Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| bioremediation of endosulfan contaminated soil and water -- optimization of operating conditions in laboratory scale reactors. | a mixed bacterial culture consisted of staphylococcus sp., bacillus circulans-i and -ii has been enriched from contaminated soil collected from the vicinity of an endosulfan processing industry. the degradation of endosulfan by mixed bacterial culture was studied in aerobic and facultative anaerobic conditions via batch experiments with an initial endosulfan concentration of 50mg/l. after 3 weeks of incubation, mixed bacterial culture was able to degrade 71.58+/-0.2% and 75.88+/-0.2% of endosulf ... | 2006 | 16730891 |
| bacillus anthracis virulent plasmid px02 genes found in large plasmids of two other bacillus species. | in order to cause the disease anthrax, bacillus anthracis requires two plasmids, px01 and px02, which carry toxin and capsule genes, respectively, that are used as genetic targets in the laboratory detection of the bacterium. clinical, forensic, and environmental samples that test positive by pcr protocols established by the centers for disease control and prevention for b. anthracis are considered to be potentially b. anthracis until confirmed by culture and a secondary battery of tests. we rep ... | 2006 | 16825351 |
| cloning and expression of an alpha-1,3-glucanase gene from bacillus circulans ka-304: the enzyme participates in protoplast formation of schizophyllum commune. | a culture filtrate of bacillus circulans ka-304 grown on a cell-wall preparation of schizophyllum commune has an activity to form protoplasts from s. commune mycelia, and a combination of alpha-1,3-glucanase and chitinase i, which were isolated from the filtrate, brings about the protoplast-forming activity. the gene of alpha-1,3-glucanase was cloned from b. circulans ka-304. it consists of 3,879 nucleotides, which encodes 1,293 amino acids including a putative signal peptide (31 amino acid resi ... | 2006 | 16861810 |
| crystal structures of the plp- and pmp-bound forms of btrr, a dual functional aminotransferase involved in butirosin biosynthesis. | the aminotransferase (btrr), which is involved in the biosynthesis of butirosin, a 2-deoxystreptamine (2-dos)-containing aminoglycoside antibiotic produced by bacillus circulans, catalyses the pyridoxal phosphate (plp)-dependent transamination reaction both of 2-deoxy-scyllo-inosose to 2-deoxy-scyllo-inosamine and of amino-dideoxy-scyllo-inosose to 2-dos. the high-resolution crystal structures of the plp- and pmp-bound forms of btrr aminotransferase from b. circulans were solved at resolutions o ... | 2006 | 16894611 |
| structure of full-length bacterial chitinase containing two fibronectin type iii domains revealed by small angle x-ray scattering. | chitinase a1 (chia1) from bacillus circulans wl-12 consists of an n-terminal catalytic domain, two fibronectin type iii domains (fniiids), and a c-terminal chitin-binding domain. the full-length structure of chia1 was studied by small angle x-ray scattering. the obtained low-resolution structure showed that chia1 is an elongated molecule with a length of approximately 145 a composed of a large globular head and a rod-like tail. combination with known high-resolution structures of individual chia ... | 2006 | 16899221 |
| identification of catalytic amino acids of cyclodextran glucanotransferase from bacillus circulans t-3040. | in glycoside hydrolase family 66 (see http://afmb.cnrs-mrs.fr/cazy/), cyclodextran glucanotransferase (citase) is the only transglycosylation enzyme, all the other family 66 enzymes being dextranases. to analyze the catalytic amino acids of citase, we modified citase chemically from the t-3040 strain of bacillus circulans with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (edc). edc inactivated the enzyme by following pseudo-first order kinetics. in addition, the substrates of an isomaltooligos ... | 2006 | 16926507 |
| a novel glucanotransferase from a bacillus circulans strain that produces a cyclomaltopentaose cyclized by an alpha-1,6-linkage. | a novel glucanotransferase, involved in the synthesis of a cyclomaltopentaose cyclized by an alpha-1,6-linkage [icg5; cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}], from starch, was purified to homogeneity from the culture supernatant of bacillus circulans am7. the pi was estimated to be 7.5. the molecular mass of the enzyme was estimated to be 184 kda by gel filtration and 106 kda by sds-page. these results suggest that the ... | 2006 | 16926508 |
| bacteriocins reduce campylobacter colonization and alter gut morphology in turkey poults. | campylobacter is a leading cause of food-borne illness in the united states. recent evidence has demonstrated that bacteriocins produced by bacillus circulans and paenibacillus polymyxa reduce cecal campylobacter colonization in broiler chickens infected with campylobacter jejuni. as campylobacter coli is the most prevalent campylobacter isolate recovered in turkeys, the objectives of the present study were to evaluate the efficacy of these bacteriocins against c. coli colonization and their inf ... | 2006 | 16977842 |
| purification, characterization, and gene analysis of cellulase (cel8a) from lysobacter sp. ib-9374. | an enzyme that has both beta-1,4-glucanase and chitosanase activities was found in the culture medium of the soil bacterium lysobacter sp. ib-9374, a high lysyl endopeptidase-producing strain. the enzyme was purified to homogeneity from the culture filtrate using five purification steps and designated cel8a. the purified cel8a had a molecular mass of 41 kda, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. a ph optimum of 5.0 was found for the beta-1,4-glucanase activit ... | 2006 | 17031054 |
| role of glutamate 243 in the active site of 2-deoxy-scyllo-inosose synthase from bacillus circulans. | 2-deoxy-scyllo-inosose (doi) synthase is involved in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics and catalyzes the carbocyclic formation from d-glucose-6-phosphate (g-6-p) into doi. the reaction mechanism is proposed to be similar to that of dehydroquinate (dhq) synthase in the shikimate pathway, and includes oxidation of c-4, beta-elimination of phosphate, reduction of c-4, ring opening, and intramolecular aldol cyclization. to investigate the reaction mechanism ... | 2007 | 17035031 |
| cloning, sequencing, and expression of the genes encoding an isocyclomaltooligosaccharide glucanotransferase and an alpha-amylase from a bacillus circulans strain. | the gene for a novel glucanotransferase, isocyclomaltooligosaccharide glucanotransferase (igty), involved in the synthesis of a cyclomaltopentaose cyclized by an alpha-1,6-linkage [icg5; cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}] from starch, was cloned from the genome of b. circulans am7. the igty gene, designated igty, consisted of 2,985 bp encoding a signal peptide of 35 amino acids and a mature protein of 960 amino ac ... | 2006 | 17090949 |
| crystallization and preliminary x-ray analysis of the catalytic domain of chitinase d from bacillus circulans wl-12. | we report here on crystallization and preliminary x-ray analysis of the catalytic domain of chitinase d from bacillus circulans wl-12. the native crystals of this domain were found to belong to the orthorhombic space group p2(1)2(1)2(1). to elucidate the structure of the catalytic domain by the multiple isomorphous replacement method, 30 kinds of derivatized crystals were prepared by soaking the native crystals into a mother liquor containing salts of heavy metal atoms. difference patterson maps ... | 2006 | 17100652 |
