acetylcholinesterases from musca domestica and drosophila melanogaster brain are linked to membranes by a glycophospholipid anchor sensitive to an endogenous phospholipase.the sensitivity of acetylcholinesterases (aches) from musca domestica and from drosophila melanogaster to the phosphatidylinositol-specific phospholipase c from bacillus cereus and to the glycosylphosphatidylinositol-specific phospholipase c from trypanosoma brucei was investigated. b. cereus phospholipase c solubilizes membrane-bound ache, and both phospholipases convert amphiphilic aches into hydrophilic forms of the enzyme. the lipases uncover an immunological determinant that is found on oth ...19882831298
endovesiculation of human erythrocytes exposed to sphingomyelinase c: a possible explanation for the enzyme-resistant pool of sphingomyelin.when human erythrocytes are treated with staphylococcus aureus sphingomyelinase c at 37 degrees c they become susceptible to cold lysis and appear to endovesiculate. endovesiculation has been confirmed by showing that in parallel with sphingomyelin breakdown, the cells accumulate [3h]inulin or [14c]sucrose (without losing intracellular k+) and also experience a loss of cell-surface acetylcholinesterase activity into a latent intracellular pool which can be revealed by treatment with detergent. o ...19882831979
inactivation of blasticidin s by bacillus cereus. ii. isolation and characterization of a plasmid, pbsr8, from bacillus cereus. 19882833485
bcefi, a new type iis restriction endonuclease.a new type iis restriction endonuclease was identified, partially purified and characterized from a bacillus cereus subsp. fluorescens strain. the enzyme recognizes the nonpalindromic sequence acggc and cleaves at a distance from it. the cleavage appears to occur with a +/- 1 basepair uncertainty. thus the cleavage and recognition site is as shown below: acggc(n)11-13 tgccg(n)12-14.19882835751
near stoichiometric, irreversible inactivation of bacterial collagenases by o-chloranil (3,4,5,6-tetrachloro-1,2-benzoquinone).the hydrogen-abstracting quinone derivative 3,4,5,6-tetrachloro-1,2-benzoquinone (o-chloranil) caused a strong, near stoichiometric, irreversible inactivation of the collagenases from bacillus cereus, clostridium histolyticum and achromobacter iophagus. p-chloranil was a weaker inactivator. o-chloranil reacted rapidly with a site that affected substrate binding. amino acid analyses of native and totally inactivated enzymes, and the ph-profile of inactivation suggest that the dissociated form of ...19882837215
nucleotide sequence and expression in escherichia coli of the gene coding for sphingomyelinase of bacillus cereus.bacillus cereus secretes phospholipases c, which hydrolyze phosphatidylcholine, sphingomyelin and phosphatidylinositol. a 7.5-kb hindiii fragment of b. cereus dna cloned into escherichia coli, with puc18 as a vector, directed the synthesis of the sphingomyelin-hydrolyzing phospholipase c, sphingomyelinase. nucleotide sequence analysis of the subfragment revealed that it contained two open reading frames in tandem. the upstream truncated open reading frame corresponds to the carboxy-terminal port ...19882841128
the solubilization of tetrameric alkaline phosphatase from human liver and its conversion into various forms by phosphatidylinositol phospholipase c or proteolysis.when membrane-bound human liver alkaline phosphatase was treated with a phosphatidylinositol (pi) phospholipase c obtained from bacillus cereus, or with the proteases ficin and bromelain, the enzyme released was dimeric. butanol extraction of the plasma membranes at ph 7.6 yielded a water-soluble, aggregated form that pi phospholipase c could also convert to dimers. when the membrane-bound enzyme was solubilized with a non-ionic detergent (nonidet p-40), it had the mr of a tetramer; this, too, w ...19882844768
bacillus cereus strain se-1: nucleotide sequence of the sphingomyelinase c gene. 19882848222
inhibition of phospholipases by met-leu-phe-ile-leu-ile-lys-arg-ser-arg-his-phe, c terminus of middle-sized tumor antigen.the n and c terminals and tyrosine-phosphorylating site of the middle-sized tumor antigen of polyoma virus were chemically synthesized. the sequences of these peptides were met-asp-arg-val-leu-ser-arg-ala-asp-lys (n-mt), met-leu-phe-ile-leu-ile-lys-arg-ser-arg-his-phe (c-mt), and glu-glu-glu-glu-tyr-met-pro-met-glu (mt-tyr), respectively. among these peptides, the c-mt peptide inhibited phospholipase a2 (ec, phospholipase c (ec, and phospholipase d (ec in addition, ph ...19852857079
enterococci in lambs. 19852862641
regulation and properties of glutamine synthetase purified from bacillus cereus.the glutamine synthetase from bacillus cereus ifo 3131 was purified to homogeneity. the enzyme is a dodecamer with a molecular weight of approximately 600,000, and its subunit molecular weight is 50,000. both mg2+ and mn2+ activated the enzyme as to the biosynthesis of l-glutamine, but, unlike in the case of the e. coli enzyme, the mg2+-dependent activity was stimulated by the addition of mn2+. the highest activity was obtained when 20 mm mg2+ and 0.5 mm mn2+ were added to the assay mixture. for ...19852867996
identification of an essential glutamic acid residue in beta-lactamase ii from bacillus cereus.beta-lactamase ii from bacillus cereus was readily inactivated by incubation at ph 4.75 with a water-soluble carbodiimide plus a suitable nucleophile. in the early stages of the reaction, 1 equivalent of nucleophile was incorporated/equivalent of enzyme, whereas during the later stages a second equivalent of nucleophile was also incorporated. this latter process correlated with the blocking of the enzyme's single thiol group. enzyme inactivated in the presence of the coloured nucleophile n-(2,4- ...19862869754
microbiological quality of products used in enteral feeds.the microbiological quality of 19 commonly used nasogastric feeds and feed ingredients was determined. thirteen of the products yielded no viable micro-organisms while six, all of which were powders containing milk or whey proteins, gave aerobic viable counts from 50-3000 organisms g-1. possible microbial limits for enteral feeds are discussed with reference to those already suggested for special dietary products, infant formulas and non-sterile pharmaceuticals.19862870111
