Publications

molecular characterization and risk assessment of bacillus cereus sensu lato isolated from ultrahigh-temperature and pasteurized milk marketed in rio de janeiro, brazil.	the presence of bacillus cereus in milk is a major concern in the dairy industry. in this study 27 bacillus cereus sensu lato isolates from pasteurized and ultrahigh-temperature (uht) milk (24 whole uht and 4 pasteurized samples) collected at supermarket chains in rio de janeiro, brazil, were evaluated to assess the potential risk for food poisoning. toxigenic and virulence profiles were defined by gene-specific pcr. affiliation to phylogenetic groups was assigned by panc sequencing. microbiolog ...	2017	28556679
a study to assess the numbers and prevalence of bacillus cereus and its toxins in pasteurized fluid milk.	bacillus cereus is a pathogenic adulterant of raw milk and can persist as spores and grow in pasteurized milk. the objective of this study was to determine the prevalence of b. cereus and its enterotoxins in pasteurized milk at its best-before date when stored at 4, 7, and 10°c. more than 5.5% of moderately temperature-abused products (stored at 7°c) were found to contain >10(5) cfu/ml b. cereus , and about 4% of them contained enterotoxins at a level that may result in foodborne illness; in add ...	2017	28561637
molecular and functional characterization of myxobacteria isolated from soil in india.	this study reports the isolation of myxobacteria from soil collected from plains in north india. based on the morphology and 16s rdna sequence, the isolated myxobacteria were identified as corallococcus sp., pyxidicoccus sp., myxococcus sp., cystobacter sp. and archangium sp. the myxobacteria were functionally characterized to assess their ability to produce antibacterial and anticancer metabolites. the isolates were found to be functionally versatile as they produced extracellular bioactive mol ...	2017	28567623
recombinant protein expression, crystallization, and biophysical studies of a bacillus-conserved nucleotide pyrophosphorylase, bcmazg.	to overcome safety restrictions and regulations when studying genes and proteins from true pathogens, their homologues can be studied. bacillus anthracis is an obligate pathogen that causes fatal inhalational anthrax. bacillus cereus is considered a useful model for studying b. anthracis due to its close evolutionary relationship. the gene cluster ba1554 - ba1558 of b. anthracis is highly conserved with the bc1531- bc1535 cluster in b. cereus, as well as with the bt1364-bt1368 cluster in bacillu ...	2017	28570540
bacterial contaminants from frozen puff pastry production process and their growth inhibition by antimicrobial substances from lactic acid bacteria.	seventy-five bacterial contaminants which still persisted to cleaning system from three puff pastry production lines (dough forming, layer and filling forming, and shock freezing) were identified using 16s rdna as seven genera of bacillus, corynebacterium, dermacoccus, enterobacter, klebsiella, pseudomonas, and staphylococcus with detection frequencies of 24.00, 2.66, 1.33, 37.33, 1.33, 2.66, and 30.66, respectively. seventeen species were discovered while only 11 species bacillus cereus, b. sub ...	2017	28572930
phosphate crosslinked pectin based dual responsive hydrogel networks and nanocomposites: development, swelling dynamics and drug release characteristics.	potential dual responsive hydrogel networks (ppad) are fabricated from pectin, poly((2-dimethylamino)ethyl methacrylate)) and phosphate crosslinker bis[2-methacryloyloxy] ethyl phosphate (bmep) by a simple free radical polymerization. these hydrogel networks are successfully utilized for encapsulation of an anti-cancer drug, 5-fluorouracil (5-fu) and also employed as versatile platforms for production of silver nanoparticles. fabricated hydrogel networks and silver nanocomposites were characteri ...	2017	28576553
evaluation of cytotoxicity of hematite nanoparticles in bacteria and human cell lines.	metal nanoparticles have a great impact and even change the composition of soil microbial communities. this poses the risk of their accumulation in the ecosystem, which may call on health hazard. statistical techniques such as plackett-burman design, response surface methodology were used for optimizing medium constituents for bacillus cereus svk1 and other critical variables responsible for the production of biomolecules and biosynthesis of hematite nanoparticles. the effect of hematite nanopar ...	2017	28586726
a novel technique for sterilization using a power self-regulated single-mode microwave cavity.	in this paper, a novel technique to achieve precise temperatures in food sterilization has been proposed. an accurate temperature profile is needed in order to reach a commitment between the total removal of pathogens inside the product and the preservation of nutritional and organoleptic characteristics. the minimal variation of the target temperature in the sample by means of a monitoring and control software platform, allowing temperature stabilization over 100 °c, is the main goal of this wo ...	2017	28590423
sensitive detection of staphylococcus aureus with vancomycin-conjugated magnetic beads as enrichment carriers combined with flow cytometry.	a novel sandwich strategy was designed to detect staphylococcus aureus. the strategy is based on an antibacterial agent that captures bacterial cells and a fluorescein-labeled antibody that acts as the signal-output probe. vancomycin (van), which exerts a strong antibacterial effect on gram-positive bacteria, was utilized as a molecular recognition agent to detect pathogenic bacteria. to effectively concentrate s. aureus, we used bovine serum albumin (bsa) as the amplification carrier to modify ...	2017	28590745
risk factors of catheter-related bloodstream infection caused by bacillus cereus: case-control study in 8 teaching hospitals in japan.	in this multicenter, matched case-control study, patients diagnosed with catheter-related bloodstream infection (crbsi) caused by bacillus cereus (n = 108) were matched to controls (n = 269). in the multivariable analysis, administration of an amino acid preparation and an indwelling peripheral catheter were significant variables for b cereus-related crbsi.	2017	28596019
