Publications

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enzymic reduction of the c-terminal dehydroalanyl-lysine sequence in nisin. 19704992696
[lecithinase activity of bacillus cereus strains isolated from various natural sources]. 19704992749
[the value of the lecitho-vitelline reaction in the differential diagnosis of bac. cereus]. 19704992768
kinetics of n-ethylmaleimide inhibition of l-alanine-induced germination of bacillus cereus spores. 19704984014
phosphorylation of 1-alkenyl-2-acylglycerol and preparation of 2-acylphosphoglycerides. 19704983883
outbreak of food poisoning caused by bacillus cereus. 19704983427
properties of penicillinase from bacillus cereus 569. 19704986078
[effect of oleandomycin in triacetyloleandomycin on the result of determination of biological activity of the latter by agar diffusion method]. 19704986115
synthesis of a factor stimulating transcription in outgrowing bacillus cereus spores. 19704986265
in vitro determination of bacillus thuringiensis, bacillus cereus, and related bacilli. 19704986479
chemical composition of exosporium from spores of bacillus cereus.homogeneous fragments of exosporium were extricated in centigram amounts from dormant spores of bacillus cereus and analyzed for intrinsic constituents. the membrane proved to be chemically complex but not unique, consisting mainly of protein (52%), amino and neutral polysaccharides (20%), lipids (18%), and ash (4%). seventeen common amino acids were identified by chromatography, and were present in usual proportions except for low levels of cystine-cysteine, methionine, tyrosine, and histidine. ...19704983648
symposium on bacterial spores: x. heat resistance of spore enzymes. 19704986699
symposium on bacterial spores: i. cytology of spore formation and germination. 19704986702
ribosomes from spores of bacillus cereus t.data from analytical ultracentrifuge studies suggest that polymers of ribosomes exist in dormant spores of bacillus cereus t.19704985933
possible involvement of beta-lactamase in sporulation in bacillus cereus.nonreverting beta-lactamase-negative strains were isolated from the beta-lactamase-constitutive strain, bacillus cereus 569 h. these strains differed from both beta-lactamase-inducible and -constitutive strains not only in failure to produce beta-lactamase but also in failure to autolyze on aging, delayed sporulation, and failure to release free spores from sporangia when produced. the addition of b. cereus beta-lactamase of 15% purity to a final concentration of 10 iu/ml stimulates sporulation ...19704985546
[automation in microbiological analysis: determination of tetracycline by means of b. cereus and of vitamin b 12 means of l. leichmannii]. 19704989014
regulation of penicillinase synthesis: evidence for a unified model.the kinetics of penicillinase induction in bacillus cereus 569 was investigated. an increase in the rate of penicillinase synthesis was demonstrated within 30 sec of the addition of inducer (benzylpenicillin); however, the maximum induced rate of penicillinase synthesis was not attained until at least 30 min after the addition of inducer. in contrast to earlier claims, a quantitative estimate showed that the penicillinase messenger ribonucleic acid (mrna) half-life is approximately 2 min. these ...19704983646
microbioassay of antimicrobial agents.a previously described agar-diffusion technique for microbioassay of antimicrobial agents has been modified to increase sensitivity of the technique and to extend the range of antimicrobial agents to which it is applicable. this microtechnique requires only 0.02 ml of an unknown test sample for assay, and is capable of measuring minute concentrations of antibiotics in buffer, serum, and urine. in some cases, up to a 20-fold increase in sensitivity is gained relative to other published standardiz ...19704986725
macromolecular synthesis during microcycle sporogenesis of bacillus cereus t.microcycle sporogenesis induced in bacillus cereus t by phosphate limitation occurs over a narrow range of phosphate to spore inoculum ratios. sufficient phosphate is required to satisfy the demands for a twofold increase in deoxyribonucleic acid; net ribonucleic acid synthesis is not required. the total ribonucleic acid content of the culture was variable, and deoxyribonucleic acid synthesis was restricted to a twofold increase. developmental changes during outgrowth occurred synchronously, whe ...19704983651
