Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| evidence of mercury trapping in biofilm-eps and mer operon-based volatilization of inorganic mercury in a marine bacterium bacillus cereus bw-201b. | biofilm-forming mercury-resistant marine bacterium bacillus cereus bw-201b has been explored to evident that the bacterial biofilm-eps (exopolymers) trap inorganic mercury but subsequently release eps-bound mercury for induction of mer operon-mediated volatilization of inorganic mercury. the isolate was able to tolerate 50 ppm of mercury and forms biofilm in presence of mercury. mer operon-mediated volatilization was confirmed, and -sh was found to be the key functional group of bacterial eps re ... | 2017 | 27815566 |
| disruptions of the genes involved in lysine biosynthesis, iron acquisition, and secondary metabolisms affect virulence and fitness in metarhizium robertsii. | based on genomic analysis, polyketide synthase (pks) and nonribosomal peptide synthetase (nrps) pathways account for biosynthesis of the majority of the secondary metabolites produced by the entomopathogenic fungus metarhizium robertsii. to evaluate the contribution of these pathways to m. robertsii fitness and/or virulence, mutants deleted for mrppta, the sfp-type 4' phosphopantetheinyl transferase gene required for their activation were generated. δmrppta strains were deficient in pks and nrps ... | 2017 | 27876630 |
| characterization of three autolysins with activity against cereulide-producing bacillus isolates in food matrices. | bacillus cereus is a pathogen related with diarrhoeal or emetic food poisoning cases, of which the latter caused by the cereulide-producing isolates are more severe with several reported lethal cases. it is therefore necessary to develop an effective strategy to prevent the propagation of b. cereus in the food supply. in this study, three autolysins from the cereulide-producing b. cereus group isolates, lysis075, lysf8819.1 and lyscer057, were identified and characterized. the results showed tha ... | 2017 | 27835772 |
| bacillus cereus strain isolated from demodex folliculorum in patients with topical steroid-induced rosaceiform facial dermatitis. | the aim of the study was to identify bacillus species from the demodex folliculorum of patients with topical steroidinduced facial rosaceiform dermatitis. of the 75 patients examined, 20% had clinical spinulosis, while 18.66% had dermoscopic features of demodex: follicular plugs and tails. of the 17.33% positive patients identified upon microscopy for demodex, samples for bacterial culture were plated on trypticase soy colombia agar. identification was performed by microorganisms grown method ma ... | 2017 | 27828651 |
| multilocus sequence type profiles of bacillus cereus isolates from infant formula in china. | bacillus cereus sensu stricto is an opportunistic foodborne pathogen. the multilocus sequence type (mlst) of 74 b. cereus isolated from 513 non-random infant formula in china was analyzed. of 64 sequence types (sts) detected, 50 sts and 6 alleles were newly found in pubmlst database. all isolates except for one singleton (st-1049), were classified into 7 clonal complexes (cc) by burst (n-4), in which cc1 with core ancestral clone st-26 was the largest group including 86% isolates, and cc2, 3, 9, ... | 2017 | 27889164 |
| microbial decontamination of onion powder using microwave-powered cold plasma treatments. | the effects of microwave-integrated cold plasma (cp) treatments against spores of bacillus cereus and aspergillus brasiliensis and escherichia coli o157:h7 on onion powder were investigated. the growth of b. cereus, a. brasiliensis, and e. coli o157:h7 in the treated onion powder was assessed during storage at 4 and 25 °c, along with the physicochemical and sensory properties of the powder. onion powder inoculated with b. cereus was treated with cp using helium as a plasma-forming gas, with simu ... | 2017 | 27889137 |
| analysis of germination capacity and germinant receptor (sub)clusters of genome-sequenced bacillus cereus environmental isolates and model strains. | spore germination of 17 bacillus cereus food isolates and reference strains was evaluated using flow cytometry analysis in combination with fluorescent staining at a single-spore level. this approach allowed for rapid collection of germination data under more than 20 conditions, including heat activation of spores, germination in complex media (brain heart infusion [bhi] and tryptone soy broth [tsb]), and exposure to saturating concentrations of single amino acids and the combination of alanine ... | 2017 | 27881417 |
| effects of bacillus cereus endospores on free-living protist growth. | we studied the predator-prey interactions between heterotrophic protists and endospores of bacillus cereus group bacteria, in order to gain insight on survival and dispersal of b. cereus endospores in the environment. it has been hypothesised that the spore stage protects against digestion by predating protists. therefore, experiments were carried out to investigate the impact of b. cereus endospores and vegetative cells, as the only food source, on individual amoeboid, flagellated and ciliated ... | 2017 | 27928597 |
| antibacterial, antioxidant and anti-proliferative properties and zinc content of five south portugal herbs. | crataegus monogyna l. (rosaceae) (cm), equisetum telmateia l. (equisataceae) (et), geranium purpureum vil. (geraniaceae) (gp), mentha suaveolens ehrh. (lamiaceae) (ms), and lavandula stoechas l. spp. luisieri (lamiaceae) (ls) are all medicinal. | 2017 | 27925492 |
| one-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic atp regeneration system. | in vitro cascade catalysis using enzyme-based system is becoming a promising biomanufacturing platform for biofuels and biochemicals production. glutathione is a pivotal non-protein thiol compound and widely applied in food and pharmaceutical industries. in this study, glutathione was synthesized by a bifunctional glutathione synthetase together with a thermophilic atp regeneration system through a two-enzyme cascade in vitro. four bifunctional glutathione synthetases from streptococcus sanguini ... | 2017 | 27919691 |
| termitarium-inhabiting bacillus spp. enhanced plant growth and bioactive component in turmeric (curcuma longa l.). | curcumin (diferuloyl methane) is the main bioactive component of turmeric (curcuma longa l.) having remarkable multipotent medicinal and therapeutic applications. two bacilli isolated from termitarium soil and identified as bacillus endophyticus tsh42 and bacillus cereus tsh77 were used for bacterization of rhizome for raising c. longa ver. suguna for growth and enhancement. both the strains showed remarkable pgp activities and also chemotactic in nature with high chemotactic index. turmeric pla ... | 2017 | 27913879 |
