Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| chitosan nanoparticles enhance the antibacterial activity of chlorhexidine in collagen membranes used for periapical guided tissue regeneration. | endodontic failure is mainly associated with the persistence of microbial infection in the root canal system and/or the periradicular area. microorganisms and their toxins located in the root canal system may trigger apical periodontitis and tissue destruction. tissue regeneration in periapical surgery by using membrane barriers has shown an improved healing and bone closure. however, bacterial membrane contamination is a main reason of failure. in this in vitro study, different brands of chlorh ... | 2016 | 26478419 |
| antimicrobial effect of the extracts from hypericum perforatum against oral bacteria and biofilm formation. | one traditional medicines, hypericum perforatum l. (hypericaceae), possesses several beneficial effects against depression, ulcers, dyspepsia, abdominal pains, burns, bacterial infections, migraine headaches, and sciatica. | 2016 | 26510970 |
| high-level aminoglycoside resistance in enterococcus faecalis and enterococcus faecium causing invasive infection: twelve-year surveillance in the minami ibaraki area. | we examined prevalence of high-level aminoglycoside resistance (hlar) in enterococcus faecalis and enterococcus faecium causing invasive infection in the minami ibaraki area. ten strains of both species each, recovered from the blood or the cerebrospinal fluid between 2003 and 2014, were randomly selected every year. high-level resistance to gentamicin (hlr-gm) and streptomycin (hlr-sm) was detected in 34% (41 of 120 strains) and 18% (21) of e. faecalis and 9% (11) and 39% (48) of e. faecium, re ... | 2016 | 26492859 |
| prototype development of the intelligent hydrogel wound dressing and its efficacy in the detection of model pathogenic wound biofilms. | the early detection of wound infection in situ can dramatically improve patient care pathways and clinical outcomes. there is increasing evidence that within an infected wound the main bacterial mode of living is a biofilm: a confluent community of adherent bacteria encased in an extracellular polymeric matrix. here we have reported the development of a prototype wound dressing, which switches on a fluorescent color when in contact with pathogenic wound biofilms. the dressing is made of a hydrat ... | 2016 | 26492095 |
| surface protein esp enhances pro-inflammatory cytokine expression through nf-κb activation during enterococcal infection. | enterococcal surface protein (esp) is encoded on a pathogenicity island in enterococcus faecalis and e. faecium and is involved in biofilm formation and binding to epithelial cells. in this study, using esp-expressing e. faecalis mmh594 and its isogenic esp-deficient strain, as well as purified esp, we show that esp is sufficient for activation of nf-κb and the subsequent production of pro-inflammatory cytokines il-1β and tnf-α in macrophages in vitro. in a mouse peritonitis model, we also show ... | 2016 | 26503704 |
| antibacterial effect of n-acetylcysteine and taurolidine on planktonic and biofilm forms of enterococcus faecalis. | this study investigated the antimicrobial activity of taurolidine and n-acetylcysteine (nac) on planktonic and biofilm enterococcus faecalis phenotypes. | 2016 | 26515652 |
| abutment coating with diamond-like carbon films to reduce implant-abutment bacterial leakage. | the influence of diamond-like carbon (dlc) films on bacterial leakage through the interface between abutments and dental implants of external hexagon (eh) and internal hexagon (ih) designs was evaluated. | 2016 | 26513267 |
| the native plasmid pml21 plays a role in stress tolerance in enterococcus faecalis ml21, as analyzed by plasmid curing using plasmid incompatibility. | to investigate the role of the native plasmid pml21 in enterococcus faecalis ml21's response to abiotic stresses, the plasmid pml21 was cured based on the principle of plasmid incompatibility and segregational instability, generating e. faecalis mutant strain ml0. the mutant and the wild strains were exposed to abiotic stresses: bile salts, low ph, h2o2, ethanol, heat, and nacl, and their survival rate was measured. we found that curing of pml21 lead to reduced tolerance to stress in e. faecalis ... | 2016 | 26519343 |
| bioconversion of methanol to value-added mevalonate by engineered methylobacterium extorquens am1 containing an optimized mevalonate pathway. | methylotrophic biosynthesis using methanol as a feedstock is a promising and attractive method to solve the over-dependence of the bioindustry on sugar feedstocks derived from grains that are used for food. in this study, we introduced and engineered the mevalonate pathway into methylobacterium extorquens am1 to achieve high mevalonate production from methanol, which could be a platform for terpenoid synthesis. we first constructed a natural operon (mve) harboring the mvas and mvae genes from en ... | 2016 | 26521242 |
| daptomycin antibiotic lock therapy for hemodialysis patients with gram-positive bloodstream infections following use of tunneled, cuffed hemodialysis catheters: retrospective single center analysis. | catheter-related blood stream infection (crbsi) is a major complication in hemodialysis patients. we assessed the efficacy of systemic daptomycin (dpt) plus dpt antibiotic lock therapy (dpt-alt) for catheter salvage in patients with gram-positive crbsis. this is a retrospective study of hemodialysis patients with tunneled and cuffed hemodialysis catheters. all patients were from a single institution in taipei and received systemic dpt plus dpt-alt for the treatment of gram-positive crbsi. succes ... | 2016 | 26549513 |
| implant placement in failed endodontic sites: a review. | dental implants may fail to osseointegrate in sites of endodontic failure. this may occur as a result colonization by various anaerobic and facultative bacterial species. if an implant is placed in a site where vegetative bacteria are residing, the implant may fail to integrate if a bacterial colonization proceeds coronally. if the implant apical cortical bone is thin or if there is an apical fenestration, the colonization may proceed through the thin or nonexistent bone through the covering muc ... | 2016 | 26550925 |
| establishment of a multi-species biofilm model to evaluate chlorhexidine efficacy. | chronic infections, for example, diabetic foot ulcers, have a large impact in terms of patient morbidity and mortality. these wounds are characterized by complex polymicrobial communities of bacteria, which may include a number of difficult-to-eradicate multidrug-resistant pathogens. | 2016 | 26597632 |
