phosphonate monoester inhibitors of class a beta-lactamases.phosphonate monoesters with the general structure: [formula: see text] are inhibitors of representative class a and class c beta-lactamases. this result extends the range of this type of inhibitor to the class a enzymes. compounds where x is an electron-withdrawing substituent are better inhibitors than the unsubstituted analogue (x = h), and enzyme inhibition is concerted with stoichiometric release of the substituted phenol. slow turnover of the phosphonates also occurs. these observations sup ...19911903928
a continuous fluorescence-displacement assay for triacylglycerol lipase and phospholipase c that also allows the measurement of acylglycerols.a new continuous fluorescence-displacement assay for enzymes that release long-chain fatty acids [wilton (1990) biochem. j. 266, 435-439] is described in detail for pig pancreatic triacylglycerol lipase. the assay involves the displacement of the highly fluorescent fatty acid probe 11-(dansylamino)undecanoic acid from rat liver fatty acid-binding protein by long-chain fatty acids released as a result of enzyme activity. the assay is surprisingly effective for triacylglycerol lipase, allowing the ...19911903930
one-step purification procedure for udp-n-acetylmuramyl-peptide murein precursors from bacillus cereus.a method is described for the rapid isolation of the activated murein precursors udp-n-acetyl-muramyl-pentapeptide (udp-murnac-pentapeptide) and udp-murnac-tripeptide from bacillus cereus. after accumulation of the precursors by inhibition of murein synthesis either in the presence of vancomycin (for the pentapeptide precursor) or d-cycloserine (for the tripeptide precursor) the cells were extracted with boiling water. prior to high pressure liquid chromatography the material was freed from acid ...19911904044
nonanthrax bacillus infections of the central nervous system. 19911904148
effect of phenolic compounds and oleuropein on the germination of bacillus cereus t spores.the phenolic compounds extracted from olives with ethyl acetate inhibited germination and outgrowth of bacillus cereus t spores. purified oleuropein, a well-characterized component of olive extract, inhibited these processes also. the addition of oleuropein and olive extracts 3 or 5 min after germination began, immediately decreased the rate of change of phase bright to phase dark spores and delayed significantly outgrowth.19911904245
site-directed mutagenesis of dicarboxylic acids near the active site of bacillus cereus 5/b/6 beta-lactamase amino acid residue functioning as a general base has been proposed to assist in the hydrolysis of beta-lactam antibiotics by the zinc-containing bacillus cereus beta-lactamase ii [bicknell & waley (1985) biochemistry 24, 6876-6887]. oligonucleotide-directed mutagenesis of cloned bacillus cereus 5/b/6 beta-lactamase ii was used in an 'in vivo' study to investigate the role of carboxy-group-containing amino acids near the active site of the enzyme. substitution of asparagine for the wild-type a ...19911904717
assays of phospholipases on short-chain phospholipids.short-chain phospholipids are extremely useful compounds for analysis of interfacial and substrate requirements of water-soluble phospholipases. a wide variety of pcs are easily prepared and can be characterized in detail to the point where at a given pc concentration, the micelle size distribution is known, and an area per head group can be estimated. since they can be presented to enzymes as monomers, micelles, or in bilayer structures, short-chain phospholipids are ideal for examining kinetic ...19911904976
differentiation of dairy strains of the bacillus cereus group by phage typing, minimum growth temperature, and fatty acid analysis.a total of 130 bacillus strains were isolated from dairy products, the dairy environment and from packaging boards and board-producing machines. ninety-eight of these were members of the b. cereus group (b. cereus, b. mycoides and b. thuringiensis) as determined by whole cell fatty acid composition. fatty acid composition did not differentiate between the three species. of the 98 strains, which were indistinguishable by biochemical tests, 87 could be assigned into 21 different phage types (11 st ...19911905282
growth of bacillus cereus in fermenting tempeh made from various beans and its inhibition by lactobacillus plantarum.counts of bacillus cereus reached ca 10(8) cfu/g within 40 h in fermenting unacidified horsebean tempeh and resulted in complete spoilage of the product. in fermenting unacidified pea, chickpea and soybean tempeh, b. cereus counts reached 10(6)-10(7) cfu/g, although the products were not spoiled. inoculation of these unacidified beans with lactobacillus plantarum decreased the final count of b. cereus by 2 log units, but had no effect on its growth in unacidified horsebean tempeh and its subsequ ...19911905283
identity of hemolysins produced by bacillus thuringiensis and bacillus cereus.a hemolysin (bt-hemolysin) produced by bacillus thuringiensis var. kurstaki hd-1 producing crystalline toxin(s) was purified by successive treatments of ammonium sulfate (45-65%) and column chromatography using deae-cellulose, sephadex g-75 and kb-002 (a hydroxyapatite column for fast protein liquid chromatography). a hemolysin (bc-hemolysin) produced by b. cereus hg-6a was also purified by the same procedure. the purified bt-hemolysin and bc-hemolysin, both of which are thiol-activated hemolysi ...19911905656
irreversible inactivation of beta-lactamase i from bacillus cereus by chlorinated 6-spiroepoxypenicillins.on incubation of the chlorinated 6-spiroepoxypenicillin anilides (i) and (ii) [formula: see text] with beta-lactamase 1 from bacillus cereus, three distinct processes are observed. the inhibitors act as (a) substrates, the turnover of which respectively results in a single product, namely 6-substituted 2(h)-3,4-dihydro-1,4-thiazine, (b) a transiently inhibited enzyme complex, and finally (c) an irreversibly inactivated enzyme complex. although differing only in their stereochemistry at one centr ...19911905929
antimicrobial activity of microgard against food spoilage and pathogenic microorganisms.microgard, a commercially available fermented milk product containing antimicrobial metabolites, was a potent inhibitor for gram-negative bacteria such as pseudomonas, salmonella, and yersinia when 1% concentration was incorporated into agar media. gram-positive bacillus cereus, staphylococcus aureus, and listeria monocytogenes were insensitive to microgard. kluyveromyces marxianus, an unidentified black yeast, and penicillium expansum were partially suppressed, whereas aspergillus niger and a y ...19911906486
bacillus cereus pneumonia with empyema complicating aplastic anemia--a case report. 19911906515
