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quantitative evaluation of apical extrusion of intracanal bacteria using rotary protaper, k3xf, twisted and hand k-file system: an ex vivo study.the aim of this study was to evaluate the number of intracanal bacteria extruded apically during root canal preparation using rotary protaper, k3xf, twisted, and hand k-file system.201726481889
effect of passive ultrasonic irrigation on enterococcus faecalis from root canals: an ex vivo study.endodontic irrigation aims to clean and disinfect the root canal system. passive ultrasonic irrigation (pui) is based on the use of an ultrasound-activated instrument into the root canal filled with irrigant. the aim of this study was to evaluate, ex vivo, the effectiveness of pui in eliminating enterococcus faecalis from root canals. seventy-five extracted human single-root teeth were used. after root canal preparation, specimens were inoculated with e. faecalis and incubated at 37 °c for 21 da ...201726312969
biofilm forming capacity of enterococcus faecalis on gutta-percha points treated with four disinfectants using confocal scanning laser microscope: an in vitro study.the aim of this study was to evaluate and compare the in vitro biofilm forming capacity of enterococcus faecalis on gutta-percha points disinfected with four disinfectants.201726288622
antibiotic resistance pattern of enterococci isolates from nosocomial infections in a tertiary care hospital in eastern india.resistance to commonly used antibiotics by enterococci causing nosocomial infections is of concern, which necessitates judicious, responsible and evidence-based use of antibiotics. the present study was conducted to review the prevalence and identify therapeutic options for nosocomial enterococcal infections in our tertiary care hospital.201726283837
phytochemical screening, antioxidant and antibacterial activities of extracts prepared from different tissues of schinus terebinthifolius raddi that occurs in the coast of bahia, brazil.schinus terebinthifolius is widely used in traditional medicine by brazilian quilombola and indigenous communities for treatment of several diseases. extracts from different tissues are being used to produce creams to treat cervicitis and cervicovaginitis. however, most studies are limited to the assessment of the essential oils and extracts obtained from the leaves.201726246739
reduction of enterococcus faecalis in curved root canals after various sizes and tapers of canal preparation.the aim of this study was to evaluate the reduction of enterococcus faecalis in curved root canals after various sizes and tapers of the canal preparation.201726180416
enterococcus faecium and enterococcus faecalis in blood of newborns with suspected nosocomial infection.enterococci are gram-positive cocci saprophyte of the human gastrointestinal tract, diners who act as opportunistic pathogens. they can cause infections in patients hospitalized for a long time or who have received multiple antibiotic therapy. enterococcus faecalis and enterococcus faecium are the most common species in human infections. to evaluate the possibility of rapid detection of these species and their occurrence in the blood of newborns with suspected nosocomial infection, blood samples ...201724553613
ex vivo microbial leakage after using different final irrigation regimens with chlorhexidine.to assess the influence of final irrigation protocols with chlorhexidine in the coronal leakage of enterococcus faecalis in filled root canals.201723559116
enterocin as-48 as evidence for the use of bacteriocins as new leishmanicidal agents.we report the feasibility of enterocin as-48, a circular cationic peptide produced by enterococcus faecalis, as a new leishmanicidal agent. as-48 is lethal to leishmania promastigotes as well as to axenic and intracellular amastigotes at low micromolar concentrations, with scarce cytotoxicity to macrophages. as-48 induced a fast bioenergetic collapse of l. donovani promastigotes but only a partial permeation of their plasma membrane with limited entrance of vital dyes, even at concentrations bey ...201728167557
combination of ceftriaxone and ampicillin for the treatment of enterococcal endocarditis.the aim of this systematic review is to review all human trials assessing the efficacy and safety of ampicillin and ceftriaxone for enterococcal endocarditis and to discuss the clinical implications of the findings.201728166656
nipple shields in transaxillary breast augmentation.infection after breast augmentation occurs in 1.1% to 2.5% of patients. bacterial contamination of the implants could explain some complications of breast implant surgery, including infection, capsular contracture and even anaplastic large cell lymphoma. because of the evidence of bacterial spread from the nipple, nipple shields have been proposed as a routine maneuver to avoid contamination of the implants.201728166134
microbial shifts in the porcine distal gut in response to diets supplemented with enterococcus faecalis as alternatives to antibiotics.gut microbiota plays an important role in host health and nutrient digestion of animals. probiotics have become one of effective alternatives to antibiotics enhancing animal health and performance through modulating gut microbiota. previously, our research demonstrated that dietary enterococcus faecalis uc-100 substituting antibiotics enhanced growth and health of weaned pigs. to investigate the alterations of microbiota in the distal gut of pigs fed e. faecalis uc-100 substituting antibiotics, ...201728165001
screening for antibacterial and antibiofilm activities in astragalus angulosus.in a search for finding novel therapeutic agents, extracts from an endemic lebanese plant, astragalus angulosus, were evaluated for their potential in-vitro antibacterial and antibiofilm activities against three gram-positive bacterial strains; staphylococcus epidermidis (cip444), staphylococcus aureus (atcc25923), and enterococcus faecalis (atcc29212); in addition to two gram-negative strains, escherichia coli (atcc35218) and pseudomonas aeruginosa (atcc27853).201728163960
cold atmospheric pressure plasma elimination of clinically important single- and mixed-species biofilms.mixed-species biofilms reflect the natural environment of many pathogens in clinical settings and are highly resistant to disinfection methods. an indirect cold atmospheric-pressure air-plasma system was evaluated under two different discharge conditions for its ability to kill representative gram-positive (staphylococcus aureus) and gram-negative (pseudomonas aeruginosa) pathogens. plasma treatment of individual 24-h-old biofilms and mixed-species biofilms that contained additional species (ent ...201728161488
