Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| crystallization and x-ray analysis of 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics. | a recombinant 2-deoxy-scyllo-inosose synthase from bacillus circulans has been crystallized at 277 k using peg 4000 as precipitant. the diffraction pattern of the crystal extends to 2.30 a resolution at 100 k using synchrotron radiation at the photon factory. the crystals are monoclinic and belong to space group p2(1), with unit-cell parameters a = 80.5, b = 70.4, c = 83.0 a, beta = 117.8 degrees. the presence of two molecules per asymmetric unit gives a crystal volume per protein weight (vm) of ... | 2005 | 16511136 |
| bacillus circulans mh-k1 chitosanase: amino acid residues responsible for substrate binding. | to identify the amino acids responsible for the substrate binding of chitosanase from bacillus circulans mh-k1 (mh-k1 chitosanase), tyr148 and lys218 of the chitosanase were mutated to serine and proline, respectively, and the mutated chitosanases were characterized. the enzymatic activities of y148s and k218p were found to be 12.5% and 0.16% of the wild type, respectively. when the (glcn)3 binding ability to the chitosanase was evaluated by fluorescence spectroscopy and thermal unfolding experi ... | 2005 | 16272568 |
| biological control of late leaf spot of peanut (arachis hypogaea) with chitinolytic bacteria. | abstract late leaf spot (lls), caused by phaeoisariopsis personata, is a foliar disease of groundnut or peanut (arachis hypogaea) with high economic and global importance. antifungal and chitinolytic bacillus circulans grs 243 and serratia marcescens gps 5, selected among a collection of 393 peanut-associated bacteria, were applied as a prophylactic foliar spray and tested for control of lls. chitin-supplemented application of b. circulans grs 243 and s. marcescens gps 5 resulted in improved bio ... | 2005 | 18943468 |
| production of lewis x tetrasaccharides by metabolically engineered escherichia coli. | two tetrasaccharides carrying the trisaccharidic lewis x motif on a glcnac or a gal residue were produced on the gram-scale by high-cell-density cultures of metabolically engineered escherichia coli strains that overexpressed the helicobacter pylori futa gene for alpha-3 fucosyltransferase and the neisseria meningitidis lgtb gene for beta-4 galactosyltransferase. the first compound galbeta-4(fucalpha-3)glcnacbeta-4glcnac was produced by glycosylation of chitinbiose, which was endogenously genera ... | 2006 | 16381046 |
| enrichment and isolation of a mixed bacterial culture for complete mineralization of endosulfan. | in the present study, we isolated three novel bacterial species, namely, staphylococcus sp., bacillus circulans-i, and bacillus circulans-ii, from contaminated soil collected from the premises of a pesticide manufacturing industry. batch experiments were conducted using both mixed and pure cultures to assess their potential for the degradation of aqueous endosulfan in aerobic and facultative anaerobic condition. the influence of supplementary carbon (dextrose) source on endosulfan degradation wa ... | 2006 | 16393897 |
| identification of the substrate interaction region of the chitin-binding domain of streptomyces griseus chitinase c. | chitinase c from streptomyces griseus hut6037 was discovered as the first bacterial chitinase in family 19 other than chitinases found in higher plants. chitinase c comprises two domains: a chitin-binding domain (chbd(chic)) for attachment to chitin and a chitin-catalytic domain for digesting chitin. the structure of chbd(chic) was determined by means of 13c-, 15n-, and 1h-resonance nuclear magnetic resonance (nmr) spectroscopy. the conformation of its backbone comprised two beta-sheets composed ... | 2006 | 16567413 |
| enzymatic production of highly soluble myricitrin glycosides using beta-galactosidase. | myricitrin, a botanical flavonol glycoside, could be a useful ingredient of functional foods, cosmetics, and medicines because of its high anti-oxidative activity. however, due to its insolubility in water, it has a limited range of use. to improve this solubility, we glycosylated myricitrin by an enzymatic transglycosylate reaction. myricitrin was galactosylated by beta-galactosidase from bacillus circulans using lactose as a sugar donor. the reaction product was 480 times more soluble than myr ... | 2006 | 16636462 |
| bioremediation of endosulfan contaminated soil and water -- optimization of operating conditions in laboratory scale reactors. | a mixed bacterial culture consisted of staphylococcus sp., bacillus circulans-i and -ii has been enriched from contaminated soil collected from the vicinity of an endosulfan processing industry. the degradation of endosulfan by mixed bacterial culture was studied in aerobic and facultative anaerobic conditions via batch experiments with an initial endosulfan concentration of 50mg/l. after 3 weeks of incubation, mixed bacterial culture was able to degrade 71.58+/-0.2% and 75.88+/-0.2% of endosulf ... | 2006 | 16730891 |
| cloning and expression of an alpha-1,3-glucanase gene from bacillus circulans ka-304: the enzyme participates in protoplast formation of schizophyllum commune. | a culture filtrate of bacillus circulans ka-304 grown on a cell-wall preparation of schizophyllum commune has an activity to form protoplasts from s. commune mycelia, and a combination of alpha-1,3-glucanase and chitinase i, which were isolated from the filtrate, brings about the protoplast-forming activity. the gene of alpha-1,3-glucanase was cloned from b. circulans ka-304. it consists of 3,879 nucleotides, which encodes 1,293 amino acids including a putative signal peptide (31 amino acid resi ... | 2006 | 16861810 |
| engineering of cyclodextrin glucanotransferase on the cell surface of saccharomyces cerevisiae for improved cyclodextrin production. | the cyclodextrin glucanotransferase (cgtase) gene (cgt) from bacillus circulans 251 was cloned into plasmid pyd1, which allowed regulated expression, secretion, and detection. the expression of cgtase with a-agglutinin at the n-terminal end on the extracellular surface of saccharomyces cerevisiae was confirmed by immunofluorescence microscopy. this surface-anchored cgtase gave the yeast the ability to directly utilize starch as a sole carbon source and the ability to produce the anticipated prod ... | 2006 | 16517633 |
| crystal structures of the plp- and pmp-bound forms of btrr, a dual functional aminotransferase involved in butirosin biosynthesis. | the aminotransferase (btrr), which is involved in the biosynthesis of butirosin, a 2-deoxystreptamine (2-dos)-containing aminoglycoside antibiotic produced by bacillus circulans, catalyses the pyridoxal phosphate (plp)-dependent transamination reaction both of 2-deoxy-scyllo-inosose to 2-deoxy-scyllo-inosamine and of amino-dideoxy-scyllo-inosose to 2-dos. the high-resolution crystal structures of the plp- and pmp-bound forms of btrr aminotransferase from b. circulans were solved at resolutions o ... | 2006 | 16894611 |
| effect of ph on the structure and stability of bacillus circulans ssp. alkalophilus phosphoserine aminotransferase: thermodynamic and crystallographic studies. | ph is one of the key parameters that affect the stability and function of proteins. we have studied the effect of ph on the pyridoxal-5'-phosphate-dependent enzyme phosphoserine aminotransferase produced by the facultative alkaliphile bacillus circulans ssp. alkalophilus using thermodynamic and crystallographic analysis. enzymatic activity assay showed that the enzyme has maximum activity at ph 9.0 and relative activity less than 10% at ph 7.0. differential scanning calorimetry and circular dich ... | 2006 | 16532449 |
