Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| role of purified β-1, 3 glucan binding protein (β-gbp) from paratelphusa hydrodromus and their anti-inflammatory, antioxidant and antibiofilm properties. | β- 1, 3-glucan binding protein (β-gbp), a pattern recognition protein (prp), plays a critical role in triggering the innate immune response by detecting β-glucan found on the surface of microbes. in the present study, β-gbp was purified from the haemolymph of rice field crab paratelphusa hydrodromus by affinity column chromatography. the monomeric protein ph-β-gbp appeared as a single band with a molecular weight of approximately 95 kda in sds-page analysis and its purity was determined to be 89 ... | 2017 | 28684323 |
| incidence, clinical characteristics, and outcomes of nosocomial enterococcus spp. bloodstream infections in a tertiary-care hospital in beijing, china: a four-year retrospective study. | enterococcus spp. are the common cause of nosocomial bloodstream infections (bsis) with high morbidity and mortality. the purpose of this study was to characterize the incidence, clinical and microbiological features, and mortality of nosocomial enterococcal bsis at a large chinese tertiary-care hospital in beijing, china. | 2017 | 28680588 |
| the enterococcus faecalis virulence factor elra interacts with the human four-and-a-half lim domains protein 2. | the commensal bacterium enterococcus faecalis is a common cause of nosocomial infections worldwide. the increasing prevalence of multi-antibiotic resistant e. faecalis strains reinforces this public health concern. despite numerous studies highlighting several pathology-related genetic traits, the molecular mechanisms of e. faecalis virulence remain poorly understood. in this work, we studied 23 bacterial proteins that could be considered as virulence factors or involved in the enterococcus inte ... | 2017 | 28676674 |
| analysis of the incidence and risk factors of male urinary tract infection following urodynamic study. | the purpose of this study was to investigate the incidence and risk factors of male urinary tract infection (uti) after urodynamic study (uds). a total of 854 consecutive male patients, who underwent uds at peking union medical college hospital from january 2010 to march 2016, were recruited in this study. two to four weeks before the examination, urinalysis with bacterial culture was performed. patients with negative results were selected for uds. immediately before the examination, urinalysis ... | 2017 | 28577157 |
| bactericidal activity and synergy studies of peptide ap-cect7121 against multi-resistant bacteria isolated from human and animal soft tissue infections. | ap-cect7121 is an antimicrobial peptide, produced by enterococcus faecalis cect7121, with bactericidal activity against gram-positive bacteria. the aim of this study was to evaluate the bactericidal activity of ap-cect7121, alone and with gentamicin, against multi-resistant bacteria isolated from human and animals with soft tissue infections. during the period 2014-2015, bacterial strains producing human and animal soft tissue infections were studied. samples from patients attended at a general ... | 2017 | 28573492 |
| detection of the optra gene in a clinical st16 enterococcus faecalis isolate in denmark. | 2017 | 28572038 | |
| phytochemical characterisation and bioprospection for antibacterial and antioxidant activities of lippia alba brown ex britton & wilson (verbenaceae). | ethanol extract and fractions obtained from fresh and dry aerial parts of lippia alba were examined in order to determine their phytochemical composition, antioxidant capacity and antibacterial activities. the ethanol extracts and fractions exhibited an antioxidant effect by the dpph assay, especially samples of fresh plant. hplc analysis of the ethyl acetate fractions identified the presence of phenolic acids and flavonoids. the ethanol extract and fractions showed activity against reference an ... | 2017 | 28571495 |
| comparative evaluation of different modes of laser assisted endodontics in primary teeth: an in vitro study. | the presence of bacteria in root canals has been considered to be responsible for endodontic treatment failure, even in case of primary teeth. the use of lasers can be a valuable addition in removing bacterial load in areas where traditional methods may fail to succeed. methods like direct laser irradiation of canals, laser activated irrigation (lai) with sodium hypochlorite (naocl) and photodynamic therapy (pdt) seem to be a promising alternative for disinfection. | 2017 | 28571279 |
| effects of diode laser, gaseous ozone, and medical dressings on enterococcus faecalis biofilms in the root canal ex vivo. | the objective was to compare the antibacterial effects of adjunctive disinfection using diode laser and gaseous ozone compared to the medical dressings calcium hydroxide (ca(oh)2) and chlorhexidine gel (chx-gel) on enterococcus faecalis biofilms in human root canals ex vivo. root canals of 180 human extracted teeth were infected by e. faecalis and divided into 3 main groups (g): g1, control; g2, instrumentation and irrigation using 0.9% nacl; g3, instrumentation and irrigation using 1% naocl. in ... | 2017 | 28567421 |
| virulence factors associated with enterococcus faecalis infective endocarditis: a mini review. | the enterococci are accountable for up to 20% of all cases of infective endocarditis, with enterococcus faecalis being the primary causative isolate. infective endocarditis is a life-threatening infection of the endocardium that results in the formation of vegetations. based on a literature review, this paper provides an overview of the virulence factors associated with e. faecalis infective endocarditis. furthermore, it reports the effects of active or passive immunization against some of these ... | 2017 | 28567146 |
| human bile reduces antimicrobial activity of selected antibiotics against enterococcus faecalis and escherichia coli in vitro. | it has been known from previous studies that body fluids, such as cerebrospinal fluid, lung surfactant, and urine, have a strong impact on the bacterial killing of many anti-infective agents. however, the influence of human bile on the antimicrobial activity of antibiotics is widely unknown. human bile was obtained and pooled from 11 patients undergoing cholecystectomy. after sterilization of the bile fluid by gamma irradiation, its effect on bacterial killing was investigated for linezolid (lzd ... | 2017 | 28559254 |
| collaborative research on puerperal infections in bangladesh. | bangladesh is considered as a high-risk country for emerging infectious diseases because of its high population density, poverty, and unhygienic conditions. although control efforts have primarily been focused on major infectious diseases such as diarrheal diseases, tuberculosis, malaria, and hiv infection, the prevalence and impact of many local or minor infectious diseases are still unclarified in this country. in this review, we present our recent experience and outcomes of collaborative rese ... | 2017 | 28552890 |