in vitro activity of oritavancin against gram-positive pathogens isolated in canadian hospital laboratories from 2011 to 2015.gram-positive bacterial pathogens isolated from patient specimens submitted to 15 canadian hospital laboratories from 2011 to 2015 were tested in the coordinating laboratory for susceptibility to oritavancin and comparative antimicrobial agents using the clinical and laboratory standards institute m07-a10 (2015) broth microdilution method. oritavancin's in vitro activity was equivalent to, or more potent than, vancomycin, daptomycin, linezolid, and tigecycline against methicillin-susceptible sta ...201728159446
synthesis, surfactant properties and antimicrobial activities of methyl glycopyranoside ethers.a series of amphiphilic methyl glucopyranoside ethers incorporating various alkyl chain lengths has been synthesized from commercially available methyl glucopyranosides following an acetalisation/hydrogenolysis sequence. the amphiphilic properties of ethers and acetal intermediates were evaluated. both families exhibit excellent surfactant properties with a maximum efficiency obtained for compounds bearing a linear dodecyl chain (cmc = 0.012 mm, γsat. = 30 mn m(-1)). antimicrobial activity studi ...201728157594
synthesis of novel pyrazinamide derivatives based on 3-chloropyrazine-2-carboxamide and their antimicrobial evaluation.aminodehalogenation of 3-chloropyrazine-2-carboxamide with variously substituted benzylamines yielded a series of fifteen 3-benzylaminopyrazine-2-carboxamides. four compounds possessed in vitro whole cell activity against mycobacterium tuberculosis h37rv that was at least equivalent to that of the standard pyrazinamide. mic values ranged from 6 to 42 μm. the best mic (6 μm) was displayed by 3-[(4-methylbenzyl)amino]pyrazine-2-carboxamide (8) that also showed low cytotoxicity in the hepg2 cell li ...201728157178
readmission rate and causes at 90-day after radical cystectomy in patients on early recovery after surgery protocol.background: radical cystectomy (rc) is associated with high risk of early and late perioperative complications, and readmissions. the enhanced recovery after surgery (eras) protocol has been applied to rc showing decreased hospital stay without increased morbidity. objective: to evaluate the specific causes of hospital readmissions in rc patients treated before and after adoption of an eras protocol at our institution. methods: we retrospectively evaluated the outcome of 207 rc patients on eras ...201728149935
sealing efficacy of mineral trioxide aggregate with and without nanosilver for root end filling: an in vitro bacterial leakage study.various materials have been added to mineral trioxide aggregate to enhance its properties. this study was aimed to compare the sealing efficacy of mta with and without nanosilver using bacterial leakage approach.201728149459
retrospective comparative analysis of cardiovascular implantable electronic device infections with and without the use of antibacterial envelopes.cardiovascular implantable electronic device (cied) infections are associated with morbidity and mortality. peri-operative systemic intravenous antibiotic prophylaxis reduces the rate of cied infections. aigisrx, a polymer envelope implanted with the cied, releases minocycline and rifampin, and has been introduced to reduce infections.201728131641
diversity of enterococcus faecalis genotypes from multiple oral sites associated with endodontic failure using repetitive sequence-based polymerase chain reaction and arbitrarily primed polymerase chain reaction.the aim of this study was to evaluate the diversity and similarity of enterococcus faecalis genotype isolates from multiple oral sites using repetitive sequence-based polymerase chain reaction and arbitrarily primed polymerase chain reaction (ap-pcr).201728131414
[update on antibiotic resistance in gram-positive bacteria].antimicrobial resistance among gram-positive bacteria, especially in staphylococcus aureus, enterococcus faecium, enterococcus faecalis, and streptococcus pneumoniae, is a serious threat to public health. these microorganisms have multiple resistance mechanisms to agents currently used in clinical practice. many of these resistance mechanisms are common to all 4 of these bacterial species, but other mechanisms seem to be more specific. the prevalence and dissemination of these mechanisms varies ...201728129816
antimicrobial activity of endodontic medicaments and vehicles using agar well diffusion method on facultative and obligate anaerobes.the aim of this study was to determine the relative antimicrobial effectiveness of these endodontic medicaments and various vehicles using an agar well diffusion assay.201728127166
a comparison of antibacterial and antibiofilm efficacy of phenothiazinium dyes between gram positive and gram negative bacterial biofilm.antimicrobial photodynamic therapy (apdt) is a process that generates reactive oxygen species (ros) in presence of photosensitizer, visible light and oxygen which destroys the bacterial cells. we investigated the photoinactivation efficiency of phenothiazinium dyes and the effect of ros generation on gram positive and gram negative bacterial cell as well as on biofilm.201728119141
growth, biogenic amine production and tyrdc transcription of enterococcus faecalis in synthetic medium containing defined amino acid concentrations.the tyraminogenic potential of the strains enterococcus faecalis ef37 and atcc 29212 was investigated in a synthetic medium containing defined amounts of tyrosine and phenylalanine at different temperatures.201728117533
catheter-associated urinary tract infection (cauti) after term cesarean delivery: incidence and risk factors at a multi-center academic institution.the purpose of this study is to identify the rate of catheter-associated urinary tract infection (cauti) after cesarean delivery (cd) and to determine if any factors increase risk of infection.201728114875
comparative efficacies of daptomycin, vancomycin, and linezolid in experimental enterococcal peritonitis.enterococci have become increasingly important pathogens for nosocomial infection (e.g. bacteremia, intra-abdominal infection, endocarditis, etc.), related to their intrinsic resistance to many antibiotics. although the in vitro susceptibility of daptomycin (dap) against enterococci is well established, the food and drug administration has only approved its use for complicated skin and skin structure infections induced by enterococcus faecalis. in this study we evaluated the potential therapeuti ...201728108098
