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l-lactate oxidase and l-lactate monooxygenase: mechanistic variations on a common structural theme.properties of l-lactate oxidase from aerococcus viridans are described. the gene encoding the enzyme has been isolated. from its cdna sequence the amino acid sequence has been derived and shown to have high similarity with those of other enzymes catalyzing oxidation of l-alpha-hydroxy acids, including flavocytochrome b2, lactate monooxygenase, glycolate oxidase, mandelate dehydrogenases and a long chain alpha-hydroxy acid oxidase. the enzyme is expressed in escherichia coli, and is a flavoprotei ...19958589073
penicillin-resistant aerococcus viridans bacteremia in a child receiving prophylaxis for sickle-cell disease. 19968838213
conversion of l-lactate oxidase to a long chain alpha-hydroxyacid oxidase by site-directed mutagenesis of alanine 95 to glycine.a mutant form of l-lactate oxidase (lox) from aerococcus viridans in which alanine 95 was replaced by glycine was constructed as a mimic of l-lactate monooxygenase but proved instead to be a mimic of the long chain alpha-hydroxyacid oxidase from rat kidney. a95g-lox keeps oxidase activity with l-lactate at the same level as wild type lox but has much enhanced oxidase activity with longer chain l-alpha-hydroxyacids, alpha-hydroxy-n-butyric acid, alpha-hydroxy-n-valeric acid, etc., and also the ar ...19968910450
[gram-positive, catalase negative cocci isolated from clinical material--identification and drug sensitivity of strains].4608 clinical specimens (including 1641 samples of blood) for bacteriological examination were obtained from patients hospitalized in different wards of national clinical hospital no 1 in warsaw. strains were cultured, identified and their sensitivity to antibacterial drugs was determined during 14 months with the use of semi-automated api (atb-plus) system (biomérieux, france). strains of gram-positive, catalase-negative cocci were separated from all cultured strains. they belonged to the gener ...19968927737
aerococcus-like organisms: use of antibiograms for diagnostic and taxonomic purposes.recently, some aerococcus-like organisms (alos), isolated from urine and blood of elderly patients with urinary tract infection, have been described. in this study alos and related taxons were tested for susceptibility by agar diffusion and agar dilution methods to 15 selected antimicrobial agents for diagnostic and taxonomic considerations. alos were susceptible to a wide range of antimicrobials including beta-lactams, but resistant to aminoglycosides, sulphonamides, trimethoprim and nalidixic ...19968877539
inhibition of peptidoglycan biosynthesis in vancomycin-susceptible and -resistant bacteria by a semisynthetic glycopeptide antibiotic.ly191145 is a p-chlorobenzyl derivative of ly264826 (a82846b) with activity against both vancomycin-susceptible and -resistant enterococci. incorporation of l-[14c]lysine into peptidoglycan of intact vancomycin-susceptible and -resistant enterococcus faecium was inhibited by ly191145 (50% inhibitory concentrations of 1 and 5 microgram/ml, respectively). inhibition was accompanied by accumulation of udp-muramyl-peptide precursors in the cytoplasm. this agent inhibited late-stage steps in peptidog ...19968891144
atmospheric growth requirements for alloiococcus species and related gram-positive cocci.the growth of alloiococcus otitis under different atmospheres and nutritional conditions was studied. the growth rates of 25 strains of gram-positive cocci representing five genera on heart infusion agar plates containing 5% rabbit blood and on brucella agar plates with and without sheep blood under aerobic, increased co2, and anaerobic atmospheres were compared. eight strains of alloiococci plated on heart infusion agar with rabbit blood and on brucella sheep blood agar grew under aerobic and c ...19968815077
in vitro antibacterial activity of ly333328, a new semisynthetic glycopeptide.ly333328 is a semisynthetic n-alkyl derivative of ly264826, a naturally occurring structural analog of vancomycin. ly333328 was evaluated for its in vitro inhibitory and bactericidal activities in comparison with those of the two currently available glycopeptides (vancomycin and teicoplanin). glycopeptide-susceptible test strains included a total of 311 isolates (most of clinical origin) from the genera staphylococcus, enterococcus, streptococcus, aerococcus, gemella, lactococcus, listeria, cory ...19979333042
urinary tract infections with aerococcus urinae in the south of the netherlands.aerococcus urinae is an uncommon urinary tract pathogen that causes infections predominantly in elderly persons with local or general predisposing conditions. during a one-year study, the clinical features of aerococcus urinae urinary tract infections (> or = 10(5) cfu/ml) were investigated in two large medical microbiology laboratories in the netherlands. the incidence of aerococcus urinae urinary tract infections ranged between 0.31 and 0.44% for the two laboratories. the median age (range 35- ...19979495666
aerococcus urinae: intraspecies genetic and phenotypic relatedness.a number of aerococcus-like organisms were recently recognized as human pathogens. five aerococcus-like strains were proposed as members of the new species aerococcus urinae (with type strain e2 [= nctc 12142]) on the basis of the results of a 16s rrna sequence analysis. the intraspecies phenotypic and genetic relatedness of 22 selected a. urinae strains was investigated, and a hitherto unrecognized esculin hydrolysis-positive biotype was identified. a total of 14 of the 15 more common esculin-n ...19978995798
l-lactate oxidase from aerococcus viridans crystallized as an octamer. preliminary x-ray studies.crystals of flavoenzyme l-lactate oxidase from aerococcus viridans (lox) have been obtained that diffract to 3.0 a resolution (p2(1)2(1)2(1), a = 118.4 a, b = 138.4 a, c = 194.6 a). crystallographic studies suggest that the enzyme may exist as an octameric form with non-crystallographic two- and four-fold axes in the center of the octamer. the four-fold axis makes the tetramer tight, and the tetramers lie upon one another by the two-fold axis.19989672750
enzyme characteristics of beta-d-galactosidase- and beta-d-glucuronidase-positive bacteria and their interference in rapid methods for detection of waterborne coliforms and escherichia coli.bacteria which were beta-d-galactosidase and beta-d-glucuronidase positive or expressed only one of these enzymes were isolated from environmental water samples. the enzymatic activity of these bacteria was measured in 25-min assays by using the fluorogenic substrates 4-methylumbelliferyl-beta-d-galactoside and 4-methylumbelliferyl-beta-d-glucuronide. the enzyme activity, enzyme induction, and enzyme temperature characteristics of target and nontarget bacteria in assays aimed at detecting colifo ...19989501441
