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bacillus cereus ar156 activates pamp-triggered immunity and induces a systemic acquired resistance through a npr1-and sa-dependent signaling pathway.induced resistance responses play a potent role in plant defense system against pathogen attack. bacillus cereus ar156 is a plant growth promoting rhizobacterium (pgpr) that installs induced systemic resistance (isr) to pseudomonas syringae pv. tomato (pst) in arabidopsis. here, we show that ar156 leaf infiltration enhances disease resistance in arabidopsis through the activation of a systemic acquired resistance (sar). pr1 protein expression and reactive oxygen species (ros) burst are strongly ...201626616055
microgravity-driven remodeling of the proteome reveals insights into molecular mechanisms and signal networks involved in response to the space flight environment.space is a hostile environment characterized by high vacuum, extreme temperatures, meteoroids, space debris, ionospheric plasma, microgravity and space radiation, which all represent risks for human health. a deep understanding of the biological consequences of exposure to the space environment is required to design efficient countermeasures to minimize their negative impact on human health. recently, proteomic approaches have received a significant amount of attention in the effort to further s ...201626571091
dual-recognition detection of staphylococcus aureus using vancomycin-functionalized magnetic beads as concentration carriers.vancomycin, which has a strong antibacterial effect to gram-positive bacteria, was adopted as one molecular recognition agent for bacterial detection. magnetic beads (mbs) were functionalized with this antibiotic to effectively concentrate staphylococcus aureus (s. aureus). in addition, alkaline phosphatase (alp)-tagged rabbit immunoglobulin g (alp-igg) was used as the second recognition agent to improve the specificity based on the binding between the fc region of rabbit igg and protein a in th ...201626606309
production of rhamnolipids and diesel oil degradation by bacteria isolated from soil contaminated by petroleum.biosurfactants are microbial secondary metabolites. the most studied are rhamnolipids, which decrease the surface tension and have emulsifying capacity. in this study, the production of biosurfactants, with emphasis on rhamnolipids, and diesel oil degradation by 18 strains of bacteria isolated from waste landfill soil contaminated by petroleum was analyzed. among the studied bacteria, gram-positive endospore forming rods (39%), gram positive rods without endospores (17%), and gram-negative rods ...201626588432
detection of viable enterotoxin-producing bacillus cereus and analysis of toxigenicity from ready-to-eat foods and infant formula milk powder by multiplex pcr.bacillus cereus is responsible for several outbreaks of foodborne diseases due to its emetic toxin and enterotoxin. enterotoxins, cytotoxin k (cytk), nonhemolytic enterotoxin (nhe), and hemolysin bl (hbl), have been recorded in several diarrheal cases due to food poisoning from b. cereus. the objective of this study was to develop a rapid and accurate method that combines multiplex pcr with propidium monoazide to selectively detect viable cells of enterotoxin-producing b. cereus in milk powder, ...201626686715
bacillus cereus infection: 57 case patients and a literature review.we aimed to study the characteristics of patients presenting with a bacillus cereus infection in a university hospital.201626525185
production of co-polymers of polyhydroxyalkanoates by regulating the hydrolysis of biowastes.production of polyhydroxyalkanoate (pha) co-polymers by bacillus spp. was studied by feeding defined volatile fatty acids (vfas) obtained through controlled hydrolysis of various wastes. eleven mixed hydrolytic cultures (mhcs) each containing 6 strains could generate vfa from slurries of (2% total solids): pea-shells (ps), potato peels (pp), apple pomace (ap) and onion peels (op). ps hydrolysates (obtained with mhc2 and mhc5) inoculated with bacillus cereus egu43 and bacillus thuringiensis egu45 ...201626512866
role of plant growth promoting rhizobacteria and ag-nano particle in the bioremediation of heavy metals and maize growth under municipal wastewater irrigation.the investigation evaluated the role of plant growth promoting rhizobacteria (pgpr) and ag-nano particle on the growth and metabolism of maize irrigated with municipal wastewater (mw). three pgpr isolated from mw were identified on the basis of 16s-rrna gene sequence analyses as pseudomonas sp., pseudomonas fluorescence, and bacillus cereus. the municipal waste water was used to irrigate the maize seeds inoculated with 3 isolated pgpr. the isolated pgpr had catalase and oxidase enzymes, solubili ...201626507686
phytochemical, sub-acute toxicity, and antibacterial evaluation of cordia sebestena leaf extracts.in nigeria, cordia sebestena (boraginaceae), an understudied medicinal plant, is used in traditional medicine for the treatment of gastrointestinal disorders. in this study, we investigated the chemical composition, antibacterial potential, and sub-acute toxicity of c. sebestena leaves.201626501159
bioavailability and mobility of organic contaminants in soil: new three-step ecotoxicological evaluation.a novel approach was developed for rapid assessment of bioavailability and potential mobility of contaminants in soil. the response of the same test organism to the organic extract, water extract and solid phase of soil was recorded and compared. this approach was designed to give an initial estimate of the total organic toxicity (response to organic extractable fraction), as well as the mobile (response to water extract) and bioavailable fraction (response to solid phase) of soil samples. eight ...201626490898
inactivation of bacterial pathogens in yoba mutandabota, a dairy product fermented with the probiotic lactobacillus rhamnosus yoba.mutandabota is a dairy product consumed as a major source of proteins and micronutrients in southern africa. in this study the microbial safety of traditional and a variant of mutandabota fermented with the probiotic lactobacillus rhamnosus yoba (yoba mutandabota) was investigated by challenging the products with five important food pathogens: listeria monocytogenes, salmonella spp., campylobacter jejuni, escherichia coli o157:h7 and bacillus cereus. pasteurized full-fat cow's milk was used for ...201626490648
antioedematogenic activity, acetylcholinesterase inhibition and antimicrobial properties of jacaranda oxyphylla.jacaranda oxyphylla cham. (bignoniaceae) is a shrub found in the brazilian cerrado and used in folk medicine to treat microbial infections. the aim of this study was to carry out a phytochemical screening and evaluate antioedematogenic, antimicrobial and antiacetylcholinesterase properties of j. oxyphylla crude extracts. all extracts analysed showed presence of terpenoids, which are potentially active chemical substances. a high ache inhibitory activity for hexane extract from leaves and for the ...201626469996
biomineralization of pb(ii) into pb-hydroxyapatite induced by bacillus cereus 12-2 isolated from lead-zinc mine tailings.the remediation of pb(ii) through biomineralization is rergarded as a promising technique as well as an interesting phenomenon for transforming heavy metals from mobile species into very stable minerals in the environment. studies are well needed for in-depth understanding the mechanism of pb(ii) immobilized by bacteria. in the present study, we investigated the uptake and biomineralization of pb(ii) using bacillus cereus 12-2 isolated from lead-zinc mine tailings. the maximum pb(ii) uptake capa ...201626468754
