Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
construction of recombinant pichia pastoris carrying a constitutive avbd9 gene and analysis of its activity.avian beta-defensin 9 (avbd9) is a small cationic peptide consisting of 41 amino acids that plays a crucial rule in innate immunity and acquired immunity in chickens. owing to its wide antibacterial spectrum, lack of a residue, and failure to induce bacterial drug resistance, avbd9 is expected to become a substitute for conventional antibiotics in the livestock and poultry industries. using the preferred codon of pichia pastoris, the mature avbd9 peptide was designed and synthesized, based on th ...201526370794
isolation of alkaline-tolerant bacteria from primary infected root canals.alkaline-tolerant bacteria in primary infected root canals could have enhanced survival capacity against antimicrobials commonly used in root canal treatment. the aims of this study were to isolate and characterize alkaline-tolerant bacteria before endodontic treatment (s1), after chemomechanical root canal preparation (s2), and after calcium hydroxide dressing (s3).201525638530
effectiveness of probiotic therapy for the prevention of relapse in patients with inactive ulcerative colitis.to evaluate the effectiveness of probiotic therapy for suppressing relapse in patients with inactive ulcerative colitis (uc).201526019464
early colonizing escherichia coli elicits remodeling of rat colonic epithelium shifting toward a new homeostatic state.we investigated the effects of early colonizing bacteria on the colonic epithelium. we isolated dominant bacteria, escherichia coli, enterococcus faecalis, lactobacillus intestinalis, clostridium innocuum and a novel fusobacterium spp., from the intestinal contents of conventional suckling rats and transferred them in different combinations into germfree (gf) adult rats. animals were investigated after various times up to 21 days. proliferative cell markers (ki67, proliferating cell nuclear anti ...201525012905
an alkaline phosphatase reporter for use in clostridium difficile.clostridium difficile is an anaerobic, gram-positive pathogen that causes severe gastrointestinal disease in humans and other mammals. c. difficile is notoriously difficult to work with and, until recently, few tools were available for genetic manipulation and molecular analyses. despite the recent advances in the field, there is no simple or cost-effective technique for measuring gene transcription in c. difficile other than direct transcriptional analyses (e.g., quantitative real-time pcr and ...201525576237
new role for human α-defensin 5 in the fight against hypervirulent clostridium difficile strains.clostridium difficile infection (cdi), one of the most common hospital-acquired infections, is increasing in incidence and severity with the emergence and diffusion of hypervirulent strains. cdi is precipitated by antibiotic treatment that destroys the equilibrium of the gut microbiota. human α-defensin 5 (hd5), the most abundant enteric antimicrobial peptide, is a key regulator of gut microbiota homeostasis, yet it is still unknown if c. difficile, which successfully evades killing by other hos ...201525547793
a novel method for screening of potential probiotics for high adhesion capability.to screen for potential probiotics with high adhesion capability, a chemostat model-based cultured human feces and denaturing gradient gel electrophoresis methods were applied, and the adhesion capability of the isolates was evaluated in vitro and in vivo. lactobacillus plantarum (hm218749), lactobacillus reuteri (eu547310), and enterococcus faecalis (hm218543) were isolated from the slime on the chemostat wall, as these organisms could grow better at 37°c in an anaerobic environment and could r ...201525912863
mutations associated with reduced surotomycin susceptibility in clostridium difficile and enterococcus species.clostridium difficile infection (cdi) is an urgent public health concern causing considerable clinical and economic burdens. cdi can be treated with antibiotics, but recurrence of the disease following successful treatment of the initial episode often occurs. surotomycin is a rapidly bactericidal cyclic lipopeptide antibiotic that is in clinical trials for cdi treatment and that has demonstrated superiority over vancomycin in preventing cdi relapse. surotomycin is a structural analogue of the me ...201525941217
chronic bacterial seminal vesiculitis as a potential disease entity in men with chronic prostatitis.to investigate bacterial infection in the seminal vesicles by bacteriological examination and radionuclide imaging in men with chronic prostatitis.201525721352
presence of aerobic micro-organisms and their influence on basic semen parameters in infertile men.urogenital tract infections in males are one of the significant etiological factors in infertility. in this prospective study, 72 patients with abnormal semen parameters or any other symptoms of urogenital tract infection were examined. semen analysis according to the who 2010 manual was performed together with microbial assessment: aerobic bacteria culture, chlamydia antigen test, candida culture, ureaplasma and mycoplasma-specific culture. in total, 69.4% of semen samples were positive for at ...201525209133
effects of a one year reusable contraceptive vaginal ring on vaginal microflora and the risk of vaginal infection: an open-label prospective evaluation.a contraceptive vaginal ring (cvr) containing nestorone® (nes) and ethinyl estradiol (ee) that is reusable for 1- year (13 cycles) is under development. this study assessed effects of this investigational cvr on the incidence of vaginal infections and change in vaginal microflora.201526267119
antimicrobial activity and chemometric modelling of south african propolis.this study reports on the inhibitory and bactericidal properties of 39 south african (sa) propolis samples and three propolis samples from brazil.201526189549
the efficacy of photodynamic therapy and sodium hypochlorite in root canal disinfection by a single-file instrumentation technique.the aim of this in vitro study was to evaluate the efficacy of photodynamic therapy (pdt) and sodium hypochlorite (naocl) in root canal disinfection by a single-file instrumentation technique.201526007242
antimicrobial and antifungal efficacy of spilanthes acmella as an intracanal medicament in comparison to calcium hydroxide: an in vitro study.repeated endodontic failures are due to pathogens like enterococcus faecalis and candida albicans which are resistant to the common intracanal medicaments like calcium hydroxide (ca[oh]2).201526672426