| structure of full-length bacterial chitinase containing two fibronectin type iii domains revealed by small angle x-ray scattering. | chitinase a1 (chia1) from bacillus circulans wl-12 consists of an n-terminal catalytic domain, two fibronectin type iii domains (fniiids), and a c-terminal chitin-binding domain. the full-length structure of chia1 was studied by small angle x-ray scattering. the obtained low-resolution structure showed that chia1 is an elongated molecule with a length of approximately 145 a composed of a large globular head and a rod-like tail. combination with known high-resolution structures of individual chia ... | 2006 | 16899221 |
| mapping of residues involved in the interaction between the bacillus subtilis xylanase a and proteinaceous wheat xylanase inhibitors. | the bacillus subtilis xylanase a was subjected to site-directed mutagenesis, aimed at changing the interaction with triticum aestivum xylanase inhibitor, the only wheat endogenous proteinaceous xylanase inhibitor interacting with this xylanase. the published structure of bacillus circulans xyna was used to target amino acids surrounding the active site cleft of b.subtilis xyna for mutation. twenty-two residues were mutated, resulting in 62 different variants. the catalytic activity of active mut ... | 2006 | 16517552 |
| beneficiation of iron ore slime using aspergillus niger and bacillus circulans. | studies were carried out on the removal of alumina from iron ore slime containing (%) fe(2)o(3) 75.7, al(2)o(3) 9.95, sio(2) 6.1, fe (total) 52.94 with the help of bacillus circulans and aspergillus niger. b. circulans and a. niger showed 39% and 38% alumina removal after six and 15 days of in situ leaching at 10% pulp density, respectively. culture filtrate leaching with a. niger removed 20% alumina at 2% pulp density with 13 day old culture filtrate. b. circulans was more efficient than a. nig ... | 2006 | 16531043 |
| purification, characterization, and gene analysis of cellulase (cel8a) from lysobacter sp. ib-9374. | an enzyme that has both beta-1,4-glucanase and chitosanase activities was found in the culture medium of the soil bacterium lysobacter sp. ib-9374, a high lysyl endopeptidase-producing strain. the enzyme was purified to homogeneity from the culture filtrate using five purification steps and designated cel8a. the purified cel8a had a molecular mass of 41 kda, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. a ph optimum of 5.0 was found for the beta-1,4-glucanase activit ... | 2006 | 17031054 |
| cloning, sequencing, and expression of the genes encoding an isocyclomaltooligosaccharide glucanotransferase and an alpha-amylase from a bacillus circulans strain. | the gene for a novel glucanotransferase, isocyclomaltooligosaccharide glucanotransferase (igty), involved in the synthesis of a cyclomaltopentaose cyclized by an alpha-1,6-linkage [icg5; cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}] from starch, was cloned from the genome of b. circulans am7. the igty gene, designated igty, consisted of 2,985 bp encoding a signal peptide of 35 amino acids and a mature protein of 960 amino ac ... | 2006 | 17090949 |
| production of isocyclomaltopentaose from starch using isocyclomaltooligosaccharide glucanotransferase. | production of a novel cyclomaltopentaose cyclized by an alpha-1,6-linkage, [icg5; cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}], from starch was performed using isocyclomaltooligosaccharide glucanotransferase (igtase) derived from bacillus circulans am7. the optimal conditions for icg5-production from partially hydrolyzed starch were as follows: substrate concentration, 1.0% (w/v); ph, 5.5; temperature, 45 degrees c; reactio ... | 2006 | 17151467 |
| identification of catalytic amino acids of cyclodextran glucanotransferase from bacillus circulans t-3040. | in glycoside hydrolase family 66 (see http://afmb.cnrs-mrs.fr/cazy/), cyclodextran glucanotransferase (citase) is the only transglycosylation enzyme, all the other family 66 enzymes being dextranases. to analyze the catalytic amino acids of citase, we modified citase chemically from the t-3040 strain of bacillus circulans with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (edc). edc inactivated the enzyme by following pseudo-first order kinetics. in addition, the substrates of an isomaltooligos ... | 2006 | 16926507 |
| a novel glucanotransferase from a bacillus circulans strain that produces a cyclomaltopentaose cyclized by an alpha-1,6-linkage. | a novel glucanotransferase, involved in the synthesis of a cyclomaltopentaose cyclized by an alpha-1,6-linkage [icg5; cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}], from starch, was purified to homogeneity from the culture supernatant of bacillus circulans am7. the pi was estimated to be 7.5. the molecular mass of the enzyme was estimated to be 184 kda by gel filtration and 106 kda by sds-page. these results suggest that the ... | 2006 | 16926508 |
| bacteriocins reduce campylobacter colonization and alter gut morphology in turkey poults. | campylobacter is a leading cause of food-borne illness in the united states. recent evidence has demonstrated that bacteriocins produced by bacillus circulans and paenibacillus polymyxa reduce cecal campylobacter colonization in broiler chickens infected with campylobacter jejuni. as campylobacter coli is the most prevalent campylobacter isolate recovered in turkeys, the objectives of the present study were to evaluate the efficacy of these bacteriocins against c. coli colonization and their inf ... | 2006 | 16977842 |
| anti-apoptotic activity of laminarin polysaccharides and their enzymatically hydrolyzed oligosaccharides from laminaria japonica. | laminarin polysaccharides (lp1) were prepared from laminaria japonica, a marine brown alga with potential biological activities, by hot water extraction, ultrafiltration and gel chromatography; the molecular weights of the lp1s were between 5 and 10 kda. laminarin oligosaccharides (lo) derived by hydrolyzing lp1 with an endo-beta-(1-->3)-glucanase from bacillus circulans were mainly di- and penta-oligosaccharides. treatment of mouse thymocytes with lo or lp1 (1-4 mg ml(-1)) suppressed apoptotic ... | 2006 | 16614911 |
| bacillus anthracis virulent plasmid px02 genes found in large plasmids of two other bacillus species. | in order to cause the disease anthrax, bacillus anthracis requires two plasmids, px01 and px02, which carry toxin and capsule genes, respectively, that are used as genetic targets in the laboratory detection of the bacterium. clinical, forensic, and environmental samples that test positive by pcr protocols established by the centers for disease control and prevention for b. anthracis are considered to be potentially b. anthracis until confirmed by culture and a secondary battery of tests. we rep ... | 2006 | 16825351 |
| an enzymatically produced novel cyclomaltopentaose cyclized from amylose by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}. | a bacterial strain am7, isolated from soil and identified as bacillus circulans, produced two kinds of novel cyclic oligosaccharides. the cyclic oligosaccharides were produced from amylose using a culture supernatant of the strain as the enzyme preparation. the major product was a cyclomaltopentaose cyclized by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}. the other minor product was cyclomaltohexao ... | 2006 | 16545346 |