antibacterial activity, computational analysis and host toxicity study of thymol-sulfonamide conjugates.methicillin-resistant staphylococcus aureus (mrsa) and vancomycin resistant enterococcus faecalis (vre) are notorious pathogenic multidrug resistant (mdr) bacteria in both hospital and community sectors, and today the first antibacterial drug sulfamethoxazole is ineffective. the monoterpene phenol, thymol was conjugated with seven sulfa drug derivatives individually, adopting the dye-azo synthesis protocol, and conjugates were characterized using spectral analysis techniques such as, uv, ftir, m ...201728107695
draft genome sequence of an enterococcus faecalis atcc 19433 siphovirus isolated from raw domestic sewage.we previously isolated and characterized an enterococcus faecalis atcc 19433 siphovirus from raw domestic sewage as a viral indicator of human fecal pollution. here, we report the draft genome sequence of this bacteriophage.201728104647
model systems for the study of enterococcal colonization and infection.enterococcus faecalis and enterococcus faecium are common inhabitants of the human gastrointestinal tract, as well as frequent opportunistic pathogens. enterococci cause a range of infections including, most frequently, infections of the urinary tract, catheterized urinary tract, bloodstream, wounds and surgical sites, and heart valves in endocarditis. enterococcal infections are often biofilm-associated, polymicrobial in nature, and resistant to antibiotics of last resort. understanding enteroc ...201728102784
interspecies transfer of vancomycin, erythromycin and tetracycline resistance among enterococcus species recovered from agrarian sources.enterococci are now well recognised for their ability to transfer antibiotic resistance and for their association with nosocomial infections, but less is known regarding their relevance in the wider environment. enterococcus faecalis and enterococcus faecium were isolated from a range of agrarian associated sources (low-flow water, septic tank, poultry litter, high flow water, slurry/soil) and were assessed for latent ability to transfer antimicrobial resistance.201728100194
antibacterial efficacy of several intracanal medicaments for endodontic therapy.the antibacterial efficacy of intracanal medicaments for 2 and 7 days, in open apex root canals contaminated with enterococcus faecalis biofilms was compared. one hundred and thirty-eight standardized uniradicular human roots were inoculated with e. faecalis. colony forming units (cfu) were recorded before and after medication. samples were divided in two (2/7 days), and subdivided (n=8/group): triple antibiotic paste (tap); double antibiotic paste (dap); dental base paste (dbp), consisting of c ...201728228624
genotypic characterization of vancomycin-resistant enterococcus spp. in tertiary center, iran.enterococci infection rate, mortality and morbidity has been increased in recent decades accompanied by progressive emerging antimicrobial resistance. vancomycin-resistant enterococci (vre), important pathogen in hospitalized patients, have distinct antibiotic susceptibility to glycopeptides that varied genetically and can influence in choosing therapeutic agents. in this study, we aimed to determine the patterns of antimicrobial resistance according to the genotypic variations in vres.201728228089
requirement of the crors two-component system for resistance to cell-wall-targeting antimicrobials in enterococcus faecium.enterococci are serious opportunistic pathogens that are resistant to many cell-wall-targeting antibiotics. the crors two-component signaling system responds to antibiotic-mediated cell-wall stress and is critical for resistance to cell-wall-targeting antibiotics in enterococcus faecalis here, we identify and characterize an orthologous two-component system found in enterococcus faecium that is functionally equivalent to the crors system of e. faecalis deletion of crors in e. faecium resulted in ...201728223383
comparison of methods for identifying causative bacterial microorganisms in presumed acute endophthalmitis: conventional culture, blood culture, and pcr.identification of bacterial pathogens in endophthalmitis is important to inform antibiotic selection and treatment decisions. hemoculture bottles and polymerase chain reaction (pcr) analysis have been proposed to offer good detection sensitivity. this study compared the sensitivity and accuracy of a blood culture system, a pcr approach, and conventional culture methods for identification of causative bacteria in cases of acute endophthalmitis.201728222703
technological properties and biogenic amines production by bacteriocinogenic lactococci and enterococci strains isolated from raw goat's milk.technological properties and biogenic amine production were analyzed in 56 bacteriocinogenic lactococci and enterococci strains isolated from raw goat's milk. fifteen lactococci strains were able to reduce milk ph to 5.3 or lower after 6 h, while enterococci strains were initially slow in producing acids. lactococcus lactis subsp. lactis glc06 and three strains of enterococcus faecalis (gen20, gen22, and gen23) presented high proteolytic activity. l. lactis subsp. lactis glc06 and e. faecalis ge ...201728221886
conjugative type iv secretion in gram-positive pathogens: trag, a lytic transglycosylase and endopeptidase, interacts with translocation channel protein tram.conjugative transfer plays a major role in the transmission of antibiotic resistance in bacteria. pip501 is a gram-positive conjugative model plasmid with the broadest transfer host-range known so far and is frequently found in enterococcus faecalis and enterococcus faecium clinical isolates. the pip501 type iv secretion system is encoded by 15 transfer genes. in this work, we focus on the virb1-like protein trag, a modular peptidoglycan metabolizing enzyme, and the virb8-homolog tram, a potenti ...201728219792
study on the excision and integration mediated by class 1 integron in enterococcus faecalis.recognized as a mobile genetic element, integron is correlated to the excision and integration of exogenous genes, especially bacterial resistance genes. however, most of the investigations focused on gram-positive bacteria with few exceptions. in this study, enterococcus faecalis was selected to investigate the excision and integration of class 1 integron. a total of eight plasmids were subjected to establish the transformants for excision and integration test. as results showed, positive excis ...201728390978
