 5 al, and the beta-cyclodextrin glycosyltransferase from bacillus circulans strain 8.the actions of the wildtype and a truncated alpha-cyclodextrin glycosyltransferase from klebsiella pneumoniae strain m 5 al on malto-oligosaccharides showed no significant differences, and there was marked dependence of the kinetic parameters on the chain lengths of the substrate. the action of the beta-cyclodextrin glycosyltransferase from bacillus circulans was less dependent on the chain length of the substrate, but vmax of the initial cyclisation with the longer malto-oligosaccharides was on ...19902150008
cloning of aminoglycoside phosphotransferase (aph) gene from antibiotic-producing strain of bacillus circulans into a high-expression vector, pkk223-3. purification, properties and location of the enzyme.the aminoglycoside phosphotransferase gene from a butirosin-producing strain of bacillus circulans was cloned in a high-expression vector (pkk223-3) to give the recombinant plasmid pms5. escherichia coli harbouring the plasmid, e. coli jm103[pms5], was characterized, and several features of the expression of the phosphotransferase were studied. the phosphotransferase activity was best expressed in a medium lacking glucose, and the highest levels of the enzyme were found between 12 and 24 h of gr ...19902163618
purification and some properties of a novel alpha-l-fucosidase capable of acting on alpha-(1----6)-l-fucosidic linkages from bacillus circulans m28.two types of alpha-l-fucosidase (f-i and f-ii), that differ in substrate specificity, were produced in the culture fluid by bacillus circulans isolated from soil when the bacterium was cultivated on medium containing porcine gastric mucin. f-i was able to cleave the alpha-(1----2), alpha-(1----3), and alpha-(1----4)-l-fucosidic linkages in various oligosaccharides and glycoproteins, but not p-nitrophenyl alpha-l-fucoside, as previously reported [y. tsuji et al. (1990) j. biochem. 107, 324-330]. ...19902172224
formation of a cycloinulo-oligosaccharide from inulin by an extracellular enzyme of bacillus circulans okumz 31b.a strain of bacillus circulans okumz 31b, isolated from soil, has been shown to produce an extracellular enzyme that converts inulin into cycloinulo-oligosaccharides. the main product was identified as cycloinulo-hexaose. the enzyme is arbitrarily designated as cycloinulo-oligosaccharide fructanotransferase.19892611778
hyperexpression of a bacillus circulans xylanase gene in escherichia coli and characterization of the gene product.a 4.0-kilobase (kb) fragment of bacillus circulans genomic dna inserted into puc19 and encoding endoxylanase activity was subjected to a series of subclonings. a 1.0-kb hindiii-hincii subfragment was found to code for xylanase activity. maximum expression levels were observed with a subclone that contained an additional 0.3-kb sequence upstream from the coding region. enhancer sequences in the upstream region are thought to be responsible for these high expression levels. southern hybridization ...19892667461
structure and glycosylation of lipoteichoic acids in bacillus strains.the occurrence, structure, and glycosylation of lipoteichoic acids were studied in 15 bacillus strains, including bacillus cereus (4 strains), bacillus subtilis (5 strains), bacillus licheniformis (1 strain), bacillus polymyxa (2 strains), and bacillus circulans (3 strains). whereas in the cells of b. polymyxa and b. circulans neither lipoteichoic acid nor related amphipathic polymer could be detected, the cells of other bacillus strains were shown to contain lipoteichoic acids built up of poly( ...19892914853
three-dimensional structure of cyclodextrin glycosyltransferase from bacillus circulans at 3.4 a resolution.cyclodextrin glycosyltransferase (ec 2.4.1.19) from bacillus circulans has been purified, crystallized and analyzed by x-ray diffraction. the enzyme is monomeric. sds/polyacrylamide gel electrophoresis gave an mr of 73,600(+/- 1000), corresponding to 670(+/- 10) amino acid residues. the structure of the crystalline enzyme has been elucidated at a resolution of 3.4 a, using multiple isomorphous replacement and solvent flattening for phase determination. the resulting electron density map allowed ...19892531228
identification of two distinct bacillus circulans xylanases by molecular cloning of the genes and expression in escherichia coli.two genes coding for xylanase synthesis in bacillus circulans were cloned and expressed in escherichia coli. after digestion of genomic dna from bacillus circulans with ecori and psti, the fragments were ligated into the corresponding sites of puc19 and transformed into escherichia coli. restriction enzyme mapping of the two inserts coding for xylanase activity indicated distinctly different nucleotide sequences. cross-hybridization assays confirmed the absence of sequence homology between the t ...19892648989
