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molecular epidemiology of streptococcus bovis causing endocarditis and bacteraemia in italian patients.streptococcus bovis is being recognised increasingly as a cause of infective endocarditis, and has also been associated with underlying gastrointestinal malignancy. this study evaluated the molecular epidemiology of s. bovis isolates responsible for endocarditis or bacteraemia in italian patients between january 1990 and august 2003. s. bovis isolates were classified on the basis of their biochemical profiles, antimicrobial susceptibilities and genotypes. of 25 isolates studied, 20 were s. bovis ...200516153255
[bacterially conditioned thromboembolism in dairy cows--a retrospective study of 31 necropsy cases with special consideration of the causative complex].a study of 340 necropsied dairy cattle from northern bavaria resulted in 31 animals (9%) showing evidence of pyemic thromboembolism. the most frequent pathomorphologic lesions consisted in endocarditis valvularis thromboticans of the tricuspid and/or mitral valve (21), embolic pneumonia (26), nephritis (13) and renal infarction (12). the most common isolate found in bacteriologic culture was arcanobacterium pyogenes in 26 of 31 cases, followed by staphylococcus aureus (2), mannheimia haemolytica ...200515803759
[endocarditis due to streptococcus bovis and hepatic disease]. 200516143090
characterization of culturable anaerobic bacteria from the forestomach of an eastern grey kangaroo, macropus giganteus.to determine the culturable biodiversity of anaerobic bacteria isolated from the forestomach contents of an eastern grey kangaroo, macropus giganteus, using phenotypic characterization and 16s rdna sequence analysis.200516162139
cerebral ischemia caused by streptococcus bovis aortic endocarditis: case report.cerebral ischemic processes associated with infective endocarditis caused by streptococcus bovis are rare; only 2 cases having been reported. here we report a case of a 50-year-old man with s. bovis endocarditis who presented signs of frontal, parietal and occipital lobe cerebral ischemia. this is the first case reported in which the presence of hemianopsia preceded the endocarditis diagnosis. initially, the clinical manifestations suggested a systemic vasculitis. later, vegetating lesions were ...200516172722
mitral valve endocarditis after dental prophylaxis in a dog. 200515822575
the erm(t) gene is flanked by is1216v in inducible erythromycin-resistant streptococcus gallolyticus subsp. pasteurianus.we investigated the sequence and the genetic context of the erm(t) gene in six inducible erythromycin-resistant streptococcus gallolyticus subsp. pasteurianus (formerly s. bovis biotype ii.2) isolates. in all isolates, the erm(t) genes were flanked by two is1216v-like elements with the same polarity and were found to be inserted in the chromosome.200516189118
observations on salpingitis, peritonitis and salpingoperitonitis in a layer breeder flock.a flock of 13,951 hens and 1379 cockerels was monitored from 26 to 58 weeks of age for the complex of salpingitis, peritonitis and salpingoperitonitis (sps). two hundred and forty-three hens (78 per cent of the hens that died) were examined postmortem, and sps was recognised by gross examination for inflammatory exudate, in the body cavity or oviduct in 111 (46 per cent) of them. salpingoperitonitis was the most common form, followed by salpingitis and then peritonitis. there were acute and chro ...200516272543
bacteremia due to streptococcus gallolyticus, or the perils of revised nomenclature in bacteriology. 200515825011
genetic basis of antibiotic resistance in clinical isolates of streptococcus gallolyticus (streptococcus bovis).among 128 streptococcus gallolyticus (streptococcus bovis) isolates, 77.7% were resistant to tetracyclines and contained tet(m) and/or tet(l) and/or tet(o). a total of 59.4% had macrolide resistance and contained erm(b) and, rarely, mef(a). among the one-third of isolates highly resistant to kanamycin and streptomycin, most harbored apha3 and aad-6 genes.200515793162
gross abnormalities, bacteriology and histological lesions of uteri of dairy cows failing to conceive or maintain pregnancy.to describe the gross pathology, bacteriology and histopathology of the reproductive tracts of dairy cows that failed to conceive or maintain pregnancy.200516044186
a retained intravascular medical device and streptococcus bovis infection, providing early diagnosis of an occult malignancy. 200515667921
