Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| [hyaluronic acid production by streptococcus iniae and its application in rabbit skin's regeneration]. | hyaluronic acid (ha) is an important biomaterial as the extracellular matrix in human body. we produced ha by fermentation of streptococcus iniae (strep.). production of ha by strep. was evaluated and further improved by strain mutation by ultraviolet. one strain with higher ha yield and lower content of protein was obtained. its ha yield increased from (82.3±3.3) mg/l to (120±10.6) mg/l, and protein decreased from (0.178±0.011) mg/l to (0.032±0.017) mg/l. the molecular weight (mw) of ha yield f ... | 2016 | 29022311 |
| molecular and functional characterization of caspase-8 from the big-belly seahorse (hippocampus abdominalis). | apoptosis is a physiological process that can also participate in host immune defense mechanisms, including tumor growth suppression along with homeostasis and maturation of immune cells. caspases are known to be involved in cellular apoptotic signaling; among them, caspase-8 plays an important role in the initiation phase of the apoptotic death cascade. in the current study, we molecularly characterized a caspase-8 homolog (designated as hacasp-8) from hippocampus abdominalis. the hacasp-8 gene ... | 2016 | 27732898 |
| effects of dietary spirulina platensis on growth performance, humoral and mucosal immune responses and disease resistance in juvenile great sturgeon (huso huso linnaeus, 1754). | dietary supplementation of spirulina platensis at different levels (0% control, 2.5%, 5% and 10%) was evaluated to find out the effects on growth performance, digestive enzyme activities, humoral and skin innate immune responses and disease resistance in the great sturgeon (huso huso). after 8 weeks of experimental trial, growth parameters, intestinal lactic acid bacteria count, protease and lipase activities were significantly high in 10% s. platensis fed group (p < 0.05). similarly, in this gr ... | 2016 | 27506276 |
| pharmacokinetics, pharmacokinetic-pharmacodynamic relationship, and withdrawal period of amoxicillin sodium in olive flounder (paralichthys olivaceus). | 1. the pharmacokinetics (pk) and withdrawal period of amoxicillin sodium in olive flounder and its activity against pathogenic bacteria of olive flounder were investigated. 2. intramuscular administration (12.5 or 125 mg/kg, n = 160) and hplc analysis of sera were used. 3. rapid absorption (tmax 2.6 and 2.2 h), prolonged action (terminal half-life, 15.52 and 10.42 h; mrt, 18.79 and 14.44 h), and dose-proportional exposure (auc0-∞, 273.69 and 2755.37 h. μg/ml) were observed after 12.5 and 125 mg/ ... | 2016 | 26407038 |
| a prebiotic role of ecklonia cava improves the mortality of edwardsiella tarda-infected zebrafish models via regulating the growth of lactic acid bacteria and pathogen bacteria. | in this study, the beneficial prebiotic roles of ecklonia cava (e. cava, ec) were evaluated on the growth of lactic acid bacteria (lab) and pathogen bacteria and the mortality of pathogen-bacteria infected zebrafish model. the result showed that the original e. cava (ec) led to the highest growth effects on three labs (lactobacillus brevis, l. brevis; lactobacillus pentosus, l. pentosus; lactobacillus plantarum; l. plantarum) and it was dose-dependent manners. also, ec, its celluclast enzymatic ... | 2016 | 27192145 |
| thioredoxin domain-containing protein 12 from oplegnathus fasciatus: molecular characterization, expression against immune stimuli, and biological activities related to oxidative stress. | thioredoxin domain-containing protein 12 (txndc12) is a small, disulfide-containing protein that belongs to the thioredoxin (txn) superfamily. in the present study, we identified and characterized a txndc12-like gene, designated oftxndc12, from rock bream, oplegnathus fasciatus. oftxndc12 consists of seven exons interrupted by six introns. comparative genomic structural analysis revealed that the txndc12 of vertebrates is a structurally conserved gene. the coding sequence of oftxndc12 comprises ... | 2016 | 27026037 |
| isolation and characterization of bacteria with antibacterial properties from nile tilapia (oreochromis niloticus). | one hundred and twenty bacterial isolates were obtained from the intestinal mucus of nile tilapia (oreochromis niloticus) and screened for antagonistic activity and adherence abilities. based on in vitro antagonism against two pathogens (streptococcus iniae and edwardsiella piscicida), five isolates were selected and identified by 16s rrna gene sequence analysis. all antagonistic isolates were affiliated to the genus bacillus, which showed inhibitory activity against s. iniae. only the isolate b ... | 2016 | 27033910 |
| characterization of a 1-cysteine peroxiredoxin from big-belly seahorse (hippocampus abdominalis); insights into host antioxidant defense, molecular profiling and its expressional response to septic conditions. | 1-cysteine peroxiredoxin (prx6) is an antioxidant enzyme that protects cells by detoxifying multiple peroxide species. this study aimed to describe molecular features, functional assessments and potential immune responses of prx6 identified from the big-belly seahorse, hippocampus abdominalis (haprx6). the complete orf (666 bp) of haprx6 encodes a polypeptide (24 kda) of 222 amino acids, and harbors a prominent peroxiredoxin super-family domain, a peroxidatic catalytic center, and a peroxidatic ... | 2016 | 27542613 |
| antimicrobial activity of essential oil of eucalyptus globulus against fish pathogenic bacteria. | the antibacterial activities of the essential oil of eucalyptus globulus (eoeg) was determined against 7 fish pathogenic bacteria (edwardsiella tarda, streptococcus iniae, s. parauberis, lactococcus garviae, vibrio harveyi, v. ichthyoenteri and photobacterium damselae) obtained from farmed olive flounder. the inhibitory activity was evaluated by three methods: disc diffusion method, minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc). according to the disc diffusi ... | 2016 | 27382376 |
| molecular cloning and expression analysis of a new lily-type lectin in the rock bream, oplegnathus fasciatus. | a new lily-type lectin rbltl was identified from rock bream (oplegnathus fasciatus) and its expression analysed. in this study, a new lily-type lectin gene (rbltl) was cloned from rock bream using expressed sequence tag (est) analysis. the full-length rbltl cdna was encoding a 117-amino acid protein. the deduced amino acid sequence of rbltl contained all of the conserved features crucial for its fundamental structure, including b-lectin domain and three d-mannose binding sites. rbltl mrna was pr ... | 2016 | 27345170 |
| a prebiotic effect of ecklonia cava on the growth and mortality of olive flounder infected with pathogenic bacteria. | olive flounder (paralichthys olivaceus), also known as the japanese flounder in japan, is one of the most important commercial marine finfish species cultured in korea and japan. the purpose of this study was to evaluate how a species of brown algae (ecklonia cava, e. cava) affects the growth rate of olive flounder and its immune response to pathogenic bacteria. first, the experimental fish were divided into four groups: the control group was fed the diet containing only 1.0% lactobacillus plant ... | 2016 | 26921543 |
| antimicrobial response of galectin-1 from rock bream oplegnathus fasciatus: molecular, transcriptional, and biological characterization. | in this study, we describe the identification and characterization of a proto type galectin, galectin-1, from rock bream oplegnathus fasciatus (ofgal-1). galectins are evolutionarily conserved carbohydrate binding lectins that show a wide range of functions related to development and immune physiology. they have been identified as pattern recognition receptors of innate immune system that recognize a broad range of microbes. ofgal-1 cdna comprised of 993 bp with an open reading frame of 408 bp t ... | 2016 | 26792759 |