| stochasticity in the enterococcal sex pheromone response revealed by quantitative analysis of transcription in single cells. | in enterococcus faecalis, sex pheromone-mediated transfer of antibiotic resistance plasmids can occur under unfavorable conditions, for example, when inducing pheromone concentrations are low and inhibiting pheromone concentrations are high. to better understand this paradox, we adapted fluorescence in situ hybridization chain reaction (hcr) methodology for simultaneous quantification of multiple e. faecalis transcripts at the single cell level. we present direct evidence for variability in the ... | 2017 | 28671948 |
| carriage of multidrug resistant enterococcus faecium and enterococcus faecalis among apparently healthy humans. | enterococci are indigenous flora of the gastro-intestinal tracts of animals and humans. recently, interest in two major species, e. faecium and e. faecalis, has heightened because of their ability to cause serious infections and their intrinsic resistance to antimicrobials. this study was aimed at determining the prevalence of e. faecium and e. faecalis in human faecal samples and evaluating the susceptibility of the isolates to antibiotics. | 2017 | 28670644 |
| prevalence and genetic diversity of enterococcus faecalis isolates from mineral water and spring water in china. | enterococcus faecalis is an important opportunistic pathogen which is frequently detected in mineral water and spring water for human consumption and causes human urinary tract infections, endocarditis and neonatal sepsis. the aim of this study was to determine the prevalence, virulence genes, antimicrobial resistance and genetic diversity of e. faecalis from mineral water and spring water in china. of 314 water samples collected from january 2013 to january 2014, 48 samples (15.3%) were contami ... | 2017 | 28670302 |
| identification of enterococcus faecalis enzymes with azoreductases and/or nitroreductase activity. | nitroreductases, nad(p)h dependent flavoenzymes, are found in most of bacterial species. even if enterococcus faecalis strains seems to present such activity because of their sensitivity to nitrofurans, no enzyme has been described. nitroreductases were separated of others reductases due to their capacity to reduce nitro compounds. they are further classified based on their preference in cofactor: nadh and/or nadph. however, recently, azoreductases have been studied for their strong activity on ... | 2017 | 28545445 |
| influence of different wild-garlic (allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties. | as there are no previous studies of the european wild-garlic (allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts. | 2017 | 28543032 |
| antimicrobial resistance status of enterococcus from australian cattle populations at slaughter. | antimicrobial agents are used in cattle production systems for the prevention and control of bacterial associated diseases. a consequence of their use is the potential development of antimicrobial resistance (amr). enterococcus faecium and enterococcus faecalis that are resistant to antimicrobials are of increased concern to public health officials throughout the world as they may compromise the ability of various treatment regimens to control disease and infection in human medicine. australia i ... | 2017 | 28542602 |
| emergence of vana enterococcus faecium in denmark, 2005-15. | to describe the changing epidemiology of vancomycin-resistant enterococcus faecium and enterococcus faecalis in clinical samples in denmark 2005-15 according to species and van type, and, furthermore, to investigate the genetic relatedness of the clinical e. faecium isolates from 2015. | 2017 | 28541565 |
| the bacteriophage ef-p29 efficiently protects against lethal vancomycin-resistant enterococcus faecalis and alleviates gut microbiota imbalance in a murine bacteremia model. | enterococcus faecalis is becoming an increasingly important opportunistic pathogen worldwide, especially because it can cause life-threatening nosocomial infections. treating e. faecalis infections has become increasingly difficult because of the prevalence of multidrug-resistant e. faecalis strains. because bacteriophages show specificity for their bacterial hosts, there has been a growth in interest in using phage therapies to combat the rising incidence of multidrug-resistant bacterial infect ... | 2017 | 28536572 |
| effect of gnp functionalisation and multiple n-methylation of β-amyloid residue (32-37) on gram-positive bacterium. | in the previous report, the authors showed the gold nanoparticle (gnp) functionalised multiple n-methylated fragments of the residue (32-37) of beta (β)-amyloid protein (1-42), cggiglmvg and cgggggiglmvg toward disruption of β-amyloid (1-42), the predominant component of senile plaques. herein the in vitro antimicrobial activities of both normal and multiple n-methylated sequences of cggiglmvg and cgggggiglmvg were screened and it was found that all the eight sequences including four (non-functi ... | 2017 | 28530185 |
| occurrence of enterococcus faecalis and enterococcus faecium in various clinical infections: detection of their drug resistance and virulence determinants. | the aim of this study was to characterize virulence determinants and antibiotic resistance profiles in enterococci obtained from various clinical sources in the northwest of iran. a total of 160 enterococcal clinical isolates from various wards of university teaching hospitals were collected and specified by biochemical test, from september 2014 to july 2015. identification of enterococci was confirmed by multiplex pcr in the genus and species level. antibiotic resistance properties and virulenc ... | 2017 | 28525287 |
| mechanisms of peptide sex pheromone regulation of conjugation in enterococcus faecalis. | in many gram positive bacteria, horizontal transfer and virulence are regulated by peptide-mediated cell-cell signaling. the heptapeptide ccf10 (c) activates conjugative transfer of the enterococcus faecalis plasmid pcf10, whereas the icf10 (i) peptide inhibits transfer. both peptides bind to the same domain of the master transcription regulator prgx, a repressor of transcription of the prgq operon encoding conjugation genes. we show that repression of prgq by prgx tetramers requires formation o ... | 2017 | 28523739 |
| water and air ozone treatment as an alternative sanitizing technology. | we investigated the effectiveness of ozone (aqueous and gaseous) treatment as an alternative sanitizing technology to common conventional disinfectants in reducing the microbial contamination of both water and air. | 2017 | 28515631 |
| comparison of simplistic biofilm models for evaluating irrigating solutions. | many innovations have recently been implemented in the field of endodontics, often based on a variety of in-vitro/ex-vivo test setups. it was the goal of this research to compare different biofilm models for evaluating the effectiveness of rinsing solutions. | 2017 | 28512652 |
