Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| improved bioavailability and biodegradation of a model polyaromatic hydrocarbon by a biosurfactant producing bacterium of marine origin. | polyaromatic hydrocarbons (pahs) are organic pollutants mostly derived from the processing and combustion of fossil fuels and cause human health hazards. in the present study a marine biosurfactant producing strain of bacillus circulans was used to increase the bioavailability and consequent degradation of a model polyaromatic hydrocarbon, anthracene. although the organism could not utilize anthracene as the sole carbon source, it showed better growth and biosurfactant production in an anthracen ... | 2008 | 18565569 |
| structural simulation and protein engineering to convert an endo-chitosanase to an exo-chitosanase. | to obtain an enzyme for the production of chito-disaccharides (glcn(2)) by converting endo-chitosanase to exo-chitosanase, we chose an endo-chitosanase from bacillus circulans mh-k1 (csn) as the candidate for protein engineering. using molecular modeling, two peptides with five amino acids (pclgg) and six amino acids (srtckp) were designed and inserted after the positions of d(115) and t(222) of csn, respectively. the inserted fragments are expected to form loops that might protrude from opposit ... | 2008 | 18540010 |
| xylanase production by bacillus circulans d1 using maltose as carbon source. | bacillus circulans d1 is a good producer of extracellular thermostable xylanase. xylanase production in different carbon sources was evaluated and the enzyme synthesis was induced by various carbon sources. it was found that d-maltose is the best inducer of the enzyme synthesis (7.05 u/mg dry biomass at 48 h), while d-glucose and d-arabinose lead to the production of basal levels of xylanase. the crude enzyme solution is free of cellulases, even when the microorganism was cultivated in a medium ... | 2008 | 18421584 |
| comparative analysis of the diversity of aerobic spore-forming bacteria in raw milk from organic and conventional dairy farms. | bacterial contamination of raw milk can originate from different sources: air, milking equipment, feed, soil, faeces and grass. it is hypothesized that differences in feeding and housing strategies of cows may influence the microbial quality of milk. this assumption was investigated through comparison of the aerobic spore-forming flora in milk from organic and conventional dairy farms. laboratory pasteurized milk samples from five conventional and five organic dairy farms, sampled in late summer ... | 2008 | 18406093 |
| synthesis of a series of fructooligosaccharides with sucrose and cycloinulohexaose extending over ten degrees of polymerization using cycloinulooligosaccharide fructanotransferase from bacillus circulans okumz 31b. | prolonged incubation with sucrose as an acceptor and cycloinulohexaose as a donor at a high concentration of cycloinulooligosaccharide fructanotransferase afforded a series of fructooligosaccharides. their chain-length distribution extended over 10 degrees of polymerization when the donor concentration was increased to 120 mm. increasing the acceptor concentration proved effective in improving the yield of inulin-type oligosaccharides because hydrolysis was suppressed. | 2008 | 18391455 |
| gene cloning, expression, and characterization of a novel beta-mannanase from bacillus circulans cgmcc 1416. | a dna fragment containing 2,079 base pairs from bacillus circulans cgmcc 1416 was cloned using degenerate pcr and inverse pcr. an open reading frame containing 981 bp was identified that encoded 326 amino acids residues, including a putative signal peptide of 31 residues. the deduced amino acid sequence showed the highest identity (68.1%) with endo-beta-1,4-d-mannanase from bacillus circulans strain k-1 of the glycoside hydrolase family 5 (gh5). the sequence encoding the mature protein was clone ... | 2008 | 18239434 |
| antimicrobial potential of a lipopeptide biosurfactant derived from a marine bacillus circulans. | to isolate the biologically active fraction of the lipopeptide biosurfactant produced by a marine bacillus circulans and study its antimicrobial potentials. | 2008 | 18194244 |
| purification and characterization of chitinase a of streptomyces cyaneus sp-27: an enzyme participates in protoplast formation from schizophyllum commune mycelia. | a culture filtrate of bacillus circulans ka-304 grown on a cell-wall preparation of schizophyllum commune has an activity to form protoplasts from s. commune mycelia. alpha-1,3-glucanase and chitinase i, which were isolated from the filtrate, did not form the protoplast by itself while a mixture of them showed protoplast-forming activity. streptomyces cyaneus sp-27 was isolated based on the productivity of chitinase. the culture filtrate of s. cyaneus sp-27 did not form s. commune protoplasts, b ... | 2008 | 18175902 |
| determination of electrostatic interaction energies and protonation state populations in enzyme active sites. | the ph-dependence of the nmr chemical shift for titratable groups in proteins often deviate from a standard henderson-hasselbalch (hh) titration curve. a non-hh dependence of the chemical shift for a given residue can arise from a single-site, non-hh titrational event for that residue, or if the chemical shift of the group is influenced by additional titrational events occurring in other residues. we show that simultaneous fits of several non-hh nmr titration curves of interacting protein residu ... | 2008 | 18155242 |
| on the naming of two antibiotics from members of the bacillus circulans group; circulin and polypeptin. | 1949 | 18151943 | |
| circulin, an antibiotic from an organism resembling bacillus circulans. | 1949 | 18116908 | |
| circulin, an antibiotic from a member of the bacillus circulans group; bacteriological studies. | 1948 | 18101388 | |
| shifting ph optimum of bacillus circulans xylanase based on molecular modeling. | although hydrolases are used in several industrial processes, its industrial applications have some limitations in specific cases since some industrial processes are carried out at ph value which is different from optimum ph of the enzyme. alkaline side optimum ph of hydrolases is always desirable, and it is proved difficult to achieve that by mutation only. hence, molecular modeling was applied to select the promising mutants. the changes in electrostatic potential, which was calculated using d ... | 2008 | 18077046 |
| characterization of nocardiopsis beta-1,3-glucanase with additional carbohydrate-binding domains. | beta-1,3-glucanase f (bglf) from alkaliphilic nocardiopsis sp. f96 is a single domain enzyme composed of only a catalytic domain. chimeric bglfs with some carbohydrate-binding domains were constructed and characterized. by connecting the c-terminal additional domain of beta-1,3-glucanase h from bacillus circulans iam1165 and the chitin-binding domain of chitinase j from alkaliphilic bacillus sp. j813, binding ability and hydrolyzing activity toward insoluble beta-1,3-glucans were both improved. | 2007 | 18029785 |