| first comparative characterization of three distinct ferritin subunits from a teleost: evidence for immune-responsive mrna expression and iron depriving activity of seahorse (hippocampus abdominalis) ferritins. | ferritins play an indispensable role in iron homeostasis through their iron-withholding function in living beings. in the current study, cdna sequences of three distinct ferritin subunits, including a ferritin h, a ferritin m, and a ferritin l, were identified from big belly seahorse, hippocampus abdominalis, and molecularly characterized. complete coding sequences (cds) of seahorse ferritin h (haferh), ferritin m (haferm), and ferritin l (haferl) subunits were comprised of 531, 528, and 522 bas ... | 2016 | 26747640 |
| coagulation factor ii from rock bream (oplegnathus fasciatus): first report on the molecular biological function and expression analysis in the teleost. | the rapid haemostasis of fish prevents bleeding or infection that could be caused by physical properties of the aquatic environment. additionally, the innate immune system is the first line of defence against infection and is responsible for the recognition of pathogen-associated molecular patterns, which are important for the activation of acquired immune responses. coagulation factor ii (cfii) is an important factor in the coagulation system and is involved in recognition and interaction with ... | 2016 | 26626585 |
| a galectin related protein from oplegnathus fasciatus: genomic, molecular, transcriptional features and biological responses against microbial pathogens. | galectins, a family of β-galactoside-binding lectins, are pattern recognition receptors that recognize pathogen-associated molecular patterns and are subsequently involved in the opsonization, phagocytosis, complement activation, and killing of microbes. here, we report a novel galectin related protein (grp) identified from rock bream (oplegnathus fasciatus), designated ofgal like b. the cdna of ofgal like b is 517 bp with an open reading frame (orf) of 438 bp, encoding 145 amino acids, with a s ... | 2016 | 26615008 |
| molecular, genomic, and expressional delineation of a piscidin from rock bream (oplegnathus fasciatus) with evidence for the potent antimicrobial activities of of-pis1 peptide. | the piscidin family comprises a group of antimicrobial peptides (amps) that are vital components of teleost innate immunity. piscidins protect the host from pathogens, through multifaceted roles as immunomodulators and anti-infective peptides. the present study reports the identification, and characterization of a putative piscidin homolog, of-pis1, from rock bream (oplegnathus fasciatus). a combined genomic and transcriptomic approach revealed that the of-pis1 gene comprises 1396 nucleotides (n ... | 2016 | 26549174 |
| first description of programmed cell death10 (pdcd10) in rock bream (oplegnathus fasciatus): potential relations to the regulation of apoptosis by several pathogens. | in this study, we isolated and characterized programmed cell death10 (pdcd10), which is known to be related to apoptosis, from rock bream (oplegnathus fasciatus). the full-length rock bream pdcd10 (rbpdcd10) cdna (1459 bp) contains an open reading frame of 633 bp that encodes 210 amino acids. furthermore, multiple alignments revealed that the six of the α-helix bundles were well conserved among the other pdcd10 sequences tested. rbpdcd10 was significantly expressed in the liver, rbc (red blood c ... | 2016 | 26472617 |
| complement factor d homolog involved in the alternative complement pathway of rock bream (oplegnathus fasciatus): molecular and functional characterization and immune responsive mrna expression analysis. | the complement system serves conventional role in the innate defense against common invading pathogens. complement factor d (cfd) is vital to alternative complement pathway activation in cleaving complement factor b. this catalytic reaction forms the alternative c3 convertase that is crucial for complement-mediated pathogenesis. in this study, rock bream (oplegnathus fasciatus) cfd (ofcfd) was characterized and ofcfd mrna expression was investigated. ofcfd encodes 277 amino acids (aa) for a 30-k ... | 2016 | 27311435 |
| host-derived probiotics enterococcus casseliflavus improves resistance against streptococcus iniae infection in rainbow trout (oncorhynchus mykiss) via immunomodulation. | the present study evaluated the benefits of dietary administration of host-derived candidate probiotics enterococcus casseliflavus in juvenile rainbow trout oncorhynchus mykiss. experimental diets were prepared by incorporating the microorganisms in the basal feed at 3 inclusion levels (i.e. 10(7) cfu g(-1) of feed [t1], 10(8) cfu g(-1) of feed [t2], 10(9) cfu g(-1) of feed [t3]). the probiotic feeds were administered for 8 weeks, with a group fed with the basal diet serving as control. the effe ... | 2016 | 26997202 |
| protective efficacy of streptococcus iniae derived enolase against streptococcal infection in a zebrafish model. | enolase (eno) is one of the surface-exposed proteins of streptococcus iniae, which previously had been identified as a plasminogen-binding protein. in this study, eno was evaluated to induce cross-protective immunity against s. iniae and streptococcus parauberis which are major pathogens causing streptococcosis in fish. immunoblot analysis shows that s. iniae recombinant eno (reno) produced in escherichia coli was cross-reactive with antisera against s. iniae, and s. parauberis serotype i and ii ... | 2016 | 26872628 |
| development of real-time pcr for detection and quantitation of streptococcus parauberis. | streptococcus parauberis is an increasing threat to aquaculture of olive flounder, paralichthys olivaceus temminck & schlegel, in south korea. we developed a real-time polymerase chain reaction (pcr) method using the taqman probe assay to detect and quantify s. parauberis by targeting the gyrb gene sequences, which are effective for molecular analysis of the genus streptococcus. our real-time pcr assay is capable of detecting 10 fg of genomic dna per reaction. the intra- and interassay coefficie ... | 2016 | 25345976 |
| antimicrobial and antileishmanial activities of diterpenoids isolated from the roots of salvia deserta. | four diterpenes with biological activity were isolated from salvia deserta roots. taxodione was considered leishmanicidal with an ic50 value of 1.46 µm (0.46 mg/l) against leishmania donovani and also exhibited antifungal and antimicrobial activities. ferruginol displayed the greatest activity [24-h ic50 of 4.5 µm (1.29 mg/l)] against the fish pathogenic bacteria streptococcus iniae. the crude extract fraction that contained the isolated compounds 7-o-acetylhorminone and horminone showed stronge ... | 2016 | 26308356 |
| pellet feed adsorbed with the recombinant lactococcus lactis bfe920 expressing sima antigen induced strong recall vaccine effects against streptococcus iniae infection in olive flounder (paralichthys olivaceus). | the aim of this study was to develop a fish feed vaccine that provides effective disease prevention and convenient application. a lactic acid bacterium (lab), lactococcus lactis bfe920, was modified to express the sima antigen, a membrane protein of streptococcus iniae. the antigen was engineered to be expressed under the nisin promoter, which is induced by nisin produced naturally by the host lab. various sizes (40 ± 3.5 g, 80 ± 2.1 g, and 221 ± 2.4 g) of olive flounder (paralichthys olivaceus) ... | 2016 | 27302864 |