in vitro activity of telithromycin against viridans group streptococci and streptococcus bovis isolated from blood: antimicrobial susceptibility patterns in different groups of species.the in vitro activities of penicillin, erythromycin, clindamycin, and telithromycin were determined against 155 viridans group streptococci (vgs) and 18 streptococcus bovis blood isolates. heterogeneity in the susceptibility patterns and macrolide resistance phenotypes and genotypes in the different groups of vgs was detected. we found seven telithromycin-resistant s. bovis isolates all harboring the erm(b) gene.200515673778
risk of embolism and death in infective endocarditis: prognostic value of echocardiography: a prospective multicenter study.the incidence of embolic events (ee) and death is still high in patients with infective endocarditis (ie), and data about predictors of these 2 major complications are conflicting. moreover, the exact role of echocardiography in risk stratification is not well defined.200515983252
aetiological diagnosis of infective endocarditis by direct amplification of rrna genes from surgically removed valve tissue. an 11-year experience in a finnish teaching hospital.the aetiology of infective endocarditis (ie) can be determined directly from surgically removed valve tissue using broad-range bacterial rdna polymerase chain reaction (pcr) followed by sequencing. we sought to assess the value of this methodology in a routine clinical setting.200616754257
display of active enzymes on the cell surface of escherichia coli using pgsa anchor protein and their application to bioconversion.we have developed a novel escherichia coli cell surface display system by employing pgsa as an anchoring motif. in our display system, c-terminal fusion to pgsa anchor protein from bacillus subtilis was used. the enzymes selected for display were alpha-amylase (amya) from streptococcus bovis 148 and lipase b (calb) from candida antarctica. the molecular mass values of amya and calb are approximately 77 and 34 kda, respectively. the enzymes were displayed on the surface as a fusion protein with a ...200616133338
effects of feeding polyclonal antibody preparations on ruminal bacterial populations and ruminal ph of steers fed high-grain diets.three experiments with factorial arrangements of treatments were designed to test the efficacy of avian-derived polyclonal antibody preparations (pap) against streptococcus bovis (pap-sb) or fusobacterium necrophorum (pap-fn) in reducing ruminal counts of target bacteria in beef steers supplemented or not with feed additives (300 mg of monensin/d and 90 mg of tylosin/d; mt). feeding increasing doses of pap-sb in exp. 1 or a single dose in exp. 2 reduced s. bovis counts in a cubic fashion (p = 0. ...200616864880
inflammatory bowel disease and streptococcus bovis.evidence suggests a trend for a higher fecal carriage rate of streptococcus bovis in adult patients with inflammatory bowel disease (ibd). this study defines the fecal carriage rate of s. bovis among children with ibd compared to controls. subjects with ibd were prospectively enrolled from the patient population of a pediatric gastroenterology practice. stool samples from ibd patients as well as controls were analyzed within 24 hr for the presence of s. bovis. the study enrolled 47 patients with ...200616868828
streptococcus bovis: causal or incidental involvement in cancer of the colon? 200616947772
streptococcus bovis infection in young infants.sporadic cases of invasive streptococcus bovis disease have been reported in young infants. however, little is known about the clinical manifestations or the conditions that predispose to s. bovis infection in this population.200617072133
presence of nad+-specific glyceraldehyde-3-phosphate dehydrogenase and ccpa-dependent transcription of its gene in the ruminal bacterium streptococcus bovis.to know the role of nadp+-specific glyceraldehyde-3-phosphate dehydrogenase (gapn) in streptococcus bovis, the molecular properties and transcriptional control of the gene encoding gapn (gapn) were examined. the gapn in s. bovis was deduced to consist of 476 amino acids with a molecular mass of 51.1 kda. the gapn gene was transcribed in a monocistronic fashion. gapn synthesis appeared to be regulated at the transcriptional level in response to changes in growth conditions. in a mutant that lacks ...200616553827
changes in equine hindgut bacterial populations during oligofructose-induced laminitis.in the horse, carbohydrate overload is thought to play an integral role in the onset of laminitis by drastically altering the profile of bacterial populations in the hindgut. the objectives of this study were to develop and validate microbial ecology methods to monitor changes in bacterial populations throughout the course of experimentally induced laminitis and to identify the predominant oligofructose-utilizing organisms. laminitis was induced in five horses by administration of oligofructose. ...200616623745