a purely green synthesis of silver nanoparticles using carica papaya, manihot esculenta, and morinda citrifolia: synthesis and antibacterial evaluations.	green procedure for synthesizing silver nanoparticles (agnps) is currently considered due to its economy and toxic-free effects. several existing works on synthesizing agnps using leaves extract still involve the use of physical or mechanical treatment such as heating or stirring, which consume a lot of energy. to extend and explore the green extraction philosophy, we report here the synthesis and antibacterial evaluations of a purely green procedure to synthesize agnps using carica papaya, mani ...	2017	28597212
do multi-year applications of bacillus thuringiensis subsp. israelensis for control of mosquito larvae affect the abundance of b. cereus group populations in riparian wetland soils?	bacillus thuringiensis subsp. israelensis (bti) is a soil-borne bacterium affiliated to the bacillus cereus group (bcg) and has been used in biocontrol products against nematoceran larvae for several decades. however, knowledge is limited on whether long-term bti application can affect the structure of indigenous communities of bcg and the overall abundance of bti. using species- and group-specific quantitative pcr assays, we measured the bcg- and bti-abundances in riparian wetlands in the river ...	2017	28600590
an enhancement of antimicrobial efficacy of biogenic and ceftriaxone-conjugated silver nanoparticles: green approach.	of the various methods explored for the synthesis of nanoparticles, biogenesis of silver nanoparticles (agnps) received great attention due to their versatile properties. in this report, daucus carota extract was used for the synthesis of agnps and ceftriaxone was conjugated with agnps to enhance their antimicrobial efficacy. the conjugated and unconjugated agnps were characterized by adopting uv-vis spectroscopy, ftir, afm, dls, and tem, which revealed the spr peak at 420 nm and spherical shape ...	2017	28600792
characterization of hypericum perforatum polysaccharides with antioxidant and antimicrobial activities: optimization based statistical modeling.	in this study, the extracting parameters of crude polysaccharides (cpss) from the hypericum perforatum (hp) including extraction time (eti, 60-180min), extraction temperature (ete, 60-90°c), and the water/solid ratio (w/s, 10-30 was optimized by using three-variable-three-level box-behnken design-response surface methodology (bbd-rsm) based on the single-factor experiments. the optimal extraction conditions were as follow: eti 117.5min, ete 74.28°c, and w/s 20.3:1. under these conditions, the ex ...	2017	28602988
isolation and preliminary characterization of a bacteriocin-producer bacillus strain inhibiting methicillin resistant staphylococcus aureus.	in a multivalent approach to discover new antimicrobial substance, a total of 160 bacilli were isolated from termitarium soil, characterized on the basis of their morphological and physiological characters and screened for their antimicrobial activity by agar well diffusion method against certain drug resistant pathogenic bacteria such as staphylococcus aureus, methicillin resistant staphylococcus aureus and common food contaminating bacteria listeria monocytogenes. after preliminary screening, ...	2017	28605978
nmr structure of the bacillus cereus hemolysin ii c-terminal domain reveals a novel fold.	in addition to multiple virulence factors, bacillus cereus a pathogen that causes food poisoning and life-threatening wound infections, secretes the pore-forming toxin hemolysin ii (hlyii). the hlyii toxin has a unique 94 amino acid c-terminal domain (hlyiic). hlyiic exhibits splitting of nmr resonances due to cis/trans isomerization of a single proline near the c-terminus. to overcome heterogeneity, we solved the structure of p405m-hlyiic, a mutant that exclusively stabilizes the trans state. t ...	2017	28607368
synthesis of new thiazolyl coupled pyrazoles bearing 2,4-thiazolidinedionyl pharmacophore and their anti-inflammatory and antibacterial evaluation.	2, 4-thiazolidinedione (tzd), pyrazole and thiazole heterocyclic rings exhibit wide range of pharmacological activities. medicinal chemists use these heterocyclic moieties as scaffolds in drug designing and discovery. the existing medicaments, celecoxib and meloxicam, used for treating inflammation and pain are having, pyrazole and thiazole, respectively as a key scaffolds. pyrazoles coupled with 2, 4-thiazolidinediones may display enhanced anti inflammatory activity. with this hope the title wo ...	2017	28618987
isolation, morphological identification and in vitro antibacterial activity of endophytic bacteria isolated from azadirachta indica (neem) leaves.	the objective of this study was to isolate endophytic bacteria from azadirachta indica (neem) leaves, their identification and investigate their antibacterial activity against three gram-positive bacteria, staphylococcus aureus, streptococcus pyogenes and bacillus cereus and gram-negative bacteria escherichia coli, salmonella typhimurium and klebsiella pneumoniae.	2017	28620254
assembly and function of the bacillus anthracis s-layer.	bacillus anthracis, the anthrax agent, is a member of the bacillus cereus sensu lato group, which includes invasive pathogens of mammals or insects as well as nonpathogenic environmental strains. the genes for anthrax pathogenesis are located on two large virulence plasmids. similar virulence plasmids have been acquired by other b. cereus strains and enable the pathogenesis of anthrax-like diseases. among the virulence factors of b. anthracis is the s-layer-associated protein bsla, which endows ...	2017	28622090
synthesis, antimicrobial, antiquorum-sensing and antitumor activities of new benzimidazole analogs.	new benzimidazole analogs were prepared and tested for antimicrobial efficacy toward escherichia coli atcc 12435, bacillus cereus uw 85, staphylococcus aureus atcc 29213, candida albicans and aspergillus fumigatus 293. results indicated that compound 10 has potent and broad spectrum antimicrobial activity. in addition, 4b and 5c showed eminent antimicrobial efficacy toward b. cereus, s. aureus, c. albicans and a. fumigatus. furthermore, 12 and 14 demonstrated interesting antifungal activity towa ...	2017	28623814
rapid, high-throughput identification of anthrax-causing and emetic bacillus cereus group genome assemblies using btyper, a computational tool for virulence-based classification of bacillus cereus group isolates using nucleotide sequencing data.	the bacillus cereus group comprises nine species, several of which are pathogenic. differentiating between isolates that may cause disease and those that do not is a matter of public health and economic importance, but can be particularly challenging due to the high genomic similarity of the group. to this end, we have developed btyper, a computational tool that employs a combination of (i) virulence gene-based typing, (ii) multi-locus sequence typing (mlst), (iii) panc clade typing, and (iv) rp ...	2017	28625989