derepression of beta-lactamase (penicillinase in bacillus cereus by peptidoglycans.in bacillus cereus 569 a cellular inducer of beta-lactamase was isolated which has the same constituents and basic structure as the soluble peptidoglycan found in sporulation, extracts from spores, and germination extracts, and which was previously called "spore-peptide." the material has been extensively purified and characterized. two acid-soluble, high-molecular-weight peptidoglycan fractions containing muramic acid, glucosamine, diaminopimelic acid, d-aspartate, and d- and l-alanine, -lysine ...19704985545
lipid composition of bacillus cereus during growth and sporulation.the lipid composition of bacillus cereus during growth and sporulation was examined. the total lipid extract accounted for 2 to 3% of the dry weight of the cells and consisted of neutral lipids (30 to 40%) and phospholipids (60 to 70%). phospholipids were separated by thin-layer chromatography into eight components; phosphatidyl ethanolamine, phosphatidyl glycerol, and diphosphatidyl glycerol were the major phospholipids and accounted for over 90% of the total. also identified was a diglycosyl d ...19704984075
activity of bacillus cerus proteinases in milk. 19704985290
transduction in bacillus cereus by each of two bacteriophages.the ability of phage cp-51 to mediate transduction both homologously and heterologously in some of its hosts was investigated. cp-51 was shown to transduce bacillus cereus strains 6464, 9139, and t in addition to 569 which was reported earlier from this laboratory. furthermore, cp-51 grown on b. thuringiensis was shown to transduce some mutants of b. cereus. during this investigation, a second transducing phage for b. cereus 569 was isolated from lysates of phage cp-51 grown on b. cereus 6464. t ...19704986764
iodine sensitivity of bacillus cereus penicillinase. 19704984552
the effect of copper on the radiosensitivity of bacteria. 19704984594
[differentiation of aerobic spore-forming bacteria with special reference to bacillus anthracis and bacullus thuringiensis]. 19704989311
ion-exchange separation and automated assay of complex mixtures of amino acids and hexosamines. 19704985912
differentiation between heat resistance and octyl alcohol resistance of the cells of bacillus cereus t. 19704984937
cytochromes in a cyanide-resistant strain of bacillus cereus. 19704329720
arrangement of fatty acyl groups in phosphatidylethanolamine from a fatty acid auxotroph of escherichia coli. 19704324094
[role of toxins of several saprophytic microbes in the etiology of experimental neuroparalytic diseases]. 19704394515
interference of barbiturates with pyrimidine incorporation. ii. structural specificity of the inhibition of orotate uptake in bacillus cereus. 19704398023
some regulatory properties of cytidine deaminase and uridine phosphorylase of bacillus cereus. 197011945358
effect of ultraviolet on the survival of bacteria airborne in simulated martian dust clouds.a chamber was constructed to create simulated martian dust storms and thereby study the survival of airborne micro-organisms while exposed to the rigors of the martian environment, including ultraviolet irradiation. representative types of sporeforming and non-sporeforming bacteria present in spacecraft assembly areas and indigenous to humans were studied. it was found that daily ultraviolet irradiation of 2 to 9 x 10(7) erg cm-2 was not sufficient to sterilize the dust clouds. the soil particle ...197012664918
some regulatory properties of purine nucleoside phosphorylase of bacillus cereus. 197111946156
characteristics of a bacteriocin derived from streptococcus faecalis var. zymogenes antagonistic to diplococcus peumoniae.a bacteriocin-producing strain of streptococcus faecalis var. zymogenes (e-1) was isolated from clinical material (conjunctiva). the active substance differed from bacteriocins described by other investigators primarily in its spectrum of antibacterial activity, especially by its marked inhibition of diplococcus pneumoniae. the e-1 bacteriocin also inhibited nonhemolytic strains of enterococci as well as one-third of the viridans group of streptococcal strains investigated. the degree of inhibit ...19714398532