| comparative antimicrobial efficacy of eucalyptus galbie and myrtus communis l. extracts, chlorhexidine and sodium hypochlorite against enterococcus faecalis. | the aim of this study was to evaluate the antimicrobial effect of eucalyptusgalbie and myrtus communis l. methanolic extracts, chlorhexidine (chx) and sodium hypochlorite (naocl) on enterococcus faecalis (e. faecalis) as the predominant species isolated from infected root canals. | 2017 | 28512487 |
| comparative evaluation of three herbal solutions on the disinfection of guttapercha cones: an in vitro study. | guttapercha (gp) cones are usually supplied in aseptic, sealed packages from the manufacturers, but once exposed to the dental office environment or even by handling; they can be contaminated by a number of microorganisms. supplementary decontamination of gp cones is critical. as they cannot be sterilized by moist or dry heat, cold sterilization, using disinfectants should be used. | 2017 | 28511502 |
| rapid identification of vancomycin resistant enterococcus faecalis clinical isolates using a sugar fermentation method. | vancomycin resistant enterococci (vre) can be found all over the world. thus, rapid detection of the isolates could be of high importance in the treatment or prevention of the associated disease. | 2017 | 28511382 |
| development of a panel of recombinase polymerase amplification assays for detection of common bacterial urinary tract infection pathogens. | to develop and evaluate the performance of a panel of isothermal real-time recombinase polymerase amplification (rpa) assays for detection of common bacterial urinary tract infection (uti) pathogens. | 2017 | 28510991 |
| antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in saudi arabia. | premna resinosa (hochst.) schauer (lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage. | 2017 | 28508699 |
| the effect of sodium hypochlorite concentration and irrigation needle extension on biofilm removal from a simulated root canal model. | to investigate the effect of sodium hypochlorite concentration and needle extension on removal of enterococcus faecalis biofilm, sixty root canal models were 3d printed. biofilms were grown on the apical 3 mm of the canal for 10 days. irrigation for 60s with 9 ml of either 5.25% or 2.5% naocl or water was performed using a needle inserted either 3 or 2 mm from the canal terminus and imaged using fluorescence microscopy and residual biofilm percentages were calculated using imaging software. the ... | 2017 | 28508523 |
| empyema thoracis caused by an optra-positive and linezolid-intermediate enterococcus faecalis strain. | 2017 | 28506787 | |
| chemical composition, antibacterial, schistosomicidal, and cytotoxic activities of the essential oil of dysphania ambrosioides (l.) mosyakin & clemants (chenopodiaceae). | we have investigated the chemical composition and the antibacterial activity of the essential oil of dysphania ambrosioides (l.) mosyakin & clemants (chenopodiaceae) (da-eo) against a representative panel of cariogenic bacteria. we have also assessed the in vitro schistosomicidal effects of da-eo on schistosoma mansoni and its cytotoxicity to gm07492-a cells in vitro. gas chromatography (gc) and gas chromatography-mass spectrometry (gc/ms) revealed that the monoterpenes cis-piperitone oxide (35. ... | 2017 | 28504841 |
| metabolic impact of nutrient starvation in mevalonate-producing escherichia coli. | the aim of this work was to enhance mevalonate yield from glucose in escherichia coli by essential nutrient starvations and to reveal these effects on the central carbon metabolism. stationary phase culture without essential nutrients such as nitrogen, sulfur, and magnesium was evaluated using an engineered e. coli introducing mvae and mvas genes from enterococcus faecalis. sulfur starvation resulted in the highest mevalonate yield of 0.61c-molc-mol(-1) from glucose. the metabolic impacts of nut ... | 2017 | 28501379 |
| occurrence of ampicillin-resistant enterococcus faecium carrying esp gene in pet animals: an upcoming threat for pet lovers. | this study was carried out to investigate oral colonisation by enterococcus faecalis and enterococcus faecium in pet dogs and cats, with special reference to antibiotic resistance. | 2017 | 28499907 |
| an iterative glycosyltransferase ents catalyzes transfer and extension of o- and s-linked monosaccharide in enterocin 96. | glycosyltransferases are essential tools for in vitro-glycoengineering. bacteria harbor an unexplored variety of protein glycosyltransferases. here, we describe a peptide glycosyltransferase (ents) encoded by orf0417 of enterococcus faecalis tx0104. ents di-glycosylates linear peptide of enterocin 96- a known antibacterial, in vitro. it is capable of transferring as well as extending the glycan onto the peptide in an iterative sequential dissociative manner. it can catalyze multiple linkages: gl ... | 2017 | 28498962 |
| etiological and resistance profile of bacteria involved in urinary tract infections in young children. | background. the objective of this study was to identify the bacteria most frequently responsible for urinary tract infection (uti) in the population of under-2-year-olds in our geographic area and to evaluate the activity of antibiotics widely used for uti treatment during a 4-year study period. materials and methods. a retrospective analysis was conducted of data on the identification and susceptibility of microorganisms isolated in urine samples from children under 2 years of age. results. a t ... | 2017 | 28497052 |
| antimicrobial resistance patterns in women with positive urine culture: does menopausal status make a significant difference? | aim. urinary tract infection (uti) is considered one of the most common bacterial infections in women. the aim of this study was to investigate the types of uropathogens present, as well as the degree of antimicrobial drug resistance seen among premenopausal (n = 2748) and postmenopausal (n = 1705) women with uncomplicated uti. methods. urinary samples (n = 4453) collected from women with uti were analyzed in terms of uropathogens present. these were considered as positive if bacterial growth wa ... | 2017 | 28497048 |
| evaluation of the synergistic effect of tomatidine with several antibiotics against standard and clinical isolates of staphylococcus aureus, enterococcus faecalis, pseudomonas aeruginosa and escherichia coli. | antibiotic resistance is an important problem in antibiotic treatment of infections, particularly in hospitals. tomatidine is a plant secondary metabolite with antimicrobial and antifungal effects. this study examined the possible synergistic effect tomatidine with several antibiotics against standard and clinical strains of staphylococcus aureus, enterococcus faecalis, pseudomonas aeruginosa and escherichia coli. after determining the minimum inhibitory concentrations (mics) of antibiotics and ... | 2017 | 28496482 |