evaluation of in vitro cytotoxic activity of mono-pegylated stap3 (solanum tuberosum aspartic protease 3) forms.	stap3 is a plant aspartic protease with cytotoxic activity toward a broad spectrum of pathogens, including potato and human pathogen microorganisms, and cancer cells, but not against human t cells, human red blood cells or plant cells. for this reason, stap3 could be a promising and potential drug candidate for future therapies. in this work, the improvement of the performance of stap3 was achieved by means of a modification with peg. the separation of a mono-pegylated stap3 fraction was easily ...	2014	28626641
high level bacterial contamination of secondary school students' mobile phones.	while contamination of mobile phones in the hospital has been found to be common in several studies, little information about bacterial abundance on phones used in the community is available. our aim was to quantitatively determine the bacterial contamination of secondary school students' mobile phones.	2017	28626737
variation analysis of bacterial polyhydroxyalkanoates production using saturated and unsaturated hydrocarbons.	polyhydroxyalkanoates (pha) are efficient, renewable and environment friendly polymeric esters. these polymers are synthesized by a variety of microbes under stress conditions. this study was carried out to check the suitability of waste frying oil in comparison to other oils for economical bioplastic production. six bacterial strains were isolated and identified as bacillus cereus (kf270349), klebsiella pneumoniae (kf270350), bacillus subtilis (kf270351), brevibacterium halotolerance (kf270352) ...	2017	28629970
inactivation of bacillus cereus spores on red chili peppers using a combined treatment of aqueous chlorine dioxide and hot-air drying.	the effect of a combined treatment using aqueous chlorine dioxide (clo2 ) and hot-air drying to inactivate bacillus cereus spores on red chili peppers was evaluated. ten washed and dried pepper samples, each comprising half of a single pepper (capsicum annuum l.), were inoculated with b. cereus spore suspension. the inoculated samples were washed with sodium hypochlorite (naocl; 50, 100, or 200 μg/ml) or clo2 (50, 100, or 200 μg/ml) solution for 1 min and then air-dried (25 ± 1 °c, 47 ± 1% relat ...	2017	28631818
enhanced antibacterial activity of ent-labdane derivatives of salvic acid (7α-hydroxy-8(17)-ent-labden-15-oic acid): effect of lipophilicity and the hydrogen bonding role in bacterial membrane interaction.	in the present study, the antibacterial activity of several ent-labdane derivatives of salvic acid (7α-hydroxy-8(17)-ent-labden-15-oic acid) was evaluated in vitro against the gram-negative bacterium escherichia coli and the gram-positive bacteria staphylococcus aureus and bacillus cereus. for all of the compounds, the antibacterial activity was expressed as the minimum inhibitory concentration (mic) in liquid media and minimum inhibitory amount (mia) in solid media. structure activity relations ...	2017	28644410
in defense of bacillus thuringiensis, the safest and most successful microbial insecticide available to humanity - a response to efsa.	the bacillus cereus group contains vertebrate pathogens such as bacillus anthracis and bacillus cereus and the invertebrate pathogen bacillus thuringiensis. microbial biopesticides based on b. thuringiensis (bt) are widely recognized as being among the safest and least environmentally damaging insecticidal products available. nevertheless, a recent food poisoning incident prompted a european food safety authority review which argued that b. thuringiensis poses a health risk equivalent to b. cere ...	2017	28645183
isolation, selection, and biological characterization research of highly effective electricigens from mfcs for phenol degradation.	the microbial fuel cells (mfcs) are recognized to be highly effective for the biodegradation of phenol. for isolating the phenol-degrading bacteria, the sample containing 500 mg/l phenol was collected from the mfcs. the strain (wl027) was identified basing on the 16s rrna gene analysis and phylogenetic analysis as bacillus cereus. the effects of ph, temperature, concentrations of phenol, heavy metal ions, and salt on the growth of strain as well as the degradation of phenol have been carefully s ...	2017	28646380
milk-originated bacillus cereus sensu lato strains harbouring bacillus anthracis-like plasmids are genetically and phenotypically diverse.	bacillus cereus sensu lato is widely distributed in food products, including raw and processed milk. plasmids often determine bacterial virulence and toxicity, but their role in the evolution of b. cereus sensu lato is only partly known. here, we observed that nearly 8% of b. cereus sensu lato isolates were positive for pxo1-like plasmids and 12% for pxo2-like plasmids in raw and ultra-heat-treated (uht) milk from one dairy plant. however, pxo1-like plasmids were significantly more frequent in r ...	2017	28648290
the antibacterial activity and modes of li-f type antimicrobial peptides against bacillus cereus in vitro.	li-fs are a family of highly potent cyclic lipodepsipeptide antibiotics with a broad antimicrobial spectrum (gram-positive bacteria and fungi). in this study, li-f-type antimicrobial peptides (amp-jsa9) composing of li-f03a, li-f03b, li-f04a, li-f04b and li-f05b were isolated from paenibacillus polymyxa jsa-9. to better understand the antimicrobial mechanism of amp-jsa9, the potency and action(s) of amp-jsa9 against bacillus cereus were examined.	2017	28650559
draft genome sequence of pseudomonas koreensis ci12, a bacillus cereus "hitchhiker" from the soybean rhizosphere.	pseudomonas koreensis ci12 was coisolated with bacillus cereus from a root of a soybean plant grown in a field in arlington, wi. here, we report the draft genome sequence of p. koreensis ci12 obtained by illumina sequencing.	2017	28663293
relapsing bacillus cereus peritonitis in a patient treated with continuous ambulatory peritoneal dialysis.	peritonitis is a severe complication of peritoneal dialysis (pd) due to associated morbidity and mortality. although bacillus cereus is mostly considered as a contaminant, its role as a causative agent in a few cases of pd peritonitis has been documented. peritonitis due to b. cereus has been associated with high rates of catheter removal and resistance to beta-lactam antibiotics.	2014	28663812