lethal effect of butylated hydroxyanisole as related to bacterial fatty acid composition.bacillus cereus, clostridium perfringens, staphylococcus aureus, pseudomonas fluorescens, pseudomonas fragi, escherichia coli, and salmonella "anatum" were challenged with butylated hydroxyanisole (bha). susceptibility was measured as the concentration of bha required to cause a 90% reduction in bacterial survivors. staphylococcus aureus lp and p. fragi were two of the most resistant species examined; c. perfringens and p. fluorescens were the most susceptible. gram stain reaction was found not ...19862873790
effects of phospholipases c on the beta-receptor-adenylate cyclase system of chick erythrocyte membranes.the beta-adrenergic receptor located in chick erythrocyte membranes was characterized using (-)-[3h]-dihydroalprenolol ([3h]-dha) with rapid filtration techniques. the affinity of beta-adrenergic antagonist, (-)-propranolol, was approximately 100-fold higher than that of (+)-propranolol. catecholamines were bound with the receptor in the following order, (-)-isoproterenol greater than (-)-norepinephrine greater than (-)-epinephrine, suggested the binding site to be beta 1-classification. when th ...19862875718
effects of intra- and extracellularly applied phospholipases c on excitability of squid giant axons.the effects of phospholipases c on the membrane excitability of the squid giant axon were investigated using phosphatidylcholine-hydrolyzing phospholipase c and sphingomyelinase c of bacillus cereus, and phosphatidylinositol-specific phospholipase c of bacillus thuringiensis. when the squid axon was perfused internally with phosphatidylcholine-hydrolyzing phospholipase c in kf or k-glutamate solution, the action potential was blocked in 4-7 min and membrane resistance decreased with time to a le ...19862877512
independent bindings of mn2+ and mg2+ to the active site of b. cereus glutamine synthetase.glutamine synthetase purified from bacillus cereus ifo 3131 was modified by iodoacetamide and the atp analog 5'-p-fluorosulfonylbenzoyladenosine (fsba). only mg2+-dependent activity was inactivated by iodoacetamide, whereas only mn2+-dependent activity was inactivated by fsba. when iodoacetamide-treated enzyme was reacted with fsba, mn2+-dependent activity was also inactivated. mg2+ plus mn2+-dependent activity was inactivated in any case. the results suggested that the binding sites of mn2+ and ...19872880587
synthesis of a thiophosphate analog of dioctanoylphosphatidylcholine: a phospholipase c substrate.dioctanoylthiophosphatidylcholine, a racemic thiophosphate analog of l-alpha-dioctanoylphosphatidylcholine, has been synthesized and isolated by flash chromatography. in contrast with the didecanoylthiophosphatidylcholine synthesized previously, the analog is easily dispersed on sonication in aqueous media and is rapidly hydrolyzed to produce a free thiol group in the presence of the extracellular phospholipase c from either bacillus cereus or clostridium perfringens. when 5,5'-dithiobis (2-nitr ...19872889786
egg yolk paste for determining some food poisoning bacteria.egg yolk, aseptically prepared from fresh eggs, was partially dehydrated with a 40% high fructose corn syrup solution, and 10% salt was added. this salted yolk paste was added to mannitol salt agar for the detection of staphylococcus aureus, to nacl-glycine kim and goepfert medium for detection of bacillus cereus, to clostridium welchii agar for detection of c. perfringens, and to gifu anaerobic medium for detection of c. botulinum. these food poisoning bacteria showed the same lecithovitellin ( ...19882898139
[microbial contamination of traditional herbal drugs in indonesia]. 19882907976
excretion of tetracycline and chlortetracycline in eggs after oral medication of laying hens.after dosing laying hens orally with tetracycline (tc) through either drinking water (0.25 and 0.5 g/l for 5 days) or feed (300 and 600 ppm for 7 days), and chlortetracycline (ctc) through feed (600 ppm) residues were determined by an agar plate diffusion technique in cylinders with bacillus cereus as test-organism, separately for albumen and for yolk. the sensitivity threshold was 0.07 micrograms/g in albumen and 0.15 micrograms/g in yolk for tc and 0.01 micrograms/g in albumen and 0.06 microgr ...19892912795
structure and glycosylation of lipoteichoic acids in bacillus strains.the occurrence, structure, and glycosylation of lipoteichoic acids were studied in 15 bacillus strains, including bacillus cereus (4 strains), bacillus subtilis (5 strains), bacillus licheniformis (1 strain), bacillus polymyxa (2 strains), and bacillus circulans (3 strains). whereas in the cells of b. polymyxa and b. circulans neither lipoteichoic acid nor related amphipathic polymer could be detected, the cells of other bacillus strains were shown to contain lipoteichoic acids built up of poly( ...19892914853
phospholipid requirement of ca2+-stimulated, mg2+-dependent atp hydrolysis in rat brain synaptic membranes.the phospholipid requirement for ca2+-stimulated, mg2+-dependent atp hydrolysis (ca2+/mg2+-atpase) and mg2+-stimulated atp hydrolysis (mg2+-atpase) in rat brain synaptosomal membranes was studied employing partial delipidation of the membranes with phospholipase a2 (hog pancreas), phospholipase c (bacillus cereus) and phospholipase d (cabbage). treatment with phospholipase a2 caused an increase in the activities of both ca2+/mg2+-atpase and mg2+-atpase whereas with phospholipase c treatment both ...19862946970
studies on glucosyltransferase and endogenous glucosyl acceptor in bacillus cereus ahu 1030 membranes.a glucosyltransferase, extracted from the membranes of bacillus cereus ahu 1030 with tris-hcl buffer containing 0.1% triton x-100 at ph 9.5, was separated from an endogenous glucosyl acceptor by chromatography on deae-sepharose cl-6b subsequent to chromatography on sepharose 6b. structural analysis data showed that the glucosyl acceptor was a glycerol phosphate polymer linked to beta-gentiobiosyl diglyceride. the enzyme catalyzed the transfer of glucosyl residues from udp-glucose to c-2 of the g ...19862952639
experimental bacillus cereus mastitis in cows. 19862953188
[bacillus cereus septicemia following intravenous chemotherapy with an implantable chamber]. 19872962179
[a genetic observation on a resistant variation of bacillus cereus 756 to the new antibiotic 2252]. 19872964935