| chemical composition, antibacterial and antifungal activities of flowerhead and root essential oils of santolina chamaecyparissus l., growing wild in tunisia. | the antimicrobial properties of essential oil from various santolina species have not been investigated enough in the previous studies dealing with the biological activities of medicinal plants. in tunisia, santolina chamaecyparissus l. (asteraceae) is the only santolina species recorded and is used as vermifuge and emmenagogue. the chemical composition, antibacterial and antifungal properties of essential oils from the flowerheads and roots of spontaneous s. chamaecyparissus growing in tunisia ... | 2017 | 28490960 |
| the most frequent isolates from outpatients with urinary tract infection. | the main objective of the study was to identify the most frequent isolates from female outpatients with urinary tract infection. | 2017 | 28484348 |
| determining the optimal dose of 1940-nm thulium fiber laser for assisting the endodontic treatment. | insufficient cleaning, the complex anatomy of the root canal system, inaccessible accessory canals, and inadequate penetration of irrigants through dentinal tubules minimizes the success of the conventional endodontic treatment. laser-assisted endodontic treatment enhances the quality of conventional treatment, but each laser wavelength has its own its own limitations. the optimal parameters for the antibacterial efficiency of a new wavelength, 1940-nm thulium fiber laser, were firstly investiga ... | 2017 | 28669067 |
| antibacterial effect of genetically-engineered bacteriophage ϕef11/ϕfl1c(δ36)p(nisa) on dentin infected with antibiotic-resistant enterococcus faecalis. | enterococcus faecalis is a gram-positive facultative anaerobic bacterium, which is present in 30-89% of teeth with postendodontic treatment failures. e. faecalis is capable of penetrating dentinal tubules and surviving as a monoculture after conventional endodontic therapy, indicating that it is resistant to commonly used endodontic disinfection protocols. different e. faecalis strains have shown resistance to several antibiotics, and have been associated with both dental pathology and systemic ... | 2017 | 28646693 |
| cytoprotective effect of the enzyme-mediated polygallic acid on fibroblast cells under exposure of uv-irradiation. | the poly(gallic acid), produced by laccase-mediated oxidation of gallic acid in aqueous media (ph5.5) to attain a novel material with well-defined molecular structure and high water solubility (500mg/ml at 25°c), has been investigated to understand its potential biological activities. in this regard, a biomedical approach based on cytoprotective effect on human fibroblast cells exposed to uv-irradiation in the presence of the polymer has been demonstrated. the results also shows that 200μg/ml of ... | 2017 | 28482545 |
| use of photo-fenton's reaction by 400-nm led light for endodontic disinfection: a preliminary in vitro study on enterococcus faecalis. | one of the biggest challenges in endodontics is the complete disinfection of root canals. in addition to mechanical preparation, the technique traditionally also involves channel disinfection with other agents such as sodium hypochlorite, hydrogen peroxide, chlorhexidine, or a combination of these. some bacterial species are particularly resistant to eradication. using enterococcus faecalis in this preliminary study, we tested the bactericidal effectiveness of the fenton reaction and the photo-f ... | 2017 | 28482224 |
| daptomycin: new insights into an antibiotic of last resort. | 2017 | 28481142 | |
| studies on chemical composition and antimicrobial activities of bioactive molecules from date palm (phoenixdactylifera l.) pollens and seeds. | natural resources have been the crucial origin of chemical elements. they have been used in many traditions as alternative medicines. the chemical profiling of some plant extracts and essential oils related to different plants were followed to unveil their most active components. in this paper, phoenix dactilyfera l was selected as a host plant to investigate the composition of different organs with different cultivars. | 2017 | 28480436 |
| does the removal of cementum facilitate bacterial penetration into dentinal tubules in vitro? | the importance of an intact layer of cementum on the root surface in preventing bacterial penetration into radicular dentin has not been sufficiently investigated. the aim of this in vitro study was to determine the effect of the absence of cementum from the root surface and the length of the infection period (2 or 4 weeks) on the maximum depth of bacterial penetration and the percentage of sectors lined with bacteria. | 2017 | 28476468 |
| urinary tract infections in postmenopausal women with type 2 diabetes: clinical correlates and quinolone susceptibility. | introduction diabetes mellitus and the postmenopausal period are associated with increased risk of urinary tract infections (utis) in women. however, data on utis in postmenopausal diabetic women are scarce. objectives the aim of this study was to determine the prevalence of utis in postmenopausal women with type 2 diabetes mellitus, identify the potential risk factors, describe the causative pathogens, and assess their susceptibility to quinolones. patients and methods patients were interviewe ... | 2017 | 28475170 |
| a short artificial antimicrobial peptide shows potential to prevent or treat bone infections. | infection of bone is a severe complication due to the variety of bacteria causing it, their resistance against classical antibiotics, the formation of a biofilm and the difficulty to eradicate it. antimicrobial peptides (amps) are naturally occurring peptides and promising candidates for treatment of joint infections. this study aimed to analyze the effect of short artificial peptides derived from an optimized library regarding (1) antimicrobial effect on different bacterial species, (2) efficac ... | 2017 | 28473710 |
| phtβ-promoted mobilization of non-conjugative resistance plasmids from enterococcus faecium to enterococcus faecalis. | to analyse the recombination events associated with conjugal mobilization of two multiresistance plasmids, prum17i48 and plag (formerly named pdo1-like), from enterococcus faecium 17i48 to enterococcus faecalis jh2-2. | 2017 | 28645197 |
| methacrylate-based root canal sealer containing chlorexidine and α-tricalcium phosphate. | the aim of this study was to develop and to characterize a methacrylate-based root canal sealer containing chlorhexidine (chx) and α-tricalcium phosphate (α-tcp). experimental dual cure methacrylate-based sealer was produced containing 0, 2.5, or 5 wt% of chx and 0, 25, or 50 wt% of α-tcp. experimental sealers were evaluated based on flow, film thickness, radiopacity, degree of conversion (dc), degradation in water, ph and antibacterial activity. flow ranged from 15.09 ± 0.11 to 17.47 ± 0.42 mm. ... | 2017 | 28644564 |