simultaneously performed mitral valve replacement and hartmann's operation for infectious endocarditis from streptococcus bovis and rectal cancer.in patients with surgical indications for both cardiac surgery and a malignant neoplasm, cardiac surgery should generally be performed first; however, in some cases simultaneous surgery is recommended. we report a case of infectious endocarditis of the mitral valve caused by streptococcus bovis with rectal cancer. blood cultures showed a rise in coagulase-negative s. bovis, and the source of infection was thought to be the rectal cancer. the patient presented with cerebral infarction prior to su ...200617037400
activity of dx-619 compared to other agents against viridans group streptococci, streptococcus bovis, and cardiobacterium hominis.against 198 viridans group streptococci, 25 streptococcus bovis strains, and 5 cardiobacterium hominis strains, mics of dx-619, a des-f(6)-quinolone, were between 0.004 and 0.25 microg/ml. these mics were lower than those of other quinolones (< or = 0.008 to > 32 microg/ml). beta-lactam mics were between < or = 0.008 and 16 microg/ml. azithromycin resistance was found in most species, while most were telithromycin susceptible. glycopeptides and linezolid were active against viridans group strain ...200617043120
[native pulmonary and aortic value endocarditis by streptococcus bovis]. 200617080555
abomasal bacteria produce an inhibitor of gastrin secretion in vitro.previously, proliferating microflora transferred with abomasal nematodes, were suspected to be the source of the gastrin inhibitor in some parasite excretory/secretory products. aerobic cultures in hbss of abomasal fluid from uninfected sheep became inhibitory during the static growth phase, unless antibiotics were present. basal gastrin secretion was reduced by up to 90%. rumen fluid and incubates and medium in which streptococcus bovis and ovine rumen actinomycete spp. had been grown also cont ...200616352323
extracellular metabolomics: a metabolic footprinting approach to assess fiber degradation in complex media.this work reports the implementation and optimization of a method for high-throughput analysis of metabolites produced by the breakdown of natural polysaccharides by microorganisms. our simple protocol enables simultaneous separation and quantification of more than 40 different sugars and sugar derivatives, in addition to several organic acids in complex media, using 50-mul samples and a standard gas chromatography-mass spectrometry platform that was fully optimized for this purpose. as an imple ...200616356465
display of alpha-amylase on the surface of lactobacillus casei cells by use of the pgsa anchor protein, and production of lactic acid from starch.we developed a new cell surface engineering system based on the pgsa anchor protein from bacillus subtilis. in this system, the n terminus of the target protein was fused to the pgsa protein and the resulting fusion protein was expressed on the cell surface. using this new system, we constructed a novel starch-degrading strain of lactobacillus casei by genetically displaying alpha-amylase from the streptococcus bovis strain 148 with a flag peptide tag (amyaf). localization of the pgsa-amya-flag ...200616391053
factors affecting the activity of bovicin hc5, a bacteriocin from streptococcus bovis hc5: release, stability and binding to target bacteria.to determine the factors affecting the release, stability and binding of bovicin hc5 to sensitive bacteria.200616405697
epidemiologic, clinical, and microbiologic profile of infective endocarditis in argentina: a national survey. the endocarditis infecciosa en la república argentina-2 (eira-2) study.this study aimed to determine the epidemiologic, clinical, microbiologic characteristics, and inhospital outcome of infective endocarditis (ie) in argentina and compare the results with those of the 1992 ie national survey.200616442929
isolation and identification of mixed linked beta -glucan degrading bacteria in the intestine of broiler chickens and partial characterization of respective 1,3-1,4-beta -glucanase activities.media with 1,3-1,4-beta -glucans as selective markers were used for isolation of non-starch-polysaccharide (nsp) degrading bacteria from the intestinal tract of broiler chicken. formerly unknown 1,3-1,4-beta endoglucanase activities in various bacterial species were identified in this study. e. faecium , streptococcus , bacteroides and clostridium strains seem to be responsible for degradation of mixed linked beta -glucans in the small intestine and in the hind gut of chickens. strict anaerobic ...200616721874