| characterisation of the microflora of attiéké, a fermented cassava product, during traditional small-scale preparation. | attiéké is a fermented cassava product consumed mainly in cote d'ivoire. the aim of this study was to characterise the attiéké fermentation by examining products from 15 small-scale production sites at various stages of its preparation. for the preparation of attiéké, fresh cassava is grated to a pulp and inoculated with 10% of a spontaneous traditional inoculum. the inocula contained aerobic mesophiles at mean numbers of 8.2 x 10(7) cfu/g and lactic and acetic acids at mean concentrations of 0. ... | 2006 | 16213052 |
| crystallization and preliminary x-ray analysis of the catalytic domain of chitinase d from bacillus circulans wl-12. | we report here on crystallization and preliminary x-ray analysis of the catalytic domain of chitinase d from bacillus circulans wl-12. the native crystals of this domain were found to belong to the orthorhombic space group p2(1)2(1)2(1). to elucidate the structure of the catalytic domain by the multiple isomorphous replacement method, 30 kinds of derivatized crystals were prepared by soaking the native crystals into a mother liquor containing salts of heavy metal atoms. difference patterson maps ... | 2006 | 17100652 |
| statistical optimization of thermo-tolerant xylanase activity from amazon isolated bacillus circulans on solid-state cultivation. | a 2(2) factorial design was performed to find the best conditions of ph and temperature for xylanolytic activity of bacillus circulans bl53 isolated from the amazon environment. solid-state cultivation was carried out on an inexpensive, abundant agro-industrial soybean residue. the central composite design (ccd) used for the analysis of treatment combinations showed that a second-order polynomial regression model was in good agreement with experimental results, with r(2) = 0.9369 (p < 0.05). the ... | 2006 | 16216495 |
| role of glutamate 243 in the active site of 2-deoxy-scyllo-inosose synthase from bacillus circulans. | 2-deoxy-scyllo-inosose (doi) synthase is involved in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics and catalyzes the carbocyclic formation from d-glucose-6-phosphate (g-6-p) into doi. the reaction mechanism is proposed to be similar to that of dehydroquinate (dhq) synthase in the shikimate pathway, and includes oxidation of c-4, beta-elimination of phosphate, reduction of c-4, ring opening, and intramolecular aldol cyclization. to investigate the reaction mechanism ... | 2007 | 17035031 |
| cyclodextrin production by cyclodextrin glycosyltransferase from bacillus circulans df 9r. | cyclodextrins (cd) are cyclic oligosaccharides with multiple applications in the food, pharmaceutical, cosmetic, agricultural and chemical industries. in this work, the conditions used to produce cd with cyclodextrin glycosyltransferase from bacillus circulans df 9r were optimized using experimental designs. the developed method allowed the partial purification and concentration of the enzyme from the cultural broth and, subsequently, the cd production, using the same cassava starch as enzyme ad ... | 2007 | 17174549 |
| bacillus circulans wz-12 - a newly discovered aerobic dichloromethane-degrading methylotrophic bacterium. | a novel dichloromethane (dcm)-degrading bacterial strain named wz-12 (genbank accession no. ef100968) was isolated and identified as bacillus circulans based on standard morphological and physiological properties and nucleotide sequence analysis of enzymatically amplified 16s ribosomal deoxyribonucleic acid. dcm dehalogenase from b. circulans wz-12 was purified to 8.27-fold with a yield of 34.83%. the electrophoretically homogeneous-purified enzyme exhibited a specific activity of 118.82 u/mg. s ... | 2007 | 17687552 |
| elaboration of neosamine rings in the biosynthesis of neomycin and butirosin. | the proteins neo-11 and neo-18 encoded in the neomycin gene cluster (neo) of streptomyces fradiae ncimb 8233 have been characterized as glucosaminyl-6'-oxidase and 6'-oxoglucosaminyl:l-glutamate aminotransferase, respectively. the joint activity of neo-11 and neo-18 is responsible for the conversion of paromamine to neamine in the biosynthetic pathway of neomycin through a mechanism of fad-dependent dehydrogenation followed by a pyridoxal-5'-phosphate-mediated transamination. neo-18 is also show ... | 2007 | 17206729 |
| characterization of the enzyme btrd from bacillus circulans and revision of its functional assignment in the biosynthesis of butirosin. | 2007 | 17226887 | |
| mechanism of stabilization of bacillus circulans xylanase upon the introduction of disulfide bonds. | the introduction of disulfide bonds has been used as a strategy to enhance the stability of bacillus circulans xylanase. the transition temperature of the s100c/n148c (ds1), v98c/a152c (ds2), and a1gc/g187,c188 (cxl) in comparison to the wild type was increased by 5.0, 4.1 and 3.8 degrees c, respectively. interestingly, a combination of two disulfide bonds of ds1 and cxl (cds1, circular disulfide 1) led to a 12 degrees c increase in the transition temperature. importantly, an increase in the mel ... | 2007 | 17141401 |
| effect on epidermal cell of soybean protein-degraded products and structural determination of the root hair promoting peptide. | peptide(s) produced from degraded soybean protein by an alkaline protease from bacillus circulans ha12 (degraded soybean-meal products; dsp) increased the number of both the root hair cells (trichoblasts) and hairless cells (atrichoblasts) of brassica rapa by about 4.4 times and 1.9 times, respectively. to identify the root hair-promoting peptide(s) in dsp, the origin protein of the root hair-promoting peptide(s) was identified as kunitz trypsin inhibitor (kti). the root hair-promoting peptide i ... | 2007 | 17671783 |
| recruitment of both uniform and differential binding energy in enzymatic catalysis: xylanases from families 10 and 11. | the contributions of enzyme-substrate hydrogen-binding interactions to catalysis by two different families of xylanases were evaluated through kinetic studies with two representative wild-type enzymes, cellulomonas fimi xylanase (cex) and bacillus circulans xylanase (bcx), on a series of monodeoxygenated and monodeoxyfluorinated p-nitrophenyl xylobioside substrates. effects of substitution in the distal (-2 subsite) sugar on kcat/km for cex were moderately large (up to 2.9 kcal mol-1), with no e ... | 2007 | 17503782 |
| terpenoids and flavonoids from laggera pterodonta. | to study the chemical constituents of aerial parts of laggera pterodonta (dc.) benth., the air-dried aerial parts of this plant were powered and extracted with boiling water and purified by silica gel column chromatography and recrystallized. eleven compounds were obtained from l. pterodonta. they were identified as to be 6-o-beta-d-glucopyranosyl-carvotanacetone (1), pterodontic acid (2), 1beta-hydroxy pterondontic acid (3), pterodontoside a (4), pterodondiol (5), pterodontriol b (6), 5-hydroxy ... | 2007 | 17703774 |
| efficient production of 2-deoxy-scyllo-inosose from d-glucose by metabolically engineered recombinant escherichia coli. | 2-deoxy-scyllo-inosose (doi) is a six-membered carbocycle formed from d-glucose-6-phosphate catalyzed by 2-deoxy-scyllo-inosose synthase (dois), a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics. doi is valuable as a starting material for the benzene-free synthesis of catechol and other benzenoids. we constructed a series of metabolically engineered escherichia coli strains by introducing a dois gene (btrc) from bacillus circulans and disrupting genes fo ... | 2007 | 17368605 |