| role of surface energy and nano-roughness in the removal efficiency of bacterial contamination by nonwoven wipes from frequently touched surfaces. | healthcare associated infections (hcais) are responsible for substantial patient morbidity, mortality and economic cost. infection control strategies for reducing rates of transmission include the use of nonwoven wipes to remove pathogenic bacteria from frequently touched surfaces. wiping is a dynamic process that involves physicochemical mechanisms to detach and transfer bacteria to fibre surfaces within the wipe. the purpose of this study was to determine the extent to which systematic changes ... | 2017 | 28469734 |
| molecular mechanism of quorum-sensing in enterococcus faecalis: its role in virulence and therapeutic approaches. | quorum-sensing systems control major virulence determinants in enterococcusfaecalis, which causes nosocomial infections. the e. faecalis quorum-sensing systems include several virulence factors that are regulated by the cytolysin operon, which encodes the cytolysin toxin. in addition, the e. faecalis fsr regulator system controls the expression of gelatinase, serine protease, and enterocin o16. the cytolysin and fsr virulence factor systems are linked to enterococcal diseases that affect the hea ... | 2017 | 28467378 |
| total staphylococci as performance surrogate for greywater treatment. | faecal indicator bacteria (fib) are commonly used as water quality indicators; implying faecal contamination and therefore the potential presence of pathogenic enteric bacteria, viruses, and protozoa. hence in wastewater treatment, the most commonly used treatment process measures (surrogates) are total coliforms, faecal coliforms, escherichia coli (e. coli), and enterococci. however, greywater potentially contains skin pathogens unrelated to faecal load, and e. coli and other fib may grow withi ... | 2017 | 28462431 |
| applications of copolymer for rapid identification of bacteria in blood culture broths using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. | matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) can be used to identify pathogens in blood culture samples. however, sample pretreatment is needed for direct identification of microbes in blood culture bottles. conventional protocols are complex and time-consuming. therefore, in this study, we developed a method for collecting bacteria using polyallylamine-polystyrene copolymer for application in wastewater treatment technology. using representative ba ... | 2017 | 28461023 |
| bacterial diversity of the gastric content of preterm infants during their first month of life at the hospital. | studies focused on the stomach microbiota are relatively scarce, and most of them are focused on the adult population. the aim of this work is to describe the bacterial communities inhabiting the gastric content (gc) of preterm neonates. for that purpose, gc samples were collected weekly from a total of 13 preterm neonates during their first month of life within their hospital stay. samples were analyzed by using both culture-dependent and -independent techniques. the former allowed the isolatio ... | 2017 | 28459051 |
| transcriptomics analysis reveals putative genes involved in biofilm formation and biofilm-associated drug resistance of enterococcus faecalis. | enterococcus faecalis is a gram-positive bacterium associated with endodontic infections and is capable of forming biofilms that can confer drug resistance to the bacterium, resulting in treatment failure. current knowledge on e. faecalis drug resistance is of a limited and conflicting nature. the present study examined the genetic basis of e. faecalis biofilm formation and drug resistance using a rna sequencing (rna-seq)-based transcriptome approach. | 2017 | 28457636 |
| exploiting crispr-cas to manipulate enterococcus faecalis populations. | crispr-cas provides a barrier to horizontal gene transfer in prokaryotes. it was previously observed that functional crispr-cas systems are absent from multidrug-resistant (mdr) enterococcus faecalis, which only possess an orphan crispr locus, termed crispr2, lacking cas genes. here, we investigate how the interplay between crispr-cas genome defense and antibiotic selection for mobile genetic elements shapes in vitro e. faecalis populations. we demonstrate that crispr2 can be reactivated for gen ... | 2017 | 28644125 |
| synthesis and antimicrobial activity of chiral quaternary n-spiro ammonium bromides with 3',4'-dihydro-1'h-spiro[isoindoline-2,2'-isoquinoline] skeleton. | a new class of highly functionalized tetrahydroisoquinolines with a quaternary carbon stereocenter was synthesized starting from an easily accessible l-tartaric acid. nine strains of bacteria (staphylococcus aureus, streptococcus pyogenes, streptococcus mutans, streptococcus salivarius, bacillus subtilis, enterococcus faecalis, moraxella catarrhalis, escherichia coli, campylobacter jejuni) were used for the determination of minimal inhibitory concentration (mic) and minimal bactericidal concentr ... | 2017 | 28740363 |
| a prospective randomised trial of isolated pathogens of surgical site infections (ssi). | every surgical wound is colonized by bacteria, but only a small percentage displays symptoms of infection. the distribution of pathogens isolated in surgical site infections has not significantly changed over the last decades. staph. aureus, coag(-) staphylococci, enterococcus spp and e. coli are the main strains appearing. in addition, a continuously rising proportion of surgical site infections caused by resistant bacterial species (mrsa, c. albicans) has been reported. | 2017 | 28761643 |
| implementation of the new virtuo blood culture system: evaluation and comparison to the 3d system using simulated blood cultures. | to evaluate the performances of the newly approved bact/alert virtuo blood culture system for the recovery of bloodstream pathogens and compare it to the bact/alert 3d system. | 2017 | 28583022 |
| cyclohexene-fused 1,3-oxazines with selective antibacterial and antiparasitic action and low cytotoxic effects. | oxazine derivatives, a class of heterocyclic compounds, exhibit a variety of biological properties, such as anticonvulsant and antitumor activities. in this study, we evaluated the effect of two cyclohexene-fused 1,3-oxazines (cis‑1-benzyl-n-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (1) and trans‑n-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (2)) in cultures of bacillus cereus, enterococcus faecalis, escherichia coli, klebsiella pneumoniae, salmonella enterica, serratia mar ... | 2017 | 28755871 |
| detection of eight foodborne bacterial pathogens by oligonucleotide array hybridization. | simultaneous and rapid detection of multiple foodborne bacterial pathogens is important for the prevention of foodborne illnesses. | 2017 | 28713514 |