| effect of a benzalkonium chloride surfactant-sodium hypochlorite combination on elimination of enterococcus faecalis. | the purpose of this investigation was to determine the effect of a sodium hypochlorite-surfactant combination on the removal of enterococcus faecalis from infected teeth. | 2016 | 26577873 |
| effect of antimicrobials used in regenerative endodontic procedures on 3-week-old enterococcus faecalis biofilm. | we evaluated the effect of various antimicrobials used in endodontic regeneration on a 3-week-old enterococcus faecalis biofilm. | 2016 | 26577870 |
| influence of biological fluids in bacterial viability on different hospital surfaces and fomites. | the hospital environment is susceptible to bacterial contamination along with survival in fomites and surfaces, allowing dissemination of potential pathogenic strains. the present research aimed to evaluate the influence of biological fluids in bacterial viability on fomites and surfaces commonly present in nosocomial environment. | 2016 | 26577628 |
| enterococcus faecalis attenuates osteogenesis through activation of p38 and erk1/2 pathways in mc3t3-e1 cells. | to explore the role of enterococcus faecalis in the proliferation, apoptosis and differentiation of osteoblasts. | 2016 | 26572053 |
| 1h, 15n and 13c chemical shift assignment of the gram-positive conjugative transfer protein trahpip501. | conjugative transfer of dna represents the most important transmission pathway in terms of antibiotic resistance and virulence gene dissemination among bacteria. trah is a putative transfer protein of the type iv secretion system (t4ss) encoded by the gram-positive (g+) conjugative plasmid pip501. this molecular machine involves a multi-protein core complex spanning the bacterial envelope thereby serving as a macromolecular secretion channel. here, we report the near complete (1)h, (13)c and (15 ... | 2016 | 26559076 |
| screening for antimicrobial and proteolytic activities of lactic acid bacteria isolated from cow, buffalo and goat milk and cheeses marketed in the southeast region of brazil. | lactic acid bacteria (lab) can be isolated from different sources such as milk and cheese, and the lipolytic, proteolytic and glycolytic enzymes of lab are important in cheese preservation and in flavour production. moreover, lab produce several antimicrobial compounds which make these bacteria interesting for food biopreservation. these characteristics stimulate the search of new strains with technological potential. from 156 milk and cheese samples from cow, buffalo and goat, 815 isolates were ... | 2016 | 26608755 |
| virulence factors, antimicrobial resistance pattern and molecular analysis of enterococcal strains isolated from burn patients. | the enterococci are emerging as a significant cause of hospital acquired infections. the pathogenesis of enterococci is attributed to the production of virulence factors and resistance to antibiotics. the purpose of the study was to assess the prevalence of genes encoding virulence factor, antimicrobial resistance determinant and molecular characteristic of enterococci isolated from burn patients. a total of 57 enterococci isolated from wound specimens of patients with burn injury were character ... | 2016 | 26620079 |
| on-chip isothermal nucleic acid amplification on flow-based chemiluminescence microarray analysis platform for the detection of viruses and bacteria. | this work presents an on-chip isothermal nucleic acid amplification test (inaat) for the multiplex amplification and detection of viral and bacterial dna by a flow-based chemiluminescence microarray. in a principle study, on-chip recombinase polymerase amplification (rpa) on defined spots of a dna microarray was used to spatially separate the amplification reaction of dna from two viruses (human adenovirus 41, phi x 174) and the bacterium enterococcus faecalis, which are relevant for water hygie ... | 2016 | 26624222 |
| bactericidal effect of er:yag laser combined with sodium hypochlorite irrigation against enterococcus faecalis deep inside dentinal tubules in experimentally infected root canals. | this study evaluated the bactericidal effect of er:yag laser radiation combined with sodium hypochlorite (naocl) irrigation in the treatment of enterococcus faecalis deep inside dentinal tubules. the er:yag laser was activated, respectively, at 0.3, 0.5 and 1.0 w for either 20 or 30 s; 52.5 g l(-1) naocl and normal saline were used for the control groups. root canals before and after treatments were examined using scanning electron microscopy (sem). bacterial reductions both on the root canal wa ... | 2016 | 26645354 |
| effect of gaseous ozone on enterococcus faecalis biofilm-an in vitro study. | the aim of this study was to evaluate the antimicrobial effect of gaseous ozone compared to conventional methods against enterococcus faecalis. | 2016 | 26637463 |
| anti-breast cancer effects of live, heat-killed and cytoplasmic fractions of enterococcus faecalis and staphylococcus hominis isolated from human breast milk. | development of tumour that is resistant to chemotherapeutics and synthetic drugs, coupled with their life-threatening side effects and the adverse effects of surgery and hormone therapies, led to increased research on probiotics' anticancer potentials. the current study investigated the potential of live, heat-killed cells (hkc) and the cytoplasmic fractions (cf) of enterococcus faecalis and staphylococcus hominis as anti-breast cancer agents. mcf-7 cell line was treated with 25, 50, 100 and 200 ... | 2016 | 26659392 |
| the opportunistic pathogen enterococcus faecalis resists phagosome acidification and autophagy to promote intracellular survival in macrophages. | while many strains of enterococcus faecalis have been reported to be capable of surviving within macrophages for extended periods, the exact mechanisms involved are largely unknown. in this study, we found that after phagocytosis by macrophages, enterococci-containing vacuoles resist acidification, and e. faecalis is resistant to low ph. ultrastructural examination of the enterococci-containing vacuole by transmission electron microscopy revealed a single membrane envelope, with no evidence of t ... | 2016 | 26663775 |
| gram-positive cocci in dutch icus with and without selective decontamination of the oropharyngeal and digestive tract: a retrospective database analysis. | the objective of this study was to determine time trends in the rate of gram-positive cocci in 42 dutch icus that continuously used or did not use selective oropharyngeal decontamination (sod) or selective decontamination of the digestive tract (sdd) and icus that introduced sod/sdd. | 2016 | 26661393 |