improved methods for purification of an enterotoxin produced by bacillus enterotoxin (et) produced by bacillus cereus was purified by ammonium sulfate precipitation followed by chromatography on sp-sephadex c-25, chromatofocusing, and gel filtration on sephacryl s-300. purified et was electrophoretically homogeneous with a molecular mass of 45,000 and with an isoelectric point of 5.5. it showed vascular permeability activity in rabbits, was lethal to mice, and caused fluid accumulation in mouse ligated intestinal loops. it showed cytotoxicity to vero cells, but di ...19911906824
[the effect of a cationic surface-active substance (catamine ab) on the physiological-morphological properties of b. cereus and e. coli].catamine ab (0.05-0.5%) promotes transfer of b. cereus st 96, and e. coli st. 906 cell cultures into metabolic rest. detergent-treated cells have no energetic metabolism and autolytic processes, have high light-scattering coefficient, and peculiar ultrastructural organization. viable cells can be observed in the detergent-treated cell suspension after 1 year of incubation. difference in action of different catamine ab concentrations on stationary and exponential b. cereus cells has been revealed ...19911906901
inhibition of clostridium tyrobutyricum by bacteriocin-like substances produced by lactic acid bacteria.lactic acid bacteria were selected for their inhibitory activity against clostridium tyrobutyricum under conditions that eliminate the effects of lactic acid and hydrogen peroxide. four strains were isolated belonging to the species lactococcus lactis ssp. lactis. the sensitivity of the inhibitory substances to pronase and trypsine indicates that they are proteins or peptides different from nisin. their resistance to phospholipase d indicates that they are also different from lactostrepcin. the ...19911907300
influence of cellular differentiation on ultraviolet induced dna damage and its repair mechanisms in b. cereus.susceptibility to uv irradiation of b. cereus bis-59 spores undergoing germination at various stages-dormant spores to vegetative cell stage and their ability to recover from radiation damage were studied. for a given dose of radiation, the number of spore photoproducts (spp) formed in the dna of dormant spores was about 5-times greater than that of thymine dimers (tt) formed in the dna of vegetative cells. at intermediate stages of the germination cycle, there was a rapid decline in the uv radi ...19911908819
differentiation of bacillus anthracis from bacillus cereus by gas chromatographic whole-cell fatty acid analysis.three strains of bacillus anthracis and seven strains of bacillus cereus were grown on complex medium and on synthetic medium. gas chromatographic analysis of whole-cell fatty acids of strains grown on complex medium gave nearly identical fatty acid patterns. fatty acid patterns of strains grown on synthetic medium showed a high content of branched-chain fatty acids. significant differences between the fatty acid patterns of the two species were found. odd iso/anteiso fatty acid ratios were abou ...19911909348
the occurrence and growth of microorganisms during the fermentation of fish sausage.minced fish (mullet) sausage mixes containing added sugar, salt, nitrate, nitrite and spices were fermented (48 h, 30 degrees c) by indigenous flora or by a starter culture (pediococcus acidilactici) and the microbial ecology and behaviour of various bacteria was monitored. pediococcus pentosaceus and lactobacillus plantarum dominated the indigenous fermentation, achieving populations of 10(7)-10(8) cfu/g by 48 h, and decreasing the ph of the mix to 4.5-4.7. significant growth (10(5)-10(7) cfu/g ...19911909546
microbial conversion of fungicide ecological safety study of using vinclozolin in field and laboratory experiments showed that the effect of the preparation led to a decrease in the abundance of actinomycetes and mycelial fungi and an enhancement of nitrification. the residual amounts of vinclozolin in soil after 12 months were 6-12% of the dose introduced. the persistent chlorinated derivatives of the toxicant were found. microbial strains pertaining to the genera pseudomonas and bacillus were isolated that utilized vinclozo ...19911910065
overproduction, purification and crystallization of bacillus cereus oligo-1,6-glucosidase.the gene coding for oligo-1,6-glucosidase from bacillus cereus atcc7064 has been overexpressed in escherichia coli mv1184 cells under the control of the lac promoter in the genetically engineered plasmid pbce4-2. oligo-1,6-glucosidase was purified in large quantities and was crystallized at 25 degrees c by using a hanging drop vapor diffusion method with 53% saturated ammonium sulfate. the crystals have the shape of hexagonal bipyramids and belong to the space group p6(2) or p6(4) with lattice c ...19911915879
bacteria in food packaging paper and board.the bacteria of food packaging paper and board were studied. most of the aerobic strains were spore-formers; members of the genus bacillus with b. cereus group (b. cereus, b. mycoides, b. thuringiensis), b. polymyxa group (b. polymyxa, b. circulans, b. macerans, b. pabuli), b. brevis and b. licheniformis predominated. the main source of spore-forming bacteria in paper and board was the broke (rejected paper or board, which is repulped and recycled into the process). gram-negative bacteria were r ...19911917722
symposium on microbiology update: old friends and new enemies. bacillus cereus.bacillus cereus is an environmentally ubiquitous, gram-positive, spore-forming bacillus responsible for 2 distinct foodborne disease syndromes as well as other manifestations of pathogenicity. the rapid-onset, "emetic," foodborne-disease syndrome is associated with an emetic toxin; the delayed-onset, "diarrheal" syndrome is associated with elaboration of enterotoxin. the majority of methods for detection of these toxins have relied on in vivo testing. more recent work on purification of enteroto ...19911917820
ultraviolet irradiation of dna complexed with alpha/beta-type small, acid-soluble proteins from spores of bacillus or clostridium species makes spore photoproduct but not thymine dimers.uv irradiation of complexes of dna and an alpha/beta-type small, acid-soluble protein (sasp) from bacillus subtilis spores gave decreasing amounts of pyrimidine dimers and increasing amounts of spore photoproduct as the sasp/dna ratio was increased. the yields of pyrimidine dimers and spore photoproduct were less than 0.2% and 8% of total thymine, respectively, when dna saturated with sasp was irradiated at 254 nm with 30 kj/m2; in the absence of sasp the yields were reversed-4.5% and 0.3%, resp ...19911924287
pericarditis due to bacillus cereus in an intravenous drug user. 19911925304
comparative study of various hydrogen ion buffers to assay zn(2+)-dependent beta-lactamases.the low zn2+ complex formation constants, the capacity to degrade penicillin g in combination with zn2+, and uv absorbance make 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 3-[n-tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid, 1,3-bis[tris(hydroxymethyl)methylamino]propane, and 1,4-piperazinediethanesulfonic acid convenient buffers to study zn(2+)-dependent beta-lactamases.19911929321