biological characterization of the biocontrol agent bacillus amyloliquefaciens cpa-8: the effect of temperature, ph and water activity on growth, susceptibility to antibiotics and detection of enterotoxic genes.	this work focuses on the biological understanding of the biocontrol agent bacillus amyloliquefaciens cpa-8 in order to accomplish the characterization required in the registration process for the development of a microorganism-based product. the tolerance of cpa-8 to grow under different ph-temperature and water activity (a w)-temperature conditions was widely demonstrated. regarding the ph results, optimum growth at the evaluated conditions was observed at 37 °c and ph between 7 and 5. on the c ...	2017	28664219
structure and functional characterisation of a distinctive β-lactamase from an environmental strain emb20 of bacillus cereus.	the rampant use and misuse of antibiotics in human medicine, agriculture and veterinary have become the key contributors to global antimicrobial resistance. one of the significant resistance mechanisms that inactivates antibiotics and impedes treatment of bacterial infections is the expression of β-lactamases. rising evidence of newer variants of β-lactamases in the environment is therefore a serious threat to the presently available antibiotic armoury. the present work describes the purificatio ...	2017	28664525
salicylaldehyde and dihydroisobenzofuran derivatives from the marine fungus zopfiella marina.	two salicylaldehyde derivatives (1 and 2), a hydroxymethylphenol (3), five dihydroisobenzofuran (4-8) derivatives, and a 5-chloro-3-deoxyisoochracinic acid (9), together with a known 3-deoxyisoochracinic acid (10) were isolated from the marine fungus zopfiella marina bcc 18240 (or nbrc 30420). the structures of these compounds were elucidated by extensive spectroscopic analysis. compound 1 showed weak antituberculous activity against mycobacterium tuberculosis h37ra, and antibacterial activity a ...	2017	28669223
resolution of enantiopure (s)-1-(1-napthyl) ethanol from racemic mixture by a novel bacillus cereus isolate.	chiral intermediates have wide application and high demand in pharmaceutical, agricultural, and other biotechnological industries for the preparation of bulk drug substances or fine chemicals. (s)-1-(1-napthyl) ethanol is an important synthetic intermediate of mevinic acid analog and a potential inhibitor of 3-hydroxy methyl glutaryl coenzyme a reductase enzymes which is rate limiting for cholesterol synthesis. the present study focuses on the resolution of (rs)-1-(1-napthyl) ethanol using whole ...	2017	28671310
implications of neuroinvasive bacterial peptides on rodents behaviour and neurotransmission.	neuroinvasive microbes are capable of applying their influences on the autonomic nervous system (ans) of the host followed by the involvement of central nervous system (cns) by releasing extracellular metabolites that may cause alterations in the biochemical and neurophysiological environment. consequently synaptic, neuroendocrine, peripheral immune, neuro-immune, and behavioural responses of the host facilitate the progression of infection. the present study was designed to extrapolate the effe ...	2017	28671588
non-toxin-producing bacillus cereus strains belonging to the b. anthracis clade isolated from the international space station.	in an ongoing microbial observatory investigation of the international space station (iss), 11 bacillus strains (2 from the kibo japanese experimental module, 4 from the u.s. segment, and 5 from the russian module) were isolated and their whole genomes were sequenced. a comparative analysis of the 16s rrna gene sequences of these isolates showed the highest similarity (>99%) to the bacillus anthracis-b. cereus-b. thuringiensis group. the fatty acid composition, polar lipid profile, peptidoglycan ...	2017	28680972
analysis of culturable and non-culturable bacteria and their potential to form biofilms in a primary treated dairy wastewater system.	biofilm formation in a dairy waste irrigation system can reduce treatment capacity and increase maintenance and cleaning. an extreme biofilm observed in a primary treated dairy wastewater system blocked the irrigation line requiring manual cleaning. both next-generation genomic sequencing and the culturable fraction showed the presence of predominantly gram-negative bacteria. isolates identified from current samples and stored samples from the extreme biofilm included pseudomonas, citrobacter, k ...	2017	28681658
molecular ecological network analysis reveals the effects of probiotics and florfenicol on intestinal microbiota homeostasis: an example of sea cucumber.	animal gut harbors diverse microbes that play crucial roles in the nutrition uptake, metabolism, and the regulation of host immune responses. the intestinal microbiota homeostasis is critical for health but poorly understood. probiotics paracoccus marcusii db11 and bacillus cereus g19, and antibiotics florfenicol did not significantly impact species richness and the diversity of intestinal microbiota of sea cucumber, in comparison with those in the control group by high-throughput sequencing. mo ...	2017	28684750
antibacterial and antioxidant constituents of extracts of endophytic fungi isolated from ocimum basilicum var. thyrsiflora leaves.	fourteen fungal endophytes were isolated from the ocimum basilicum var. thyrsiflora leaves collected from northern thailand. eight genera were identified including aspergillus, ascochyta, nigrospora, blastomyces, colletotrichum, exidia, clitopilus, and nomuraea. the antibacterial activity of crude extracts from all endophytic fungi was tested against nine human bacterial pathogens: staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, bacillus cereus, escherichia coli, klebsiella ...	2017	28698913
development and characterization of bioactive edible films from spider crab (maja crispata) chitosan incorporated with spirulina extract.	active food packaging films based on crab chitosan and spirulina extract (se) were developed. the effects of the se incorporation at different levels on physical (color, opacity water vapor and oxygen permeability) and mechanical (tensile strength and elongation at break) properties of chitosan films were investigated. ftir was carried out to observe the potential modifications of the chitosan films when incorporated with se. the obtained results suggested that incorporation of se into chitosan ...	2017	28705500
investigation on the antimicrobial activities of gingers (etlingera coccinea (blume) s.sakai & nagam and etlingera sessilanthera r.m.sm.) endemic to borneo.	to investigate the antimicrobial properties of e. coccinea and e. sessilanthera and to isolate and identify the antimicrobial compounds.	2017	28708293