characterization of microfouling and corrosive bacterial community of a firewater distribution system.this investigation provides generic information on the culturable corrosive and the microfouling bacterial community in a firewater distribution system that uses freshwater. conventional microbiological methods were used for the selective isolation of the major microfouling bacteria. the isolates were characterized by 16s rrna gene sequencing and the biofilm as well as the corrosion characteristics of the isolates were evaluated. pseudomonas aeruginosa and bacillus cereus were predominantly obse ...201626467696
rapid enhanced photocatalytic degradation of dyes using novel n-doped zro2.a novel n-doped zro2 (n-zro2) photocatalyst is synthesized through thermal decomposition of zirconium hydroxide-urea complex and is characterized using various techniques, including xrd, ftir, tga, sem, tem, uv-drs, xps, xanes, and bet. the n-zro2 possesses pure monoclinic structure with high crystallinity. by using the proposed facile route of synthesis, both interstitial and substitutional n doping with high dopant stability can be realized. the optical properties of the catalyst are significa ...201626439860
recent research progress with phospholipase c from bacillus cereus.phospholipase c (plc) catalyzes the hydrolysis of phospholipids to produce phosphate monoesters and diacylglycerol. it has many applications in the enzymatic degumming of plant oils. plc bc , a bacterial plc from bacillus cereus, is an optimal choice for this activity in terms of its wide substrate spectrum, high activity, and approved safety. unfortunately, its large-scale production and reliable high-throughput screening of plc bc remain challenging. herein, we summarize the research progress ...201626437973
transcription factors wrky70 and wrky11 served as regulators in rhizobacterium bacillus cereus ar156-induced systemic resistance to pseudomonas syringae pv. tomato dc3000 in arabidopsis.the activation of both the sa and ja/etsignalling pathways may lead to more efficient general and broad resistance to pst dc3000 by non-pathogenic rhizobacteria. however, the mechanisms that govern this simultaneous activation are unclear. using arabidopsis as a model system, two transcription factors, wrky11 and wrky70, were identified as important regulators involved in induced systemic resistance (isr) triggered by bacillus cereus ar156. the results revealed that ar156 treatment significantly ...201626433201
single-step purification of chitosanases from bacillus cereus using expanded bed chromatography.a chitosanase-producing strain was isolated and identified as bacillus cereus c-01. the purification and characterization of two chitosanases were studied. the purification assay was accomplished by ion exchange expanded-bed chromatography. experiments were carried out in the presence and in the absence of cells through different expansion degree to evaluate the process performance. the adsorption experiments demonstrated that the biomass does not affect substantially the adsorption capacity of ...201626433174
molecular characterisation of bacillus chitinase for bioconversion of chitin waste.in this work chitin was extracted chemically from shrimp shells. seventeen bacillus isolates were screened for chitinolytic activity. the chitinolytic strains of bt. were screened at different temperatures and phs for their hydrolytic potentials. by using a pair of specific primers, endochitinase gene was amplified from sbs bt-5 strain through pcr, and then cloned into ptz57 ta cloning vector and transferred in escherichia coli dh5α strain. the sequenced gene (genbank accession no: he995800) con ...201626373305
antimicrobial susceptibility and the in vitro postantibiotic effects of vancomycin and ciprofloxacin against bacillus cereus isolates.vancomycin and ciprofloxacin were often used in the therapy of infections associated with bacillus cereus.201626358183
diversity of bacterial communities in the midgut of bactrocera cucurbitae (diptera: tephritidae) populations and their potential use as attractants.the microbiota plays an important role in insect development and fitness. understanding the gut microbiota composition is essential for the development of pest management strategies. midgut bacteria were isolated from nine wild b. cucurbitae populations collected from different agroecological zones of india. these isolates were further studied for attractant potential of fruit fly adults, and the chemical constituents in the supernatants of gut bacteria were analysed.201626299539
synthesis of novel sulfonamide analogs containing sulfamerazine/sulfaguanidine and their biological activities.sulfamerazine and sulfaguanidine are clenched with p-nitrobenzoyl chloride and the products obtained are reduced to naxs in ethanol-water. novel sulfonamides (6a-g and 9a-g) were synthesized by the reaction of these reduced products (4 and 8) with various sulfonyl chlorides (5a-g). the structures of these compounds were characterized using spectroscopic analysis (ir, (1)h-nmr, (13)c-nmr and hrms) technique. antimicrobial activity of sulfonamides (3, 4, 7, 8, 6a-g and 9a-g) was evaluated by the a ...201626327456
inactivation of bacillus cereus spores in a tsuyu sauce using continuous ohmic heating with five sequential elbow-type electrodes.the effect of ohmic heating (oh) in a pilot plant system which had a zig-zag shaped (elbow-type) ohmic heater with five sequential voltage electrodes was investigated on bacillus cereus spores in a commercial tsuyu sauce.201626497155
antiproliferative and antibacterial activity of some glutarimide derivatives.antiproliferative and antibacterial activities of nine glutarimide derivatives (1-9) were reported. cytotoxicity of compounds was tested toward three human cancer cell lines, hela, k562 and mda-mb-453 by mtt assay. compound 7 (2-benzyl-2-azaspiro[5.11]heptadecane-1,3,7-trione), containing 12-membered ketone ring, was found to be the most potent toward all tested cell lines (ic50 = 9-27 μm). preliminary screening of antibacterial activity by a disk diffusion method showed that gram-positive bacte ...201626247353
response of indigenously developed bacterial consortia in progressive degradation of polyvinyl chloride.thermoplastic-based materials are recalcitrant in nature, which extensive use affect environmental health. here, we attempt to compare the response of indigenously produced bacterial consortium-i and consortium-ii in degrading polyvinyl chloride (pvc). these consortia were developed by using different combination of bacterial strains of pseudomonas otitidis, bacillus cereus, and acanthopleurobacter pedis from waste disposal sites of northern india after their identification via 16s rdna sequenci ...201626231814
noninvasive ph monitoring for bacterial detection in platelet concentrates.bacterial contamination of platelet concentrates (pcs) remains the prevalent posttransfusion infectious risk. the ph safe system, a noninvasive method used to measure ph of pc for quality control, was evaluated herein as a rapid method to detect bacterial contamination in pcs.201627028108