a disulfide reductase in spores of bacillus cereus t. 19714399821
the function and evolution of penicillinase. 19714401417
[purification of guanosine phosphorylase of bacillus cereus]. 19714362339
chemical nature of the inactivation of bacillus cereus penicillinase by iodine. 19714327042
sarcoplasmic reticulum. xi. the mode of involvement of phospholipids in the hydrolysis of atp by sarcoplasmic reticulum membranes. 19714328159
silica gel stimulates the hydrolysis of lecithin by phospholipase a. 19714331782
[spores of microorganisms. xxiv. effect of derivatives of basic antibiotic peptides on the postgerminative development of bacterial spores]. 19714336956
enumeration and identification of bacillus cereus in foods. i. 24-hour presumptive test medium.an egg yolk-polymyxin medium (kg) for rapid enumeration of bacillus cereus is described. the test is presumptive in that differentiation of b. cereus (and closely related organisms) from other species is based on the formation of turbidity in the agar surrounding the colonies of the cereus group organisms. the medium is formulated to encourage sporulation and release of free spores for serological confirmatory tests within the 24-hr incubation period. the production of turbidity in egg yolk and ...19714331768
ultrastructural changes associated with activation and germination of bacillus cereus t spores.the ultrastructural changes occurring during defined stages of the transition of dormant bacillus cereus t spores into heat-sensitive forms were investigated. the coat of the heat-activated spores displayed conspicuous striations across its middle layer. electron microscopy of thin sections of heat-activated spores revealed the presence in the spore of a layer consisting of hexagonally oriented subunits. it was demonstrated that the subcoat region, but not the cortex, disappears rapidly during g ...19714993322
reduction of activity of reduced nicotinamide adenine dinucleotide oxidase by divalent cations in cell-free extracts of bacillus cereus t.a rapid and effective method was devised for the reduction of activity of reduced nicotinamide adenine dinucleotide (nadh) oxidase in crude extracts of bacillus cereus t. the addition of 25 mumoles of mncl(2) per mg of extract protein in tris(hydroxymethyl)aminomethane-hydrochloride buffer reduced nadh oxidase activity by 90% within 1 min, and this reduction was independent of ph between ph 7.0 and 8.5. other divalent cations such as mg(2+), ba(2+), ca(2+), and co(2+) also reduced nadh oxidase a ...19714993337
peptidoglycan differences in strains of bacillus cereus constitutive and inducible for penicillinase production.strains of bacillus cereus differing in penicillinase production were shown to possess cell walls of differing muramic acid contents.19714993342
amino acid composition and peptide maps of bacillus cereus 569-h penicillinase. 19714996025
bacillus cereus pneumonia and bacteremia. a case report. 19714996269
[concentration and separation of bacteria on dowex anionite]. 19714996294
characteristics of cir (cell injuring reaction) active substance in culture fluid of bacillus cereus, "ms-1063 strain". 19714996372
[some data on somatic antigens of bac. anthracis]. 19714996428
enhancement of bacillus cereus spore lytic enzyme by a heat-labile non-dialyzable factor in spore extracts. 19714996439
partial purification, specificity and mechanism of action of the nisin-inactivating enzyme from bacillus cereus. 19714994514
[interaction of penicillinases with 6-aminopenicillanic derrivatives of furan-2-carbonic and furyl-2-acrylic acids]. 19714994896
evidence for a magnesium pump in bacillus cereus t.unlike escherichia coli, bacillus cereus t appears to accumulate mg(2+) in its cell sap against a concentration gradient. over a range of mg(2+) in the growth medium from 5 x 10(-5) to 1.35 x 10(-2)m, the concentration of mg(2+) in the cell sap of b. cereus t was maintained at about 6 x 10(-3)m, and ribosome-bound mg(2+) and spermidine, as well as the spermidine concentration in the cell sap, appear to be unaffected by the concentration of mg(2+) in the growth medium. inhibition of growth of e. ...19714993319
physical and catalytic properties of the purine nucleoside phosphorylases from cells and spores of bacillus cereus t. 19714993482