| glyceradehyde-3-phosphate dehydrogenase as a suitable vaccine candidate for protection against bacterial and parasitic diseases. | the enzyme glyceraldehyde-3-p-dehydrogenase (gapdh) has been identified as having other properties in addition to its key role in glycolysis. the ability of gapdh to bind to numerous extracellular matrices, modulation of host-immune responses, a role in virulence and surface location has prompted numerous investigators to postulate that gapdh may be a good vaccine candidate for protection against numerous pathogens. although immune responses against gapdh have been described for many microorgani ... | 2016 | 26686572 |
| thermal preference predicts animal personality in nile tilapia oreochromis niloticus. | environmental temperature gradients provide habitat structure in which fish orientate and individual thermal choice may reflect an essential integrated response to the environment. the use of subtle thermal gradients likely impacts upon specific physiological and behavioural processes reflected as a suite of traits described by animal personality. in this study, we examine the relationship between thermal choice, animal personality and the impact of infection upon this interaction. we predicted ... | 2016 | 27219014 |
| interleukin-8 holds promise to serve as a molecular adjuvant in dna vaccination model against streptococcus iniae infection in fish. | dna vaccines had been widely used in animal models against various viral infections, while it was not so convincing for many infectious diseases especially bacterial disease in aquaculture. interleukin-8(il-8) as one of the cxc chemokines, its immunological role and adjuvant potential which had been proved in mammals were rarely reported in fish species. in this study, recombination plasmid pcdna3.1/il-8(pcil-8) was conducted and the capacity of il-8 as molecular adjuvant was explored from sever ... | 2016 | 27911873 |
| the expression signatures of neuronal nitric oxide synthase (nos1) in turbot (scophthalmus maximus l.) mucosal surfaces against bacterial challenge. | the mucosal surfaces constitute the first immune barrier of host defense and also serve as the dynamic interfaces that simultaneously mediate a diverse array of critical physiological processes. it has been long hypothesized that observed difference of disease resistance among different fish strains and species are strongly correlated to the activities of the immune actors in mucosal surfaces. particularly, neuronal nos (nnos or nos1) is a constitutively expressed gene that catalyzes the oxidati ... | 2016 | 27825948 |
| identification and expression analysis of toll-like receptor genes (tlr8 and tlr9) in mucosal tissues of turbot (scophthalmus maximus l.) following bacterial challenge. | mucosal immune system is one of the most important components in the innate immunity and constitutes the front line of host defense against infection, especially for teleost, which are living in the pathogen-rich aquatic environment. the pathogen recognition receptors (prrs), which can recognize the conserved pathogen-associated molecular patterns (pamps) of bacteria, are considered as one of the most important component for pathogen recognition and immune signaling pathways activation in mucosa ... | 2016 | 27633670 |
| cpg odn mimicking cpg rich region of myxosporean myxobolus supamattayai stimulates innate immunity in asian sea bass (lates calcarifer) and defense against streptococcus iniae. | oligodeoxynucleotides (odns) containing unmethylated cytosine-phosphate-guanine cpg dinucleotides within specific sequence contexts (cpg motifs) have been reported as pathogen-associated molecular patterns (pamps). its immunostimulatory effects have been demonstrated in diverse vertebrate models. cpg odn is typically found in bacterial or viral genome and recognized by a non-self recognition receptor toll-like receptor9 (tlr9). here, a new cpg odn 1013 which mimics sequence of ssu rdna of early ... | 2016 | 27629917 |
| streptococcus iniae cpsg alters capsular carbohydrate composition and is a cause of serotype switching in vaccinated fish. | streptococcus iniae causes septicaemia and meningitis in marine and freshwater fish wherever they are farmed in warm-temperate and tropical regions. although serotype specific, vaccination with bacterins (killed bacterial cultures) is largely successful and vaccine failure occurs only occasionally through emergence of new capsular serotypes. previously we showed that mutations in vaccine escapes are restricted to a limited repertoire of genes within the 20-gene capsular polysaccharide (cps) oper ... | 2016 | 27599938 |
| the mucosal expression signatures of g-type lysozyme in turbot (scophthalmus maximus) following bacterial challenge. | the mucosal surfaces constitute the first line of host defense against infection, and also serve as the dynamic interfaces that simultaneously mediate a diverse array of critical physiological processes, while in constantly contact with a wide range of pathogens. the lysozymes are considered as key components for innate immune response to pathogen infection with their strong antibacterial activities. but their activities in mucosal immune responses were always overlooked, especially for g-type l ... | 2016 | 27189917 |
| draft genome sequences for seven streptococcus parauberis isolates from wild fish in the chesapeake bay. | streptococcus parauberis is a pathogen of cattle and fish, closely related streptococcus uberis and streptococcus iniae we report the genomes of seven s. parauberis strains recovered from striped bass (morone saxatilis) in the chesapeake bay. the availability of these genomes will allow comparative genomic analysis of chesapeake bay s. parauberis strains versus s. parauberis cultured from other animal hosts and geographic regions. | 2016 | 27540054 |
| cpsj gene of streptococcus iniae is involved in capsular polysaccharide synthesis and virulence. | the capsular polysaccharides are an important virulence factor of streptococcus iniae, protecting the bacterium from destruction and clearance by the immune system. the cpsj gene encodes a putative udp-glucose epimerase involved in the capsule synthesis system. to determine the role of the cpsj protein in the production of the capsule, a δcpsj mutant was generated and analyzed by comparing its growth performances and virulence with those of the wild type (wt) strain. the δcpsj mutant had longer ... | 2016 | 27535839 |
| characterization and expression analysis of a peptidoglycan recognition protein gene, smpgrp2 in mucosal tissues of turbot (scophthalmus maximus l.) following bacterial challenge. | peptidoglycan recognition receptor proteins (pgrps), a group of pattern recognition receptors (prrs), can recognize peptidoglycan (pgn) of the bacteria cell wall and play an important role in host immune defense against pathogen infection. they are highly structurally conserved through evolution, but with different function in innate immunity between invertebrates and vertebrates. in teleost fish, several pgrps have been characterized recently. they have both amidase activity and bactericidal ac ... | 2016 | 27461422 |
| comprehensive characterization of three glutathione s-transferase family proteins from black rockfish (sebastes schlegelii). | glutathione s-transferases (gsts, ec 2.5.1.18) are categorized as phase ii enzymes, which form an important multifunctional family associated with a wide variety of catalytic activities. gstω, gstρ, and gstθ are cytosolic gsts which have been extensively studied in a variety of organisms; however, few studies have focused on teleosts. those paralogs from black rockfish (sebastes schlegelii; rfgstω, rfgstρ, and rfgstθ, respectively) were molecularly, biochemically, and functionally characterized ... | 2016 | 27449269 |