| screening of mercury-resistant and indole-3-acetic acid producing bacterial-consortium for growth promotion of cicer arietinum l. | mercury resistant (hg(r) ) bacteria were screened from industrial effluents and effluents-polluted rhizosphere soils near to districts kasur and sheikhupura, pakistan. out of 60 isolates, three bacterial strains, bacillus sp. az-1, bacillus cereus az-2, and enterobacter cloacae az-3 showed hg-resistance as 20 μg ml(-1) of hgcl2 and indole-3-acetic acid (iaa) production as 8-38 μg ml(-1) . biochemical and molecular characterization of selected bacteria was confirmed by 16s ribotyping. mercury res ... | 2017 | 27911010 |
| hygienic characteristics and microbiological hazard identification in horse and donkey raw milk. | today the interest toward horse (equus caballus) and donkey (equus asinus) milk for human consumption is receiving a renewed attention because of its particular composition, hypoallergenicity, and nutraceutical properties. the realistic perspective of global use of this aliment in balanced diets, especially for infancy and geriatrics, poses the need for a more in depth knowledge on milk hygiene and on the health status of dairy animals, as a prerequisite of consumers' safety. the aim of this pap ... | 2017 | 27033528 |
| investigation on antimicrobial agents of the terrestrial streptomyces sp. bcc71188. | the terrestrial actinomycete strain bcc71188 was identified as streptomyces by its morphology (having spiral chain spore on the aerial mycelium), chemotaxonomy (containing ll-diaminopimelic acid in the cell wall), and 16s rrna gene sequence analysis [showing high similarity values compared with streptomyces samsunensis m1463(t) (99.85 %) and streptomyces malaysiensis nbrc 16446(t) (99.40 %)]. the crude extract exhibited antimalarial against plasmodium falciparum (ic50 0.19 μg/ml), anti-tb agains ... | 2017 | 27554496 |
| microbiological isolates and antibiotic susceptibilities: a 10-year review of culture-proven endophthalmitis cases. | to review the microbiological spectrum and antibiotic sensitivities of the pathogens that cause culture-proven endophthalmitis and to understand the status and trends of antibiotic susceptibility at a public hospital over a 10-year period. | 2017 | 27347700 |
| an in silico approach to understand the structure-function properties of a serine protease (bacifrinase) from bacillus cereus and experimental evidence to support the interaction of bacifrinase with fibrinogen and thrombin. | microbial fibrinogenolytic serine proteases find therapeutic applications in the treatment of thrombosis- and hyperfibrinogenemia-associated disorders. however, analysis of structure-function properties of an enzyme is utmost important before its commercial application. in this study, an attempt has been made to understand the structure of a fibrinogenolytic protease enzyme, "bacifrinase" from bacillus cereus strain ab01. from the molecular dynamics trajectory analysis, the modelled three-dimens ... | 2017 | 26919276 |
| fermentation of sarshir (kaymak) by lactic acid bacteria: antibacterial activity, antioxidant properties, lipid and protein oxidation and fatty acid profile. | the antioxidant and antibacterial activity of fermented sarshir (traditional dairy food), with three probiotic lactobacillus plantarum strains (lp3, af1, and lu5) were investigated. the oxidative stability and lipid profile of non-fermented and fermented sarshir were compared, as well as radical scavenging activity, peroxide, anisidine, and carbonyl values (pv, anv, and cv, respectively). | 2017 | 28349539 |
| [bacillus cereus endocarditis and a probable cutaneous gateway]. | bacillus cereus is a ubiquitous telluric organism. b. cereus endocarditis is a rare condition seen mostly in prosthetic heart valves and among intravenous drug users. we report a new case of a patient without risk factors and with a good clinical outcome not requiring valve replacement. | 2017 | 28341191 |
| biosorption of heavy metals from aqueous solution by bacteria isolated from contaminated soil. | the study was carried out to analyze the heavy metals biosorption potential of bacteria isolated from electroplating industrial effluents contaminated soil. bacterial isolates were screened for their multi-metal biosorption potential against copper, nickel, lead and chromium. bacterial isolate cu4a showed maximum uptake of copper, nickel, lead and chromium in aqueous solution with biosorption efficiency as 87.16 %, 79.62%, 84.92% and 68.12%, respectively. the bacterial strain cu4a was identified ... | 2017 | 28337961 |
| the role of pili in bacillus cereus intraocular infection. | bacterial endophthalmitis is a potentially blinding intraocular infection. the bacterium bacillus cereus causes a devastating form of this disease which progresses rapidly, resulting in significant inflammation and loss of vision within a few days. the outer surface of b. cereus incites the intraocular inflammatory response, likely through interactions with innate immune receptors such as tlrs. this study analyzed the role of b. cereus pili, adhesion appendages located on the bacterial surface, ... | 2017 | 28336259 |
| cytotoxicity, bactericidal, and antioxidant activity of sodium alginate hydrosols treated with direct electric current. | the aim of the study was to investigate the effect of using direct electric current (dc) of 0, 200, and 400 ma for five minutes on the physiochemical properties, cytotoxicity, antibacterial, and antioxidant activity of sodium alginate hydrosols with different sodium chloride concentrations. the ph, oxidation-reduction potential (orp), electrical conductivity (ec), and available chlorine concentration (acc) were measured. the effect of sodium alginate hydrosols treated with dc on staphylococcus a ... | 2017 | 28327520 |
| cellulase-assisted extraction of polysaccharides from malva sylvestris: process optimization and potential functionalities. | enzyme-assisted extraction process of the water-soluble malva sylvestris polysaccharides (msps) was optimized using response surface methodology (rsm). the highest yield (10.40%) of msps was achieved at 5.64% cellulase, 55.65°c temperature, 3.4h time, and 5.22 ph. three homogeneous polysaccharide fractions (msp-1, msp-2, msp-3) were purified by deae-cellulose and sephadex g-100 chromatography, which were composed of galactose, glucuronic acid, arabinose, rhamnose and mannose in different molar r ... | 2017 | 28327424 |