antibacterial effect of lactoperoxidase and myeloperoxidase against bacillus oral periodontopathic bacterium, bacillus cereus, was inhibited both by lactoperoxidase (lp) and myeloperoxidase (mp) antimicrobial systems. with the lp-scn--h2o2 system, the growth inhibition was directly proportional to the amount of oscn- ions present. the oscn-, which is the principal oxidation product of the lp (or mp)-scn--h2o2 system at neutral ph, is a normal component of human saliva. the oxidation products of both peroxidase systems inhibited the growth of the bacteria. this inhibit ...19852984983
structural studies on the acidic polysaccharide of bacillus cereus ahu 1356 cell walls.structural studies were carried out on the acidic polymer fraction isolated from lysozyme digests of the n-acetylated cell walls of bacillus cereus ahu 1356. the acidic polymer fraction contained glucosamine, galactose, rhamnose, glycerol and phosphorus in a molar ratio of 1:1:2:1:1, together with small amounts of glycopeptide components and muramic acid 6-phosphate. the hydrogen fluoride treatment led to removal of glycerol and phosphorus from the polymer without loss of other components. resul ...19852986963
cloning and sequencing of the beta-lactamase i gene of bacillus cereus 5/b and its expression in bacillus subtilis.the beta-lactamases of bacillus cereus have attracted interest because they are secreted efficiently, because multiple enzymes are frequently present, and because their regulation has unusual features. beta-lactamase i of strain 5/b is produced constitutively at a high level, and the exoenzyme appears to be several thousand daltons larger than the corresponding product of strain 569/h. we have cloned the gene for 5/b beta-lactamase i in escherichia coli and b. subtilis and have sequenced the str ...19852991192
metabolism of exogenous nucleosides in bacillus cereus and escherichia coli. 19852997081
mechanisms of exogenous purine nucleotide utilization in bacillus cereus. 19853000698
two different parasporal inclusions are produced by bacillus thuringiensis subsp. finitimus.bacillus thuringiensis subsp. finitimus produced at least two parasporal inclusions. one inclusion was formed within the exosporium and remained with the spore after mother cell lysis. a second inclusion formed somewhat later exterior to the exosporium. each inclusion contained a major polypeptide of about 135,000 daltons with unique antigenic determinants. this subspecies contained only two plasmids, of 98 and 77 megadaltons (mda). strains cured of these plasmids produced only the free inclusio ...19863001026
catalase, superoxide dismutase, and the production of o2-sensitive mutants of bacillus coagulans.a number of facultatively anaerobic members of the genus bacillus were screened for their catalase, diaminobenzidine peroxidase, and superoxide dismutase activities. a strain of bacillus coagulans (7050) lacking peroxidatic activity and containing single catalatic and superoxide dismutase activities was selected. responses of the superoxide dismutase activity and catalase level to the partial pressure of oxygen, and fe and mn levels, as well as to aerobic and fermentative metabolism, were determ ...19853004685
effect of phospholipases on chronic opiate action in neuroblastoma x glioma ng108-15 hybrid cells.chronic treatment of neuroblastoma x glioma ng108-15 hybrid cells with opiate agonist resulted in loss of the acute opiate inhibition of adenylate cyclase activity with a concomitant increase in the enzymatic activity observable on addition of the antagonist naloxone. the role of membrane lipids in the cellular expression of these chronic opiate effects was investigated by the hydrolysis of phospholipids with various lipases. treatment with phospholipase c from clostridium welchii produced an en ...19863018158
effects of metal ions on sphingomyelinase activity of bacillus cereus.some divalent metal ions were examined for their effects on sphingomyelinase activity of bacillus cereus. the enzyme activity toward mixed micelles of sphingomyelin and triton x-100 proved to be stimulated by co2+ and mn2+, as well as by mg2+. km's for co2+ and mn2+ were 7.4 and 1.7 microm, respectively, being smaller than the km for mg2+ (38 microm). sr2+ proved to be a competitive inhibitor against mg2+, with a ki value of 1 mm. zn2+ completely abolished the enzyme activity at concentrations a ...19863019244
a spectral study of cobalt(ii)-substituted bacillus cereus phospholipase c.the coordination sphere of both the structural and catalytic zinc ions of bacillus cereus phospholipase c has been probed by substitution of cobalt(ii) for zinc and investigation of the resultant derivatives by a variety of spectroscopic techniques. the electronic absorption, circular dichroic, magnetic circular dichroic, and electron paramagnetic resonance spectra were found to be strikingly similar when cobalt(ii) was substituted into either site and are consistent with a distorted octahedral ...19863019384
phospholipase c and phosphatidylinositol phospholipase c. 19873026688
cloning and sequencing of the blaz gene encoding beta-lactamase iii, a lipoprotein of bacillus cereus 569/ has not been clear whether the membrane-bound beta-lactamase iii of bacillus cereus 569 is a separate enzyme or a modified form of the secreted beta-lactamase i. the membrane enzyme is an acyl-glyceride thioether-linked lipoprotein (j. b. k. nielsen and j. o. lampen, biochemistry 22:4652-4656, 1983) and thus is probably a separate entity. we cloned the beta-lactamase iii gene (blaz) on a 4.9-kilobase-pair clai fragment from mutant strain 569/h (constitutive for high-level production of beta-l ...19873027036
tetrameric alkaline phosphatase from human liver is converted to dimers by phosphatidylinositol phospholipase c.membrane-bound human liver alkaline phosphatase solubilized by a non-ionic detergent, nonidet p-40 (np-40), has the molecular mass of a tetramer. it can be converted to a dimeric form by treatment with a phosphatidylinositol phospholipase c (pi-plc) obtained from bacillus cereus. when human liver plasma membranes were directly treated with pi-plc, the released alkaline phosphatase was dimeric. thus, phosphatidylinositol may help maintain the tetrameric quaternary structure of alkaline phosphatas ...19873028865