antibacterial properties and effects of fruit chilling and extract storage on antioxidant activity, total phenolic and anthocyanin content of four date palm (phoenix dactylifera) cultivars.phoenix dactylifera or date palm fruits are reported to contain natural compounds that exhibit antioxidant and antibacterial properties. this research aimed to study the effect of fruit chilling at 4 °c for 8 weeks, extract storage at -20 °c for 5 weeks, and extraction solvents (methanol or acetone) on total phenolic content (tpc), antioxidant activity and antibacterial properties of saudi arabian p. dactylifera cv mabroom, safawi and ajwa, as well as iranian p. dactylifera cv mariami. the stora ...201627023514
optimization of peptide nucleic acid fluorescence in situ hybridization (pna-fish) for the detection of bacteria: the effect of ph, dextran sulfate and probe concentration.fluorescence in situ hybridization (fish) is a molecular technique widely used for the detection and characterization of microbial populations. fish is affected by a wide variety of abiotic and biotic variables and the way they interact with each other. this is translated into a wide variability of fish procedures found in the literature. the aim of this work is to systematically study the effects of ph, dextran sulfate and probe concentration in the fish protocol, using a general peptide nuclei ...201627021959
adhesion and removal kinetics of bacillus cereus biofilms on ni-ptfe modified stainless steel.biofilm control remains a challenge to food safety. a well-studied non-fouling coating involves codeposition of polytetrafluoroethylene (ptfe) during electroless plating. this coating has been reported to reduce foulant build-up during pasteurization, but opportunities remain in demonstrating its efficacy in inhibiting biofilm formation. herein, the initial adhesion, biofilm formation, and removal kinetics of bacillus cereus on ni-ptfe-modified stainless steel (ss) are characterized. coatings lo ...201627020838
erratum to: seasonal trend and clinical presentation of bacillus cereus bloodstream infection: association with summer and indwelling catheter.bacillus cereus, an opportunistic pathogen, can cause fatal infection. however, b. cereus bloodstream infections (bsis) have not been well characterised. from 2008 to 2013, b. cereus isolates from all of the specimens and patients with b. cereus bsis were identified. environmental samples were collected to detect b. cereus contamination. we also characterised the clinical presentation of b. cereus bsi through analyses of risk factors for bsi and mortality. a total of 143 clinical b. cereus isola ...201627010814
identification of cereulide-producing bacillus cereus by nucleic acid chromatography and reverse transcription real-time pcr.rna extracts were analyzed with a nucleic acid sequence-based amplification (nasba) - nucleic acid chromatography and a reverse transcription-quantitative pcr assay (rt-qpcr) based on the taqman probe for identification of cereulide-producing bacillus cereus. all 100 emetic b. cereus strains were found to give positive results, but 50 diarrheal b. cereus strains and other bacterial species showed negative results in the nasba-chromatography. that is, the assay could selectively identify the emet ...201627009509
antibacterial activity and action mechanism of the essential oil from enteromorpha linza l. against foodborne pathogenic bacteria.foodborne illness and disease caused by foodborne pathogenic bacteria is continuing to increase day by day and it has become an important topic of concern among various food industries. many types of synthetic antibacterial agents have been used in food processing and food preservation; however, they are not safe and have resulted in various health-related issues. therefore, in the present study, essential oil from an edible seaweed, enteromorpha linza (aeo), was evaluated for its antibacterial ...201627007365
synthesis and evaluation of ester derivatives of 10-hydroxycanthin-6-one as potential antimicrobial agents.as part of our continuing research on canthin-6-one antimicrobial agents, a new series of ester derivatives of 10-hydroxycanthin-6-one were synthesized using a simple and effective synthetic route. the structure of each compound was characterized by nmr, esi-ms, ft-ir, uv, and elemental analysis. the antimicrobial activity of these compounds against three phytopathogenic fungi (alternaria solani, fusarium graminearum, and fusarium solani) and four bacteria (bacillus cereus, bacillus subtilis, ra ...201627007362
phytochemical analysis, antimicrobial and antioxidant activities of euphorbia golondrina l.c. wheeler (euphorbiaceae juss.): an unexplored medicinal herb reported from cameroon.this study aimed at determining the phytochemical constituents of euphorbia golondrina l.c. wheeler, an alien invasive medicinal herb that is used for the treatment of gastroenteritis related ailments, diabetes, conjunctivitis, gastritis, enterocolitis, tonsillitis, vaginitis, hemorrhoids, prostatism, warts and painful swellings by the mundani people of the mount bambouto caldera in southwestern cameroon, and to evaluate its in vitro antimicrobial and antioxidant activities. susceptibility testi ...201627006873
secreted compounds of the probiotic bacillus clausii strain o/c inhibit the cytotoxic effects induced by clostridium difficile and bacillus cereus toxins.although the use of probiotics based on bacillus strains to fight off intestinal pathogens and antibiotic-associated diarrhea is widespread, the mechanisms involved in producing their beneficial effects remain unclear. here, we studied the ability of compounds secreted by the probiotic bacillus clausii strain o/c to counteract the cytotoxic effects induced by toxins of two pathogens, clostridium difficile and bacillus cereus, by evaluating eukaryotic cell viability and expression of selected gen ...201627001810
evaluation of bacterial contamination of dental unit waterlines and use of a newly designed measurement device to assess retraction of a dental chair unit.dental unit waterline (duwl) output water is delivered through instruments of a dental chair unit (dcu) to irrigate and cool teeth. however, these waterlines can be heavily contaminated with bacteria.201627000421
shelf life of donkey milk subjected to different treatment and storage conditions.the aim of this study was to investigate the effects of different treatment conditions on microbiological indicators of donkey milk hygiene and their evolution during shelf life at 4 and 12°c from 3 to 30d, simulating a farm-scale pasteurization and packing system. four treatment conditions were tested: no treatment (raw milk), pasteurization (65°c × 30 min), high-pressure processing (hpp), and pasteurization plus hpp. the microbiological quality of the raw donkey milk investigated was not optim ...201626995124
purification and characterization of plantaricin jla-9: a novel bacteriocin against bacillus spp. produced by lactobacillus plantarum jla-9 from suan-tsai, a traditional chinese fermented cabbage.bacteriocins are ribosomally synthesized peptides with antimicrobial activity produced by numerous bacteria. a novel bacteriocin-producing strain, lactobacillus plantarum jla-9, isolated from suan-tsai, a traditional chinese fermented cabbage, was screened and identified by its physiobiochemical characteristics and 16s rdna sequence analysis. a new bacteriocin, designated plantaricin jla-9, was purified using butanol extraction, gel filtration, and reverse-phase high-performance liquid chromatog ...201626985692