| molecular cloning, expression and the adjuvant effects of interleukin-8 of channel catfish (ictalurus punctatus) against streptococcus iniae. | interleukin-8 (il-8) as an important cytokine involving in inflammatory and immune response, has been studied as effective adjuvants for vaccines in mammals. however, there are fewer reports about the characterization and adjuvant effects of il-8 in fish. in this study, cloning and sequence analysis of il-8 coding region of channel catfish (ictalurus punctatus) were conducted, mature il-8(rtil-8) was expressed and evaluated for its adjuvant effects on the immunoprotection of subunit vaccine enco ... | 2016 | 27373470 |
| identification and expression analysis of tlr2 in mucosal tissues of turbot (scophthalmus maximus l.) following bacterial challenge. | the pathogen recognition receptors (prrs), which can recognize the conserved pathogen-associated molecular patterns (pamps) of the bacteria, play key roles in the mucosal surfaces for pathogen recognition and activation of immune signaling pathways. however, our understanding of the prrs and their activities in mucosal surfaces in the critical early time points during pathogen infection is still limited. towards to this end, here, we sought to identify the toll-like receptor 2 (tlr2) in turbot a ... | 2016 | 27368539 |
| effect of n-acetyl cysteine and glycine supplementation on growth performance, glutathione synthesis, anti-oxidative and immune ability of nile tilapia, oreochromis niloticus. | an 8-week feeding trial was conducted to evaluate the effect of n-acetyl cysteine (nac) and glycine supplementation on growth performance, glutathione (gsh) synthesis, anti-oxidative and immune ability of nile tilapia, oreochromis niloticus. four practical diets were formulated, control, control +0.2% nac, control +0.5% glycine, control +0.2% nac +0.5% glycine. each diet was randomly assigned to quadruplicate groups of 30 fish (approximately 9.5 g). the weight gain and specific growth rate were ... | 2016 | 27235905 |
| erratum to: identification of some main streptococcus iniae associated proteins: relationship. | 2017 | 28332027 | |
| characterization and expression analysis of chitinase genes (chit1, chit2 and chit3) in turbot (scophthalmus maximus l.) following bacterial challenge. | chitinases are hydrolytic enzymes which have been employed to breakdown chitin coats of pathogenic microorganisms, thereby weaken the defense system of several pathogens and insects. in this regard, we identified the chitinase genes of turbot and characterized their expression patterns in mucosal tissues following vibrio anguillarum and streptococcus iniae challenge. in present study, transcripts of three chitinase genes (chit1, chit2 and chit3) were captured, as well as their protein structures ... | 2017 | 28286313 |
| the efficacy of amoxicillin sodium against streptococcosis in cultured olive flounder paralichthys olivaceus and its pharmacokinetics. | the efficacy of amoxicillin sodium for controlling field and experimental streptococcus iniae and s. parauberis infections in olive flounder (paralichthys olivaceus) was evaluated after a single intramuscular administration. furthermore, the minimal inhibitory concentrations (mic) against 21 streptococcus strains were determined. in addition, the pharmacokinetics and residue depletion in olive flounder were investigated. single intramuscular doses of amoxicillin sodium at 20, 40, 80, and 160 mg/ ... | 2017 | 27226029 |
| metabolic mechanism for l-leucine-induced metabolome to eliminate streptococcus iniae. | crucial metabolites that modulate hosts' metabolome to eliminate bacterial pathogens have been documented, but the metabolic mechanisms are largely unknown. the present study explores the metabolic mechanism for l-leucine-induced metabolome to eliminate streptococcus iniae in tilapia. gc-ms-based metabolomics was used to investigate the tilapia liver metabolic profile in the presence of exogenous l-leucine. thirty-seven metabolites of differential abundance were determined, and 11 metabolic path ... | 2017 | 28266220 |
| inhibition of mir-92d-3p enhances inflammation responses in genetically improved farmed tilapia (gift, oreochromis niloticus) with streptococcus iniae infection by modulating complement c3. | micrornas (mirnas) are small, non-coding rnas that regulate target gene expression by binding to the 3'-untranslated regions (3'-utrs) of their target mrnas. the mir-92 family is an important mirna family, which was discovered to be related to regulation of tumor proliferation, apoptosis, invasion, and metastasis. inhibition of mir-92d-3p was found previously in head kidney of genetically improved farmed tilapia (gift, oreochromis niloticus) exposed to streptococcus iniae infection. in this stud ... | 2017 | 28235637 |
| effects of exposure to streptococcus iniae on microrna expression in the head kidney of genetically improved farmed tilapia (oreochromis niloticus). | genetically improved farmed tilapia (gift, oreochromis niloticus) are susceptible to infection by streptococcus iniae when maintained in modern intensive culture systems. gift are commercially important fishes that are cultured widely in southern china. the role of micrornas (mirnas) in the regulatory response of gift to s. iniae infection has been underestimated and has not yet been well studied. head kidney has an important immune function in teleost fishes. the main aim of this study was to d ... | 2017 | 28219342 |
| complete genome sequence of streptococcus iniae 89353, a virulent strain isolated from diseased tilapia in taiwan. | streptococcus iniae 89353 is a virulent strain isolated from diseased tilapia in taiwan. the full-genome sequence of s. iniae 89353 is 2,098,647 bp. the revealed genome information will be beneficial for identification and understanding of potential virulence genes of streptococcus iniae and possible immunogens for vaccine development against streptococcosis. | 2017 | 28126946 |
| rapid maldi biotyper-based identification and cluster analysis of streptococcus iniae. | streptococcus iniae causes severe mortalities among cultured marine species, especially in the olive flounder (paralichthys olivaceus), which is economically important in korea and japan. recently, there has been growing concern regarding the emergence of s. iniae as a zoonotic pathogen. here, 89 s. iniae isolates obtained from diseased olive flounders collected from 2003 to 2008 in jeju island, south korea, were characterized using matrix-assisted laser desorption ionization-time of flight mass ... | 2017 | 28124778 |
| fatal bacterial septicemia in a bottlenose dolphin tursiops truncatus caused by streptococcus iniae. | a captive 8 yr old male bottlenose dolphin tursiops truncatus succumbed to septicemia with multisystemic inflammation including suppurative enteritis, encephalitis, and pneumonia with chronic pancreatitis. a pure culture of beta-hemolytic, catalase- and oxidase-negative, gram-positive cocci was isolated from the hilar lymph nodes and pancreas. the isolate was identified by 16s rdna sequencing as streptococcus iniae. histological examination of the digestive system revealed a mixed infection of b ... | 2017 | 28117298 |