airways microbial flora in copd patients in stable clinical conditions and during exacerbations: a bronchoscopic investigation.patients affected with chronic obstructive pulmonary disease (copd) undergo frequent exacerbations of their illness characterized by increased cough and expectoration. the precise aetiology of these episodes often remains unknown. in the absence of clinical or radiographic signs of pneumonia, bacterial or viral cultures of sputum usually provide little useful information. thus, we performed fibreoptic bronchoscopy using a protected specimen brush (psb) to obtain uncontaminated secretions for cul ...19989785808
[database on nucleotide sequences used as primers of microorganisms].the data base (db) "primers of microorganisms" for the accumulation and systematization of information on oligonucleotide sequences, used as primers in polymerase chain reaction, has been created. this db includes data on primers for the laboratory diagnostics of 20 bacterial genera (aerococcus, aeromonas, bartonella, borrelia, burkholderia, chlamydia, clostridium, corynebacterium, escherichia, francisella, helicobacter, legionella, listeria, mycobacterium, mycoplasma, salmonella, shigella, stap ...19989825498
[a biological model study of the effect of aerococcus viridans on pathogenic bacteria].morphological researches studies showed that the treatment of infected skin wounds with aerobact quickly eliminated inflammation, promoted regeneration of damaged tissues, that actively stimulated formation of granulations. a decrease of s. aureus virulence which completely or partially loose lethal and necrotic activity is one of the reasons of the observed phenomenon. on the model of s. typhimurium infection the expressed protective action of bacteria of aerococcus genus was shown; this gives ...19989785800
intestinal protozoa and associated bacteria in captive houbara bustards (chlamydotis undulata) in the united arab emirates.a protozoal survey was carried out in 114 captive houbara bustards (chlamydotis undulata) in the united arab emirates. trichomonas gallinarum, chilomastix gallinarum, giardia spp. and lophomonas spp. were detected from faecal samples of 49 (43%) captive houbara bustards. culture and identification studies were carried out to assess associated bacteria. the bacteria isolated from birds negative to protozoa included enterococcus spp., aerococcus spp., micrococci spp., proteus spp., enterobacter sp ...199916147554
an automatic flow-injection analysis system for determining phosphate ion in river water using pyruvate oxidase g (from aerococcus viridans).an automated flow-injection system combining a pyruvate oxidase reaction and chemiluminescence for the detection of phosphate ion in river water has been developed. in this research, we used pyruvate oxidase g (pyroxg), from aerococcus viridans, immobilizing it on n-hydroxysuccinicacidimido beads without a cross-linker. in this sensor system, which was constructed as a trial system of desktop type, the temperature was precisely controlled. after the sensor system was optimized, a calibration cur ...199918967770
cloning and analysis of the l-lactate utilization genes from streptococcus iniae.the presence of lactate oxidase was examined in eight streptococcus species and some related species of bacteria. a clone (pgr002) was isolated from a genomic library of streptococcus iniae generated in escherichia coli, containing a dna fragment spanning two genes designated lcto and lctp. we show that these genes are likely to be involved in the l-lactic acid aerobic metabolism of this organism. this dna fragment has been sequenced and characterized. a comparison of the deduced amino acid sequ ...199910508058
molecular and physiological characterization of dominant bacterial populations in traditional mozzarella cheese processingthe development of the dominant bacterial populations during traditional mozzarella cheese production was investigated using physiological analyses and molecular techniques for strain typing and taxonomic identification. analysis of rapd fingerprints revealed that the dominant bacterial community was composed of 25 different biotypes, and the sequence analysis of 16s rdna demonstrated that the isolated strains belonged to leuconostoc mesenteroides subsp. mesenteroides, leuc. lactis, streptococcu ...199910583686
identification of aesculin-hydrolyzing streptococci, lactococci, aerococci and enterococci from subclinical intramammary infections in dairy cows.aesculin-hydrolyzing, catalase-negative, gram-positive cocci isolated from subclinical intramammary infections in dairy cows were identified to species level using growth characteristics and biochemical activity. the results indicated that the aesculin-hydrolyzing cocci associated with this type of infection are a very heterogenic group. s. uberis strains, including inulin- or beta-glucuronidase-negative isolates, accounted for only about one-third of the collection, and enterococcus faecalis st ...199910591500
the first reported case of aerococcus bacteremia in a patient with hiv infection. 199910603227
caries development and aerococcus viridans. 199910613146
septicaemia and endomyocarditis caused by aerococcus urinae.aerococcus urinae, an uncommon urinary tract pathogen, was recently shown to cause septicaemia and endocarditis in a few patients in denmark and the netherlands. in austria this is the first report of a fatal course of endomyocarditis by aerococcus urinae, associated with multiple septic infarcts.199910420497
[the effect of aerococcus viridans on the properties of salmonellae and staphylococci in vitro].according to electron microscopy the repeated contacts of aerococcus viridans 167 (industrial strain for the probiotic aerobact production) with salmonella typhimurium and staphylococcus aureus caused the deep changes of cellular structure, down to bacteriolysis. remaining viable pathogenic cells gave posterity with changed biochemical properties and virulence. s. typhimurium completely lost typical biochemical properties, its mobility and ability to be agglutinated by o-specific salmonella seru ...199910483228
three cases of serious infection caused by aerococcus urinae.three cases of serious infection caused by aerococcus urinae are presented: a patient with endocarditis and two patients with soft-tissue infection (phlegmon and balanitis respectively). the literature on aerococcus urinae infections is reviewed and the antibiotic therapy discussed. aerococcus urinae is a pathogen isolated primarily from urine specimens of elderly patients with local or systemic predisposing conditions. most infections are mild, but serious infections such as endocarditis and se ...199910421047
endocarditis due to aerococcus urinae: diagnostic tests, fatty acid composition and killing kinetics.two cases of aerococcus urinae endocarditis are reported. the organism is not included in any database of commercial identification systems at this time. formation of tetrades and positive reactions for leucine arylamidase and beta-glucuronidase pointed strongly to a. urinae. the cellular fatty acid pattern was similar to that of aerococcus viridans, with predominantly c16:0, c18:1 omega 9c and c18:0; the presence of c18:1 omega 7t differentiated our isolates from a. viridans and can support the ...199910219643