formation and biopharmaceutical characterization of electrospun pvp mats with propolis and silver nanoparticles for fast releasing wound dressing.antibacterial, antiviral, antifungal, antioxidant, anti-inflammatory, and anticancer activities of propolis and its ability to stimulate the immune system and promote wound healing make it a proper component for wound dressing materials. silver nanoparticles are recognized to demonstrate strong antiseptic and antimicrobial activity; thus, it also could be considered in the development of products for wound healing. combining propolis and silver nanoparticles can result in improved characteristic ...201626981531
assessment of the bacterial contamination of hand air dryer in washrooms.the present study was carried out, using standard techniques, to identify and count the bacterial contamination of hand air dryers, used in washrooms. bacteria were isolated from the air flow, outlet nozzle of warm air dryers in fifteen air dryers used in these washrooms. bacteria were found to be relatively numerous in the air flows. bacterially contaminated air was found to be emitted whenever a warm air dryer was running, even when not being used for hand drying. our investigation shows that ...201626981009
development of a double-antibody sandwich elisa for rapid detection of bacillus cereus in food.bacillus cereus is increasingly recognized as one of the major causes of food poisoning in the industrialized world. in this paper, we describe a sensitive double-antibody sandwich enzyme-linked immunosorbent assay (elisa) that was developed for rapid detection of b. cereus in food to minimize the risk of contamination. the polyclonal antibody (pab) and monoclonal antibodies (mabs) specific to b. cereus were generated from rabbit antiserum and mouse ascites, respectively, using the octanoic acid ...201626976753
recombinant broad-range phospholipase c from listeria monocytogenes exhibits optimal activity at acidic ph.the broad-range phospholipase c (plc) from listeria monocytogenes has been expressed using an intein expression system and characterized. this zinc metalloenzyme, similar to the homologous enzyme from bacillus cereus, targets a wide range of lipid substrates. with monomeric substrates, the length of the hydrophobic acyl chain has significant impact on enzyme efficiency by affecting substrate affinity (km). based on a homology model of the enzyme to the b. cereus protein, several active site resi ...201626976751
recombinant thiopeptides containing noncanonical amino acids.thiopeptides are a subclass of ribosomally synthesized and posttranslationally modified peptides (ripps) with complex molecular architectures and an array of biological activities, including potent antimicrobial activity. here we report the generation of thiopeptides containing noncanonical amino acids (ncaas) by introducing orthogonal amber suppressor aminoacyl-trna synthetase/trna pairs into a thiocillin producer strain of bacillus cereus .we demonstrate that thiopeptide variants containing nc ...201626976568
application of mathematical models for bacterial inactivation curves using hypericin-based photosensitization.the aim of this study was to evaluate which of the three mathematical models (weibull, geeraerd and logistic) makes the best approximation for hypericin-based photosensitization bacterial inactivation.201626972682
new coumarin derivatives from ferula pseudalliacea with antibacterial activity.in this study, the antibacterial activity of disesquiterpene coumarin and sesquiterpene coumarins obtained from ferula pseudalliacea roots was evaluated by determination of minimum inhibitory concentration using the broth micro-dilution method against seven pathogenic bacterial strains (staphylococcus aureus atcc 25,923, vancomycin resistant clinical strain of enterococcus faecium, bacillus cereus ptcc 1015, escherichia coli atcc 25,922, pseudomonas aeruginosa ptcc 1430, clinical strain of klebs ...201626953526
four new carbazole alkaloids from murraya koenigii that display anti-inflammatory and anti-microbial activities.in our present study, four new, designated as murrayakonine a-d (), along with 18 known carbazole alkaloids were isolated from chcl3 : meoh (1 : 1) crude extracts of the stems and leaves of murraya koenigii (linn.) spreng. the structures of the all isolated compounds were characterized by analysis of hr-esi-ms and nmr (1d and 2d spectroscopy) results, and comparison of their data with the literature data. for the first time, all the isolates were evaluated for their anti-inflammatory activities, ...201626947457
the effect of storage temperature and duration on the microbial quality of bulk tank milk.the dairy industry in ireland is currently undergoing a period of expansion and, as a result, it is anticipated that milk may be stored in bulk tanks on-farm for periods greater than 48 h. the objective of this study was to investigate the effects of storage temperature and duration on microbial quality of bulk tank milk when fresh milk is added to the bulk tank twice daily. bulk tank milk stored at 3 temperatures was sampled at 24-h intervals during storage periods of 0 to 96 h. bulk tank milk ...201626947309
complete genome sequence of the cold-active bacteriophage vmy22 from bacillus cereus.the cold-active bacteriophage vmy22, belonging to the podoviridae family, was isolated from mingyong glacier in china. sequence analysis revealed that the genome is 18,609 bp long, with an overall g + c content of 36.4 mol%, and 25 open reading frames (orfs). the sequence contains 46 potential promoters, 6 transcription terminators, and no trnas. most of the orfs show a high degree of similarity to b103 (nc_004165). two noteworthy findings were made. first, one of the predicted proteins, orf 19, ...201626941234
a new benzopyrans derivatives from a mangrove-derived fungus penicillium citrinum from the south china sea.one new benzopyran derivative (2r(*),4r(*))-3,4-dihydro-5-methoxy-2-methyl-2h-1-benzopyran-4-ol (1), together with five known compounds (2-6), were obtained from the etoac extract of the endophytic fungus penicillium citrinum hl-5126 isolated from the mangrove brguiera sexangula var. rhynchopetala collected in the south china sea. their structures were elucidated by the detailed analysis of comprehensive spectroscopic data. all compounds were evaluated for their antibacterial activities. compoun ...201626930107
eco-friendly and facile integrated biological-cum-photo assisted electrooxidation process for degradation of textile wastewater.the present article reports an integrated treatment method viz biodegradation followed by photo-assisted electrooxidation, as a new approach, for the abatement of textile wastewater. in the first stage of the integrated treatment scheme, the chemical oxygen demand (cod) of the real textile effluent was reduced by a biodegradation process using hydrogels of cellulose-degrading bacillus cereus. the bio-treated effluent was then subjected to the second stage of the integrated scheme viz indirect el ...201626921849
isolation and structural elucidation of brevibacillin, an antimicrobial lipopeptide from brevibacillus laterosporus that combats drug-resistant gram-positive bacteria.a new environmental bacterial strain exhibited strong antimicrobial characteristics against methicillin-resistant staphylococcus aureus, vancomycin-resistant strains of enterococcus faecalis and lactobacillus plantarum, and other gram-positive bacteria. the producer strain, designated osy-i1, was determined to be brevibacillus laterosporusvia morphological, biochemical, and genetic analyses. the antimicrobial agent was extracted from cells of osy-i1 with isopropanol, purified by high-performance ...201626921428