stimulatory effects of heat-killed enterococcus faecalis on cell-mediated immunity in fish.intracellular bacterial and viral diseases are widespread in the aquaculture industry and cause serious economic losses. development of effective vaccines and adjuvants that can induce cell-mediated immunity is urgently needed for prevention of these diseases. here we report the immunostimulatory effects of probiotic bacteria ''e. faecalis'' in ginbuna crucian carp carassius auratus langsdorfii. intraperitoneal injection of heat-killed e. faecalis induced an increase in cd4-1(+) lymphocytes, cd8 ...201728389237
antagonistic activity of lactobacillus reuteri strains on the adhesion characteristics of selected pathogens.adhesion ability of probiotics is the key factor that decides their colonization in the gastrointestinal tract and potential to inhibit pathogens. therefore, adhesion ability can be considered as a key determinant for probiotic efficacy. presents study documents the antagonistic activity of viable/untreated, lithium chloride (licl) treated or heat-killed forms of eight probiotic lactobacillus reuteri strains on the adhesion characteristics of selected pathogens. all strains investigated were abl ...201728377765
poly(methyl methacrylate) surface modification for surfactant-free real-time toxicity assay on droplet microfluidic platform.microfluidic droplet reactors have many potential uses, from analytical to synthesis. stable operation requires preferential wetting of the channel surface by the continuous phase which is often not fulfilled by materials commonly used for lab-on-chip devices. here we show that a silica nanoparticle (sinp) layer coated onto a poly(methyl methacrylate) (pmma) and other thermoplastics surface enhances its wetting properties by creating nanoroughness, and allows simple grafting of hydrocarbon chain ...201728375597
identification of the cfr methyltransferase gene in enterococcus faecalis isolated from swine: first report in brazil. 201728274847
effectiveness of persea major kopp (lauraceae) extract against enterococcus faecalis: a preliminary in vitro study.persea major kopp (lauraceae) is a plant with wound healing, antibacterial, and analgesic properties. the aim of this study was to assess the in vitro antibacterial activity of the concentrated crude extract (cce) and ethyl acetate fraction (eaf) of this plant against enterococcus faecalis and compare it with calcium hydroxide [ca(oh)2] paste and 2% chlorhexidine digluconate (chx).201728264708
susceptibility to disinfectants in antimicrobial-resistant and -susceptible isolates of escherichia coli, enterococcus faecalis and enterococcus faecium from poultry - esbl / ampc-phenotype of escherichia coli is not associated with resistance to a quaternary ammonium compound, ddac.the spread of bacteria that are simultaneously resistant to disinfectants and antimicrobials would constitute an unsettling scenario. in order to explore an association between antimicrobial resistance and reduced susceptibility to biocides / microbicides like disinfectants in agriculture, we investigated escherichia (e.) coli (n = 438) and enterococci (n = 120) isolated from six different flocks of the same poultry farm with known history of antimicrobial treatment.201728261951
antibacterial efficacy of octenisept, alexidine, chlorhexidine, and sodium hypochlorite against enterococcus faecalis biofilms.the purpose of this study was to evaluate the antibacterial effectiveness of octenisept (oct; schülke & mayr gmbh, norderstedt, germany), 1% alexidine (alx) (santa cruz biotechnology, inc, santa cruz, ca), and 2% chlorhexidine (chx) against enterococcus faecalis biofilm using confocal laser scanning microscopy.201728258812
beneficial and safety properties of a corynebacterium vitaeruminis strain isolated from the cow rumen.corynebacterium vitaeruminis mru4 was isolated from the cow rumen and was differentiated from other isolates by rep-pcr and rapd and identified by 16s rrna sequencing. this strain presented higher survival rates for low ph and bile salts treatments, and it was able to survive and multiply in simulated gastric and intestinal environments. c. vitaeruminis mru4 had a 53.2% auto-aggregation rate, 42.4% co-aggregation rate with listeria monocytogenes scott a, 41.6% co-aggregation rate with enterococc ...201728258546
evaluation of 4% sodium hypochlorite in eliminating enterococcus faecalis from the root canal when used with three irrigation methods: an in vitro study.endodontic treatment removes all pathogens, such as enterococcus faecalis from pulp and root canals. the aim of this study is to assess the usefulness of sodium hypo-chlorite (naocl) in removing e. faecalis from the root canal used with three different irrigation methods.201728258267
antimicrobial efficacy of mixtures of nanosilver and zinc oxide eugenol against enterococcus faecalis.this study aimed to assess the antimicrobial efficacy of 0, 0.5, 2, and 5 wt% nanosilver in conjunction with zinc oxide eugenol (zoe) against enterococcus faecalis.201728258260
genetic diversity of enterococcus faecalis isolated from environmental, animal and clinical sources in malaysia.enterococcus faecalis ranks as one of the leading causes of nosocomial infections. a strong epidemiological link has been reported between e. faecalis inhabiting animals and environmental sources. this study investigates the genetic diversity, antibiotic resistance and virulence determinants in e. faecalis from three sources in malaysia. a total of 250 e. faecalis isolates were obtained consisting of 120 isolates from farm animals, 100 isolates from water sources and 30 isolates from hospitalize ...201728254461
[effects of epigallocatechin-3-gallate modification on the bonding stability of an etch-and-rinse adhesive to intraradicular dentin].objective: to evaluate the effects of epigallocatechin-3-gallate (egcg) modification on the bonding stability of an etch-and-rinse adhesive to intraradicular dentin, and to find a new strategy to improve the stability of bonding interface. methods: egcg was incorporated into single bond 2 (sb2) with the concentration of 200 mg/l and 400 mg/l respectively to fabricated experimental adhesives group a and group b, while single bond 2 without egcg was used as control group. laser scanning confocal m ...201728253588
discovery of substituted oxadiazoles as a novel scaffold for dna gyrase inhibitors.dna gyrase and topoisomerase iv are type iia topoisomerases that are essential bacterial enzymes required to oversee the topological state of dna during transcription and replication processes. their atpase domains, gyrb and pare, respectively, are recognized as viable targets for small molecule inhibitors, however, no synthetic or natural product gyrb/pare inhibitors have so far reached the clinic for use as novel antibacterial agents, except for novobiocin which was withdrawn from the market. ...201728246042