dynamics of culturable mesophilic bacterial communities of three fresh herbs and their production environment.	investigate dynamics of culturable mesophilic bacteria and selected food-contaminating bacteria from three herbs and their production environment.	2017	28708321
evaluation of nutrients removal (no3-n, nh3-n and po4-p) with chlorella vulgaris, pseudomonas putida, bacillus cereus and a consortium of these microorganisms in the treatment of wastewater effluents.	in this research removal of nh3-n, no3-n and po4-p nutrients from municipal wastewater was studied, using chlorella vulgaris, pseudomonas putida, bacillus cereus and an artificial consortium of them. the objective is to analyze the performance of these microorganisms and their consortium, which has not been previously studied for nutrient removal in municipal wastewater. a model wastewater was prepared simulating the physicochemical characteristics found at the wastewater plant in chapala, mexic ...	2017	28708609
the potential multiple mechanisms and microbial communities in simultaneous nitrification and denitrification process treating high carbon and nitrogen concentration saline wastewater.	a simultaneous nitrification and denitrification (snd) process in sequencing batch biofilm reactor (sbbr) was established to treat high carbon and nitrogen saline wastewater in this study. acetate, glucose and an organic mixture were applied as organic sources in three sbbrs, achieving average total nitrogen removal efficiency of 97.15%, 63.94% and 94.99% during 120days' operation, respectively. the underlying nitrogen removal mechanisms were investigated by 16s rrna sequencing and batch tests. ...	2017	28710998
effect of dairy product environment on the growth of bacillus cereus.	ph is one of the most important parameters to manage bacterial replication in foodstuffs. in this study, the ability of 2 bacillus cereus strains, 1 clinical human isolate (gpe2) and 1 isolate from a dairy product (d43), were investigated for in vitro growth at different ph values (from 3.5 to 7.5) at 2 temperatures (15 and 37°c), showing their ability to grow from 5.5 to 7.5 and from 5.0 to 7.5, respectively. the ability of spores of these 2 microorganisms to germinate in different typologies o ...	2017	28711259
detection of eight foodborne bacterial pathogens by oligonucleotide array hybridization.	simultaneous and rapid detection of multiple foodborne bacterial pathogens is important for the prevention of foodborne illnesses.	2017	28713514
characterization of enterotoxigenic bacillus cereus sensu lato and staphylococcus aureus isolates and associated enterotoxin production dynamics in milk or meat-based broth.	bacillus cereus sensu lato species, as well as staphylococcus aureus, are important pathogenic bacteria which can cause foodborne illness through the production of enterotoxins. this study characterised enterotoxin genes of these species and examined growth and enterotoxin production dynamics of isolates when grown in milk or meat-based broth. all b. cereus s. l. isolates harboured nhea, hbla and entfm toxin genes, with lower prevalence of bcet and hlyii. when grown at 16 °c, toxin production by ...	2017	28714887
sinonasal t2r-mediated nitric oxide production in response to bacillus cereus.	upper airway epithelial cells produce bactericidal nitric oxide (no) in response to both gram-positive and gram-negative bacteria. our previous work demonstrated that t2r38, a bitter taste receptor (t2r) expressed in airway epithelium, produces no in response to quorum-sensing molecules secreted by pseudomonas aeruginosa. we also demonstrated that staphylococci products elicit an no response when using a t2r-independent pathway. when screening additional human pathogens for epithelial t2r activa ...	2017	28716170
an excited state underlies gene regulation of a transcriptional riboswitch.	riboswitches control gene expression through ligand-dependent structural rearrangements of the sensing aptamer domain. however, we found that the bacillus cereus fluoride riboswitch aptamer adopts identical tertiary structures in solution with and without ligand. using chemical-exchange saturation transfer (cest) nmr spectroscopy, we revealed that the structured ligand-free aptamer transiently accesses a low-populated (∼1%) and short-lived (∼3 ms) excited conformational state that unravels a con ...	2017	28719589
isolation and characterization of lactic acid bacteria from traditional pickles of himachal pradesh, india.	a total of 15 isolates of lactic acid bacteria (lab) were isolated from traditional pickles of rural and urban areas of himachal pradesh. these isolates were identified as enterococcus faecalis (7), lactobacillus plantarum (3), pediococcus pentosaceus (2), leuconostoc mesenteroides (1), lactococcus lactis (1) and enterococcus sp. (1) on the basis of morphological, biochemical and 16s rdna sequence analysis. all the labs produced exopolysaccharides, have antibiotic susceptibility, and possess act ...	2017	28720951
chemical constituents of helichrysum italicum (roth) g. don essential oil and their antimicrobial activity against gram-positive and gram-negative bacteria, filamentous fungi and candida albicans.	the aerial parts of helichrysum italicum (roth) g. don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. the essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). the most o ...	2017	28725151
effects of secondary metabolites of permafrost bacillus sp. on cytokine synthesis by human peripheral blood mononuclear cells.	we studied the effects of secondary metabolites of bacillus sp. isolated from late neogene permafrost on secretion of proinflammatory (tnf-α, il-1β, il-8, il-2, and ifnγ) and antiinflammatory (il-4 and il-10) cytokines by human peripheral blood mononuclear cells. it was found that metabolites of bacillus sp. produced more potent effect on cytokine secretion than mitogen phytohemagglutinin and metabolites of bacillus cereus, medicinal strain ip5832. activity of metabolites depended on the tempera ...	2017	28726195
complete genome sequences of three phi29-like bacillus cereus group podoviridae.	three double-stranded dna phi29-like bacillus cereus group bacteriophages, beachbum, harambe, and serpounce, were identified and characterized. beachbum and harambe are closely related but are remarkably different from previously identified phi29-like phages. serpounce is substantially closer to other phi29-like phages, enabling the identification of its prohead rna (prna) gene.	2017	28729280