use of peripheral parenteral nutrition solutions as a risk factor for bacillus cereus peripheral venous catheter-associated bloodstream infection at a japanese tertiary care hospital: a case-control study.the risk factors are unclear for peripheral venous catheter-associated bloodstream infections (pvcbsis) caused by bacillus cereus. we aimed to examine for these risk factors in patients with b. cereus pvcbsi by conducting a 2-year case-control study in a large teaching hospital. we analyzed all adult cases of b. cereus pvcbsi (37 patients) and 180 controls who were randomly selected from among patients who had a pvc in place for at least 2 days. multivariate analysis using a conditional logistic ...201626902219
a rapid and simple real-time pcr assay for detecting foodborne pathogenic bacteria in human feces.a rapid, simple method for detecting foodborne pathogenic bacteria in human feces is greatly needed. here, we examined the efficacy of a method that employs a combination of a commercial pcr master mix, which is insensitive to pcr inhibitors, and a dna extraction method which used sodium dodecyl benzene sulfonate (sdbs), and tween 20 to counteract the inhibitory effects of sdbs on the pcr assay. this method could detect the target genes (stx1 and stx2 of enterohemorrhagic escherichia coli, inva ...201626902211
surface modification of a polyethylene film for anticoagulant and anti-microbial catheter.a functional anticoagulant and anti-bacterial coating for polyethylene (pe) films is described. the stepwise preparation of this nanocomposite surface coating involves o2 plasma etching of pe film, carbodiimide coupling of cysteamine to the etched pe film, binding of ag to sulfhydryl groups of cysteamine, and assembly of heparin capped agnps on the pe film. the nanocomposite film and its components were characterized by (1)h-nuclear magnetic resonance spectroscopy, attenuated total reflectance-f ...201626900340
fabrication of an electrochemical dna-based biosensor for bacillus cereus detection in milk and infant formula.this paper describes fabrication of a dna-based au-nanoparticle modified pencil graphite electrode (pge) biosensor for detection of bacillus cereus, causative agent of two types of food-borne disease, i.e., emetic and diarrheal syndrome. the sensing element of the biosensor was comprised of gold nanoparticles (gnps) self-assembled with single-stranded dna (ssdna) of nhea gene immobilized with thiol linker on the gnps modified pge. the size, shape and dispersion of the gnps were characterized by ...201626896793
bacillus licheniformis trehalose-6-phosphate hydrolase structures suggest keys to substrate specificity.trehalose-6-phosphate hydrolase (trea) belongs to glycoside hydrolase family 13 (gh13) and catalyzes the hydrolysis of trehalose 6-phosphate (t6p) to yield glucose and glucose 6-phosphate. the products of this reaction can be further metabolized by the energy-generating glycolytic pathway. here, crystal structures of bacillus licheniformis trea (bltrea) and its r201q mutant complexed with p-nitrophenyl-α-d-glucopyranoside (r201q-ppng) are presented at 2.0 and 2.05 å resolution, respectively. the ...201626894535
bacteria-based polythene degradation products: gc-ms analysis and toxicity testing.polythene degradation leads to the production of various by-products depending upon the type of degradation process. the polythene degradation products (pedp) in the culture supernatant of the two bacteria (lysinibacillus fusiformis strain vasb14/wl and bacillus cereus strain vasb1/ts) were analyzed with gc-ms technique. the major by-products in the pedp in the culture supernatant of l. fusiformis strain vasb14/wl (1,2,3,4 tetra methyl benzene) and b. cereus strain vasb1/ts (1,2,3 trimethyl benz ...201626888528
association of genotyping of bacillus cereus with clinical features of post-traumatic endophthalmitis.bacillus cereus is the second most frequent cause of post-traumatic bacterial endophthalmitis. although genotyping of b. cereus associated with gastrointestinal infections has been reported, little is known about the b. cereus clinical isolates associated with post-traumatic endophthalmitis. this is largely due to the limited number of clinical strains available isolated from infected tissues of patients with post-traumatic endophthalmitis. in this study, we report successful isolation of twenty ...201626886446
investigation of equilibrium and kinetics of cr(vi) adsorption by dried bacillus cereus using response surface methodology.in this study, response surface methodology (rsm) based on three-variable-five-level central composite rotatable design was used to analyze the effects of combined and individual operating parameters (biomass dose, initial concentration of cr(vi) and ph) on the cr(vi) adsorption capacity of dried bacillus cereus. a quadratic polynomial equation was obtained to predict the adsorbed cr(vi) amount. analysis of variance showed that the effect of biomass dose was the key factor in the removal of cr(v ...201626877045
the pulsed light inactivation of veterinary relevant microbial biofilms and the use of a rtpcr assay to detect parasite species within biofilm structures.the presence of pathogenic organisms namely parasite species and bacteria in biofilms in veterinary settings, is a public health concern in relation to human and animal exposure. veterinary clinics represent a significant risk factor for the transfer of pathogens from housed animals to humans, especially in cases of wound infection and the shedding of faecal matter. this study aims to provide a means of detecting veterinary relevant parasite species in bacterial biofilms, and to provide a means ...201626862516
uptake and levels of the antibiotic berberine in individual dormant and germinating clostridium difficile and bacillus cereus spores as measured by laser tweezers raman spectroscopy.spores of clostridium difficile and bacillus cereus are major causes of nosocomial diarrhoea and foodborne disease. our aim was to measure the dynamics of the uptake of the antibiotic berberine by individual germinating spores and the levels of berberine accumulated in germinated spores.201626861569
antioxidant and dna damage protecting activity of exopolysaccharides from the endophytic bacterium bacillus cereus sz1.an endophytic bacterium was isolated from the chinese medicinal plant artemisia annua l. the phylogenetic and physiological characterization indicated that the isolate, strain sz-1, was bacillus cereus. the endophyte could produce an exopolysaccharide (eps) at 46 mg/l. the 1,1-diphenyl-2-picrylhydracyl (dpph) radical scavenging activity of the eps reached more than 50% at 3-5 mg/ml. the eps was also effective in scavenging superoxide radical in a concentration dependent fashion with an ec50 valu ...201626861269
understanding the antimicrobial activity behind thin- and thick-rolled copper plates.the aim of this study was to compare the antibacterial properties of the surfaces of copper plates that were rolled to a thickness of 25 and 100 μm. differences in topology of 25- and 100-μm-thick copper plates were studied using scanning electron microscopy (sem), atomic force microscopy (afm), and x-ray diffraction (xrd). antibacterial activity of the copper surfaces was tested against strains of staphylococcus aureus, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, listeria m ...201626860943