in vitro antimicrobial and anti-endotoxin action of zingiber officinale as auxiliary chemical and medicament combined to calcium hydroxide and chlorhexidine.this study was conducted in vitro to compare the effectiveness of zingiber officinale as an auxiliary chemical substance followed by placement of different intra-canal medication in removing endotoxins and cultivable micro-organisms from infected root canals.201525892281
immune response profile against persistent endodontic pathogens candida albicans and enterococcus faecalis in vitro.persistent microorganisms such as candida albicans and enterococcus faecalis might be directly related to endodontic treatment failure. the host response to these microorganisms impairs the reestablishment of intraradicular and periradicular health.201525845887
adenosine tri-phosphate (atp)-based cleaning monitoring in health care: how rapidly does environmental atp deteriorate?ensuring cleaning compliance of housekeeping staff is critical to ensure adequate application of surface disinfectants. adenosine triphosphate (atp) testing has been recommended as a way to monitor cleaning compliance; however, little is known about the stability of atp on environmental surfaces.201525794441
antimicrobial and cytotoxic activity of ferula gummosa plant essential oil compared to naocl and chx: a preliminary in vitro study.the usage of medicinal plants as natural antimicrobial agents has grown in many fields including dental medicine. the aim of this in vitro study was three-fold: (i) to determine the chemical compositions of the ferula gummosa essential oil (fgeo), (ii) to compare the antimicrobial efficacy of the oil with sodium hypochlorite (naocl) and chlorhexidine (chx), (iii) to assess the toxic behavior of fgeo in different concentrations compared to 5% naocl and 0.2% chx.201525671213
acarbose treatment affects the serum levels of inflammatory cytokines and the gut content of bifidobacteria in chinese patients with type 2 diabetes mellitus.the effects of acarbose add-on therapy on gut microbiota and inflammatory cytokines were investigated in chinese patients with type 2 diabetes mellitus (dm).201525327485
in vitro antimicrobial activity of plants used in traditional medicine in gurage and silti zones, south central ethiopia.to overcome the escalating problems associated with infectious diseases and drug resistance, discovery of new antimicrobials is crucial. the present study aimed to carry out in vitro antimicrobial analysis of 15 medicinal plant species selected according to their traditional medicinal uses in gurage and silti zones, south central ethiopia.201526283630
antibiofilm and antimicrobial activity of polyethylenimine: an interesting compound for endodontic treatment.bacteria levels of necrotic teeth are greatly reduced after endodontic treatment procedures but the presence of persisting microorganisms leads to continuous efforts to develop materials with antimicrobial properties. the purpose of the study was to determine the antimicrobial activity of polyethylenimine (pei) against common bacteria and yeasts, regarding planktonic cells and biofilm, and to clarify its antimicrobial mechanism of action through flow cytometry.201526323443
performance evaluation of a dna chip assay in the identification of major genitourinary pathogens.to prevent the recurrence of genitourinary infections and to reduce the risks of their complications, accurate and rapid diagnosis are required. stdetect® chip is a dna chip which allows for the simultaneous detection of 13 major genitourinary pathogens in a single vaginal swab or urine specimen. we evaluated the analytical performance of the stdetect® chip for detecting target pathogens that commonly cause genitourinary infections.201525533217
antimicrobial effect of zataria multiflora extract in comparison with chlorhexidine mouthwash on experimentally contaminated orthodontic elastomeric ligatures.long-term use of orthodontic appliances and fixation ligatures creates a favorable environment for the accumulation of oral normal microflora and increases the risk of enamel demineralization and periodontal disease. the aim of this study was to compare the antimicrobial effects of zataria multiflora extract and 0.2% chlorhexidine (chx) mouthwash on experimentally contaminated orthodontic elastomeric ligatures.201526005448
composition and antimicrobial activity of euphrasia rostkoviana hayne essential oil.eyebright, euphrasia rostkoviana hayne (scrophulariaceae), is a medicinal plant traditionally used in europe for the treatment of various health disorders, especially as eyewash to treat eye ailments such as conjunctivitis and blepharitis that can be associated with bacterial infections. some euphrasia species have been previously reported to contain essential oil. however, the composition and bioactivity of e. rostkoviana oil are unknown. therefore, in this study, we investigated the chemical c ...201526000025
localization of the dual oxidase bli-3 and characterization of its nadph oxidase domain during infection of caenorhabditis elegans.dual oxidases (duox) are enzymes that contain an nadph oxidase domain that produces hydrogen peroxide (h2o2) and a peroxidase domain that can utilize h2o2 to carry out a variety of reactions. the model organism caenorhabditis elegans produces the duox, bli-3, which has roles in both cuticle development and in protection against infection. in previous work, we demonstrated that while certain peroxidases were protective against the human bacterial pathogen enterococcus faecalis, the peroxidase dom ...201525909649
the in vitro antimicrobial activity of cymbopogon essential oil (lemon grass) and its interaction with silver ions.it is well known that cymbopogon (lemon grass) essential oil exhibits antimicrobial activity while the efficacy of silver ions as a disinfectant is equally well reported.201526055131
evaluation of microbial reduction in root canals instrumented with reciprocating and rotary systems.this in vitro study aimed to evaluate the efficacy of the disinfection of root canal systems carried out with reciproc(tm) and protaper universal(tm) systems using 1% sodium hypochlorite (naocl).201527688413
antibacterial, antifungal and antioxidant activity of olea africana against pathogenic yeast and nosocomial pathogens.olea africana leaves are used by bapedi people to treat different ailments. the use of these leaves is not validated, therefore the aim of this study is to validate antimicrobial properties of this plant.201526577343