| characterization and mechanistic study of a radical sam dehydrogenase in the biosynthesis of butirosin. | btrn encoded in the butirosin biosynthetic gene cluster possesses a cxxxcxxc motif conserved within the radical s-adenosyl methionine (sam) superfamily. its gene disruption in the butirosin producer bacillus circulans caused the interruption of the biosynthetic pathway between 2-deoxy-scyllo-inosamine (doia) and 2-deoxystreptamine (dos). further, in vitro assay of the overexpressed enzyme revealed that btrn catalyzed the oxidation of doia under the strictly anaerobic conditions along with consum ... | 2007 | 18001019 |
| nucleotide and deduced amino acid sequences of mutanase-like genes from paenibacillus isolates: proposal of a new family of glycoside hydrolases. | three mutanase (alpha-1,3-glucanase)-producing microorganisms isolated from soil samples were identified as a relatives of paenibacillus. a mutanase was purified to homogeneity from cultures of each, and the molecular masses of the purified enzymes were approximately 132, 141, and 141kda, respectively. the corresponding three genes for mutanases were cloned by pcr using primers designed from each n-terminal amino acid sequence. another mutanase-like gene from one strain was also cloned by pcr us ... | 2008 | 17988780 |
| purification and immobilization of recombinant variants of brevundimonas diminuta glutaryl-7-aminocephalosporanic acid acylase expressed in escherichia coli cells. | modified chitin-binding domain (chbd) from bacillus circulans chitinase a1 with w42f mutation in chitin-binding site was genetically fused to different positions within alpha-subunit of glutaryl-7-aminocephalosporanic acid acylase (gla) gene. hybrid proteins were efficiently expressed in e. coli cells as soluble, enzymatically active and correctly processed holoenzymes. chbd-gla fusions were easily affinity purified on chitin column by changing the salt concentration of binding and elution buffe ... | 2008 | 17963935 |
| modelling and optimization of fermentation factors for enhancement of alkaline protease production by isolated bacillus circulans using feed-forward neural network and genetic algorithm. | modelling and optimization of fermentation factors and evaluation for enhanced alkaline protease production by bacillus circulans. | 2008 | 17953681 |
| structure of 2-deoxy-scyllo-inosose synthase, a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics, in complex with a mechanism-based inhibitor and nad+. | a key enzyme in the biosynthesis of clinically important aminoglycoside antibiotics is 2-deoxy-scyllo-inosose synthase (dois), which catalyzes carbocycle formation from d-glucose-6-phosphate to 2-deoxy-scyllo-inosose through a multistep reaction. this reaction mechanism is similar to the catalysis by dehydroquinate synthase (dhqs) of the cyclization of 3-deoxy-d-arabino-heputulosonate-7-phosphate to dehydroquinate in the shikimate pathway, but significant dissimilarity between these enzymes is a ... | 2008 | 17879343 |
| a secondary xylan-binding site enhances the catalytic activity of a single-domain family 11 glycoside hydrolase. | bacillus circulans xylanase (bcx) is a single-domain family 11 glycoside hydrolase. using nmr-monitored titrations, we discovered that an inactive variant of this enzyme, e78q-bcx, bound xylooligosaccharides not only within its pronounced active site (as) cleft, but also at a distal surface region. chemical shift perturbation mapping and affinity electrophoresis, combined with mutational studies, identified the xylan-specific secondary binding site (sbs) as a shallow groove lined by asn, ser, an ... | 2007 | 17822716 |
| competitive inhibition bacteria of bovine origin against salmonella serovars. | studies were conducted to isolate bacteria inhibitory to salmonella enterica serovar typhimurium definitive type (dt) 104 in vitro from cattle not carrying salmonella and to determine the inhibitory activity of the isolated bacteria through competitive growth in cattle feces artificially contaminated with salmonella typhimurium dt104 and s. enterica serovar newport. fecal samples (108) were obtained from dairy and beef cows. s. enterica serovars were isolated from 9.25% of the samples and includ ... | 2007 | 17803135 |
| terpenoids and flavonoids from laggera pterodonta. | to study the chemical constituents of aerial parts of laggera pterodonta (dc.) benth., the air-dried aerial parts of this plant were powered and extracted with boiling water and purified by silica gel column chromatography and recrystallized. eleven compounds were obtained from l. pterodonta. they were identified as to be 6-o-beta-d-glucopyranosyl-carvotanacetone (1), pterodontic acid (2), 1beta-hydroxy pterondontic acid (3), pterodontoside a (4), pterodondiol (5), pterodontriol b (6), 5-hydroxy ... | 2007 | 17703774 |
| bacillus circulans wz-12 - a newly discovered aerobic dichloromethane-degrading methylotrophic bacterium. | a novel dichloromethane (dcm)-degrading bacterial strain named wz-12 (genbank accession no. ef100968) was isolated and identified as bacillus circulans based on standard morphological and physiological properties and nucleotide sequence analysis of enzymatically amplified 16s ribosomal deoxyribonucleic acid. dcm dehalogenase from b. circulans wz-12 was purified to 8.27-fold with a yield of 34.83%. the electrophoretically homogeneous-purified enzyme exhibited a specific activity of 118.82 u/mg. s ... | 2007 | 17687552 |
| use of metabolic and molecular methods for the identification of a bacillus strain isolated from paper affected by foxing. | foxing of paper is a deterioration phenomenon occurring in the form of brown-yellowish spots, the abiotic and/or biotic causes of which are not yet completely understood. nevertheless, microbiological infection has been recognized that may contribute to paper damage and therefore it becomes important to know the taxonomic position and the degradative activity of the potential infectious biological agents which mostly are fungi, but also bacteria and yeasts. a cellulolytic bacterial strain isolat ... | 2008 | 17686620 |
| effect on epidermal cell of soybean protein-degraded products and structural determination of the root hair promoting peptide. | peptide(s) produced from degraded soybean protein by an alkaline protease from bacillus circulans ha12 (degraded soybean-meal products; dsp) increased the number of both the root hair cells (trichoblasts) and hairless cells (atrichoblasts) of brassica rapa by about 4.4 times and 1.9 times, respectively. to identify the root hair-promoting peptide(s) in dsp, the origin protein of the root hair-promoting peptide(s) was identified as kunitz trypsin inhibitor (kti). the root hair-promoting peptide i ... | 2007 | 17671783 |
| production of cyclodextrin glycosyltransferase by alkaliphilic bacillus circulans in submerged and solid-state cultivation. | cyclodextrin glycosyltransferase (cgtase; e.c. 2.4.1.19) is an industrially important enzyme, which is used to produce cyclodextrins (cds). in this research, we report the use of experimental factorial design to find the best conditions of ph and temperature for cgtase production by bacillus circulans var. alkalophilus. the optimized calculated values for the tested variables were, respectively, ph 9.7 and temperature 36 degrees c, with a cgtase activity of 615 u ml(-1). the cgtase production wa ... | 2007 | 17574546 |