biological production of silver nanoparticles by soil isolated bacteria and preliminary study of their cytotoxicity and cutaneous wound healing efficiency in rat.biosynthesis of agnps by 37 different bacterial soil isolates was done and confirmed through visible spectrophotometry. fifteen isolates were identified and two of them with the highest ability of agnps production were used for transmission electron microscopy (tem) and x-ray diffraction (xrd) tests. mtt assay for both of the obtained agnps was run and after determination their ic50s, two different toxic and nontoxic doses of each agnps solution were chosen for wound healing assay. forty eight r ...201626854241
effect of fermented broth from lactic acid bacteria on pathogenic bacteria proliferation.in this study, the effect that 5 fermented broths of lactic acid bacteria (lab) strains have on the viability or proliferation and adhesion of 7 potentially pathogenic microorganisms was tested. the fermented broth from lactococcus lactis c660 had a growth inhibitory effect on escherichia coli k92 that reached of 31%, 19% to pseudomonas fluorescens, and 76% to staphylococcus epidermidis. the growth of staph. epidermidis was negatively affected to 90% by lc. lactis 11454 broth, whereas the growth ...201626851857
detection and quantification of viable bacillus cereus group species in milk by propidium monoazide quantitative real-time pcr.the bacillus cereus group includes important spore-forming bacteria that present spoilage capability and may cause foodborne diseases. these microorganisms are traditionally evaluated in food using culturing methods, which can be laborious and time-consuming, and may also fail to detect bacteria in a viable but nonculturable state. the purpose of this study was to develop a quantitative real-time pcr (qpcr) combined with a propidium monoazide (pma) treatment to analyze the contamination of uht m ...201626830746
antagonistics against pathogenic bacillus cereus in milk fermentation by lactobacillus plantarum zdy2013 and its anti-adhesion effect on caco-2 cells against pathogens.lactobacillus plantarum zdy2013 is a potential probiotic isolated from fermented bean acid. in this study, we aimed to evaluate the in vitro antimicrobial activity of this organism against bacillus cereus in milk fermentation, the antiadhesion ability on intestinal epithelial cells, as well as its ability to abrogate the cytotoxic effect and expression levels of genes. we found no antimicrobial activity produced by l. plantarum once the ph was adjusted to 6.0 and 7.0. the ph decreased continuous ...201626830743
isolation and characterization of spore-forming bacilli (sfb) from shepherd's purse (capsella bursa-pastoris).shepherd's purse (capsella bursa-pastoris), native to europe, is commonly consumed fresh and sometimes inadequately washed before consumption in korea. the objective of this study was to characterize isolates of spore-forming bacilli (sfb) in samples of fresh shepherd's purse. three genera were identified: bacillus (9 species), paenibacillus (3 species), and brevibacillus (1 species). none of the genes of the hemolysin bl (hbl) and nonhemolytic enterotoxin (nhe) complexes, or of the emetic toxin ...201626822957
antibacterial and synergistic activity of pentacyclic triterpenoids isolated from alstonia scholaris.(1) background: alstonia scholaris (apocynaceae) is an important medicinal plant that has been historically used in "dai" ethnopharmacy to treat infectious diseases in china. although various pharmacological activities have been reported, the antimicrobial constitutes of a. scholaris have not yet been identified. the objective of this study is to evaluate the antibacterial constitutes from the leaf extract of a. scholaris and to assess the synergistic effects of isolated compounds with antibioti ...201626821000
in vitro antibacterial activity of selected medicinal plants traditionally used in vietnam against human pathogenic bacteria.medicinal plants are widely used for the treatment of different infectious diseases. infectious diseases caused by bacteria have a large impact on public health. this study aimed to determine the in vitro antibacterial activity of the medicinal plants traditionally used in vietnam against the bacterial strains associated with infectious diseases.201626819218
characterization and exposure assessment of emetic bacillus cereus and cereulide production in food products on the dutch market.the emetic toxin cereulide, which can be produced by bacillus cereus, can be the cause of food poisoning upon ingestion by the consumer. the toxin causes vomiting and is mainly produced in farinaceous food products. this article includes the prevalence of b. cereus and of cereulide in food products in the netherlands, a characterization of b. cereus isolates obtained, cereulide production conditions, and a comparison of consumer exposure estimates with those of a previous exposure assessment. fo ...201626818983
antimicrobial compounds from endophytic streptomyces sp. bcc72023 isolated from rice (oryza sativa l.).an endophytic actinomycete strain bcc72023 was isolated from rice (oryza sativa l.) and identified as the genus streptomyces, based on phenotypic, chemotaxonomic and 16s rrna gene sequence analyses. the strain showed 99.80% similarity compared with streptomyces samsunensis m1463(t). chemical investigation led to the isolation of three macrolides, efomycins m (1), g (2) and oxohygrolidin (3), along with two polyethers, abierixin (4) and 29-o-methylabierixin (5). to our knowledge, this is the firs ...201626809052
evaluation of probiotic properties of lactobacillus plantarum wlpl04 isolated from human breast milk.lactobacillus plantarum wlpl04, a specific strain isolated from human breast milk, was investigated for its survival capacity (acid and bile salt tolerance, survival in simulated gastrointestinal tract, inhibition of pathogens, antibiotic susceptibility, yield of exopolysaccharides) and probiotic properties (antiadhesion of pathogens, protection from harmful effect of sodium dodecyl sulfate, and antiinflammatory stress on caco-2 cells). the results showed that lb. plantarum wlpl04 had broad-spec ...201626805974
characterization of antimicrobial lipopeptides produced by bacillus sp. lm7 isolated from chungkookjang, a korean traditional fermented soybean food.a wild-type microorganism exhibiting antimicrobial activities was isolated from the korean traditional fermented soybean food chungkookjang and identified as bacillus sp. lm7. during its stationary growth phase, the microorganism secreted an antimicrobial substance, which we partially purified using a simple two-step procedure involving ammonium sulfate precipitation and heat treatment. the partially purified antimicrobial substance, anti-lm7, was stable over a broad ph range (4.0-9.0) and at te ...201626803269
diversity of sugar acceptor of glycosyltransferase 1 from bacillus cereus and its application for glucoside synthesis.glycosyltransferase 1 from bacillus cereus (bcgt1) catalyzes the transfer of a glucosyl moiety from uridine diphosphate glucose (udp-glucose) to various acceptors; it was expressed and characterized. the specificity of acceptors was found to be broad: more than 20 compounds classified into o-, s-, and n-linkage glucosides can be prepared with bcgt1 catalysis. based on this work, we conclude that the corresponding acceptors of these compounds must possess the following features: (1) the acceptors ...201626795959