the action of sphingomyelinase from bacillus cereus on atp-depleted bovine erythrocyte membranes and different lipid composition of liposomes.the presence of cholesterol or phosphatidylethanolamine in sphingomyelin liposomes enhanced 2- to 10-fold the breakdown of sphingomyelin by sphingomyelinase from bacillus cereus. on the other hand, the presence of phosphatidylcholine was either without effect or slightly stimulative at a higher molar ratio of phosphatidylcholine to sphingomyelin (3/1). in the bovine erythrocytes and their ghosts, the increase by 40-50% or the decrease by 10-23% in membranous cholesterol brought about acceleratio ...19873036001
cloning and nucleotide sequencing of genes for a second type of small, acid-soluble spore proteins of bacillus cereus, bacillus stearothermophilus, and "thermoactinomyces thalpophilus".the nucleotide sequences of the single genes coding for the b-type small, acid-soluble spore proteins (sasp) of bacillus cereus, b. stearothermophilus, and "thermoactinomyces thalpophilus" were determined, and the amino acid sequences of all b-type sasp were compared. while this type of sasp showed significant sequence conservation around the two spore protease cleavage sites, alignment of these sequences required the introduction of gaps, and even then only 19 of the residues were conserved exa ...19873036769
effects of phospholipases c from bacteria on binding of enkephalin to rat brain membranes.the effects of phospholipases c on the equilibrium constants and maximal binding capacities of tritiated [d-ala2,-d-leu5] enkephalin to rat brain membranes were investigated using phosphatidylcholine-hydrolyzing phospholipase c and sphingomyelinase c of bacillus cereus and, phosphatidylinositol-specific phospholipase c of bacillus thuringiensis. when 72% of the phosphatidylinositol in the rat brain membranes was hydrolyzed by phosphatidylinositol-specific phospholipase c, the affinity of opiate ...19873039692
[synthesis of phosphonate analogs of sphingomyelins and preparation of an affinity sorbent for sphingomyelinase purification].phosphonate analogues of 2-n-stearoyl- (i) and 2-n-(undec-10-enoyl)-sphingomyelins (ii) have been synthesised. compound (ii) was used as a starting product for preparation of a sorbent for sphingomyelinase affinity chromatography. the double bond of the unsaturated undec-10-enoyl moiety of the phosphonate analogue (ii) was oxidized, and the modified (ii) was coupled to amino-toyopearl hw-65 to give a sorbent containing 4 mumoles of ligand per milliliter of the swollen resin.19873040014
purification and properties of an extracellular collagenolytic protease produced by the human oral bacterium bacillus cereus (strain soc 67).the major collagenolytic proteinase present in the culture filtrate of bacillus cereus (strain soc 67, isolated from the human oral cavity) has been purified to homogeneity by a procedure that comprised concentration of ultrafiltered growth medium on a millipore pttk00005 membrane, precipitation with ammonium sulfate, gel permeation chromatography, chromatofocusing, fast protein liquid chromatography on an anion-exchange column, and finally fast protein liquid chromatography on a gel column. the ...19873040751
different phosphorylated forms of an insulin-sensitive glycosylphosphatidylinositol from rat hepatocytes.labeling with [3h]galactose was employed to isolate a glycosylphosphatidylinositol from rat hepatocytes which might be involved in the action of insulin. the polar head group of this glycosylphosphatidylinositol was generated by phosphodiesterase hydrolysis with a phosphatidylinositol-specific phospholipase c from bacillus cereus. by dowex ag1 x 8 chromatography the polar head group could be separated into three radioactive peaks eluting at 100 mm (peak i), 200 mm (peak ii) and 500 mm (peak iii) ...19883042467
an aspartic proteinase of erythrocyte membranes. proposed mechanism for activation and further molecular aspartic proteinase associated with human erythrocyte membranes was shown to be responsible for autodegradation of the membrane proteins at ph values below 5.0. when the membrane was treated with phospholipase c (bacillus cereus) or trypsin, and simply heated at 40 degrees c, the membrane-bound latent enzyme was activated, with this being accompanied by dissociation of the enzyme from the membrane. divalent cations such as ca2+ and mg2+ had an inhibitory effect on the dissociation of the memb ...19883060144
cellular and humoral defenses of glossina (diptera: glossinidae): reactions against bacteria, trypanosomes, and experimental implants. 19863081723
on the origin of bacterial resistance to penicillin: comparison of a beta-lactamase and a penicillin target.structural data are now available for comparing a penicillin target enzyme, the d-alanyl-d-alanine-peptidase from streptomyces r61, with a penicillin-hydrolyzing enzyme, the beta-lactamase from bacillus licheniformis 749/c. although the two enzymes have distinct catalytic properties and lack relatedness in their overall amino acid sequences except near the active-site serine, the significant similarity found by x-ray crystallography in the spatial arrangement of the elements of secondary structu ...19863082007
biosynthesis of the wall acidic polysaccharide in bacillus cereus ahu 1356.biosynthetic studies on an acidic polysaccharide, comprising galactose, rhamnose, n-acetylglucosamine and sn-glycerol 1-phosphate, were carried out with a membrane system obtained from bacillus cereus ahu 1356. incubation of the membranes with udp-[14c]gal, tdp-[14c]rha and udp-[14c]glcnac resulted in the formation of four or more labeled-sugar-linked lipids and a labeled polysaccharide. data on structural analysis of the sugar moieties released from the glycolipids, together with results of enz ...19863082626
inactivation of bacillus spores by gaseous ozone.the sporicidal activity of ozone in the gas phase was investigated. spores of six strains of bacillus species deposited on filter paper or glass fibre filter were conditioned at different relative humidities (r.h.), and then exposed to ozone ranging in concentration from 0.5 to 3.0 mg/l at different r.h. there was a lag phase in the initial stage of exposure followed by an exponential decrease in the number of survivors with time, although no lag phase was observed with one strain. inactivation ...19863082844