| decolorization of azo dyes and simulated dye bath wastewater using acclimatized microbial consortium--biostimulation and halo tolerance. | anaerobic acclimatization of activated sludge from a textile effluent treatment plant to high concentration of rb5 could effectively decolorize rb5 dye solution. the strains viz. pseudomonas aeruginosa and bacillus circulans and other unidentified laboratory isolates (nad1 and nad6) were predominantly present in the microbial consortium. the conditions for efficient decolorization, biostimulation to increase effectiveness of microbial consortium, its tolerance to high salt concentration and non- ... | 2008 | 17566726 |
| recruitment of both uniform and differential binding energy in enzymatic catalysis: xylanases from families 10 and 11. | the contributions of enzyme-substrate hydrogen-binding interactions to catalysis by two different families of xylanases were evaluated through kinetic studies with two representative wild-type enzymes, cellulomonas fimi xylanase (cex) and bacillus circulans xylanase (bcx), on a series of monodeoxygenated and monodeoxyfluorinated p-nitrophenyl xylobioside substrates. effects of substitution in the distal (-2 subsite) sugar on kcat/km for cex were moderately large (up to 2.9 kcal mol-1), with no e ... | 2007 | 17503782 |
| biological pretreatment with two bacterial strains for enzymatic hydrolysis of office paper. | the cellulose-hydrolyzing strains, sphingomonas paucimobilis mk1 and bacillus circulans mk2, were separated from soil and were grown together in a single culture plate. growth b. circulans mk2 in liquid culture required symbiosis with s. paucimobilis mk1. biological pretreatment with the combined strain suspension after the liquid culture improved enzymatic hydrolysis of office paper from municipal wastes. sugar recovery by s. paucimobilis mk1 (51%) was 1.4 times higher than that of the untreate ... | 2007 | 17487532 |
| unique o-ribosylation in the biosynthesis of butirosin. | using a comparative genetics approach, one or more of the btra, btrl, btrp, and btrv proteins encoded in the butirosin biosynthetic gene cluster (btr) from bacillus circulans sank72073 were identified as being responsible for an o-ribosylation process leading to the formation of ribostamycin, a key intermediate in this, and related antibiotic biosynthetic pathways. functional analysis of the recombinantly expressed proteins revealed that both btrl and btrp were responsible for the ribosylation o ... | 2007 | 17482823 |
| biosynthesis of butirosin: transfer and deprotection of the unique amino acid side chain. | butirosin, an aminoglycoside antibiotic produced by bacillus circulans, bears the unique (s)-4-amino-2-hydroxybutyrate (ahba) side chain, which protects the antibiotic from several common resistance mechanisms. the ahba side chain is advantageously incorporated into clinically valuable antibiotics such as amikacin and arbekacin by synthetic methods. therefore, it is of significant interest to explore the biosynthetic origins of this useful moiety. we report here that the ahba side chain of butir ... | 2007 | 17462573 |
| a novel cyclic isomaltooligosaccharide (cycloisomaltodecaose, ci-10) produced by bacillus circulans t-3040 displays remarkable inclusion ability compared with cyclodextrins. | cyclodextrans (cis) are cyclic isomaltooligosaccharides and only ci-7, ci-8, and ci-9 were known. ci-7, ci-8, and ci-9, consisting of seven, eight, and nine glucoses, respectively, bound by alpha-(1-->6) linkages, are known to be produced by t-3040 strain of bacillus circulans. however, we have found, using 13c nmr and mass spectrometry, that this strain also produces ci-10, ci-11 and ci-12. these large cis are very soluble in water and inhibit the glucan synthesis of glucansucrases to the same ... | 2007 | 17433485 |
| efficient production of 2-deoxy-scyllo-inosose from d-glucose by metabolically engineered recombinant escherichia coli. | 2-deoxy-scyllo-inosose (doi) is a six-membered carbocycle formed from d-glucose-6-phosphate catalyzed by 2-deoxy-scyllo-inosose synthase (dois), a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics. doi is valuable as a starting material for the benzene-free synthesis of catechol and other benzenoids. we constructed a series of metabolically engineered escherichia coli strains by introducing a dois gene (btrc) from bacillus circulans and disrupting genes fo ... | 2007 | 17368605 |
| accurate, conformation-dependent predictions of solvent effects on protein ionization constants. | predicting how aqueous solvent modulates the conformational transitions and influences the pka values that regulate the biological functions of biomolecules remains an unsolved challenge. to address this problem, we developed fdpb_mf, a rotamer repacking method that exhaustively samples side chain conformational space and rigorously calculates multibody protein-solvent interactions. fdpb_mf predicts the effects on pka values of various solvent exposures, large ionic strength variations, strong e ... | 2007 | 17360348 |
| mutanase from a paenibacillus isolate: nucleotide sequence of the gene and properties of recombinant enzymes. | a mutanase (alpha-1,3-glucanase)-producing microorganism was isolated from a soil sample and was identified as a relative of paenibacillus sp. the mutanase was purified to homogeneity from culture, and its molecular mass was around 57 kda. the gene for the mutanase was cloned by pcr using primers based on the n-terminal amino acid sequence of the purified enzyme. the determined nucleotide sequence of the gene consisted of 3651-bp open reading frame that encoded a predicted 1217-amino acid polype ... | 2007 | 17270351 |
| characterization of the enzyme btrd from bacillus circulans and revision of its functional assignment in the biosynthesis of butirosin. | 2007 | 17226887 | |
| elaboration of neosamine rings in the biosynthesis of neomycin and butirosin. | the proteins neo-11 and neo-18 encoded in the neomycin gene cluster (neo) of streptomyces fradiae ncimb 8233 have been characterized as glucosaminyl-6'-oxidase and 6'-oxoglucosaminyl:l-glutamate aminotransferase, respectively. the joint activity of neo-11 and neo-18 is responsible for the conversion of paromamine to neamine in the biosynthetic pathway of neomycin through a mechanism of fad-dependent dehydrogenation followed by a pyridoxal-5'-phosphate-mediated transamination. neo-18 is also show ... | 2007 | 17206729 |
| cyclodextrin production by cyclodextrin glycosyltransferase from bacillus circulans df 9r. | cyclodextrins (cd) are cyclic oligosaccharides with multiple applications in the food, pharmaceutical, cosmetic, agricultural and chemical industries. in this work, the conditions used to produce cd with cyclodextrin glycosyltransferase from bacillus circulans df 9r were optimized using experimental designs. the developed method allowed the partial purification and concentration of the enzyme from the cultural broth and, subsequently, the cd production, using the same cassava starch as enzyme ad ... | 2007 | 17174549 |
| production of isocyclomaltopentaose from starch using isocyclomaltooligosaccharide glucanotransferase. | production of a novel cyclomaltopentaose cyclized by an alpha-1,6-linkage, [icg5; cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}], from starch was performed using isocyclomaltooligosaccharide glucanotransferase (igtase) derived from bacillus circulans am7. the optimal conditions for icg5-production from partially hydrolyzed starch were as follows: substrate concentration, 1.0% (w/v); ph, 5.5; temperature, 45 degrees c; reactio ... | 2006 | 17151467 |