| high level bacterial contamination of secondary school students' mobile phones. | while contamination of mobile phones in the hospital has been found to be common in several studies, little information about bacterial abundance on phones used in the community is available. our aim was to quantitatively determine the bacterial contamination of secondary school students' mobile phones. | 2017 | 28626737 |
| self assembled snowball-like hybrid nanostructures comprising viburnum opulus l. extract and metal ions for antimicrobial and catalytic applications. | herein, we report the production of novel organic-inorganic nanobio-catalytic and antimicrobial agents called "nanosnowball" (nsbs) with a rational design and elucidate the increase in the catalytic and antimicrobial activities. the nsbs resemble to guelder rose were formed of the extract of viburnum opulus (vo) as the organic component and copper (ii) ions (cu(2+)) as the inorganic component. the effects of the vo extract and cu(2+) concentrations on the morphology of nsbs were systematically e ... | 2017 | 28465062 |
| use of whey as a culture medium for bacillus clausii for the production of protein hydrolysates with antimicrobial and antioxidant activity. | the use of whey as a natural culture medium to produce hydrolysates with antimicrobial from bacillus clausii is the objective of this research. the fermentation process was carried out at 25 ℃ for five days, where proteolytic activity, soluble peptides quantification, and antimicrobial activity using the disc diffusion method were determined every day. the fermented whey reached values of proteolytic activity between 90 and 260 u ml(-1) and a production of soluble peptides between 100 and 2070 ... | 2017 | 28816527 |
| growth inhibition and antibiofilm potential of ag nanoparticles coated with lectin, an arthropod immune molecule. | lectins from the haemolymph of arthropods, including crustaceans, are molecules potentially involved in the immune recognition and phagocytosis. here, lectin was purified from the haemolymph of blue swimmer crab portunus pelagicus, using mannose-coupled sepharose cl-4b affinity column chromatography. in sds-page analysis, lectin showed a molecular mass of approximately 155kda. the synthesis of lectin-coated silver nanoparticles (lectin-coated agnps) was confirmed by uv-vis spectroscopy, xrd, fti ... | 2017 | 28441606 |
| in vitro evaluation of cytotoxic, anti-proliferative, anti-oxidant, apoptotic, and anti-microbial activities of cladonia pocillum. | the aim of this study was to investigate the anti-proliferative, apoptotic, cytotoxic, and anti-oxidant effects of extracts from the lichen cladonia pocillumon human breast cancer cells (mcf-7), and to characterize the anti-microbial features. mcf-7 cells were treated with methanolic c. pocillum extract for 24h. the cytotoxicity of the extract was tested with mtt. moreover, its anti-proliferative effects were examined with immunocytochemical method. apoptosis and biochemical parameters were det ... | 2017 | 28838343 |
| investigation of effect of 1,8-cineole on antimicrobial activity of chlorhexidine gluconate. | chlorhexidine belongs to a group of medicines called antiseptic antibacterial agents. chlorhexidine is commonly used for the care and clean off the skin, hands, and wounds. in recent years, medicinal and aromatic plants have been used for prevention of disease, maintaining health, and improving disease in traditional and modern medicine as a medicament. according to recent research, cineole is the isolated active agent of eucalyptus oil and possesses antimicrobial activity. it was demonstrated t ... | 2017 | 28827963 |
| proinflammatory mg-63 cells response infection with enterococcus faecalis cps2 evaluated by the expression of tlr-2, il-1β, and inos mrna. | we have previously demonstrated that unencapsulated enterococcus faecalis cps2 inhibits biofilm formation of candida albicans, a fungus commonly found with e. faecalis in periapical lesion. in this study, we compared encapsulated and unencapsulated e. faecalis cps2 strains relationship with osteoblastic (mg-63) cells, whereas e. faecalis atcc 29212 were used as a reference strain. | 2017 | 28800779 |
| azathioprine, mercaptopurine, and 5-aminosalicylic acid affect the growth of ibd-associated campylobacter species and other enteric microbes. | campylobacter concisus is a bacterium that is associated with inflammatory bowel disease (ibd). immunosuppressive drugs including azathioprine (aza) and mercaptopurine (mp), and anti-inflammatory drug such as 5-aminosalicylic acid (5-asa) are commonly used to treat patients with ibd. this study aimed to examine the effects of aza, mp, and 5-asa on the growth of ibd-associated bacterial species and to identify bacterial enzymes involved in immunosuppressive drug metabolism. a total of 15 bacteria ... | 2017 | 28424670 |
| thymus vulgaris l. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity. | this study evaluated the biological effects of the t. vulgaris l. extract., such as antimicrobial activity on planktonic cultures and mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory activity and genotoxicity. | 2017 | 28683409 |
| safety evaluation of enterocin producer enterococcus sp. strains isolated from traditional turkish cheeses. | the purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in enterococcus spp. isolated from different cheeses and also investigate some of their virulence factors. enterococcus strains were isolated from 33 different cheeses. enterococcus faecium (6 strains) and enterococcus faecalis (5 strains) enterocin-producing strains were identified by 16s rdna analyses. structural genes enta, entb, entp and entx were detected in some isolates. mu ... | 2017 | 28735309 |
| isolation and characterization of lactic acid bacteria from traditional pickles of himachal pradesh, india. | a total of 15 isolates of lactic acid bacteria (lab) were isolated from traditional pickles of rural and urban areas of himachal pradesh. these isolates were identified as enterococcus faecalis (7), lactobacillus plantarum (3), pediococcus pentosaceus (2), leuconostoc mesenteroides (1), lactococcus lactis (1) and enterococcus sp. (1) on the basis of morphological, biochemical and 16s rdna sequence analysis. all the labs produced exopolysaccharides, have antibiotic susceptibility, and possess act ... | 2017 | 28720951 |
| inactivation of bacterial biofilms by visible light-activated unmodified zno nanorods. | various zinc oxide (zno) nanostructures are widely used for photocatalytic antibacterial applications. since zno possess a wide bandgap, it is believed that only uv light may efficiently assist bacterial inactivation and diverse crystal lattice modifications should be applied in order to narrow the bandgap for efficient visible light absorption. in this work we show that even unmodified zno nanorods grown by aqueous chemical growth technique were found to possess intrinsic defects which can be a ... | 2017 | 28628486 |