[post-traumatic endophthalmitis caused by bacillus cereus].a young man with severe endophthalmitis caused by b. cereus is described following a trauma with an intraocular foreign body. although this organism was isolated from the aqueous with 18 hours and specific antibiotic therapy instituted, the eye subsequently lost all function and had to be enucleated because of severe pain. an extremely rapidly developing corneal ring abscess seems to be the hallmark of an intraocular infection with b. cereus organisms.19863083151
plaster-associated bacillus cereus wound infection. a case report.plaster of paris and nonsterile cast padding have previously been implicated in wound infections. the observation of three pin site infections with bacillus cereus following pin placement and plaster fixation of an open forearm fracture led to an epidemiologic investigation. two hundred sixty-one bacterial cultures were taken from materials in the steinmann pin application and plaster fixation procedures. b. cereus with a similar antibiogram and biotype as was identified in the pin site cultures ...19863083406
germination of bacillus cereus spores induced by purine ribosides and their analogs: effects of modification of base and sugar moieties of purine nucleosides on germination-inducing activity.purine riboside and some of its analogs were tested for their ability to induce germination of bacillus cereus t spores. hypoxanthine and adenine showed no germination-inducing activity either in the present or absence of d-ribose or its phosphorylated derivatives. purine riboside and 18 analogs with modified purine base were all able to induce germination of the spores to various extents. in contrast to this, the requirement for the sugar moiety in the purine riboside appeared to be more string ...19863084054
bacillus cereus: a snake in the grass for granulocytopenic patients. 19863084672
single-stranded plasmid dna in bacillus subtilis and staphylococcus aureus.plasmid pc194 was found to exist in a double-stranded and a single-stranded dna form in bacillus subtilis and staphylococcus aureus. this single-stranded dna was found as a circular molecule of the same size as the parental monomer and corresponded to only one of the two dna strands. it represented one-third of plasmid copies. single- and double-stranded dna copies in similar proportions to the above were detected for five other s. aureus plasmids (pc221, pc223, pe194, pt127, and pt181) and one ...19863085097
[isolation and typing of bacillus cereus phages]. 19863087713
induced release of bacillus spores from sporangia by sodium sulphate.incubation of sporulating cultures of bacillus anthracis, b. cereus, b. subtilis and b. thuringiensis in 1.0 mol/l sodium sulphate markedly increased the release of free spores from sporangia. it is postulated that the release of spores is due to activation of latent autolysins which hydrolyse sporangial cell walls. sodium sulphate-induced lysis of sporangia represents a novel and highly effective method for the recovery of spores from cultures of bacillus species.19863087932
cloning and nucleotide sequencing of genes for small, acid-soluble spore proteins of bacillus cereus, bacillus stearothermophilus, and "thermoactinomyces thalpophilus".as found previously with other bacillus species, spores of b. stearothermophilus and "thermoactinomyces thalpophilus" contained significant levels of small, acid-soluble spore proteins (sasp) which were rapidly degraded during spore germination and which reacted with antibodies raised against b. megaterium sasp. genes coding for a b. stearothermophilus and a "t. thalpophilus" sasp as well as for two b. cereus sasp were cloned, their nucleotide sequences were determined, and the amino acid sequen ...19863087949
laser raman spectroscopy of lyophilized bacterial spores.laser-excited raman spectra were examined in lyophilized spores of bacillus cereus. in a comparison of the spectrum of the dormant spore with that of the germinated spore, we found several raman bands which occurred in the former but not in the latter. among these raman bands, the 1,573, 1,395, 1,017, 822, and 662 cm-1 bands were assigned to the vibrational frequencies of calcium dipicolinate (cadpa). no raman bands and peaks due to dipicolinic acid (h2dpa) were observed. this raman evidence ind ...19863088398
bacillus cereus--maine. 19863088419
purification and characterization of bacillus coagulans oligo-1,6-glucosidase.a p-nitrophenyl-alpha-d-glucopyranoside-hydrolyzing oligo-1,6-glucosidase of bacillus coagulans atcc 7050 (facultative thermophile) was purified to homogeneity. the relative molecular mass, stokes radius, sedimentation coefficient at 20 degrees c in water, molecular absorption coefficient at 280 nm and ph 6.8, and isoelectric point were estimated as 60 000, 3.29 nm, 4.8 x 10(-13) s, 1.34 x 10(5) m-1 cm-1, and 4.3, respectively. the amino-terminal amino acid was threonine. there was no common ant ...19863089779
plipastatins: new inhibitors of phospholipase a2, produced by bacillus cereus bmg302-ff67. i. taxonomy, production, isolation and preliminary characterization.plipastatins have been isolated as part of a program designed to find inhibitors of porcine pancreatic phospholipase a2. they were purified from fermentation broth of bacillus cereus bmg302-ff67 and finally separated into four fractions by reverse phase hplc. the respective fractions were designated as plipastatins a1, a2, b1 and b2. plipastatins also inhibited phospholipases c and d.19863089997
plipastatins: new inhibitors of phospholipase a2, produced by bacillus cereus bmg302-ff67. ii. structure of fatty acid residue and amino acid sequence.plipastatins, new inhibitors of phospholipase a2, were produced by a strain classified as bacillus cereus bmg302-ff67. the plipastatins are a family of acylated decapeptides which differ from each other by amino acid composition and the nature of the fatty acid side chain. the fatty acids have been shown to be 3(r)-hydroxyhexadecanoic acid (n-c16h3) and 14(s)-methyl-3(r)-hydroxyhexadecanoic acid (a-c16h3) by mass, nmr and optical rotation. amino acid sequence analysis by secondary ion mass spect ...19863089998