protease hydrolysates of filefish (thamnaconus modestus) byproducts effectively inhibit foodborne pathogens.	in this study, novel antimicrobial peptides had been derived by enzymatic hydrolysis of filefish (thamnaconus modestus) byproduct (hfbp). different proteases, (papain [p], trypsin [t], neutrase [n], pepsin [pe], and the mixture i [pn] and mixture ii [pt]) and different hydrolysis time (60, 120, 180, and 240 min), have been used to generate peptides with different lengths and amino acid sequences. the antimicrobial properties of hfbps were tested, against gram-positive and gram-negative strains, ...	2017	28731389
crystal structures of an atypical aldehyde dehydrogenase having bidirectional oxidizing and reducing activities.	aldehyde dehydrogenases (aldhs) are nad(p)(+)-dependent oxidoreductases that catalyze the oxidation of a variety of aldehydes to their acid forms. in this study, we determined the crystal structures of aldh from bacillus cereus (bcaldh), alone, and in complex with nad(+) and nadp(+). this enzyme can oxidize all-trans-retinal to all-trans-retinoic acid using either nad(+) or nadp(+) with equal efficiency, and atypically, as a minor activity, can reduce all-trans-retinal to all-trans-retinol using ...	2017	28732729
antibacterial activity of fusion from biosynthesized acidocin/silver nanoparticles and its application for eggshell decontamination.	bacteriocins from lactic acid bacteria (lab) are useful to control the persistent development of pathogenic microorganisms in food and medicine fields. the bacteriocin acidocin was extracted from lactobacillus acidophilus m1 that was isolated from fermented milk, purified using ammonium sulphate fractionation, and gel filtration column chromatography using sephadex matrix and applied as a potential antibacterial agent. the molecular weight of the purified acidocin was estimated using sds-page to ...	2017	28736866
bam35 tectivirus intraviral interaction map unveils new function and localization of phage orfan proteins.	tectiviridae comprises a group of tail-less, icosahedral, membrane-containing bacteriophages that can be divided into two groups by their hosts, either gram-negative or gram-positive bacteria. while the first group is composed of prd1 and nearly identical well characterized lytic viruses, the second one includes more variable temperate phages, like gil16 or bam35, whose hosts are bacillus cereus and related gram-positive bacteria.in the genome of bam35, nearly half of the 32 annotated open readi ...	2017	28747494
quantitative prevalence, phenotypic and genotypic characteristics of bacillus cereus isolated from retail infant foods in china.	bacillus cereus is an important foodborne pathogen, which can cause severe food poisoning. the aim of this study was (i) to evaluate the quantitative prevalence of b. cereus in retail prepackaged infant formula and ready-to-eat rice flour in china and (ii) to gain the basic information on pheno- and genotypic characteristics of b. cereus isolates. we found that 40 out of the 587 samples were positive for b. cereus. b. cereus in 3.5% of infant formula samples and 1.0% of rice flour samples outnum ...	2017	28753035
cyclohexene-fused 1,3-oxazines with selective antibacterial and antiparasitic action and low cytotoxic effects.	oxazine derivatives, a class of heterocyclic compounds, exhibit a variety of biological properties, such as anticonvulsant and antitumor activities. in this study, we evaluated the effect of two cyclohexene-fused 1,3-oxazines (cis‑1-benzyl-n-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (1) and trans‑n-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (2)) in cultures of bacillus cereus, enterococcus faecalis, escherichia coli, klebsiella pneumoniae, salmonella enterica, serratia mar ...	2017	28755871
multiplex pcr coupled with propidium monoazide for the detection of viable cronobacter sakazakii, bacillus cereus, and salmonella spp. in milk and milk products.	cronobacter sakazakii, bacillus cereus, and salmonella spp. are common food-borne pathogens. the aim of this study was to develop a sensitive, specific, and rapid method for the simultaneous detection of these 3 pathogens in milk and milk products. three specific primers were designed based on ompa, inva, and cesb of c. sakazakii, salmonella spp. and b. cereus, respectively, for use in a multiplex pcr (mpcr). to eliminate false-positive results, cells were pretreated with propidium monoazide (pm ...	2017	28755939
formulation of menthol-loaded nanostructured lipid carriers to enhance its antimicrobial activity for food preservation.	purpose: due to the antimicrobial property, menthol have significant potential for food preservation and foodstuff shelf life improvement. nevertheless, menthol instability, insolubility, and rapid crystallization in aqueous media make it unsuitable for used in food products. this work was aimed to prepare menthol-loaded nanostructured lipid carriers (nlcs) to enhance its antimicrobial activity. methods: morphology, particle size and size distribution, encapsulation efficiency percent (ee%), and ...	2017	28761828
purification and characterization of trypsin produced by gut bacteria from anticarsia gemmatalis.	purification of active trypsin in the digestive process of insects is essential for the development of potent protease inhibitors (pis) as an emerging pest control technology and research into insect adaptations to dietary pis. an important aspect is the presence of proteolytic microorganisms, which contribute to host nutrition. here, we purified trypsins produced by bacteria bacillus cereus, enterococcus mundtii, enterococcus gallinarum, and staphylococcus xylosus isolated from the midgut of an ...	2017	28762531
phenolic acids and flavonoids of peanut by-products: antioxidant capacity and antimicrobial effects.	peanut skin (ps) and meal from dry-blanched peanuts (mdbp) were evaluated as sources of phenolic compounds. ps rendered the highest total phenolic content, antioxidant capacity towards abts radical cation, dpph and hydroxyl radicals as well as reducing power. phenolic acids were present in ps and mdbp whereas proanthocyanidins and monomeric flavonoids were found only in ps as identified by hplc-dad-esi-ms(n). procyanidin-rich extracts prevented oxidation in non-irradiated and gamma-irradiated fi ...	2017	28764032