interaction of beta-lactamases i and ii from bacillus cereus with semisynthetic cephamycins. kinetic studies.the influence of c-6 alpha- or c-7 alpha-methoxylation of the beta-lactam ring in the catalytic action of class a and b beta-lactamases has been investigated. for this purpose the kinetic behaviour of beta-lactamases i (class a) and ii (class b) from bacillus cereus was analysed by using several cephamycins, moxalactam, temocillin and related antibiotics. these compounds behaved as poor substrates for beta-lactamase ii, with high km values and very low catalytic efficiencies. in the case of beta ...19911930129
mechanism of acyl transfer by the class a serine beta-lactamase of streptomyces albus g.optimization by energy minimization of stable complexes occurring along the pathway of hydrolysis of benzylpenicillin and cephalosporin c by the streptomyces albus g beta-lactamase has highlighted a proton shuttle that may explain the catalytic mechanism of the beta-lactamases of class a. five residues, s70, s130, n132, t235 and a237, are involved in ligand binding. the gamma-oh group of t235 and, in the case of benzylpenicillin, the gamma-oh group of s130 interact with the carboxylate group, on ...19911930139
metabolism of nitric oxide in denitrifying pseudomonas aeruginosa and nitrate-respiring bacillus cereus.suspensions of pseudomonas aeruginosa and bacillus cereus were continuously sparged with nitrogen to remove gaseous products of nitrate reduction. under these conditions, p. aeruginosa denitrified nitrate to 4% no, 47% n2o and 49% n2. b. cereus reduced nitrate to 94% nitrite, 2% no and 5% n2o. b. cereus was unable to reduce no or n2o as sole electron acceptor, whereas p. aeruginosa reduced no stoichiometrically to n2o when the n2o reductase was inhibited by acetylene or when the formed n2o was i ...19911936933
kinetics of the hydrolysis of monodispersed and micellar phosphatidylcholines catalyzed by a phospholipase c from bacillus cereus.the phosphatidylcholine-hydrolyzing phospholipase c, so-called "phospholipase c" (plc), was isolated from the culture of bacillus cereus strain iam 1208. the amino-acid composition and partial n-terminal sequence of the purified enzyme were in good agreement with those expected from the nucleotide sequence for a plc of strain atcc 10987 [johansen et al. (1988) gene 65, 293-304]. the chain-length dependence of kinetic parameters for the plc-catalyzed hydrolysis of monodispersed short-chain phosph ...19911939031
metabolism of 17 beta-estradiol by oral streptococcus mutans, streptococcus sanguis, bacillus cereus and candida albicans.the ability of oral isolates of streptococcus mutans, streptococcus sanguis, bacillus cereus and candida albicans to metabolize 17 beta-estradiol was analyzed by thin-layer chromatography using 4-14c-estradiol as the substrate. all test microorganisms metabolized 17 beta-estradiol. estrone was the main metabolic product, except for b. cereus, which converted 17 beta-estradiol mainly to unidentified, more polar metabolites. s. sanguis and b. cereus had the most active metabolism but s. mutans and ...19911945489
purification and some characteristics of a calcium-binding protein from bacillus cereus spores.a novel calcium-binding protein has been purified from the dormant spores of bacillus cereus t. purity of this protein was verified by sds-page and reversed-phase hplc. its calcium-binding ability was verified by a competitive calcium-binding assay using chelex-100 resin and 45ca autoradiography. the protein is heat-stable and is retained by hydrophobic matrices (phenyl-sepharose) in a calcium-dependent manner. sds-page and amino acid composition indicate the molecular mass of the protein to be ...19911955855
preparation and application of an affinity matrix for phosphatidylinositol-specific phospholipase c.a non-hydrolyzable phosphonate analogue of phosphatidyl inositol, racemic myo-inosityl-(1)-5-oxa-16-trifluoroacetamidohexadecyl phosphonate, was synthesized. this phosphonate inhibited the activity of phosphatidyl inositol-specific phospholipase c (pi-plc) from bacillus cereus with an ic50 of approximately 10 mm. removal of the trifluoroacetyl blocking group followed by covalent binding of the phosphonate to cyanogen bromide activated sepharose 4b via the amino group produced an affinity matrix ...19901965711
identification of the reactive cysteinyl residue and atp binding site in bacillus cereus glutamine synthetase by chemical modification.bacillus cereus glutamine synthetase was modified by reaction with a fluorescent sh reagent, n-[[(iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonic acid (iaedans), or an atp analog, 5'-p-fluorosulfonylbenzoyladenosine (fsba). the locations of the specific binding sites of these reagents were identified. iaedans inactivated mg2(+)-dependent activity and activated mn2(+)-dependent activity. fsba inactivated only mn2(+)-dependent activity. mg2+ plus mn2(+)-dependent activity was inactivated by iae ...19901972941
purification and characterization of n-acetylmuramoyl-l-alanine amidase from human serum.purification to homogeneity of the n-acetylmuramoyl-l-alanine amidase (mucopeptide amidohydrolase, ec from human serum has been achieved with a high yield. by molecular sieving chromatography, a molecular weight of 120,000-130,000 has been found for the native enzyme. polyacrylamide gel electrophoresis under native conditions gave a unique band of mr = 125,000. the same technique performed under denaturing conditions revealed that the protein is a dimer composed of one subunit of mr = ...19901974148
site-directed mutagenesis of beta-lactamase i. single and double mutants of glu-166 and lys-73.two single mutants and the corresponding double mutant of beta-lactamase i from bacillus cereus 569/h were constructed and their kinetics investigated. the mutants have lys-73 replaced by arginine (k73r), or glu-166 replaced by aspartic acid (e166d), or both (k73r + e166d). all four rate constants in the acyl-enzyme mechanism were determined for the e166d mutant by the methods described by christensen, martin & waley [(1990) biochem. j. 266, 853-861]. both the rate constants for acylation and de ...19901980064
residues of doxycycline and oxytetracycline in eggs after medication via drinking water to laying hens.doxycycline (dotc) and oxytetracycline (otc) were dissolved in drinking water (0.5 g/l) and supplied to laying hens for 7 consecutive days. eggs laid were collected daily during and after medication, and the antibiotic concentrations in the yolk and albumin were determined by the cup-plate method with bacillus cereus var. mycoides atcc 11778. the concentrations of both antibiotics were increased in yolk day by day with the advance in medication, reached peaks 2 days after withdrawal and then dec ...19912015932