isolation of glucosaminyl-beta(1-4)-muramic acid and phosphoric acid ester of this disaccharide from acid hydrolysates of peptidoglycan of bacillus cereus ahu 1356 cell walls. 19714993453
occurrence of non-n-substituted glucosamine residues in lysozyme-resistant peptidoglycan from bacillus cereus cell walls. 19714993454
sequence of events during rapid germination of spores of bacillus cereus. 19714996778
diglucosyldiglyceride from b. cereus. 19714993436
[contamination of foodstuffs and objects of the external environment with bac. cereus at public catering establishments]. 19714996923
immunochemical properties of polysaccharide fractions of bacillus cereus strains isolated from cases of food poisoning. 19714996969
the antimicrobial activity of citral. 19715002370
[biological properties of bacillus cereus phages]. 19714997859
in vivo and in vitro action of new antibiotics interfering with the utilization of n-acetyl-glucosamine-n-acetyl-muramyl-pentapeptide.recent literature on the antibiotics enduracidin, moenomycin, prasinomycin, and 11.837 rp suggested an interaction with murein synthesis. incubation of sensitive strains from bacillus cereus and staphylococcus aureus in a "wall medium" containing labeled l-alanine showed that all four antibiotics inhibited the incorporation of alanine into murein and gave rise to accumulation of radioactive uridine diphosphate-n-acetyl-muramyl (udp-murnac)-pentapeptide. peptidoglycan was synthesized when the par ...19715001198
microstructure of colonies of rod-shaped bacteria.whole colonies of bacillus cereus, b. megaterium, b. mycoides cn2495, corynebacterium hofmanni nctc1938, escherichia coli, lactobacillus acidophilus ncib1899, nocardia graminis nctc4728, pseudomonas viscosa, and serratia marcescens were prepared for scanning electron microscopic examination by freeze-drying and metal-coating. the arrangement of individual cells within colonies could be seen. cells of bacillus colonies tended to be longer than in liquid culture and irregular in shape and to give ...19715001203
molecular species of lecithins from various animal tissues. 19714995266
the interconvertibility of various bacterial transfer ribonucleic acids between an active and an inactive stable configuration. 19715000606
control of diaminopimelate decarboxylase by l-lysine during growth and sporulation of bacilluscereus.l-lysine caused repression of diaminopimelate decarboxylase synthesis in bacillus cereus when grown in either a minimal defined medium (cdgs medium) or a complex defined medium (a modified lysine assay medium). when cells were grown in either of the two media, variations in the specific activity of the enzyme as a function of time were found to be correlated with the intracellular lysine pool size during growth. from all of the data presented, it seems reasonable to conclude that during growth t ...19714995650
factors affecting the relative ratio of fatty acids in bacillus cereus. 19714994093
initiation of spore germination in bacillus cereus: a proposed allosteric receptor. 19714999536
[isolation and some properties of bacillus cereus toxin]. 19714999022
pseudogermination in dipicolinic acid-less spores of a bacillus cereus t mutant.dipicolinic acid-less spores of a bacillus cereus t mutant in 33 mm sodium bicarbonate (ph 8.5) underwent pseudogermination (germination-like changes) accompanied by ribonucleic acid leakage and loss of viability.19714994601
effects of glutamic acid on sporulation of bacillus cereus and on spore properties. 19714994922
[characteristics of the lysogeny and temperate phages of cultures of spore-bearing crystal-forming entomopathogenic microorganisms isolated from various sources]. 19714998585
a rapid method for removal of zinc from the metallo neutral proteases. 19714998623
[action of ionizing radiations on microorganisms and radiation sterilization (review)]. 19714997876
agar diffusion method for the differentiation of bacillus anthracis.a method was developed for identification of bacillus anthracis based on elaboration of protective antigen by individual colonies and its detection by double-diffusion precipitation in agar plates.19714993230