| identification of some main streptococcus iniae associated proteins: relationship. | the surface-associated proteins play a key role in bacterial physiology and pathogenesis, and are the major targets in the development of new vaccines. these proteins contribute to the adaptation of bacteria to different hosts and environments. to study differences at the genomic level, we first sequenced the whole genome of streptococcus iniae from fish (iusa-1 strain) and compared it to streptococcus iniae from human (9117 strain), revealing a high similitude between both strains. to gain furt ... | 2017 | 28091882 |
| complete genome sequence of streptococcus iniae uel-si1, isolated in diseased nile tilapia (oreochromis niloticus) from northern paraná, southern brazil. | the streptococcus iniae uel-si1 strain was isolated from diseased nile tilapia within the paranapanema river basin, northern paraná, brazil. this is an emerging infectious disease agent of fish from brazil, and sequencing of the complete genome is fundamental to understanding aspects relative to pathogenesis, infection, epidemiology, and immunity. | 2017 | 28082497 |
| comparative analysis of two thioredoxin-like genes in black rockfish sebastes schlegelii and their possible involvement in redox homeostasis and innate immune responses. | elevated levels of ros can cause serious intracellular damages by reacting readily with nucleic acids, proteins and lipids, thus triggering tissue damage and cell death. thioredoxin system is one of the principal factors that maintain the intracellular redox balance via its antioxidant property. in this study, we characterized two new thioredoxin isoforms (sstxn-like 1 and ssmttxn-like) from black rockfish, sebastes schlegelii. the molecular and structural characteristics, as well as the evoluti ... | 2017 | 27984102 |
| the transcriptional regulator cpsy is important for innate immune evasion in streptococcus pyogenes. | as an exclusively human pathogen, streptococcus pyogenes (the group a streptococcus [gas]) has specifically adapted to evade host innate immunity and survive in multiple tissue niches, including blood. gas can overcome the metabolic constraints of the blood environment and expresses various immunomodulatory factors necessary for survival and immune cell resistance. here we present our investigation of one such factor, the predicted lysr family transcriptional regulator cpsy. the encoding gene, c ... | 2017 | 27993974 |
| hemato-immunological responses and disease resistance in siberian sturgeon acipenser baerii fed on a supplemented diet of lactobacillus plantarum. | a feeding trial was conducted to investigate the effects of different levels of dietary lactobacillus plantarum on hemato-immunological parameters and resistance against streptococcus iniae infection in juvenile siberian sturgeon acipenser baerii. fish (14.6 ± 2.3 g) were fed three experimental diets prepared by supplementing a basal diet with l. plantarum at different concentrations [1 × 10(7), 1 × 10(8) and 1 × 10(9) colony-forming units (cfu) g(-1)] and a control (non-supplemented basal) diet ... | 2017 | 27619367 |
| molecular characterization of a bactericidal permeability-increasing protein/lipopolysaccharide-binding protein from black rockfish (sebastes schlegelii): deciphering its putative antibacterial role. | bactericidal permeability-increasing protein (bpi)/lipopolysaccharide (lps) binding proteins (lbps) are well-known proteins that play an indispensable role in host antimicrobial defense. herein, we report a homolog of bpi/lbp from black rockfish (sebastes schlegelii) (designated as rfbpi/lbp) and characterize its structural and functional features at the molecular level. we identified the putative complete open reading frame (1422 bp) of rflbp that encodes a 474 amino acid protein with a predict ... | 2017 | 27663679 |
| development of a colloidal gold immunochromatographic strip for rapid detection of streptococcus agalactiae in tilapia. | a colloidal gold immunochromatographic strip was developed for rapid detection of streptococcus agalactiae (s. agalactiae) infection in tilapia. the monoclonal antibodies (mab) 4c12 and 3a9 were used to target s. agalactiae as colloidal gold-mab conjugate and captured antibody, respectively. the colloidal gold immunochromatographic strip was assembled via routine procedures. optimal ph and minimum antibody levels in the reaction system for gold colloidal-mab 4c12 conjugation were ph 7.4 and 18μg ... | 2017 | 27992801 |
| co-infection of acipenserid herpesvirus 2 (acihv-2) and streptococcus iniae in cultured white sturgeon acipenser transmontanus. | a mortality event in cultured white sturgeon acipenser transmontanus (richardson, 1836) sub-adults was investigated. after transfer between farms, high mortality was observed in fish, associated with back arching, abnormal swimming, and ulcerative skin lesions. necropsy of moribund individuals revealed hemorrhagic ascites and petechial hemorrhages in the coelomic peritoneum and serosa of internal organs. acipenserid herpesvirus 2 (acihv-2) was isolated from external tissue samples, then identifi ... | 2017 | 28357982 |
| molecular cloning and expression analysis of nod-like receptor 5 in japanese flounder (paralichthys olivaceus) after injection with two different formalin-killed pathogenic bacteria and poly (i:c). | nod-like receptors (nlrs) are members of pattern recognition receptors (prrs) recognized intracellular pathogens. here, we identified a type of nlr with a card domain (nlrc5) in japanese flounder, paralichthys olivaceus (jfnlrc5). the coding sequence jfnlrc5 is 5529 bp long and encodes a protein of 1842 deduced amino acid residues. jfnlrc5 transcripts were highly detected in gills, intestine and spleen of healthy fish. in japanese flounder stimulated with poly (i:c), jfnlrc5 was significantly up ... | 2017 | 27592048 |
| emerging causes of superficial and invasive infections following marine injuries and exposures. | soft tissue bacterial infections following aquatic animal bites, stings, and minor injuries occur commonly and usually on the extremities in fishermen and beachgoers worldwide after freshwater and saltwater exposures. louisiana has more tidal, saltwater, and brackish water shorelines (more than 7,000 miles) than any other state, including alaska and hawaii. as a result, louisiana residents are often exposed to marine pathogens when fishing or working offshore or when enjoying louisiana's miles o ... | 2017 | 25075726 |
| two distinct cxc chemokine receptors (cxcr3 and cxcr4) from the big-belly seahorse hippocampus abdominalis: molecular perspectives and immune defensive role upon pathogenic stress. | cxc chemokine receptor 3 (cxcr3) and 4 (cxcr4) are members of the seven transmembrane g protein coupled receptor family, involved in pivotal physiological functions. in this study, seahorse cxcr3 and cxcr4 (designated as hacxcr3 and hacxcr4) cdna sequences were identified from the transcriptome library and subsequently molecularly characterized. hacxcr3 and hacxcr4 encoded 363 and 373 amino acid long polypeptides, respectively. the hacxcr3 and hacxcr4 deduced proteins have typical structural fea ... | 2017 | 28341456 |
| bacteriophage remediation of bacterial pathogens in aquaculture: a review of the technology. | bacteriophages have been proposed as an alternative to antibiotic usage and several studies on their application in aquaculture have been reported. this review highlights progress to date on phage therapies for the following fish and shellfish diseases and associated pathogens: hemorrhagic septicemia (aeromonas hydrophila) in loaches, furunculosis (aeromonas salmonicida) in trout and salmon, edwardsiellosis (edwardsiella tarda) in eel, columnaris disease (flavobacterium columnare) in catfish, ra ... | 2017 | 26713223 |
