isolation of two new related peptide antibiotics, cerexins a and b (studies on antibiotics from the genus bacillus. i).two new antibiotics cerexins a and b were isolated from different strains identified with bacillus cereus. these two antibiotics are amphoteric in nature, soluble in particular solvents such as dimethylsulfoxide, dimethylformamide and alkaline water, and show typical infrared absorptions of peptide. these also have similar antimicrobial properties active against gram-postive bacteria.1975236271
the influence of growth and sporulation temperature on heat resistance of a bacterial nucleosidase. 1975236744
inositol cyclis phosphate as a product of phosphatidylinositol breakdown by phospholipase c (bacillus cereus). 1975236918
effect of ph on sporulation of bacillus improved broth medium was developed for high growth yields of bacillus subtilis var. niger ncib 8649, bacillus cereus ncib 9373, and bacillus stearothermophilus ncib 8919 and atcc 7953. sporulation was abundant (1.1 times 10-8 b. subtilis var. niger and 9.2 times 10-7 b. cereus per ml) at an initial ph of 7.0. sporulation of both strains of b. stearothermophilus took place (1.9 times 10-7 and 2.4 times 10-7/ml, respectively) in this medium when initial ph values of 7.7 to 8.7 were used.1975238470
[formation by bacteria of the genus bacillus of coagulases that coagulate plasma]. 1975240446
biological characteristics of an enterotoxin produced by bacillus enterotoxin synthesized during exponential growth by bacillus cereus produces fluid accumulation in rabbit ileal loops, alters vascular permeability in the skin of rabbits, and kills mice when injected intravenously. all activities are eluted simultaneously from a sephadex g-75 column and are distinct from the hemolysin and egg yolk turbidity factor of b. cereus. the enterotoxin is a true exotoxin. it interacts with intestinal receptor sites in a highly transient manner in the ileal loop syst ...1975240499
[report of "lethal factor" synthetized by bacillus cereus (author's transl)].bacillus cereus growing in nutrition broth produces during logarithmic growth and in stationary phase a lethal factor extremely toxic for rodents. this toxin is only produced in the presence of oxygen and depends on bacterial replication. the highest titers of toxin are obtained using bacteria in a concentration of 10(7) to 10(9) per ml of nutrition broth. during regression phase of bacillus cereus the titer of toxin declines to zero. toxicity of the lethal factor becomes not altered in an alcal ...1975242167
production and property of beta-lactamases in streptomyces.the production of beta-lactamases by 100 strains of streptomyces was studied. about one-half of the strains produced more than 2.3 u of beta-lactamase per ml, and another half produced less than 1.4 u/ml. the amounts of beta-lactamases produced by two strains were in the order of those produced by bacillus cereus 569/h and bacillus licheniformis 749/c. these streptomyces enzymes function primarily as penicillinases rather than cephalosporinases. properties such as ph optimum, substrate specifici ...1975242252
cardiolipin: a stereospecifically spin-labeled analogue and its specific enzymic hydrolysis.the spin-labeled cardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-(16-doxylstearoyl)glycero(3)phosphol]-sn-glycerol has been prepared. the stereoselective synthesis makes use of the monolysocardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-lyso-sn-glycero(3)phospho]-sn-glycerol, available from the stereospecific hydrolysis of cardiolipin by phospholipase a2 (phosphatide 2-acylhydrolase, ec of trimeresurus flavoviridis. the results of treatment of the spin-labeled cardiolipin with the cardiolip ...1978274715
predicted secondary structures of four penicillin beta-lactamases and a comparison with two lysozymes.we have predicted the secondary structures of four beta-lactamases (bacillus cereus, bacillus licheniformis, staphylococcus aureus, and escherichia coli r-tem) by the statistical method of chou & fasman as well as by the information theory method of garnier et al. the secondary structures of all four beta-lactamases are of the alpha/beta type (levitt & chothia's nomenclature), with helices at n- and c-termini. there are about eight short regions each of alpha-helical (30--50%) and beta-strand (1 ...1979313376
identification of histidine residues that act as zinc ligands in beta-lactamase ii by differential tritium exchange.1. four histidine-containing peptides have been isolated from a tryptic digest of the zn2+-requiring beta-lactamase ii from bacillus cereus. one of these peptides probably contains two histidine residues. 2. the presence of one equivalent of zn2+ substantially decreases the rate of exchange of the c-2 proton in at least two and probably three of the histidine residues of these peptides for solvent 3h. 3. it is concluded that peptides containing at least two of the three histidine residues acting ...1979314287
membrane ultrastructural changes during calcium phosphate-induced fusion of human erythrocyte ghosts.nascent calcium phosphate promotes the agglutination and fusion of human erythrocyte ghosts. membrane phospholipids of erythrocyte ghosts treated with ca2+ and phosphate ions become exposed to attack by phospholipase c (phosphatidylcholine cholinephosphohydrolase, ec (bacillus cereus). freeze-fracture pictures of fused erythrocyte ghosts show the presence of regions deficient in intramemebrane particles in the protoplasmic face which we believe to be regions of fusion. discontinuous reg ...1977329283