effects of high hydrostatic pressure on characteristics of pork slurries and inactivation of microorganisms associated with meat and meat products.pork slurries inoculated with various test microorganisms were prepared and subjected to high hydrostatic pressure at 1000 to 6000 atm for 10 min at 25 degrees c to examine for the pressure effects on characteristics of the slurries and the inactivation of the microorganisms associated with meat and meat products. pressure treatment at higher than 3000 atm caused coagulation and discoloration of the pork slurries. harder and more white coagulants were obtained by increasing the pressure. pressur ...19912049285
kinetic analysis of dehydropeptidase-i and comparative in vitro and in vivo stabilities of ps-5 derivatives modified at the c-3 side chain.with partially purified kidney dehydropeptidase-i (dhp-i) preparations, the hydrolysis kinetics of glycyldehydrophenylalanine (gly-dph) by dhp-i were found to be completely non-michaelian, whereas those of ps-5 and imipenem were composed of at least two-phase reactions which were also observed using bacillus cereus type ii beta-lactamase. thus the dhp-i stabilities of 34 ps-5 carbapenem derivatives which were synthesized by chemical modification at the c-3 side chain of ps-5 were examined in vit ...19912054858
transduction of certain genes by an autonomously replicating bacillus thuringiensis phage.a derivative of bacillus thuringiensis subsp. kurstaki hd1 (hd1-9) released transducing phage (tp21) from late exponential cultures. three of seven markers tested were transduced into bacillus cereus, but only two of these (cysc and trpb/f) were transduced at a frequency of more than 100 times the reversion rates. a limited transduction capacity was given further support in that few chromosomal markers were carried in the hd1-9 lysate, as demonstrated by southern hybridization. restriction fragm ...19912059027
toxin production by bacillus pumilus.two strains of bacillus pumilus (m11 and m38) and one strain each of bacillus cereus (m27), bacillus subtilis (m67), and enterobacter agglomerans (m14) were identified from the air of lancashire cotton mills. these strains were tested for cytopathic effects in vero cells; b pumilus and b cereus strains were also examined for haemolytic activity, lecithinase production, and proteolytic action on casein. rounding and clumping of the vero cells occurred after the addition of supernatants prepared f ...19912066422
dead salmonellae or their endotoxin accelerate the early course of a salmonella infection in mice.the course of a salmonella infection following a low intravenous dose of virulent organisms was studied in mice. simultaneous administration of 10(4) s. typhimurium c5 together with c. 10(8) dead salmonellae caused a marked acceleration of early net bacterial growth in the liver and spleen, leading to a rapidly overwhelming infection. administration of similar numbers of either staphylococcus albus or bacillus cereus had no effect, whereas 20 micrograms of s. typhimurium boivin-type lipopolysacc ...19902087155
effect of phospholipase c from bacillus cereus on the release of membrane-bound choline-o-acetyltransferase from rat hippocampal tissue.some of the enzyme choline-o-acetyltransferase (chat) associated with central cholinergic nerve terminals appears to be non-ionically associated with membranes. in the present study, we tested the possibility that some membrane-bound chat might be anchored to membranes by a phosphatidylinositol linkage by incubating rat hippocampal tissue with phospholipase c (plc) from bacillus cereus. the plc selectively augmented the release of chat; also, the glycosylphosphatidylinositol-plc inhibitor, zinc, ...19902106007
bacillus-induced endophthalmitis: new series of 10 cases and review of the literature.we reviewed the charts of 10 patients who were admitted to the massachusetts eye and ear infirmary over a 10-year period with the diagnosis of bacillus species endophthalmitis. to our knowledge this is the largest single series in the literature and includes the first two reported cases of bacillus endophthalmitis following glaucoma filtering procedures. seven cases developed following penetrating ocular trauma. one occurred in an intravenous drug abuser. five eyes ultimately underwent enucleati ...19902106339
inhibition of phosphatidylinositol-specific phospholipase c by phosphonate substrate analogues.non-hydrolysable analogues of phosphatidylinositol were synthesized and tested as inhibitors of phosphatidylinositol-specific phospholipase c from bacillus cereus. in these molecules, the phosphodiester bond of phosphatidylinositol hydrolyzed by the phospholipase was replaced by a phosphonate linkage and a simpler hydrophobic group replaced the diacylglycerol moiety. one of the phosphonates also contained a carboxylate functional group suitable for matrix attachment. all three synthetic phosphon ...19902106349
bacteriological quality of on-farm manufactured goat cheese.the bacteriological quality of 198 ripened soft or semi-soft goat cheeses obtained from dairy farms and the retail trade was investigated. the cheeses were examined for total counts of aerobic bacteria, coliform bacteria (37 and 44 degrees c respectively), enterococci, coagulase positive staphylococci, bacillus cereus and clostridium perfringens. cheeses obtained from dairy-farms were also determined for ph value. in terms of all tests performed, cheeses made of heat-treated milk with starter cu ...19902106443
identification of bacillus anthracis by using monoclonal antibody to cell wall galactose-n-acetylglucosamine polysaccharide.guanidine extracts of crude bacillus anthracis cell wall were used to vaccinate balb/c mice and to develop monoclonal antibody (mab) to vegetative cell surface antigens. two hybridomas selected during this study produced immunoglobulin m immunoglobulins, which appear to be directed to an epitope associated with the galactose-n-acetyl-d-glucosamine polysaccharide. both demonstrated specificity in their binding to purified b. anthracis cell wall, o-stearoyl-polysaccharide conjugates, and intact, n ...19902107201
release of dialkylglycerol from purple membrane phospholipids by phospholipase d.when the major polar lipid of purple membrane, a dialkyl analogue of phosphatidyl glycerophosphate, is treated with phospholipase d under the usual assay conditions for this enzyme, the reaction yields dialkylglycerol and glycerol bisphosphate, i.e. the kind of products that would be expected from a phospholipase c reaction. the effect is seen both in native purple membranes and with the pure phospholipid in the form of liposomes. the specific activity and kinetic parameters km and vmax of phosp ...19902108151
detective work in continuous ambulatory peritoneal dialysis.we report five cases of continuous ambulatory peritoneal dialysis in which the mechanisms and sources of infection were established. we show how diligent enquiry and environmental investigation can explain the pathogenesis of infection and help in prevention by motivation of the patient.19902108222