| mechanism of stabilization of bacillus circulans xylanase upon the introduction of disulfide bonds. | the introduction of disulfide bonds has been used as a strategy to enhance the stability of bacillus circulans xylanase. the transition temperature of the s100c/n148c (ds1), v98c/a152c (ds2), and a1gc/g187,c188 (cxl) in comparison to the wild type was increased by 5.0, 4.1 and 3.8 degrees c, respectively. interestingly, a combination of two disulfide bonds of ds1 and cxl (cds1, circular disulfide 1) led to a 12 degrees c increase in the transition temperature. importantly, an increase in the mel ... | 2007 | 17141401 |
| reduction of starch granule size by expression of an engineered tandem starch-binding domain in potato plants. | granule size is an important parameter when using starch in industrial applications. an artificial tandem repeat of a family 20 starch-binding domain (sbd2) was engineered by two copies of the sbd derived from bacillus circulans cyclodextrin glycosyltransferase via the pro-thr-rich linker peptide from xyn10a from cellulomonas fimi. sbd2 and a single sbd were introduced into the amylose-free potato mutant, amf, using appropriate signal sequences. the accumulation of sbd2 into transgenic starch gr ... | 2004 | 17147616 |
| production of isocyclomaltopentaose from starch using isocyclomaltooligosaccharide glucanotransferase. | production of a novel cyclomaltopentaose cyclized by an alpha-1,6-linkage, [icg5; cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}], from starch was performed using isocyclomaltooligosaccharide glucanotransferase (igtase) derived from bacillus circulans am7. the optimal conditions for icg5-production from partially hydrolyzed starch were as follows: substrate concentration, 1.0% (w/v); ph, 5.5; temperature, 45 degrees c; reactio ... | 2006 | 17151467 |
| cyclodextrin production by cyclodextrin glycosyltransferase from bacillus circulans df 9r. | cyclodextrins (cd) are cyclic oligosaccharides with multiple applications in the food, pharmaceutical, cosmetic, agricultural and chemical industries. in this work, the conditions used to produce cd with cyclodextrin glycosyltransferase from bacillus circulans df 9r were optimized using experimental designs. the developed method allowed the partial purification and concentration of the enzyme from the cultural broth and, subsequently, the cd production, using the same cassava starch as enzyme ad ... | 2007 | 17174549 |
| elaboration of neosamine rings in the biosynthesis of neomycin and butirosin. | the proteins neo-11 and neo-18 encoded in the neomycin gene cluster (neo) of streptomyces fradiae ncimb 8233 have been characterized as glucosaminyl-6'-oxidase and 6'-oxoglucosaminyl:l-glutamate aminotransferase, respectively. the joint activity of neo-11 and neo-18 is responsible for the conversion of paromamine to neamine in the biosynthetic pathway of neomycin through a mechanism of fad-dependent dehydrogenation followed by a pyridoxal-5'-phosphate-mediated transamination. neo-18 is also show ... | 2007 | 17206729 |
| characterization of the enzyme btrd from bacillus circulans and revision of its functional assignment in the biosynthesis of butirosin. | 2007 | 17226887 | |
| mutanase from a paenibacillus isolate: nucleotide sequence of the gene and properties of recombinant enzymes. | a mutanase (alpha-1,3-glucanase)-producing microorganism was isolated from a soil sample and was identified as a relative of paenibacillus sp. the mutanase was purified to homogeneity from culture, and its molecular mass was around 57 kda. the gene for the mutanase was cloned by pcr using primers based on the n-terminal amino acid sequence of the purified enzyme. the determined nucleotide sequence of the gene consisted of 3651-bp open reading frame that encoded a predicted 1217-amino acid polype ... | 2007 | 17270351 |
| accurate, conformation-dependent predictions of solvent effects on protein ionization constants. | predicting how aqueous solvent modulates the conformational transitions and influences the pka values that regulate the biological functions of biomolecules remains an unsolved challenge. to address this problem, we developed fdpb_mf, a rotamer repacking method that exhaustively samples side chain conformational space and rigorously calculates multibody protein-solvent interactions. fdpb_mf predicts the effects on pka values of various solvent exposures, large ionic strength variations, strong e ... | 2007 | 17360348 |
| efficient production of 2-deoxy-scyllo-inosose from d-glucose by metabolically engineered recombinant escherichia coli. | 2-deoxy-scyllo-inosose (doi) is a six-membered carbocycle formed from d-glucose-6-phosphate catalyzed by 2-deoxy-scyllo-inosose synthase (dois), a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics. doi is valuable as a starting material for the benzene-free synthesis of catechol and other benzenoids. we constructed a series of metabolically engineered escherichia coli strains by introducing a dois gene (btrc) from bacillus circulans and disrupting genes fo ... | 2007 | 17368605 |
| a novel cyclic isomaltooligosaccharide (cycloisomaltodecaose, ci-10) produced by bacillus circulans t-3040 displays remarkable inclusion ability compared with cyclodextrins. | cyclodextrans (cis) are cyclic isomaltooligosaccharides and only ci-7, ci-8, and ci-9 were known. ci-7, ci-8, and ci-9, consisting of seven, eight, and nine glucoses, respectively, bound by alpha-(1-->6) linkages, are known to be produced by t-3040 strain of bacillus circulans. however, we have found, using 13c nmr and mass spectrometry, that this strain also produces ci-10, ci-11 and ci-12. these large cis are very soluble in water and inhibit the glucan synthesis of glucansucrases to the same ... | 2007 | 17433485 |
| biosynthesis of butirosin: transfer and deprotection of the unique amino acid side chain. | butirosin, an aminoglycoside antibiotic produced by bacillus circulans, bears the unique (s)-4-amino-2-hydroxybutyrate (ahba) side chain, which protects the antibiotic from several common resistance mechanisms. the ahba side chain is advantageously incorporated into clinically valuable antibiotics such as amikacin and arbekacin by synthetic methods. therefore, it is of significant interest to explore the biosynthetic origins of this useful moiety. we report here that the ahba side chain of butir ... | 2007 | 17462573 |
| unique o-ribosylation in the biosynthesis of butirosin. | using a comparative genetics approach, one or more of the btra, btrl, btrp, and btrv proteins encoded in the butirosin biosynthetic gene cluster (btr) from bacillus circulans sank72073 were identified as being responsible for an o-ribosylation process leading to the formation of ribostamycin, a key intermediate in this, and related antibiotic biosynthetic pathways. functional analysis of the recombinantly expressed proteins revealed that both btrl and btrp were responsible for the ribosylation o ... | 2007 | 17482823 |