septic discitis as initial manifestation of streptococcus bovis endocarditis.a 68-year-old man with septic discitis of low lumbar spine is presented. the patient suffered an acute hemorrhagic stroke due to rupture of mycotic aneurysm. streptococcus bovis biotype i was found in blood cultures. echocardiography showed bioprosthesis aortic endocarditis. cardiac surgery was not performed because of normal bioprosthesis function and absence of peri-annular complications. the patient was discharged after 6 weeks of antibiotic treatment.200616516710
assessment of biases associated with profiling simple, model communities using terminal-restriction fragment length polymorphism-based analyses.community profiles based on terminal-restriction fragment length polymorphism (t-rflp) analyses of amplified ribosomal rna genes are used to monitor changes in microbial community structure and are sometimes employed for semi-quantitative estimates of species richness and abundance in environmental samples. to assess the accuracy of t-rflp community profiles representing the relative abundance of bacteria in a sample, five species of ruminal bacteria were used to construct simple "communities". ...200616563536
the association of streptococcus bovis bacteremia and gastrointestinal diseases: a retrospective analysis.there is a well-established association between streptococcus bovis bacteremia (sbb) and colorectal cancer. however, sbb is also frequently associated with chronic liver disease and has been described with other gastrointestinal disorders. the aim of the study was to evaluate the prevalence of gastrointestinal disease in patients with sbb. retrospective analysis of the microbiology database at jackson memorial medical center, miami, florida, between 1992 and 2002, was performed. patients' clinic ...200616614996
artisanal and experimental pecorino siciliano cheese: microbial dynamics during manufacture assessed by culturing and pcr-dgge analyses.traditional artisanal pecorino siciliano (ps) cheeses, and two experimental ps cheeses were manufactured using either raw or pasteurised ewes' milk with the addition of starter cultures. the bacterial diversity and dynamics of the different cheese types were evaluated both by culturing and characterisation of isolates, and a culture-independent approach based on the 16s ribosomal rna (rrna) gene. following cultivation, artisanal and experimental cheese types showed similar microbial counts, and ...200616616965
streptococcus bovis bacteremia associated with acute cholecystitis. 200616721235
profiling the humoral immune response in colon cancer patients: diagnostic antigens from streptococcus bovis.the human bowel contains a large and dynamic bacterial population that is not only essential for intestinal health, but also critical for the development of diseases such as cancer. in this respect, the gram-positive bacterium streptococcus bovis has been associated with colon cancer for many years. to investigate the clinical importance of this association, an immunocapture mass spectrometry assay was developed that can generate infection-related protein profiles. the composition of these profi ...200616841330
unusual bacterial infections and colorectal carcinoma--streptococcus bovis and clostridium septicum: report of three cases.several types of unusual bacterial infections have been reported in association with colorectal malignancy. in the three cases described, uncommon infections resulted in further workup and subsequent resection for occult colonic malignancy. awareness of this association is critical for early diagnosis and management.200616845563
management of active infective endocarditis complicated by large vegetations. 200616901055
in vitro bacterial growth and in vivo ruminal microbiota populations associated with bloat in steers grazing wheat forage.the role of ruminal bacteria in the frothy bloat complex common to cattle grazing winter wheat has not been previously determined. two experiments, one in vitro and another in vivo, were designed to elucidate the effects of fresh wheat forage on bacterial growth, biofilm complexes, rumen fermentation end products, rumen bacterial diversity, and bloat potential. in exp. 1, 6 strains of ruminal bacteria (streptococcus bovis strain 26, prevotella ruminicola strain 23, eubacterium ruminantium b1c23, ...200616971591