septicemia in children with cancer.because of the persistently high mortality from sepsis in cancer patients, a retrospective study was designed to identify the causative organisms and to determine the factors affecting the outcome of sepsis. a total of 84 episodes of septicemia in 61 children with cancer were studied. the more frequently isolated organisms were: staphylococcus aureus (21.4%); escherichia coli (18%); klebsiella (7.1%); pseudomonas (6%); and bacteroides fragilis (6%). other isolates included proteus, serratia, aci ...1979371000
the variable response of bacteria to excess ferric iron in host tissues.the enhancement by exogenous ferric iron, both systemic and local, of the infectivity of 120 strains of bacteria, representing 17 genera, was measured in the skin of guinea-pigs. systemic iron enhanced only 23% of 115 strains, and local iron 49% of 71 strains. systemic iron, by an apparently anti-inflammatory action, depressed the size of lesions produced by 27 of the non-enhanced strains from nine of the genera tested. for most strains, the degree of enhancement was small, ranging from 2- to 8- ...1979372534
serotypes of bacillus cereus from outbreaks of food poisoning and from routine foods.a provisional serotyping scheme was used to type cultures of bacillus cereus from 84 outbreaks of food poisoning in seven countries; 283 of the 337 (84%) cultures tested were typable. in 35 of the 61 outbreaks associated with a vomiting-type syndrome, foods, clinical specimens or both yielded h-serotype 1 only. type 1 strains together with other sterotypes were isolated in seven outbreaks. in 14 outbreaks types 3, 4, 5, 8 or a mixture of serotypes were present. untypable strains were isolated in ...1977401846
bacillus cereus pneumonia. survival in a patient with cavitary disease treated with gentamicin.cavitary pneumonitis due to bacillus cereus occurred in a patient with acute lymphoblastic leukemia. pneumonia due to this organism is uncommon and recovery has not been previously reported. the present report is the first description of cavitary pneumonia due to b. cereus with a successful outcome. recovery was attributed to appropriate antimicrobial therapy and induction of leukemic remission with chemotherapy.1977402096
biochemical properties of clostridium bifermentans previously found for spores of bacillus species, dormant spores of clostridium bifermentans contained essentially no adenosine triphosphate, a high level of adenosine monophosphate, a high level of 3-phosphoglyceric acid, and much transfer ribonucleic acid lacking a 3'-terminal adenosine monophosphate residue. as in spores of bacillus species, germination of c. bifermentans spores was accompanied by utilization of the 3-phosphoglyceric acid, a large increase in the adenosine triphosphate leve ...1977402349
rapid fluorimetric assay of minocycline in plasma or serum: comparison with microbiological assay.1. plasma concentrations of minocycline were assayed by measuring the fluorescence emission of an aluminum-minocycline chelate in a spectrofluorimeter. 2. the assay gives reproducible results over the concentration range 0.05-5.0 mug ml-1, and compared favourably with results achieved using a conventional large plate agar diffusion microbiological assay in a double-blind comparison of the two methods. 3. fluorimetric assay of minocycline in plasma was found to correlate closely with antibiotic a ...1977402926
initiation of bacillus spore germination by hydrostatic pressure: effect of temperature.suspensions of bacillus cereus t, b. subtilis, and b. pumilus spores in water or potassium phosphate buffer were germinated by hydrostatic pressures of between 325 and 975 atm. kinetics of germination at temperatures within the range of 25 to 44 degrees c were determined, and thermodynamic parameters were calculated. the optimum temperature for germination was dependent on pressure, species, suspending medium, and storage time after heat activation. germination rates increased significantly with ...1977403174
phospholipase c from bacillus cereus. evidence for essential lysine residues.1. phospholipase c was inactivated by exposure to the three amino-group reagents, ethyl acetamidate, 2,4,6-trinitrobenzensulphonic acid and pyridoxal 5'-phosphate plus reduction. 2. inactivation by pyridoxal 5'-phosphate showed the characteristics of schiff's base formation with the enzyme. the pyridoxal 5'-phosphate-treated enzyme after reduction had an absorbance maximum at 325 mm and 6-n-pyridoxyl-lysine was the only fluorescent component after acid hydrolysis. 3. for complete inactivation, 2 ...1977403907
enzymic assays for isomers of 2,6-diaminopimelic acid in walls of bacillus cereus and bacillus enzymic assay for individual isomers (meso-, ll- and dd-) of 2,6-diaminopimelate was developed. the enzyme 2,6-diaminopimelate decarboxylase specifically attacked meso-diaminopimelate and was used to measure this isomer manometrically. the meso- and ll-isomers were measured together manometrically in a coupled assay with diaminopimelate decarboxylase and diaminopimelate epimerase (which converts ll-diaminopimelate into meso-diaminopimelate). the dd-isomer was not attacked by either enzyme and ...1977403912