aerococcus christensenii sp. nov., from the human vagina.phenotypic and phylogenetic studies were performed on two strains of a hitherto undescribed aerococcus-like organism isolated from the human vagina. comparative 16s rrna gene sequencing studies demonstrated that the unknown strains constitute a new subline within the genus aerococcus. the unknown bacterium was readily distinguished from the two currently recognized aerococcus species, aerococcus viridans and aerococcus urinae, by biochemical tests and electrophoretic analysis of whole-cell prote ...199910425770
[aerococcus urinae. a newcomer among pathogenic bacteria in clinical and microbiological practice].a decade ago we discovered a hitherto unrecognized aerococcus-like organism (alo) in urine specimens from patients with urinary tract infection. further microbiological and clinical investigations enabled us to classify it as a human pathogen also causing urogenic septicaemia and endocarditis, the latter often with lethal outcome. the organism is now designated aerococcus urinae.199910778337
development of bacterial contamination during production of yeast extracts.baker's yeast suspensions having bacterial populations of 10(6) and 10(8) cfu/ml were subjected to autolysis processes designed to obtain yeast extracts (ye). the bacterial contaminants added to the yeast cell suspensions were produced with spent broths obtained from a commercial yeast production plant and contained 59% cocci (leuconostoc, aerococcus, lactococcus) as well as 41% bacilli (bacillus). autolyses were conducted at four different ph levels (4.0, 5.5, 7.0, and 8.5) and with two autolys ...199910388734
on the interpretation of quantitative structure-function activity relationship data for lactate oxidase.the native flavin, fmn, has been removed from the l-lactate oxidase of aerococcus viridans, and the apoprotein reconstituted with 12 fmn derivatives with various substituents at the flavin 6- and 8-positions. impressive linear relationships are exhibited between the sum of the hammett final sigma(para) and final sigma(ortho) parameters and the redox potentials of the free flavins, and between the redox potentials of the free and enzyme-bound flavins. rapid reaction kinetics studies of the recons ...200010706608
aerococcus urinae in urinary tract infections.aerococcus urinae is a rarely reported pathogen, possibly due to difficulties in the identification of the organism. a. urinae is a gram-positive coccus that grows in pairs and clusters, produces alpha-hemolysis on blood agar, and is negative for catalase and pyrrolidonyl aminopeptidase. some of these characteristics and its being absent from the databases of most commercial identification systems could allow a. urinae to be misidentified as a streptococcus, enterococcus, or staphylococcus. we r ...200010747177
properties and stability of glycerophosphate oxidase isolated from a mutant strain of aerococcus viridans.the properties of microbial l-alpha-glycerophosphate oxidase (gpo) isolated from a mutant strain of aerococcus viridans dbm 1509 were estimated. the stability at different temperatures and ph were detected. at 4 degrees c the enzyme lost activity during 15 d, at 20 degrees c and 30 degrees c gpo activity decreased during 30 and 25 h, respectively. the highest stability was measured at - 20 degrees c and ph 9. at 4 degrees c the stability was enhanced by the addition of 0.1 m edta or by lyophiliz ...200010747248
serological properties of abiotrophia and granulicatella species (nutritionally variant streptococci).serological variations were examined among 12 type or reference strains and 91 oral isolates of vitamin b6-dependent abiotrophia and granulicatella spp. rabbits were immunized with whole cells of 12 selected strains and 10 typing antisera were obtained, which were unreactive with the lancefield group a to g antigen preparations. the reactivity of the antisera and autoclaved cell surface antigen extracts was tested by double diffusion in agar gel and a capillary precipitin test. these typing anti ...200011220686
interaction of two arginine residues in lactate oxidase with the enzyme flavin: conversion of fmn to 8-formyl-fmn.two arginine residues, arg-181 and arg-268, are conserved throughout the known family of fmn-containing enzymes that catalyze the oxidation of alpha-hydroxyacids. in the lactate oxidase from aerococcus viridans, these residues have been changed to lysine in two single mutations and in a double mutant form. in addition, arg-181 has been replaced by methionine to determine the effect of removing the positive charge on the residue. the effects of these replacements on the kinetic and thermodynamic ...200011078532
isolation and identification of bacteria associated with adult laboratory mexican fruit flies, anastrepha ludens (diptera: tephritidae).from the guts of new and old colonies (female and male) of mexican fruit flies, anastrepha ludens (diptera: tephritidae), we identified a total of 18 different bacterial species belonging to the family enterobacteriaceae, pseudomonadaceae, vibrionaceae, micrococcaceae, deinococcacea, bacillaceae, and the genus listeria. enterobacter, providencia, serratia, and staphylococcus spp. were the most frequently isolated genera, with citrobacter, streptococcus, aerococcus, and listeria found less freque ...200111178731
alkalibacterium olivoapovliticus gen. nov., sp. nov., a new obligately alkaliphilic bacterium isolated from edible-olive wash-waters.a novel gram-positive, obligately alkaliphilic, non-sporulating, rod-shaped, flagellated bacterium is described. three different strains of the bacterium were isolated from the wash-waters of edible-olive production. the strains are motile, psychrotolerant, halotolerant, facultatively anaerobic bacteria with a ph optimum of 9.0-9.4 for two strains and 9.8-10.2 for the third. they are catalase- and oxidase-negative. a range of hexoses and some disaccharides composed of hexoses, but not pentoses a ...200111414268
bacterial flora of the conjunctiva and nasal cavity in normal and diseased captive bustards.a survey was carried out to describe the normal aerobic bacterial flora of the conjunctiva and nasal cavity of captive houbara bustards (chlamydotis undulata), kori bustards (ardeotis kori), and white-bellied bustards (eupodotis senegalensis) maintained at the national avian research center, abu dhabi, united arab emirates. a total of 58 samples were examined from the nasal cavity and 55 samples from the conjunctiva of healthy bustards. there was no bacterial growth in 45% of conjunctival sample ...200111417826
detection of serum thermolabile beta-2 macroglycoprotein (hakata antigen) by enzyme-linked immunosorbent assay using polysaccharide produced by aerococcus viridans.although a serum thermolabile beta-2 macroglycoprotein (tmg) may play a role in host defense as a lectin, little is known of its related physiological functions, mainly due to a lack of appropriate methods for tracing the functions of tmg. we identified a polysaccharide from aerococcus viridans, psa, which reacts with tmg, and based on this finding, we developed an enzyme-linked immunosorbent assay to trace the functions of tmg. using ethanol precipitation and deae-sepharose and sephacryl s-400 ...200111238239