utilization of blood cultures in danish hospitals: a population-based descriptive analysis.this national population-based study was conducted as part of the development of a national automated surveillance system for hospital-acquired bacteraemia and ascertains the utilization of blood cultures (bcs). a primary objective was to understand how local differences may affect interpretation of nationwide surveillance for bacteraemia. from the danish microbiology database, we retrieved all bcs taken between 2010 and 2013 and linked these to admission data from the national patient registry. ...201525658520
microbiological toxicity of tilmicosin on human colonic microflora in chemostats.to evaluate the microbiological safety of tilmicosin on human intestinal microflora, four chemostat models of healthy human colonic ecosystems were exposed to tilmicosin (0, 0.436, 4.36, and 43.6 μg/ml) for 7 days. prior to and during drug exposure, three microbiological endpoints were monitored daily including short-chain fatty acids, bacterial counts and macrolide susceptibility. colonization resistance of each community was determined by 3 successive daily challenges of salmonella typhimurium ...201526190303
[influence of the root canal instrumentation size on the disinfection of intracanal microbe by er:yag laser].to evaluate the influence of different root canal instrumentation size on disinfection of intracanal microbe of dental root canal.201527063113
antimicrobial activity of silver and gold in toothpastes: a comparative analysis.in this study, we compared the antimicrobial activity of identical toothpastes differing only in silver or gold nanoparticles against the activity of one of the common toothpastes containing a chemical active ingredient. we also compared the active concentrations of the toothpastes.201526183852
probiotic bifico cocktail ameliorates helicobacter pylori induced gastritis.to determine the protective effect of triple viable probiotics on gastritis induced by helicobacter pylori (h. pylori) and elucidate the possible mechanisms of protection.201526074694
quantitative profiling of colorectal cancer-associated bacteria reveals associations between fusobacterium spp., enterotoxigenic bacteroides fragilis (etbf) and clinicopathological features of colorectal cancer.various studies have presented clinical or in vitro evidence linking bacteria to colorectal cancer, but these bacteria have not previously been concurrently quantified by qpcr in a single cohort. we quantify these bacteria (fusobacterium spp., streptococcus gallolyticus, enterococcus faecalis, enterotoxigenic bacteroides fragilis (etbf), enteropathogenic escherichia coli (epec), and afac- or pks-positive e. coli) in paired tumour and normal tissue samples from 55 colorectal cancer patients. we f ...201525751261
enzymatic elucidation of haemocyanin from kuruma shrimp marsupenaeus japonicus and its molecular recognition mechanism towards pathogens.haemocyanin is an important non-specific immune protein present in the hemolymph of invertebrates, which have the ability to recognize the microbial pathogens and trigger the innate immune system. in this study, we isolated and purified the haemocyanin using gel filtration chromatography and investigated its microbial recognition mechanism against the invading pathogens. kuruma shrimp marsupenaeus japonicus haemocyanin showed the single band with a molecular weight of 76 kda on sds-page and its ...201525204648
[bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between july 1982 and march 2012].this is an integrated summary of the results obtained from a 3-decade multicenter study on bacteria isolated from surgical infections in japan between july 1982 and march 2012. during the 3-decade study, 11,196 strains were isolated from 4,787 patients consisting of 2,132 patients with primary infection and 2,655 patients with surgical site infection. almost half of the primary infection was peritonitis, which accounted for 42.3%-55.5%. in contrast, most of the surgical site infection was wound ...201526349116
antibacterial and antiproliferative activities of plumericin, an iridoid isolated from momordica charantia vine.plumericin, an iridoid lactone, was isolated with relatively high yield from momordica charantia vine using the supercritical fluid extraction (sfe) and the separation box (sepbox) comprising dual combination of high-performance liquid chromatography and solid phase extraction. this compound showed antibacterial activity against enterococcus faecalis and bacillus subtilis with minimum inhibitory concentration (mic) values better than cloxacillin. plumericin potently inhibited proliferation of tw ...201525945113
antibacterial mode of action of the hydroethanolic extract of leonotis nepetifolia (l.) r. br. involves bacterial membrane perturbations.leonotis nepetifolia (l) r. br., lamiaceae, a pantropical shrub, popularly known in brazil as "cordão-de-frade", "rubim", is reportedly used in brazilian ethnomedicine as well as in different countries in the treatments of ailments such as infections, inflammations, wounds, stomach disorders, among others.201526102550
antibacterial activity of the venom of heterometrus xanthopus.heterometrus xanthopus (scorpion) is one of the most venomous and ancient arthropods. its venom contains anti-microbial peptides like hadrurin, scorpine, pandinin 1, and pandinin 2 that are able to effectively kill multidrug-resistant pathogens. the present study was conducted to evaluate the anti-bacterial activity of h. xanthopus venom. six gram-positive and gram-negative bacterial strains were tested against 1/100, 1/10, and 1/1 fractions of distilled water diluted and crude venom. 1/100 and ...201523087515
chronic prostatitis: a possible cause of hematospermia.while hematospermia is mainly caused by genitourinary inflammatory disorders, very few studies have been published on prostatitis-associated hematospermia (pah) diagnosed using robust prostatitis evaluation methods. therefore, we have evaluated the incidence of pah by using systematic methods for evaluating prostatitis.201526331127
pathogen distribution and drug resistance in a burn ward: a three-year retrospective analysis of a single center in china.to investigate the spread of multiple-resistant strain in a burn ward to inform clinical administration of antibiotic drugs, burn wound treatment and decision-making for infection control. a 3-year retrospective analysis was conducted. specimens from wounds, blood, catheter, sputum, urine and stool collected from inpatients of the second affiliated hospital of zhejiang university of medicine between january 1, 2011 and december 31, 2013 were cultured and strains were identified by automatic bact ...201526770555