factors influencing optimization of diffusion assays for antibiotics.agar nutrient content, cylinder charge volume, thickness (volume) of the agar layer, and incubation temperature were 4 factors varied to determine their effect(s) on the optimization of the cylinder-plate diffusion assay. chlortetracycline was the pilot antibiotic and bacillus cereus was used as the assay organism. zones of inhibition were larger when the incubation temperature was lower than that which was commonly used and/or when the nutrient level was decreased; the zones were smaller when t ...19902108955
nucleotide sequence of the tetracycline resistance gene of pbc16 from bacillus cereus. 19902109312
a second species of bacillus causing primary cutaneous disease. 19902109735
purification of phospholipase-c from bacillus cereus by affinity chromatography on 2-(4-aminophenylsulphonyl)ethyl-cellulose.a new method for the purification of phospholipase-c (phosphatidylcholine cholinephosphohydrolase, ec from bacillus cereus has been developed, based on its affinity to 2-(4-aminophenylsulphonyl)ethyl derivative of beaded cellulose. the enzyme was adsorbed on the affinity sorbent through a site(s) that was clearly distinct from its catalytically active site, because it was still active in the immobilized state. a possible role of enzyme-inhibitor interaction in enzyme binding to the liga ...19902109761
sequencing the gene for an imipenem-cefoxitin-hydrolyzing enzyme (cfia) from bacteroides fragilis tal2480 reveals strong similarity between cfia and bacillus cereus beta-lactamase ii.using a newly constructed bacteroides fragilis-escherichia coli cloning shuttle vector, pjst61, we have cloned the cefoxitin (fox)-imipenem (imp) resistance determinant from b. fragilis tal2480. fox-imp resistance in this strain results from the production of a periplasmic, zn2(+)-containing beta-lactamase which hydrolyzes carbapenems and cephamycins and whose activity is resistant to clavulanic acid but sensitive to zn2(+)-binding reagents, including edta. the pjst61 vector permits efficient li ...19902110145
[study on the discrimination of bacteria by gas chromatographic profiles of cellular monosaccharides].a procedure for obtaining gas chromatographic (gc) profiles of bacterial cellular monosaccharides was described. some of the unknown component peaks in these profiles were identified. and, based on the complete linkage cluster analysis with the euclidean distance coefficient, the interpretation of the resulting cellular monosaccharides of bacteria were performed by mini-computer. by means of this method, the discrimination of 5 species (24 strains) of aerobic endospore-forming bacteria. the resu ...19902111604
[characteristics of kinetics of phospholipid hydrolysis by phospholipase c from bacillus cereus. hydrolysis of phosphatidylcholine in the presence of deoxycholate].using dynamic light scattering and 31p-nmr spectroscopy methods, the reaction of solubilization of phosphatidylcholine by the ionic detergent, sodium deoxycholate, in aqueous solutions was studied. the kinetics of phosphatidylchodine hydrolysis by phospholipase c from b. cereus depending on the size and structural organization of substrate aggregates was investigated. no phosphatidylcholine hydrolysis was observed in the case of lamellar organization of the substrate, the size of lamellas not ex ...19902111715
[dynamics of accumulation of extracellular lipoteichoic acid in bacillus cereus].the dynamic of accumulation of extracellular lipoteichoic acid (lta) was studied depending on the growth stage of bacillus cereus st. 96. a maximum amount of extracellular lta was detected in the middle of the exponential growth. the quantity of the biopolymer present in the culture medium at the beginning of the stationary growth under conditions of catabolite repression of sporulation and without repression was found to be different. experimentally increased concentrations of lta inhibited b. ...19902112246
phospholipase c-mediated hydrolysis of phosphatidylcholine is an important step in pdgf-stimulated dna synthesis.recent evidence suggests the involvement of phosphatidylcholine (pc) hydrolysis both in the control of normal cell growth and in transformation. we show here that the simple exogenous addition of bacillus cereus pc-hydrolyzing phospholipase c (pc-plc) is sufficient to elicit a potent mitogenic response in swiss 3t3 fibroblasts by a mechanism that is independent of protein kinase c. our results on the additivity and synergism between b. cereus pc-plc, pdgf, and insulin in the mitogenic response i ...19902112426
studies on the cellular defense reactions of the madeira cockroach, leucophaea maderae: in vitro phagocytosis of different strains of bacillus cereus and their effect on hemocyte viability.monolayers of leucophaea maderae hemocytes, consisting of mainly plasmatocytes and coagulocytes, were incubated with three strains of bacillus cereus of differing pathogenicities, and the levels of phagocytosis and hemocyte viability were determined. incubation with viable b. cereus strains nctc 2599, ncib 3329, and b1 resulted in a significant drop in hemocyte viability after 60 min of incubation compared with the saline-only controls. the greatest effect, however, resulted from incubation with ...19902112578
n-acetylmuramic acid inhibits spore germination and germination enzymes.this report describes the inhibition of spore germination by n-acetylmuramic acid. at concentrations of 20 mm and higher, the rate of germination of the treated spores was ten-fold lower than that of the control spores. the inhibitor also depressed the activity of two germination-related enzymes; a coat-associated hexosaminidase and a core enzyme. these findings suggests that n-acetylmuramic acid affects spore germination by inhibition of the corticolytic enzymes. the results are discussed with ...19902112602
enzymatic synthesis of purine 2'-deoxyriboside and its properties as an inhibitor of adenosine deaminases from calf intestinal mucosa and bacillus cereus.enzymatic synthesis of purine 2'-deoxyriboside was obtained by reacting purine with excess 2-deoxy-alpha-d-ribose-1-phosphate in the presence of commercial bovine nucleoside phosphorylase; the product was isolated by semipreparative reverse phase hplc with an overall 62% yield. purine 2'-deoxyriboside was shown to behave as a competitive inhibitor of adenosine deaminase from calf intestinal mucosa and bacillus cereus, with apparent ki values of 4.5 and 8.5 microm, respectively.19902113897
[the genetic transfer, heredity and phenotypic expression of plasmid rp4::mu cts genes in bacillus cereus].the transcipients were obtained in intrageneric matings of e.coli donor harbouring the plasmid pr4::mu cts 62 with bac. cereus gp7 recipient cells with the frequency 10(-9). the transcipient clone bac. cereus 682 was selected having stably inherited and expressed the hybrid plasmid pr4::mu cts 62 genes for antibiotic resistance and temperature sensitivity. production of the bacteriophage mu cts 62 particles was not registered in the bacillary transcipient cells. the plasmid rp4::mu cts 62 genes ...19902114185