| new metabolites from the sponge-derived fungus aspergillus sydowii j05b-7f-4. | two new metabolites, diorcinolic acid (1) and β-d-glucopyranosyl aspergillusene a (8), together with six diphenylethers (2-7), a diketopiperazine (9), a chromone (10) and a xanthone (11) were isolated from the fungus aspergillus sydowii derived from the marine sponge stelletta sp. the planar structures and their relative configurations were elucidated by analysing 1d, 2d nmr and hresims data. compound 8 is the first glycoside of phenolic bisabolane sesquiterpenes. compounds 1 and 8 exhibited mil ... | 2017 | 28278674 |
| identification and molecular profiling of dc-sign-like from big belly seahorse (hippocampus abdominalis) inferring its potential relevancy in host immunity. | dendritic-cell-specific icam-3-grabbing non-integrin (dc-sign) is a c-type lectin that functions as a pattern recognition receptor by recognizing pathogen-associated molecular patterns (pamps). it is also involved in various events of the dendritic cell (dc) life cycle, such as dc migration, antigen capture and presentation, and t cell priming. in this study, a dc-sign-like gene from the big belly seahorse hippocampus abdominalis (designated as shdcs-like) was identified and molecularly characte ... | 2017 | 28867209 |
| molecular cloning, biochemical characterization, and expression analysis of two glutathione s-transferase paralogs from the big-belly seahorse (hippocampus abdominalis). | glutathione s-transferases (gsts, ec 2.5.1.18) are important phase ii detoxifying enzymes that catalyze hydrophobic, electrophilic xenobiotic substance with the conjugation of reduced glutathione (gsh). in this study, gstμ and gstρ paralogs of gst in the big belly seahorse (hippocampus abdominalis; hagstρ, hagstμ) were biochemically, molecularly, functionally characterized to determine their detoxification range and protective capacities upon different pathogenic stresses. hagstρ and hagstμ are ... | 2017 | 28882453 |
| possible probiotic lactic acid bacteria isolated from oysters (crassostrea gigas). | we attempted to isolate lactic acid bacteria (lab) from the marine oyster (crassostrea gigas) and selected several environmental stress-resistant isolates for the development of a future probiotic adjuvant for marine aquaculture. twenty-six presumptive lab isolates were extracted from oysters and screened (by an agar diffusion assay) for antimicrobial activity toward various pathogens: vibrio parahaemolyticus, streptococcus iniae, and edwardsiella tarda. eight isolates had an antibacterial activ ... | 2017 | 28875385 |
| use of maldi-tof mass spectrometry for the fast identification of gram-positive fish pathogens. | gram-positive cocci, such as streptococcus agalactiae, lactococcus garvieae, streptococcus iniae, and streptococcus dysgalactiae subsp. dysgalactiae, are found throughout the world, particularly in outbreaks in farmed fish, and are thus associated with high economic losses, especially in the cultivation of nile tilapia. the aim of this study was to evaluate the efficacy of matrix-assisted laser desorption ionization (maldi)-time of flight (tof) mass spectrometry (ms) as an alternative for the di ... | 2017 | 28848512 |
| protective responses of two paralogs of granulocyte colony stimulating factor (gcsf) in rock bream, oplegnathus fasciatus during bacterial and viral infection. | granulocyte colony stimulating factor (gcsf) has a key role in the production of neutrophilic granulocytes during normal hematopoietic development and release of neutrophils into the blood circulation. in this study we have identified and characterized two paralogs of gcsf (rbgcsf) in rock bream. although rbgcsf-1 and rbgcsf-2 share low sequence conservation, its domains and protein structure still share significant similarity. basal levels of rbgcsf-1 gene expression was high in peripheral bloo ... | 2017 | 28408239 |
| in vitro and in vivo assessments of rhodomyrtus tomentosa leaf extract as an alternative anti-streptococcal agent in nile tilapia (oreochromis niloticus l.). | rhodomyrtustomentosa is a thai medicinal plant that has been attracting attention for its remarkable antibacterial properties against gram-positive pathogenic bacteria. the purpose of this study was to evaluate the antibacterial properties of r. tomentosa leaf extract against streptococcus agalactiae and streptococcus iniae isolated from infected tilapia. | 2017 | 28425874 |
| isolation and pathogenicity of streptococcus iniae in cultured red hybrid tilapia (oreochromis sp.) in malaysia. | this study describes the isolation and pathogenicity of streptococcus iniae in cultured red hybrid tilapia (oreochromis niloticus x o. mossambicus) in malaysia. the isolated gram-positive s. iniae appeared punctiform, transparently white, catalase and oxidase negative and produced complete β-hemolysis on blood agar, while a pcr assay resulted in the amplification of the 16 s rrna gene and lactate oxidase encoded genes. the isolate was sensitive to tetracycline, vancomycin and bacitracin, but wer ... | 2017 | 28787246 |
| identification and expression analysis of fetuin b (fetub) in turbot (scophthalmus maximus l.) mucosal barriers following bacterial challenge. | fetuin b (fetub), a recently described cysteine proteinase inhibitor, has numerous conserved n-glycosylation sites, species-specific o-glycosylation sites, and two cystatin (cy) domains. fetub is likely to play regulatory roles in acute inflammation, female infertility, fish organogenesis and tumor suppression. in the present study, transcript of turbot fetub gene was captured, its protein structure and expression patterns in different tissues with emphasis on mucosal barriers following differen ... | 2017 | 28732765 |
| identification, characterization and expression analysis of tlr5 in the mucosal tissues of turbot (scophthalmus maximus l.) following bacterial challenge. | tlrs (toll-like receptors) are very important pathogen pattern recognition receptors, which control the host immune responses against pathogens through recognition of molecular patterns specific to microorganisms. in this regard, investigation of the turbot tlrs could help to understand the immune responses for pathogen recognition. here, transcripts of two tlr5 (tlr5a and tlr5b) were captured, and their protein structures were also predicted. meanwhile, we characterized their expression pattern ... | 2017 | 28705722 |
| dietary arachidonic acid affects immune function and fatty acid composition in cultured rabbitfish siganus rivulatus. | the marbled spinefoot rabbitfish (siganus rivulatus) is an economically valuable fish species that has potential for commercial production in aquaculture. to overcome challenges in its sustainable production, a formulated diet is required for imparting health and robustness. this study evaluates the effect of dietary supplementation with arachidonic acid (ara; 20:4n-6) on growth, survival, immune function and fatty acid composition of red blood cells (rbcs) in rabbitfish. we conducted two feedin ... | 2017 | 28684322 |
| neutrophil derived ltb4 induces macrophage aggregation in response to encapsulated streptococcus iniae infection. | immune cells sense and react to a multitude of factors including both host and microbe-derived signals. understanding how cells translate these cues into particular cellular behaviors is a complex yet critical area of study. we have previously shown that both neutrophils and macrophages are important for controlling the fish pathogen streptococcus iniae. here, we report both host and bacterial determinants leading to the formation of organized macrophage aggregates as part of the host inflammato ... | 2017 | 28658259 |