[the importance of streptococcus bovis systemic infections].streptococcus bovis is relatively common cause of bacteremia and endocarditis, especially in older persons, or in patients that have some kind of chronic disease. these infections are frequently connected with malignant, potentially malignant, or benign colorectal neoplasia. hematogenous dissemination of streptococcus bovis could result with various clinical manifestations, namely purulent meningitis, brain abscess, osteomyelitis, spondylodiscitis, and many different kinds of infections in aids ...200617087134
streptococcus suis endocarditis and colon carcinoma: a case report.streptococcus bovis has classically been associated with colorectal carcinomas in literature. curiously enough, no other streptococcus species have hitherto been associated with such a malignancy or any other tumors. this report presents a case of early-stage colorectal carcinoma diagnosed in a patient with infection with the related streptococcus species s suis. a discussion of possible pathogenetic implications follows.200617026794
the effect of calcium and magnesium on the activity of bovicin hc5 and nisin.some gram-positive bacteria produce small peptides (bacteriocins) that have antimicrobial activity, but many bacteria can become bacteriocin resistant. bovicin hc5, a lantibiotic produced by streptococcus bovis hc5, has the ability to inhibit nisin-resistant bacteria. because nisin resistance has in many cases been correlated with an alteration of lipoteichoic acids or the polar head groups of membrane phospholipids, we decided to examine the effect of divalent cations on nisin and bovicin hc5 a ...200617036211
bacterial competition between a bacteriocin-producing and a bacteriocin-negative strain of streptococcus bovis in batch and continuous culture.a bacteriocin-producing streptococcus bovis strain (hc5) outcompeted a sensitive strain (jb1) before it reached stationary phase (ph 6.4), even though it grew 10% slower and cell-free bovicin hc5 could not yet be detected. the success of bacteriocin-negative s. bovis isolates was enhanced by the presence of another sensitive bacterium (clostridium sticklandii sr). pcr based on repetitive dna sequences indicated that s. bovis hc5 was not simply transferring bacteriocin genes to s. bovis jb1. when ...200617117976
gemella endocarditis: consider the colon.gemella species, including gemella morbillorum, are part of the normal flora of the human oropharynx, upper respiratory tract and gastrointestinal tract. differentiation of gemella from viridans streptococci in the laboratory may be difficult. although uncommonly pathogenic, they have been implicated in a variety of human infections, most notably infective endocarditis. dental instrumentation appears to be the usual source of infection. the case is reported of gemella morbillorum native valve en ...200617152793
late streptococcus bovis infection of knee arthroplasty and its association with carcinoma of the colon: a case report.streptococcus bovis is a very rare cause of infection of joint arthroplasty. infections with s. bovis have been reported to be associated with various gastrointestinal conditions. we present a case of total knee arthroplasty infected with s. bovis 40 months after a successful knee replacement. the diagnosis was established on joint fluid as well as blood cultures. early diagnosis, arthroscopic wash out and appropriate antibiotics helped salvage the prosthesis. early colonoscopy revealed carcinom ...200717072656
pyogenic liver abscess presenting after malignant polypectomy.streptococcus bovis bacteremia has been linked to the presence of occult colon cancer since 1977. we present a case of pyogenic liver abscess and bacteremia with a different streptococcus viridans 1 week after colonic adenocarcinoma was removed via polypectomy, discuss the likely etiology and review whether there is evidence to support looking for colon cancer in patients who present similarly but have not already undergone screening.200717406839
identification of alpha-hemolytic streptococci by pyrosequencing the 16s rrna gene and by use of vitek 2.alpha-hemolytic streptococci are very difficult to identify by phenotypic methods. in this study, a pyrosequencing method for the identification of streptococcal species based on two variable regions of the 16s rrna gene is described. almost all studied streptococcal species (n = 51) represented by their type strains could be differentiated except for some closely related species of the streptococcus bovis or s. salivarius group. the pyrosequencing results of alpha-hemolytic streptococci isolate ...200717215341