| reduction of starch granule size by expression of an engineered tandem starch-binding domain in potato plants. | granule size is an important parameter when using starch in industrial applications. an artificial tandem repeat of a family 20 starch-binding domain (sbd2) was engineered by two copies of the sbd derived from bacillus circulans cyclodextrin glycosyltransferase via the pro-thr-rich linker peptide from xyn10a from cellulomonas fimi. sbd2 and a single sbd were introduced into the amylose-free potato mutant, amf, using appropriate signal sequences. the accumulation of sbd2 into transgenic starch gr ... | 2004 | 17147616 |
| mechanism of stabilization of bacillus circulans xylanase upon the introduction of disulfide bonds. | the introduction of disulfide bonds has been used as a strategy to enhance the stability of bacillus circulans xylanase. the transition temperature of the s100c/n148c (ds1), v98c/a152c (ds2), and a1gc/g187,c188 (cxl) in comparison to the wild type was increased by 5.0, 4.1 and 3.8 degrees c, respectively. interestingly, a combination of two disulfide bonds of ds1 and cxl (cds1, circular disulfide 1) led to a 12 degrees c increase in the transition temperature. importantly, an increase in the mel ... | 2007 | 17141401 |
| crystallization and preliminary x-ray analysis of the catalytic domain of chitinase d from bacillus circulans wl-12. | we report here on crystallization and preliminary x-ray analysis of the catalytic domain of chitinase d from bacillus circulans wl-12. the native crystals of this domain were found to belong to the orthorhombic space group p2(1)2(1)2(1). to elucidate the structure of the catalytic domain by the multiple isomorphous replacement method, 30 kinds of derivatized crystals were prepared by soaking the native crystals into a mother liquor containing salts of heavy metal atoms. difference patterson maps ... | 2006 | 17100652 |
| cloning, sequencing, and expression of the genes encoding an isocyclomaltooligosaccharide glucanotransferase and an alpha-amylase from a bacillus circulans strain. | the gene for a novel glucanotransferase, isocyclomaltooligosaccharide glucanotransferase (igty), involved in the synthesis of a cyclomaltopentaose cyclized by an alpha-1,6-linkage [icg5; cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}] from starch, was cloned from the genome of b. circulans am7. the igty gene, designated igty, consisted of 2,985 bp encoding a signal peptide of 35 amino acids and a mature protein of 960 amino ac ... | 2006 | 17090949 |
| role of glutamate 243 in the active site of 2-deoxy-scyllo-inosose synthase from bacillus circulans. | 2-deoxy-scyllo-inosose (doi) synthase is involved in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics and catalyzes the carbocyclic formation from d-glucose-6-phosphate (g-6-p) into doi. the reaction mechanism is proposed to be similar to that of dehydroquinate (dhq) synthase in the shikimate pathway, and includes oxidation of c-4, beta-elimination of phosphate, reduction of c-4, ring opening, and intramolecular aldol cyclization. to investigate the reaction mechanism ... | 2007 | 17035031 |
| purification, characterization, and gene analysis of cellulase (cel8a) from lysobacter sp. ib-9374. | an enzyme that has both beta-1,4-glucanase and chitosanase activities was found in the culture medium of the soil bacterium lysobacter sp. ib-9374, a high lysyl endopeptidase-producing strain. the enzyme was purified to homogeneity from the culture filtrate using five purification steps and designated cel8a. the purified cel8a had a molecular mass of 41 kda, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. a ph optimum of 5.0 was found for the beta-1,4-glucanase activit ... | 2006 | 17031054 |
| bacteriocins reduce campylobacter colonization and alter gut morphology in turkey poults. | campylobacter is a leading cause of food-borne illness in the united states. recent evidence has demonstrated that bacteriocins produced by bacillus circulans and paenibacillus polymyxa reduce cecal campylobacter colonization in broiler chickens infected with campylobacter jejuni. as campylobacter coli is the most prevalent campylobacter isolate recovered in turkeys, the objectives of the present study were to evaluate the efficacy of these bacteriocins against c. coli colonization and their inf ... | 2006 | 16977842 |
| a novel glucanotransferase from a bacillus circulans strain that produces a cyclomaltopentaose cyclized by an alpha-1,6-linkage. | a novel glucanotransferase, involved in the synthesis of a cyclomaltopentaose cyclized by an alpha-1,6-linkage [icg5; cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}], from starch, was purified to homogeneity from the culture supernatant of bacillus circulans am7. the pi was estimated to be 7.5. the molecular mass of the enzyme was estimated to be 184 kda by gel filtration and 106 kda by sds-page. these results suggest that the ... | 2006 | 16926508 |
| identification of catalytic amino acids of cyclodextran glucanotransferase from bacillus circulans t-3040. | in glycoside hydrolase family 66 (see http://afmb.cnrs-mrs.fr/cazy/), cyclodextran glucanotransferase (citase) is the only transglycosylation enzyme, all the other family 66 enzymes being dextranases. to analyze the catalytic amino acids of citase, we modified citase chemically from the t-3040 strain of bacillus circulans with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (edc). edc inactivated the enzyme by following pseudo-first order kinetics. in addition, the substrates of an isomaltooligos ... | 2006 | 16926507 |
| structure of full-length bacterial chitinase containing two fibronectin type iii domains revealed by small angle x-ray scattering. | chitinase a1 (chia1) from bacillus circulans wl-12 consists of an n-terminal catalytic domain, two fibronectin type iii domains (fniiids), and a c-terminal chitin-binding domain. the full-length structure of chia1 was studied by small angle x-ray scattering. the obtained low-resolution structure showed that chia1 is an elongated molecule with a length of approximately 145 a composed of a large globular head and a rod-like tail. combination with known high-resolution structures of individual chia ... | 2006 | 16899221 |
| crystal structures of the plp- and pmp-bound forms of btrr, a dual functional aminotransferase involved in butirosin biosynthesis. | the aminotransferase (btrr), which is involved in the biosynthesis of butirosin, a 2-deoxystreptamine (2-dos)-containing aminoglycoside antibiotic produced by bacillus circulans, catalyses the pyridoxal phosphate (plp)-dependent transamination reaction both of 2-deoxy-scyllo-inosose to 2-deoxy-scyllo-inosamine and of amino-dideoxy-scyllo-inosose to 2-dos. the high-resolution crystal structures of the plp- and pmp-bound forms of btrr aminotransferase from b. circulans were solved at resolutions o ... | 2006 | 16894611 |