confocal laser scanning, scanning electron, and transmission electron microscopy investigation of enterococcus faecalis biofilm degradation using passive and active sodium hypochlorite irrigation within a simulated root canal model.root canal irrigation is an important adjunct to control microbial infection. the aim of this study was to investigate the effect of 2.5% (wt/vol) sodium hypochlorite (naocl) agitation on the removal, killing, and degradation of enterococcus faecalis biofilm. a total of 45 root canal models were manufactured using 3d printing with each model comprising an 18 mm length simulated root canal of apical size 30 and taper 0.06. e. faecalis biofilms were grown on the apical 3 mm of the models for 10 da ...201728244230
enterococcus faecalis uses a pts permease and a host colonization-related abc transporter for maltodextrin uptake.maltodextrin is a mixture of maltooligosaccharides, which are produced by the degradation of starch or glycogen. they are mostly composed of α-1,4- and some α-1,6- linked glucose residues. genes presumed to code for the enterococcus faecalis maltodextrin transporter were induced during enterococcal infection. we therefore carried out a detailed study of maltodextrin transport in this organism. depending on their length (3 to 7 glucose residues), e. faecalis takes up maltodextrins either via malt ...201728242718
adjuvant antibiotic activity of acidic sophorolipids with potential for facilitating wound healing.the sophorolipid class of biosurfactants are finding increasing use in personal care as well as pharmaceutical products and have the potential to disrupt biofilm formation and inhibit growth in a variety of clinically relevant organisms. in order to investigate potential biomedical applications of sophorolipids derived from non-pathogenic organisms, we fractionated and purified glycolipid biosurfactant sophorolipids produced by the yeast starmerella bombicola, which yielded both acidic c 18-1 ac ...201728242666
loop-mediated isothermal amplification label-based gold nanoparticles lateral flow biosensor for detection of enterococcus faecalis and staphylococcus aureus.the report describes a simple, rapid and sensitive assay for visual and multiplex detection of enterococcus faecalis and staphylococcus aureus based on multiple loop-mediated isothermal amplification (mlamp) and lateral flow biosensor (lfb). detection and differentiation of the ef0027 gene (e. faecalis-specific gene) and nuc gene (s. aureus-specific gene) were determined using fluorescein (fitc)-and digoxin-modified primers in the mlamp process. in the presence of biotin- and fitc-/digoxin-modif ...201728239371
genetic diversity and antibiotic resistance of enterococcus faecalis isolates from traditional korean fermented soybean foods.eighty-five enterococcus faecalis isolates collected from animals (40 isolates), meju (korean fermented soybean product; 27 isolates), humans (10 isolates), and various environmental samples (eight isolates) were subjected to multilocus sequence typing (mlst) to identify genetic differences between samples of different origins. mlst analysis resulted in 44 sequence types (sts), and the eburst algorithm clustered the sts into 21 clonal complexes (ccs) and 17 singletons. the predominant sts, st695 ...201728237994
efficacy of rifampicin combination therapy for the treatment of enterococcal infections assessed in vivo using a galleria mellonella infection model.enterococci are a leading cause of healthcare-associated infection worldwide and display increasing levels of resistance to many of the commonly used antimicrobials, making treatment of their infections challenging. combinations of antibiotics are occasionally employed to treat serious infections, allowing for the possibility of synergistic killing. the aim of this study was to evaluate the effects of different antibacterial combinations against enterococcal isolates using an in vitro approach a ...201728235571
development of a rapid identification method for the differentiation of enterococcus species using a species-specific multiplex pcr based on comparative genomics.enterococci are lactic acid bacteria that are commonly found in food and in animal gut. since 16 s ribosomal rna (rrna) sequences, genetic markers for bacterial identification, are similar among several enterococcus species, it is very difficult to determine the correct species based on only 16 s rrna sequences. therefore, we developed a rapid method for the identification of different enterococcus species using comparative genomics. we compared 38 genomes of 13 enterococcus species retrieved fr ...201728229213
biological activities of undescribed north american lichen species.lichens provide a large array of compounds with the potential for pharmaceutical development. in the present study, extracts from three previously undescribed north american lichen species were examined for antioxidant, antibacterial and anticancer activities.201728369962
antimicrobial activity and chemical composition of "kpètè-kpètè": a starter of benin traditional beer tchoukoutou.the aim of this study was to investigate the antibacterial effect of the crude starter "kpètè-kpètè" and lactic acid bacteria used during the production of "tchoukoutou." to achieve this, a total of 11 lactic acid bacteria and 40 starter samples were collected from four communes. the samples were tested on 29 gram + and - strains by disk diffusion method. the minimum inhibitory and bactericidal concentrations of starter and lactic acid bacteria were determined by conventional methods. organic ac ...201728367445
vitreous hemorrhage as an early sign of acute bacterial endophthalmitis following intravitreal ranibizumab injection.to report a case of acute bacterial endophthalmitis after antivascular endothelial growth factor injection with a rare presentation of vitreous hemorrhage.201728365853
phosphotransferase systems in enterococcus faecalis og1rf enhance anti-stress capacity in vitro and in vivo.phosphotransferase systems are common and essential in bacteria, which are in charge of sugar transportation and phosphorylation. however, phosphotransferase systems were found in recent years to be associated with environmental stress factors. this study investigated the role of the mannose/fructose/sorbose phosphotransferase systems in enterococcus faecalis og1rf in adaption to harsh environments by construction of pts mutants. more than one mannose/fructose/sorbose phosphotransferase system w ...201728365379
[bactericidal effects and smear layer removal of er:yag-laser radiation against enterococcus faecalis in root canals].to evaluate the cleaning ability of er:yag laser against enterococcus faecalis in root canals.