evaluation of regional antibiograms to monitor antimicrobial resistance in hampton roads, virginia.we studied recent antibiograms (2010 to 2011) from 12 hospitals in the hampton roads area, virginia, that refer patients to a tertiary-care facility affiliated with eastern virginia medical school. the data was compiled into a regional antibiogram, and sensitivity rates of common isolates from the tertiary-care facility (central) were compared to those of referring hospitals grouped by locale. staphylococcus aureus was the most common gram- positive and e. coli the most common gram- negative org ...201525890362
antibacterial activity of synthetic peptides derived from lactoferricin against escherichia coli atcc 25922 and enterococcus faecalis atcc 29212.peptides derived from human and bovine lactoferricin were designed, synthesized, purified, and characterized using rp-hplc and maldi-tof-ms. specific changes in the sequences were designed as (i) the incorporation of unnatural amino acids in the sequence, the (ii) reduction or (iii) elongation of the peptide chain length, and (iv) synthesis of molecules with different number of branches containing the same sequence. for each peptide, the antibacterial activity against escherichia coli atcc 25922 ...201525815317
in vitro spectrum of pexiganan activity when tested against pathogens from diabetic foot infections and with selected resistance mechanisms.pexiganan, a 22-amino-acid synthetic cationic peptide, is currently in phase 3 clinical trials as a topical antimicrobial agent for the treatment of mild infections associated with diabetic foot ulcers. bacterial isolates from the 2013 sentry antimicrobial surveillance program designated as pathogens from diabetic foot infections (dfi) and gram-negative and -positive pathogens from various infection types that harbored selected resistance mechanisms/phenotypes were tested against pexiganan in re ...201525583717
functional analysis of a bacitracin resistance determinant located on icecp1, a novel tn916-like element from a conjugative plasmid in clostridium perfringens.bacitracins are mixtures of structurally related cyclic polypeptides with antibiotic properties. they act by interfering with the biosynthesis of the bacterial cell wall. in this study, we analyzed an avian necrotic enteritis strain of clostridium perfringens that was resistant to bacitracin and produced netb toxin. we identified a bacitracin resistance locus that resembled a bacitracin resistance determinant from enterococcus faecalis. it contained the structural genes bcrabd and a putative reg ...201526282424
variability of cutaneous and nasal population levels between patients colonized and infected by multidrug-resistant bacteria in two brazilian intensive care units.to compare cutaneous and nasal population levels between patients colonized and infected by multidrug-resistant organisms in two intensive care units.201526770762
tridentate hydrazone metal complexes derived from cephalexin and 2-hydrazinopyridine: synthesis, characterization and antibacterial activity.metal(ii) coordination compounds of a tridentate hydrazone ligand (hl) derived from the condensation of cephalexin antibiotic with 2-hydrazinopyridine were synthesized. the hydrazone ligand and mononuclear [ml(oac)(h2o)] (m(ii)=mn, co, ni, cu, zn, ag) complexes were characterized by several techniques, including elemental and thermal analysis, molar conductance and magnetic susceptibility measurements, electronic, ft-ir, epr and (1)h nmr spectral studies. the cephalexin 2-pyridinylhydrazone liga ...201525677531
drug resistance and molecular epidemiology of aerobic bacteria isolated from puerperal infections in bangladesh.puerperal infection is a common complication during postnatal period in developing countries. bacterial species, drug resistance, and genetic characteristics were investigated for a total of 470 isolates from puerperal infections in bangladesh for a 2-year period (2010-2012). the most common species was escherichia coli (n=98), followed by enterococcus faecalis (n=54), staphylococcus haemolyticus (n=33), proteus mirabilis (n=32), staphylococcus aureus (n=27), klebsiella pneumoniae (n=22), and en ...201525555043
effect of treatment with an overheated dry-saturated steam vapour disinfection system on multidrug and extensively drug-resistant nosocomial pathogens and comparison with sodium hypochlorite activity.the development of portable steam generators has made disinfection of the environment more practical. this study assessed the "in vitro" ability of an overheated dry-saturated steam vapour system to kill multidrug and extensively-drug resistant nosocomial pathogens, defining the antimicrobial spectrum and the contact times compared with the activity of sodium hypochlorite.201526452549
selected compounds structurally related to acyclic sesquiterpenoids and their antibacterial and cytotoxic activity.by implementing a common and industrially used method, 30 compounds which are structurally related to geranyl acetone, nerolidol, farnesal, farnesol and farnesyl acetate were obtained. their antimicrobial activity against staphylococcus aureus, enterococcus faecalis, enterococcus faecium, escherichia coli, klebsiella pneumoniae and acinetobacter baumannii bacteria was investigated. pharmacophore models were proposed based on the obtained results and 3d qsar modelling. cytotoxic effects against m ...201526096434
global in vitro activity of tigecycline and comparator agents: tigecycline evaluation and surveillance trial 2004-2013.the tigecycline evaluation and surveillance trial (test) is a global antimicrobial susceptibility surveillance study which has been ongoing since 2004. this report examines the in vitro activity of tigecycline and comparators against clinically important pathogens collected globally between 2004 and 2013.201525958201
in vitro evaluation of antimicrobial efficacy of extracts obtained from raw and fermented wild macrofungus, lenzites quercina.in recent time, there is a major concern about antibiotic resistance displayed by some pathogenic microorganisms and this had involved a continuous search for natural antimicrobial products. the phytochemistry as well as antimicrobial activity of extracts obtained from lenzites quercina was investigated. the extracts and purified fractions were, respectively, tested against indicator organisms using agar well diffusion and disc diffusion methods. the quantity of phytochemicals found in the extra ...201526604928
immunostimulatory effects triggered by enterococcus faecalis cect7121 probiotic strain involve activation of dendritic cells and interferon-gamma production.probiotics can modulate the immune system, conferring beneficial effects on the host. understanding how these microorganisms contribute to improve the health status is still a challenge. previously, we have demonstrated that enterococcus faecalis cect7121 implants itself and persists in the murine gastrointestinal tract, and enhances and skews the profile of cytokines towards the th1 phenotype in several biological models. given the importance of dendritic cells (dcs) in the orchestration of imm ...201525978357