| characterization of high exopolysaccharide-producing lactobacillus strains isolated from mustard pickles for potential probiotic applications. | the aim of this study was to characterize high exopolysaccharide (eps)-producing lactic acid bacteria (lab) isolated from mustard pickles in taiwan for potential probiotic applications. among 39 collected lab strains, four most productive eps-producing strains were selected for further analysis. comparative analyses of 16s rdna genes rpoa and phes sequences demonstrated that these strains were members of lactobacillus plantarum-group (lpg). ncd 2, nld 4, slc 13, and nld 16 showed survival rates ... | 2017 | 28617525 |
| seven new lignan glycosides from the branches of alangium kurzii craib var. laxifolium. | seven new lignan glycosides (1-3, 8-10, and 14) and 17 known compounds were isolated from the branches of alangium kurzii craib var. laxifolium. their structures were established by spectroscopic analysis and circular dichroism (cd) and x-ray analysis. the isolated compounds were evaluated for their antibacterial activities against staphylococcus aureus ci1011, streptococcus suis ci1608, salmonella gallinarum ci0912, enterococcus faecalis ci1304, aeromonas hydrophila ci1008, escherichia coli ci1 ... | 2017 | 28733150 |
| the antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human hel 12469 cells. | six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. antimicrobial activity was shown against pathogenic (escherichia coli, salmonella typhimurium, yersinia enterocolitica, staphylococcus aureus, listeria monocytogenes, and enterococcus faecalis) and environmental bacteria (bacillus cereus, arthrobacter protophormiae, pseudomonas fragi) and fungi (chaetomium globosum, penicillium chrysogenum, cladospori ... | 2017 | 28811611 |
| phytochemical screening, antimicrobial and cytotoxicity studies of ethanol leaf extract of aphaniasenegalensis (sapindaceae). | aphania senegalensis (sapindaceae) is commonly used in senegalese traditional medicine to treat pain, inflammation, asthenia, bacterial and fungal infections. the aim of this study was to determine the type of phytochemical constituents present in the ethanol leaf extract and its antimicrobial activity against selected bacterial and fungal pathogens. | 2017 | 28638876 |
| [distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015: results from a multicenter, retrospective study]. | objective: to describe the distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015 to provide reference for empirical anti-infection treatment. methods: pathogens were from hematology department of 26 tertiary hospitals in jiangsu province from 2014 to 2015. antimicrobial susceptibility testing was carried out according to a unified protocol using kirby-bauer method or agar dilution method. collection of drug susceptibility results and corresp ... | 2017 | 28810329 |
| efficacy of radiant catalytic ionization to reduce bacterial populations in air and on different surfaces. | air contamination by biological agents is often observed in medical or veterinary facilities and industrial plants. bioaerosols may sediment and pose the surface contamination. microorganisms present on them may become a source of infections among humans and food contamination. this study determined the use of oxidative gases, including ozone and peroxide, generated by the radiant catalytic ionization (rci) cell for the inactivation of acinetobacter baumannii, escherichia coli, enterococcus faec ... | 2017 | 28803189 |
| antimicrobial activities of widely consumed herbal teas, alone or in combination with antibiotics: an in vitro study. | because of increasing antibiotic resistance, herbal teas are the most popular natural alternatives for the treatment of infectious diseases, and are currently gaining more importance. we examined the antimicrobial activities of 31 herbal teas both alone and in combination with antibiotics or antifungals against some standard and clinical isolates of pseudomonas aeruginosa, acinetobacter baumannii, escherichia coli, klebsiella pneumoniae, enterococcus faecalis, methicillin susceptible/resistant s ... | 2017 | 28761777 |
| antimicrobial susceptibility among gram-positive and gram-negative organisms collected from the latin american region between 2004 and 2015 as part of the tigecycline evaluation and surveillance trial. | the in vitro activity of tigecycline and comparator agents was evaluated against gram-positive and gram-negative isolates collected in latin american centers between 2004 and 2015 as part of the tigecycline evaluation and surveillance trial (t.e.s.t.) global surveillance study. | 2017 | 28701170 |
| investigation and analysis of the characteristics and drug sensitivity of bacteria in skin ulcer infections. | skin ulcer is a common type of disease affecting patients' health and quality of life, and bacterial infection increases the difficulty of its management. | 2017 | 28689800 |
| patterns of multi-drug resistant bacteria at first culture from patients admitted to a third level university hospital in calabria from 2011 to 2014: implications for empirical therapy and infection control. | surveillance of antimicrobial drug resistance is fundamental to guide empirical treatment. however, the european antimicrobial resistance surveillance network provides a general picture, which might not be applicable to clinical settings that are excluded from this survey. we evaluated resistance patterns of eskape isolates over a four-year period in a third level university hospital in the province of catanzaro (southern italy). in this retrospective study, we evaluated the frequency of eskape ... | 2017 | 28603227 |
| evaluation of the in vitro antimicrobial activity of selected saudi scorpion venoms tested against multidrug-resistant micro-organisms. | scorpion venoms are a rich source of bioactive peptides with promising clinical value that may lead to the discovery and development of new drugs. the present study was designed to evaluate the in vitro antimicrobial activities of the venoms extracted from three medically important saudi scorpions (androctonus crassicauda, androctonus bicolor and leiurus quinquestriatus). | 2017 | 28587870 |
| in vitro selection and identification of potential probiotics isolated from the gastrointestinal tract of nile tilapia, oreochromis niloticus. | fish gut bacteria can be used as probiotics for aquaculture. the aim of this study is to screen and identify beneficial probiotic bacteria from the gut of nile tilapia, oreochromis niloticus. nine out of one hundred thirty-five isolates were non-pathogenic through intraperitoneal injection and had antibacterial activities with at least a strain from the five isolated fish pathogens, aeromonas sobria, aeromonas hydrophila, pseudomonas aeruginosa, pseudomonas putida, and staphylococcus aureus. fur ... | 2017 | 28819794 |