role of glutamate in the sporogenesis of bacillus cereus.bacillus cereus t, sporulating in a chemically defined medium under optimum conditions, requires substrate quantities of glutamate during the first 4 h of sporogenesis. seventy percent of the glutamate utilized was catabolized to co2 during this period, with the remaining glutamate carbon assimilated into various spore constituents, principally protein and nucleic acid. the importance of glutamate as the primary source of reducing potential and energy for early stages of spore formation was inve ...1977404283
precipitating antibody against protease of corynebacterium pyogenes in pigs.antibody in sera from pigs carrying an abscess associated with corynebacterium pyogenes and healthy pigs was examined by the agar gel diffusion test. in the test, the concentrated culture fluid containing the protease of c. pyogenes was used as antigen. as a result, precipitating antibody was demonstrated in sera from 25 of 30 abscessed pigs and a few of the healthy pigs. when the relationship between precipitating antibody and protease was examined by the immunoelectrophoresis and gel filtratio ...1977404574
inactivation of phospholipase c from bacillus cereus by a carboxyl group modifying reagent.phospholipase c from bacillus cereus was inactivated by incubation with either of the carboxyl reagents, a water-soluble carbodimide plus a nucleophile or woodward's reagent k. with the former reagent, the incorporation into the enzyme of the first mol of nucleophile caused a 4-5-fold increase in the km for dihexanoyllecithin with no significant effect on the vm. the second mol of nucleophile incorporated caused no further change in km but destroyed most of the catalytic activity. modification o ...1977404815
the inactivation of beta-lactamase i from b. cereus by dicloxacillin: effect of buffer composition on the kinetic characteristics of the modified enzyme.buffer composition affects the pattern of inactivation of b. cereus beta-lactamase i by dicloxacillin. at ph 7.0, in the presence of phosphate ions, dicloxacillin appears to cause essentially an irreversible inactivation of the catalytic site of the enzyme, while in a tris-cacodylate buffer the inactivation process appears to affect essentially the substrate-binding site. buffer ions act presumably by interacting themselves with either the catalytic or the substrate-binding site of the enzyme, t ...1977405348
microbial production of vitamin b12 antimetabolites. iii compound 102804 from bacillus cereus.a basic compound with empirical formula c12h16n2o5 was isolated from bacillus cereus 102804 fermentations of a soybean meal-glucose medium. the inhibitory activity of compound 102804 on growth of gram-positive and gram-negative bacteria growing in a chemically defined medium was reversed by vitamin b12, by l-methionine, and by d-methionine. it has no inhibitory activity for escherichia coli (davis 113-3) when grown in media containing l-methionine. the biosynthesis of 102804 coincides with the s ...1977405359
an investigation of the bacteriological quality of retail vanilla hundred and thiry-three vanilla slices, purchased from shops in the west yorkshire metropolitan county, were examined to determine the numbers and types of bacteria present at the time of purchase. the surface colony count at 37 degrees c was greater than 10(3)/g in 67/133 (50%) of the samples examined, bacillus cereus being found at that concentration in 21.8%, coliform bacilli including e. coli in 5.3%, staphylococcus aureus in 3-0% and streptococcus faecalis in 0-8%. thirty-four strains o ...1977405420
fate of foreign dna taken up by tonsillar lymphocytes.the uptake of heterologous (b. cereus) dna by tonsillar lymphocytes was followed for 22 hours. acid-precipitable dna became associated with the cells as soon as they were mixed with dna. the uptake was linear from 0 to 3 hours, between 3 to 6 hours part of the cell-associated dna was released into the incubation medium, and at 22 hours the amount of label in the cells reached the values measured at 2--3 hours. in respect of the size of dna the uptake seemed to follow the same saturation curve. t ...1977405837
a severe necrotic enterotoxin produced by certain food, food poisoning and other clinical isolates of bacillus cereus.the ability of certain strains of bacillus cereus consistently to elaborate a filterable non-dialysable toxin capable of causing severe disruption and necrosis of the intestinal mucosa and submucosa is confirmed. this property is not universal to all b. cereus strains and different degrees of production of this toxin are exhibited by the different strains which produce it. the necrotic effect is produced by whole-cell cultures of the toxin producing strains in broth and in boiled rice. some char ...1977405982
severe systemic and pyogenic infections with bacillus cereus. 1977406000
the effect of storage on drugs incorporated into pelleted fish food. 1977406257
a phosphatidylinositol phospholipase c (phosphatasemia factor)of bacillus cereus. 1977406439