plipastatins: new inhibitors of phospholipase a2, produced by bacillus cereus bmg302-ff67. iii. structural elucidation of plipastatins.plipastatins are new inhibitors of phospholipase a2 produced by bacillus cereus bmg302-ff67. structures of the plipastatins have been determined by uv, mass and nmr spectrometries and chemical degradation. the carboxyl group of the c-terminal l-isoleucine of plipastatinic acid has been shown to form a lactone linkage with the hydroxyl group of l-tyrosine. the total structure of plipastatins has thus been established.19863089999
respiratory systems of the bacillus cereus mother cell and forespore.the respiratory systems of the mother cells and forespores of bacillus cereus were compared throughout the maturation stages (iii to vi) of sporulation. the results indicated that both cell compartments contain the same assortment of oxidoreductases and cytochromes. however membrane fractions from young forespores were clearly distinct from those of the mother cell, i.e., lower content of cytochrome aa3, lower cytochrome c oxidase activity, higher concentration of cytochrome o, and a lower sensi ...19863090018
a new form of coagulation factor vii in plasma.we have reported the existence of a novel form of coagulation factor vii - probably a factor vii-phospholipid complex - in plasma from pregnant women and men at risk for cardiovascular disease. we report here further observations on the presence and characteristics of this complex. some apparently healthy individuals who, on testing by standard methods, have normal levels of factor vii activity achieve such levels by means of a phospholipase c-sensitive modification of (some of) their factor vii ...19863090680
survival and growth of bacillus cereus in bread.bread doughs were artificially inoculated with spores of six bacillus cereus strains at different inoculum levels and counts of survivors in bread determined during storage at 27.5 degrees c. no b. cereus were isolated from the centre crumb of 400 g loaves when the dough contained less than 10(4) spores/g whereas with 800 g loaves survival occurred with doughs containing 5.0 x 10(3) spores/g. with all strains there was a period of at least 24 h before multiplication took place in the bread. the ...19863091561
the primary structure of bacillus cereus neutral proteinase and comparison with thermolysin and bacillus subtilis neutral proteinase.the complete amino-acid sequence of a neutral proteinase, produced by bacillus cereus, was determined by protein sequencing. the neutral proteinase consists of 317 amino-acid residues. the primary structure is 70% homologous to thermolysin, a thermostable neutral proteinase and 45% homologous to bacillus subtilis neutral proteinase. the zinc-binding site and the hydrophobic pocket of the active site are highly similar in all three proteinases. b. cereus neutral proteinase which is 20 degrees c l ...19863092843
a hospital cafeteria-related food-borne outbreak due to bacillus cereus: unique features.although bacillus cereus is a well-known cause of food-borne illness, hospital-related outbreaks of food-borne disease due to b. cereus have rarely been documented. we report a hospital employee cafeteria outbreak due to foods contaminated with b. cereus in which an outside caterer was employed to prepare the suspect meals. data were collected from 249 of 291 employees who had eaten either of the two meals. with a mean incubation period of 12.5 hours, 64% (160 of 249) of employees manifested ill ...19863093397
differential scanning calorimetry of bacteria.thermograms obtained by differential scanning calorimetry of a range of bacteria of different heat resistances were compared. equations were derived to calculate the rate at which the numbers of viable organisms in a calorimeter decline as the temperature is raised at a constant rate. vegetative bacteria scanned at 10 degrees c min-1 showed multi-peaked thermograms with four major peaks (denoted m, n, p and q) occurring in the regions 68-73, 77-84, 89-99 and 105-110 degrees c respectively. excep ...19863093634
post-traumatic bacillus cereus endophthalmitis. 19863094202
paradoxical effects of cadmium exposure on antibacterial antibody responses in two fish species: inhibition in cunners (tautogolabrus adspersus) and enhancement in striped bass (morone saxatilis).previous work in a marine fish, the cunner (tautogolabrus adspersus), showed that endocytosis of bacteria by cells in the liver and spleen was affected by 96-h exposure of the fish to cadmium at a concentration of 12 micrograms/ml; however, antibody response to sheep erythrocytes was not affected. since the latter finding was questionable because of short immunization times, and data from more than a single fish species were desirable, both the cunner and an anadromous fish, the striped bass (mo ...19863094230
an improved microbiological assay for chlortetracycline in avian plasma.bacillus cereus was used as the assay organism for the quantification of chlortetracycline (ctc) in avian plasma. antibiotic medium #8 gave significantly larger zones of inhibition than nutrient agar 1.5% when used as the assay medium (p less than or equal to 0.05). when the ctc concentration was measured in serum, citrated plasma, heparinized plasma, and oxalated plasma, no significant differences were found between the inhibition zone diameters produced on antibiotic medium #8. however, there ...19863094490
[effect of surface-active agents on the electrical properties of bacterial cells].the work was aimed at studying the effect of cationic, anionic and non-ionogenic surfactants on the frequency dependence of the electroorientation effect (eoe) and on the electrophoretic mobility (epm) of rod-like bacteria. the character of concentration dependences was found to differ for eoe and epm at a low frequency of the electric field (20 to 10(4) hz). analysis of eoe changes at a high frequency (4 x 10(5) to 3 x 10(7) hz) showed that anionic and non-ionogenic surfactants at a concentrati ...19863095615
antibodies prepared to bacillus cereus phospholipase c crossreact with a phosphatidylcholine preferring phospholipase c in mammalian cells.antibodies against bacillus cereus phospholipase c were prepared in rabbits and used to affinity purify a phosphatidylcholine-preferring phospholipase c from a human monocytic cell line. affinity chromatography resulted in an approximately 3000-fold, one-step enrichment of phospholipase c. the human enzyme had an apparent molecular mass of 40,000 daltons as determined by sds gel electrophoresis. western blotting analysis demonstrated that this protein interacted specifically with the rabbit anti ...19863096314