in vitro antimicrobial susceptibility of aerococcus urinae to 14 antibiotics, and time-kill curves for penicillin, gentamicin and vancomycin.aerococcus urinae is a newcomer in clinical and microbiological practice, causing urinary tract infections, bacteraemia/septicaemia and/or endocarditis. this study presents for the first time an evaluation of the activity of a representative panel of antibiotics against a large number of a. urinae isolates. the in vitro susceptibilities (mics) of 56 isolates of a. urinae to 14 antibiotics were determined by agar dilution. in general, a. urinae isolates showed little inter-isolate variability, an ...200111679554
estimated prevalence of aerococcus viridans and anophryoides haemophila in american lobsters homarus americanus freshly captured in the waters of prince edward island, canada.the canadian lobster industry holds lobsters homarus americanus in captivity for various periods to supply markets with live product year-round. mortality during holding results in considerable losses, estimated at 10 to 15 % yr(-1) by the industry. this study examined the prevalence of anophryoides haemophila and aerococcus viridans, causative agents of 'bumper car' disease and gaffkemia, respectively, in lobsters freshly captured in the waters of prince edward island during the spring and fall ...200111710558
highly efficient aerococcus viridans l-alpha-glycerophosphate oxidase production in the presence of h2o2-decomposing agent: purification and kinetic characterization.glycerophosphate oxidase was purified from aerococcus viridans cells by phase partitioning in triton x-114, ammonium sulfate fractionation, fplc ion-exchange chromatography and fplc hydrophobic-interaction chromatography. the purification achieved from a crude extract of a. viridans was 38-fold with a 32% recovery of activity. under the growth conditions used, a. viridans strain cect 978 proved to be an excellent glycerophosphate-oxidase producer, with enzyme production 2,800-fold greater than t ...200111759680
dna relatedness, phenotypic characteristics, and antimicrobial susceptibilities of globicatella sanguinis strains.dna-dna reassociation was performed on 15 strains of globicatella sanguinis to compare their taxonomic status with phenotypic characterization. all 15 strains selected for dna-dna reassociation readily met the criteria for species relatedness. the relative binding ratio was 81% or greater at the optimal temperature and 76% or greater at the stringent temperature, and the divergence was less than 3% for all strains hybridized with the type strain. these strains included nine strains from the cent ...200111682529
non-linear slow-binding inhibition of aerococcus viridans lactate oxidase by cibacron blue 3ga.lactate oxidase (lod) was purified from cells of aerococcus viridans by phase partitioning in triton x-114 (tx-114), ammonium sulphate fractionation and fplc ion exchange chromatography. the purification achieved from a crude extract of a. viridans was 32-fold with a 60% recovery of activity. the isolated enzyme was a true fmn-containing lod in tetrameric form with a subunit molecular weight of 48,000. the km for l-lactate was 175 microm, a 6-fold less value than described in the literature. amo ...200111916135
aerococcus urinaehominis sp. nov., isolated from human urine.phenotypic and phylogenetic studies were performed on an unknown gram-positive catalase-negative coccus isolated from human urine. comparative 16s rrna gene sequencing demonstrated that the organism represents a new subline within the genus aerococcus. the unknown bacterium was readily distinguished from the three currently recognized aerococcus species, aerococcus christensenii, aerococcus urinae and aerococcus viridans, by biochemical tests and electrophoretic analysis of whole-cell proteins. ...200111321115
aerococcus sanguicola sp. nov., isolated from a human clinical source.phenotypic and phylogenetic studies were performed on an unknown, gram-positive, catalase-negative coccus isolated from human blood. comparative 16s rrna gene sequencing demonstrated that the organism represents a new subline within the genus aerococcus. the unknown bacterium was readily distinguished from the three currently recognized aerococcus species, aerococcus christensenii, aerococcus urinae and aerococcus viridans, by biochemical tests and electrophoretic analysis of whole-cell proteins ...200111321093
isolation of hexavalent chromium-reducing cr-tolerant facultative anaerobes from tannery effluent.several facultative anaerobes tolerant to high levels of chromate (>400 mg/ml) were isolated from tannery effluents. these isolates displayed varying degrees of cr(vi) reduction under aerobic and anaerobic conditions at room temperature (24+/-2 degrees c). interestingly, eight isolates were efficient in reducing 70% cr(vi) anaerobically. this includes 5 isolates of genus aerococcus, two isolates of micrococcus and single isolate of genus aeromonas. these isolates were subjected to further charac ...200112483605
microencapsulation of aerococcus viridans with catalase and its application for the synthesis of dihydroxyacetone phosphate.dihydroxyacetone phosphate is essential for the synthesis of polyhydroxylated compounds used as components or precursors of active pharmaceutical substances, such as antibiotics or glycosidase inhibitors. dihydroxyacetone phosphate was produced by enzymatic oxidation of l-alpha-glycerophosphate in the presence of glycerophosphate oxidase or aerococcus viridans coimmobilized with a hydrogen peroxide-decomposing enzyme. the microencapsulation of a. viridans with catalase in sodium alginate showed ...200211831477
esophageal diverticulum associated with aerococcus viridans infection in a loggerhead sea turtle (caretta caretta).a juvenile loggerhead sea turtle (caretta caretta) stranded in gran canaria, spain was necropsied. the turtle was underweight, had sunken eyes, and small amounts of crude oil were in the oral cavity. the most significant lesion was a large esophageal diverticulum at the junction of the esophagus and stomach. the diverticulum was full of gas and green mucoid fluid and a diffuse thick yellow fibrinonecrotic membrane covered the mucosa. the lumen of the diverticulum also contained moderate numbers ...200211838222
penicillin-resistant aerococcus viridans bacteremia associated with granulocytopenia.aerococcus viridans, a catalase-negative gram-positive coccus rarely causing bacteremia, was isolated from blood cultures of a 52-yr-old man under the granulocytopenic condition. the isolate showed the typical characteristics of a. viridans, i.e., tetrad arrangements in gram stain, positive pyrrolidonyl aminopeptidase (pyr) and negative leucine aminopeptidase (lap) reactions, and no growth at 45 degrees c. the isolate was revealed to be highly resistant to penicillin, erythromycin, clindamycin, ...200211850599
[hemopericardium caused by endocarditis ulcerosa: an unusual cause of sudden death in adolescence].the authors report on the case of a 18-year-old student who died suddenly and unexpectedly of a pericardial tamponade after perforated ulcer-polypous endocarditis of the aortic valve. the detection of aerococcus urinae--which usually causes urinary tract infection--on the inflamed aortic valve demonstrates an unusual pathogenetic chain linking complexes of findings in the urogenital system and the heart.200212532678