| cloning and expression of an alpha-1,3-glucanase gene from bacillus circulans ka-304: the enzyme participates in protoplast formation of schizophyllum commune. | a culture filtrate of bacillus circulans ka-304 grown on a cell-wall preparation of schizophyllum commune has an activity to form protoplasts from s. commune mycelia, and a combination of alpha-1,3-glucanase and chitinase i, which were isolated from the filtrate, brings about the protoplast-forming activity. the gene of alpha-1,3-glucanase was cloned from b. circulans ka-304. it consists of 3,879 nucleotides, which encodes 1,293 amino acids including a putative signal peptide (31 amino acid resi ... | 2006 | 16861810 |
| bacillus anthracis virulent plasmid px02 genes found in large plasmids of two other bacillus species. | in order to cause the disease anthrax, bacillus anthracis requires two plasmids, px01 and px02, which carry toxin and capsule genes, respectively, that are used as genetic targets in the laboratory detection of the bacterium. clinical, forensic, and environmental samples that test positive by pcr protocols established by the centers for disease control and prevention for b. anthracis are considered to be potentially b. anthracis until confirmed by culture and a secondary battery of tests. we rep ... | 2006 | 16825351 |
| bioremediation of endosulfan contaminated soil and water -- optimization of operating conditions in laboratory scale reactors. | a mixed bacterial culture consisted of staphylococcus sp., bacillus circulans-i and -ii has been enriched from contaminated soil collected from the vicinity of an endosulfan processing industry. the degradation of endosulfan by mixed bacterial culture was studied in aerobic and facultative anaerobic conditions via batch experiments with an initial endosulfan concentration of 50mg/l. after 3 weeks of incubation, mixed bacterial culture was able to degrade 71.58+/-0.2% and 75.88+/-0.2% of endosulf ... | 2006 | 16730891 |
| enzymatic production of highly soluble myricitrin glycosides using beta-galactosidase. | myricitrin, a botanical flavonol glycoside, could be a useful ingredient of functional foods, cosmetics, and medicines because of its high anti-oxidative activity. however, due to its insolubility in water, it has a limited range of use. to improve this solubility, we glycosylated myricitrin by an enzymatic transglycosylate reaction. myricitrin was galactosylated by beta-galactosidase from bacillus circulans using lactose as a sugar donor. the reaction product was 480 times more soluble than myr ... | 2006 | 16636462 |
| anti-apoptotic activity of laminarin polysaccharides and their enzymatically hydrolyzed oligosaccharides from laminaria japonica. | laminarin polysaccharides (lp1) were prepared from laminaria japonica, a marine brown alga with potential biological activities, by hot water extraction, ultrafiltration and gel chromatography; the molecular weights of the lp1s were between 5 and 10 kda. laminarin oligosaccharides (lo) derived by hydrolyzing lp1 with an endo-beta-(1-->3)-glucanase from bacillus circulans were mainly di- and penta-oligosaccharides. treatment of mouse thymocytes with lo or lp1 (1-4 mg ml(-1)) suppressed apoptotic ... | 2006 | 16614911 |
| identification of the substrate interaction region of the chitin-binding domain of streptomyces griseus chitinase c. | chitinase c from streptomyces griseus hut6037 was discovered as the first bacterial chitinase in family 19 other than chitinases found in higher plants. chitinase c comprises two domains: a chitin-binding domain (chbd(chic)) for attachment to chitin and a chitin-catalytic domain for digesting chitin. the structure of chbd(chic) was determined by means of 13c-, 15n-, and 1h-resonance nuclear magnetic resonance (nmr) spectroscopy. the conformation of its backbone comprised two beta-sheets composed ... | 2006 | 16567413 |
| on the naming of two antibiotics from members of the bacillus circulans group: circulin and polypeptin. | 1949 | 16561756 | |
| circulin an antibiotic from an organism resembling bacillus circulans. | 1949 | 16561679 | |
| circulin, an antibiotic from a member of the bacillus circulans group: i. bacteriological studies. | 1948 | 16561627 | |
| an enzymatically produced novel cyclomaltopentaose cyclized from amylose by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}. | a bacterial strain am7, isolated from soil and identified as bacillus circulans, produced two kinds of novel cyclic oligosaccharides. the cyclic oligosaccharides were produced from amylose using a culture supernatant of the strain as the enzyme preparation. the major product was a cyclomaltopentaose cyclized by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}. the other minor product was cyclomaltohexao ... | 2006 | 16545346 |
| effect of ph on the structure and stability of bacillus circulans ssp. alkalophilus phosphoserine aminotransferase: thermodynamic and crystallographic studies. | ph is one of the key parameters that affect the stability and function of proteins. we have studied the effect of ph on the pyridoxal-5'-phosphate-dependent enzyme phosphoserine aminotransferase produced by the facultative alkaliphile bacillus circulans ssp. alkalophilus using thermodynamic and crystallographic analysis. enzymatic activity assay showed that the enzyme has maximum activity at ph 9.0 and relative activity less than 10% at ph 7.0. differential scanning calorimetry and circular dich ... | 2006 | 16532449 |
| beneficiation of iron ore slime using aspergillus niger and bacillus circulans. | studies were carried out on the removal of alumina from iron ore slime containing (%) fe(2)o(3) 75.7, al(2)o(3) 9.95, sio(2) 6.1, fe (total) 52.94 with the help of bacillus circulans and aspergillus niger. b. circulans and a. niger showed 39% and 38% alumina removal after six and 15 days of in situ leaching at 10% pulp density, respectively. culture filtrate leaching with a. niger removed 20% alumina at 2% pulp density with 13 day old culture filtrate. b. circulans was more efficient than a. nig ... | 2006 | 16531043 |
| engineering of cyclodextrin glucanotransferase on the cell surface of saccharomyces cerevisiae for improved cyclodextrin production. | the cyclodextrin glucanotransferase (cgtase) gene (cgt) from bacillus circulans 251 was cloned into plasmid pyd1, which allowed regulated expression, secretion, and detection. the expression of cgtase with a-agglutinin at the n-terminal end on the extracellular surface of saccharomyces cerevisiae was confirmed by immunofluorescence microscopy. this surface-anchored cgtase gave the yeast the ability to directly utilize starch as a sole carbon source and the ability to produce the anticipated prod ... | 2006 | 16517633 |
| mapping of residues involved in the interaction between the bacillus subtilis xylanase a and proteinaceous wheat xylanase inhibitors. | the bacillus subtilis xylanase a was subjected to site-directed mutagenesis, aimed at changing the interaction with triticum aestivum xylanase inhibitor, the only wheat endogenous proteinaceous xylanase inhibitor interacting with this xylanase. the published structure of bacillus circulans xyna was used to target amino acids surrounding the active site cleft of b.subtilis xyna for mutation. twenty-two residues were mutated, resulting in 62 different variants. the catalytic activity of active mut ... | 2006 | 16517552 |