| effects of salinity and temperature on inactivation and repair potential of enterococcus faecalis following medium- and low-pressure ultraviolet irradiation. | to investigate the medium-pressure (mp) and low-pressure (lp) ultraviolet (uv) susceptibility and the repair potential of enterococcus faecalis (dsm 20478) after uv treatment. | 2016 | 26669257 |
| reduction of viable enterococcus faecalis in human radicular dentin treated with 1% cetrimide and conventional intracanal medicaments. | the purpose of this study was to evaluate the viability of e. faecalis after a 14-day exposure to 1% cetrimide (cet), triantibiotic paste (i.e., metronidazole, minocycline, and ciprofloxacin), 2% chlorhexidine (chx) gel, and calcium hydroxide (ca[oh]2 ) in an infected dentine model. | 2016 | 26666992 |
| cleistochlamys kirkii chemical constituents: antibacterial activity and synergistic effects against resistant staphylococcus aureus strains. | cleistochlamys kirkii (benth) oliv., (annonaceae) is a medicinal plant traditionally used in mozambique to treat infectious diseases. | 2016 | 26674158 |
| asymptomatic bacteriuria in clinical urological practice: preoperative control of bacteriuria and management of recurrent uti. | asymptomatic bacteriuria (abu) is a common clinical condition that often leads to unnecessary antimicrobial use. the reduction of antibiotic overuse for abu is consequently an important issue for antimicrobial stewardship and to reduce the emergence of multidrug resistant strains. there are two issues in everyday urological practice that require special attention: the role of abu in pre-operative prophylaxis and in women affected by recurrent urinary tract infections (rutis). nowadays, this is t ... | 2016 | 26742080 |
| early gut colonization of preterm infants: effect of enteral feeding tubes. | the aim of the study was to evaluate the potential colonization of nosocomial bacteria in enteral feeding systems and its effect on early gut colonization of preterm neonates. | 2016 | 26741949 |
| microbial evolution of traditional mountain cheese and characterization of early fermentation cocci for selection of autochtonous dairy starter strains. | the microbial population of traditional mountain (tm) cheese was investigated and characterized for the selection of cocci suitable for developing new starter cultures. samples of milk, curd and cheese at different ripening times were enumerated in selective culture media and 640 colonies were isolated from curd and cheese after 24 h of ripening. the lactic acid bacteria (lab) isolated from m17 were clustered into 231 biotypes by rapd-pcr analysis and identified as lactococcus lactis, streptococ ... | 2016 | 26678135 |
| control of shigatoxin-producing escherichia coli in cheese by dairy bacterial strains. | bio-preservation could be a valuable way to control shigatoxin-producing escherichia coli (stec) in cheese. to this end, 41 strains were screened for their inhibitory potential on model cheese curd and on pasteurized and raw milk uncooked pressed cheeses. strains of lactococcus lactis, lactococcus garvieae, leuconostoc pseudomesenteroides, leuconostoc citreum, lactobacillus sp, carnobacterium mobile, enterococcus faecalis, enterococcus faecium, macrococcus caseolyticus and hafnia alvei reduced s ... | 2016 | 26678131 |
| substantivity of ag-ca-si mesoporous nanoparticles on dentin and its ability to inhibit enterococcus faecalis. | the main purpose of this study was to investigate the substantivity of ag-ca-si mesoporous nanoparticles (ag-mcsns) on dentin and its residual antibacterial effects against enterococcus faecalis. ag-mcsns were fabricated and characterized, ion release profile and ph were tested, and the ability to inhibit planktonic e. faecalis as well as the cytotoxicity was evaluated. dentin slices were medicated with ca(oh)2 paste, 2 % chlorhexidine gel and ag-mcsns paste for 7 days and then irrigated. dentin ... | 2016 | 26676862 |
| the bactericidal effect of 2780 and 940 nm laser irradiation on enterococcus faecalis in bovine root dentin slices of different thicknesses. | the aim of this in vitro study was to investigate the antibacterial effect of the dual wavelength (2780 nm er,cr:ysgg and 940 nm diode) laser in elimination of enterococcus faecalis in comparison with a 2780 nm er,cr:ysgg laser alone. | 2016 | 26685038 |
| tigecycline resistance in clinical isolates of enterococcus faecium is mediated by an upregulation of plasmid-encoded tetracycline determinants tet(l) and tet(m). | tigecycline represents one of the last-line therapeutics to combat multidrug-resistant bacterial pathogens, including vre and mrsa. the german national reference centre for staphylococci and enterococci has received 73 tigecycline-resistant enterococcus faecium and enterococcus faecalis isolates in recent years. the precise mechanism of how enterococci become resistant to tigecycline remains undetermined. this study documents an analysis of the role of efflux pumps in tigecycline resistance in c ... | 2016 | 26682961 |
| redefining healthy urine: a cross-sectional exploratory metagenomic study of people with and without bladder dysfunction. | we used the pathoscope platform to perform species level analyses of publicly available, 16s rrna pyrosequenced, asymptomatic urine data to determine relationships between microbiomes, and clinical and functional phenotypes. | 2016 | 26807926 |
| monitoring the effectiveness of photodynamic therapy with periodic renewal of the photosensitizer on intracanal enterococcus faecalis biofilms. | photodynamic therapy (pdt) can eliminate microorganisms in a root canal. however, the parameters for disinfection remain undefined. this study assessed the effectiveness of a pdt protocol against intracanal enterococcus faecalis biofilms. | 2016 | 26796030 |
| vinegar treatment prevents the development of murine experimental colitis via inhibition of inflammation and apoptosis. | this study investigated the preventive effects of vinegar and acetic acid (the active component of vinegar) on ulcerative colitis (uc) in mice. vinegar (5% v/v) or acetic acid (0.3% w/v) treatment significantly reduced the disease activity index and histopathological scores, attenuated body weight loss, and shortened the colon length in a murine experimental colitis model induced by dextran sulfate sodium (dss). further mechanistic analysis showed that vinegar inhibited inflammation through supp ... | 2016 | 26795553 |
| in silico driven design and synthesis of rhodanine derivatives as novel antibacterials targeting the enoyl reductase inha. | here, we report on the design, synthesis, and biological evaluation of 4-thiazolidinone (rhodanine) derivatives targeting mycobacterial tuberculosis (mtb) trans-2-enoyl-acyl carrier protein reductase (inha). compounds having bulky aromatic substituents at position 5 and a tryptophan residue at position n-3 of the rhodanine ring were the most active against inha, with ic50 values ranging from 2.7 to 30 μm. the experimental data showed consistent correlations with computational studies. their anti ... | 2016 | 26730986 |