| identification of the lyso-form n-acyl intramolecular transferase in low-gc firmicutes. | bacterial lipoproteins are embedded in the cell membrane of both gram-positive and gram-negative bacteria where they serve numerous functions central to cell envelope physiology. lipoproteins are tethered to the membrane by an n-acyl-s-(mono/di)-acyl-glyceryl-cysteine anchor that is variably acylated depending on genera. in several low-gc gram-positive firmicutes, a monoacyl-glyceryl-cysteine with an n-terminal fatty acid (known as the lyso form) has been reported, though how it is formed is unk ... | 2017 | 28320885 |
| biofilm inhibition formation of clinical strains of pseudomonas aeruginosa mutans, photocatalytic activity of azo dye and gc-ms analysis of leaves of lagerstroemia speciosa. | the investigation was conducted to analyse the bioactive compounds from the leaf extracts of l. speciosa by gc-ms. the extracts were screened for antibacterial and antibiofilm activities against potential clinical strains. the bioactive compounds from the leaves of l. speciosa were extracted by soxhlet continuous extraction method and their chemical composition was analysed by gas chromatography-mass spectroscopy (gc-ms). the antibacterial activity was evaluated against clinical strain like stap ... | 2017 | 28319869 |
| structure and function of the bacterial communities during rhizoremediation of hexachlorobenzene in constructed wetlands. | vertical flow constructed wetlands (vf cws) are considered to be effective for treating organic pollutants. the rhizosphere of macrophytes such as phragmites sp., typha sp. serves as an active and dynamic zone for the microbial degradation of organic pollutants. however, it is still not clear how soil bacterial communities respond to macrophytes and pollutants during the process. for this purpose, the seedlings of phragmites australis and typha angustifolia were planted respectively in the vf cw ... | 2017 | 28316049 |
| development of a liquid chromatography-mass spectrometry based enzyme activity assay for phosphatidylcholine-specific phospholipase c. | phosphatidylcholine (pc)-specific phospholipase c (pc-plc) hydrolyzes pc to generate the second messenger 1,2-diacylglycerol (dg) and phosphocholine. pc-plc plays pivotal roles in inflammation, carcinogenesis, tumor progression, atherogenesis, and subarachnoid hemorrhage. although the activity of pc-plc in mammalian tissues was discovered approximately 40 years ago, neither the protein nor its gene has been identified. in the present study, we developed a non-radioactive enzyme activity assay fo ... | 2017 | 28315318 |
| exploiting fruit byproducts for eco-friendly nanosynthesis: citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor c6 cells. | process byproducts from the fruit industry may represent a cheap and reliable source of green reducing agents to be used in current bio-nanosynthesis. this study reports the use of orange (citrus × clementina) peel aqueous extract (ope) for one-pot green synthesis of silver nanoparticles (agnps) with high effectiveness against various microbial pathogens as well as rat glial tumor c6 cells. the effects of various operational parameters on the synthesis of agnps were systematically investigated. ... | 2017 | 28303540 |
| in vitro antibacterial, antifungal and antioxidant activities of eucalyptus spp. leaf extracts related to phenolic composition. | the crude methanolic extracts from leaves of eucalyptus camaldulensis l., e. camaldulensis var obtusa and e. gomphocephala grown in egypt were investigated to explore their chemical composition as well as their antibacterial, antifungal and antioxidant activities. major phenolics found were ellagic acid, quercetin 3-o-rhamnoside, quercetin 3-o-b-d-glucuronide, caffeic acid and chlorogenic acid. the antioxidant activities were examined by the 2,2'-diphenypicrylhydrazyl (dpph) and β-carotene-linol ... | 2017 | 28299978 |
| chemical composition and biological properties of satureja avromanica maroofi. | satureja avromanica is an indigenous plant which is frequently used as a spice in avraman-kurdistan region of iran. the present study aimed to investigate the chemical composition, antimicrobial and antioxidant properties of the s. avromanica. in addition, rosmarinic acid and total phenolic content of s. avromanica was assessed by spectrophotometric method and hptlc. the essential oil and methanolic extract were isolated by hydrodistillation and maceration methods, respectively. a total of 32 co ... | 2017 | 28298686 |
| induced systemic resistance against botrytis cinerea by bacillus cereus ar156 through a ja/et- and npr1-dependent signaling pathway and activates pamp-triggered immunity in arabidopsis. | induced resistance response is a potent and cost effective plant defense against pathogen attack. the effectiveness and underlying mechanisms of the suppressive ability by bacillus cereus ar156 to pseudomonas syringae pv. tomato dc3000 (pst dc3000) in arabidopsis has been investigated previously; however, the strength of induced systemic resistance (isr) activity against botrytis cinerea remains unknown. here, we show that root-drench application of ar156 significantly reduces disease incidence ... | 2017 | 28293243 |
| prevalence, virulence factor genes and antibiotic resistance of bacillus cereus sensu lato isolated from dairy farms and traditional dairy products. | b. cereus are of particular interest in food safety and public health because of their capacity to cause food spoilage and disease through the production of various toxins. the aim of this study was to determine the prevalence, virulence factor genes and antibiotic resistance profile of b. cereus sensu lato isolated from cattle grazing soils and dairy products in ghana. a total of 114 samples made up of 25 soil collected from cattle grazing farm land, 30 raw milk, 28 nunu (yoghurt-like product) ... | 2017 | 28288581 |
| genetic and genomic diversity of nheabc locus from bacillus strains. | non-hemolytic enterotoxin (nhe), a tri-partite, proteinaceous toxin encoded by contiguous nhea, nheb and nhec genes of bacillus cereus sensu lato (b. cereus s.l.), is considered to be associated with the foodborne diarrheic syndrome. however, b. cereus s.l. includes a number of closely related strains, and the occurrence of nhe among them, and other members of bacillus is unclear. consequently, we aimed to determine the distribution and evolution of nhe within bacillus by confirming the presence ... | 2017 | 28283680 |
| complete genome sequences of bacillus phages dirtybetty and kida. | we report here the genome sequences of two bacteriophages of the bacillus cereus group, dirtybetty and kida. these bacteriophages are double-stranded dna-containing myoviridae isolated from soil samples using bacillus thuringiensis subsp. kurstaki as their host bacteria. | 2017 | 28280018 |