purification, stability and kinetic properties of highly purified adenosine deaminase from bacillus cereus ncib 8122.adenosine deaminase (adenosine aminohydrolase, ec from bacillus cereus ncib 8122 has been purified to electrophoretic homogeneity by ammonium sulfate precipitation, gel filtration through sephadex g-100, deae-sephadex a-50 chromatography and ion-exchange hplc on deae-polyol. the enzyme activity is stabilized (at temperatures from 0 degrees c to 40 degrees c) by 50 mm nh4+ or k+, while it is irreversibly lost in the absence of these or a few other monovalent cations. glycerol (24% by vol ...19863096380
biosynthesis of the wall neutral polysaccharide in bacillus cereus ahu 1356.the pathway for the biosynthesis of a cell wall polysaccharide, composed of glucosamine, mannosamine, galactosamine and glucose in a molar ratio of 4:1:1:1, was studied with a membrane system from bacillus cereus ahu 1356. in this system a glycolipid characterized as galnac(alpha 1----4)mannac(beta 1----4)glcnac-pp-undecaprenol was formed from glcnac-pp-undecaprenol by sequential transfer of n-acetylmannosamine and n-acetylgalactosamine residues from udp-mannac and udp-galnac respectively. an ad ...19863096732
numerical taxonomy of bacillus isolated from orally administered drugs.numerical taxonomy procedures were used to study 118 strains of bacillus isolated from non-sterile drugs prepared for oral administration. similarities between pairs of strains were calculated by the simple matching coefficient of sokal and michener (ssm). each strain was tested for 60 unit characters and three clusters were defined. the strains in each cluster presented a similarity level of at least 60%. cluster a comprised the strains identified as bacillus cereus (ssm = 93.13%), cluster b co ...19863096933
[rapid method for analyzing the plasmid dna of bacillus cereus]. 19863097528
heat resistance of bacterial spores correlated with protoplast dehydration, mineralization, and thermal adaptation.twenty-eight types of lysozyme-sensitive spores among seven bacillus species representative of thermophiles, mesophiles, and psychrophiles were obtained spanning a 3,000-fold range in moist-heat resistance. the resistance within species was altered by demineralization of the native spores to protonated spores and remineralization of the protonated spores to calcified spores and by thermal adaptation at maximum, optimum, and minimum sporulation temperatures. protoplast wet densities, and thereby ...19863098170
bacillus cereus panophthalmitis manifesting as an orbital cellulitis. 19873099579
changes in the coordination geometry of the active-site metal during catalysis of benzylpenicillin hydrolysis by bacillus cereus beta-lactamase ii.rapid-scanning stopped-flow spectroscopy (425-700 nm) has been used to study spectral changes in cobalt(ii)-substituted bacillus cereus beta-lactamase ii during the binding and hydrolysis of benzylpenicillin. the experiments were carried out in aqueous solution over a temperature range of 3-20 degrees c. three metallointermediates have been characterized by their visible absorption spectra. two of them have visible absorption spectra identical with the intermediates es1 and es2 previously observ ...19863099831
involvement of calcium and dipicolinic acid in the resistance of bacillus cereus bis-59 spores to u.v. and gamma radiations.the role of dipicolinic acid (dpa) in determining the resistance of bacillus cereus spores to u.v. and gamma radiation was investigated. b. cereus bis-59 spores containing varying amounts of dpa were prepared by appropriate compositional adjustments in the secondary media. compared with spores containing 6 per cent dpa (dry weight) those containing 0.8 per cent dpa were far more sensitive to u.v. radiation. similar u.v. radiation sensitivity was also found in respect of a dpa-less mutant of b. c ...19873100469
[the problem of heat activation of bacterial spores after disinfection with regard to an aerosol method of decontaminating equipment and rooms].this paper describes investigations on disinfection of germ carriers, contaminated with an alcoholic suspension of bacillus cereus or bacillus subtilis. result of disinfection is compared with that of an additional heat treatment (80 degrees c, 60 min) after disinfection. besides formalin, tegodor forte (th. goldschmidt, essen) and p 3 oxonia active (henkel kg, düsseldorf) are tested with different concentration and duration. heat activation was possible with all three disinfectants. for illumin ...19863101322
bactericidal action of carbon dioxide laser radiation in experimental dental root canals.the ability of a carbon dioxide laser to sterilize the root canal of human teeth has been investigated. three oral bacteria, streptococcus sanguis, streptococcus mutans, and actinomyces viscosus, and three other bacteria, bacillus cereus, staphylococcus aureus, and pseudomonas aeruginosa were used as experimental organisms. exposure of cells on glass slides to laser radiation showed there was little difference in the exposure required to kill these six organisms. complete recovery of bacteria fr ...19863102029
bacillus cereus endophthalmitis.the authors present a case of a young man with post-traumatic endophthalmitis caused by bacillus cereus. the clinical course was typical of the panophthalmitis caused by this toxin-producing organism: rapid onset of signs of systemic infection, a corneal ring abscess and eventual loss of the globe requiring enucleation. studies of experimental rabbit models of the infection have indicated that the most efficacious regimen consists of systemic and topical clindamycin and gentamicin. successful th ...19873102030
nucleotide sequence of the beta-lactamase i gene of bacillus cereus strains 569/h and 5/b. 19873103105
posttraumatic endophthalmitis: the emerging role of bacillus cereus infection.endophthalmitis resulting from nonsurgical penetrating trauma to the eye is a relatively uncommon infection in the united states. data are limited, but most recently published series have attributed the highest incidence of infection to gram-positive organisms, in particular staphylococcus epidermidis. fungal causes have been reported far less frequently. bacillus species are being recognized increasingly as major causes of posttraumatic ocular disease, with rates of infection often making them ...19873103191