bacteriology of pressure ulcers in individuals with spinal cord injury: what we know and what we should know.individuals with spinal cord injury (sci) are at increased risk for the development of pressure ulcers. these chronic wounds are debilitating and contribute to prolonged hospitalization and worse medical outcome. however, the species of bacteria and the role that specific species may play in delaying the healing of chronic pressure ulcers in the sci population has not been well characterized. this study will review the literature regarding what is known currently about the bacteriology of pressu ...201525130374
antimicrobial activity of protamine against oral microorganisms.protamine is an arginine-rich polycationic protein extracted from sperm cells of vertebrates including fishes such as salmon. the purpose of this study was to investigate the suppressive effects of protamine on the growth of oral pathogens for possible usage in dental materials. minimal inhibitory concentration (mic) and minimal bactericidal concentration (mbc) were determined by the microdilution method. twelve strains of oral viridans streptococci, actinomyces naeslundii, actinomyces odontolyt ...201526699859
the impact of absorbed photons on antimicrobial photodynamic efficacy.due to increasing resistance of pathogens toward standard antimicrobial procedures, alternative approaches that are capable of inactivating pathogens are necessary in support of regular modalities. in this instance, the photodynamic inactivation of bacteria (pib) may be a promising alternative. for clinical application of pib it is essential to ensure appropriate comparison of given photosensitizer (ps)-light source systems, which is complicated by distinct absorption and emission characteristic ...201526236292
strains of enterococcus faecalis differ in their ability to coexist in biofilms with other root canal bacteria.to investigate the relationship between protease production and the ability of enterococcus faecalis strains to coexist in biofilms with other bacteria commonly recovered from infected root canals.201526172346
graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant staphylococcus aureus.methicillin-resistant staphylococcus aureus (mrsa) has been responsible for serious hospital infections worldwide. nanomaterials are an alternative to conventional antibiotic compounds, because bacteria are unlikely to develop microbial resistance against nanomaterials. in the past decade, graphene oxide (go) has emerged as a material that is often used to support and stabilize silver nanoparticles (agnps) for the preparation of novel antibacterial nanocomposites. in this work, we report the syn ...201526586946
detection of airborne bacteria in a duck production facility with two different personal air sampling devices for an exposure assessment.prevalent airborne microorganisms are not well characterized in industrial animal production buildings with respect to their quantity or quality. to investigate the work-related microbial exposure, personal bioaerosol sampling during the whole working day is recommended. therefore, bioaerosol sampling in a duck hatchery and a duck house with two personal air sampling devices, a filter-based pgp and a niosh particle size separator, was performed. subsequent, quantitative and qualitative analyses ...201525093856
the impact of carvacrol on ammonia and biogenic amine production by common foodborne pathogens.the impact of carvacrol at different levels (0.1%, 0.5%, and 1%) on ammonia (amn) and biogenic amines (bas) production by 8 common foodborne pathogens (fbps) (staphylococcus aureus, escherichia coli, klebsiella pneumoniae, enterococcus faecalis, pseudomonas aeruginosa, listeria monocytogenes, aeromonas hydrophila, and salmonella paratyphi a) was studied using a rapid high-performance liquid chromatography method. significant differences among bacteria (p < 0.05) in amn and ba production were obs ...201526580308
antimicrobial impacts of essential oils on food borne-pathogens.the antimicrobial activity of twelve essential oil (pine oil, eucalyptus, thyme, sage tea, lavender, orange, laurel, lemon, myrtle, lemon, rosemary and juniper) was tested by a disc diffusion method against food borne pathogens (escherichia coli, salmonella paratyphi a, klebsiella pneumoniae, yersinia enterocolitica, pseudomonas aeruginosa, aeromonas hydrophila, campylobacter jejuni, enterococcus faecalis, staphylococcus aureus). the major components in essential oils were monoterpenes hydrocarb ...201526072990
combined effect of a mixture of tetracycline, acid, and detergent, and nisin against enterococcus faecalis and actinomyces viscosus biofilms.to evaluate the combined effect of a mixture of tetracycline, acid, and detergent (mtad) and nisin against enterococcus faecalis (e. faecalis) and actinomyces viscosus (a. viscosus) biofilms.201525719587
aerobic bacterial microbiota isolated from the cloaca of the european pond turtle (emys orbicularis) in poland.we conducted a comparative analysis of the aerobic cloacal bacteria of european pond turtles (emys orbicularis) living in their natural environment and juvenile turtles reared under controlled conditions in a breeding center. we included 130 turtles in the study. the aerobic bacteria isolated from the cloaca of the juvenile turtles were less diverse and more prevalent than the bacteria isolated from free-living adults. we isolated 17 bacterial species from juvenile captive turtles, among which t ...201525380369
comparison of riboflavin and toluidine blue o as photosensitizers for photoactivated disinfection on endodontic and periodontal pathogens in vitro.photoactivated disinfection has a strong local antimicrobial effect. in the field of dentistry it is an emerging adjunct to mechanical debridement during endodontic and periodontal treatment. in the present study, we investigate the effect of photoactivated disinfection using riboflavin as a photosensitizer and blue led light for activation, and compare it to photoactivated disinfection with the widely used combination of toluidine blue o and red light. riboflavin is highly biocompatible and can ...201526469348
enterococcus faecalis lipoteichoic acid suppresses aggregatibacter actinomycetemcomitans lipopolysaccharide-induced il-8 expression in human periodontal ligament cells.periodontitis is caused by multi-bacterial infection and aggregatibacter actinomycetemcomitans and enterococcus faecalis are closely associated with inflammatory periodontal diseases. although lipopolysaccharide (lps) of a. actinomycetemcomitans (aa.lps) and lipoteichoic acid of e. faecalis (ef.lta) are considered to be major virulence factors evoking inflammatory responses, their combinatorial effect on the induction of chemokines has not been investigated. in this study, we investigated the in ...201525840438