α,β-dibromochalcone derivatives--synthesis and antimicrobial activity.to obtain some chalcones and their dibrominated analogues and to evaluate their antimicrobial potential.201626793867
microbiome associations in pigs with the best and worst clinical outcomes following co-infection with porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2).on a world-wide basis, co-infections involving porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2) are common and contribute to a range of polymicrobial disease syndromes in swine. both viruses compromise host defenses, resulting in increased susceptibility to infections by primary and secondary pathogens that can affect growth performance as well as increased morbidity and mortality. an experimental population of 95 pigs was co-infected with prrsv an ...201627139023
preparation and biological activity of new collagen composites, part i: collagen/zinc titanate nanocomposites.the aim of this investigation was to develop new antimicrobial collagen/zinc titanate (zntio3) biomaterials using a sol-gel cryogenic draying technology in keeping the native collagen activity. broad-spectrum antimicrobial activity was demonstrated against firmicutes (staphylococcus epidermidis, bacillus cereus, and candida lusitaniae) and gracilicutes (escherichia coli, salmonella enterica, and pseudomonas putida) microorganisms. the antimicrobial activity as well as the cytotoxicity were speci ...201627138724
structural and biochemical characterization of the bacillus cereus 3-hydroxyisobutyrate dehydrogenase.the 3-hydroxyisobutyrate dehydrogenase (hibadh) family catalyzes the nad(+)- or nadp(+)-dependent oxidation of various β-hydroxyacid substrates into their cognate semialdehydes for diverse metabolic pathways. because hibadh group members exhibit different substrate specificities, the substrate-recognition mode of each enzyme should be individually characterized. in the current study, we report the biochemical and structural analysis of a hibadh group enzyme from bacillus cereus (bchibadh). bchib ...201627120461
relapsing peritonitis with bacillus cereus in a patient on continuous ambulatory peritoneal dialysis.we present a case where bacillus cereus was determined to be the causative agent of relapsing peritonitis in a patient on continuous ambulatory peritoneal dialysis (capd). the patient, a 70-year-old man from the faroe islands, was admitted with relapsing peritonitis four times over a 3-month period. peritoneal cultures were positive for growth of b. cereus, a rare bacterial cause of peritonitis. the cultures demonstrated susceptibility to vancomycin, and therefore the patient was treated with in ...201627118739
culture-dependent and -independent methods to investigate the predominant microorganisms associated with wet processed coffee.the fermentation process of yunnan arabica coffee is a typical wet fermentation. its excellent quality is closely related to microbes in the process of fermentation. the purpose of this study was to isolate and identify the microorganisms in the wet method of coffee processing in yunnan province, china. microbial community structure and dominant bacterial species were evaluated by traditional cultivated separation method and pcr-dgge technology, and were further analyzed in combination with the ...201627113591
potential antimicrobial and antiproliferative activities of autochthonous starter cultures and protease epg222 in dry-fermented sausages.this work studied the presence of nitrogen compounds with bioactive properties in iberian pork sausages that were manufactured using different autochthonous starter cultures (pediococcus acidilactici ms200 and staphylococcus vitulus rs34) and protease epg222. nitrogen compounds were extracted and evaluated for their antimicrobial effect against spoilage and pathogenic bacteria, such as bacillus cereus, escherichia coli, salmonella choleraesuis, staphylococcus aureus and listeria monocytogenes, a ...201627112426
optimization of pulsed-field gel electrophoresis procedure for bacillus cereus.in order to develop a rapid and reliable method for b. cereus genotyping, factors influencing pfge results, including preparation of bacterial cells embedded in agarose, lysis of embedded cells, enzymatic digestion of intact genomic dna, and electrophoresis parameters allowing for reproducible and meaningful dna fragment separation, were controlled. optimal cellular growth (luria-bertani agar plates for 12-18 h) and lysis conditions (4 h incubation with 500 µg/ml lysozyme) produced sharp bands on ...201627109136
alternative biotransformation of retinal to retinoic acid or retinol by an aldehyde dehydrogenase from bacillus cereus.a novel bacterial aldehyde dehydrogenase (aldh) that converts retinal to retinoic acid was first identified in bacillus cereus the amino acid sequence of aldh from b. cereus (bcaldh) was more closely related to mammalian aldhs than to bacterial aldhs. this enzyme converted not only small aldehydes to carboxylic acids but also the large aldehyde all-trans-retinal to all-trans-retinoic acid with nad(p)(+) we newly found that bcaldh and human aldh (aldh1a1) could reduce all-trans-retinal to all-tra ...201627107124
unicellular cyanobacteria synechocystis accommodate heterotrophic bacteria with varied enzymatic and metal resistance properties.the interactions between heterotrophic bacteria and primary producers have a profound impact on the functioning of marine ecosystem. we characterized the enzymatic and metal resistance properties of fourteen heterotrophic bacteria isolated from a unicellular cyanobacterium synechocystis sp. that came from a heavy metal contaminated region of cochin estuary, southwest coast of india. based on 16s rrna gene sequence similarities, the heterotrophic bacteria were grouped into three phyla: namely act ...201627106264
two distinct pathways lead bacillus thuringiensis to commit to sporulation in biofilm.the spore-forming bacterium bacillus thuringiensis is an efficient biofilm producer, responsible for persistent contamination of industrial food processing systems. b. thuringiensis biofilms are highly heterogeneous bacterial structures in which three distinct cell types controlled by quorum sensing regulators were identified: plcr-controlled virulent cells, nprr-dependent necrotrophic cells and cells committed to sporulation, a differentiation process controlled by rap phosphatases and spo0a-p. ...201627106256
genome sequence of bacillus cereus phage vb_bces-my192.italic! bacillus cereusis an opportunistic foodborne pathogen. the phage vb_bces-my192 was isolated from italic! b. cereus192 in a cooked rice sample. the temperate phage belongs to the italic! siphoviridaefamily, italic! caudoviralesorder. here we announce the phage genome sequence and its annotation, which may expand the understanding of italic! b. cereussiphophages.201627103733
chemical composition, antioxidant and antibacterial activities of essential oils from ferulago angulata.ferulago angulata boiss. (apiaceae), a perennial aromatic herb, grows wild in iran. the aerial parts of f. angulata are used as a flavouring in foods, especially dairy foods by indigenous people in western and southwestern iran.201627102982