study of inhibition of outgrowth in bacillus cereus t by ethyl picolinate.the effects of ethyl picolinate on germination, outgrowth, and sporulation of bacillus cereus t were studied in a synthetic medium containing glucose. ethyl picolinate specifically inhibited at two stages, outgrowth and sporulation. the initiation of germination and cell division was not affected. the inhibition of outgrowth by ethyl picolinate could be reversed by enrichment of inoculum with aspartic acid, asparagine, lysine, phenylalanine, and tyrosine among the amino acids and by oxalacetate. ...1977406840
microbiological transformations of delta6a10a-tetrahydrocannabinol.a screening program was conducted to find microorganisms that catalyze transformation reactions with cannabinoids. three hundred fifty-eight cultures, consisting of 97 bacteria, 175 actinomycetes, and 86 molds, were incubated in media containing 0.5 mg of delta(6a,10a)-tetrahydrocannabinol (delta(6a,10a)-thc) per ml. after 120 h of cultivation, ethyl acetate extracts of the cultures were examined by thin-layer chromatography (tlc) for transformation products. about 18% of the cultures modified d ...1977406841
studies on the disaggregation of ef-1 with carboxypeptidase a. 1977406850
isolation and characterization of structural components of bacillus cereus ahu 1356 cell walls. 1977407078
the action of lysozyme on peptidoglycan with n-unsubstituted glucosamine residues. isolation of glycan fragments and their susceptibility to lysozyme.1. a peptidoglycan preparation n-acetylated at about 30% of glucosamine residues was obtained by the treatment of the lysozyme-resistant cell wall paptidoglycan of bacillus cereus with acetic anhydride at ph 7. fractionation of dialyzable material resulting from lysozyme digestion of the glycan component of this peptidoglycan preparation yielded five oligosaccharides designated as s1 to s5 besides the disaccharide glcnac-murac. 2. oligosaccharide s3, which accounted for about 30% of the disaccha ...1977407083
spores of microorganisms. xxvii. some physiological and morphological characteristics of thick-walled cellular forms of bacillus cereus. 1977407136
microbiol n-acetylation of daunorubicin and daunorubicinol. 1977407204
production of copper coproporphyrin iii by bacillus cereus. i. purification and identification of copper coproporphyrin iii.bacillus cereus strains 2 and t did not form spores and accumulated a large amount of purple pigment inside the cells, when cultured in a yeast extract-ammonium salt medium with excess glucose. the pigment was extracted and crystallized as the ethyl ester. it was identified as copper coproporphyrin iii.1977407221
production of copper coproporphyrin iii by bacillus cereus. ii. regulation of the biosynthesis of coproporphyrin iii and its copper complex by oxygen and heavy metal ions.the effects of oxygen and heavy metal ions on the production of copper coproporphyrin iii were studied in bacillus cereus strain 2. the formation of copper coproporphyrin iii was found to be maximum when the cells were cultivated in g-medium at a low level of oxygen supply, but it was suppressed at extremely low oxygen supply levels. when the cells were cultured in metal-free g-medium, neither metal-coproporphyrin iii nor coproporphyrin iii was formed. in the presence of copper in the medium (40 ...1977407222
a case of bacillus cereus bacteraemia.a case is presented of bacillus cereus bacteraemia in a patient receiving hepatic perfusion with 5-fluorouracil (5fu) for metastasis from a carcinoma of the breast. the literature concerning systemic b. cereus infections is briefly reviewed.1977407561
[detection of bacillus cereus-toxins (author's transl)].during the logarithmic state of growth, b. cereus produced extracellular toxins, which could be precipitated by ammoniumsulphate. the toxins were not dialysable. 9 strains of b. cereus were tested and showed lethal, hemolytic, and phospholipase-c-reactions. the lethal toxin injected i.v., killed mice within 20 minutes. an intradermal injection of the 9 toxin-precipitates in rabbits produced skin reactions, which could be neutralized by a specific antiserum against the b. cereus strain b-4ac. rel ...1977407743
inhibition of the synthesis of phospholipase c in bacillus cereus by a component of the growth medium.a low molecular weight compound, probably of peptide nature, present in the beef heart extract component of the growth medium, inhibits post-transcriptionally the biosynthesis of phospholipase c by a strain of bacillus cereus. the compound also prevents the increase of proteolytic activity in the growth medium, thus suggesting that the synthesis of another enzyme, an extracellular protease, is also inhibited, and that the inhibitory compound may therefore have a more general effect on exoenzyme ...1977407774
epoxidation of aldrin to exo-dieldrin by soil bacteria.twenty-two strains of soil bacteria, including representatives of the genera bacillus, micromonospora, mycobacterium, nocardia, streptomyces, thermoactinomyces, and pseudomonas and 10 unidentified gram-negative, motile, rod-shaped bacteria, were shown to degrade aldrin to its epoxide dieldrin. in every case, the exo-stereoisomer of dieldrin was produced exclusively.1977407844
studies on bacterial cell wall inhibitors. ii. inhibition of peptidoglycan synthesis in vivo and in vitro by amphomycin. 1977407940