| accurate, conformation-dependent predictions of solvent effects on protein ionization constants. | predicting how aqueous solvent modulates the conformational transitions and influences the pka values that regulate the biological functions of biomolecules remains an unsolved challenge. to address this problem, we developed fdpb_mf, a rotamer repacking method that exhaustively samples side chain conformational space and rigorously calculates multibody protein-solvent interactions. fdpb_mf predicts the effects on pka values of various solvent exposures, large ionic strength variations, strong e ... | 2007 | 17360348 |
| a novel cyclic isomaltooligosaccharide (cycloisomaltodecaose, ci-10) produced by bacillus circulans t-3040 displays remarkable inclusion ability compared with cyclodextrins. | cyclodextrans (cis) are cyclic isomaltooligosaccharides and only ci-7, ci-8, and ci-9 were known. ci-7, ci-8, and ci-9, consisting of seven, eight, and nine glucoses, respectively, bound by alpha-(1-->6) linkages, are known to be produced by t-3040 strain of bacillus circulans. however, we have found, using 13c nmr and mass spectrometry, that this strain also produces ci-10, ci-11 and ci-12. these large cis are very soluble in water and inhibit the glucan synthesis of glucansucrases to the same ... | 2007 | 17433485 |
| biosynthesis of butirosin: transfer and deprotection of the unique amino acid side chain. | butirosin, an aminoglycoside antibiotic produced by bacillus circulans, bears the unique (s)-4-amino-2-hydroxybutyrate (ahba) side chain, which protects the antibiotic from several common resistance mechanisms. the ahba side chain is advantageously incorporated into clinically valuable antibiotics such as amikacin and arbekacin by synthetic methods. therefore, it is of significant interest to explore the biosynthetic origins of this useful moiety. we report here that the ahba side chain of butir ... | 2007 | 17462573 |
| unique o-ribosylation in the biosynthesis of butirosin. | using a comparative genetics approach, one or more of the btra, btrl, btrp, and btrv proteins encoded in the butirosin biosynthetic gene cluster (btr) from bacillus circulans sank72073 were identified as being responsible for an o-ribosylation process leading to the formation of ribostamycin, a key intermediate in this, and related antibiotic biosynthetic pathways. functional analysis of the recombinantly expressed proteins revealed that both btrl and btrp were responsible for the ribosylation o ... | 2007 | 17482823 |
| biological pretreatment with two bacterial strains for enzymatic hydrolysis of office paper. | the cellulose-hydrolyzing strains, sphingomonas paucimobilis mk1 and bacillus circulans mk2, were separated from soil and were grown together in a single culture plate. growth b. circulans mk2 in liquid culture required symbiosis with s. paucimobilis mk1. biological pretreatment with the combined strain suspension after the liquid culture improved enzymatic hydrolysis of office paper from municipal wastes. sugar recovery by s. paucimobilis mk1 (51%) was 1.4 times higher than that of the untreate ... | 2007 | 17487532 |
| a secondary xylan-binding site enhances the catalytic activity of a single-domain family 11 glycoside hydrolase. | bacillus circulans xylanase (bcx) is a single-domain family 11 glycoside hydrolase. using nmr-monitored titrations, we discovered that an inactive variant of this enzyme, e78q-bcx, bound xylooligosaccharides not only within its pronounced active site (as) cleft, but also at a distal surface region. chemical shift perturbation mapping and affinity electrophoresis, combined with mutational studies, identified the xylan-specific secondary binding site (sbs) as a shallow groove lined by asn, ser, an ... | 2007 | 17822716 |
| production of cyclodextrin glycosyltransferase by alkaliphilic bacillus circulans in submerged and solid-state cultivation. | cyclodextrin glycosyltransferase (cgtase; e.c. 2.4.1.19) is an industrially important enzyme, which is used to produce cyclodextrins (cds). in this research, we report the use of experimental factorial design to find the best conditions of ph and temperature for cgtase production by bacillus circulans var. alkalophilus. the optimized calculated values for the tested variables were, respectively, ph 9.7 and temperature 36 degrees c, with a cgtase activity of 615 u ml(-1). the cgtase production wa ... | 2007 | 17574546 |
| mutanase from a paenibacillus isolate: nucleotide sequence of the gene and properties of recombinant enzymes. | a mutanase (alpha-1,3-glucanase)-producing microorganism was isolated from a soil sample and was identified as a relative of paenibacillus sp. the mutanase was purified to homogeneity from culture, and its molecular mass was around 57 kda. the gene for the mutanase was cloned by pcr using primers based on the n-terminal amino acid sequence of the purified enzyme. the determined nucleotide sequence of the gene consisted of 3651-bp open reading frame that encoded a predicted 1217-amino acid polype ... | 2007 | 17270351 |
| competitive inhibition bacteria of bovine origin against salmonella serovars. | studies were conducted to isolate bacteria inhibitory to salmonella enterica serovar typhimurium definitive type (dt) 104 in vitro from cattle not carrying salmonella and to determine the inhibitory activity of the isolated bacteria through competitive growth in cattle feces artificially contaminated with salmonella typhimurium dt104 and s. enterica serovar newport. fecal samples (108) were obtained from dairy and beef cows. s. enterica serovars were isolated from 9.25% of the samples and includ ... | 2007 | 17803135 |
| characterization and mechanistic study of a radical sam dehydrogenase in the biosynthesis of butirosin. | btrn encoded in the butirosin biosynthetic gene cluster possesses a cxxxcxxc motif conserved within the radical s-adenosyl methionine (sam) superfamily. its gene disruption in the butirosin producer bacillus circulans caused the interruption of the biosynthetic pathway between 2-deoxy-scyllo-inosamine (doia) and 2-deoxystreptamine (dos). further, in vitro assay of the overexpressed enzyme revealed that btrn catalyzed the oxidation of doia under the strictly anaerobic conditions along with consum ... | 2007 | 18001019 |
| characterization of nocardiopsis beta-1,3-glucanase with additional carbohydrate-binding domains. | beta-1,3-glucanase f (bglf) from alkaliphilic nocardiopsis sp. f96 is a single domain enzyme composed of only a catalytic domain. chimeric bglfs with some carbohydrate-binding domains were constructed and characterized. by connecting the c-terminal additional domain of beta-1,3-glucanase h from bacillus circulans iam1165 and the chitin-binding domain of chitinase j from alkaliphilic bacillus sp. j813, binding ability and hydrolyzing activity toward insoluble beta-1,3-glucans were both improved. | 2007 | 18029785 |
| antagonistic and antimicrobial activities of some bacterial isolates collected from soil samples. | thirty seven bacterial cultures isolated from soil samples obtained from different locations were tested for their antagonistic activity against some fungal pathogens, viz., sclerotium rolfsii, fusarium oxysporum and rhizoctonia solani, causal agents of collar rot of sunflower, wilts and root rots, respectively. among them, 5 bacterial strains, viz., a1 6 (bacillus sphaericus), k1 24 (pseudomonas fluorescens), m1 42 (bacillus circulans), m1 66 (bacillus brevis) and t1 22 (bacillus brevis) showed ... | 2007 | 23100644 |