use of dye affinity chromatography for the purification of aerococcus viridans lactate oxidase.lactate oxidase was purified from aerococcus viridans (a. viridans) by dye affinity chromatography and fplc ion exchange chromatography. the lactate oxidase could be purified by comparatively simple procedures, the purification achieved from a crude extract of a. viridans was 41-fold with a specific activity of 143 units/(mg of protein). the purified enzyme was a l-lactate oxidase, which catalyses the conversion of l-lactate in the presence of molecular oxygen to pyruvate and h(2)o(2). this puri ...200212052088
activation of the lectin complement pathway by h-ficolin (hakata antigen).ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. in human serum, there are two types of ficolins named l-ficolin/p35 and h-ficolin (hakata ag), both of which have lectin activity. we recently demonstrated that l-ficolin/p35 is associated with mannose-binding lectin (mbl)-associated serine proteases (masp) 1 and 2 and small mbl-associated protein (smap), and that the complex activates the lectin pathway. in this study, we report the characteri ...200211907111
identification of mouse submaxillary gland protein in mouse saliva and its binding to mouse oral bacteria.the mouse submaxillary gland protein (msmgp) is highly expressed in the submandibular gland of the adult mouse and rat. it shares 51% identity at the amino-acid level with a human protein, the prolactin-inducible protein (pip)/gross cystic disease fluid protein 15 (gcdfp-15), which has been found in saliva, tears, sweat, seminal plasma, submucosal glands of the lung and amniotic fluid. more recently, the human pip has been reported to bind to bacterial strains normally found in the mouth, ear ca ...200211922875
carnobacterium viridans sp. nov., an alkaliphilic, facultative anaerobe isolated from refrigerated, vacuum-packed bologna sausage.a facultatively anaerobic, non-spore-forming, psychrophilic, gram-positive, non-aciduric but alkaliphilic, rod-shaped bacterium (mpl-11t) was found to be responsible for green discoloration of refrigerated vacuum-packaged bologna upon opening of the package. although aerococcus viridans, which had been implicated earlier in causing the same problem, was also found, this is the first report of discoloration caused by an organism shown to be a species of carnobacterium. bacterial discoloration was ...200212361300
superior vena cava syndrome caused by aerococcus viridans para-aortic abscess after heart transplantation.superior vena cava (svc) syndrome is usually caused by extrinsic compression by malignant tumors, but para-aortic abscess can be responsible for this syndrome. we report a case of svc syndrome caused by para-aortic abscess caused by aerococcus viridans infection in a man who received orthotopic heart transplantation. a. viridans is a common saprophytic organism in hospital environments. there are reports of this organism being the cause of endocarditis, but no reports of it as the cause of a par ...200212451252
serum concentration of hakata antigen, a member of the ficolins, is linked with inhibition of aerococcus viridans growth.hakata antigen (hakata) is a novel serum glycoprotein that consists of collagen- and fibrinogen-like domains, similar to ficolin/p35. our research suggested that serum hakata may be a target of a polysaccharide (psa) produced by aerococcus viridans.200212367778
aerococcus urinae endocarditis: case report and review of the literature.aerococcus urinae is a rare cause of urinary tract infections, mainly in elderly men with underlying urinary tract pathologies. in addition, it has been described as a pathogen in balanitis, soft tissue infections, septicemia and endocarditis. to date ten cases of a. urinae endocarditis have been reported in the literature with a high rate of mortality (7/10) and morbidity, as two out of three survivors suffered from neurovascular complications. here we present the case of an additional patient ...200212382093
evaluation of five selective media for isolation of catalase-negative gram-positive cocci from bulk tank milk.five selective media including edwards modified medium, edwards modified medium supplemented with colistin sulfate (5 mg/l) and oxolinic acid (2.5 mg/l), streptococcus selective medium, streptosel agar, and thallium-crystal violet-toxin-ferric citrate medium were evaluated for the isolation of streptococci and streptococci-like organisms from raw milk. the sensitivity and specificity of these selective media for streptococci and streptococci-like organisms were determined by using american type ...200212086047
the response of the lymphatic system to the human skin resident bacteria.we designed a study to identify skin bacterial strains producing most intensive changes in lymphoid tissue of the draining nodes and analyse the node cell phenotypes reacting to bacterial antigens. the obtained results were expected to help in establishing rational therapy in recipients of hand allografts. the highest level of activation of lymphoid tissue was found after inoculation with s. epidermidis, acinetobacter, enterobacter and aerococcus. there was an increase in weight and cell concent ...200212854344
antimicrobial activity of a novel des-fluoro (6) quinolone, garenoxacin (bms-284756), compared to other quinolones, against clinical isolates from cancer patients.the in vitro spectrum of a novel des-fluoro (6) quinolone, garenoxacin (bms-284756), was compared with that of ciprofloxacin, levofloxacin, and trovafloxacin against 736 clinical isolates from cancer patients. garenoxacin was the most active agent overall against gram-positive organisms, with potent activity against aerococcus spp., micrococcus spp., rhodococcus equi, stomatococcus mucilaginous, bacillus spp., enterococcus faecalis, listeria monocytogenes, methicillin-susceptible staphylococcus ...200212458127
in vitro evaluation of azd2563, a new oxazolidinone, tested against unusual gram-positive species.the recently introduced oxazolidinone, linezolid, has a spectrum and potency directed against gram-positive organisms, including antimicrobial-resistant isolates. the newest agent in this class, azd2563 was tested against uncommonly isolated gram-positive species to establish the breadth of its spectrum. by reference broth microdilution methods, 120 strains were tested (48 corynebacterium spp., 10 species; 27 listeria spp., 2 species; 11 micrococcus spp., 2 species; 23 bacillus spp., 3 species; ...200211858907
[aerococcus urinae and urinary tract infection].bacteriological investigation of urine often shows the presence of alpha-haemolytic streptococci, but the clinical significance of this finding may be unclear. among the alpha-haemolytic streptococci found in urine is aerococcus urinae, which is pathogenic.200211873573
lymphadenitis caused by aerococcus urinae infection.aerococcus urinae is rarely reported as a human pathogen. the pathogenesis of this gram-positive coccus is not fully understood. it has been identified as a cause of urinary tract infections but may be associated with severe infections such as endocarditis and septicaemia. this paper presents a case of lymphadenitis caused by a. urinae.200312875530