production of l-lysine from starch by corynebacterium glutamicum displaying alpha-amylase on its cell surface.we engineered a corynebacterium glutamicum strain displaying alpha-amylase from streptococcus bovis 148 (amya) on its cell surface to produce amino acids directly from starch. we used pgsa from bacillus subtilis as an anchor protein, and the n-terminus of alpha-amylase was fused to the pgsa. the genes of the fusion protein were integrated into the homoserine dehydrogenase gene locus on the chromosome by homologous recombination. l-lysine fermentation was carried out using c. glutamicum displayin ...200717216452
dominance of prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time pcr.relative quantification real-time pcr was used to quantify several bacterial species in ruminal samples from two lactating cows, each sampled 3 h after feeding on two successive days. abundance of each target taxon was calculated as a fraction of the total 16s rrna gene copies in the samples, using taxon-specific and eubacterial domain-level primers. bacterial populations showed a clear predominance of members of the genus prevotella, which comprised 42% to 60% of the bacterial rrna gene copies ...200717235560
fluoroquinolone resistance among invasive viridans group streptococci and streptococcus bovis isolated in spain. 200717296288
[molecular-biological methods of diagnosing colon-related streptococcus bovis endocarditis].bacteremia and infectious endocarditis caused by streptococcus bovis may be related to the presence of inflammation or neoplastic lesions in the large intestine. this report describes a 46-year-old woman who presented with infectious endocarditis caused by s. bovis associated with colonic tubular-villous adenoma. demonstration of bacterial dna by polymerase chain reaction and identification by dna sequencing was performed to establish the diagnosis.200717311758
streptococcus bovis endophthalmitis: a unique presentation of colon cancer. 200717443405
characteristics and regional variations of group d streptococcal endocarditis in france.the proportion of infective endocarditis (ie) caused by group d streptococci (gds; formerly streptococcus bovis) increased markedly in france, to account for 25% of all cases of ie by 1999. in an attempt to explain this phenomenon, a comparative analysis of gds and oral streptococci (os) causing ie was performed. this study was based on data collected from a large cross-sectional population-based survey that was conducted in 1999. in total, 559 cases of definite ie were recorded, of which 142 in ...200717501973
identification of glucose-fermenting bacteria present in an in vitro model of the human intestine by rna-stable isotope probing.16s rrna-based stable isotope probing (sip) and nuclear magnetic resonance (nmr) spectroscopy-based metabolic profiling were used to identify bacteria fermenting glucose under conditions simulating the human intestine. the tim-2 in vitro model of the human intestine was inoculated with a gi tract microbiota resembling that of the small intestine, to which subsequently 4, 20 or 40 mm of [u-(13)c]-glucose were added. rna was extracted from lumen samples after 0 (control), 1, 2 and 4 h and subjecte ...200717313661
bactericidal effect of bovicin hc5 and nisin against clostridium tyrobutyricum isolated from spoiled mango pulp.to test the effect of bovicin hc5--a bacteriocin from streptococcus bovis hc5--against the strains of clostridium tyrobutyricum isolated from canned spoiled mango pulp.200717594463
the energy spilling reactions of bacteria and other organisms.for many years it was assumed that living organisms always utilized atp in a highly efficient manner, but simple growth studies with bacteria indicated that the efficiency of biomass production was often at least 3-fold lower than the amount that would be predicted from standard biosynthetic pathways. the utilization of energy for maintenance could only explain a small portion of this discrepancy particularly when the growth rate was high. these ideas and thermodynamic arguments indicated that c ...200717693707
repeated meningitis as a delayed complication of scoliosis surgery.case report.200717538360
molecular profiling of lactobacillus, streptococcus, and bifidobacterium species in feces of active racehorses.diversity and compositions of the lactobacillus, streptococcus, and bifidobacterium group in the feces of six healthy, actively racing horses (thoroughbreds) were analyzed by using pcr-denaturing gradient gel electrophoresis (dgge) and real-time pcr with primer sets specific for each group. pcr-dgge analysis of the feces showed that lactobacillus equi, lactobacillus johnsonii, a phylogenetic relative of lactobacillus salivarius, a phylogenetic relative of lactobacillus gastricus, and weissella c ...200717726300