| an alkaline protease from bacillus circulans bm15, newly isolated from a mangrove station: characterization and application in laundry detergent formulations. | an investigation on the properties of an alkaline protease secreted by bacillus circulans bm15 strain isolated from a mangrove sediment sample was carried out in order to characterize the enzyme and to test its potency as a detergent additive. the protease was purified to apparent homogeneity by ammonium sulphate precipitation and was a 30-kda protease as shown by sds-page and its proteolytic activity was detected by casein zymography. it had optimum activity at ph 7, was stable at alkaline ph r ... | 2007 | 23100681 |
| thermal and chemical denaturation of bacillus circulans xylanase: a biophysical chemistry laboratory module. | a number of institutions have been, or are in the process of, modifying their biochemistry major to include some emphasis on the quantitative physical chemistry of biomolecules. sometimes this is done as a replacement for part for the entire physical chemistry requirement, while at other institutions this is incorporated as a component into the traditional two-semester biochemistry series. the latter is the model used for biochemistry and molecular biology majors at the university of richmond, w ... | 2008 | 21591232 |
| shifting ph optimum of bacillus circulans xylanase based on molecular modeling. | although hydrolases are used in several industrial processes, its industrial applications have some limitations in specific cases since some industrial processes are carried out at ph value which is different from optimum ph of the enzyme. alkaline side optimum ph of hydrolases is always desirable, and it is proved difficult to achieve that by mutation only. hence, molecular modeling was applied to select the promising mutants. the changes in electrostatic potential, which was calculated using d ... | 2008 | 18077046 |
| purification and characterization of chitinase a of streptomyces cyaneus sp-27: an enzyme participates in protoplast formation from schizophyllum commune mycelia. | a culture filtrate of bacillus circulans ka-304 grown on a cell-wall preparation of schizophyllum commune has an activity to form protoplasts from s. commune mycelia. alpha-1,3-glucanase and chitinase i, which were isolated from the filtrate, did not form the protoplast by itself while a mixture of them showed protoplast-forming activity. streptomyces cyaneus sp-27 was isolated based on the productivity of chitinase. the culture filtrate of s. cyaneus sp-27 did not form s. commune protoplasts, b ... | 2008 | 18175902 |
| xylanase production by bacillus circulans d1 using maltose as carbon source. | bacillus circulans d1 is a good producer of extracellular thermostable xylanase. xylanase production in different carbon sources was evaluated and the enzyme synthesis was induced by various carbon sources. it was found that d-maltose is the best inducer of the enzyme synthesis (7.05 u/mg dry biomass at 48 h), while d-glucose and d-arabinose lead to the production of basal levels of xylanase. the crude enzyme solution is free of cellulases, even when the microorganism was cultivated in a medium ... | 2008 | 18421584 |
| studies on improving the immobilized bead reusability and alkaline protease production by isolated immobilized bacillus circulans (mtcc 6811) using overall evaluation criteria. | this study uses an overall evaluation criterion for improving the immobilized bead reusability and extracellular enzyme production by immobilized cells by assigning relative weightage to bead reusability, enzyme production, and cell leakage. initially, alkaline protease production by alginate-immobilized bacillus circulans (mtcc 6811) was analyzed using l18 orthogonal array (oa). the resultant optimized parameters were further fine-tuned with l9 oa experimentation. at l18-oa analysis, inoculum l ... | 2008 | 18568299 |
| cloning and expression of chitinase a gene from streptomyces cyaneus sp-27: the enzyme participates in protoplast formation of schizophyllum commune. | chitinase a of streptomyces cyaneus sp-27 or chitinase i of bacillus circulans ka-304 showed the protoplast-forming activity when combined with alpha-1,3-glucanase of b. circulans ka-304. the gene of chitinase a was cloned. it consisted of 903 nucleotides encoding 301 amino acid residues, including a putative signal peptide (35 amino acid residues). the deduced n-terminal moiety of chitinase a showed sequence homology with the chitin-binding domain of chitinase f from streptomyces coelicolor and ... | 2008 | 18603792 |
| chemoenzymatic acylation of aminoglycoside antibiotics. | the chemoenzymatic installation of the clinically valuable (s)-4-amino-2-hydroxybutyryl side chain onto a number of 2-deoxystreptamine-containing aminoglycosides is described using the purified bacillus circulans biosynthetic enzymes btrh and btrg in combination with a synthetic acyl-snac surrogate substrate. | 2008 | 18685777 |
| [influence of different sources of carbon and nitrogen on the biosynthesis of proteolytic complexes by bacillus circulans 693, bacillus sp. 27 and yarrowia lipolytica 2061 strains]. | the influence of different factors on the biosynthesis of extracellular proteolytic complexes by strains-producers bacillus circulans 693, bacillus sp. 27 and yarrowia lipolytica 2061 at submerged cultivation has been investigated. it has been shown that ammonium hydrocarbonate and gelatin with glucose were optimum carbon and nitrogen sources for synthesis of proteolytic activity of b. circulans strain 693, gelatin with arabinose--for bacillus sp. 27, gelatin and glycine with sorbose--for y. lip ... | 2008 | 18959038 |
| bacillus circulans paracardiac infection in non-hodgkin lymphoma--a case report. | a case report is presented concerning bacillus circulans paracardiac infection in a 27 year old woman with non-hodgkin lymphoma. | 2008 | 19097386 |
| the cyclodextrin glycosyltransferase of paenibacillus pabuli us132 strain: molecular characterization and overproduction of the recombinant enzyme. | the gene encoding the cyclodextrin glycosyltransferase (cgtase) of paenibacillus pabuli us132, previously described as efficient antistaling agent and good candidate for cyclodextrins production, was cloned, sequenced, and expressed in escherichia coli. sequence analysis showed that the mature enzyme (684 amino acids) was preceded by a signal peptide of 34 residues. the enzyme exhibited the highest identity (94%) to the beta-cgtase of bacillus circulans no. 8. the production of the recombinant c ... | 2008 | 18704190 |
| determination of electrostatic interaction energies and protonation state populations in enzyme active sites. | the ph-dependence of the nmr chemical shift for titratable groups in proteins often deviate from a standard henderson-hasselbalch (hh) titration curve. a non-hh dependence of the chemical shift for a given residue can arise from a single-site, non-hh titrational event for that residue, or if the chemical shift of the group is influenced by additional titrational events occurring in other residues. we show that simultaneous fits of several non-hh nmr titration curves of interacting protein residu ... | 2008 | 18155242 |
| structural simulation and protein engineering to convert an endo-chitosanase to an exo-chitosanase. | to obtain an enzyme for the production of chito-disaccharides (glcn(2)) by converting endo-chitosanase to exo-chitosanase, we chose an endo-chitosanase from bacillus circulans mh-k1 (csn) as the candidate for protein engineering. using molecular modeling, two peptides with five amino acids (pclgg) and six amino acids (srtckp) were designed and inserted after the positions of d(115) and t(222) of csn, respectively. the inserted fragments are expected to form loops that might protrude from opposit ... | 2008 | 18540010 |