microleakage of bacteria in different implant-abutment assemblies: an in vitro study.the aim of the present in vitro study was to evaluate the leakage observed for 2 different microbial species at the level of the implant-abutment (i-a) interface, and the marginal fit and size of microgap at the i-a interface in 2 different implant connections.201525589158
antimicrobial activity of different filling pastes for deciduous tooth treatment.guedes-pinto paste is the filling material most employed in brazil for endodontic treatment of deciduous teeth; however, the rifocort® ointment has been removed. thus, the aim of this study was to investigate the antimicrobial potential of filling pastes, by proposing three new pharmacological associations to replace rifocort® ointment with drugs of already established antimicrobial power: nebacetin® ointment, 2% chlorhexidine gluconate gel, and maxitrol® ointment. a paste composed of iodoform, ...201525466327
physicochemical and biological properties of oxovanadium(iv), cobalt(ii) and nickel(ii) complexes with oxydiacetate anions.the potentiometric and conductometric titration methods have been used to characterize the stability of series of vo(iv)-, co(ii)- and ni(ii)-oxydiacetato complexes in dmso-water solutions containing 0-50 % (v/v) dmso. the influence of dmso as a co-solvent on the stability of the complexes as well as the oxydiacetic acid was evaluated. furthermore, the reactivity of the complexes towards superoxide free radicals was assessed by employing the nitro blue tetrazolium (nbt) assay. the biological pro ...201525488702
study on in vivo effects of bacterial histidine kinase inhibitor, waldiomycin, in bacillus subtilis and staphylococcus aureus.two-component signal transduction systems (tcss) represent one of the primary means by which bacteria sense and respond to changes in their environment, both intra- and extracellular. the highly conserved walk (histidine kinase)/walr (response regulator) tcs is essential for cell wall metabolism of low g+c gram-positive bacteria and acts as a master regulatory system in controlling and coordinating cell wall metabolism with cell division. waldiomycin, a walk inhibitor, has been discovered by scr ...201526582287
probiotic potential of lactobacillus strains isolated from sorghum-based traditional fermented food.sorghum-based traditional fermented food was screened for potential probiotic lactic acid bacteria. the isolates were identified by biochemical, physiological and genetic methods. species identification was done by 16s rrna sequence analysis. the functional probiotic potential of the two lactobacillus species viz., lactobacillus plantarum (lact. plantarum) and lactobacillus pentosus (lact. pentosus) was assessed by different standard parameters. the strains were tolerant to ph 2 for 1 h and resi ...201525666113
optimizing supercritical carbon dioxide in the inactivation of bacteria in clinical solid waste by using response surface methodology.clinical solid waste (csw) poses a challenge to health care facilities because of the presence of pathogenic microorganisms, leading to concerns in the effective sterilization of the csw for safe handling and elimination of infectious disease transmission. in the present study, supercritical carbon dioxide (sc-co2) was applied to inactivate gram-positive staphylococcus aureus, enterococcus faecalis, bacillus subtilis, and gram-negative escherichia coli in csw. the effects of sc-co2 sterilization ...201525636860
oxidative stress and antibacterial properties of a graphene oxide-cystamine nanohybrid.oxidative stress can damage proteins, dna, and lipids, and is involved in the progression of many diseases. damage to infected cells caused by oxidative stress is related to increased levels of reactive oxygen species, including hydrogen peroxide. during oxidative stress, hydrogen peroxide levels are often increased and catalase level decreased inside cells. this can lead to the death of skin and other cells. hydrophobic low molecular weight compounds are useful in treating hemorrhagic condition ...201525609960
[antimicrobial activity of actinomycetale isolated from the lagoon in algeria].in the aim of the study of the taxonomy and the antimicrobial activity, a strain of actinomycete sm2/2gf which was isolated from sediment of the lagoon el-mellah which is situated in the city of el-kala in the northeast of algeria, was tested against diverse pathogenic microorganisms and against a gram-negative bacterium pseudomonas alcaliphila which was isolated from water of the lagoon el-mellah. the phenotypic and the molecular characteristics show that the isolate sm2/2gf belongs to the kind ...201525847739
nanostructured sensor based on carbon nanotubes and clavanin a for bacterial detection.unusual methods for specific detection of pathogenic bacteria are becoming key points for control and identification of problems related to health and (bio)safety. in this context, this work aims to propose a new approach for the development of nanostructured biosensors based on carbon nanotubes (cnts) and antimicrobial peptides for bacterial detection. firstly, the antimicrobial peptide clavanin a (clava) was chemically immobilized on cnts and surface-immobilized clava was used to detect klebsi ...201525847459
auranofin exerts broad-spectrum bactericidal activities by targeting thiol-redox homeostasis.infections caused by antibiotic-resistant bacteria are a rising public health threat and make the identification of new antibiotics a priority. from a cell-based screen for bactericidal compounds against mycobacterium tuberculosis under nutrient-deprivation conditions we identified auranofin, an orally bioavailable fda-approved antirheumatic drug, as having potent bactericidal activities against both replicating and nonreplicating m. tuberculosis. we also found that auranofin is active against o ...201525831516
antimicrobial and antioxidant properties of the crude peptide extracts of galatea paradoxa and patella rustica.this study evaluated the antimicrobial and antioxidant activities of crude peptide extracted from galatea paradoxa (g. paradoxa) and patella rustica (p. rustica). the extracts were tested against eight strains of bacteria (escherichia coli, staphylococcus aureus, bacillus subtilis, salmonella typhi, enterococcus feacalis, klebseilla pneumoniae, streptococcus pneumoniae, pseudomonas aeruginosa) and one strain of fungi (candida albicans) using agar well diffusion and broth dilution assays. the ext ...201526405620