 methods: the single-rooted human teeth were sterilized and inoculated with enterococcus faecalis and were randomly assigned into 2 groups. a group of teeth was irrigated with saline during root canal preparation while another group with naclo. after mechanical preparation, the two groups were randomly divided into 3 subgroups according to the different treatments: samples, laser radiation, and calcium ...201728364108
chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against enterococcus faecalis biofilms.propolis obtained from bee hives is a natural substance with antimicrobial properties. it is limited by its insolubility in aqueous solutions; hence ethanol and ethyl acetate extracts of malaysian propolis were prepared. both the extracts displayed antimicrobial and anti-biofilm properties against enterococcus faecalis, a common bacterium associated with hospital-acquired infections. high performance liquid chromatography (hplc) analysis of propolis revealed the presence of flavonoids like kaemp ...201728362873
rapid molecular identification of fecal origin-colonies growing on enterococcus spp.-specific culture methods.the mei, chromocult(®) enterococci, and m-enterococcus culture-based methods used to assess water quality by the detection of enterococcus spp. were first compared in terms of sensitivity using (1) 41 different type strains of enterococcus spp. and (2) environmental colonies identified by 16s rrna sequencing. then, two specific-rtpcr assays targeting enterococcus spp. and enterococcus faecalis/faecium were tested for their ability to confirm the identity of putative enterococcal colonies. the me ...201728362305
attributable mortality of hospital-acquired bloodstream infections in ireland.to estimate the attributable mortality of hospital-acquired bloodstream infections (ha-bsi) in ireland.201728359546
prevalence and bacterial isolates of mastitis in dairy farms in selected districts of eastern harrarghe zone, eastern ethiopia.the study was conducted from november 2015 to april 2016 to estimate the prevalence of clinical and subclinical mastitis in lactating cows, to assess the associated risk factors, and to isolate the major bacterial pathogens in dairy farms in selected district of eastern harrarghe zone, eastern ethiopia. the study was carried out in 384 dairy cows based on data collection, farm visit, animal examination, california mastitis test (cmt), and isolation bacterial pathogens using standard techniques. ...201728352648
synergistic effect of haloduracin and chloramphenicol against clinically important gram-positive bacteria.the emergence of drug-resistant pathogens has triggered the search for more efficient antimicrobial agents and formulations for treatment of infections. in recent years, combination therapy has become one of the effective clinical practices in treating infections. the present study deals with the effect of haloduracin, a lantibiotic bateriocin and chloramphenicol against clinically important bacteria. the combined use of haloduracin and chloramphenicol resulted in remarkable synergy against a sp ...201728352561
good bugs vs bad bugs: evaluation of inhibitory effect of selected probiotics against enterococcus faecalis.the main goal of endodontics is the prevention of apical periodontitis. this is due to the presence of persistent pathogenic microorganisms, such as enterococcus faecalis, and its ability to directly cause acute and chronic inflammation in the periapical tissues. lactobacillus has been shown to promote health in the intestines as well as to inhibit the growth of certain problematic oral bacteria. this study explores shifting the established paradigm of endodontic treatment, which has focused on ...201728349910
bacterial colonization in the apical part of extracted human teeth following root-end resection and filling: a confocal laser scanning microscopy study.the purpose of this study was to evaluate enterococcus faecalis colonization at the apical part of root canals following root-end resection and filling using confocal laser scanning microscopy (clsm).201728349219
primary pyogenic ventriculitis caused by neisseria meningitidis: case report and review of the literature.background. pyogenic ventriculitis is a well-known complication of meningitis, brain abscesses and intraventricular drains. primary pyogenic ventriculitis is a rare entity and few cases have been described so far. we report the first case of primary pyogenic ventriculitis in an adult caused by neisseria meningitidis and present an overview of all reported adult primary pyogenic ventriculitis cases in the english literature. methods. a pubmed search was performed using the terms ependymitis, vent ...201728348798
in vitro evaluation of the antibacterial activity of five sealers used in root canal therapy.antibacterial activity is one of the important characteristics of an ideal root canal sealer. the aim of this study was to investigate the antibacterial activity of five different sealers against enterococcus faecalis using two different methods.201728348620
characterization of vancomycin-resistant enterococcus faecalis and enterococcus faecium isolated from fresh produces and human fecal samples.increased enterococcal infections in hospitals and multidrug-resistant and vancomycin-resistant enterococci (vre) isolated from humans, animals, and food sources raised public health concern on the presence of vre in multiple sources. we performed a comparative analysis of the antimicrobial resistance and genetics of vre isolates derived from fresh produce and human fecal samples. of 389 enterococcus isolates, 8 fecal and 3 produce isolates were resistant to vancomycin and teicoplanin; all harbo ...201728346839
extraction of chitosan and its oligomers from shrimp shell waste, their characterization and antimicrobial effect.the present study was performed to utilize the shrimp shell waste for chitin and chitosan production, characterization by fourier transform infrared (ft-ir) technique and to evaluate the antimicrobial effects of chitosan oligomers produced by depolymerization of chitosan by nitrous acid.201728344399
distribution of antimicrobial resistance determinants, virulence-associated factors and crispr loci in isolates of enterococcus faecalis from various settings and genetic lineages.enterococcus faecalis represents an important factor of hospital-associated infections (hais). the knowledge on its evolution from a commensal to an opportunistic pathogen is still limited, thus we performed a study to characterize distribution of factors that may contribute to this adaptation. using a collection obtained from various settings (hospitalized patients, community carriers, animals, fresh food, sewage, water) we investigated differences in antimicrobial susceptibility, distribution ...201728334141