high-level gentamicin-resistant isolates of oral streptococci and aerococcus from blood specimens.seventy-nine isolates of oral streptococci and aerococcus obtained from blood specimens between december 1997 and january 2000 were tested for high-level gentamicin resistance. three isolates with minimum inhibitory concentrations (mics) of > or =1024 microg/ml were selected as potential high-level gentamicin-resistant isolates. the dna sequence of the structural gene encoding 6'-acetyltransferase-2"-phosphotransferase in the three high-level gentamicin-resistant isolates was investigated accord ...200312673402
[urinary infection by aerococcus urinae]. 200312765596
spondylodiscitis due to aerococcus urinae: a first report.aerococcus urinae is an uncommon urinary tract pathogen which causes infections predominantly in elderly persons with local and general predisposing conditions. there are few case reports of severe infections caused by aerococcus urinae among which only 14 cases of endocarditis caused by aerococcus urinae have been reported. we report the first case of aerococcus urinae spondylodiscitis. the patient responded to 4 weeks of amoxicillin and clindamycin, and to 5 months of amoxicillin alone.200314723372
macrolide and lincosamide resistance in the gram-positive nasal and tonsillar flora of pigs.macrolide and lincosamide resistance phenotypes and the presence of the erm(a), erm(b), erm(c), and mef(a) genes were determined in 344 bacterial strains belonging to 34 species and nine genera, isolated from the tonsils and nasal cavities of 2-week- and 6-week-old piglets, derived from four different farms. these piglets had never before been treated with macrolides or lincosamides. macrolide and lincosamide resistance was most frequently present in streptococcus and enterococcus strains, of wh ...200312959408
in vitro antimicrobial activity of moxifloxacin compared to other quinolones against recent clinical bacterial isolates from hospitalized and community-based cancer patients.the in vitro spectrum of moxifloxacin (a c-8-methoxyquinolone) was compared to that of ciprofloxacin and levofloxacin against 924 recent clinical isolates from cancer patients. moxifloxacin was more active than the comparator agents against gram-positive pathogens, with potent activity against aerococcus spp., listeria monocytogenes, micrococcus spp., rhodococcus equi, and stomatococcus mucilaginous, methicillin-susceptible staphylococcus spp., all beta hemolytic streptococci, viridans streptoco ...200314522520
a study on the kinetic mechanism of apoenzyme reconstitution from aerococcus viridans lactate oxidase.the preparation of a reconstitutable apoprotein is widely recognized as an important tool for studying the interactions between protein and coenzyme and also for characterizing the coenzyme-binding site of the protein. here is described the kinetic analysis of the reconstitution of aerococcus viridans lactate oxidase apoenzyme with fmn and fad in the presence of substrate. the reconstitution was followed by measuring the increase in catalytic capacity with time. lactate oxidase activity was easi ...200314506921
identification of lactic acid bacteria from spontaneous fermentation of 'almagro' eggplants by sds-page whole cell protein fingerprinting.in order to complete the previously performed phenotypic identification [sánchez et al., 2000. int. j. food microbiol. 59 (2000) 9], whole cell protein patterns obtained using sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) of 149 lactic acid bacteria (lab) strains, isolated from 'almagro' eggplants spontaneous fermentation, were analysed. phenotypic identification of those strains had revealed the presence of the species lactobacillus (lb) plantarum, lb. pentosus, lb. brev ...200312568758
phenotypic description and antimicrobial susceptibilities of aerococcus sanguinicola isolates from human clinical samples.this report describes the clinical sources and phenotypic characterization of 16 isolates of aerococcus sanguinicola. sixteen conventional tests were used to describe and differentiate the 16 isolates of a. sanguinicola from 30 strains of aerococcus viridans, 27 strains of aerococcus urinae, and a single strain each of aerococcus christensenii and aerococcus urinaehominis. the phenotypic characterizations of the type strains for each species and 14 a. sanguinicola isolates were also compared in ...200312791884
thermal resistances and lactate and diacetate sensitivities of bacteria causing bologna discolouration.this report describes the effects of heat, sodium lactate, and sodium diacetate on the viabilities of weissella viridescens atcc 12706, aerococcus viridans mpl-1 and mpl-b, and carnobacterium viridans atcc baa 336. the latter three organisms were isolated from commercial product and have previously been shown to produce green discolourations in cooked cured bologna. w. viridescens was heat resistant in beef bologna (d(60 degrees c)=14.7 min) but not in apt broth. a. viridans and c. viridans were ...200312915033
identification of aerococcus urinae in urine samples.to evaluate procedures for the identification of aerococcus urinae, we examined 24 alpha-hemolytic non-enterococcal bacterial isolates from 4373 urine samples. published procedures were compared with 16s rrna sequencing and biochemical profiling (bbl-crystal-gp). 16s rrna sequencing and bbl-crystal-gp identified the same 13 isolates as a. urinae. published tests failed to distinguish the 13 a. urinae isolates from eight non-a. urinae isolates; several tests exhibited no discrimination. ciproflox ...200314616691
classification of homarus americanus hemocytes and the use of differential hemocyte counts in lobsters infected with aerococcus viridans var. homari (gaffkemia).hemocytes of the american lobster (homarus americanus h. milne edwards) were classified after examination of wright-giemsa stained cytocentrifuge preparations by brightfield light microscopy. eleven hemocyte types were identified using morphologic criteria. the classification system was then used to monitor changes in the differential hemocyte count (dhc) of lobsters infected with the gram positive coccus aerococcus viridans var. homari, etiologic agent of gaffkemia. the appearance of less matur ...200314726241
urinary tract infection caused by aerococcus viridans, a case report.aerococcus viridans organisms are gram-positive, usually airborne cocci that are widely distributed in hospital environments. these bacteria have infrequently been encountered as a human pathogen causing bacteremia, endocarditis and urinary tract infections. the clinical significance of these bacteria may be overlooked due to their fastidious growth and often confused with other strains of streptococci.200415507858
mastitis-causing streptococci are important contributors to bacterial counts in raw bulk tank milk.the objective of this study was to probe the contribution of streptococci to the microbial quality of raw milk. over a 5-month period, bulk tank milk samples from 48 new york state dairy farms were analyzed qualitatively for bacterial ecology and quantitatively for total bacterial, streptococcal, staphylococcal, and gram-negative bacterial counts. linear regression analysis was used to determine the contribution of differential counts to total bacterial counts. streptococci, staphylococci, and g ...200415633665