[regulation of isoleucine and valine biosynthesis in bacillus cereus t: possible role of threonine deaminase].threonine desaminase: a possible regulatory element suggested by the analysis of isoleucine, valine pathway regulation in bacillus cereus t.1977408042
re-activation of guinea-pig liver uridine diphosphate glucuronosyltransferase with 1-monoacyl phosphatidylcholine after extensive phospholipid deletion at 5 degrees c with pure bacillus cereus phospholipase c. 1977408208
[heterogeneity of the microcyclic development of bacillus cereus 504]. 1977408588
studies on the in vivo cellular reactions of insects: an ultrastructural analysis of nodule formation in galleria mellonella.cellular aggregates or nodules which formed in galleria mellonella larvae in response to injections of killed bacillus cereus were examined during their first 24 hr of development. the initial aggregation stage was very rapid, occurring within 1 min, and was brought about by the release of an adhesive flocculent material by granular haemocytes in contact with the bacteria. this material, which subsequently became increasingly electron dense owing to melanin deposition, consolidated and by 5 min ...1977408940
antimicrobial activity of amine oxides: mode of action and structure-activity correlation.the effect of n-alkyl derivatives of saturated heterocyclic amine oxides on the growth and metabolism of microorganisms has been studied. 4-dodecylmorpholine-n-oxide inhibited the differentiation and growth of bacillus cereus, of different species of filamentous fungi, and of the yeast saccharomyces cerevisiae. for vegetative cells, the effect of 4-dodecylmorpholine-n-oxide was lethal. cells of s. cerevisiae, after interaction with 4-dodecylmorpholine-n-oxide, released intracellular k(+) and wer ...1977409340
extent of cross-linking of germ cell wall of a variant of bacillus cereus. 1977409409
immunocytochemical localization of spore specific antigens in ultrathin sections. 1977409707
[a fatal food poisoning caused by bacillus cereus (author's transl)].the autopsy of a 37 year old man who had died under extraordinary circumstances showed a bromatoxism by bacillus cereus. the verification of the bacillus was possible although the man had already died 2-3 days before; and that in spite of the existence of early beginning rottenness. with the help of morphological findings the pathogenetic effect of the toxins (exotoxin and enterotoxin) of bacillus cereus is discussed. blood cultures of the heart alone are not sufficient to prove a causal connexi ...1977410193
comparative studies of bacillus cereus strains isolated from various foods and food poisoning outbreaks. 1977410239
effect of some bacterial products on platelet electrophoretic mobility (pem).bacterial lps, used in our experiments, are able to decrease pem at concentration of 100 microgram/ml after incubation with 1 x 10(7) platelets/ml for 30 min at 37 degrees c. such effect is statistically significant for bacteroides fragilis lps, s. enteritidis and s. typhi. e. coli lps 026:b6 w and e. coli 055:b5 w are not effective at all, s. minnesota (incomplete) lps 595 and 345 free of 0-polysaccharide and partially lacking core in their molecule did not influence pem, while s. minnesota lps ...1977410424
characterization of the "microprotease" from bacillus cereus. a zinc neutral endoprotease.the neutral protease isolated from bacillus cereus (brl-70) has been purified by affinity chromatography and characterized. the enzyme exhibits a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, has a molecular weight of 34 000 by ultracentrifugation, and contains one enzymatically essential zinc atom per 34 000 g. these data together with the amino acid composition, response to metal substitution, chemical modification, and substrate specificity all indicate that this p ...1977410441
levels of acetyl coenzyme a, reduced and oxidized coenzyme a, and coenzyme a in disulfide linkage to protein in dormant and germinated spores and growing and sporulating cells of bacillus megaterium.dormant spores of bacillus megaterium were found to contain approximately 850 pmol of coenzyme a (coa) per milligram of dry weight. of this total, less than 1.5% was acetyl-coa, 25% was coa-disulfide, 43% was in disulfide linkage to protein, and the remainder was the free thiol. dormand spores of bacillus cereus and clostridium bifermentans contained 700 and 600 pmol of coa per milligram of dry weight, respectively; in both species approximately 45% of the coa 45% of the coa was in disulfide lin ...1977410791
[the occurrence of bacillus cereus in dairy products (authors transl)].during one year 1390 samples of pasteurized milk products from five different dairies were examined for content of b. cereus. the examination was done on the day of packaging and after incubation at 17 degrees c for 24 hrs. as well. on examination on the day of packaging 8.1 % were positive. no difference could be demonstrated between low-temperature pasteurized and high-temperature pasteurized products. however, differences were found from one plant to another (2.1 %--14.5 %). after incubation ...1977411110
the fate of enterotoxigenic bacteria inoculated onto chinese barbecued foods. 1977411566
[detection of cephalosporins in raw milk with the aid of b. cereus beta lactamase]. 1977411648