pan-genome and phylogeny of bacillus cereus sensu lato.	bacillus cereus sensu lato (s. l.) is an ecologically diverse bacterial group of medical and agricultural significance. in this study, i use publicly available genomes and novel bioinformatic workflows to characterize the b. cereus s. l. pan-genome and perform the largest phylogenetic and population genetic analyses of this group to date in terms of the number of genes and taxa included. with these fundamental data in hand, i identify genes associated with particular phenotypic traits (i.e., "pa ...	2017	28768476
persistent anthrax as a major driver of wildlife mortality in a tropical rainforest.	anthrax is a globally important animal disease and zoonosis. despite this, our current knowledge of anthrax ecology is largely limited to arid ecosystems, where outbreaks are most commonly reported. here we show that the dynamics of an anthrax-causing agent, bacillus cereus biovar anthracis, in a tropical rainforest have severe consequences for local wildlife communities. using data and samples collected over three decades, we show that rainforest anthrax is a persistent and widespread cause of ...	2017	28770842
bacillus cereus group bacteriophage flapjack genome sequence.	the bacillus cereus group bacteriophage flapjack, a double-stranded dna (dsdna) myoviridae isolate collected from soil collected in washington, dc, is a member of cluster c3 and encodes an intramolecular chaperone-containing tail fiber protein previously found in podoviridae and siphoviridae but not annotated in the myoviridae.	2017	28774974
antimicrobial activity of endophytic bacterial populations isolated from medical plants of iran.	endophytic actinobacteria colonize inside the plant tissues without causing damages to the host plant. since these microorganisms colonize in the different parts of plants and can stop plant disease, they have been applied as biological agents for controlling human diseases. the aim of this study was molecular identification and measuring the antimicrobial activity of endophytic actinomycetes isolated from medicinal plants of iran.	2017	28775818
gene expression and in silico analysis of snakehead murrel interleukin 8 and antimicrobial activity of c-terminal derived peptide ws12.	chemokines have been known for their wide range of functions including chemoattractant property in humans and other vertebrate organisms. they act as a bridge between innate and adaptive immune system. in the present study, we have identified a cxc chemokine from the cdna library of c. striatus; on the basis of orthology study, it was found highly identical to interleukin 8 (il8). the bioinformatics analysis of the chemokine revealed the presence of a typical γ-core domain and a cxc motif at the ...	2017	28778316
novel motb as a potential predictive tool for identification of b. cereus, b. thuringiensis and differentiation from other bacillus species by triplex real-time pcr.	quantitative triplex real-time pcr (qpcr) offers an alternative method for detection of bacterial contamination. it provides quantitation of the number of gene copies. in our study, we established a qpcr assay to detect and quantify the specificity towards bacillus cereus and b. thuringiensis. the assay was designed to detect a 280 bp fragment of motb gene encoding the flagellar motor protein, specific for detection of b. cereus and b. thuringiensis, excluding other group species b. pseudomycoid ...	2017	28778821
expression and purification of transmembrane protein mere from mercury resistant bacillus cereus.	mercury resistant (hg(r)) bacteria were isolated from heavy metal polluted wastewater and soil collected near to tanneries of district kasur, pakistan. bacterial isolates az-1, az-2 and az-3 showed resistance up to 40 μg/ml against mercuric chloride (hgcl2). 16s rdna ribotyping and phylogenetic analysis were performed for the characterization of selected isolates as bacillus sp. az-1 (kt270477), bacillus cereus az-2 (kt270478) and bacillus cereus az-3 (kt270479). phylogenetic relationship on the ...	2017	28783894
comparative genomics of bacillus thuringiensis reveals a path to specialized exploitation of multiple invertebrate hosts.	understanding the genetic basis of host shifts is a key genomic question for pathogen and parasite biology. the bacillus cereus group, which encompasses bacillus thuringiensis and bacillus anthracis, contains pathogens that can infect insects, nematodes, and vertebrates. since the target range of the essential virulence factors (cry toxins) and many isolates is well known, this group presents a powerful system for investigating how pathogens can diversify and adapt to phylogenetically distant ho ...	2017	28790205
proposal of nine novel species of the bacillus cereus group.	nine novel gram-stain-positive bacteria were investigated by a polyphasic taxonomic approach. based on the analysis of 16s rrna gene sequences, these strains belonged to the bacillus cereus group, sharing over 97 % similarity with the known species of this group, and less than 95 % similarity with other species of the genus bacillus. multilocus sequence typing analysis showed that they formed nine robust and well-separated branches from the known species. the digital dna-dna hybridization (dddh) ...	2017	28792367
potassium-ion-selective fluorescent sensors to detect cereulide, the emetic toxin of b. cereus, in food samples and hela cells.	we report the development of new chemical probes for cereulide, a toxic metabolite produced by specific strains of bacillus cereus, through displacement of potassium cations from a preformed specific complex and a subsequent change in the fluorescence emission. for this purpose, we designed fluorescent probes for potassium cations that were suitable for displacement assays with cereulide from organic extracts. the fluorescence detection of natural cereulide in rice samples was achieved by using ...	2017	28794952
draft genome sequences from a novel clade of bacillus cereus sensu lato strains, isolated from the international space station.	the draft genome sequences of six bacillus strains, isolated from the international space station and belonging to the bacillus anthracis-b. cereus-b. thuringiensis group, are presented here. these strains were isolated from the japanese experiment module (one strain), u.s. harmony node 2 (three strains), and russian segment zvezda module (two strains).	2017	28798168
methionine residues in exoproteins and their recycling by methionine sulfoxide reductase ab serve as an antioxidant strategy in bacillus cereus.	during aerobic respiratory growth, bacillus cereus is exposed to continuously reactive oxidant, produced by partially reduced forms of molecular oxygen, known as reactive oxygen species (ros). the sulfur-containing amino acid, methionine (met), is particularly susceptible to ros. the major oxidation products, methionine sulfoxides, can be readily repaired by methionine sulfoxide reductases, which reduce methionine sulfoxides [met(o)] back to methionine. here, we show that methionine sulfoxide re ...	2017	28798727