| molecular, transcriptional and functional insights into duplicated goose-type lysozymes from sebastes schlegelii and their potential immunological role. | black rockfish (sebastes schlegelii), an important aquaculture species in korea, has been affected by bacterial diseases leading to a drastic decline in production. goose-type lysozyme (lysg) is a key enzyme of the innate immune system to eradicate bacterial infections. in this study, two isoforms of lysg from black rockfish, designated as rflysg1 and rflysg2, have been identified and characterized at the molecular, transcriptional, and functional levels. the deduced amino acid sequences had the ... | 2017 | 28528707 |
| the involvement of cathepsin f gene (ctsf) in turbot (scophthalmus maximus l.) mucosal immunity. | cathepsin f (ctsf) is a recently described papain-like cysteine protease and unique among cathepsins due to an elongated n-terminal pro-region, which contains a cystatin domain. ctsf likely plays a regulatory role in processing the invariant chain which is associated with the major histocompatibility complex (mhc) class ii. in this regard, we identified the ctsf gene of turbot as well as its protein structure, phylogenetic relationships, and expression patterns in mucosal tissues following vibri ... | 2017 | 28501446 |
| molecular insights of two stat1 variants from rock bream (oplegnathus fasciatus) and their transcriptional regulation in response to pathogenic stress, interleukin-10, and tissue injury. | signal transducers and activators of transcription 1 (stat1) is critically involved in mediating cytokine-driven signaling, and triggers the transcription of target genes to activate cellular functions. although the structural and functional aspects of stat members have been well described in mammals, only limited information is available for the stat genes in teleost fishes. in the present study, two variants of stat1 genes (rbstat1 and rbstat1l) were identified from rock bream and characterize ... | 2017 | 28818616 |
| manganese-superoxide dismutase (mnsod), a role player in seahorse (hippocampus abdominalis) antioxidant defense system and adaptive immune system. | manganese superoxide dismutase (mnsod) is a metaloenzyme that catalyzes dismutation of the hazardous superoxide radicals into less hazardous h2o2 and h2o. here, we identified a homolog of mnsod from big belly seahorse (hippocampus abdominalis; hamnsod) and characterized its structural and functional features. hamnsod transcript possessed an open reading frame (orf) of 672 bp which codes for a peptide of 223 amino acids. pairwise alignment showed that hamnsod shared highest identity with rock bre ... | 2017 | 28743628 |
| molecular, structural, and functional comparison of n lobe and c lobe of the transferrin from rock bream, oplegnathus fasciatus, with respect to its immune response. | the iron-withholding strategy of innate immunity is an effective antimicrobial defense mechanism that combats microbial infection by depriving microorganisms of fe(3+), which is important for their growth and propagation. transferrins (tfs) are a group of iron-binding proteins that exert their antimicrobial function through fe(3+) sequestration. the current study describes both structural and functional characteristics of a transferrin ortholog from rock bream oplegnathus fasciatus (rbtf). the r ... | 2017 | 28732766 |
| the first report of cathepsin a gene in a teleost (rock bream, oplegnathus fasciatus): an investigation of immune functions upon infection with several pathogens. | we isolated and characterised a cdna encoding the lysosomal protective protein (serine protease) cathepsin a (ctsa) from rock bream (oplegnathus fasciatus). the full-length rock bream ctsa (rbctsa) cdna (1814 bp) contains an open reading frame of 1419 bp that encodes 472 amino acids. alignment of multiple ctsa protein sequences revealed that the active site serine and histidine residues were well-conserved among the other ctsa sequences. rbctsa is highly expressed in the peripheral blood leukocy ... | 2017 | 28624491 |
| a cxcl ortholog from hippocampus abdominalis: molecular features and functional delineation as a pro-inflammatory chemokine. | chemokines are a family of chemotactic cytokines that regulate leukocyte migration. they are classified into four groups namely, cxc, cc, c and cx3c, based on the formation of a disulfide bridge. among these, cxc chemokines have been identified as the largest group of chemokines in humans. in this study, we identified and functionally characterized a homolog of cxc chemokine from the big-belly seahorse, hippocampus abdominalis, and designated it as shcxcl. the cdna of shcxcl composed of a 342-bp ... | 2017 | 28546023 |
| molecular cloning, transcriptional profiling, and subcellular localization of signal transducer and activator of transcription 2 (stat2) ortholog from rock bream, oplegnathus fasciatus. | signal transducer and activator of transcription 2 (stat2) is a key element that transduces signals from the cell membrane to the nucleus via the type i interferon-signaling pathway. although the structural and functional aspects of stat proteins are well studied in mammals, information on teleostean stats is very limited. in this study, a stat paralog, which is highly homologous to the stat2 members, was identified from a commercially important fish species called rock bream and designated as r ... | 2017 | 28499943 |
| characterization of toll-like receptor 22 in turbot (scophthalmus maximus). | toll-like receptors (tlrs) are essential for activation of the innate immune system in response to invading pathogens. in this paper, expression profiles of the turbot (scophthalmus maximus) tlr22 gene (tbtlr22) were analyzed with rt-pcr and in situ hybridization. then its expression patterns simulated with ligands or pathogens were investigated. streptococcus iniae, edwardsiella tarda, hirame rhabdovirus virus (hirrv), polyinosinic: polycytidylic acid (poly i:c), peptidoglycan (pgn), or lipopol ... | 2017 | 28495564 |
| a homolog of teleostean signal transducer and activator of transcription 3 (stat3) from rock bream, oplegnathus fasciatus: structural insights, transcriptional modulation, and subcellular localization. | signal transducer and activator of transcription 3 (stat3) is one of the crucial transcription factors in the janus kinase (jak)/stat signaling pathway, and it was previously considered as acute phase response factor. a number of interleukins (ils) such as il-5, il-6, il-9, il-10, il-12, and il-22 are known to be involved in activation of stat3. in addition, various growth factors and pathogenic or oxidative stresses mediate the activation of a wide range of functions via stat3. in this study, a ... | 2017 | 28413047 |
| identification and screening of effective protective antigens for channel catfish against streptococcus iniae. | vaccination is a potential approach for prevention and control of disease in fish. the use of genetically engineered vaccines is an effective method and a green intervention to control bacterial infection in aquaculture. however, efforts to develop these vaccines are limited by the lack of conserved protective antigens. in this study, three candidate immunogens (srr, neua, and hsp) of the pathogenic streptococcus iniae strain dgx07 isolated from diseased channel catfish were identified and analy ... | 2017 | 28415641 |
| a thioredoxin domain-containing protein 12 from black rockfish sebastes schlegelii: responses to immune challenges and protection from apoptosis against oxidative stress. | thioredoxin (txn) superfamily proteins are identified by the presence of a thioredoxin active site with a conserved cxxc active motif. txn members are involved in a wide range of biochemical and biological functions including redox regulation, refolding of disulfide containing proteins, and regulation of transcription factors. in the present study, a thioredoxin domain-containing protein 12 was identified and characterized from black rockfish, sebastes schlegelii (rftxndc12). the full length of ... | 2017 | 26945103 |