relationship between the structure of carbonylcyanide phenylhydrazones and inhibition of growth of microorganisms, stimulation of respiration of yeast cells and rat liver mitochondria.the effect of ten derivatives of carbonylcyanide phenylhydrazone on growth of bacteria, yeast and different species of filamentous fungi was investigated. in yeast and mitochondria isolated from rat liver the effect of these derivatives on the respiratory activity was also followed. the relative efficiency of the individual derivatives of carbonylcyanide phenylhydrazone was determined on the basis of the results obtained. it was shown that derivatives, in which the substituent on the benzene rin ...1977411721
a survey for the presence of teichuronic acid in walls of bacillus megaterium and bacillus cereus. 1977411891
[phage diagnosis of strains of bacillus cereus isolated from food products].a total of 80 cultures of bacillus cereus, isolated from food products, were studies to establish the behaviour to specific phages or the cam set and the presence of lysogeny. a modern bacteriophage method was tested for the first time in the differentiatons of bacillus cereus, which was so far studied mainly in the differentiation of bacillus anthracis. results showed that bacteriophagy with specific type phages as a method, though not absolutely precise, is dependable for the differentiation o ...1977412296
characterization and function of intracellular proteases in sporulating bacillus cereus.intracellular proteases from sporulating bacillus cereus have been purified by ammonium sulfate fractionation, heat treatment and deae cellulose column chromatography. after the last purification step, two protease activities, with an activity ratio of about thirty to one are resolved. both proteases are resistant to o-phenanthroline but sensitive to phenyl methyl sulfonyl fluoride. their separation by polyacrylamide gel electrophoresis and deae cellulose column chromatography, their difference ...1977412478
[the effect of sodium fluoride on the antimicrobial action of tetracycline]. 1977412595
[hygienic evaluation of insecticide preparations]. 1977412732
the effect of water activity and aw-controlling solute on sporulation of bacillus cereus t. 1977412827
characterization of a bacillus cereus protease mutant defective in an early stage of spore germination.temperature-sensitive sporulation mutants of bacillus cereus were screened for intracellular protease activity that was more heat labile than that of the parental strain. one mutant grew as well as the wild type at 30 and 37 degrees c but sporulated poorly at 37 degrees c in an enriched or minimal medium. these spores germinated very slowly in response to alanine plus adenosine or calcium dipicolinate. during germination, spores produced by the mutant rapidly became heat sensitive, but released ...1978412836
the role of various milk fractions and the importance of somatic cells in the formation of germinant(s) for bacillus cereus when milk is pasteurized. 1977412883
bacillus cereus food poisoning. 1978413036
lipoquinones of some bacteria and mycoplasmas, with considerations on their functional a comparative study the lipoquinones of some chemoorganotrophic, facultatively aerobic bacteria, and representative acholeplasma, mycoplasma, spiroplasma, and thermoplasma strains were investigated. the quinones were partly purified by preparative thin layer chromatography of lipid extracts, and characterized by their difference spectra (reduced minus oxidized) and rf values. respiring bacteria expectedly contained benzoquinones and/or naphthoquinones in micromolar concentrations whereas some ...1977413478
the histidine residues of phospholipase c from bacillus cereus.the inactivation of phospholipase c from bacillus cereus at ph6 by diethyl pyrocarbonate parallelled the n-ethoxyformylation of a single histidine residue in the enzyme. the inactivation arose from a decrease in the maximum velocity of the enzymic reaction with no effect on the km value. the inactivation did not apparently alter the ability of the enzyme to bind to a substrate-based affinity gel. the native enzyme contained only one reactive histidine residue. removal of the two zinc atoms from ...1977413541
[admixtures of antibiotic substances in enzyme preparations of microbial origin intended for use as food additives].a group of enzymtic preparations, mainly of the soviet make were investigated for the presence of antibiotic substances admixtures. altogether 25 preparations obtained from molds, actinomycetes, bacteria and yeast were studied. the results showed that 18 preparations contained antibiotic substances in an amount of 0.6-1300 u, equivalent to oxytetracycline units per 1 g. most often antibiotics were present in preparations of the pectinase and protease group. in the group of glucoamylase the antib ...1977414448
[clinico-epidemiologic characteristics of group illness caused by bac. cereus food poisoning].the authors describe an outbreak of food poisoning caused by bac. cereus embracing 80% of persons who catered at the same catering establishment. the poisoning occurred in the course of 15 hours after the moment of eating a sauce stored in warm place. in the majority of cases the course of the disease was mild; 77.8% of the patients displayed phenomena of enteritis, 20.2%--of entercolitis, and 2.0%--of gastroenterocolitis. all the patients recovered without any treatment in the course of 1--2 da ...1977414486