| antibacterial activity and synergistic antibacterial potential of biosynthesized silver nanoparticles against foodborne pathogenic bacteria along with its anticandidal and antioxidant effects. | silver nanoparticles plays a vital role in the development of new antimicrobial substances against a number of pathogenic microorganisms. these nanoparticles due to their smaller size could be very effective as they can improve the antibacterial activity through lysis of bacterial cell wall. green synthesis of metal nanoparticles using various plants and plant products has recently been successfully accomplished. however, few studies have investigated the use of industrial waste materials in nan ... | 2017 | 28261161 |
| trimethylamine removal by plant capsule of sansevieria kirkii in combination with bacillus cereus en1. | trimethylamine (tma) contamination produces a strong "fishy" odor and can cause pathological changes in humans. by screening native microorganisms from sansevieria kirkii exposed to 100 ppm tma, it was shown that endophytic bacteria number 1 (en1) and number 2 (en2) have a higher tma tolerance and removal capacity than other bacteria species in a closed system. in addition, en1 and en2 demonstrated the ability to produce high quantities of indole-3-acetic acid (iaa) and use 1-aminocyclopropane-1 ... | 2017 | 28258430 |
| the bacterial sequential markov coalescent. | bacteria can exchange and acquire new genetic material from other organisms directly and via the environment. this process, known as bacterial recombination, has a strong impact on the evolution of bacteria, for example leading to the spread of antibiotic resistance across clades and species, and to the avoidance of clonal interference. recombination hinders phylogenetic and transmission inference because it creates patterns of substitutions (homoplasies) inconsistent with the hypothesis of a si ... | 2017 | 28258183 |
| microbiological quality of raw milk attributable to prolonged refrigeration conditions. | refrigerated storage of raw milk is a prerequisite in dairy industry. however, temperature abused conditions in the farming and processing environments can significantly affect the microbiological quality of raw milk. thus, the present study investigated the effect of different refrigeration conditions such as 2, 4, 6, 8, 10 and 12 °c on microbiological quality of raw milk from three different dairy farms with significantly different initial microbial counts. the bacterial counts (bc), protease ... | 2017 | 28252354 |
| impact of endophytic colonization patterns on zamioculcas zamiifolia stress response and in regulating ros, tryptophan and iaa levels under airborne formaldehyde and formaldehyde-contaminated soil conditions. | deeper understanding of plant-endophyte interactions under abiotic stress would provide new insights into phytoprotection and phytoremediation enhancement. many studies have investigated the positive role of plant-endophyte interactions in providing protection to the plant against pollutant stress through auxin (indole-3-acetic acid (iaa)) production. however, little is known about the impact of endophytic colonization patterns on plant stress response in relation to reactive oxygen species (ros ... | 2017 | 28246037 |
| should we care about transmission of infectious bacterial agents through serum handling? | immunological assays are valuable diagnostic tools to detect infections due to most of bacterial microorganisms. however, the question is how much safe are the common serum samples used as sources of antibodies in these assays? here, we experimentally followed the issue. 10-fold serial dilutions of two gram-positive, staphylococcus aureus and bacillus cereus, and one gram-negative bacteria, escherichia coli, were prepared and spiked into freshly taken blood samples of human and four animal speci ... | 2017 | 28242424 |
| the antibacterial activity of extracts of nine plant species with good activity against escherichia coli against five other bacteria and cytotoxicity of extracts. | the development of antibiotic resistant bacteria stems from a number of factors, including inappropriate use of antibiotics in human and animal health and their prolonged use as growth promoters at sub-clinical doses in poultry and livestock production. we were interested in investigating plants that could be useful in protecting humans or animals against diarrhoea. we decided to work on extracts of nine plant species with good activity against escherichia coli based on earlier work in the phyto ... | 2017 | 28241818 |
| draft genome sequences of enterotoxigenic bacillus cereus strains obtained from powdered infant formula. | we introduce the draft genome sequences of five enterotoxigenic bacillus cereus strains: bc 12, bc 67, bc 111, bc 112, and bc 113, which were obtained from powdered infant formula. the genome sizes of the strains ranged from 5.5 to 5.8 mb, and the g+c contents were ~35.2%. | 2017 | 28232440 |
| synthetic investigation, physicochemical characterization and antibacterial evaluation of ternary bi(iii) systems with hydroxycarboxylic acid and aromatic chelator substrates. | due to its physical and chemical properties, bismuth (bi(iii)) is widely used in the treatment of several gastrointestinal and skin diseases, and infections caused by bacteria. herein, its known antimicrobial potential was taken into consideration in the synthesis of two new hybrid ternary materials of bi(iii) with the physiological α-hydroxycarboxylic glycolic acid and 1,10-phenanthroline (phen), [bi2(c2h2o3)2(c2h3o3)(no3)]n(.) nh2o (1) and [bi(c12h8n2)(no3)4](c10h8n4) (2), aiming at improving ... | 2017 | 28231455 |
| spectroscopy analysis and molecular dynamics studies on the binding of penicillin v and sulbactam to beta-lactamase ii from bacillus cereus. | the molecular recognition and interaction of beta-lactamase ii from bacillus cereus (bc ii) with penicillin v (pv) and sulbactam (sul) especially conformational changes of bc ii in the binding process were studied through spectroscopy analysis in combination with molecular dynamics (md) simulation. the results show that in the binding process, a new coordination bond is observed between the zn2 of bc ii and the carboxyl-o of pv or sul by replacing his204. electrostatic interaction between zn2 an ... | 2017 | 28219797 |