| antibacterial activities of viriditoxin congeners and synthetic analogues against fish pathogens. | viriditoxin is a fungal secondary metabolite of the fungus paecilomyces variotii derived from the inner tissues of the giant jellyfish nemopilema nomurai. viriditoxin exhibits antibacterial activity against streptococcus iniae and streptococcus parauberis, which are major pathogens of aqua cultured fish. viriditoxin induced abnormal cell morphologies in the fish pathogens s. iniae and s. parauberis, presumably by inhibiting ftsz polymerization as was previously observed in escherichia coli. synt ... | 2017 | 29037949 |
| immunological responses and disease resistance of rainbow trout (oncorhynchus mykiss) juveniles following dietary administration of stinging nettle (urtica dioica). | the present study investigated the effects of dietary supplementation of stinging nettle (urtica dioica) on growth performance, skin mucus, immune response and disease resistance of rainbow trout (oncorhynchus mykiss) fed with diets supplemented with u. dioica at 0, 1, 2 and 3%. after 8 weeks of feeding, the addition of u. dioica at 3% level resulted in improved weight gain, specific growth rate and feed conversion ratio significantly when compared to the other groups (p < 0.05). hematological r ... | 2017 | 29017944 |
| modulatory effect of different doses of β-1,3/1,6-glucan on the expression of antioxidant, inflammatory, stress and immune-related genes of oreochromis niloticus challenged with streptococcus iniae. | β-glucans are widely-known immunostimulants that are profusely used in aquaculture industry. the present study was conducted to evaluate the effect of different in-feed doses of β-1,3/1,6-glucans on the expression of antioxidant and stress-related genes (gst, hsp-70, vtg), inflammation related genes (il-8, tnfα, cxc-chemokine and cas) and adaptive immune-related genes (mhc-iiβ, tlr-7, igm-h, and mx) of oreochromis niloticus challenged and non-challenged with streptococcus iniae. six experimental ... | 2017 | 28882806 |
| insights into the multifunctional role of natural killer enhancing factor-a (nkef-a/prx1) in big-belly seahorse (hippocampus abdominalis): dna protection, insulin reduction, h2o2 scavenging, and immune modulation activity. | natural killer enhancing factor a (nkef-a), also known as peroxiredoxin 1 (prx1), is a well-known antioxidant involved in innate immunity. although nkef-a/prx1 has been studied in different fish species, the present study broadens the knowledge of nkef-a gene in terms of molecular structure, function, and immune responses in fish species. hippocampus abdominalis nkef-a (hankef-a) cdna encoded a putative protein of 198 amino acids containing a thioredoxin_2 domain, vcp motifs, and three conserved ... | 2018 | 29155258 |
| a comparative study of three akirin genes from big belly seahorse hippocampus abdominalis: molecular, transcriptional and functional characterization. | akirins, members of the nf-κb signaling pathway, are highly conserved nuclear proteins, which regulate gene expression in many physiological processes, including immunity, myogenesis, carcinogenesis, and embryogenesis. the akirin family in teleost fish consists of two to three genes. in the present study, three akirin genes from hippocampus abdominalis were identified from a transcriptome database and designated as haakirin1, haakirin2(1), and haakirin2(2). the nuclear localization of haakirin1 ... | 2018 | 29355762 |
| first identification and characterization of streptococcus iniae obtained from tilapia (oreochromis aureus) farmed in mexico. | this is the first study to isolate, identify and characterize streptococcus iniae as the causative disease agent in two tilapia (oreochromis aureus) populations. the populations were geographically isolated, of distinct origins, and did not share water sources. affected fish showed various external (e.g., exophthalmia and cachexia, among others) and internal (e.g., granulomatous septicaemia and interstitial nephritis, among others) signs. all internal organ samples produced pure cultures, two of ... | 2018 | 29315698 |
| characterization of a kazal-type serine protease inhibitor from black rockfish sebastes schlegelii and its possible role in hepatic immune response. | kazal-type serine protease inhibitors (kspis) play important roles in the regulation of endogenous proteases, cell development, blood coagulation, and immune response. in this study, we identified and characterized a kspi homologue (sskspi) in black rockfish, sebastes schlegelii. the full-length cdna sequence of sskspi was 532 base pairs (bp), including an open reading frame (orf) of 330 bp, which encodes a polypeptide of 110 amino acids with a signal peptide of 21 amino acids. the greatest valu ... | 2018 | 29305992 |
| preparation, characterization and evaluation of the immune effect of alginate/chitosan composite microspheres encapsulating recombinant protein of streptococcus iniae designed for fish oral vaccination. | streptococcus iniae has caused serious harm to the fish farming industry in recent years. vaccination is a potential approach for preventing and controlling disease, being oral vaccination the most suitable vaccination route in fish. alginate and chitosan microspheres have been widely used as controlled release systems for oral vaccination in fish. in this study, we prepared and characterized alginate/chitosan composite microspheres encapsulating the recombinant protein serine-rich repeat (rsrr) ... | 2018 | 29275131 |
| gibson assembly facilitates bacterial allelic exchange mutagenesis. | allelic exchange mutagenesis that relies on reca-mediated homologous recombination up- and downstream from the targeted gene is a generalizable method of site-specific bacterial gene knock-out and knock-in. however, generation of a mutagenic dna construct (alternative allele flanked by regions surrounding the gene target) and subsequent mutant selection are laborious procedures. here we demonstrate allelic exchange knock-out facilitated by gibson assembly in streptococcus iniae. gibson assembly ... | 2018 | 29196271 |
| snps in the toll1 receptor of litopenaeus vannamei are associated with immune response. | tolls and toll-like receptors (tlrs) are important regulators in the innate immune system and their genetic variations usually affect the host's susceptibility/resistance to pathogen infections. in this study, we report on the single nucleotide polymorphisms (snps) of toll1 in litopenaeus vannamei (lvtoll1) and how this is associated with immune response. pcr-dgge analysis revealed genetic polymorphisms in lvtoll1 at both the genomic dna (gdna) and cdna levels. using high-throughput sequencing, ... | 2018 | 29146444 |
| ccd and rsm optimization approach for antioxidative activity and immune regulation in head kidney of yellow catfish (pelteobagrus fulvidraco) based on different lipid levels and temperatures. | yellow catfish (pelteobagrus fulvidraco) is an important economic cultured fish in china. here we report antioxidative activity and immune regulation in head kidney using a central composite design based on water temperature (20-34 °c) and dietary lipid (2-17%). response values were optimized using response surface methodology to maximize the immune response and relieve oxidative stress. the experiment was conducted under laboratory conditions and lasted for seven weeks. the results showed that ... | 2018 | 29107064 |