two-stage reimplantation in infected total knee arthroplasty.twenty-one infected total knee arthroplasties (tka) in 21 patients were treated from september 1980 through october 1987. of these, 15 were followed for more than one year. treatment of these patients consisted of thorough debridement of all infected tissue and components; a cement spacer was used in ten patients. the cement was impregnated with antibiotics. this procedure was followed for an average of 4.2 weeks with intravenous antibiotics and tka utilizing antibiotic-impregnated cement. five ...19883180576
alginase treatment of mucoid pseudomonas aeruginosa enhances phagocytosis by human monocyte-derived macrophages.mucoid pseudomonas aeruginosa colonizes and infects the respiratory tract of most older patients with cystic fibrosis. these bacteria resist both opsonin-dependent and -independent phagocytosis by human polymorphonuclear leukocytes and monocyte-derived macrophages. resistance to phagocytosis is thought to be mediated in part by the mucoid exopolysaccharide associated with the bacterial surface. the purpose of this study was to determine whether degradation of the mucoid exopolysaccharide by algi ...19883139564
nucleotide sequence of a bacillus circulans xylanase gene. 19883405767
bacillus infections in patients with cancer.eighteen febrile patients experienced 24 episodes of bacillus bacteremias from january 1978 to june 1986. bacillus species isolated included bacillus cereus (eight cases), bacillus circulans (three), bacillus subtilis (two), bacillus pumilus (two), bacillus licheniformis (one), bacillus sphaericus (one), bacillus coagulans (one), and six that could not be speciated. fifteen patients had lymphoma or leukemia and three had breast cancer. nine patients were neutropenic (polymorphonuclear neutrophil ...19883401098
molecular cloning of an amylase gene of bacillus circulans.an amylase gene of bacillus circulans was cloned in b. subtilis and its nucleotide sequence was determined. the putative proamylase consists of 528 amino acids, which correspond to a molecular weight of 58,776. homologous regions with other amylases of bacillus species were found. a sigma 55-type promoter is located at about 250 bp upstream from the starting codon. this promoter was also functional in escherichia coli, and able to express beta-galactosidase activity.19873109866
alginate lyase releases cell-bound lipase from mucoid strains of pseudomonas aeruginosa.alginate lyase (ec 4.2.2.3) was partially purified from the culture medium of bacillus circulans jbh2 by ammonium sulphate precipitation, gel filtration and ion-exchange chromatography. the purified enzyme was unstable in the absence of protecting substances such as gelatin. at a ph optimum of 5.8, the enzyme depolymerized algal as well as bacterial alginates, the latter in the deacetylated form more effectively than in the acetylated form. incubation of various mucoid strains of pseudomonas aer ...19873125706
microorganisms capable of metabolizing the herbicide metolachlor.we screened several strains of microorganisms and microbial populations for their ability to mineralize or transform the herbicide metolachlor [2-chloro-n-(2-ethyl-6-methylphenyl)-n-(2-methoxy-1-methylethyl)-acetami de] because such cultures would potentially be useful in the cleanup of contaminated sites. although we used various inocula and enrichment culture techniques, we were not able to isolate microorganisms that could mineralize metolachlor. however, strains of bacillus circulans, bacill ...19873105457
molecular cloning and characterization of the beta-amylase gene from bacillus circulans.a gene encoding the beta-amylase of bacillus circulans was isolated from a lambda library and sequenced. the structural gene consists of a 1725 bp open reading frame encoding a polypeptide with a predicted molecular wt of 62830 daltons. two active forms of the enzyme were found when the gene was expressed in e. coli. the larger 60 kd form was approximately 3 kd larger than the mature beta-amylase secreted from b. circulans, suggesting that processing of this protein is different between the two ...19872455212
bagougeramines a and b, new nucleoside antibiotics produced by a strain of bacillus circulans. ii. physico-chemical properties and structure determination.bagougeramines a and b obtained as sulfates were soluble in water and positive to sakaguchi, chlorine-tolidine and ninhydrin color reactions. their structures were determined by acid hydrolysis and spectroscopic analysis. structurally they were closely related to gougerotin and they contained the guanidino-d-alanine instead of the serine residue in gougerotin. bagougeramine b had the spermidine instead of the 6'-nh2 in structure of bagougeramine a.19863759654
sequence and interspecies transfer of an aminoglycoside phosphotransferase gene (aph) of bacillus circulans. self-defence mechanism in antibiotic-producing organisms.the aph gene of a butirosin-producing bacillus circulans was cloned and shown to be expressed in escherichia coli and streptomyces lividans. the gene was sequenced and a possible developmentally regulated promoter identified. when the deduced protein sequence was compared with those from transposon tn5, transposon tn903, streptomyces fradiae, staphylococcus aureus and streptococcus faecalis, significant homology was found, indicating that the genes may have a common origin.19863006668