induced resistance to infection of lobsters homarus americanus by aerococcus viridans (var.) homari, the bacterium causing gaffkemia.a vaccine composed of steam sterilized (autoclaved) cells of a virulent strain of aerococcus viridans (var.) homari was effective in protecting lobsters homarus americanus against gaffkemia. at 15 degrees c the heat-killed vaccines (hkv) at concentrations between 1 and 5 x 10(7) particles kg(-1) lobster body wt induced maximal protection in induction periods ranging from 7 to 11 d. protection was substantial over the course of a 30 d post-induction trial period. spring-caught lobsters (i.e. thos ...200415672875
pcr detection of bacillus and staphylococcus in various foods.a broad-range pcr assay for the detection of bacteria belonging to bacillus and staphylococcus genera was developed. primers targeting the bacterial 16s rrna gene were newly designed and used in a pcr assay. to determine the specificity of the assay, 81 different bacterial strains (of 50 genera), 2 fungi, 3 animals, and 4 plants were tested. results were positive for every tested bacillus, staphylococcus, or aerococcus strain. in addition, the result for listeria grayi was positive with lower pc ...200415222564
studies on the virulence of aerococcus viridans (var.) homari, the causative agent of gaffkemia, a fatal disease of homarid lobsters.virulent and avirulent strains of aerococcus viridans (var.) homari were used to extend previous studies to determine and confirm differences between the 2 types. virulent strains possessed polysaccharide capsules and were not agglutinated by lobster hemolymph serum; avirulent strains did not have capsules, were agglutinated by the lobster hemolymph serum, and most did not grow well in lobster hemolymph serum. growth of the avirulent strains in sterile lobster hemolymph serum induced the product ...200415460859
septicaemia associated with an aerococcus viridans infection in immunodeficient mice.this report describes a case series of septicaemia caused by infection with aerococcus viridans in immunodeficient nod/ltsz-prkdc(scid) (nod/scid) mice. during a period of 3 weeks more than 40 animals died or became ill with clinical signs of ruffled coat, weight loss, laboured breeding, and distended abdomen. at necropsy it was found that the animals displayed symptoms of sepsis with widespread abscesses in the liver, heart, lungs or pyogenic peritonitis. a gram-positive coccus was isolated in ...200415207044
l-ficolin is a pattern recognition molecule specific for acetyl groups.l-ficolin and h-ficolin are molecules of the innate immune system. upon recognition of a suitable target they activate the complement system. the ligand recognition structure of ficolins is contained within a fibrinogen-like domain. we examined the selectivity of the ficolins through inhibiting the binding to bacteria or to beads coupled with n-acetylglucosamine. the binding of l-ficolin to streptococcus pneumoniae 11f and the beads was inhibited by n-acetylated sugars and not by non-acetylated ...200415331601
in vitro antimicrobial activity of gatifloxacin compared with other quinolones against clinical isolates from cancer patients.owing to the predominance of gram-positive pathogens in neutropenic cancer patients, newer generation quinolones with an expanded gram-positive spectrum and enhanced potency, may have a role to play for prophylaxis and/or empiric therapy in such patients. the in vitro activity of gatifloxacin was compared with that of ciprofloxacin, levofloxacin and trovafloxacin against 848 recent clinical isolates from cancer patients. against gram-positive organisms, gatifloxacin was the most active agent tes ...200415523180
response of american lobsters homarus americanus to infection with a field isolate of aerococcus viridans var. homari (gaffkemia): survival and haematology.american lobsters homarus americanus were inoculated with a field isolate of the gram-positive bacterium aerococcus viridans var. homari, causative agent of gaffkemia, at 1 x 10(6), 1 x 10(4) or 1 x 10(2) colony forming units (cfu) kg(-1) or with sterile 3% nacl and maintained at 10 or 15 degrees c until they died or were euthanised. progression of disease in individual animals was monitored daily by total haemocyte count (thc) and haemolymph culture. post-mortem examinations were performed on a ...200415609881
an unusual bacterium, aerococcus viridans, and four cases of infective endocarditis.four cases are described of aerococcus viridans endocarditis with favorable outcome, focusing on the clinical picture. extracardiac findings (e.g. neurological, renal, musculoskeletal, intra-abdominal) in infective endocarditis (ie) have been recognized more frequently in recent years, and may delay the diagnosis and have a negative influence on the prognosis. aerococcus viridans, a very rare microorganism causing invasive infections, has been associated with bacteremia, septic arthritis, and es ...200515974524
crystallization and preliminary x-ray diffraction study of l-lactate oxidase (lox), r181m mutant, from aerococcus viridans.l-lactate oxidase (lox) from aerococcus viridans is a member of the alpha-hydroxyacid oxidase flavoenzyme family. an x-ray crystallographic study of a lox mutant in which arg181 is replaced by met was initiated in order to understand the functions of the conserved amino-acid residues around the fmn in the enzyme active site. lox-r181m crystals belong to the tetragonal space group i422, with unit-cell parameters a = b = 192.632, c = 200.263 a, alpha = beta = gamma = 90 degrees. there are four mon ...200516511063
reclassification of pediococcus urinaeequi (ex mees 1934) garvie 1988 as aerococcus urinaeequi comb. nov.the taxonomic status of pediococcus urinaeequi is described, and the transfer of the species to the genus aerococcus with the name aerococcus urinaeequi comb. nov. is proposed, on the basis of the analysis of 16s rrna gene sequence and dna-dna hybridization data.200515879276
[gram-positive cocci as an opportunistic infection factor].the frequency of opportunistic infections increased in the last years. the opportunistic infections are serious and very difficult to diagnose complication at patients with lower immunity. the following factors are conducive to develop infections caused by opportunistic bacteria: invasive research methods, medical treatments and immunological defects. the publication presents pathogenicity of bacteria of following types: aerococcus, kocuria, kytococcus, lactococcus, leuconostoc, micrococcus, ped ...200516161935
antibacterial and antifungal lysozyme-type activity in cameraria ohridella pupae.lysozyme-type antibacterial and antifungal activity in pupae of cameraria ohridella was studied. activity against micrococcus luteus and bacillus megaterium was detected in pupae extract. also antifungal activity from c. ohridella pupae extract directed against saccharomyces cerevisiae strain w 303 was shown. during immunoblotting two bands in pupae extract, with molecular mass of about 15 and 28 kda were recognized by antibodies directed against hewl. after acid electrophoresis followed by bioa ...200516169556