alteration by cereolysin of the structure of cholesterol-containing membranes.when erythrocyte membranes were treated with cereolysin, negatively stained and examined by electron microscopy, ring and arc-shaped structures were observed in the membrane. the outside diameter of the rings varied from 33 to 50 nm with a border thickness of 6.7 to 8.3 nm. the arcs varied in length from 33 to 170 nm with a border thickness of also 6.7 to 8.3 min. when right-side-out erythrocyte ghosts which had been treated with cereolysin were examined by electron microscopy after freeze-fract ...1978414780
spore location patterns in sporulating doublets of bacillus cereus and bacillus megaterium, derived from single doublet isolates with differing sporulation geometry.the distribution of spore loci in pairs of bacillus cereus and b. megaterium showed different degrees of polarization from random location towards location at the old end of the sporangium. when individual doublets containing both spores at either the old or new ends of the sporangia were isolated by micromanipulation, subsequent culture showed the same spore location patterns.1978415036
membrane-bound nucleotidase of bacillus cereus.a membrane-bound nucleotidase of bacillus cereus t was solubilized by digestion with trypsin and subsequently purified more than 300-fold. the purified nucleotidase was most active on ribonucleoside 5'-monophosphates and was slightly less active (40 to 60%) on deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates. in addition to hydrolytic activity, the nucleotidase preparation possessed phosphotransferase activity by which phosphate is transferred from a phosphate donor to ...1978415044
bacteriocin and antibiotic resistance plasmids in bacillus cereus and bacillus subtilis.a number of plasmids have been isolated as covalently closed circular dnas from strains of bacillus cereus and b. subtilis. from 12 out of 15 strains of b. cereus, plasmids could be isolated. most of the b. cereus strains contained two or more plasmids. their molecular weights ranged from 1.6 x 10(6) to 105 x 10(6). bacteriocin production could be attributed to a 45 x 10(6)-dalton plasmid (pbc7) from b. cereus dsm 336, and tetracycline resistance to a 2.8 x 10(6) plasmid (pbc16) from b. cereus g ...1978415051
[germination and outgrowth of spores and proliferation of outgrown vegetative cells in the peritoneal cavity of mice intraperitoneally injected with bacillus cereus or bacillus subtilis spores (author's transl)]. 1977415160
reversible inhibitors of penicillinases.reversible competitive inhibitors of a penicillinase, beta-lactamase 1 from bacillus cereus, were studied. these represent the first inhibitors of a penicillinase that lack the beta-lactam ring. the products of the enzymic reaction, namely penicilloic acids, are inhibitors; their decarboxylation products, the penilloic acids, are also inhibitors, and have somewhat lower ki values. inhibitors have been prepared from benzylpenicillin, phenoxymethyl-penicillin, methicillin (2,6-dimethoxybenzamidope ...1978415738
induction of penicillinase by bacitracin. 1978415741
prevalence of bacillus cereus in the faeces of healthy a survey designed to determine the prevalence of bacillus cereus in the faeces of healthy persons, the organism was found in low numbers in 100 (14%) of single faecal specimens from 711 adults in the general population. in addition, in an attempt at assessing the changes in the b. cereus distribution within the faecal flora of the individual, weekly faecal specimens were submitted over a seven-week period by 18 members of staff of two laboratories. the total isolation rate was again 14%, with ...1978416141
[a case of acute myelogenous leukemia, complicated with bacillus cereus septicemia (author's transl)]. 1977416149
[effect of a bacillus cereus 504 culture on the development of homologous strain cells]. 1978416328
[methods of demonstrating antibiotics residues in meat, organs and eggs]. 1977416557
[effect of substituted isatinisothiosemicarbazones on the multiplication of bacteria]. 1977416604
[on the utilization different organic and anorganic compounds by some strains of bac. cereus var. mycoides (flügge) (author's transl)].in order to investigate the decomposition of 46 organic and anorganic compounds by bac. mycoides, the behaviour of four strains was tested by means of plate counting technique (after koch) and the warburg method. mostly intensive substances containing protein were decomposed by all strains. furthermore amino acid, sugar, alcohols, and ammonium salts are used by the bacteria, but there were large differences between strains tested. urea and nitrates have not been decomposed.1977416618
determination of fatty acid compositions of bacillus cereus and related bacteria: a rapid gas chromatographic method using a glass capillary column.a rapid gas chromatographic method for the determination of fatty acid compositions of bacillus cereus and related bacteria is presented. by the use of a free fatty acid phase-coated glass capillary column, the complete separation of fatty acids, including the branched ones, was achieved. the method enables a more distinct differentiation of bacillus species than can be obtained with packed columns.1978416751