bagougeramines a and b, new nucleoside antibiotics produced by a strain of bacillus circulans. i. taxonomy of the producing organism and isolation and biological properties of the antibiotics.a bacterial isolate from soil, designated as tb-2125 had a unique pattern of multiple resistance to aminoglycoside antibiotics (ag) and produced new nucleoside antibiotics. taxonomic properties of this strain fell into those of bacillus circulans, providing unique characteristics such as strict susceptibility to acidic ph, motility of colony as well as multiple ag-resistance. two new antibiotics which were named bagougeramines a and b had a broad antimicrobial activity and a specific activity ag ...19863759653
evolution and transfer of aminoglycoside resistance genes under natural conditions.3'-aminoglycoside phosphotransferases [aph(3')] were chosen as a model to study the evolution and the transfer of aminoglycoside resistance genes under natural conditions. comparison of the amino acid sequences of aph(3') enzymes from transposons tn903 (type i) and tn5 (type ii) detected in gram-negative bacteria, from the gram-positive staphylococcus and streptococcus (type iii), from the butirosin-producing bacillus circulans (type iv) and from a neomycin-producing streptomyces fradiae (type v ...19863027020
isolation of pathogenic bacillus circulans from callus cultures and healthy offshoots of date palm (phoenix dactylifera l.).pure cultures of a gram-negative, rod-shaped bacterium, which produced endospores after 3 to 5 days on solid medium, were isolated exclusively from tissue cultures of the date palm phoenix dactylifera l. electron microscopic examination of thin sections of the bacteria revealed the bilayer membrane typical of gramnegative bacteria and confirmed the nature of the spores as true endospores. biochemical and physiological tests indicated that the bacteria were bacillus circulans. b. circulans was co ...198616347217
distribution of alginate lyase activity among strains of bacillus circulans.strains from four different dna relatedness groups of bacillus circulans showed apparent alginate lyase activity; the activity of three strains examined had mannuronidase specificity. a representative strain of group 4 also produced apparent inducible unsulfated chrondroitin lyase activity.198516346766
relationship between butirosin biosynthesis and sporulation in bacillus circulans.the relationship between butirosin biosynthesis and certain biochemical characteristics related to sporulation in a strain of bacillus circulans nrrl b-3313 was examined. the cellular content of dipicolinic acid increased while the amount of poly-beta-hydroxybutyrate decreased with changes in antibiotic productivity. oligosporogenous mutants failed to synthesize the antibiotic and to degrade poly-beta-hydroxybutyrate. these observations suggest that spore formation may be related to antibiotic p ...19852409916
isolation of bacillus circulans from a wound infection. 19854019805
[infection of a cerebrospinal fluid shunt system by bacillus circulans and bacillus larvae].a two episodes case of csf ventriculo-atrial shunt infection due to b. circulans and b. larvae is presented. b. circulans was first isolated from 4 blood cultures and csf (shunt valve tap). the patient showed a brain damage syndrome reversible with antibiotic treatment. lethal toxin production was demonstrated for the b. circulans strain in a mouse model. this strain was found to be a variant of gordon's description as it produced urease and was tolerant to 7% nacl. the patient recovered after c ...19853870747
generic composition and physiological and cultural properties of heterotrophic bacteria isolated from soil, rhizosphere and mycorrhizosphere of pine (pinus silvestris l.).among the bacteria studied arthrobacter globiformis was predominating in the root zone, while in the non-rhizosphere soil most numerous were bacillus circulans and a. globiformis. ammonifiers were more numerous among the root zone bacteria than among the root free soil organisms. the reverse was noted with bacteria capable of hydrolysing starch, cellulose, pectin and chitin.19846209931
alginase enzyme production by bacillus circulans.stream and soil samples were screened for microorganisms that would use alginate from mucoid pseudomonas aeruginosa as the sole carbon and energy source. a pure culture containing large aerobic rods was isolated. the cells were about 0.8 by 2.5 microns in size, had lateral or peritrichous flagella, had a negative gram stain reaction, and produced spores on sporulation medium. purified dna was approximately 46 mol% g+c as measured by thermal denaturation. from these and other biochemical tests, t ...19846426387
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