| microbiological analysis, antimicrobial activity, and heavy-metals content of jordanian ma'in hot-springs water. | ma'in hot springs are known as sites of balneotherapy. however, little is known about their microbiology and chemistry. in this study, we aim at evaluating the antimicrobial activity of ma'in hot-springs water (mhsw), studying its microbiology, and determining its physicochemical properties including the heavy metals content. therefore, water samples were collected from ma'in hot springs and tested for antimicrobial activity using agar diffusion method. water was then cultivated on nutrient agar ... | 2017 | 28214187 |
| heat-resistance of psychrotolerant bacillus cereus vegetative cells. | spores of psychrotolerant strains of the foodborne pathogen bacillus cereus can multiply during storage of cooked or pasteurized, refrigerated foods and can represent a risk if these cells are not eliminated during reheating of food product before consumption. we determined the heat-resistance of psychrotolerant b. cereus vegetative cells at different heating temperatures in laboratory medium and compared it with that of thermotolerant b. cereus vegetative cells. the z values, based on times for ... | 2017 | 28213026 |
| kinneretia thg-sqi4 mediated biosynthesis of silver nanoparticles and its antimicrobial efficacy. | simple, facile, effective approach for the biosynthesis of silver nanoparticles using kinneretia species and its antimicrobial activity against human pathogens has been demonstrated in this study. kinneretia thg-sqi4 has been isolated from soil sample collected from shangqui, china. the synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry (uv-vis), field emission transmission electron microscopy (fe-tem), energy dispersive x-ray spectroscopy (edx), elemental mapp ... | 2017 | 28211298 |
| improvement of polymyxin-egg yolk-mannitol-bromothymol blue agar for the enumeration and isolation of bacillus cereus in various foods. | a modified polymyxin-egg yolk-mannitol-bromothymol blue agar (mpemba) was developed by supplementing polymyxin-egg yolk-mannitol-bromothymol blue agar (pemba) with trimethoprim to improve the selectivity for and recoverability of bacillus cereus from naturally and artificially contaminated food samples. the number of b. cereus in mpemba was significantly higher than in pemba, indicating better recoverability (p < 0.05) in red pepper powder (pemba 0.80 ± 0.22 log cfu/g versus mpemba 1.95 ± 0.17 l ... | 2017 | 28207304 |
| isolation and characterization of bacillus cereus bacteriophages from foods and soil. | the aim of this study was to isolate and characterize bacillus cereus bacteriophages of various origins. twenty-seven bacteriophages against b. cereus were isolated from various korean traditional fermented foods and soils. plaque size, transmission electron microscopy, virulence profile, and in vitro lytic activity of bacteriophage isolates were examined. transmission electron microscopy confirmed b. cereus bacteriophages belonging to the family siphoviridae. among b. cereus bacteriophages with ... | 2017 | 28205130 |
| compensatory evolution of intrinsic transcription terminators in bacillus cereus. | 2017 | 28201729 | |
| antibiogram of bacteria isolated from automated teller machines in hamadan, west iran. | aim: bacteria are ubiquitous in the environment. in keeping with the continued expansion of urbanization and the growing population, an increasing number of people use automated banking, i.e. automated teller machines (atms). the aim of this study was to investigate the bacterial contamination and its antibiotic sensitivity on computer keyboards located at atms in hamadan province, iran. method: out of 360 atms at four locations in hamadan, 96 were randomly selected for this study. the antibioti ... | 2017 | 28197394 |
| spoilage of microfiltered and pasteurized extended shelf life milk is mainly induced by psychrotolerant spore-forming bacteria that often originate from recontamination. | premature spoilage and varying product quality due to microbial contamination still constitute major problems in the production of microfiltered and pasteurized extended shelf life (esl) milk. spoilage-associated bacteria may enter the product either as part of the raw milk microbiota or as recontaminants in the dairy plant. to identify spoilage-inducing bacteria and their routes of entry, we analyzed end products for their predominant microbiota as well as the prevalence and biodiversity of psy ... | 2017 | 28197147 |
| design, synthesis, and antibacterial activities of novel heterocyclic arylsulphonamide derivatives. | design, synthesis, and antibacterial activities of a series of arylsulphonamide derivatives as probable peptide deformylase (pdf) inhibitors have been discussed. compounds have been designed following lipinski's rule and after docking into the active site of pdf protein (pdb code: 1g2a) synthesized later on. furthermore, to assess their antibacterial activity, screening of the compound was done in vitro conditions against gram-positive and gram-negative bacterial strains. in silico, studies reve ... | 2017 | 28194576 |
| substance p and calcitonin gene-related peptide: key regulators of cutaneous microbiota homeostasis. | neurohormones diffuse in sweat and epidermis leading skin bacterial microflora to be largely exposed to these host factors. bacteria can sense a multitude of neurohormones, but their role in skin homeostasis was only investigated recently. the first study focused on substance p (sp), a neuropeptide produced in abundance by skin nerve terminals. sp is without effect on the growth of gram-positive (bacillus cereus, staphylococcus aureus, and staphylococcus epidermidis) and gram-negative (pseudomon ... | 2017 | 28194136 |
| emergence of multi drug resistance among soil bacteria exposing to insecticides. | impacts of pesticide exposure on the soil microbial flora and cross resistance to antibiotics have not been well documented. development of antibiotic resistance is a common issue among soil bacteria which are exposing to pesticides continuously at sub-lethal concentration. the present study was focused to evaluate the correlation between pesticide exposures and evolution of multi drug resistance among isolates collected from soil applied with insecticides. twenty five insecticide (monochrotopho ... | 2017 | 28192223 |
| an assessment of the microbiological quality of liver-based pâté in england 2012-13: comparison of samples collected at retail and from catering businesses. | the purpose of this study was to investigate the microbiological quality of liver pâté. during 2012-13, a total of 870 samples, unrelated to the investigation of food-poisoning outbreaks, were collected either at retail (46%), catering (53%) or the point of manufacture (1%) and were tested using standard methods to detect salmonella spp. or campylobacter spp., and to enumerate for listeria spp., including listeria monocytogenes, clostridium perfringens, coagulase-positive staphylococci including ... | 2017 | 28190406 |