| biological pretreatment with two bacterial strains for enzymatic hydrolysis of office paper. | the cellulose-hydrolyzing strains, sphingomonas paucimobilis mk1 and bacillus circulans mk2, were separated from soil and were grown together in a single culture plate. growth b. circulans mk2 in liquid culture required symbiosis with s. paucimobilis mk1. biological pretreatment with the combined strain suspension after the liquid culture improved enzymatic hydrolysis of office paper from municipal wastes. sugar recovery by s. paucimobilis mk1 (51%) was 1.4 times higher than that of the untreate ... | 2007 | 17487532 |
| recruitment of both uniform and differential binding energy in enzymatic catalysis: xylanases from families 10 and 11. | the contributions of enzyme-substrate hydrogen-binding interactions to catalysis by two different families of xylanases were evaluated through kinetic studies with two representative wild-type enzymes, cellulomonas fimi xylanase (cex) and bacillus circulans xylanase (bcx), on a series of monodeoxygenated and monodeoxyfluorinated p-nitrophenyl xylobioside substrates. effects of substitution in the distal (-2 subsite) sugar on kcat/km for cex were moderately large (up to 2.9 kcal mol-1), with no e ... | 2007 | 17503782 |
| decolorization of azo dyes and simulated dye bath wastewater using acclimatized microbial consortium--biostimulation and halo tolerance. | anaerobic acclimatization of activated sludge from a textile effluent treatment plant to high concentration of rb5 could effectively decolorize rb5 dye solution. the strains viz. pseudomonas aeruginosa and bacillus circulans and other unidentified laboratory isolates (nad1 and nad6) were predominantly present in the microbial consortium. the conditions for efficient decolorization, biostimulation to increase effectiveness of microbial consortium, its tolerance to high salt concentration and non- ... | 2008 | 17566726 |
| production of cyclodextrin glycosyltransferase by alkaliphilic bacillus circulans in submerged and solid-state cultivation. | cyclodextrin glycosyltransferase (cgtase; e.c. 2.4.1.19) is an industrially important enzyme, which is used to produce cyclodextrins (cds). in this research, we report the use of experimental factorial design to find the best conditions of ph and temperature for cgtase production by bacillus circulans var. alkalophilus. the optimized calculated values for the tested variables were, respectively, ph 9.7 and temperature 36 degrees c, with a cgtase activity of 615 u ml(-1). the cgtase production wa ... | 2007 | 17574546 |
| effect on epidermal cell of soybean protein-degraded products and structural determination of the root hair promoting peptide. | peptide(s) produced from degraded soybean protein by an alkaline protease from bacillus circulans ha12 (degraded soybean-meal products; dsp) increased the number of both the root hair cells (trichoblasts) and hairless cells (atrichoblasts) of brassica rapa by about 4.4 times and 1.9 times, respectively. to identify the root hair-promoting peptide(s) in dsp, the origin protein of the root hair-promoting peptide(s) was identified as kunitz trypsin inhibitor (kti). the root hair-promoting peptide i ... | 2007 | 17671783 |
| use of metabolic and molecular methods for the identification of a bacillus strain isolated from paper affected by foxing. | foxing of paper is a deterioration phenomenon occurring in the form of brown-yellowish spots, the abiotic and/or biotic causes of which are not yet completely understood. nevertheless, microbiological infection has been recognized that may contribute to paper damage and therefore it becomes important to know the taxonomic position and the degradative activity of the potential infectious biological agents which mostly are fungi, but also bacteria and yeasts. a cellulolytic bacterial strain isolat ... | 2008 | 17686620 |
| bacillus circulans wz-12 - a newly discovered aerobic dichloromethane-degrading methylotrophic bacterium. | a novel dichloromethane (dcm)-degrading bacterial strain named wz-12 (genbank accession no. ef100968) was isolated and identified as bacillus circulans based on standard morphological and physiological properties and nucleotide sequence analysis of enzymatically amplified 16s ribosomal deoxyribonucleic acid. dcm dehalogenase from b. circulans wz-12 was purified to 8.27-fold with a yield of 34.83%. the electrophoretically homogeneous-purified enzyme exhibited a specific activity of 118.82 u/mg. s ... | 2007 | 17687552 |
| terpenoids and flavonoids from laggera pterodonta. | to study the chemical constituents of aerial parts of laggera pterodonta (dc.) benth., the air-dried aerial parts of this plant were powered and extracted with boiling water and purified by silica gel column chromatography and recrystallized. eleven compounds were obtained from l. pterodonta. they were identified as to be 6-o-beta-d-glucopyranosyl-carvotanacetone (1), pterodontic acid (2), 1beta-hydroxy pterondontic acid (3), pterodontoside a (4), pterodondiol (5), pterodontriol b (6), 5-hydroxy ... | 2007 | 17703774 |
| competitive inhibition bacteria of bovine origin against salmonella serovars. | studies were conducted to isolate bacteria inhibitory to salmonella enterica serovar typhimurium definitive type (dt) 104 in vitro from cattle not carrying salmonella and to determine the inhibitory activity of the isolated bacteria through competitive growth in cattle feces artificially contaminated with salmonella typhimurium dt104 and s. enterica serovar newport. fecal samples (108) were obtained from dairy and beef cows. s. enterica serovars were isolated from 9.25% of the samples and includ ... | 2007 | 17803135 |
| a secondary xylan-binding site enhances the catalytic activity of a single-domain family 11 glycoside hydrolase. | bacillus circulans xylanase (bcx) is a single-domain family 11 glycoside hydrolase. using nmr-monitored titrations, we discovered that an inactive variant of this enzyme, e78q-bcx, bound xylooligosaccharides not only within its pronounced active site (as) cleft, but also at a distal surface region. chemical shift perturbation mapping and affinity electrophoresis, combined with mutational studies, identified the xylan-specific secondary binding site (sbs) as a shallow groove lined by asn, ser, an ... | 2007 | 17822716 |
| structure of 2-deoxy-scyllo-inosose synthase, a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics, in complex with a mechanism-based inhibitor and nad+. | a key enzyme in the biosynthesis of clinically important aminoglycoside antibiotics is 2-deoxy-scyllo-inosose synthase (dois), which catalyzes carbocycle formation from d-glucose-6-phosphate to 2-deoxy-scyllo-inosose through a multistep reaction. this reaction mechanism is similar to the catalysis by dehydroquinate synthase (dhqs) of the cyclization of 3-deoxy-d-arabino-heputulosonate-7-phosphate to dehydroquinate in the shikimate pathway, but significant dissimilarity between these enzymes is a ... | 2008 | 17879343 |
| modelling and optimization of fermentation factors for enhancement of alkaline protease production by isolated bacillus circulans using feed-forward neural network and genetic algorithm. | modelling and optimization of fermentation factors and evaluation for enhanced alkaline protease production by bacillus circulans. | 2008 | 17953681 |