| induction of cryptic and bioactive metabolites through natural dietary components in an endophytic fungus colletotrichum gloeosporioides (penz.) sacc. | grape skin and turmeric extracts having the major components resveratrol and curcumin, respectively, were used for the induction of cryptic and bioactive metabolites in an endophytic fungus colletotrichum gloeosporioides isolated from syzygium cumini. the increase in total amount of crude compounds in grape skin and turmeric extract treated cultures was 272.48 and 174.32%, respectively, compared to the untreated control. among six human pathogenic bacteria tested, the maximum inhibitory activity ... | 2017 | 28674526 |
| antimicrobial and antioxidant activities of the extract and fractions of tetradenia riparia (hochst.) codd (lamiaceae) leaves from brazil. | tetradenia riparia (lamiaceae) is native to central africa popularly known as myrrh, used in folk medicine to treat various diseases like malaria, gastroenteritis, and tropical skin disease. this research was to evaluate the antioxidant and antibacterial activities of the crude extract (ce) and fractions (fr) of the t. riparia by classical chromatography. the ce of t. riparia leaves was submitted to column chromatographic fractionation to obtain four fractions of the interest, which were identif ... | 2017 | 28840299 |
| comparative antimicrobial efficacy of selected root canal irrigants on commonly isolated microorganisms in endodontic infection. | this study aims to evaluate and compare the antimicrobial efficacy of three selected root canal irrigants (biopure mtad, metronidazole, aztreonam) against microbes commonly isolated from polymicrobial microbiota of root canal infection. | 2017 | 28435359 |
| efficacy and safety of probiotic-supplemented triple therapy for eradication of helicobacter pylori in children: a systematic review and network meta-analysis. | the aim of this study was to identify the best probiotic supplementation in triple therapy for pediatric population with helicobacter pylori infection. | 2017 | 28681177 |
| [bifidobacterium scardovii isolated from 2 consecutive urine samples]. | bifidobacterium scardovii species consists of facultative anaerobic gram-positive rods whose growth is stimulated by co2 and anaerobiosis. exceptionally it has been associated with infections in humans. an elderly male patient with a urinary tract infection due to b. scardovii and enterococcus faecalis is presented here; both microorganisms were isolated from two consecutive urine samples. the bacillus did not grow on standard media, but on chocolate agar incubated in co2 and on supplemented bru ... | 2017 | 28734711 |
| antibacterial and antifungal activity of endodontic intracanal medications. | the sterilization of the entire root canal system represents the main goal of every endodontist, given the fact that the control of the microbial flora is the key point of every root canal treatment. the diversity of microorganisms found inside the root canal and also the resistance of some bacterial species to intracanal medications led to a continuous development of new endodontic products. the present study focuses on the comparison of the antibacterial and antifungal properties of different ... | 2017 | 28781531 |
| genomic insights into the pathogenicity and environmental adaptability of enterococcus hirae r17 isolated from pork offered for retail sale. | genetic information about enterococcus hirae is limited, a feature that has compromised our understanding of these clinically challenging bacteria. in this study, comparative analysis was performed of e. hirae r17, a daptomycin-resistant strain isolated from pork purchased from a retail market in beijing, china, and three other enterococcal genomes (enterococcus faecium do, enterococcus faecalis v583, and e. hirae atcc(™) 9790). some 1,412 genes were identified that represented the core genome t ... | 2017 | 28799224 |
| methyl propiolate and 3-butynone: starting points for synthesis of amphiphilic 1,2,3-triazole peptidomimetics for antimicrobial evaluation. | a library of 29 small 1,4-substituted 1,2,3-triazoles was prepared for studies of antimicrobial activity. the pharmacophore model investigated with these substrates was based on small peptidomimetics of antimicrobial peptides and antimicrobials isolated from marine organisms from sub-arctic regions. using methyl 1,2,3-triazole-carboxylates and 1,2,3-triazole methyl ketones prepared through "click" chemistry we were able to synthesize the different cationic amphiphiles through three steps or less ... | 2017 | 28797773 |
| antimicrobial and immunomodulatory activity of host defense peptides, clavanins and ll-37, in vitro: an endodontic perspective. | endodontic treatment is mainly based on root canal disinfection and its failure may be motivated by microbial resistance. endodontic therapy can be benefitted by host defense peptides (hdps), which are multifunctional molecules that act against persistent infection and inflammation. this study aimed to evaluate the antimicrobial, cytotoxic and immunomodulatory activity of several hdps, namely clavanin a, clavanin a modified (mo) and ll-37, compared to intracanal medication ca(oh)2. hdps and ca(o ... | 2017 | 28712894 |
| comparison of the antimicrobial efficacy of calcium hydroxide and photodynamic therapy against enterococcus faecalis and candida albicans in teeth with periapical lesions; an in vivo study. | introduction: elimination of pathological microflora of root canal systems is a major goal in endodontic treatment. this study aimed to compare the antimicrobial efficacy of calcium hydroxide as an intracanal medication and antibacterial photodynamic therapy (apdt) against enterococcus faecalis and candida albicans in teeth with periapical (pa) lesions. methods: this in vivo study was conducted on 20 patients with single-rooted mandibular premolar with previously failed endodontic treatment. thi ... | 2017 | 28652899 |
| formulation and evaluation of sol-gel drug delivery system for intracanal ph sensitive controlled delivery of chlorhexidine. | persistence of viable micro-organisms even after thorough chemomechanical debridement has been cited as a major cause for endodontic failure. chlorhexidine is a drug, which has shown marked efficacy against enterococcus faecalis and candida spp., which are mostly accounted for endodontic failure and it has demonstrated high degree of substantivity to dentin by adsorption. another issue with chlorhexidine and other intracanal medicaments is the excessive or premature peaking of the drug leading t ... | 2017 | 28571266 |
| comparative evaluation of antimicrobial efficacy of triple antibiotic paste, calcium hydroxide, and a proton pump inhibitor against resistant root canal pathogens. | the objective of this study is to compare the antimicrobial efficacy of triple antibiotic paste (tap) and a proton pump inhibitor (ppi) (omeprazole) in combination with calcium hydroxide (ch) against enterococcus faecalis and candida albicans. | 2017 | 28435366 |