fatty acids of gram-positive bacterial rods from human dental plaque. 1978416814
[radiosensitivity of the bacteriophages of aerobic sporulating microorganisms]. 1978417102
low temperature microspectrofluorometry: design of a 'cold chamber'.low temperature microspectrofluorometry allows an improvement of spectral resolution and an increase of fluorescence intensity. suppression of fluorescence fading, or at least a marked reduction, is also obtained. a cooling chamber for microspectrofluorometric measurement is described which allows the cooling, under a microscope, of a biological sample down to liquid nitrogen temperature. objectives with numerical apertures better than 1.0 and a magnification power up to 100x can be used. low te ...1978417183
osmotically induced increase in thermal resistance of heat-sensitive, dipicolinic acid-less spores of bacillus cereus ht-8.thermal resistance in heat-sensitive, dipicolinic acid (dpa)-less spores of bacillus cereus ht-8 heated in sucrose solutions increased at and above a concentration of 2 m sucrose. the decimal reduction times at 75 degrees c for spores heated in 0.0, 1.8, 2.2, and 2.6 m sucrose were 2.0, 2.8, 4.5, and 12 min, respectively. maltose, fructose, and glucose increased heat resistance above that observed in water but did not elevate resistance to the level observed with sucrose at the same osmolality. ...1978417675
enzymatic formation of uridine diphosphate enzyme that catalyzes the interconversion of udp-n-acetyl-d-glucosamine and udp-n-acetyl-d-mannosamine was purified about 700-fold from the supernatant fraction of bacillus cereus, and the properties of this enzyme were studied. this enzyme was not stimulated by nad+, nadh, or any metal ions. the optimum ph was between 7.5 and 8.0. at equilibrium of the reaction, the ratio of udp-n-acetylglucosamine to udp-n-acetylmannosmaine was about 9:1. the enzyme was inactive toward free n-acetylhexosami ...1978418068
carboxymethylcellulase produced by facultative bacteria from the hind-gut of the termite reticulitermes hesperus.bacillus cereus rw1 and serratia marcescens rw3, isolated from the hind-gut of the termite reticulitermes hesperus, both grew well on mesquite wood and produced moderate amounts of carboxymethylcellulase. carboxymethylcellulose (cmc) gels were depolymerized rapidly by b. cereus rw1 and slowly by s. marcescens rw3. the depolymerization of cmc was ph and temperature sensitive. depolymerization of gels by growing cultures of b. cereus rw1 and the action of cell-free extracts of b. cereus rw1 on cmc ...1978418144
preliminary crystallographic data for beta-lactamase i from bacillus cereus 569. 1978418181
[comparative investigations on the efficiency of chlorine and ozone against bacteria and spores (author's transl)].this paper is concerned with the measurement of first order rate constants for the death of bacteria by chlorine and ozone. while the first order conditions for chlorine were achieved with the aid of a new instrument which works basing on the polarographic principle this was attained for ozone under steady state conditions. besides differences of species some different properties for both oxidants were found as far as their bactericidal efficiency is concerned. they are discussed in this paper.1978418606
infant foods as a potential source of diarrhoeal illness in rural west is common practice in rural areas of the gambia to prepare infant foods in quantities which are sufficient to meet the needs of the day rather than one meal. these are then stored at ambient temperatures for periods up to 12 hours for feeding to the child on demand. the total viable counts and levels of bacillus cereus, clostridium welchii, staphylococcus aureus and escherichia coli were determined in 294 infant foods samples from nought to eight hours after preparation. the presence of salmo ...1979442188
protection of pregnant mice with phospholipase c and with arvin against foetal death induced by bacterial lipopolysaccharide.injection of bacterial lipopolysaccharide into pregnant mice resulted in fibrinogen accumulation, thrombosis and haemorrhage in the placental tissue and foetal death. depletion of circulating fibrinogen by a thrombin-like enzyme from the venom of malayan pit viper, arvin, prevents foetal death. foetal protection was also obtained by treating the mothers with a preparation of phospholipase c from bacillus cereus known to inactivate tissue thromboplastin. it is suggested the lipopolysaccharide cau ...1979444421
use of enzymatic and electron microscopy (freeze-etching) methods for studying atp-dependent masking of erythrocyte membrane phospholipids.membrane phospholipids of atp-depleted chicken, rat, and toad erythrocytes are more susceptible than fresh cells to hydrolysis by phospholipase c (bacillus cereus), phospholipase a (bee venom), or the combination of these enzymes and sphingomyelinase. atp depletion of chicken and rat erythrocytes greatly increased the membrane phospholipid fraction, which can be extracted by dry ether. trinitrobenzene sulfonic acid attacked about 20% of the phosphatidylethanolamine of fresh chicken erythrocytes ...1979478827
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