growth of amanita caesarea in the presence of pseudomonas fluorescens and bacillus cereus.	the ectomycorrhizal (ecm) fungus amanita caesarea cect 20127 was tested in vitro with two potentially mycorrhizal-promoting bacterial strains, pseudomonas fluorescens cect 844 and bacillus cereus cect 148. although p. fluorescens showed spatial and temporal compatibility with a. caesarea, it did not affect growth of the fungus. conversely, b. cereus exhibited no such compatibility and also inhibited fungal growth. the expression pattern of the a. caesarea gene acmst-1 was analysed using real-tim ...	2017	28800852
simultaneous removal of nitrogen and phosphorous from domestic wastewater using bacillus cereus gs-5 strain exhibiting heterotrophic nitrification, aerobic denitrification and denitrifying phosphorous removal.	a newly isolated gs-5 strain exhibiting heterotrophic nitrification, aerobic denitrification and denitrifying phosphorous removal was identified as bacillus cereus gs-5 based on its phenotypic and phylogenetic characteristics. the isolate had exhibited efficient nh4(+)-n, no3(-)-n, no2(-)-n and po4(3-)-p removal from nutrient spiked real domestic wastewater with average rates of 2.62, 2.69, 1.16 and 0.42mgl(-1)h(-1), respectively under aerobic condition. metabolic inhibitor based mass balance an ...	2017	28803098
the antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human hel 12469 cells.	six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. antimicrobial activity was shown against pathogenic (escherichia coli, salmonella typhimurium, yersinia enterocolitica, staphylococcus aureus, listeria monocytogenes, and enterococcus faecalis) and environmental bacteria (bacillus cereus, arthrobacter protophormiae, pseudomonas fragi) and fungi (chaetomium globosum, penicillium chrysogenum, cladospori ...	2017	28811611
synergistic antibacterial and antibiofilm efficacy of nisin in combination with p-coumaric acid against foodborne bacteria bacillus cereus and salmonella typhimurium.	the aim of the study was to evaluate possible antibacterial and antibiofilm efficacy of a bacteriocin, nisin with two essential oil components linalool and p-coumaric acid in combination against foodborne bacteria bacillus cereus and salmonella typhimurium. their inhibition effects on planktonic cells and pre-formed biofilms were evaluated using microbroth dilution and checkerboard titration methods. nisin/ p-coumaric acid combination showed synergistic effects against planktonic cells of both t ...	2017	28815637
discovery of a potent inhibitor class with high selectivity toward clostridial collagenases.	secreted virulence factors like bacterial collagenases are conceptually attractive targets for fighting microbial infections. however, previous attempts to develop potent compounds against these metalloproteases failed to achieve selectivity against human matrix metalloproteinases (mmps). using a surface plasmon resonance-based screening complemented with enzyme inhibition assays, we discovered an n-aryl mercaptoacetamide-based inhibitor scaffold that showed sub-micromolar affinities toward coll ...	2017	28820255
biological activity of the non-microbial fraction of kefir: antagonism against intestinal pathogens.	kefir is a fermented milk obtained by the activity of kefir grains which are composed of lactic and acetic acid bacteria, and yeasts. many beneficial health effects have been associated with kefir consumption such as stimulation of the immune system and inhibition of pathogenic microorganisms. the biological activity of kefir may be attributed to the presence of a complex microbiota as well as the microbial metabolites that are released during fermentation. the aim of this work was to characteri ...	2017	28831964
structural and immunochemical relatedness suggests a conserved pathogenicity motif for secondary cell wall polysaccharides in bacillus anthracis and infection-associated bacillus cereus.	bacillus anthracis (ba) and human infection-associated bacillus cereus (bc) strains bc g9241 and bc 03bb87 have secondary cell wall polysaccharides (scwps) comprising an aminoglycosyl trisaccharide repeat: →4)-β-d-manpnac-(1→4)-β-d-glcpnac-(1→6)-α-d-glcpnac-(1→, substituted at glcnac residues with both α- and β-galp. in bc g9241 and bc 03bb87, an additional α-galp is attached to o-3 of mannac. using nmr spectroscopy, mass spectrometry and immunochemical methods, we compared these structures to s ...	2017	28832613
antimicrobial and antioxidant activities of the extract and fractions of tetradenia riparia (hochst.) codd (lamiaceae) leaves from brazil.	tetradenia riparia (lamiaceae) is native to central africa popularly known as myrrh, used in folk medicine to treat various diseases like malaria, gastroenteritis, and tropical skin disease. this research was to evaluate the antioxidant and antibacterial activities of the crude extract (ce) and fractions (fr) of the t. riparia by classical chromatography. the ce of t. riparia leaves was submitted to column chromatographic fractionation to obtain four fractions of the interest, which were identif ...	2017	28840299
levels of l-malate and other low molecular weight metabolites in spores of bacillus species and clostridium difficile.	dormant spores of bacillus species lack atp and nadh and contain notable levels of only a few other common low mol wt energy reserves, including 3-phosphoglyceric acid (3pga), and glutamic acid. recently, bacillus subtilis spores were reported to contain ~ 30 μmol of l-malate/g dry wt, which also could serve as an energy reserve. in present work, l-malate levels were determined in the core of dormant spores of b. subtilis, bacillus cereus, bacillus megaterium and clostridium difficile, using bot ...	2017	28850573
complete genome sequence of the nematicidal bacillus thuringiensis mybt18246.	10.1601/nm.5000 is a rod-shaped facultative anaerobic spore forming bacterium of the genus 10.1601/nm.4857. the defining feature of the species is the ability to produce parasporal crystal inclusion bodies, consisting of δ-endotoxins, encoded by cry-genes. here we present the complete annotated genome sequence of the nematicidal 10.1601/nm.5000 strain mybt18246. the genome comprises one 5,867,749 bp chromosome and 11 plasmids which vary in size from 6330 bp to 150,790 bp. the chromosome contains ...	2017	28852435