involvement of the spore coat in germination of bacillus cereus t spores.bacillus cereus t spores were prepared on fortified nutrient agar, and the spore coat and outer membrane were extracted by 0.5% sodium dodecyl sulfate-100 mm dithiothreitol in 0.1 m sodium chloride (sds-dtt) at ph 10.5 (coat-defective spores). coat-defective spores in l-alanine plus adenosine germinated slowly and to a lesser extent than spores not treated with sds-dtt, as determined by decrease in absorbance and release of dipicolinic acid and ca2+. spores germinated in calcium dipicolinate onl ...19873103533
modification of membrane sulfhydryl groups in bacteriostatic action of nitrite.the mechanism by which nitrite inhibits outgrowing spores of bacillus cereus t was examined by using techniques developed earlier for nitrite analogs. the morphological stage of inhibition, cooperativity effects, effect of ph on inhibition, kinetics of protection against iodoacetate incorporation into membrane sulfhydryl groups, and protection against the bacteriocidal effect of carboxymethylation by iodoacetate indicate that nitrite acts as a membrane-directed sulfhydryl agent. the mechanism by ...19873103535
acetyl-coenzyme a: arylamine n-acetyltransferases in microorganisms: screening and isolation of an enzyme from bacillus cereus.the raw extracts of a series of microorganisms were screened for the presence of acetyl-coenzyme a: arylamine n-acetyltransferase (aaat) using a radioactive assay with 3h-acetyl-coenzyme a and aniline as substrates. enzyme activities were primarily detected in the soluble fractions of bacillus and nocardia species, and in some further soil organisms. only strains of bacillus cereus were able to acetylate 4-nitroaniline and 3,5-dimethyl-4-nitroaniline. the fermentation conditions for the producti ...19863103579
posttraumatic bacillus cereus endophthalmitis.we encountered a patient who developed bacillus cereus endophthalmitis following trauma. early therapy, which included intravitreal clindamycin phosphate and gentamicin sulfate, resulted in a visual acuity of 20/60. a five-year retrospective review of all cases of endophthalmitis following trauma reported at our institution revealed bacillus as the infecting organism in six (46%) of 13 culture-positive cases. the high frequency of virulent bacillus infections in the setting of trauma necessitate ...19873103589
structural and catalytic properties of l-alanine dehydrogenase from bacillus cereus.alanine dehydrogenase from bacillus cereus, a non-allosteric enzyme composed of six identical subunits, was purified to homogeneity by chromatography on blue-sepharose and sepharose 6b-cl. like other pyridine-linked dehydrogenases, alanine dehydrogenase is inhibited by cibacron blue, competitively with respect to nadh and noncompetitively with respect to pyruvate. the enzyme was inactivated by 0.1 m glycine/hcl (ph 2) and reactivated by 0.1 m phosphate (ph 8) supplemented with nad+ or nadh. the ...19873104322
endogenous bacillus cereus panophthalmitis.over the past seven years we have treated three cases of drug abusers in whom endogenous bacillus cereus endophthalmitis rapidly progressed to panophthalmitis. ocular features of infection with this organism include severe pain, chemosis, proptosis, corneal infiltration and ring abscess, subretinal exudation, retinal hemorrhages, and perivasculitis. the process becomes fulminant in an explosive manner and may be accompanied by fever and leukocytosis. ophthalmologists should be cognizant of the a ...19873105407
on the formation of a dicloxacillin-p-hydroxymercuribenzoate suicide complex mediated by beta-lactamase i from bacillus cereus.p-hydroxymercuribenzoate is a non-competitive inhibitor of beta-lactamase i from bacillus cereus and also, after preliminary preincubation, an inactivator of the enzyme. submitted to the simultaneous action of pcmb plus dicloxacillin, the enzyme completely loses its activity. extensive dialysis can restore the enzymatic activity only if preincubation had been carried out with either pcmb or dicloxacillin but not if both inhibitors had been simultaneously present. mercaptoethanol protects the enz ...19873105535
serious infections caused by bacillus species.thirty-eight patients with serious infections caused by organisms belonging to the genus bacillus are described. our experience, and that reported in the literature, indicates that, in most cases, isolated bacillus bacteremia is not a particularly serious disease. therefore, under most circumstances, empiric antibiotic therapy designed specifically for treatment of bacillus is probably not necessary. endocarditis can occur, but apparently follows bacteremia only infrequently. when these bacteria ...19873106749
factors influencing the activity of cellular alkaline phosphatase during growth and sporulation of bacillus cereus.alkaline phosphatase (ec is synthesized in media with a low phosphate concentration (0.37 mm of total and 19 microm of inorganic phosphate, respectively) already during the exponential phase of growth of bacillus cereus. the enzyme is repressed by higher phosphate concentrations (3.7 mm) during the whole growth period; during sporogenesis the enzyme activity in cells slightly increases even under these conditions. during growth the enzyme is not secreted into the medium, a minor amount ...19873108119
modulation of macrophage activity by microorganisms. 19863108148
[experimental basis of principles for creating microbiologic standards for food products for children during the 1st year of life].standard media were designed simulating the gastro-intestinal contents of infants during the first year of life. these media were used in the in vitro tests to study the viability of a number of potentially-pathogenic microorganisms. it was shown that s. aureus, e. coli o-iii, s. liquefaciens not only survived but also multiplied in the media with ph values similar to those of gastric juice in infants of the first year of life, in the presence of the mixture for child nutrition "malysh". the res ...19873109120
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