a novel bicomponent hemolysin from bacillus cereus.a procedure combining isoelectric focusing (sephadex ief) and fast protein liquid chromatography (superose 12; mono-q) removed hemolytic activity (presumably a contaminant) from partially purified preparations of the multicomponent diarrheal enterotoxin produced by bacillus cereus. however, when the separated fractions were recombined, hemolytic activity was restored, suggesting that hemolysis is a property of the enterotoxin components. combined fractions exhibited a unique ring pattern in gel ...19902114359
acp broadsheet 124: may 1990. examination of faeces for bacterial pathogens. 19902115050
complete visual recovery after bacillus cereus endophthalmitis in a child. 19902116090
production of poly-(beta-hydroxybutyric-co-beta-hydroxyvaleric) acids.alcaligenes latus, alcaligenes eutrophus, bacillus cereus, pseudomonas pseudoflava, pseudomonas cepacia, and micrococcus halodenitrificans were found to accumulate poly-(beta-hydroxybutyric-co-beta-hydroxyvaleric) acid [p(hb-co-hv)] copolymer when supplied with glucose (or sucrose in the case of a. latus) and propionic acid under nitrogen-limited conditions. a fed-batch culture of a. eutrophus produced 24 g of poly-beta-hydroxybutyric acid (phb) liter-1 under ammonium limitation conditions. when ...19902117877
the blasticidin s resistance gene (bsr) selectable in a single copy state in the bacillus subtilis chromosome.the blasticidin s resistance gene (bsr), originally isolated from bacillus cereus, was studied in bacillus subtilis. it was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer bs resistance on b. subtilis when integrated in its chromosome, even in a single copy state. the construction of a bsr gene cassette and its practical application as a novel selection marker for b. subtilis are reported.19902118136
selection and application of antibodies modifying the function of beta-lactamase.nine monoclonal antibodies directed against class a beta-lactamases were detected and selected by a novel screening procedure based on assaying the modifications in the catalytic and stability properties of beta-lactamase in solution. unlike conventional screening, e.g., elisa or immunoprecipitation, the present method does not depend on firm binding and thus favors detection of low affinity antibodies. individual antibodies were found to affect the enzymatic activity in various ways including s ...19902118387
survival of pathogenic microorganisms in an egg-nog-like product containing 7% ethanol.a liquor consisting of whole egg, saccharose (25% w/v) and ethanol (7.0% w/v) was artificially contaminated with salmonella enteritidis, s. typhimurium, staphylococcus aureus (three different strains), bacillus cereus and listeria monocytogenes. after 3 weeks of incubation at 22 degrees c the numbers of salmonella, s. aureus and l. monocytogenes decreased more than 3 log10 units. under such conditions, however, the total number of microorganisms increased 3 log10 units. at 4 degrees c the decrea ...19902118795
microbiological quality of ayib, a traditional ethiopian cottage samples of ayib, a traditional ethiopian cottage cheese, were purchased from awassa market and analysed for their aerobic mesophilic counts, psychrotrophic counts, yeasts and molds, coliforms, spore-formers, enterococci, lactic acid bacteria. listeria monocytogenes, staphylococci and bacillus cereus. the majority of the samples showed counts of mesophilic aerobic bacteria, yeasts and enterococci of 10(8), 10(7) and 10(7) cfu/g. about 55% of the samples were positive for coliforms and ...19902118797
psychrotrophic strains of bacillus cereus producing investigations on three outbreaks of bacillus cereus food poisoning in spain and the netherlands, the causative strains grew within a temperature range of 4-37 degrees c, but not at 43 degrees c. such psychrotrophic types were found to occur in various dairy products (including ca 25% of 35 samples of pasteurized milk) and some mousses and cook/chill meals. growth of and enterotoxin production by psychrotrophic b. cereus could be prevented by temperatures below 4 degrees c and ph-values not e ...19902118898
synthesis of 3-aryl-2-substituted-4(3h)-quinazolines as potential antimicrobial agents.three new series of 4(3h)-quinazolinone derivatives were synthesized, namely: [3-aryl-4(3h)-quinazolinon-2-yl]-methylenehydrazino-(n- substituted)-thiocarbamides, 3-aryl-2-(3-substituted-4-phenyl-2,3-dihydrothiazol-2-ylidenehy drazonomethyl)-4 (3h)-quinazolinones and 3-aryl-2-(3-substituted-4-oxothiazolidin-2-ylidenehydrazonometh yl)-4(3h)-quinazolinones. the antimicrobial activities of the synthesized compounds were also studied.19902119178
analytical methods for bacillus cereus and other bacillus species.bacillus cereus can give rise to two distinct forms of foodborne disease, the emetic and the diarrhoeal syndromes. the emetic syndrome is believed to be associated with an emetic toxin pre-formed in food. cooked rice is the most common vehicle, and the symptoms are similar to those of staphylococcus aureus intoxication. the diarrhoeal type is caused by an enterotoxin and the symptoms generally parallel those of the clostridium perfringens food poisoning. the heat resistance of b. cereus spores a ...19902119209
plasmid-associated sensitivity of bacillus thuringiensis to uv light.spores and vegetative cells of bacillus thuringiensis were more sensitive to uv light than were spores or cells of plasmid-cured b. thuringiensis strains or of the closely related bacillus cereus. introduction of b. thuringiensis plasmids into b. cereus by cell mating increased the uv sensitivity of the cells and spores. protoxins encoded by one or more b. thuringiensis plasmids were not involved in spore sensitivity, since a b. thuringiensis strain conditional for protoxin accumulation was equa ...19902119568
primary structure of the oligo-1,6-glucosidase of bacillus cereus atcc7064 deduced from the nucleotide sequence of the cloned gene.the gene coding for bacillus cereus atcc7064 (mesophile) oligo-1,6-glucosidase was cloned within a 2.8-kb sali-ecori fragment of dna, using the plasmid puc19 as a vector and escherichia coli c600 as a host. e. coli c600 bearing the hybrid plasmid pbce4 accumulated oligo-1,6-glucosidase in the cytoplasm. the cloned enzyme coincided absolutely with b. cereus oligo-1,6-glucosidase in its mr (65,000), in its electrophoretic behavior on a polyacrylamide gel with or without sodium dodecyl sulfate, in ...19902120057
the bactericidal activity of a deuteroporphyrin-hemin mixture on gram-positive bacteria. a microbiological and spectroscopic study.the combined antibacterial activity of various porphyrins with hemin on gram-positive bacteria was studied. protoporphyrin, hematoporphyrin derivative and deuteroporphyrin show only a marginal inhibitory effect in the dark. however, hemin has a strong cytotoxic effect which is independent of illumination and is equally strong in the dark. the disadvantage of hemin treatment is that it is temporary. in this study, we have demonstrated that a combination of deuteroporphyrin and hemin has a unique ...19902120409