| modelling and optimization of fermentation factors for enhancement of alkaline protease production by isolated bacillus circulans using feed-forward neural network and genetic algorithm. | modelling and optimization of fermentation factors and evaluation for enhanced alkaline protease production by bacillus circulans. | 2008 | 17953681 |
| structure of 2-deoxy-scyllo-inosose synthase, a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics, in complex with a mechanism-based inhibitor and nad+. | a key enzyme in the biosynthesis of clinically important aminoglycoside antibiotics is 2-deoxy-scyllo-inosose synthase (dois), which catalyzes carbocycle formation from d-glucose-6-phosphate to 2-deoxy-scyllo-inosose through a multistep reaction. this reaction mechanism is similar to the catalysis by dehydroquinate synthase (dhqs) of the cyclization of 3-deoxy-d-arabino-heputulosonate-7-phosphate to dehydroquinate in the shikimate pathway, but significant dissimilarity between these enzymes is a ... | 2008 | 17879343 |
| use of metabolic and molecular methods for the identification of a bacillus strain isolated from paper affected by foxing. | foxing of paper is a deterioration phenomenon occurring in the form of brown-yellowish spots, the abiotic and/or biotic causes of which are not yet completely understood. nevertheless, microbiological infection has been recognized that may contribute to paper damage and therefore it becomes important to know the taxonomic position and the degradative activity of the potential infectious biological agents which mostly are fungi, but also bacteria and yeasts. a cellulolytic bacterial strain isolat ... | 2008 | 17686620 |
| decolorization of azo dyes and simulated dye bath wastewater using acclimatized microbial consortium--biostimulation and halo tolerance. | anaerobic acclimatization of activated sludge from a textile effluent treatment plant to high concentration of rb5 could effectively decolorize rb5 dye solution. the strains viz. pseudomonas aeruginosa and bacillus circulans and other unidentified laboratory isolates (nad1 and nad6) were predominantly present in the microbial consortium. the conditions for efficient decolorization, biostimulation to increase effectiveness of microbial consortium, its tolerance to high salt concentration and non- ... | 2008 | 17566726 |
| purification and immobilization of recombinant variants of brevundimonas diminuta glutaryl-7-aminocephalosporanic acid acylase expressed in escherichia coli cells. | modified chitin-binding domain (chbd) from bacillus circulans chitinase a1 with w42f mutation in chitin-binding site was genetically fused to different positions within alpha-subunit of glutaryl-7-aminocephalosporanic acid acylase (gla) gene. hybrid proteins were efficiently expressed in e. coli cells as soluble, enzymatically active and correctly processed holoenzymes. chbd-gla fusions were easily affinity purified on chitin column by changing the salt concentration of binding and elution buffe ... | 2008 | 17963935 |
| improved bioavailability and biodegradation of a model polyaromatic hydrocarbon by a biosurfactant producing bacterium of marine origin. | polyaromatic hydrocarbons (pahs) are organic pollutants mostly derived from the processing and combustion of fossil fuels and cause human health hazards. in the present study a marine biosurfactant producing strain of bacillus circulans was used to increase the bioavailability and consequent degradation of a model polyaromatic hydrocarbon, anthracene. although the organism could not utilize anthracene as the sole carbon source, it showed better growth and biosurfactant production in an anthracen ... | 2008 | 18565569 |
| antimicrobial potential of a lipopeptide biosurfactant derived from a marine bacillus circulans. | to isolate the biologically active fraction of the lipopeptide biosurfactant produced by a marine bacillus circulans and study its antimicrobial potentials. | 2008 | 18194244 |
| gene cloning, expression, and characterization of a novel beta-mannanase from bacillus circulans cgmcc 1416. | a dna fragment containing 2,079 base pairs from bacillus circulans cgmcc 1416 was cloned using degenerate pcr and inverse pcr. an open reading frame containing 981 bp was identified that encoded 326 amino acids residues, including a putative signal peptide of 31 residues. the deduced amino acid sequence showed the highest identity (68.1%) with endo-beta-1,4-d-mannanase from bacillus circulans strain k-1 of the glycoside hydrolase family 5 (gh5). the sequence encoding the mature protein was clone ... | 2008 | 18239434 |
| synthesis of a series of fructooligosaccharides with sucrose and cycloinulohexaose extending over ten degrees of polymerization using cycloinulooligosaccharide fructanotransferase from bacillus circulans okumz 31b. | prolonged incubation with sucrose as an acceptor and cycloinulohexaose as a donor at a high concentration of cycloinulooligosaccharide fructanotransferase afforded a series of fructooligosaccharides. their chain-length distribution extended over 10 degrees of polymerization when the donor concentration was increased to 120 mm. increasing the acceptor concentration proved effective in improving the yield of inulin-type oligosaccharides because hydrolysis was suppressed. | 2008 | 18391455 |
| mechanistic study on the reaction of a radical sam dehydrogenase btrn by electron paramagnetic resonance spectroscopy. | btrn is a radical sam ( s-adenosyl- l-methionine) enzyme that catalyzes the oxidation of 2-deoxy- scyllo-inosamine (doia) into 3-amino-2,3-dideoxy- scyllo-inosose (amino-doi) during the biosynthesis of 2-deoxystreptamine (dos) in the butirosin producer bacillus circulans. recently, we have shown that btrn catalyzes the transfer of a hydrogen atom at c-3 of doia to 5'-deoxyadenosine, and thus, the reaction was proposed to proceed through the hydrogen atom abstraction by the 5'-deoxyadenosyl radic ... | 2008 | 18672902 |
| solid state bioreactor production of transglutaminase by amazonian bacillus circulans bl32 strain. | in this work, we investigated the production of transglutaminase (tgase) by an amazonian isolated strain of bacillus circulans by solid-state cultivation (ssc). several agro-industrial residues, such as untreated corn grits, milled brewers rice, industrial fibrous soy residue, soy hull, and malt bagasse, were used as substrates for microbial growth and enzyme production. growth on industrial fibrous soy residue, which is rich in protein and hemicellulose, produced the highest tgase activity (0.7 ... | 2008 | 18696133 |
| comparative analysis of the diversity of aerobic spore-forming bacteria in raw milk from organic and conventional dairy farms. | bacterial contamination of raw milk can originate from different sources: air, milking equipment, feed, soil, faeces and grass. it is hypothesized that differences in feeding and housing strategies of cows may influence the microbial quality of milk. this assumption was investigated through comparison of the aerobic spore-forming flora in milk from organic and conventional dairy farms. laboratory pasteurized milk samples from five conventional and five organic dairy farms, sampled in late summer ... | 2008 | 18406093 |
| nucleotide and deduced amino acid sequences of mutanase-like genes from paenibacillus isolates: proposal of a new family of glycoside hydrolases. | three mutanase (alpha-1,3-glucanase)-producing microorganisms isolated from soil samples were identified as a relatives of paenibacillus. a mutanase was purified to homogeneity from cultures of each, and the molecular masses of the purified enzymes were approximately 132, 141, and 141kda, respectively. the corresponding three genes for mutanases were cloned by pcr using primers designed from each n-terminal amino acid sequence. another mutanase-like gene from one strain was also cloned by pcr us ... | 2008 | 17988780 |