diversity of indigenous endophytic bacteria associated with the roots of chinese cabbage (brassica campestris l.) cultivars and their antagonism towards pathogens.the study aimed to reveal the diversity of endophytic bacteria in the roots of chinese cabbage (cc) cultivated in two areas in korea, namely, seosang-gun (ss) and haenam-gun (hn), and also in a transgenic plant (tp) from the laboratory. a total of 653 colonies were isolated from the interior of cc roots, comprising 118, 302, and 233 isolates from ss, hn, and tp samples, respectively. based on 16s rrna gene sequence analysis, the isolates belonged to four major phylogenetic groups: high-g+c gram- ...201627095454
cleaning and disinfection of bacillus cereus biofilm.methodology has been evolving for the testing of disinfectants against bacterial single-species biofilms, as the difficulty of biofilm remediation continues to gain much-needed attention. bacterial single-species biofilm contamination presents a real risk to good manufacturing practice-regulated industries. however, mixed-species biofilms and biofilms containing bacterial spores remain an even greater challenge for cleaning and disinfection. among spore-forming microorganisms frequently encounte ...201627091884
microbiological and modeling approach to derive performance objectives for bacillus cereus group in ready-to-eat salads.in this article, the performance objectives (pos) for bacillus cereus group (bc) in celery, cheese, and spelt added as ingredients in a ready-to-eat mixed spelt salad, packaged under modified atmosphere, were calculated using a bayesian approach. in order to derive the pos, bc detection and enumeration were performed in nine lots of naturally contaminated ingredients and final product. moreover, the impact of specific production steps on the bc contamination was quantified. finally, a sampling p ...201627088886
different antibacterial activity of novel theophylline-based ionic liquids - growth kinetic and cytotoxicity studies.the aim of this study was to investigate novel theophylline-based ionic liquids and their cytotoxic effects towards model gram-positive and gram-negative bacteria (bacillus cereus and escherichia coli, respectively). growth kinetics, respiratory rates and dehydrogenase activities were studied in the presence of ionic liquids at concentrations ranging from 10 to 1000mg/l. additionally, the influence of ionic liquids on bacterial cells associated with specific interactions based on the structure o ...201627082812
discovery and characterization of bicereucin, an unusual d-amino acid-containing mixed two-component lantibiotic.lantibiotics are a group of ribosomally synthesized and post-translationally modified peptides (ripps) exhibiting antimicrobial activity. they are characterized by the presence of the thioether-containing bisamino acids lanthionine and methyllanthionine. here, we report a two-component lantibiotic from bacillus cereus sj1 with unusual structural features that we named bicereucin. unlike all previous two-component lantibiotics, only one of the two peptides of bicereucin contains a lanthionine. th ...201627074593
rhizosphere of avicennia marina (forsk.) vierh. as a landmark for polythene degrading bacteria.due to high durability, cheap cost, and ease of manufacture, 311 million tons of plastic-based products are manufactured around the globe per annum. the slow/least rate of plastic degradation leads to generation of million tons of plastic waste per annum, which is of great environmental concern. of the total plastic waste generated, polythene shared about 64 %. various methods are available in the literature to tackle with the plastic waste, and biodegradation is considered as the most accepted, ...201627072028
synthesis and biological evaluation of novel structural hybrids of benzofuroxan derivatives and fluoroquinolones.a series of novel hybrids based on benzofuroxan derivatives and fluoroquinolones (4a-d-6a-d) have been synthesized. unexpectedly, the reactions have resulted in salt products formation during the hydrolysis of benzofuroxans by water molecules being present in the solvent instead of usual substitution products. all the compounds have been screened for antimicrobial and toxic activities. all resulting compounds retain high activity characteristic for fluoroquinolones. many of the salts based on be ...201627061980
bacillus cereus response to a proanthocyanidin trimer, a transcriptional and functional analysis.proanthocyanidins are abundant in peanut skin, and in this study, the antibacterial effects of a peanut skin extract (pse) against food-borne bacteria were investigated to find its minimum inhibitory concentration. food-borne gram-positive bacteria, and in particular bacillus cereus, was more sensitive to pse. in particular, the inhibitory activity of epicatechin-(4β → 6)-epicatechin-(2β → o→7, 4β → 8)-catechin (eec), a proanthocyanidin trimer from peanut skin, against b. cereus was stronger tha ...201627061585
occurrence of toxigenic bacillus cereus and bacillus thuringiensis in doenjang, a korean fermented soybean paste.this study determined the prevalence and toxin profile of bacillus cereus and bacillus thuringiensis in doenjang, a fermented soybean food, made using both traditional and commercial methods. the 51 doenjang samples tested were broadly contaminated with b. cereus; in contrast, only one sample was positive for b. thuringiensis. all b. cereus isolates from doenjang were positive for diarrheal toxin genes. the frequencies of nheabc and hblacd in traditional samples were 22.7 and 0%, respectively, w ...201627052865
microbial dynamics during shelf-life of industrial ricotta cheese and identification of a bacillus strain as a cause of a pink discolouration.dairy products are perishable and have to be preserved from spoilage during the food chain to achieve the desired shelf-life. ricotta is a typical italian soft dairy food produced by heat coagulation of whey proteins and is considered to be a light and healthy product. the shelf-life of ricotta could be extended, as required by the international food trade market; however, heat resistant microflora causes spoilage and poses issues regarding the safety of the product. next-generation sequencing ( ...201627052696
optimization of process parameters for the rapid biosynthesis of hematite nanoparticles.hematite (α-fe2o3) nanoparticles are widely used in various applications including gas sensors, pigments owing to its low cost, environmental friendliness, non-toxicity and high resistance to corrosion. these nanoparticles were generally synthesized by different chemical methods. in the present study, nanoparticles were synthesized rapidly without heat treatment by biosynthesis approach using culture supernatant of bacillus cereus svk1. the physiochemical parameters for rapid synthesis were opti ...201627045277
development of a powder formulation based on bacillus cereus sensu lato strain b25 spores for biological control of fusarium verticillioides in maize plants.maize is an economically important crop in northern mexico. different fungi cause ear and root rot in maize, including fusarium verticillioides (sacc.) nirenberg. crop management of this pathogen with chemical fungicides has been difficult. by contrast, the recent use of novel biocontrol strategies, such as seed bacterization with bacillus cereus sensu lato strain b25, has been effective in field trials. these approaches are not without their problems, since insufficient formulation technology, ...201627038945
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