| diversity and evolution of the tn5801-tet(m)-like integrative and conjugative elements among enterococcus, streptococcus, and staphylococcus. | this work describes the diversity and evolution of tn5801 among enterococci, staphylococci, and streptococci based on analysis of the 5,073 genomes of these bacterial groups available in gene databases. we also examined 610 isolates of enterococcus (from 10 countries, 1987 to 2010) for the presence of this and other known ctn-tet(m) elements due to the scarcity of data about tn5801 among enterococci. genome location (by iceu-i-pulsed-field gel electrophoresis [pfge] hybridization/integration sit ... | 2016 | 26729505 |
| chromosomally and extrachromosomally mediated high-level gentamicin resistance in streptococcus agalactiae. | streptococcus agalactiae (group b streptococcus [gbs]) is a leading cause of sepsis in neonates. the rate of invasive gbs disease in nonpregnant adults also continues to climb. aminoglycosides alone have little or no effect on gbs, but synergistic killing with penicillin has been shown in vitro. high-level gentamicin resistance (hlgr) in gbs isolates, however, leads to the loss of a synergistic effect. we therefore performed a multicenter study to determine the frequency of hlgr gbs isolates and ... | 2016 | 26729498 |
| antibiotic susceptibility profiles of dairy leuconostoc, analysis of the genetic basis of atypical resistances and transfer of genes in vitro and in a food matrix. | in spite of a global concern on the transfer of antibiotic resistances (ar) via the food chain, limited information exists on this issue in species of leuconostoc and weissella, adjunct cultures used as aroma producers in fermented foods. in this work, the minimum inhibitory concentration was determined for 16 antibiotics in 34 strains of dairy origin, belonging to leuconostoc mesenteroides (18), leuconostoc citreum (11), leuconostoc lactis (2), weissella hellenica (2), and leuconostoc carnosum ... | 2016 | 26726815 |
| gentamicin induces efaa expression and biofilm formation in enterococcus faecalis. | enterococci have been ranked among the leading causes of nosocomial bacteremia and urinary tract infection. this study aimed to investigate the effect of ampicillin, vancomycin, gentamicin and ceftizoxime on biofilm formation and gene expression of colonization factors on enterococcus faecalis. twelve clinical isolates of e. faecalis were used to investigate the effect of antibiotics on biofilm formation and gene expression of efaa, asa1, ebpa, esp and ace. flow system assay and microtiter plate ... | 2016 | 26724739 |
| effectiveness of edta and modified salt solution to detach and kill cells from enterococcus faecalis biofilm. | disruption of the matrix of endodontic biofilms will aid in their removal from a root canal. therefore, the aim of this study was to investigate the efficacy of edta and a modified salt solution (mss) to detach bacteria from biofilms. | 2016 | 26723483 |
| first identification of tn916-like element in industrial strains of lactobacillus vini that spread the tet-m resistance gene. | the open process used to ferment sugar cane juice or molasses to produce ethanol fuel is prone to contamination by bacterial cells of different species, in particular lactobacilli. the situation can be exacerbated by the emergence of resistant cells to industrial antibiotics that are normally used to combat this contamination. in this work, two lactobacillus vini isolates from ethanol distilleries were identified and found to be resistant to doxycycline, a tetracycline derivative, although sensi ... | 2016 | 26722009 |
| discordance between perioperative antibiotic prophylaxis and wound infection cultures in patients undergoing pancreaticoduodenectomy. | wound infections after pancreaticoduodenectomy (pd) are common. the standard antibiotic prophylaxis given to prevent the infections is often a cephalosporin. however, this decision is rarely guided by microbiology data pertinent to pd, particularly in patients with biliary stents. | 2016 | 26720272 |
| synthesis and biological evaluation of 2-aminobenzothiazole and benzimidazole analogs based on the clathrodin structure. | a series of 2-aminobenzothiazole and benzimidazole analogs based on the clathrodin scaffold was synthesized and investigated for their antimicrobial and antiproliferative activities as well as for their effects in hepatitis c virus (hcv) replicon model. compound 7, derived from 2-aminobenzothiazole, exhibited moderate antimicrobial activity only against the gram-positive bacterium, enterococcus faecalis. in the antiviral assay, compounds 4d and 7 were found to suppress the hcv replicon by >70%, ... | 2016 | 26709468 |
| human health risks associated with antimicrobial-resistant enterococci and staphylococcus aureus on poultry meat. | enterococci and staphylococci are frequent contaminants on poultry meat. enterococcus faecalis, enterococcus faecium and staphylococcus aureus are also well-known aetiological agents of a wide variety of infections resulting in major healthcare costs. this review provides an overview of the human health risks associated with the occurrence of these opportunistic human pathogens on poultry meat with particular focus on the risk of food-borne transmission of antimicrobial resistance. in the absenc ... | 2016 | 26706616 |
| partial purification and characterization of bacteriocin produced by enterococcus faecalis du10 and its probiotic attributes. | a novel bacteriocin produced by avian duck isolated lactic acid bacterium enterococcus faecalis du10 was isolated. this bacteriocin showed a broad spectrum of antibacterial activity against important food-borne pathogens and was purified by size exclusion chromatography followed by reverse-phase high-performance liquid chromatography in a c-18 column. tricine-sds page revealed the presence of a band with an estimated molecular mass of 6.3 kda. the zymogram clearly linked the antimicrobial activi ... | 2016 | 26786752 |
| nitric oxide-releasing polyacrylonitrile disperses biofilms formed by wound-relevant pathogenic bacteria. | to test the antimicrobial and antibiofilm properties of a nitric oxide (no)-releasing polymer against wound-relevant bacterial pathogens. | 2016 | 26784529 |