| purification and immobilization of recombinant variants of brevundimonas diminuta glutaryl-7-aminocephalosporanic acid acylase expressed in escherichia coli cells. | modified chitin-binding domain (chbd) from bacillus circulans chitinase a1 with w42f mutation in chitin-binding site was genetically fused to different positions within alpha-subunit of glutaryl-7-aminocephalosporanic acid acylase (gla) gene. hybrid proteins were efficiently expressed in e. coli cells as soluble, enzymatically active and correctly processed holoenzymes. chbd-gla fusions were easily affinity purified on chitin column by changing the salt concentration of binding and elution buffe ... | 2008 | 17963935 |
| nucleotide and deduced amino acid sequences of mutanase-like genes from paenibacillus isolates: proposal of a new family of glycoside hydrolases. | three mutanase (alpha-1,3-glucanase)-producing microorganisms isolated from soil samples were identified as a relatives of paenibacillus. a mutanase was purified to homogeneity from cultures of each, and the molecular masses of the purified enzymes were approximately 132, 141, and 141kda, respectively. the corresponding three genes for mutanases were cloned by pcr using primers designed from each n-terminal amino acid sequence. another mutanase-like gene from one strain was also cloned by pcr us ... | 2008 | 17988780 |
| characterization and mechanistic study of a radical sam dehydrogenase in the biosynthesis of butirosin. | btrn encoded in the butirosin biosynthetic gene cluster possesses a cxxxcxxc motif conserved within the radical s-adenosyl methionine (sam) superfamily. its gene disruption in the butirosin producer bacillus circulans caused the interruption of the biosynthetic pathway between 2-deoxy-scyllo-inosamine (doia) and 2-deoxystreptamine (dos). further, in vitro assay of the overexpressed enzyme revealed that btrn catalyzed the oxidation of doia under the strictly anaerobic conditions along with consum ... | 2007 | 18001019 |
| characterization of nocardiopsis beta-1,3-glucanase with additional carbohydrate-binding domains. | beta-1,3-glucanase f (bglf) from alkaliphilic nocardiopsis sp. f96 is a single domain enzyme composed of only a catalytic domain. chimeric bglfs with some carbohydrate-binding domains were constructed and characterized. by connecting the c-terminal additional domain of beta-1,3-glucanase h from bacillus circulans iam1165 and the chitin-binding domain of chitinase j from alkaliphilic bacillus sp. j813, binding ability and hydrolyzing activity toward insoluble beta-1,3-glucans were both improved. | 2007 | 18029785 |
| shifting ph optimum of bacillus circulans xylanase based on molecular modeling. | although hydrolases are used in several industrial processes, its industrial applications have some limitations in specific cases since some industrial processes are carried out at ph value which is different from optimum ph of the enzyme. alkaline side optimum ph of hydrolases is always desirable, and it is proved difficult to achieve that by mutation only. hence, molecular modeling was applied to select the promising mutants. the changes in electrostatic potential, which was calculated using d ... | 2008 | 18077046 |
| circulin, an antibiotic from a member of the bacillus circulans group; bacteriological studies. | 1948 | 18101388 | |
| circulin, an antibiotic from an organism resembling bacillus circulans. | 1949 | 18116908 | |
| on the naming of two antibiotics from members of the bacillus circulans group; circulin and polypeptin. | 1949 | 18151943 | |
| determination of electrostatic interaction energies and protonation state populations in enzyme active sites. | the ph-dependence of the nmr chemical shift for titratable groups in proteins often deviate from a standard henderson-hasselbalch (hh) titration curve. a non-hh dependence of the chemical shift for a given residue can arise from a single-site, non-hh titrational event for that residue, or if the chemical shift of the group is influenced by additional titrational events occurring in other residues. we show that simultaneous fits of several non-hh nmr titration curves of interacting protein residu ... | 2008 | 18155242 |
| purification and characterization of chitinase a of streptomyces cyaneus sp-27: an enzyme participates in protoplast formation from schizophyllum commune mycelia. | a culture filtrate of bacillus circulans ka-304 grown on a cell-wall preparation of schizophyllum commune has an activity to form protoplasts from s. commune mycelia. alpha-1,3-glucanase and chitinase i, which were isolated from the filtrate, did not form the protoplast by itself while a mixture of them showed protoplast-forming activity. streptomyces cyaneus sp-27 was isolated based on the productivity of chitinase. the culture filtrate of s. cyaneus sp-27 did not form s. commune protoplasts, b ... | 2008 | 18175902 |
| antimicrobial potential of a lipopeptide biosurfactant derived from a marine bacillus circulans. | to isolate the biologically active fraction of the lipopeptide biosurfactant produced by a marine bacillus circulans and study its antimicrobial potentials. | 2008 | 18194244 |
| gene cloning, expression, and characterization of a novel beta-mannanase from bacillus circulans cgmcc 1416. | a dna fragment containing 2,079 base pairs from bacillus circulans cgmcc 1416 was cloned using degenerate pcr and inverse pcr. an open reading frame containing 981 bp was identified that encoded 326 amino acids residues, including a putative signal peptide of 31 residues. the deduced amino acid sequence showed the highest identity (68.1%) with endo-beta-1,4-d-mannanase from bacillus circulans strain k-1 of the glycoside hydrolase family 5 (gh5). the sequence encoding the mature protein was clone ... | 2008 | 18239434 |
| synthesis of a series of fructooligosaccharides with sucrose and cycloinulohexaose extending over ten degrees of polymerization using cycloinulooligosaccharide fructanotransferase from bacillus circulans okumz 31b. | prolonged incubation with sucrose as an acceptor and cycloinulohexaose as a donor at a high concentration of cycloinulooligosaccharide fructanotransferase afforded a series of fructooligosaccharides. their chain-length distribution extended over 10 degrees of polymerization when the donor concentration was increased to 120 mm. increasing the acceptor concentration proved effective in improving the yield of inulin-type oligosaccharides because hydrolysis was suppressed. | 2008 | 18391455 |
| comparative analysis of the diversity of aerobic spore-forming bacteria in raw milk from organic and conventional dairy farms. | bacterial contamination of raw milk can originate from different sources: air, milking equipment, feed, soil, faeces and grass. it is hypothesized that differences in feeding and housing strategies of cows may influence the microbial quality of milk. this assumption was investigated through comparison of the aerobic spore-forming flora in milk from organic and conventional dairy farms. laboratory pasteurized milk samples from five conventional and five organic dairy farms, sampled in late summer ... | 2008 | 18406093 |
| xylanase production by bacillus circulans d1 using maltose as carbon source. | bacillus circulans d1 is a good producer of extracellular thermostable xylanase. xylanase production in different carbon sources was evaluated and the enzyme synthesis was induced by various carbon sources. it was found that d-maltose is the best inducer of the enzyme synthesis (7.05 u/mg dry biomass at 48 h), while d-glucose and d-arabinose lead to the production of basal levels of xylanase. the crude enzyme solution is free of cellulases, even when the microorganism was cultivated in a medium ... | 2008 | 18421584 |