| crystallization and x-ray analysis of 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics. | a recombinant 2-deoxy-scyllo-inosose synthase from bacillus circulans has been crystallized at 277 k using peg 4000 as precipitant. the diffraction pattern of the crystal extends to 2.30 a resolution at 100 k using synchrotron radiation at the photon factory. the crystals are monoclinic and belong to space group p2(1), with unit-cell parameters a = 80.5, b = 70.4, c = 83.0 a, beta = 117.8 degrees. the presence of two molecules per asymmetric unit gives a crystal volume per protein weight (vm) of ... | 2005 | 16511136 |
| enrichment and isolation of a mixed bacterial culture for complete mineralization of endosulfan. | in the present study, we isolated three novel bacterial species, namely, staphylococcus sp., bacillus circulans-i, and bacillus circulans-ii, from contaminated soil collected from the premises of a pesticide manufacturing industry. batch experiments were conducted using both mixed and pure cultures to assess their potential for the degradation of aqueous endosulfan in aerobic and facultative anaerobic condition. the influence of supplementary carbon (dextrose) source on endosulfan degradation wa ... | 2006 | 16393897 |
| production of lewis x tetrasaccharides by metabolically engineered escherichia coli. | two tetrasaccharides carrying the trisaccharidic lewis x motif on a glcnac or a gal residue were produced on the gram-scale by high-cell-density cultures of metabolically engineered escherichia coli strains that overexpressed the helicobacter pylori futa gene for alpha-3 fucosyltransferase and the neisseria meningitidis lgtb gene for beta-4 galactosyltransferase. the first compound galbeta-4(fucalpha-3)glcnacbeta-4glcnac was produced by glycosylation of chitinbiose, which was endogenously genera ... | 2006 | 16381046 |
| isolation of pathogenic bacillus circulans from callus cultures and healthy offshoots of date palm (phoenix dactylifera l.). | pure cultures of a gram-negative, rod-shaped bacterium, which produced endospores after 3 to 5 days on solid medium, were isolated exclusively from tissue cultures of the date palm phoenix dactylifera l. electron microscopic examination of thin sections of the bacteria revealed the bilayer membrane typical of gramnegative bacteria and confirmed the nature of the spores as true endospores. biochemical and physiological tests indicated that the bacteria were bacillus circulans. b. circulans was co ... | 1986 | 16347217 |
| distribution of alginate lyase activity among strains of bacillus circulans. | strains from four different dna relatedness groups of bacillus circulans showed apparent alginate lyase activity; the activity of three strains examined had mannuronidase specificity. a representative strain of group 4 also produced apparent inducible unsulfated chrondroitin lyase activity. | 1985 | 16346766 |
| bacillus circulans mh-k1 chitosanase: amino acid residues responsible for substrate binding. | to identify the amino acids responsible for the substrate binding of chitosanase from bacillus circulans mh-k1 (mh-k1 chitosanase), tyr148 and lys218 of the chitosanase were mutated to serine and proline, respectively, and the mutated chitosanases were characterized. the enzymatic activities of y148s and k218p were found to be 12.5% and 0.16% of the wild type, respectively. when the (glcn)3 binding ability to the chitosanase was evaluated by fluorescence spectroscopy and thermal unfolding experi ... | 2005 | 16272568 |
| characterization of bacteriocin n15 produced by enterococcus faecium n15 and cloning of the related genes. | enterococcus faecium n15 was isolated from nuka (japanese rice-bran paste), which is utilized as starter in the fermenting of vegetables, and was found to produce a bacteriocin that exhibited a broad spectrum of activity, including activity against listeria monocytogenes and bacillus circulans jcm2504. the bacteriocin was sensitive to proteases (alpha-chymotrypsin, proteinase k, trypsin, and pepsin) and alpha-amylase, but it was resistant to lipase. the bacteriocin was resistant to heat treatmen ... | 2001 | 16233010 |
| cloning and sequencing of the deacetylase gene from vibrio alginolyticus h-8. | a gene encoding deacetylase da1 that is specific for n, n'-diacetylchitobiose was cloned using the shot-gun method with puc118 and sequenced. the open reading frame encoded a protein of 427 amino acids including the signal peptide. the molecular mass of the mature enzyme estimated from the amino acid sequence data was 44.7 kda, which is approximately similar to that, estimated by sds-page (48.0 kda), of the purified enzyme reported previously. the n-terminal amino acid sequence deduced from the ... | 2000 | 16232910 |
| alteration of product specificity of cyclodextrin glucanotransferase from thermococcus sp. b1001 by site-directed mutagenesis. | cyclodextrin glucanotransferase (cgtase) from the hyperthermophilic archaeon thermococcus sp. b1001 catalyzed the production predominantly of alpha-cyclodextrin (cd) from starch (tachibana, y. et al., appl. environ. microbiol., 65, 1991-1997, 1999). the cgtase gene (cgta) from this strain was cloned and sequenced. it was composed of 2217 nucleotides, and encoded a protein (739 amino acids) with a molecular mass of 83,240 da. recombinant cgta expressed in escherichia coli also catalyzed the produ ... | 2000 | 16232729 |
| site-directed mutagenesis of asp280 suggests substrate-assisted catalysis of chitinase a1 from bacillus circulans wl-12. | to study the involvement of asp280 of chitinase a1 from bacillus circulans wl-12 in the catalytic reaction of the enzyme, site-directed mutagenesis of this residue was carried out. kinetic analysis of the mutant chitinases revealed that the residue is not absolutely essential for the catalytic reaction and thus, does not act as the catalytic nucleophile. | 2000 | 16232709 |
| purification and gene cloning of a chitosanase from bacillus ehimensis eag1. | bacillus ehimensis eag1 (ifo15659) produced and secreted chitosanase in the presence of exogenous chitosan. the chitosanase was purified from the culture filtrate of the bacterium to apparent homogeneity in sds-polyacrylamide gel electrophoresis. the purified enzyme had a molecular weight of approximately 31,000. a 1.9-kbp dna fragment containing the chitosanase gene was cloned and the complete nucleotide sequence was determined. the sequence was found to contain a single open reading frame enco ... | 1999 | 16232486 |
| efficient enzymatic synthesis of 4-methylumbelliferyl n-acetyllactosaminide and 4-methylumbelliferyl sialyl n-acetyllactosaminides employing beta-d-galactosidase and sialyltransferases. | 4-methylumbelliferyl n-acetyllactosaminide and 4-methylumbelliferyl sialyl n-acetyllactosaminides, which are used for the assay of sialytransferase, neuraminidase and fucosyltransferase, were synthesized, respectively, by the beta-d: -galactosidase from bacillus circulans and by a recombinant rat alpha2,3-(n)-sialyltransferase or rat liver alpha2,6-(n)-sialyltransferase with cmp-n-acetylneuraminic acid as donor. | 2005 | 16231217 |