| escherichia coli and enterococcus faecalis are able to incorporate and enhance a pre-formed gardnerella vaginalis biofilm. | gardnerella vaginalis is the most frequent microorganism found in bacterial vaginosis (bv), while escherichia coli and enterococcus faecalis are amongst the most frequent pathogens found in urinary tract infections (utis). this study aimed to evaluate possible interactions between utis pathogens and g. vaginalis using an in vitro dual-species biofilm model. our results showed that dual-species biofilms reached significantly higher bacterial concentration than monospecies biofilms. moreover, visu ... | 2016 | 26782142 |
| prevalence, outcome and risk factor associated with vancomycin-resistant enterococcus faecalis and enterococcus faecium at a tertiary care hospital in northern india. | to determine the prevalence, genotype, risk factors and mortality in patients having vancomycin-resistant enterococcus faecalis (vr e. faecalis) and enterococcus faecium (vr e. faecium) infection or colonisation. | 2016 | 26776117 |
| antibacterial activity and immunomodulatory effects on a bovine mammary epithelial cell line exerted by nisin a-producing lactococcus lactis strains. | twenty-nine strains of mastitis pathogens were used to study the antibacterial activity of the cell-free supernatants (cfs) of 25 strains of lactococcus lactis ssp. lactis. out of the tested strains, only the cfs of l. lactis ll11 and sl153 were active, inhibiting and killing most of the pathogens. by means of ultra-performance liquid chromatography/high resolution mass spectrometry, they were shown to produce nisin a, a class i bacteriocin. a variable sensitivity to nisin a-containing cfs was o ... | 2016 | 26774727 |
| complete genome sequence of enterococcus faecalis strain p8-1 isolated from wild magellanic penguin (spheniscus magellanicus) feces on the south coast of brazil. | enterococcus faecalis strains have a ubiquitous nature that allows them to survive in different niches. studies involving enterococci isolated from marine animals are scarce. therefore, in this study, we report the complete genome sequence of e. faecalis strain p8-1 isolated from feces of a magellanic penguin on the south coast of brazil. | 2016 | 26769928 |
| caenorhabditis elegans susceptibility to gut enterococcus faecalis infection is associated with fat metabolism and epithelial junction integrity. | gut bacteria-host interactions have been implicated in the pathogenesis of numerous human diseases, but few mechanisms have been described. the genetically tractable nematode worm caenorhabditis elegans can be infected with pathogenic bacteria, such as the human gut commensal enterococcus faecalis, via feeding, making it a good model for studying these interactions. | 2016 | 26769134 |
| fumarate reductase-producing enterococci reduce methane production in rumen fermentation in vitro. | biotic agents such as fumarate-reducing bacteria can be used for controlling methane (ch4) production in the rumen. fumarate-reducing bacteria convert fumarate to succinate by fumarate reductase, ultimately leading to the production of propionate. fumarate-reducing bacteria in the genus enterococcus were isolated from rumen fluid samples from slaughtered korean native goats. the enterococci were identified as enterococcus faecalis srod5 and e. faecium srod by phylogenetic analyses of 16s rrna ge ... | 2016 | 26767574 |
| the efficacy of photon-initiated photoacoustic streaming and sonic-activated irrigation combined with qmix solution or sodium hypochlorite against intracanal e. faecalis biofilm. | the aim of the study was to assess the antibacterial efficacy of photon-initiated photoacoustic streaming (pips) using an er:yag laser and sonic-activated irrigation combined with qmix irrigant or sodium hypochlorite against enterococcus faecalis intracanal biofilm. root canals of 91 human extracted single-canal teeth were instrumented, sterilized, contaminated with e. faecalis and incubated for 15 days. the infected teeth were then randomly distributed into six experimental groups: g1: pips/er: ... | 2016 | 26754179 |
| establishment and analysis of in vitro biomass from salvia corrugata vahl. and evaluation of antimicrobial activity. | demethylfruticuline a and fruticuline a, the most abundant compounds from the surface extract of salvia corrugata vahl., have shown antibacterial, antitumor and cytotoxic activities. in order to obtain these icetexane diterpenes from in vitro cultures of s. corrugata, protocols were developed for callus production, micropropagation and shoot regeneration. analysis of the regenerated shoots showed the presence of both icetexanes, micropropagated plants contained only fruticuline a, while the call ... | 2016 | 26753532 |
| incidence of virulence determinants in clinical enterococcus faecalis and enterococcus faecium isolates collected in bulgaria. | to evaluate the prevalence of some virulence genes among 510 clinical enterococcus spp. isolates and to assess the association of those genes with the species, infection site, and patient group (inpatients/outpatients). | 2016 | 26849965 |
| antimicrobial and cytotoxic activity of cuminum cyminum as an intracanal medicament compared to chlorhexidine gel. | the aims of this study were i) to define the chemical constituents of cuminum cyminum (cumin) essential oil, ii) to compare the antimicrobial activity of this oil to that of chlorhexidine (chx) and co-trimoxazole on planktonic and biofilm forms of bacteria isolated from the teeth with persistent endodontic infection and iii ) to compare the cytotoxicity of these medicaments on l929 fibroblasts. | 2016 | 26843877 |
| antibacterial activity of diode laser and sodium hypochlorite in enterococcus faecalis-contaminated root canals. | the aim of the present in vitro study was to evaluate the disinfection ability of 980-nm diode laser in comparison with sodium hypochlorite (naocl) as a common root canal irrigant in canals infected with enterococcus faecalis (e. faecalis). | 2016 | 26843870 |
| effectiveness of different disinfection protocols on the reduction of bacteria in enterococcus faecalis biofilm in teeth with large root canals. | this study compared the effectiveness of different disinfection protocols in reducing bacteria in an enterococcus faecalis biofilm in teeth with large root canals. | 2016 | 26830428 |
| cardiac surgery for infective endocarditis in patients with intravenous drug use. | intravenous drug users have a high risk of infective endocarditis and reduced survival. cardiac surgery may be recommended for these patients, but redo surgery is controversial. this study describes the characteristics and outcomes of intravenous drug users accepted for surgery during a 12-year period. | 2016 | 26826713 |