| structural simulation and protein engineering to convert an endo-chitosanase to an exo-chitosanase. | to obtain an enzyme for the production of chito-disaccharides (glcn(2)) by converting endo-chitosanase to exo-chitosanase, we chose an endo-chitosanase from bacillus circulans mh-k1 (csn) as the candidate for protein engineering. using molecular modeling, two peptides with five amino acids (pclgg) and six amino acids (srtckp) were designed and inserted after the positions of d(115) and t(222) of csn, respectively. the inserted fragments are expected to form loops that might protrude from opposit ... | 2008 | 18540010 |
| improved bioavailability and biodegradation of a model polyaromatic hydrocarbon by a biosurfactant producing bacterium of marine origin. | polyaromatic hydrocarbons (pahs) are organic pollutants mostly derived from the processing and combustion of fossil fuels and cause human health hazards. in the present study a marine biosurfactant producing strain of bacillus circulans was used to increase the bioavailability and consequent degradation of a model polyaromatic hydrocarbon, anthracene. although the organism could not utilize anthracene as the sole carbon source, it showed better growth and biosurfactant production in an anthracen ... | 2008 | 18565569 |
| studies on improving the immobilized bead reusability and alkaline protease production by isolated immobilized bacillus circulans (mtcc 6811) using overall evaluation criteria. | this study uses an overall evaluation criterion for improving the immobilized bead reusability and extracellular enzyme production by immobilized cells by assigning relative weightage to bead reusability, enzyme production, and cell leakage. initially, alkaline protease production by alginate-immobilized bacillus circulans (mtcc 6811) was analyzed using l18 orthogonal array (oa). the resultant optimized parameters were further fine-tuned with l9 oa experimentation. at l18-oa analysis, inoculum l ... | 2008 | 18568299 |
| the interaction of xylanases with commercial pulps. | when purified xylanases from trichoderma harzianum e58 or from a clone of bacillus circulans were incubated with various low-yield wood pulps, little of the original enzyme activity could be detected in the filtrate at the end of the reaction. partial bleaching of the pulps prior to enzymatic treatment generally resulted in an increased recovery of the xylanase activity. it appears that both nonspecific adsorption and soluble inhibitors may be responsible for the loss of much of the xylanase act ... | 1991 | 18597365 |
| hydrolysis of lactose in skim milk by immobilized beta-galactosidase (bacillus circulans). | a novel chemical reactor, consisting of beta-galactosidase from bacillus circulans immobilized onto a ribbed membrane made from polyvinylchloride and silica, was used to hydrolyze the lactose constituent of skim milk. multiresponse nonlinear regression methods were employed to determine the kinetic parameters of rate expressions based on a proposed enzymatic mechanism that includes the formation of oligosaccharides. high-performance liquid chromatography (hplc) methods were employed to monitor t ... | 1992 | 18600962 |
| cloning and expression of chitinase a gene from streptomyces cyaneus sp-27: the enzyme participates in protoplast formation of schizophyllum commune. | chitinase a of streptomyces cyaneus sp-27 or chitinase i of bacillus circulans ka-304 showed the protoplast-forming activity when combined with alpha-1,3-glucanase of b. circulans ka-304. the gene of chitinase a was cloned. it consisted of 903 nucleotides encoding 301 amino acid residues, including a putative signal peptide (35 amino acid residues). the deduced n-terminal moiety of chitinase a showed sequence homology with the chitin-binding domain of chitinase f from streptomyces coelicolor and ... | 2008 | 18603792 |
| mechanistic study on the reaction of a radical sam dehydrogenase btrn by electron paramagnetic resonance spectroscopy. | btrn is a radical sam ( s-adenosyl- l-methionine) enzyme that catalyzes the oxidation of 2-deoxy- scyllo-inosamine (doia) into 3-amino-2,3-dideoxy- scyllo-inosose (amino-doi) during the biosynthesis of 2-deoxystreptamine (dos) in the butirosin producer bacillus circulans. recently, we have shown that btrn catalyzes the transfer of a hydrogen atom at c-3 of doia to 5'-deoxyadenosine, and thus, the reaction was proposed to proceed through the hydrogen atom abstraction by the 5'-deoxyadenosyl radic ... | 2008 | 18672902 |
| chemoenzymatic acylation of aminoglycoside antibiotics. | the chemoenzymatic installation of the clinically valuable (s)-4-amino-2-hydroxybutyryl side chain onto a number of 2-deoxystreptamine-containing aminoglycosides is described using the purified bacillus circulans biosynthetic enzymes btrh and btrg in combination with a synthetic acyl-snac surrogate substrate. | 2008 | 18685777 |
| solid state bioreactor production of transglutaminase by amazonian bacillus circulans bl32 strain. | in this work, we investigated the production of transglutaminase (tgase) by an amazonian isolated strain of bacillus circulans by solid-state cultivation (ssc). several agro-industrial residues, such as untreated corn grits, milled brewers rice, industrial fibrous soy residue, soy hull, and malt bagasse, were used as substrates for microbial growth and enzyme production. growth on industrial fibrous soy residue, which is rich in protein and hemicellulose, produced the highest tgase activity (0.7 ... | 2008 | 18696133 |
| the cyclodextrin glycosyltransferase of paenibacillus pabuli us132 strain: molecular characterization and overproduction of the recombinant enzyme. | the gene encoding the cyclodextrin glycosyltransferase (cgtase) of paenibacillus pabuli us132, previously described as efficient antistaling agent and good candidate for cyclodextrins production, was cloned, sequenced, and expressed in escherichia coli. sequence analysis showed that the mature enzyme (684 amino acids) was preceded by a signal peptide of 34 residues. the enzyme exhibited the highest identity (94%) to the beta-cgtase of bacillus circulans no. 8. the production of the recombinant c ... | 2008 | 18704190 |
| environmental effects on transglutaminase production and cell sporulation in submerged cultivation of bacillus circulans. | in this research, the effects of ph, temperature, and oxygen on growth kinetics of a newly isolated strain of bacillus circulans from the amazon and their correlations with transglutaminase (tgase) production and cell sporulation were investigated. statistical experimental methods were used to optimize these parameters, while induction of sporulation was achieved by oxygen culture control. full factorial composite experimental design and response surface methodology were experimentally tested. t ... | 2009 | 18716920 |
| a novel beta-mannanase with high specific activity from bacillus circulans cgmcc1554: gene cloning, expression and enzymatic characterization. | a dna fragment of 2,042 bp containing a novel beta-mannanase gene, man5a, was identified from the genome of the mannan-degrading bacterium bacillus circulans cgmcc1554. the open reading frame of man5a comprised 978 bp encoding a protein of 326 amino acids with a predicted molecular weight of 32 kda. the amino acid sequence of the encoded mannanase, man5a, showed the highest identity (78.5%) to beta-mannanases belonging to glycosyl hydrolases family 5. the gene man5a was efficiently expressed in ... | 2009 | 18820840 |