sporulation of a cortexless mutant of a variant of bacillus cereus.a stage 4 sporulation mutant of a strain of bacillus cereus var. alesti fails to synthesize a cortex although all other structural components appear normal. with terminal lysis the spore core as well as the sporangium is lysed. both the uptake of (45)ca and the synthesis of dipicolinic acid (dpa) are similar to these activities in the parent strain, but these components (dpa and ca) are lost to the medium with the drastic lysis. the first stage of diaminopimelic acid incorporation, that into ger ...19714993321
complete purification and some properties of phospholipase c from bacillus cereus. 19714997720
dielectric study of the physical state of electrolytes and water within bacillus cereus spores.dielectric measurements revealed that dormant spores of bacillus cereus have extremely low conductivities at high frequencies (50 mhz) and so must contain remarkably low concentrations of mobile ions both within the core and in the surrounding integuments. activation, germination, and outgrowth were all accompanied by increases in conductivity of the cells and their suspending medium, and this result indicated that intracellular electrolytes had become ionized and leaked from the spores. high-fr ...19714998245
spore refractility in variants of bacillus cereus treated with actinomycin d.refractility as indicated by light microscopy, electron microscopy of thin sections, and freeze fracture etching was increased and maintained in a cortexless mutant, a(-)1, of bacillus cereus var. alesti by the addition during sporulation stage 4 of actinomycin d, which prevents the terminal lysis of spore core associated with sporulation in this organism. (45)calcium uptake levels and dipicolinic acid (dpa) content were similarly maintained. the location of these components appears to be in the ...19714998247
uranyl salts as fixatives for electron microscopy. study of the membrane ultrastructure and phospholipid loss in bacilli. 19715000342
biochemical aspects of the visual process. 13. the role of phospholipids in cattle rhodopsin studied with phospholipase c. 19715001225
[mechanism of regulation of alpha-acetohydroxyacid-synthetase in bacillus cereus t]. 19715002914
effects of fluorouracil on rna synthesis in bacillus cereus. 19715002954
bacilli in milk. 2. sweet curd formation. 19714999494
effect of oxidation on the transition of penicillinase conformation. 19715001985
comparison of bacillus cereus bacteriophages cp-51 and cp-53.transducing bacteriophages cp-51 and cp-53 were compared. unlike cp-51, cp-53 appeared to be a lysogenizing phage. cp-51 gave greater frequencies of co-transduction for linked markers than did cp-53. cp-51 was found to be a larger phage which carried more deoxyribonucleic acid (dna) than cp-53. cp-51 dna contained about 43% guanine plus cytosine and in addition contained 5-hydroxymethyluracil in place of thymine. cp-53 dna contained no unusual bases; its guanine plus cytosine content was 37%.19715000543
spore removal by centrifugation and its effect on ultra-high temperature commercial sterilization of milk. 19714999495
[action of phospholipase c on diol phospholipids]. 19715001986
autolysis of bacillus cereus cell walls and isolation of structural components.autolysis of bacillus cereus n.r.r.l. 569 cell walls was accompanied by hydrolysis of the majority of the 4-o-beta-n-acetylglucosaminyl-n-acetylmuramic acid linkages in mucopeptide, presumably by an endo-beta-n-acetylglucosaminidase. hydrolysis of the n-acetylmuramyl-l-alanine linkages by an amidase also occurred. free d-alanine residues were detected in isolated cell walls and the proportion of these residues increased during autolysis, presumably due to d-alanine carboxypeptidase action. fract ...19715000275
reconstitution of bacterial spore coat layers in vitro.the spore coat layers of bacillus cereus t can be at least partially restored by mixing spores stripped of their coat with solubilized coat protein.19715001206
action of pure phospholipase a 2 and phospholipase c on human erythrocytes and ghosts. 19715003696
the effect of substrate analogues on the iodine sensitivity of penicillinase from b. cereus. 19715003710
effect of electrolytes on the activity and iodine sensitivity of penicillinase from b. cereus. 19715003711
the interrelation of polar lipids in bacterial membranes. 19715002559
[montreal episode of food poisoning due to bacillus cereus]. 19715003957
lung tissue and serum levels of methacycline. 19715002640
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