| γ-cyclodextrin capped silver nanoparticles for molecular recognition and enhancement of antibacterial activity of chloramphenicol. | computational studies were conducted to identify the favourable formation of the inclusion complex of chloramphenicol with cyclodextrins. the results of molecular docking and molecular dynamics predicted the strongest interaction of chloramphenicol with γ-cyclodextrin. further, the inclusion complex of chloramphenicol with γ-cyclodextrin was experimentally prepared and a phenomenon of inclusion was verified by using different characterization techniques such as thermogravimetric analysis, differ ... | 2016 | 26824520 |
| development and characterization of potent cyclic acyldepsipeptide analogues with increased antimicrobial activity. | the problem of antibiotic resistance has prompted the search for new antibiotics with novel mechanisms of action. analogues of the a54556 cyclic acyldepsipeptides (adeps) represent an attractive class of antimicrobial agents that act through dysregulation of caseinolytic protease (clpp). previous studies have shown that adeps are active against gram-positive bacteria (e.g., mrsa, vre, prsp (penicillin-resistant streptococcus pneumoniae)); however, there are currently few studies examining gram-n ... | 2016 | 26818454 |
| enterococcus faecium small colony variant endocarditis in an immunocompetent patient. | small colony variants (scv) are slow-growing subpopulations of bacteria usually associated with auxotrophism, causing persistent or recurrent infections. enterococcus faecalis scv have been seldom described, and only one case of enterococcus faecium scv has been reported, associated with sepsis in a leukaemia patient. here we report the first case described of bacteraemia and endocarditis by scv e. faecium in an immunocompetent patient. | 2016 | 26862434 |
| antibiofilm efficacy of honey and bee-derived defensin-1 on multi-species wound biofilm. | many clinically relevant biofilms are polymicrobial. examining the effect of antimicrobials in a multi-species biofilm consortium is of great clinical importance. the goal of this study was to investigate the effect of different honey types against bacterial wound pathogens grown in multi-species biofilm and to test the antibiofilm activity of honey defensin-1 in its recombinant form. modified lubbock chronic wound biofilm formed by four bacterial species (staphylococcus aureus, streptococcus ag ... | 2016 | 26861950 |
| field study on evaluation of the efficacy and usability of two disinfectants for drinking water treatment at small cattle breeders and dairy cattle farms. | the hygienic quality of drinking water for cattle originated from different sources together with the efficacy and usability of two types of disinfectants against waterborne pathogens were assessed for small cattle breeders and dairy cattle farms. a total of 120 drinking water samples were collected from water troughs representing three different water sources commonly used for cattle drinking (tap, underground and surface water; n = 65, 25, and 30, respectively). collected samples were cultured ... | 2016 | 26861741 |
| photoactivated disinfection (pad) of dental root canal system - an ex-vivo study. | to investigate the efficacy of photo activated disinfection (pad) in reducing colony-forming unit (cfu) counts of enterococcus faecalis (e. faecalis) in infected dental root canals. the study compared the efficacy of pad with conventional endodontic treatment (cet) and also a combination of cet along with pad. | 2016 | 26858548 |
| [in vitro activity of ampicillin-ceftriaxone against enterococcus faecalis isolates recovered from invasive infections]. | in vitro activity of the combination of ampicillin- ceftriaxone against 30 enterococcus faecalis isolates recovered from invasive infections in patients admitted to hospital de clínicas josé de san martin in the city of buenos aires was assessed. ampicillin- ceftriaxone synergies were determined by microdilution in müeller-hinton (mh) broth with and without subinhibitory concentrations of ceftriaxone. synergy was detected in 22/30 isolates. a decrease in both minimum inhibitory concentration (mi ... | 2016 | 26857425 |
| an analysis in vivo of intracanal bacterial load before and after chemo-mechanical preparation: a comparative analysis of two irrigants and two activation techniques. | the goals of this randomized double-blind trial were to assess the antimicrobial activity in vivo of sodium hypochlorite (naocl) vs. chlorhexidine gluconate (chx) used in combination either with endoactivator® or irri s® files in patients with apical periodontitis. | 2016 | 26855714 |
| the effects of silver nanoparticles on antimicrobial activity of proroot mineral trioxide aggregate (mta) and calcium enriched mixture (cem). | although, mineral trioxide aggregate (mta) and new experimental cement (cem) are good root filling cements, but had no or low antimicrobial activities. the aim of this study was to evaluate the effects of addition of silver nanoparticles (snp) to these two cements on antimicrobial effects against five most dental infection related microorganisms. | 2016 | 26855701 |
| to comparatively evaluate the antimicrobial efficacy of chlorhexidine, nisin and linezolid as an intracanal medicament on enterococcus faecalis: an in vitro study. | the present study evaluated the antimicrobial efficacy of chlorhexidine, nisin and linezolid and a control group (normal saline ) against enterococcus faecalis (ef). | 2016 | 26888241 |
| antibacterial effect of hydroalcoholic extract of punica granatum linn. petal on common oral microorganisms. | objectives. this study aimed to assess the effect of hydroalcoholic extract of punica granatum linn. (p. granatum) petal on streptococcus sanguinis, streptococcus mutans, streptococcus salivarius, streptococcus sobrinus, and enterococcus faecalis. materials and methods. in this in vitro study, p. granatum extract was prepared using powdered petals and water-ethanol solvent. antibacterial effect of the extract, chlorhexidine (chx), and ampicillin was evaluated on brain heart infusion agar (bhia) ... | 2016 | 26884763 |
| functional dissection of the crors two-component system required for resistance to cell wall stressors in enterococcus faecalis. | bacteria use two-component signal transduction systems (tcss) to sense and respond to environmental changes via a conserved phosphorelay between a sensor histidine kinase and its cognate response regulator. the opportunistic pathogen enterococcus faecalis utilizes a tcs comprised of the histidine kinase cros and the response regulator cror to mediate resistance to cell wall stresses such as cephalosporin antibiotics, but the molecular details by which crors promotes cephalosporin resistance have ... | 2016 | 26883822 |