identification of streptococcus spp. causing bovine mastitis by pcr-rflp of 16s-23s ribosomal dna.traditional microbiological methods for identification of streptococcus spp. causing bovine mastitis have been demonstrated to be less than highly reliable. pcr-rflp analysis of 16s-23s ribosomal dna was used to characterise seven reference strains of streptococcal mastitis pathogens as well as four reference strains of other gram-positive, catalase-negative cocci of bovine origin to allow comparative identification of field isolates. rflp analysis of pcr products, using a combination of two res ...200516289400
aerococcus urinae: polyphasic characterization of the species.a polyphasic characterization of aerococcus urinae is presented. in this study the intraspecies relationships between 26 strains of varying geographical origin were examined by phenotypic tests, ribotyping and multilocus enzyme electrophoresis. the results demonstrated two main phenotypic patterns that could be distinguished in tests for hydrolysis of aesculin, and acid production from amygdalin and salicin. strains were either negative (n=19) or positive (n=6) in these tests. one strain had a d ...200516086822
a case of aerococcus urinae septicemia: an unusual presentation and severe disease course. 200516117890
lytic enzyme, labiase for a broad range of gram-positive bacteria and its application to analyze functional dna/rna.the lytic activity of labiase and achromopeptidase for bacterial dna/rna extraction were compared. rapid lysis of many bacterial strains was observed with labiase followed by sds treatment. both labiase and achromopeptidase showed high lytic activity against bacterial strains with the a1alpha chemotype (e.g., aerococcus viridans) and the a3alpha chemotype (e.g., staphylococcus epidermidis) for cell wall peptidoglycan structures. the lytic activity of labiase was higher than that of achromopeptid ...200515722152
molecular cloning and characterization of cdna of penaeidin-like antimicrobial peptide from tiger shrimp (penaeus monodon).we report the molecular cloning and characterization of a penaeidin-like antimicrobial peptide (amp) complementary dna from the hemocytes of tiger shrimp (penaeus monodoni). a tiger shrimp amp cdna containing the entire coding region of the peptide, determined by rapid amplification of cdna 5' and 3' ends and polymerase chain reaction of the messenger rna isolated from hemocytes of the shrimp, is 683 bp in length with an open reading frame of 222 bp. the deduced amino acid sequence of this antim ...200515776314
genetic characterization of the lobster pathogen aerococcus viridans var. homari by 16s rrna gene sequence and rapd.a combination of 16s rrna sequencing and random amplified polymorphic dna (rapd) analysis was used to evaluate the genetic diversity within aerococcus viridans var. homari, the causative agent of gaffkemia in lobsters. a collection of 7 a. viridans var. homari strains and 2 avirulent a. viridans-like cocci isolated from homarid lobsters harvested from different regions on the atlantic coast of north america were analyzed. the isolates are separated geographically and temporally between the years ...200515819439
clinical significance of aerococcus urinae: a retrospective review.a retrospective chart review was performed on 54 patients with positive urine cultures during a 1-year period to assess the clinical significance of aerococcus urinae. based on predetermined criteria, patients were classified into 2 groups: those with urinary tract infections (utis) and those who were considered colonized. the majority of the patients were > or =65 years old and were female. only 31% of patients with uti and 45% of colonized patients had a. urinae isolated in pure cultures. both ...200516269223
effect of capsulation of opportunistic pathogenic bacteria on binding of the pattern recognition molecules mannan-binding lectin, l-ficolin, and h-ficolin.mannan-binding lectin (mbl), l-ficolin, and h-ficolin are pattern recognition molecules of the innate immune system. we investigated their ability to bind to different serotypes and noncapsulated variants of two gram-positive bacterial species, streptococcus pneumoniae and staphylococcus aureus. mbl did not bind to capsulated s. aureus or capsulated s. pneumoniae but did bind to a noncapsulated s. aureus variant (wood). l-ficolin bound to some capsulated s. aureus serotypes (serotypes 1, 8, 9, 1 ...200515664949
volatile and biogenic amines, microbiological counts, and bacterial amino acid decarboxylase activity throughout the salt-ripening process of anchovies (engraulis encrasicholus).chemical and microbiological parameters were studied during the industrial production of salt-ripened anchovies (engraulis encrasicholus). gradual acidification and increases in the proteolysis index and in total volatile basic nitrogen were observed. at the end of the maturing process, the values reached ph 5.55 +/- 0.03, 21.33 +/- 5.82%, and 44.06 +/- 12.47 mg/ 100 g, respectively. in the three studied anchovy batches, the biogenic amines tyramine, histamine, putrescine, cadaverine, and agmati ...200521132979
m-ficolin, an innate immune defence molecule, binds patterns of acetyl groups and activates complement.ficolins play a role in the innate immune defence as pathogen-associated molecular pattern recognition molecules. three ficolins are found in humans: h-ficolin, l-ficolin and m-ficolin. l-ficolin and h-ficolin circulate in blood in complexes with mannan-binding lectin-associated serine proteases (masps) and are capable of activating the complement system. l-ficolin shows affinity for acetylated compounds and binds to various capsulated strains of bacteria. h-ficolin has been shown to bind aeroco ...200516305643
[use of immobilized cells of bacteria in the process of purification of waste water containing chlorates and chromates].some regularities of immobilization of chlorate-reducing bacteria by various carriers and especially sewage treatment for chlorate and chromates by adhered bacteria have been studied. most bacterial cells are immobilized during the first hour of contact with the carrier. the studied carriers, as to their ability to adsorb aerococcus dechloraticans tgs-463 cells, may be arranged in the following sequence: porolon > claydite > glass broaches > maize stem > barley straw. the 12-24 hour culture aero ...200616686220
immunogen-dependent quantitative and qualitative differences in phagocytic responses of the circulating hemocytes of the lobster homarus americanus.phagocytic responses in circulating hemocytes of the lobster homarus americanus were measured before and after treatment of lobsters with 2 different immunogens: (1) lipolysaccharide (lps) or endotoxin from a non-pathogenic pseudomonas perolens, and (2) a vancomycin/live gram-positive pathogen (aerococcus viridans [var.] homari) combination, essentially attenuated cells, shown previously to induce a high degree of resistance to this pathogen. the responses elicited by each of the immunogens were ...200616724563
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