| statistical optimization of thermo-tolerant xylanase activity from amazon isolated bacillus circulans on solid-state cultivation. | a 2(2) factorial design was performed to find the best conditions of ph and temperature for xylanolytic activity of bacillus circulans bl53 isolated from the amazon environment. solid-state cultivation was carried out on an inexpensive, abundant agro-industrial soybean residue. the central composite design (ccd) used for the analysis of treatment combinations showed that a second-order polynomial regression model was in good agreement with experimental results, with r(2) = 0.9369 (p < 0.05). the ... | 2006 | 16216495 |
| characterisation of the microflora of attiéké, a fermented cassava product, during traditional small-scale preparation. | attiéké is a fermented cassava product consumed mainly in cote d'ivoire. the aim of this study was to characterise the attiéké fermentation by examining products from 15 small-scale production sites at various stages of its preparation. for the preparation of attiéké, fresh cassava is grated to a pulp and inoculated with 10% of a spontaneous traditional inoculum. the inocula contained aerobic mesophiles at mean numbers of 8.2 x 10(7) cfu/g and lactic and acetic acids at mean concentrations of 0. ... | 2006 | 16213052 |
| alkaline protease production by an isolated bacillus circulans under solid-state fermentation using agroindustrial waste: process parameters optimization. | alkaline protease production using isolated bacillus circulans under solid-state fermentation environment was optimized by using taguchi orthogonal array (oa) experimental design (doe) methodology to understand the interaction of a large number of variables spanned by factors and their settings with a small number of experiments in order to economize the process optimization. the software-designed experiments with an oa worksheet of l-27 was selected to optimize fermentation (temperature, partic ... | 2005 | 16209541 |
| convenient enzymatic synthesis of a p-nitrophenyl oligosaccharide series of sialyl n-acetyllactosamine, sialyl le x and relevant compounds. | from the beta-d-gal-(1-->4)-beta-d-glcnac-oc6h4no2-p (1) prepared by the transglycosylation of beta-galactosidase from bacillus circulans, alpha-d-neu5ac-(2-->3)-beta-d-gal-(1-->4)-beta-d-glcnac-oc6h4no2-p (9) and alpha-d-neu5ac-(2-->6)-beta-d-gal-(1-->4)-beta-d-glcnac-oc6h4no2-p (10) were effectively synthesized with an equimolar ratio of cmp-neu5ac by recombinant rat alpha-(2-->3)-n-sialyltransferase and rat liver alpha-(2-->6)-n-sialyltransferase, respectively. the former enzyme also transfer ... | 2005 | 16169536 |
| rapid affinity purification processes for cyclodextrin glycosyltransferase from bacillus circulans. | two rapid and easy-to-scale-up methods for the purification of cyclodextrin glycosyltransferase (cgtase) from bacillus circulans were developed: affinity precipitation with starch and aqueous two-phase partition. the first method, optimised by a factorial design, gave an 80% cgtase adsorption at 11% starch and 1.6% ammonium sulphate, and a 65% recovery after elution with 10 mm alpha-cyclodextrin. the purification factor was 17. aqueous two-phase partition yielded a 72% cgtase recovery in a two-s ... | 2005 | 16158259 |
| extended sequence and functional analysis of the butirosin biosynthetic gene cluster in bacillus circulans sank 72073. | butirosin produced by bacillus circulans is among the clinically important 2-deoxystreptamine containing aminoglycoside antibiotics and its unique structure is found in (s)-4-amino-2-hydroxyburyric acid substituted at c-1 of 2-deoxystreptamine. recently, the key part of the butirosin biosynthetic gene cluster has been identified from bacillus circulans sank 72073, however the whole gene for the biosynthesis awaited for identification. in the present study, we undertook extended analysis of the b ... | 2005 | 16156513 |
| construction of chimeric cyclodextrin glucanotransferases from bacillus circulans a11 and paenibacillus macerans iam1243 and analysis of their product specificity. | three dna fragments of 7919 base pairs containing genes for beta-cyclodextrin glucanotransferase (cgtase, ec 2.4.1.19), an iron iii dicitrate transport protein-like protein and a partial coding sequence for putative ferrichrome abc transporter from bacillus circulans a11 were cloned and sequenced (genbank accession af302787). the dna sequence contained a cgtase open reading frame of 2139 base pairs, which encoded a polypeptide of 713 amino acid residues. the signal peptide constituted the n-term ... | 2005 | 16084934 |
| chitin-binding domain based immobilization of d-hydantoinase. | chitin-binding domain (chbd) of chitinase a1 from bacillus circulans wl-12 comprises 45 amino acids and exhibits remarkably high specificity to chitin (hashimoto, m., ikegami, t., seino, s., ohuchi, n., fukada, h., sugiyama, j., shirakawa, m., watanabe, t., 2000. expression and characterization of the chitin-binding domain of chintinase a1 from b. circulans wl-12. j. bacteriol. 182, 3045-3054.). to investigate the feasibility of exploiting chbd as affinity tags to confine enzymes of interest on ... | 2005 | 15862357 |
| cloning and expression of a bacillus circulans ka-304 gene encoding chitinase i, which participates in protoplast formation of schizophyllum commune. | ka-prep, a culture filtrate of bacillus circulans ka-304 grown on a cell-wall preparation of schizophyllum commune, has an activity to form protoplasts from s. commune mycelia, and a combination of alpha-1,3-glucanase and chitinase i, isolated from ka-prep, brings about the protoplast-forming activity. the gene of chitinase i was cloned from b. circulans ka-304 into pgem-t easy vector. the gene consists of 1,239 nucleotides, which encodes 413 amino acids including a putative signal peptide (24 a ... | 2005 | 15784990 |
| isolation of bacillus circulans and paenibacillus polymyxa strains inhibitory to campylobacter jejuni and characterization of associated bacteriocins. | we evaluated anti-campylobacter activity among 365 bacillus and paenibacillus isolates from poultry production environments. one novel antagonistic bacillus circulans and three paenibacillus polymyxa strains were identified and further studied. cell-free ammonium sulfate precipitate (crude antimicrobial preparation) was obtained from each candidate culture. zones of campylobacter growth inhibition surrounding 10 microl of this crude antimicrobial preparation were quantified using a spot test. ca ... | 2005 | 15690798 |
| enzyme adaptation to alkaline ph: atomic resolution (1.08 a) structure of phosphoserine aminotransferase from bacillus alcalophilus. | the crystal structure of the vitamin b(6)-dependent enzyme phosphoserine aminotransferase from the obligatory alkaliphile bacillus alcalophilus has been determined at 1.08 a resolution. the model was refined to an r-factor of 11.7% (r(free) = 13.9%). the enzyme displays a narrow ph optimum of enzymatic activity at ph 9.0. the final structure was compared to the previously reported structure of the mesophilic phosphoserine aminotransferase from escherichia coli and to that of phosphoserine aminot ... | 2005 | 15608117 |