cutaneous burn injury modulates urinary antimicrobial peptide responses and the urinary microbiome.characterization of urinary bacterial microbiome and antimicrobial peptides after burn injury to identify potential mechanisms leading to urinary tract infections and associated morbidities in burn patients.201728333758
ecological impact of ciprofloxacin on commensal enterococci in healthy volunteers.the ecological impact of ciprofloxacin on commensal enterococci is unknown.201728333351
high incidence of virulence factors among clinical enterococcus faecalis isolates in southwestern iran.over the past two decades, enterococci have emerged as an important agent responsible for hospital acquired infection. several virulence factors contribute to the adherence, colonization, evasion of the host immune response, and pathogenicity and severity of the infection. enterococcus faecalis is the most common and virulent species causing infections in hospitalized patients. the aim of the present study was to examine the prevalence of genes encoding virulence factors and antimicrobial resist ...201728332345
porphyromonas gingivalis is the most abundant species detected in coronary and femoral arteries.an association between oral bacteria and atherosclerosis has been postulated. a limited number of studies have used 16s rna gene sequencing-based metagenomics approaches to identify bacteria at the species level from atherosclerotic plaques in arterial walls. the objective of this study was to establish detailed oral microbiome profiles, at both genus and species level, of clinically healthy coronary and femoral artery tissues from patients with atherosclerosis. tissue specimens were taken from ...201728326156
first canadian case report of kidney transplantation from an hiv-positive donor to an hiv-positive recipient.kidney transplantation has become standard of care for carefully selected patients living with human immunodeficiency virus (hiv) and end-stage renal disease (esrd) in the highly active antiretroviral therapy (haart) era. american and european prospective cohort studies have reported similar patient and graft survival compared with hiv-negative kidney transplant recipients. despite an increased rate of acute rejection, partially due to drug interactions, hiv immunovirologic parameter generally r ...201728321326
negative allosteric regulation of enterococcus faecalis small alarmone synthetase relq by single-stranded rna.the alarmone nucleotides guanosine pentaphosphate (pppgpp) and tetraphosphate (ppgpp), collectively referred to as (p)ppgpp, are key regulators of bacterial growth, stress adaptation, pathogenicity, and antibiotic tolerance. we show that the tetrameric small alarmone synthetase (sas) relq from the gram-positive pathogen enterococcus faecalis is a sequence-specific rna-binding protein. relq's enzymatic and rna binding activities are subject to intricate allosteric regulation. (p)ppgpp synthesis i ...201728320944
efficacy of telavancin alone and in combination with ampicillin in a rat model of enterococcus faecalis endocarditis.we first assessed telavancin (tlv) pharmacokinetics after a single subcutaneous dose of 35 mg/kg. the pharmacokinetic data were used to predict a tlv dose that simulates human exposure and the efficacy of tlv was then evaluated using tlv 21 mg/kg q 12 h against enterococcus faecalis og1rf (tlv mic of 0.06 μg/ml) in a rat endocarditis model with an indwelling catheter. therapy was given for 3 days with tlv, daptomycin (dap) or ampicillin (amp) monotherapy and with combinations of tlv plus amp, am ...201728320712
effect of using different vehicles on the physicochemical, antimicrobial, and biological properties of white mineral trioxide aggregate.this study evaluated the physicochemical, antimicrobial, and biological properties of white mineral trioxide aggregate (mta) mixed with aqueous and propylene glycol extracts of arctium lappa l. and casearia sylvestris sw.201728320540
biofilm formation capability of enterococcal strains causing urinary tract infection vis-a-vis colonisation and correlation with enterococcal surface protein gene.data regarding differences in biofilm formation among urinary isolates of enterococci causing nosocomial infection versus asymptomatic colonisation is lacking. conflicting data are available for the role of enterococcal surface protein (esp) gene in the development of enterococcal biofilms.201728303818
draft genome sequence of a multidrug-resistant strain of enterococcus faecalis, pm01, isolated from the nest of an american bushtit, psaltriparius minimus.pathogenic microorganisms associated with avian nests may detrimentally impact parental health and nest success for the nest primary users, potentially neighboring avian or terrestrial species, including humans. here, we report the genome sequence of enterococcus faecalis strain pm01, isolated from a failed nest of american bushtits, psaltriparius minimus.201728302771
purulent pericarditis as a complication of bacteraemic enterococcus faecalis urinary tract infection.purulent pericarditis is a rare clinical entity in the modern antibiotic era. the most common portal of entry is thought to be direct extension from a primary lung source and is usually caused by staphylococcus aureus, streptococcus pneumoniae or haemophilus influenzae we report the case of a man aged 69 years who presented with purulent pericarditis due to enterococcus faecalis likely caused by haematogenous spread from a urinary tract source. urgent pericardiocentesis was vital and restored hi ...201728298381
the impact of a 940 nm diode laser with radial firing tip and bare end fiber tip on enterococcus faecalis in the root canal wall dentin of bovine teeth: an in vitro study.this in vitro study aimed to compare the bactericidal effect of two different laser delivery systems, a radial firing tip (rft) and bare end fiber tip (bft) used with the 940 nm diode laser on enterococcus faecalis inoculated onto bovine radicular dentin.201728294701
successful salvage treatment of native valve enterococcus faecalis infective endocarditis with telavancin: two case reports.infective endocarditis (ie) one-year mortality rates approach 40%. here, we report two native valve enterococcus faecalis ie cases in patients successfully treated with telavancin. an 88-year-old with mitral valve endocarditis and a penicillin allergy, initially treated with intravenous vancomycin, was switched to telavancin. a 69-year-old, who previously received amoxicillin and intravenous vancomycin for presumed enterococcal bacteraemia, was diagnosed with dual valve endocarditis for which he ...201728293958