| functional expression and purification of recombinant hepcidin25 production in escherichia coli using sumo fusion technology. | hepcidin25 is a small cysteine-rich peptide hormone known as a new class of antimicrobial peptides. the purpose of the present study was to express, purify and investigate the antibacterial properties of recombinant human hepcidin25 protein production in escherichia coli. human hepcidin25 gene was optimized and fused to a small ubiquitin-related modifier (sumo) gene for higher expression. then sumo-hepcidin25 was cloned into the pet-32a (+) vector and expressed in e. coli origami. the fusion pro ... | 2017 | 28188870 |
| molecular recognition and binding of beta-lactamase ii from bacillus cereus with penicillin v and sulbactam by spectroscopic analysis in combination with docking simulation. | the molecular recognition and binding interaction of beta-lactamase ii from bacillus cereus (bc ii) with penicillin v (pv) and sulbactam (sul) at 277 k were studied by spectroscopic analysis and molecular docking. the results showed that a non-fluorescence static complex was separately formed between bc ii and two ligands, the molecular ratio of bc ii to pv or sul was both 1:1 in the binding and the binding constants were 2.00 × 10(6) and 3.98 × 10(5) (l/mol), respectively. the negative free ene ... | 2017 | 28185399 |
| enhanced extracellular production of recombinant proteins in escherichia coli by co-expression with bacillus cereus phospholipase c. | our laboratory has reported a strategy for improving the extracellular production of recombinant proteins through co-expression with thermobifida fusca cutinase, which increases membrane permeability via its phospholipid hydrolysis activity. however, the foam generated by the lysophospholipid product makes the fermentation process difficult to control in a fermentor. phospholipase c (plc) catalyzes the hydrolysis of phospholipids to produce sn1,2-diacylglycerides and organic phosphate, which do ... | 2017 | 28178978 |
| alliacane sesquiterpenoids from submerged cultures of the basidiomycete inonotus sp. bcc 22670. | nine alliacane sesquiterpenoids, inonoalliacanes a-i, were isolated from culture broth of the basidiomycete inonotus sp. bcc 22670. the structures were elucidated on the basis of nmr spectroscopic and mass spectrometry data. the absolute configuration of inonoalliacane f was determined by application of the modified mosher's method. inonoalliacane a, the most abundant sesquiterpene constituent, exhibited moderate antibacterial activity against bacillus cereus, whereas inonoalliacane b showed ant ... | 2017 | 28173949 |
| microbial interactions in the phyllosphere increase plant performance under herbivore biotic stress. | the phyllosphere supports a tremendous diversity of microbes and other organisms. however, little is known about the colonization and survival of pathogenic and beneficial bacteria alone or together in the phyllosphere across the whole plant life-cycle under herbivory, which hinders our ability to understand the role of phyllosphere bacteria on plant performance. we addressed these questions in experiments using four genetically and biogeographically diverse accessions of arabidopsis thaliana, t ... | 2017 | 28163703 |
| epidihydropinidine, the main piperidine alkaloid compound of norway spruce (picea abies) shows promising antibacterial and anti-candida activity. | this study reports for the first time promising antibacterial and antifungal effects of epidihydropinidine, the major piperidine alkaloid in the needles and bark of norway spruce, picea abies (l.) karsten. epidihydropinidine was growth inhibitory against all bacterial and fungal strains used in our investigation, showing the lowest mic value of 5.37μg/ml against pseudomonas aeruginosa, enterococcus faecalis, candida glabrata and c. albicans. epidihydropinidine was nearly three times more active ... | 2017 | 28163074 |
| draft genome sequences of 18 psychrotolerant and 2 thermotolerant strains representative of particular ecotypes in the bacillus cereus group. | bacteria from the bacillus cereus group exhibit genetic and physiological diversity through different ecotypes. here, we present the draft genome sequences of 20 bacterial strains belonging to the contrasted psychrotolerant and thermotolerant ecotypes. | 2017 | 28153905 |
| the essential oil from the fruits of the brazilian spice xylopia sericea a. st.-hil. presents expressive in-vitro antibacterial and antioxidant activity. | the aims of this study were to investigate the chemical composition and the antioxidant activity and antibacterial activity of the essential oil of xylopia sericea fruits (oxs). the fruits of this species are popularly used for medicinal purposes, and as a condiment in food preparation. | 2017 | 28134988 |
| whole-genome resequencing of bacillus cereus and expression of genes functioning in sodium chloride stress. | bacillus cereus is one of the most common opportunistic pathogens responsible for various foodborn diseases. to investigate the regulatory mechanism of b. cereus under high osmotic pressure, two b. cereus strains b25 and b26 were isolated from the industrial soy sauce residue containing high-salt concentration. resequencing was performed by illumina/solexa platform and 13,646 snps and 434 indels were identified as common variants between b25 and b26 against reference genome, followed by cog, go, ... | 2017 | 28131953 |
| draft genome sequence of flavobacterium johnsoniae ci04, an isolate from the soybean rhizosphere. | flavobacterium johnsoniae ci04 was coisolated with bacillus cereus from a root of a field-grown soybean plant in arlington, wi, and selected as a model for studying commensalism between members of the cytophaga-flavobacterium-bacteroides group and b. cereus here we report the draft genome sequence of f. johnsoniae ci04 obtained by illumina sequencing. | 2017 | 28126949 |
| hptlc fingerprint profile, in vitro antioxidant and evaluation of antimicrobial compound produced from brevibacillus brevis-egs9 against multidrug resistant staphylococcus aureus. | in the present study, in vitro antimicrobial activity of brevibacillus brevis egs9 against multi drug resistant staphylococcus aureus (mdrsa) and to investigate the antimicrobial, antioxidant activity and hptlc finger print profile of brevibacillus brevis egs9. primary screening was done using by cross streak method against multi drug resistant staphylococcus aureus. the bioactive metabolites were extracted from brevibacillus brevis egs9 using ethyl acetate extraction. ethyl acetate extract show ... | 2017 | 27939872 |