| over-expression of the gene (bglbc1) from bacillus circulans encoding an endo-beta-(1-->3),(1-->4)-glucanase useful for the preparation of oligosaccharides from barley beta-glucan. | an endo-beta-(1-->3),(1-->4)-glucanase gene (bglbc1) from bacillus circulans atcc21367 was modified by substituting its native promoter with a strong promoter, bj27x, to increase expression of the gene when cloned into b. subtilis rm125 and b. megaterium atcc14945. a 771-bp endo-beta-(1-->3),(1-->4)-glucanase open reading frame was inserted into a new shuttle plasmid, pblc771, by ligating the orf and pbe1, the latter of which contained the strong promoter, bj27x. b. subtilis , transformed with t ... | 2004 | 15604830 |
| cloning, expression, and characterization of a highly thermostable family 18 chitinase from rhodothermus marinus. | a family 18 chitinase gene chia from the thermophile rhodothermus marinus was cloned and expressed in escherichia coli. the gene consisted of an open reading frame of 1,131 nucleotides encoding a protein of 377 amino acids with a calculated molecular weight of 42,341 da. the deduced chia was a non-modular enzyme with one unique glycoside hydrolase family 18 catalytic domain. the catalytic domain exhibited 43% amino acid identity with bacillus circulans chitinase c. due to poor expression of chia ... | 2004 | 15583965 |
| regioselectivity in beta-galactosidase-catalyzed transglycosylation for the enzymatic assembly of d-galactosyl-d-mannose. | the regioselectivity of beta-galactosidase derived from bacillus circulans atcc 31382 (beta-1,3-galactosidase) in transgalactosylation reactions using d-mannose as an acceptor was investigated. this d-mannose associated regioselectivity was found to be different from reactions using either glcnac or galnac as acceptors, not only for beta-1,3-galactosidase but also for beta-galactosidases of different origins. the relative hydrolysis rate of gal beta-pnp and d-galactosyl-d-mannoses, of various li ... | 2004 | 15502353 |
| bacillus circulans peritonitis in a patient treated with capd. | 2004 | 15490992 | |
| use of heterotrophic co2 assimilation as a measure of metabolic activity in planktonic and sessile bacteria. | we have examined whether assimilation of co2 can be used as a measure of metabolic activity in planktonic and sessile heterotrophic bacteria. co2 assimilation by environmental samples and pure cultures of heterotrophic bacteria was studied using 14co2 and 13co2 as tracers. heterotrophic growth on complex organic substrates resulted in assimilation of co2 into cell biomass by activated sludge, drinking water biofilm, and pure cultures of escherichia coli atcc 25922, es. coli atcc 13706, rhodococc ... | 2004 | 15488281 |
| rhizosheath of sinai desert plants is a potential repository for associative diazotrophs. | among 42 plant species representing the flora of north sinai, two possessed sand grain sheath encasing the roots. they are panicum turgidum forssk. and stipagrostis scoparia (trin.and rupr.) dewinter. rhizosheaths, compared to surrounding free sand, accommodated higher population density of microorganisms including associative diazotrophs. isolates secured belonged to the species of bacillus circulans, paenib. macerans (bacillus macerans), enterobacter agglomerans, agrobacterium radiobacter and ... | 2004 | 15462528 |
| identification of an alkaline-tolerant cyclodextrin-metabolizing bacterium and characterization of its cyclodextrinase gene. | bacillus circulans a11, an alkaline-tolerant cyclodextrin-metabolizing bacterium isolated from south-east asian soil, was reidentified as paenibacillus sp. a11 based on 16s rrna gene sequence comparison, g + c content and cellular fatty acid composition. levels of similarity of the 16s rrna gene between strain a11 and the paenibacillus species were 90-99%, while similarity with bacillus circulans was only 86%. the major cellular fatty acid was anteiso-c15:0 which accounted for 59.3% of the total ... | 2004 | 15378529 |
| gene cluster in micromonospora echinospora atcc15835 for the biosynthesis of the gentamicin c complex. | gentamicin is a 4,6-disubstituted aminocyclitol antibiotic complex synthesised by some members of the actinomycete genus micromonospora. in a search for the gentamicin biosynthetic gene cluster we identified, using a cosmid library approach, a region of the m. echinospora atcc15835 chromosome that encodes homologues of aminoglycoside biosynthesis genes including gntb-a close homologue of the 2-deoxy-scyllo-inosose synthase gene (btrc) from butirosin-producing bacillus circulans. insertional inac ... | 2004 | 15376556 |
| mechanism-based fluorescent labeling of beta-galactosidases. an efficient method in proteomics for glycoside hydrolases. | (4-n-5-dimethylaminonaphthalene-1-sulfonyl-2-difluoromethylphenyl)-beta-d-galactopyranoside was synthesized and successfully tested on beta-galactosidases from xanthomonas manihotis (wong-madden, s. t., and landry, d. glycobiology (1995) 5, 19-28 and taron, c. h., benner, j. s., hornstra, l. j., and guthrie, e. p. (1995) glycobiology 5, 603-610), escherichia coli (jacobson, r. h., zhang, x. j., dubose, r. f., and matthews, b. w. (1994) nature 369, 761-766), and bacillus circulans (fujimoto, h., ... | 2004 | 15308675 |
| crystallization and preliminary x-ray crystallographic analysis of chitosanase from bacillus circulans mh-k1. | chitosanase, an enzyme which hydrolyzes chitosan, isolated from bacillus circulans mh-k1, was crystallized by a vapor-diffusion procedure at 293 k using ammonium sulfate as a precipitant. rod-shaped colorless crystals, which grew to 0.05 x 0.15 x 1.2 mm within a week, belong to the orthorhombic system and the space group p222(1) or p2(1)2(1)2 with unit-cell dimensions of a = 43.3, b = 57.7, and c = 128.0 a. the asymmetric unit is thought to contain one chitosanase molecule (29 024 da). the cryst ... | 1995 | 15299827 |
| two-step purification of bacillus circulans chitinase a1 expressed in escherichia coli periplasm. | a protein purification procedure was developed to efficiently and effectively purify the target enzyme, chitinase a1 of bacillus circulans wl-12, from escherichia coli dh5alpha carrying the chia gene with its natural promoter in the plasmid pntu110. chitinase a1 was purified to apparent homogeneity from e. coli periplasm with a final recovery of 90.6%. two main steps were included in this protein purification procedure, ammonium sulfate precipitation (40% saturation) and anion-exchange chromatog ... | 2004 | 15294277 |
| enhancement of the antifungal activity of bacillus subtilis f29-3 by the chitinase encoded by bacillus circulans chia gene. | bacillus subtilis f29-3 is an antagonistic bacterium against a wide range of fungal species. in order to determine the effect of chitinase on the antifungal activity of b. subtilis f29-3, a 2.4-kb dna fragment containing the chia gene of bacillus circulans wl-12 was ligated into a shuttle vector phy300plk and transformed into b. subtilis f29-3. a bioassay conducted on the culture supernatant showed that, in comparison to the b. subtilis control strain, b. subtilis f29-3 expressing the chia gene ... | 2004 | 15284891 |
| evaluation of fly ash as a carrier for diazotrophs and phosphobacteria. | fly ash and its different combinations with soil (w/w) were tested to explore its possible use as a potential carrier for diazotrophs and phosphobacteria. azotobacter chroococcum, azospirillum brasilense and bacillus circulans showed their maximum viability in fly ash alone whereas pseudomonas striata proliferated most in soil:fly ash (1:1) combination. | 2004 | 15246443 |