| bacteriospermia and its impact on basic semen parameters among infertile men. | introduction. semen analysis is considered as the surrogate marker for male fecundity while assessing infertile men. there are several reasons for altered semen quality and bacteriospermia could be one among them. thereby the aim of our work is to study the semen culture and its impact on semen parameters among infertile men. materials and methods. semen samples were collected from men attending infertility clinic. semen parameters were analysed based on who guidelines. also, samples were subjec ... | 2016 | 26880908 |
| electrochemical disinfection of simulated ballast water on pbo2/graphite felt electrode. | a novel pbo2/graphite felt electrode was constructed by electrochemical deposition of pbo2 on graphite felt and characterized by x-ray powder diffraction (xrd) and scanning electron microscopy (sem) analysis. the prepared electrode is a viable technology for inactivation of escherichia coli, enterococcus faecalis, and artemia salina as indicator organisms in simulated ballast water treatment, which meets the international maritime organization (imo) regulation d-2. the effects of contact time an ... | 2016 | 26880130 |
| isoflavone metabolism by a collection of lactic acid bacteria and bifidobacteria with biotechnological interest. | almost all soy isoflavones exist as glycosides, daidzin, genistin, and glycitin. we analyzed the capacity of 92 strains of lactic acid bacteria (lab) and bifidobacteria with biotechnological interest to process the glycosylated isoflavones daidzin, genistin, and glycitin in their more bioavailable aglycones and their metabolites as dihydrodaidzein (dhd), o-desmethylangolensin, and equol. representative strains of the four genera studied lactobacillus, enterococcus, lactococcus, and bifidobacteri ... | 2016 | 26878882 |
| ampicillin in combination with ceftaroline, cefepime, or ceftriaxone demonstrates equivalent activities in a high-inoculum enterococcus faecalis infection model. | ampicillin-ceftriaxone combination therapy has become a predominant treatment for serious enterococcus faecalis infections, such as endocarditis. unfortunately, ceftriaxone use is associated with future vancomycin-resistant enterococcus colonization. we evaluated e. faecalis in an in vitro pharmacodynamic model against simulated human concentration-time profiles of ampicillin plus ceftaroline, cefepime, ceftriaxone, or gentamicin. ampicillin-cefepime and ampicillin-ceftaroline demonstrated activ ... | 2016 | 26926624 |
| lipoteichoic acid of enterococcus faecalis inhibits the differentiation of macrophages into osteoclasts. | enterococcus faecalis is associated with persistent endodontic infection and refractory apical periodontitis. recently, we have shown that heat-killed e. faecalis attenuates osteoclast differentiation. because lipoteichoic acid (lta) is a major virulence factor of gram-positive bacteria, we investigated the effect of lta from e. faecalis (eflta) on osteoclast differentiation. | 2016 | 26920932 |
| envelope structures of gram-positive bacteria. | gram-positive organisms, including the pathogens staphylococcus aureus, streptococcus pneumoniae, and enterococcus faecalis, have dynamic cell envelopes that mediate interactions with the environment and serve as the first line of defense against toxic molecules. major components of the cell envelope include peptidoglycan (pg), which is a well-established target for antibiotics, teichoic acids (tas), capsular polysaccharides (cps), surface proteins, and phospholipids. these components can underg ... | 2016 | 26919863 |
| the impact of host metapopulation structure on the population genetics of colonizing bacteria. | many key bacterial pathogens are frequently carried asymptomatically, and the emergence and spread of these opportunistic pathogens can be driven, or mitigated, via demographic changes within the host population. these inter-host transmission dynamics combine with basic evolutionary parameters such as rates of mutation and recombination, population size and selection, to shape the genetic diversity within bacterial populations. whilst many studies have focused on how molecular processes underpin ... | 2016 | 26916623 |
| synthesis and biological activities of lanthanide (iii) nitrate complexes with n-(2-hydroxynaphthalen-1-yl) methylene) nicotinohydrazide schiff base. | the field of coordination chemistry has registered a phenomenal growth during last few decades. it is well known that precious metals have been used for medicinal purposes for at least 3500 years. at that time, precious metals were believed to benefit health because of their rarity, but research has now well established the link between medicinal properties of inorganic drugs and specific biological properties. | 2016 | 26916500 |
| in vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers. | the aim of this study was to evaluate whether the modification in the silver component is capable of providing guttaflow 2 with antibacterial activity against enterococcus faecalis compared with epoxy resin-based (ah plus) and zinc oxide and eugenol-based (endofill) sealers. the antibacterial activity was evaluated using a reference strain of e. faecalis (atcc 29212). freshly mixed sealers were subjected to the agar diffusion test (adt), while the direct contact test (dct) was performed after ma ... | 2016 | 26910015 |
| screening of the enterocin-encoding genes and antimicrobial activity in enterococcus species. | in the current study, a total of 135 enterococci strains from different sources were screened for the presence of the enterocin-encoding genes enta, entp, entb, entl50a, and entl50b. the enterocin genes were present at different frequencies, with enta occurring the most frequently, followed by entp and entb; entl50a and l50b were not detected. the occurrence of single enterocin genes was higher than the occurrence of multiple enterocin gene combinations. the 80 isolates that harbor at least one ... | 2016 | 26907753 |
| prevalence and risk factors of early fecal carriage of enterococcus faecalis and staphylococcus spp and their antimicrobial resistant patterns among healthy neonates born in a hospital setting in central saudi arabia. | to investigate the prevalence, antibiotic resistant profiles, and risk factors of early fecal carriage of enterococcus faecalis (e. faecalis) and staphylococci among 150 healthy saudi neonates born in a hospital setting in central saudi arabia. | 2016 | 26905350 |
| evolution of resistance to cationic biocides in streptococcus mutans and enterococcus faecalis. | the aim of this study was to investigate whether streptococcus mutans and enterococcus faecalis develop resistance to the cationic biocides chlorhexidine (chx), cetylpyridinium chloride (cpc), and 12-methacryloyloxydodecylpyridinium bromide (mdpb). | 2016 | 26904979 |