| sodium alginate and gum acacia hydrogels of zno nanoparticles show wound healing effect on fibroblast cells. | an ideal biomaterial for wound dressing applications should possess antibacterial and anti-inflammatory properties without any toxicity to the host cells while providing the maximum healing activity. zinc oxide nanoparticles (znonps) possess antimicrobial activity and enhance wound healing, but the questions regarding their safety arise before application to the biological systems. we synthesized znonps-loaded-sodium alginate-gum acacia hydrogels (saga-znonps) by cross linking hydroxyl groups of ... | 2017 | 27939272 |
| alleviation of heavy metals toxicity by the application of plant growth promoting rhizobacteria and effects on wheat grown in saline sodic field. | the aim of the study was to determine tolerance of plant growth promoting rhizobacteria (pgpr) in different concentrations of cu, cr, co, cd, ni, mn, and pb and to evaluate the pgpr-modulated bioavailability of different heavy metals in the rhizosphere soil and wheat tissues, grown in saline sodic soil. bacillus cereus and pseudomonas moraviensis were isolated from cenchrus ciliaris l. growing in the khewra salt range. seven-day-old cultures of pgpr were applied on wheat as single inoculum, co-i ... | 2017 | 27936865 |
| bacteria and hoverflies (diptera: syrphidae) in tree hollows from the iberian mediterranean forest. | saproxylic insect communities inhabiting tree hollows in mediterranean forests depend on a combination of physical characteristics and interactions occurring between community member species. despite the need to preserve these organisms, little is known about their interrelationships, in particular those relationships between saproxylic insects and microbiota occurring in these microhabitats. in tree hollows of quercus rotundifolia lamark that hold water and contain dead leaves, abundant microbi ... | 2017 | 28025224 |
| cmc stabilized nano silver synthesis, characterization and its antibacterial and synergistic effect with broad spectrum antibiotics. | in the present study silver nanoparticles were synthesized by reduction of agno3 using aqueous cmc solution, which acts as both reducing and capping agent. the formation of agno3 nanoparticles was observed visually by color change and these nanoparticles were characterized through uv-vis spectroscopy, ftir, xrd, sem, eds and afm. the ftir peaks observed to be ranging from 3300 to 605cm(-1). the afm image clearly showed the surface morphology of well dispersed nanoparticles. sem image illustrates ... | 2017 | 28024537 |
| inactivation and changes in metabolic profile of selected foodborne bacteria by 460 nm led illumination. | the objective of this study was to investigate the effect of 460 nm light-emitting diode (led) on the inactivation of foodborne bacteria. additionally, the change in the endogenous metabolic profile of led illuminated cells was analyzed to understand the bacterial response to the led illumination. six different species of bacteria (bacillus cereus, listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7, pseudomonas aeruginosa and salmonella typhimurium) were illuminated with 460 ... | 2017 | 28040158 |
| antibiotic discovery throughout the small world initiative: a molecular strategy to identify biosynthetic gene clusters involved in antagonistic activity. | the emergence of bacterial pathogens resistant to all known antibiotics is a global health crisis. adding to this problem is that major pharmaceutical companies have shifted away from antibiotic discovery due to low profitability. as a result, the pipeline of new antibiotics is essentially dry and many bacteria now resist the effects of most commonly used drugs. to address this global health concern, citizen science through the small world initiative (swi) was formed in 2012. as part of swi, stu ... | 2017 | 28110506 |
| bacillus cereus bacteraemia: comparison between haematologic and nonhaematologic patients. | bacillus cereus bacteraemia can be severe, especially among patients with haematologic malignancy. we retrospectively reviewed first episodes of true b. cereus bacteraemia (more than one positive bottle plus signs of infection) at our institution between 1997 and 2013 with the aim to compare haematologic versus nonhaematologic patients and analyse episodes with complicated outcome. among 56 episodes of positive-blood cultures for b. cereus, 21 were considered significant. median age was 54 years ... | 2017 | 28050250 |
| bioremediation of petroleum wastewater by hyper-phenol tolerant bacillus cereus: preliminary studies with laboratory-scale batch process. | petroleum wastewater samples from oil refinery and oil exploration site were treated by hyper phenol-tolerant bacillus cereus (akg1 and akg2) in laboratory-scale batch process to assess their bioremediation efficacy. quality of the treated wastewater samples were analyzed in terms of removal of chemical oxygen demand (cod), total organic carbon (toc) and ammonium nitrogen content, and improvement of biological oxygen demand (bod). adaptation of these bacteria to the toxic environment through str ... | 2017 | 28095107 |
| artp mutation and genome shuffling of abe fermentation symbiotic system for improvement of butanol production. | butanol is an ideal renewable biofuel which possesses superior fuel properties. previously, butanol-producing symbiotic system tsh06 was isolated in our lab, with microoxygen tolerance ability. to boost butanol yield for large-scale industrial production, tsh06 was used as parental strain and subjected to atmospheric and room temperature plasma (artp) and four rounds of genome shuffling (gs). artp mutant and gs strain were co-cultured with facultative anaerobic bacillus cereus tsh2 to form a sym ... | 2017 | 28074224 |
| biochemical and molecular study of carpobrotus edulis bioactive properties and their effects on dugesia sicula (turbellaria, tricladida) regeneration. | the traditional medicinal properties of carpobrotus edulis are well recognized, particularly in tunisia where it is used for wound healing. thus, in this study, biochemical and molecular properties of its leaves' bioactive aqueous-acetone extract were investigated. the total phenolic content (tpc) of the extract was estimated to be 184 ± 5 mg/100 g of fresh matter (fm). the qualitative and quantitative polyphenolic profile was determined by ultra performance liquid chromatography with diode arra ... | 2017 | 28064427 |