sonication of arthroplasty implants improves accuracy of periprosthetic joint infection cultures.there is evidence that sonication of explanted prosthetic hip and knee arthroplasty components with culture of the sonication fluid may enhance diagnostic sensitivity. previous studies on the use of implant sonicate cultures have evaluated diagnostic thresholds but did not elaborate on the clinical importance of positive implant sonicate cultures in the setting of presumed aseptic revisions and did not utilize consensus statements on periprosthetic joint infection (pji) diagnosis when defining t ...201728290115
antimicrobial properties of copper nanoparticles and amino acid chelated copper nanoparticles produced by using a soya extract.this paper reports a comparison of the antibacterial properties of copper-amino acids chelates and copper nanoparticles against escherichia coli, staphylococcus aureus, and enterococcus faecalis. these copper-amino acids chelates were synthesized by using a soybean aqueous extract and copper nanoparticles were produced using as a starting material the copper-amino acids chelates species. the antibacterial activity of the samples was evaluated by using the standard microdilution method (clsi m100 ...201728286459
fosfomycin and comparator activity against select enterobacteriaceae, pseudomonas, and enterococcus urinary tract infection isolates from the united states in 2012.fosfomycin is a broad-spectrum cell wall active agent that inhibits the mura enzyme involved in peptidoglycan synthesis and is fda-approved for treatment of uncomplicated urinary tract infections (utis) caused by escherichia coli and enterococcus faecalis in women. data regarding the susceptibility of recent uti isolates to fosfomycin are limited.201728285420
detection of novel oxazolidinone and phenicol resistance gene optra in enterococcal isolates from food animals and animal carcasses.altogether 7720 enterococcus faecalis and 3939 e. faecium isolated from food animals and animal carcasses during 2003-2014 in korea were investigated to determine if linezolid-resistant (lr) enterococci (≥8μg/ml) are present. overall, 12 e. faecalis and 27 e. faecium recovered from chickens (n=32), pigs (n=6), and cattle (n=1) were resistant to linezolid and were further characterized using molecular methods most lr isolates were also resistant to chloramphenicol (97.44%) and florfenicol (92.31% ...201728284617
chemical composition, antioxidant and antibacterial activities of tamarix balansae j. gay aerial parts.a previously undescribed phenolic sulphate ester, potassium 34-dihydroxy-3-methoxybenzoic acid methyl ester-5-sulphate (1), along with nine known flavonoids, kaempferol-3-o-potassium sulphate-4',7-dimethyl ether (2), kaempferol-4',7-dimethyl ether (3), rhamnocitrin-3-o-potassium sulphate (4), rhamnocitrin (5), kaempferol (6), quercetin (7), afzelin (8), quercetin-3-o-α-l-rhamnopyranoside (9) and luteolin-3'-o-potassium sulphate (10) were isolated from the aerial parts of tamarix balansae. struct ...201728281364
ultraviolet (uv)-reflective paint with ultraviolet germicidal irradiation (uvgi) improves decontamination of nosocomial bacteria on hospital room surfaces.an ultraviolet germicidal irradiation (uvgi) generator (the torch™, clordisys solutions, inc.) was used to compare the disinfection of surface coupons (plastic from a bedrail, stainless steel, and chrome-plated light switch cover) in a hospital room with walls coated with ultraviolet (uv)-reflective paint (lumacept) or standard paint. each surface coupon was inoculated with methicillin-resistant staphylococcus aureus (mrsa) or vancomycin-resistant enterococcus faecalis (vre), placed at 6 differe ...201728278065
effect of photodynamic therapy in combination with various irrigation protocols on an endodontic multispecies biofilm ex vivo.to analyse the antibacterial effect of photodynamic therapy (pdt) in combination with various irrigation protocols on a multispecies biofilm in root canals ex vivo.201728276583
identification and prevalence of tetracycline resistance in enterococci isolated from poultry in ilishan, ogun state, nigeria.tetracycline is one of the most frequently used antibiotics in nigeria both for human and animal infections because of its cheapness and ready availability. the use of tetracycline in animal husbandry could lead to horizontal transfer of tet genes from poultry to human through the gut microbiota, especially enterococci. therefore, this study is designed to identify different enterococcal species from poultry feces in selected farms in ilishan, ogun state, nigeria, determine the prevalence of tet ...201726957873
exogenous endophthalmitis caused by enterococcus casseliflavus: a case report and discussion regarding treatment of intraocular infection with vancomycin-resistant enterococci.endophthalmitis caused by enterococci is rare, and cases involving vancomycin-resistant enterococci are even more so. due to the poor bioavailability of many antibiotics in the vitreous chamber, special considerations are required when choosing antibiotics to treat these infections. the authors report the first case of exogenous endophthalmitis caused by enterococcus casseliflavus via the unique mechanism of high-velocity water stream trauma from a toy water gun. a previously healthy four-year o ...201726744592
a 10-year profile of enterococcal bloodstream infections at a tertiary-care hospital in japan.the first aim of this study is to characterize the epidemiology of enterococcal bloodstream infections (bsis) at a japanese tertiary-care hospital. the second aim is to identify predictive factors for 30-day mortality.201728385565
safety assessment of commensal enterococci from dogs.enterococci form a complex, diverse, and very important group of bacteria from the technological and food safety aspect, or from the health-improving aspect as probiotics. generally, enterococci are considered to be of low pathogenic potential, which is associated mostly with clinical strains. in these strains, production of virulence factors as well as resistance to many antimicrobial drugs could complicate treatment of nosocomial infections. because there is a lack of information on incidence ...201728316009
maldi-tof mass spectrometry as a useful tool for identification of enterococcus spp. from wild birds and differentiation of closely related species.the aim of this study was to explore the accuracy and feasibility of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) in identifying bacteria from environmental sources, as compared to rpoa gene sequencing, and to evaluate the occurrence of bacteria of the genus enterococcus in wild birds. in addition, a phyloproteomic analysis of certain enterococcus species with spectral relationships was performed. the enterococci were isolated from 25 species of wil ...201728285496
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