effect of disulphite on protein and pyridine-2,6-dicarboxylic acid synthesis in sporulating cells of bacterial species.the effect of disulphite on protein and pyridine-2,6-dicarboxylic acid (dipicolinic acid, dpa) synthesis was investigated in sporulating cells of bacillus subtilis e52 and b. cereus w18. progressive reductions were evident in the protein and dpa concentrations for both sporulating cells with increasing concentrations (100 to 600 micrograms ml-1) of disulphite. a significant (p less than 0.05) reduction in protein synthesis by disulphite was exhibited, culminating in a decrease in protein synthes ...19902120550
cloning and sequencing of the class b beta-lactamase gene (ccra) from bacteroides fragilis tal3636.bacteroides fragilis tal3636 produces a class b, zn2(+)-requiring beta-lactamase. the gene, ccra, was cloned and expressed in escherichia coli. the gene was sequenced and shown to share greater than 33% identity with the metalloenzyme from bacillus cereus 569/h.19902121094
the effect of glucose, starch, and ph on growth, enterotoxin and haemolysin production by strains of bacillus cereus associated with food poisoning and non-gastrointestinal infection.brain-heart infusion medium, modified by (a) 1.0% w/v glucose supplement, (b) 1.0% w/v soluble starch supplement, (c) ph adjustment to 8.8 or (d) ph adjustment to 5.0, was used to investigate the influence of glucose, starch and ph on growth, enterotoxin and haemolysin production by bacillus cereus. the four test strains selected for comparison originated from episodes of emetic-syndrome food poisoning, diarrhoeal-syndrome food poisoning, traumatic wound infection and bovine mastitis, respective ...19902121214
effect of metabisulphite on sporulation and alkaline phosphatase in bacillus subtilis and bacillus cereus.the effect of metabisulphite on spore formation and alkaline phosphatase activity/production in bacillus subtilis and bacillus cereus was investigated both in liquid and semi-solid substrates. while supplementary nutrient broth (snb) and sporulation medium (sm) were used as the liquid growth media, two brands of powdered milk were used as the food (semi-solid) substrates. under both aerobic and anaerobic conditions, b. subtilis was more resistant to metabisulphite than b. cereus while the level ...19902122190
a medium for the isolation, enumeration and rapid presumptive identification of injured clostridium perfringens and bacillus cereus.a blood-free egg yolk medium (bcp) containing pyruvate, inositol, mannitol and a bromocresol purple indicator in a nutrient agar base has been developed to initiate the growth of clostridium perfringens. it is comparable to blood agar for the growth of normal, chilled stored vegetative cells and heat-injured spores of cl. perfringens and bacillus cereus. it has the advantage over blood agar in exhibiting presumptive evidence of cl. perfringens (production of lecithinase and inositol fermentation ...19902123173
effects of heat-, cacl2- and ethanol-treatments on activation of bacillus spores.the effects of heat, cacl2, and ethanol on activation of bacillus spores were determined by monitoring the absorbance decrease during germination in inosine. bacillus cereus t, b. subtilis a and b. megaterium qm b1551 spores were activated by heat- and cacl2-treatments. ethanol activated b. megaterium and b. subtilis spores yet did not activate b. cereus spores. cacl2- and ethanol-activations were less effective than heat-activation as judged by optimal germination rates and germination extents. ...19902123174
evidence for a role of phosphatidylcholine-hydrolysing phospholipase c in the regulation of protein kinase c by ras and src oncogenes.the products of ras and src oncogenes are thought to be important components in pathways regulating cell proliferation and differentiation. in fibroblasts transformed by these oncogenes, increased diacylglycerol levels have been found which most probably arise from activation of the turnover of phosphatidylcholine. diacylglycerol is a key activator of protein kinase c whose role in cell growth and transformation has been proposed. we demonstrate here by using immunochemical techniques that trans ...19902123453
sodium hypophosphite inhibition of the growth of selected gram-positive foodborne pathogenic bacteria.sodium hypophosphite (shp) was evaluated for inhibition of growth of selected gram-positive foodborne pathogenic bacteria in trypticase soy broth. in addition, the effects of ph and sodium chloride (nacl) alone and in combination with (shp) were also examined. all inhibition studies were performed with optimal or nearly optimal growth conditions for each bacterium. growth was monitored by determining culture optical density at 600 nm, and a time to significant growth determined for each test med ...19902124497
[intensity of hemolysin and lecithinase production by bacillus cereus strains isolated from food].the study was carried out on the filtrates of cultures of 60 bacillus cereus strains isolated from food products which caused food poisoning (20 strains), from patients (20 strains) and from food analysed in routine supervision. the strains isolated from food poisoning demonstrated a more pronounced haemolytic activity as compared with the strains from routine supervision. a lack of correlation was demonstrated between the intensity of lecithinase production and the intensity of reaction of rabb ...19902125144
[the effect of respiratory toxins on the bacterial concentration of trivalent gold].it has been found that concentration of trivalent gold by bacillus cereus v-4368 cells is sensitive to respiratory inhibitors such as rotenone and antimycin a. inhibitory action of rotenone is neutralized by succinate, that of antimycin--by atp. atp influence disappears in presence of dccd. it is supposed that au(iii) concentration is due to the atpase action and the respiration systems supply this process with atp.19902125381
detection of common flagella antigen in bacillus cereus by monoclonal antibody.bacillus cereus has been classified into 23 types by immunochemical analyses of flagella antigen, but a common antigenic determinant of flagella had not been determined. when the immunochemical method of classification had changed from "agglutination method" to "enzyme-linked immunosorbent assay (elisa)," the cross-reactivity between each flagella type was increased. these results indicated that the application of elisa method would enable detection of the common antigenic determinant of b. cere ...19902126335
plasmid transformation of bacillus cereus on cellophane membranes.a simple approach to test the ability of bacteria to undergo natural genetic transformation is suggested. the basic feature of the approach is the cultivation of bacterial cells in the presence of exogenous (plasmid) dna on cellophane membranes placed successively on nutrient and selective agar. using this approach the ability of bacillus cereus for "natural" genetic transformation was detected. transformation frequencies varied from 10(-8) to 10(-6).19902126512