| genetic environment of the transferable oxazolidinone/phenicol resistance gene optra in enterococcus faecalis isolates of human and animal origin. | aim of this study was to analyse 17 non-related enterococcus faecalis isolates of human and animal origin for the genetic environment of the novel oxazolidinone/phenicol resistance gene optra. | 2016 | 26903276 |
| evaluation of polymorphisms in pbp4 gene and genetic diversity in penicillin-resistant, ampicillin-susceptible enterococcus faecalis from hospitals in different states in brazil. | the aim of the present study was to verify whether penicillin-resistant, ampicillin-susceptible enterococcus faecalis (prasef) occurred in brazil prior to the beginning of the 21st century, and to verify whether ampicillin susceptibility can predict susceptibility to other β-lactams in e. faecalis with this inconsistent phenotype. the presence of polymorphisms in the pbp4 gene and genetic diversity among the isolates were investigated. of 21 prasef analyzed, 5 (23.8%) and 4 (19.0%) were imipenem ... | 2016 | 26903013 |
| clinical characteristics and antimicrobial patterns in complicated intra-abdominal infections: a 6-year epidemiological study in southern china. | complicated intra-abdominal infection (ciais) are a common and important cause of morbidity worldwide. in this study, the clinical features, microbiological profiles, antimicrobial patterns and treatments of 3233 ciai patients (mean age, 47.6 years; 54.7% male) with 3531 hospitalisations from 2008-2013 were retrospectively investigated. the most commonly isolated bacteria were escherichia coli (47.6%), klebsiella pneumoniae (16.9%), enterococcus faecalis (10.4%) and pseudomonas aeruginosa (8.8%) ... | 2016 | 26899413 |
| comparison of telavancin and vancomycin lock solutions in eradication of biofilm-producing staphylococci and enterococci from central venous catheters. | results of a study of the activity of antibiotic lock solutions of vancomycin and telavancin against biofilm-forming strains of staphylococcus epidermidis, enterococcus faecalis, and staphylococcus aureus are reported. | 2016 | 26896504 |
| killing of vre enterococcus faecalis by commensal strains: evidence for evolution and accumulation of mobile elements in the absence of competition. | enterococci are members of the gastrointestinal tract of humans and most animals that, over the past 3 decades, have emerged as leading causes of multidrug resistant hospital acquired infection (hai). in addition to their general hardiness, many traits have entered enterococcal lineages through horizontal gene transfer, which has led to the evolution of pathogenic hospital-associated lineages uniquely adapted for survival and proliferation in the antibiotic perturbed ecology of the gastrointesti ... | 2016 | 26939857 |
| deletion of fabn in enterococcus faecalis results in unsaturated fatty acid auxotrophy and decreased release of inflammatory cytokines. | the gram-positive bacterium enterococcus faecalis can cause life-threatening infections and is resistant to several commonly used antibiotics. the type ii fatty acid pathway in bacteria is discussed as a potential target for antimicrobial therapy. however, it was shown that inhibition or deletion of its enzymes can be rescued in gram-positive bacteria by supplementation with fatty acids. here we show that by deletion of the fabn gene, which is essential for unsaturated fatty acid (ufa) synthesis ... | 2016 | 27009913 |
| thymidylate limitation potentiates cephalosporin activity toward enterococci via an exopolysaccharide-based mechanism. | multidrug resistant enterococci are major causes of nosocomial infections. prior therapy with cephalosporins increases the risk of developing an enterococcal infection due to the intrinsic resistance of enterococci to these antibiotics. while progress has been made toward understanding the genetic and biochemical mechanisms of cephalosporin resistance, available data indicate that as-yet-unidentified resistance factors must exist. here, we describe results of a screen to identify small molecules ... | 2016 | 27008338 |
| molecular diagnosis of urinary tract infections by semi-quantitative detection of uropathogens in a routine clinical hospital setting. | the objective of our study was the development of a semi-quantitative real-time pcr to detect uropathogens. two multiplex pcr reactions were designed to detect escherichia coli, klebsiella spp., enterobacter spp., citrobacter spp., proteus mirabilis, enterococcus faecalis, and pseudomonas aeruginosa. 16s based pcr was performed in parallel to detect gram-positive and gram-negative bacteria. firstly to identify non-targeted agents of infection in the same urine specimen, and secondly to quantify ... | 2016 | 26954694 |
| co-location of the oxazolidinone resistance genes optra and cfr on a multiresistance plasmid from staphylococcus sciuri. | to identify and characterize the oxazolidinone/phenicol resistance gene optra in staphylococcus isolates. | 2016 | 26953332 |
| resistance to antimicrobial agents among enterococci isolated from fecal samples of wild marine species in the southern coast of brazil. | the purpose of this study was to evaluate species distribution, antimicrobial resistance profiles, and presence of resistance genes in enterococci isolated from fecal samples of wild marine species, including seabirds (n=12), sea turtles (n=8), and mammals (n=3) found alive or dead in southern coast of brazil. enterococci were classified based on phenotypic and genotypic characteristics, tested for antibiotic susceptibility, and the presence of tet(s), tet(m), tet(l), mrsc, and erm(b) genes by p ... | 2016 | 26952995 |
| cysteamine enhances biofilm eradication efficacy of calcium hydroxide. | calcium hydroxide (ca[oh]2) is a widely used interappointment dressing, but its antibacterial property is compromised by dentin. hence, the addition of chlorhexidine (chx) with ca(oh)2 has been proposed. however, the antimicrobial efficacy of this mixture compared with ca(oh)2 alone is currently still debatable. cysteamine is a mucolytic agent used to reduce the viscosity of mucus through the disruption of proteins, which are also important components of the extracellular matrix of biofilms. the ... | 2016 | 26951960 |
| effects of dentin debris on the antimicrobial properties of sodium hypochlorite and etidronic acid. | the purpose of this study was to determine the influence of dentin powder on the concentration, ph, and antimicrobial activity of sodium hypochlorite (naocl) alone and combined with etidronic acid (hebp). | 2016 | 26951957 |
| comparative analysis of protaper and waveone systems to reduce enterococcus faecalis from root canal system in primary molars--an in vitro study. | to assess, in vitro, the ability of the protaper(™) and waveone(™) systems to reduce enterococcus faecalis contamination in primary molars. | 2016 | 26950813 |