| rapid quantification of a microbial surfactant by a simple turbidometric method. | quantification of the biosurfactants produced by a variety of microorganisms is a time taking and difficult task due to the lack of rapid, efficient and accurate methods. this work presents a simple turbidometric method for quantification of crude biosurfactants based on their property to become insoluble at low ph values. biosurfactants obtained from a bacillus sp. using different carbon substrates showed a good linear correlation (r(2)>0.99) between biosurfactant concentrations and turbidity i ... | 2009 | 18840480 |
| structure-function relationship in cyclodextrin glycosyltransferase from bacillus circulans df 9r. | cyclodextrin glycosyltransferases (cgtases e.c.2.4.1.19) catalyze cyclomaltooligosaccharides (cyclodextrins) production, an important industrial process. we herein report structural features of bacillus circulans df 9r cyclodextrin glycosyltransferase including its sequence and several aspects of enzyme structure-function relationship. protein ethoxyformylation, under our experimental conditions, indicated that only one out of the 13 enzyme histidines was modified leading to a drastic drop in cy ... | 2009 | 18922514 |
| effect of a probiotic bacterium bacillus circulans pb7 in the formulated diets: on growth, nutritional quality and immunity of catla catla (ham.). | bacillus circulans pb7, isolated from the intestine of catla catla, was evaluated for use as a probiotic supplement in the feeds for the fingerlings of catla catla. the effect of supplement on the growth performance, feed utilization efficiency, and immune response was evaluated. catla fingerlings (ave. wt. 6.48 +/- 0.43 g) were fed diets supplemented with 2 x 10(4) (feed c1), 2 x 10(5) (feed c2), and 2 x 10(6) (feed c3) b. circulans pb 7 cells per 100 g feed for 60 days at 5% of the body weight ... | 2009 | 18931930 |
| biological control of late leaf spot of peanut (arachis hypogaea) with chitinolytic bacteria. | abstract late leaf spot (lls), caused by phaeoisariopsis personata, is a foliar disease of groundnut or peanut (arachis hypogaea) with high economic and global importance. antifungal and chitinolytic bacillus circulans grs 243 and serratia marcescens gps 5, selected among a collection of 393 peanut-associated bacteria, were applied as a prophylactic foliar spray and tested for control of lls. chitin-supplemented application of b. circulans grs 243 and s. marcescens gps 5 resulted in improved bio ... | 2005 | 18943468 |
| [influence of different sources of carbon and nitrogen on the biosynthesis of proteolytic complexes by bacillus circulans 693, bacillus sp. 27 and yarrowia lipolytica 2061 strains]. | the influence of different factors on the biosynthesis of extracellular proteolytic complexes by strains-producers bacillus circulans 693, bacillus sp. 27 and yarrowia lipolytica 2061 at submerged cultivation has been investigated. it has been shown that ammonium hydrocarbonate and gelatin with glucose were optimum carbon and nitrogen sources for synthesis of proteolytic activity of b. circulans strain 693, gelatin with arabinose--for bacillus sp. 27, gelatin and glycine with sorbose--for y. lip ... | 2008 | 18959038 |
| enhancement of l-asparaginase production by isolated bacillus circulans (mtcc 8574) using response surface methodology. | l-asparaginase production was optimized using isolated bacillus circulans (mtcc 8574) under solid-state fermentation (ssf) using locally available agricultural waste materials. among different agricultural materials (red gram husk, bengal gram husk, coconut, and groundnut cake), red gram husk gave the maximum enzyme production. a wide range of ssf parameters were optimized for maximize the production of l-asparaginase. preliminary studies revealed that incubation temperature, moisture content, i ... | 2009 | 19052920 |
| bacillus circulans paracardiac infection in non-hodgkin lymphoma--a case report. | a case report is presented concerning bacillus circulans paracardiac infection in a 27 year old woman with non-hodgkin lymphoma. | 2008 | 19097386 |
| antimicrobial biosurfactants from marine bacillus circulans: extracellular synthesis and purification. | to purify the biosurfactant produced by a marine bacillus circulans strain and evaluate the improvement in surface and antimicrobial activities. | 2009 | 19187506 |
| role of chitin binding domain of chitinase a of streptomyces cyaneus sp-27 in protoplast formation from schizophyllum commune. | chitinase a (chia) of streptomyces cyaneus sp-27 forms protoplasts from schizophyllum commune mycelia when it is combined with alpha-1,3-glucanase of bacillus circulans ka-304. an n-terminal chitin-binding domain truncated mutant (catchia), which was expressed in escherichia coli rosetta-gami b (de 3), lost most of its colloidal chitin- and powder chitin-binding activity. the colloidal chitin-hydrolyzing, the powder chitin-hydrolyzing, and the protoplast-forming activities of catchia were lower ... | 2009 | 19270417 |
| identification and cloning of a gene encoding dichloromethane dehalogenase from a methylotrophic bacterium, bacillus circulans wz-12 cctcc m 207006. | the gene dehala encoding a novel dichloromethane dehalogenases (dehala), has been cloned from bacillus circulans wz-12 cctcc m 207006. the open reading frame of dehala, spanning 864 bp, encoded a 288-amino acid protein that showed 85.76% identity to the dichloromethane dehalogenases of hyphomicrobium sp. gj21 with several commonly conserved sequences. these sequences could not be found in putative dichloromethane (dcm) dehalogenases reported from other bacteria and fungi. dehala was expressed in ... | 2009 | 19277720 |
| effective enzymatic synthesis of lactosucrose and its analogues by beta-d-galactosidase from bacillus circulans. | in the present study, beta-d-galactosidase from bacillus circulans was proved to be a suitable biocatalyst for the production of lactosucrose (beta-d-galp-(1-->4)-alpha-d-glcp-(1-->2)-beta-d-fruf, i) and its analogues from lactose and sucrose. during the hydrolysis of lactose, the formation of four transfer products was followed by high performance liquid chromatography with refraction index detector. in addition, the transfer products were isolated from the reaction mixture and identified to be ... | 2009 | 19326862 |
| characterization of temperature dependent and substrate-binding cleft movements in bacillus circulans family 11 xylanase: a molecular dynamics investigation. | xylanases (ec 3.2.1.8) hydrolyze xylan, one of the most abundant plant polysaccharides found in nature, and have many potential applications in biotechnology. | 2009 | 19409448 |
| additional carbohydrate-binding modules enhance the insoluble substrate-hydrolytic activity of beta-1,3-glucanase from alkaliphilic nocardiopsis sp. f96. | beta-1,3-glucanase (bglf) from nocardiopsis sp. f96 is composed of only a catalytic domain. to improve the enzymatic properties of bglf, we attempted to construct chimeric enzymes consisting of bglf and some carbohydrate-binding modules, such as the c-terminal additional domain (cad) and the n-terminal additional domain (nad) of beta-1,3-glucanase h from bacillus circulans iam1165 and the chitin-binding domain (chbd) of chitinase from alkaliphilic bacillus sp. j813. cad-fused bglf (bglf-cad), na ... | 2009 | 19420727 |