graphene oxide-modified zno particles: synthesis, characterization, and antibacterial properties.nanosized zno particles with diameters of 15 nm were prepared with a solution precipitation method at low cost and high yield. the synthesis of the particles was functionalized by the organic solvent dimethylformamide, and the particles were covalently bonded to the surface of graphene oxide. the morphology of the graphene oxide sheets and zno particles was confirmed with field emission scanning electron microscopy and biological atomic force microscopy. fourier transform infrared spectroscopy a ...201526347126
antimicrobial action of sodium hypochlorite and castor oil solutions for denture cleaning - in vitro evaluation.the objective of this in vitro study was to evaluate the antimicrobial action of sodium hypochlorite (0.25% and 0.50%) and 10% castor oil solutions against specific microorganisms, by counting colony forming units (cfu) of clinically important bacteria and candida species. acrylic resin specimens (n = 320; lucitone 550) were obtained from square metal matrices (10 x 10 x 2 mm), sterilized by microwave (650w, for 6 minutes) and contaminated by staphylococcus aureus, pseudomonas aeruginosa, candid ...201526313346
synthesis, antibacterial and qsar evaluation of 5-oxo and 5-thio derivatives of 1,4-disubstituted tetrazoles.a series of 1,4-disubstituted tetrazol-5-ones 3a, 5, 7, 12, 13 and 1,4-disubstituted tetrazol-5-thiones 3b, 9, 10 was synthesized and fully characterized by ir, ms, (1)h nmr and (13)c nmr. the series was evaluated for in vitro antibacterial activity against four gram negative (escherichia coli, proteus mirabilis, klebsiella pneumonia, and pseudomonas aeruginosa) and three gram positive (staphylococcus aureus, enterococcus faecalis, and bacillus subtilis) bacteria. the zone of inhibition was meas ...201526243367
biological activities of morus celtidifolia leaf extracts.the aims of this research were to examine the antibacterial, cytotoxic and antiradical/antioxidant activities of the organic extracts obtained from the leaves of the medicinal plant morus celtidifolia (family: moraceae). to evaluate its antimicrobial properties, m. celtidifolia was tested against the bacteria of medical importance: bacillus subtilis, staphyloccocus aureus, enterococcus faecalis, escherichia coli, enterobacter cloacae and enterobacter aerogenes. cytotoxic activity was assessed by ...201526142508
the length of a lantibiotic hinge region has profound influence on antimicrobial activity and host specificity.lantibiotics are ribosomally synthesized (methyl)lanthionine containing peptides which can efficiently inhibit the growth of gram-positive bacteria. as lantibiotics kill bacteria efficiently and resistance to them is difficult to be obtained, they have the potential to be used in many applications, e.g., in pharmaceutical industry or food industry. nisin can inhibit the growth of gram-positive bacteria by binding to lipid ii and by making pores in their membrane. the c-terminal part of nisin is ...201525688235
biological activities of xylooligosaccharides generated from garlic straw xylan by purified xylanase from bacillus mojavensis ueb-fk.a newly isolated bacterium strain named ueb-fk was selected from tunisian sahara, exhibiting the highest clear zone on agar plates containing oat spelt xylan by staining with congo red. on the basis of 16s rdna sequence analysis, this strain was identified as bacillus mojavensis. this strain produced extracellular xylanase. xylanase from the strain was purified to homogeneity and had an apparent molecular weight of 14 kda. the k m and v max values of the purified xylanase on oat spelt xylan were ...201525351626
antibacterial effects and action modes of asiatic acid.in this study, the antibacterial effects and action modes of asiatic acid against the foodborne bacterial pathogens escherichia coli o157:h7, salmonella typhimurium dt104, pseudomonas aeruginosa, listeria monocytogenes, staphylococcus aureus, enterococcus faecalis, and bacillus cereus were examined. minimal inhibitory concentrations (mics) of asiatic acid against these bacteria were in the range of 20-40 μg/ml. minimum bactericidal concentrations of asiatic acid were in the range of 32-52 μg/ml. ...201526280399
antimicrobial evaluation of plants used for the treatment of diarrhoea in a rural community in northern maputaland, kwazulu-natal, south africa.zulu people living in the rural area of maputaland (kwazulu-natal, south africa) rely heavily on medicinal plants for the treatment of diarrhoea. abundant availability of medicinal plants in the study area offers low cost health care, but scientific validation is needed in order to lend credibility to the traditional use against many ailments including diarrhoeal infections. with this in mind a study was designed to test the in vitro antimicrobial efficacy of 23 plant species which are used for ...201525881066
pharmacological basis for the medicinal use of linum usitatissimum (flaxseed) in infectious and non-infectious diarrhea.linum usitatissimum, commonly known as flaxseed has traditionally been used for the management of diarrhea and gastrointestinal infections. this study was planned to assess pharmacological basis for the medicinal use of flaxseed in infectious and non-infectious diarrhea.201525433250
use of enterococcus faecalis and bacillus atrophaeus as surrogates to establish and maintain laboratory proficiency for concentration of water samples using ultrafiltration.the u.s. environmental protection agency's (epa) water laboratory alliance (wla) currently uses ultrafiltration (uf) for concentration of biosafety level 3 (bsl-3) agents from large volumes (up to 100-l) of drinking water prior to analysis. most uf procedures require comprehensive training and practice to achieve and maintain proficiency. as a result, there was a critical need to develop quality control (qc) criteria. because select agents are difficult to work with and pose a significant safety ...201526306940
surrogate testing suggests that chlorine dioxide gas exposure would not inactivate ebola virus contained in environmental blood contamination.the ability to decontaminate a room potentially containing the ebola virus is important to healthcare facilities in the united states. ebola virus remains viable in body fluids, a room that has housed a patient with ebola virus disease must have all surfaces manually wiped with an approved disinfectant, which increases occupational exposure risk. this study evaluated the efficacy of gaseous chlorine dioxide inactivation of bacterial organisms in blood as ebola virus surrogates and as the organis ...201525955403