forces involved in adhesion of bacillus cereus spores to solid surfaces under different environmental conditions.the adhesion of bacillus cereus spores (nctc 2599) to hydrophobic and hydrophilic glass surfaces was studied when environmental conditions were varied. the spores were exposed in media of different polarities as well as different ph and ionic concentrations. with increasing ethanol concentrations, the polarity of the medium was decreased and the predominant force of attraction was found to be hydrophobic. the spore surface was uncharged at a ph around 3, at which value the spore was most adhesiv ...19902127266
in vitro activity of sparfloxacin (ci-978; at-4140) against clinical isolates from cancer patients.the in vitro activity of sparfloxacin, a new quinolone, was compared with those of ciprofloxacin and fleroxacin against gram-positive and gram-negative bacteria, greater than 90% of which were isolated from blood culture specimens of cancer patients. sparfloxacin was extremely active against acinetobacter species, aeromonas hydrophila, citrobacter diversus, enterobacter species, escherichia coli, klebsiella species, proteus vulgaris, and serratia marcescens (inhibiting greater than 90% of these ...19902127348
mercury resistance determined by a self-transmissible plasmid in bacillus cereus 5.inducible mercuric reductase activity in bacillus cereus 5 was plasmid-encoded. plasmid analysis revealed three plasmids with molecular masses of 2.6, 5.2 and 130 mda. a mating system permitted transfer of the resistance determinant among strains of b. cereus and b. thuringiensis. transfer of mercury resistance from b. cereus 5 to b. cereus 569 and b. thuringiensis occurred during mixed culture incubation on agar surfaces. the 130-mda plasmid (pgb130) was responsible for transfer; frequencies ra ...19902127369
secondary structure of sphingomyelinase from bacillus cereus.of the total of 306 amino acids in the sequence of sphingomyelinase (smplc) from bacillus cereus, almost half (150) are expected to be involved in the formation of loop or turn structure, while 65 and 73 residues may participate in the formation of alpha-helix and beta-structure, respectively. the helix content of smplc was calculated to be 0-5%, based on the cd spectra. the addition of divalent metal ions such as mg2+ or both ca2+ and mg2+ had no effect on the cd spectra of smplc, although the ...19902127932
metabolism of progesterone and testosterone by bacillus cereus strain socransky 67 and streptococcus mutans strain ingbritt.bacillus cereus strain socransky 67 and streptococcus mutans strain ingbritt were grown overnight in complex medium and then in fresh medium flasks for 5 h. the bacterial cells in the medium were centrifuged and resuspended; 4-14c-progesterone or 4-14c-testosterone was added, and the samples were incubated for 2 h at 37 degrees c in a shaking water bath. the metabolites were analyzed with column and thin layer chromatography and radioautography and quantified by liquid scintillation counting. on ...19902127957
degranulation of human neutrophils after exposure to bacterial phospholipase c.because an endogenous phospholipase c (plc) participates in neutrophil activation and because many bacterial pathogens produce plcs, these studies examined the effect of plc from bacillus cereus on the release of the granule enzyme lysozyme from human neutrophils. bacillus cereus plc caused dose-dependent lysozyme release, and combined stimulation of neutrophils with plc and fluoride led to increased secretion. stimulation of neutrophil degranulation is a potential contributing factor for tissue ...19902128277
[sensitivity to antibiotics and selected compounds of bacillus cereus isolated from food products and cases of food poisoning].the study was done on 60 strains of bacillus cereus isolated from cases of food poisoning and from food products tested in current supervision. the highest sensitivity was found to the antibiotics: streptomycin, neomycin, gentamicin, amikacin, oxytetracycline, and sulphonamides: negram and sulfathiazole . all strains were resistant to colistin and cloxacillin, nearly all were resistant to penicillin, ampicillin and optochine.19902129276
asymmetric distribution of the phosphatidylinositol-linked phospho-oligosaccharide that mimics insulin action in the plasma membrane.we have investigated the topography of a glycosyl-phosphatidylinositol implicated in insulin action by a combination of two complementary methods: (a) chemical labelling with a non-permeable (isethionyl acetimidate) and a permeable (ethyl acetimidate) probe; and (b) enzymatic modifications with beta-galactosidase (ec or phosphatidylinositol-specific phospholipase c (ec using the first approach the majority of the glycosyl-phosphatidylinositol is found in the outer surface of ...19902138537
identification and characterization of glycosylphosphatidylinositol-linked schistosoma mansoni adult worm immunogens.metabolic radiolabeling of adult worms of schistosoma mansoni with [3h]myristic acid has revealed that the fatty acid is incorporated into more than 15 proteins. we have shown that two of these proteins, a 200-kda glycoprotein known to be exposed on the surface of the adult worm following praziquantel treatment and a 22-kda glycoprotein that shows an enhanced immune reactivity with sera of vaccinated mice, are anchored to the adult worm membrane via a glycosylphosphatidylinositol (gpi) linkage. ...19902139172
in vitro activity of ly264826, a new glycopeptide antibiotic, against gram-positive bacteria isolated from patients with cancer.the in vitro activity of ly264826, a novel glycopeptide antibiotic produced by amycolatopsis orientalis, was compared with those of vancomycin, teicoplanin, and oxacillin against 311 gram-positive clinical isolates from patients with cancer, ly264826 had lower mics for 90% of isolates (mic90) than vancomycin for all species tested. it was active against oxacillin-resistant isolates including staphylococcus aureus (mic90, 0.5 micrograms/ml), staphylococcus haemolyticus (mic90, 2.0 micrograms/ml), ...19902149921
[the effect of glutaraldehyde on mycobacteria and on selected types of bacteria].the author tested the action of 2% alkalinized glutaraldehyde on mycobacterium tuberculosis, m. kansasii, m. avium, m. xenopi and m. fortuitum, as well as staphyloccocus aureus, escherichia coli, pseudomonas aeruginosa, proteus vulgaris, bacillus cereus, b. megatherium and b. subtilis. after 5-minute exposure devitalization of m. tuberculosis, m. kansasii and vegetative forms of the tested bacteria occurred. a sporicide effect was recorded already after 30 minutes or after more than 120 minutes. ...19902150610
[products of bacillus cereus contributing to its pathogenicity].in a review the author describes the most important exoproducts of strains of bacillus cereus which contribute to its pathogenicity. the toxic product are evaluated with regard to their physical, chemical and biological properties as well with regard to mechanism of their pathogenic action.19902150614
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