| [biosynthesis of secreted ribonucleases by bacillus intermedius and bacillus circulans during nitrogen starvation]. | 2009 | 19449735 | |
| a robust method for the joint estimation of yield coefficients and kinetic parameters in bioprocess models. | bioprocess model structures that require nonlinear parameter estimation, thus initialization values, are often subject to poor identification performances because of the uncertainty on those initialization values. under some conditions on the model structure, it is possible to partially circumvent this problem by an appropriate decoupling of the linear part of the model from the nonlinear part of it. this article provides a procedure to be followed when these structural conditions are not satisf ... | 2009 | 19455623 |
| evaluation of antineoplastic activity of extracellular asparaginase produced by isolated bacillus circulans. | l-asparaginase is an important component in the treatment of acute lymphoblastic leukemia in children. its antineoplastic activity toward malignant cells is due to their characteristic nature in slow synthesis of l-asparagine (asn), which causes starvation for this amino acid, while normal cells are protected from asn starvation due to their ability to produce this amino acid. the relative selectivity with regard to the metabolism of malignant cells forces to look for novel asparaginase with lit ... | 2010 | 19543838 |
| survey of bacterial populations present in us-produced linerboard with high recycle content. | to survey paperboard products from 17 us mills for bacterial populations and for bacteria potentially harmful to human health. | 2010 | 19645765 |
| in vitro antimicrobial effect of satureja wiedemanniana against bacillus species isolated from raw meat samples. | in this study a total of 30 raw meat samples obtained from ankara, turkey were screened for the presence of bacillus species. among the meat samples analyzed, the predominant species isolated was bacillus circulans; other bacillus species were identified as bacillus firmus, bacillus lentus, bacillus megaterium, bacillus licheniformis, bacillus mycoides, bacillus sphaericus, and bacillus cereus. minced meat samples were more contaminated with bacillus species than sliced beef sample. from these s ... | 2009 | 19735196 |
| [gene cloning and overexpression of dichloromethane dehalogenase from bacillus circulans wz-12]. | the dcmr gene encoding dichloromethane dehalogenase was amplified by pcr from bacillus circulans wz-12 and cloned to expression vector pet28b(+), yielding recombinant plasmid pet28b(+)-dcmr. then plasmid pet28b(+)-dcmr was introduced into escherichia. coli bl21(de3). expression was induced by iptg,and the enzyme activity reached 25.78 u/ml, the specific enzyme activity reached 88.86 u/mg protein.the periplasmic and cytoplasmic enzyme activity reached 2.92 u/ml and 22.86 u/ml respectively.all res ... | 2009 | 19799320 |
| static magnetic field exposure fails to affect the viability of different bacteria strains. | the viability of the microbes saccharomyces cerevisiae, bacillus circulans, escherichia coli, micrococcus luteus, pseudomonas fluorescens, salmonella enteritidis, serratia marcescens, and staphylococcus aureus was tested under static magnetic field exposure up to 24 h in either a homogeneous (159.2 +/- 13.4 mt) or three types of inhomogeneous static magnetic fields: (i) peak-to-peak magnetic flux density 476.7 +/- 0.1 mt with a lateral magnetic flux density gradient of 47.7 t/m, (ii) 12.0 +/- 0. ... | 2010 | 19821495 |
| analysis of the involvement of chitin-binding domain of chicw in antifungal activity, and engineering a novel chimeric chitinase with high enzyme and antifungal activities. | an antifungal chitinase, chicw, produced by bacillus cereus 28-9 is effective against conidial germination of botrytis elliptica, the causal agent of lily leaf blight. chicw as a modular enzyme consists of a signal peptide, a catalytic domain, a fibronectin type-iii-like domain, and a chitin-binding domain. when two c-terminal domains of chicw were truncated, chicwdeltafc (lacking the chitin-binding domain and fibronectin type iii-like domain) lost its antifungal activity. since chicwdeltaac (la ... | 2009 | 19884776 |
| facile pretreatment of bacillus circulans beta-galactosidase increases the yield of galactosyl oligosaccharides in milk and lactose reaction systems. | the commercially available preparation of beta-galactosidase from bacillus circulans , known as biolacta fn5, has been extensively used in the production of prebiotic galactooligosaccharides (gos). this study focuses on characterizing the production of gos in two reaction systems: 10% lactose (w/v) in buffer and skim milk. analysis of the temperature dependence of the gos yield along with the relative rates of gos synthesis and degradation leads to the finding that gos degradation activity was s ... | 2009 | 19921828 |
| identification and application of aflp-derived genetic markers for quantitative pcr-based tracking of bacillus and paenibacillus spp. released in soil. | in this study, we show that noncoding sequences from amplified fragment length polymorphisms (aflps) can provide robust and sensitive genetic markers suitable for pcr-based discrimination of closely related strains of bacillus and paenibacillus, and quantitative pcr (qpcr)-based tracking of the strains in complex natural systems like soil. quantitative pcr was accurate in the approximately 1 x 10(9) to approximately 1 x 10(4) colony forming units (cfu)/g soil range. the detection limit was impro ... | 2009 | 19935889 |
| thermostabilization of bacillus circulans xylanase via computational design of a flexible surface cavity. | despite recent advances in our understanding of the importance of protein-surface properties for protein thermostability, to date many rational designs have been focused instead on protein-core characteristics such as core packing and cavity filling. rational strategies to design protein surfaces to improve protein thermostability have not yet been well investigated. here, an efficient rational design of a surface cavity for improving protein thermostability without reducing enzyme activity is s ... | 2010 | 20074594 |
| enzymatic thioxyloside synthesis: characterization of thioglycoligase variants identified from a site-saturation mutagenesis library of bacillus circulans xylanase. | thioglycoligases are engineered enzymes for the synthesis of thioglycosides that are derived from retaining glycosidases by replacing the acid/base catalyst. the optimal choice of substitution for the acid/base mutant is currently unknown, so to investigate this question a complete acid/base library of the model glycosidase bacillus circulans xylanase (bcx) was generated by using site-saturation mutagenesis. a novel screening approach combining active site titration with semiquantitative product ... | 2010 | 20112321 |