| in vitro antimicrobial effect of satureja wiedemanniana against bacillus species isolated from raw meat samples. | in this study a total of 30 raw meat samples obtained from ankara, turkey were screened for the presence of bacillus species. among the meat samples analyzed, the predominant species isolated was bacillus circulans; other bacillus species were identified as bacillus firmus, bacillus lentus, bacillus megaterium, bacillus licheniformis, bacillus mycoides, bacillus sphaericus, and bacillus cereus. minced meat samples were more contaminated with bacillus species than sliced beef sample. from these s ... | 2009 | 19735196 |
| analysis of the involvement of chitin-binding domain of chicw in antifungal activity, and engineering a novel chimeric chitinase with high enzyme and antifungal activities. | an antifungal chitinase, chicw, produced by bacillus cereus 28-9 is effective against conidial germination of botrytis elliptica, the causal agent of lily leaf blight. chicw as a modular enzyme consists of a signal peptide, a catalytic domain, a fibronectin type-iii-like domain, and a chitin-binding domain. when two c-terminal domains of chicw were truncated, chicwdeltafc (lacking the chitin-binding domain and fibronectin type iii-like domain) lost its antifungal activity. since chicwdeltaac (la ... | 2009 | 19884776 |
| [biosynthesis of secreted ribonucleases by bacillus intermedius and bacillus circulans during nitrogen starvation]. | 2009 | 19449735 | |
| enhancement of l-asparaginase production by isolated bacillus circulans (mtcc 8574) using response surface methodology. | l-asparaginase production was optimized using isolated bacillus circulans (mtcc 8574) under solid-state fermentation (ssf) using locally available agricultural waste materials. among different agricultural materials (red gram husk, bengal gram husk, coconut, and groundnut cake), red gram husk gave the maximum enzyme production. a wide range of ssf parameters were optimized for maximize the production of l-asparaginase. preliminary studies revealed that incubation temperature, moisture content, i ... | 2009 | 19052920 |
| a novel beta-mannanase with high specific activity from bacillus circulans cgmcc1554: gene cloning, expression and enzymatic characterization. | a dna fragment of 2,042 bp containing a novel beta-mannanase gene, man5a, was identified from the genome of the mannan-degrading bacterium bacillus circulans cgmcc1554. the open reading frame of man5a comprised 978 bp encoding a protein of 326 amino acids with a predicted molecular weight of 32 kda. the amino acid sequence of the encoded mannanase, man5a, showed the highest identity (78.5%) to beta-mannanases belonging to glycosyl hydrolases family 5. the gene man5a was efficiently expressed in ... | 2009 | 18820840 |
| structure-function relationship in cyclodextrin glycosyltransferase from bacillus circulans df 9r. | cyclodextrin glycosyltransferases (cgtases e.c.2.4.1.19) catalyze cyclomaltooligosaccharides (cyclodextrins) production, an important industrial process. we herein report structural features of bacillus circulans df 9r cyclodextrin glycosyltransferase including its sequence and several aspects of enzyme structure-function relationship. protein ethoxyformylation, under our experimental conditions, indicated that only one out of the 13 enzyme histidines was modified leading to a drastic drop in cy ... | 2009 | 18922514 |
| identification and cloning of a gene encoding dichloromethane dehalogenase from a methylotrophic bacterium, bacillus circulans wz-12 cctcc m 207006. | the gene dehala encoding a novel dichloromethane dehalogenases (dehala), has been cloned from bacillus circulans wz-12 cctcc m 207006. the open reading frame of dehala, spanning 864 bp, encoded a 288-amino acid protein that showed 85.76% identity to the dichloromethane dehalogenases of hyphomicrobium sp. gj21 with several commonly conserved sequences. these sequences could not be found in putative dichloromethane (dcm) dehalogenases reported from other bacteria and fungi. dehala was expressed in ... | 2009 | 19277720 |
| effective enzymatic synthesis of lactosucrose and its analogues by beta-d-galactosidase from bacillus circulans. | in the present study, beta-d-galactosidase from bacillus circulans was proved to be a suitable biocatalyst for the production of lactosucrose (beta-d-galp-(1-->4)-alpha-d-glcp-(1-->2)-beta-d-fruf, i) and its analogues from lactose and sucrose. during the hydrolysis of lactose, the formation of four transfer products was followed by high performance liquid chromatography with refraction index detector. in addition, the transfer products were isolated from the reaction mixture and identified to be ... | 2009 | 19326862 |
| characterization of temperature dependent and substrate-binding cleft movements in bacillus circulans family 11 xylanase: a molecular dynamics investigation. | xylanases (ec 3.2.1.8) hydrolyze xylan, one of the most abundant plant polysaccharides found in nature, and have many potential applications in biotechnology. | 2009 | 19409448 |
| additional carbohydrate-binding modules enhance the insoluble substrate-hydrolytic activity of beta-1,3-glucanase from alkaliphilic nocardiopsis sp. f96. | beta-1,3-glucanase (bglf) from nocardiopsis sp. f96 is composed of only a catalytic domain. to improve the enzymatic properties of bglf, we attempted to construct chimeric enzymes consisting of bglf and some carbohydrate-binding modules, such as the c-terminal additional domain (cad) and the n-terminal additional domain (nad) of beta-1,3-glucanase h from bacillus circulans iam1165 and the chitin-binding domain (chbd) of chitinase from alkaliphilic bacillus sp. j813. cad-fused bglf (bglf-cad), na ... | 2009 | 19420727 |
| a robust method for the joint estimation of yield coefficients and kinetic parameters in bioprocess models. | bioprocess model structures that require nonlinear parameter estimation, thus initialization values, are often subject to poor identification performances because of the uncertainty on those initialization values. under some conditions on the model structure, it is possible to partially circumvent this problem by an appropriate decoupling of the linear part of the model from the nonlinear part of it. this article provides a procedure to be followed when these structural conditions are not satisf ... | 2009 | 19455623 |
| environmental effects on transglutaminase production and cell sporulation in submerged cultivation of bacillus circulans. | in this research, the effects of ph, temperature, and oxygen on growth kinetics of a newly isolated strain of bacillus circulans from the amazon and their correlations with transglutaminase (tgase) production and cell sporulation were investigated. statistical experimental methods were used to optimize these parameters, while induction of sporulation was achieved by oxygen culture control. full factorial composite experimental design and response surface methodology were experimentally tested. t ... | 2009 | 18716920 |