| terphenyl derivatives and drimane - phathalide/isoindolinones from hypoxylon fendleri bcc32408. | the genus hypoxylon, a member of the family xylariaceae, has been known to produce significant secondary metabolites in terms of chemical diversity. moreover, the compounds isolated can also be used as chemotaxonomic characters for differentiation among the two sections, which are sect. annulata and sect. hypoxylon. in our continuing chemical screening programme for novel compounds, the crude extracts of h. fendleri bcc32408 gave significant chemical profiles in hplc analyses. thus, the chemical ... | 2017 | 28384525 |
| characterization of plasma membrane ceramides by super-resolution microscopy. | the sphingolipid ceramide regulates cellular processes such as differentiation, proliferation, growth arrest, and apoptosis. ceramide-rich membrane areas promote structural changes within the plasma membrane that segregate membrane receptors and affect membrane curvature and vesicle formation, fusion, and trafficking. ceramides were labeled by immunocytochemistry to visualize their distribution on the plasma membrane of different cells with virtually molecular resolution by direct stochastic opt ... | 2017 | 28379629 |
| a new anthraquinone and a new naphthoquinone from the whole plant of spermacoce latifolia. | a phytochemical study on the whole plant of spermacoce latifolia led to the isolation of a new anthraquinone, 1,2,6-trihydroxy-5-methoxy-9,10-anthraquinone (1), and a new naphthoquinone, (2r)-6-hydroxy-7-methoxy-dehydroiso-α-lapachone (2), together with three known anthraquinones (3-5). their structures were established on the basis of detailed spectroscopic analysis, including one- and two-dimensional nmr, esi-ms, and hr-esi-ms techniques. all the compounds were isolated from s. latifolia for t ... | 2017 | 28357881 |
| n-heterocyclic choline analogues based on 1,2,3,4-tetrahydro(iso)quinoline scaffold with anticancer and anti-infective dual action. | pharmacological effects of biologically active "small molecules" can be improved by their targeted modification, which affects drug delivery and interaction with tumor cells and microorganisms. we aimed to evaluate anticancer and antimicrobial activity of lipid-like choline derivatives modified via simultaneous introduction of tetrahydro(iso)quinoline based pharmacophore system at nitrogen atom and long chain alkyl substituent at oxygen atom. | 2017 | 28364698 |
| synthesis and biological evaluation of a new series of benzimidazole derivatives as antimicrobial, antiquorum-sensing and antitumor agents. | new benzimidazole derivatives were synthesized and assessed for antimicrobial efficacy toward escherichia coli, bacillus cereus, staphylococcus aureus, candida albicans and aspergillus fumigatus 293. results indicated that compounds 3c and 3n have promising activity toward s. aureus, whereas 3i exhibited remarkable efficacy toward b. cereus. moreover, compound 3c was proved to be the most active antifungal analog toward c. albicans. on the other hand, 3n displayed the highest activity against a. ... | 2017 | 28334654 |
| genotypic heterogeneity of emetic toxin producing bacillus cereus isolates from china. | emetic toxin-producing bacillus cereus (emetic b. cereus) is the third member of b. cereus group whose toxins are encoded by megaplasmids, beside anthrax and insecticidal toxins of b. anthracis and b. thuringiensis, respectively. a total of 18 emetic isolates collected from food poisoning events, clinical and non-random food samples in zhejiang province of china, were analyzed by plasmid screening, pulse field gel electrophoresis, multilocus sequence typing, and toxic gene identification to inve ... | 2017 | 27744366 |
| a four-gene operon in bacillus cereus produces two rare spore-decorating sugars. | bacterial glycan structures on cell surfaces are critical for cell-cell recognition and adhesion and in host-pathogen interactions. accordingly, unraveling the sugar composition of bacterial cell surfaces can shed light on bacterial growth and pathogenesis. here, we found that two rare sugars with a 3-c-methyl-6-deoxy-hexose structure were linked to spore glycan in bacillus cereus atcc 14579 and atcc 10876. moreover, we identified a four-gene operon in b. cereus atcc 14579 that encodes proteins ... | 2017 | 28298443 |
| molecular tiling on the surface of a bacterial spore - the exosporium of the bacillus anthracis/cereus/thuringiensis group. | bacteria of the genera bacillus and clostridium form highly resistant spores, which in the case of some pathogens act as the infectious agents. an exosporium forms the outermost layer of some spores; it plays roles in protection, adhesion, dissemination, host targeting in pathogens and germination control. the exosporium of the bacillus cereus group, including the anthrax pathogen, contains a 2d-crystalline basal layer, overlaid by a hairy nap. bcla and related proteins form the hairy nap, and r ... | 2017 | 28214340 |
| evaluating bionanoparticle infused fungal metabolites as a novel antimicrobial agent. | therapeutic properties of fungal metabolites and silver nanoparticles have been well documented. while fungal metabolites have been used for centuries as medicinal drugs, potential of biogenic silver nanoparticles has recently received attention. we have evaluated the antimicrobial potential of aspergillus terreus crude extract, silver nanoparticles and an amalgamation of both against four pathogenic bacterial strains. antimicrobial activity of the following was evaluated - a. terreus extract, b ... | 2017 | 27429931 |
| bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides. | several products of industrial interest are produced by bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. this study aimed to investigate the potential of two bacterial isolates (p5 and c3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. phylogenetic analyses showed close relation of isolates p5 and c3 with bacillus amyloliquefaciens and bacillus thuringie ... | 2017 | 28197131 |
| dynamics of bacterial class bacilli in the deepest valley lake of kashmir-the manasbal lake. | in recognition of the importance of bacteria as ecological indicators of the aquatic systems a comprehensive and systematic analysis was carried out on manasbal lake, the deepest spring fed valley lake of kashmir. the main objective envisaged was to analyze bacterial community composition (bcc) and for this purpose systematic and regular sampling of waters from ten different sampling stations, predetermined in the lake according to differences in degree of human interference and also as zones of ... | 2017 | 28087491 |