combination of microbiological culture and multiplex pcr increases the range of vaginal microorganisms identified in cervical cancer patients at high risk for bacterial vaginosis and vaginitis.bacterial vaginosis (bv) and vaginitis in cervical cancer patients might becaused by mixed aerobic, anaerobic, and atypical bacteria. since genital tract infections can be complicated, early and accurate identification of causal pathogens is vital.201526117968
bivalent transition metal complexes of ono donor hydrazone ligand: synthesis, structural characterization and antimicrobial activity.mononuclear transition metal complexes of mn(ii), co(ii), ni(ii), cu(ii), zn(ii) and cd(ii) with a new hydrazone ligand derived from pyrazine-2-carbohydrazide and 2-hydroxyacetophenone have been synthesized. the isolated complexes were characterized by elemental analysis, spectral and analytical methods including elemental analyses, ir, diffuse reflectance, (1)h-nmr, mass spectra, molar conductance, magnetic moment, esr, xrd, tg and sem analysis. from the elemental analyses data, the stoichiomet ...201526163785
chemical composition and antibacterial activities of seven eucalyptus species essential oils leaves.in this paper, we have studied the essential oils chemical composition of the leaves of seven eucalyptus species developed in tunisia. eucalyptus leaves were picked from trees growing in different arboretums in tunisia. choucha and mrifeg arboretums located in sedjnene, region of bizerte (choucha: e. maideni, e. astrengens et e. cinerea; mrifeg : e. leucoxylon), korbous arboretums located in the region of nabeul, north east tunisia with sub-humid bioclimate, (e. lehmani), souiniet-ain drahem arb ...201525654423
integration of non-oral bacteria into in vitro oral biofilms.biofilms are polymicrobial communities that grow on surfaces in nature. oral bacteria can spontaneously form biofilms on the surface of teeth, which may compromise the health of the teeth, or their surrounding (periodontal) tissues. while the oral bacteria exhibit high tropism for their specialized ecological niche, it is not clear if bacteria that are not part of the normal oral microbiota can efficiently colonize and grow within oral biofilms. by using an in vitro "supragingival" biofilm model ...201525483866
the all-alpha domains of coupling proteins from the agrobacterium tumefaciens virb/vird4 and enterococcus faecalis pcf10-encoded type iv secretion systems confer specificity to binding of cognate dna substrates.bacterial type iv coupling proteins (t4cps) bind and mediate the delivery of dna substrates through associated type iv secretion systems (t4sss). t4cps consist of a transmembrane domain, a conserved nucleotide-binding domain (nbd), and a sequence-variable helical bundle called the all-alpha domain (aad). in the t4cp structural prototype, plasmid r388-encoded trwb, the nbd assembles as a homohexamer resembling reca and dna ring helicases, and the aad, which sits at the channel entrance of the hom ...201525939830
microbiological stability of canned tuna produced in italy and in non-european countries.the results of a study on the microbiological stability of canned tuna produced by italian companies and similar canned products manufactured in countries outside europe are reported herein. the study involved 38 samples of canned tuna of various brands, of which 14 were produced by companies outside europe and 24 by italian companies. qualitative and quantitative microbiological tests were conducted for the following parameters: bacterial colony counts at 30°c, total coliforms, total enterobact ...201527800383
antimicrobial and cytotoxic effects of phosphoric acid solution compared to other root canal irrigants.phosphoric acid has been suggested as an irrigant due to its effectiveness in removing the smear layer.201526018307
[bloodstream infections in southwestern china: 2012 whire union report on bacterial susceptibility to antibiotics].to test the susceptibility of blood culture isolates to antibiotics in a sample of hospitals in southwestern china in 2012.201525993745
ammonia sanitization of blackwater for safe use as fertilizer.source-separated blackwater from low-flush toilets contains plant-available nutrients and can be used as a fertilizer. the aim of the study was to evaluate the impact on pathogen inactivation when treating blackwater with urea and/or lime. blackwater was spiked with salmonella typhimurium, escherichia coli o157, enterococcus faecalis, and ascaris suum eggs, and treated with urea and/or lime in concentrations up to 0.1% w/w. the bottles were kept in a storage facility (manure slurry tank) for 102 ...201525768229
epidemiology and antibiotic susceptibility of aerococci in urinary cultures.in this study, we present population-based data regarding the prevalence of aerococci in clinical urinary samples. during a 3-month period, all aerococcal isolates from urinary samples from 2 clinical microbiology laboratories were collected. we identified 64 aerococcus urinae isolates and 40 aerococcus sanguinicola isolates, which correlates with an incidence of 33 cases of aerococcal bacteriuria per 100,000 inhabitants per year. the median age was 83years for all patients with aerococcal bacte ...201525497460
bactericidal activity and mechanism of photoirradiated polyphenols against gram-positive and -negative bacteria.the bactericidal effect of various types of photoirradiated polyphenols against gram-positive and -negative bacteria was evaluated in relation to the mode of action. gram-positive bacteria (enterococcus faecalis, staphylococcus aureus, and streptococcus mutans) and gram-negative bacteria (aggregatibacter actinomycetemcomitans, escherichia coli, and pseudomonas aeruginosa) suspended in a 1 mg/ml polyphenol aqueous solution (caffeic acid, gallic acid, chlorogenic acid, epigallocatechin, epigalloca ...201525660393
antimicrobial activity and chemical composition of the essential oils of portuguese foeniculum vulgare fruits.the aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils obtained by hydrodistillation from fruits of six fennel accessions collected from wild populations occurring in the centre and south of portugal. composition of essential oils was established by gas chromatography-flame ionization detector (gc-fid) and gas chromatography-mass spectrometry (gc-ms) analysis. the obtained yields of the essential oils were found to vary greatly in the range ...201525973507
[preliminary analyses on bacterial diversity and resistance in infection-related skin disorders].to explore the bacterial diversity and resistance in infection-related skin disorders.201526704163
Displaying items 13101 - 13200 of 14299