| effect of a probiotic bacterium bacillus circulans pb7 in the formulated diets: on growth, nutritional quality and immunity of catla catla (ham.). | bacillus circulans pb7, isolated from the intestine of catla catla, was evaluated for use as a probiotic supplement in the feeds for the fingerlings of catla catla. the effect of supplement on the growth performance, feed utilization efficiency, and immune response was evaluated. catla fingerlings (ave. wt. 6.48 +/- 0.43 g) were fed diets supplemented with 2 x 10(4) (feed c1), 2 x 10(5) (feed c2), and 2 x 10(6) (feed c3) b. circulans pb 7 cells per 100 g feed for 60 days at 5% of the body weight ... | 2009 | 18931930 |
| role of chitin binding domain of chitinase a of streptomyces cyaneus sp-27 in protoplast formation from schizophyllum commune. | chitinase a (chia) of streptomyces cyaneus sp-27 forms protoplasts from schizophyllum commune mycelia when it is combined with alpha-1,3-glucanase of bacillus circulans ka-304. an n-terminal chitin-binding domain truncated mutant (catchia), which was expressed in escherichia coli rosetta-gami b (de 3), lost most of its colloidal chitin- and powder chitin-binding activity. the colloidal chitin-hydrolyzing, the powder chitin-hydrolyzing, and the protoplast-forming activities of catchia were lower ... | 2009 | 19270417 |
| antimicrobial biosurfactants from marine bacillus circulans: extracellular synthesis and purification. | to purify the biosurfactant produced by a marine bacillus circulans strain and evaluate the improvement in surface and antimicrobial activities. | 2009 | 19187506 |
| identification and application of aflp-derived genetic markers for quantitative pcr-based tracking of bacillus and paenibacillus spp. released in soil. | in this study, we show that noncoding sequences from amplified fragment length polymorphisms (aflps) can provide robust and sensitive genetic markers suitable for pcr-based discrimination of closely related strains of bacillus and paenibacillus, and quantitative pcr (qpcr)-based tracking of the strains in complex natural systems like soil. quantitative pcr was accurate in the approximately 1 x 10(9) to approximately 1 x 10(4) colony forming units (cfu)/g soil range. the detection limit was impro ... | 2009 | 19935889 |
| [gene cloning and overexpression of dichloromethane dehalogenase from bacillus circulans wz-12]. | the dcmr gene encoding dichloromethane dehalogenase was amplified by pcr from bacillus circulans wz-12 and cloned to expression vector pet28b(+), yielding recombinant plasmid pet28b(+)-dcmr. then plasmid pet28b(+)-dcmr was introduced into escherichia. coli bl21(de3). expression was induced by iptg,and the enzyme activity reached 25.78 u/ml, the specific enzyme activity reached 88.86 u/mg protein.the periplasmic and cytoplasmic enzyme activity reached 2.92 u/ml and 22.86 u/ml respectively.all res ... | 2009 | 19799320 |
| facile pretreatment of bacillus circulans beta-galactosidase increases the yield of galactosyl oligosaccharides in milk and lactose reaction systems. | the commercially available preparation of beta-galactosidase from bacillus circulans , known as biolacta fn5, has been extensively used in the production of prebiotic galactooligosaccharides (gos). this study focuses on characterizing the production of gos in two reaction systems: 10% lactose (w/v) in buffer and skim milk. analysis of the temperature dependence of the gos yield along with the relative rates of gos synthesis and degradation leads to the finding that gos degradation activity was s ... | 2009 | 19921828 |
| rapid quantification of a microbial surfactant by a simple turbidometric method. | quantification of the biosurfactants produced by a variety of microorganisms is a time taking and difficult task due to the lack of rapid, efficient and accurate methods. this work presents a simple turbidometric method for quantification of crude biosurfactants based on their property to become insoluble at low ph values. biosurfactants obtained from a bacillus sp. using different carbon substrates showed a good linear correlation (r(2)>0.99) between biosurfactant concentrations and turbidity i ... | 2009 | 18840480 |
| thermostabilization of bacillus circulans xylanase via computational design of a flexible surface cavity. | despite recent advances in our understanding of the importance of protein-surface properties for protein thermostability, to date many rational designs have been focused instead on protein-core characteristics such as core packing and cavity filling. rational strategies to design protein surfaces to improve protein thermostability have not yet been well investigated. here, an efficient rational design of a surface cavity for improving protein thermostability without reducing enzyme activity is s ... | 2010 | 20074594 |
| biodegradation of naphthalene and anthracene by chemo-tactically active rhizobacteria of populus deltoides. | several naphthalene and anthracene degrading bacteria were isolated from rhizosphere of populus deltoides, which were growing in non-contaminated soil. among these, four isolates, i.e. kurthia sp., micrococcus varians, deinococcus radiodurans and bacillus circulans utilized chrysene, benzene, toluene and xylene, in addition to anthracene and naphthalene. kurthia sp and b. circulans showed positive chemotactic response for naphthalene and anthracene. the mean growth rate constant (k) of isolates ... | 2010 | 24031572 |
| bacterial degradation of fungicide captan. | the phthalimide fungicide captan has been widely used to control plant pathogenic fungi. a strain of bacillus circulans utilized the fungicide captan as sole source of carbon and energy. the organism degraded captan by a pathway involving its initial hydrolysis to yield cis-1,2,3,6-tetrahydrophthalimide, a compound without fungicidal activity. the formation of this compound was confirmed by hplc, ir, nmr, and mass spectral analysis. the results also revealed that cis-1,2,3,6-tetrahydrophthalimid ... | 2010 | 21121628 |
| enzymatic synthesis of lacto-n-difucohexaose i which binds to helicobacter pylori. | helicobacter pylori is known to bind with sugar chains possessing lewis b structure. we are trying to combine oligosaccharides containing lewis b sugar chain to water insoluble polysaccharide through some linker. lacto-n-difucohexaose i (lndfh i; fucalpha1-->2galbeta1-->3[fucalpha1-->4]glcnacbeta1-->3galbeta1-->4glc) fits for that purpose, since it consists of lewis b tetrasaccharide and lactose whose d-glucose residue can be utilized as a linker. we thus developed a method to synthesize this he ... | 2010 | 20816225 |
| thermostabilization of bacillus circulans xylanase: computational optimization of unstable residues based on thermal fluctuation analysis. | low thermostability often hampers the applications of xylanases in industrial processes operated at high temperature, such as degradation of biomass or pulp bleaching. thermostability of enzymes can be improved by the optimization of unstable residues via protein engineering. in this study, computational modeling instead of random mutagenesis was used to optimize unstable residues of bacillus circulans xylanase (bcx). the thermal fluctuations of unstable residues known as important to the therma ... | 2010 | 20959126 |
| deletion analysis of regions at the c-terminal part of cycloisomaltooligosaccharide glucanotransferase from bacillus circulans t-3040. | cycloisomaltooligosaccharide glucanotransferase (citase) belongs to glycoside hydrolase family 66. according to the sequence alignment of enzymes in the same family, we divided the structure of citase into five regions from the n terminus to the c terminus: an n-terminal conserved region (ser1-gly403), an insertion region (r1; tyr404-tyr492), two conserved regions (r2; glu493-ser596 and r3; gly597-met700), and a c-terminal variable region (r4; lys701-ser934). citase catalyzes the synthesis of cy ... | 2010 | 21193067 |