| circular permutation of bacillus circulans xylanase: a kinetic and structural study. | the 20 kda bacillus circulans bcx is a well-studied endoxylanase with a beta-jellyroll fold that places its n- and c-termini in salt bridge contact. initial experiments verified that bcx could be circularly permuted by pcr methods to introduce new termini in loop regions while linking its native termini directly or via one or two glycines. subsequently, a library of circular permutants, generated by random dnase cleavage of the circularized bcx gene, was screened for xylanase activity on xylan i ... | 2010 | 20163191 |
| improvement of bacillus circulans beta-amylase activity attained using the ancestral mutation method. | thermostabilization of enzymes is one of the greatest challenges of protein engineering. the ancestral mutation method, which introduces ancestral residues into a target enzyme, has previously been developed and used to improve the thermostabilities of thermophilic enzymes. herein, we report a study that used the ancestral mutation method to improve the thermostability of bacillus circulans beta-amylase, a mesophilic enzyme. a bacterial, common-ancestral beta-amylase sequence was inferred using ... | 2010 | 20406825 |
| the effect of ph and chemical preservatives on the growth of bacterial isolates from some nigerian packaged fruit juices. | bacterial pathogens were isolated from some nigerian packaged fruit juices. the isolates were characterized and identified as bacillus licheniformis, aeromonas hydrophila, bacillus circulans, proteus morganii, pseudomonas cepacia, bacillus alvei and pseudomonas chlororaphis. the antibiotic susceptibility profile of the seven isolates was determined and it was discovered that 65% of the microorganisms isolated were resistance to the antibiotic used while 35% were sensitive. the effect of ph, benz ... | 2010 | 20415148 |
| roles of a 20 kda protein associated with a carbocycle-forming enzyme involved in aminoglycoside biosynthesis in primary and secondary metabolism. | 2-deoxy-scyllo-inosose (doi) synthase participates in the biosynthesis of 2-deoxystreptamine (dos)-containing aminoglycoside antibiotics. the enzyme is expected to be of industrial use, because it converts a sustainable resource (glucose 6-phosphate) into carbocycle (doi), which easily aromatizes to yield catechol. in the present study, we clarified the physiological role of a non-catalytic 20 kda protein, btrc2, associated with doi synthase from the butirosin-producer bacillus circulans. based ... | 2010 | 20530911 |
| the effects of organic solvents on the efficiency and regioselectivity of n-acetyl-lactosamine synthesis, using the β-galactosidase from bacillus circulans in hydro-organic media. | the enzymatic synthesis of n-acetyl-lactosamine (lacnac) by the transgalactosylation of n-acetyl-d-glucosamine (glcnac), catalyzed by the β-galactosidase from bacillus circulans (bcβgal), was studied in hydro-organic media, starting from o-nitrophenyl-β-d-galactopyranoside (onpg) as a galactosyl donor. thermal stability and synthesis activity of bcβgal were shown to depend on the organic solvent polarity, characterized by its log p value. bcβgal was thus most stable in 10% (v/v) t-buoh, an organ ... | 2010 | 20568279 |
| effect of size, quaternary structure and translational error on the static and dynamic heterogeneity of beta-galactosidase and measurement of electrophoretic dynamic heterogeneity. | single enzyme molecule assays were performed using capillary electrophoresis-based protocols on beta-galactosidase from lactobacillus delbrueckii, lactobacillus reuteri, lactobacillus helveticus and bacillus circulans. the enzyme was found to show static heterogeneity with respect to catalytic rate and the variance in rate increased with protein size. this is consistent with the proposal that random errors in translation may be an important underlying component of enzyme heterogeneity. additiona ... | 2010 | 20607374 |
| enzymatic synthesis and characterization of hydroquinone galactoside using kluyveromyces lactis lactase. | hydroquinone galactoside (hq-gal) as a potential skin whitening agent was synthesized by the reaction of lactase (beta-galactosidase) from kluyveromyces lactis, aspergillus oryzae, bacillus circulans, and thermus sp. with lactose as a donor and hq as an acceptor. among these lactases, the acceptor reaction involving hq and lactose with k. lactis lactase showed a higher conversion ratio to hq-gal (60.27%). hq-gal was purified using butanol partitioning and silica gel column chromatography. the st ... | 2010 | 20687552 |
| measuring "unmeasurable" folding kinetics of proteins by single-molecule force spectroscopy. | folding and unfolding are fundamental biological processes in cell and are important for the biological functions of proteins. characterizing the folding and unfolding kinetics of proteins is important for understanding the energetic landscape leading to the active native conformations of these molecules. however, the thermal or chemical-induced unfolding of many proteins is irreversible in vitro, precluding characterization of the folding kinetics of such proteins, just as it is impossible to " ... | 2010 | 20691701 |
| enzymatic synthesis of lacto-n-difucohexaose i which binds to helicobacter pylori. | helicobacter pylori is known to bind with sugar chains possessing lewis b structure. we are trying to combine oligosaccharides containing lewis b sugar chain to water insoluble polysaccharide through some linker. lacto-n-difucohexaose i (lndfh i; fucalpha1-->2galbeta1-->3[fucalpha1-->4]glcnacbeta1-->3galbeta1-->4glc) fits for that purpose, since it consists of lewis b tetrasaccharide and lactose whose d-glucose residue can be utilized as a linker. we thus developed a method to synthesize this he ... | 2010 | 20816225 |
| isolation and characterization of bacteria from the gut of bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion. | bombyx mori l. (lepidoptera: bombycidae) have been domesticated and widely used for silk production. it feeds on mulberry leaves. mulberry leaves are mainly composed of pectin, xylan, cellulose and starch. some of the digestive enzymes that degrade these carbohydrates might be produced by gut bacteria. eleven isolates were obtained from the digestive tract of b. mori, including the gram positive bacillus circulans and gram negative proteus vulgaris, klebsiella pneumoniae, escherichia coli, citro ... | 2010 | 20874394 |
| thermostabilization of bacillus circulans xylanase: computational optimization of unstable residues based on thermal fluctuation analysis. | low thermostability often hampers the applications of xylanases in industrial processes operated at high temperature, such as degradation of biomass or pulp bleaching. thermostability of enzymes can be improved by the optimization of unstable residues via protein engineering. in this study, computational modeling instead of random mutagenesis was used to optimize unstable residues of bacillus circulans xylanase (bcx). the thermal fluctuations of unstable residues known as important to the therma ... | 2010 | 20959126 |
| bacterial degradation of fungicide captan. | the phthalimide fungicide captan has been widely used to control plant pathogenic fungi. a strain of bacillus circulans utilized the fungicide captan as sole source of carbon and energy. the organism degraded captan by a pathway involving its initial hydrolysis to yield cis-1,2,3,6-tetrahydrophthalimide, a compound without fungicidal activity. the formation of this compound was confirmed by hplc, ir, nmr, and mass spectral analysis. the results also revealed that cis-1,2,3,6-tetrahydrophthalimid ... | 2010 | 21121628 |