[streptococcus bovis: an emerging pathogen]. 200717910852
characterization of a bacteriocin, thermophilin 1277, produced by streptococcus thermophilus sbt1277.to assess the inhibitory activity and the influence of culture condition on the growth and bacteriocin, thermophilin 1277, production by streptococcus thermophilus sbt1277.200717381740
biological and genetic classification of canine intestinal lactic acid bacteria and bifidobacteria.to investigate the distribution of lactic acid bacteria (lab) inhabiting canine intestines, a total of 374 gram-positive lab and bifidobacteria (bf) isolated from large intestinal contents in 36 dogs were classified and identified by phenotypic and genetic analyses. based on cell morphological sizes, these isolates were divided into seven biotypes containing the genera lactobacillus, bifidobacterium, enterococcus, and streptococcus. the lab and bf isolates were classified into 38 chemotypes base ...200717951981
effect of bovicin hc5 on growth and spore germination of bacillus cereus and bacillus thuringiensis isolated from spoiled mango pulp.to use bovicin hc5 to inhibit predominant bacteria isolated from spoiled mango pulp.200717381743
improvement in lactic acid production from starch using alpha-amylase-secreting lactococcus lactis cells adapted to maltose or starch.to achieve direct and efficient lactic acid production from starch, a genetically modified lactococcus lactis il 1403 secreting alpha-amylase, which was obtained from streptococcus bovis 148, was constructed. using this strain, the fermentation of soluble starch was achieved, although its rate was far from efficient (0.09 g l(-1) h(-1) lactate). high-performance liquid chromatography revealed that maltose accumulated during fermentation, and this was thought to lead to inefficient fermentation. ...200717384945
streptococcus bovis biotype i meningoencephalitis in an alpaca (lama pacos) cria. 200717351176
geno- and phenotypic diversity of avian isolates of streptococcus gallolyticus subsp. gallolyticus (streptococcus bovis) and associated diagnostic problems.recently, strains of streptococcus bovis were reclassified as streptococcus gallolyticus. in the present study we describe for the first time an outbreak of s. gallolyticus in a broiler flock. mortality during the first week was normal (<1%), with a final total mortality at the end of production reaching 4.3%. specific symptoms were not observed. postmortem pathology demonstrated enlarged and light spleens and livers accompanied by multifocal irregular necroses surrounded by a hemorrhagic zone. ...200717166965
colon cancer presenting as streptococcus gallolyticus infective endocarditis.a 58-year-old chinese man presented with a three-week history of fever. he had a background history of rheumatic heart disease, hypertension, and thalassaemia. he was found to have infective endocarditis of the aortic valve due to streptococcus gallolyticus. during the hospital stay, he developed a few episodes of haematochaezia and was subsequently found to have colonic carcinomain- situ. he completed appropriate antibiotic treatment for his infective endocarditis and underwent a left hemicolec ...200717304377
nocardia brain abscesses in a male patient with sle: successful outcome despite delay in diagnosis.we report a 37-year-old african-american man with systemic lupus erythematosus (sle) diagnosed in may 2001 when he presented with biopsy-proven nephritis. he had been treated intermittently from may 2001 to november 2004 with intravenously (i.v.) administered cyclophosphamide and high doses of prednisone due to unrelenting proteinuria. in november 2004, he was admitted to the hospital because of deterioration of renal function and massive proteinuria (21 g dl(-1) 24 h(-1)) and treated with pulse ...200716683177
two coincidental neurological complications as a presenting form of infectious endocarditis.a 46-year-old man with two consecutive neurological events (an ischemic stroke and an intracerebral hemorrhage) is presented. streptococcus bovis biotype i was found in blood cultures and echocardiography showed native mitral valve mobile vegetations. the patient died due to the extension of intracerebral hemorrhage.200717360055
identification of an erm(t) gene in strains of inducibly clindamycin-resistant group b streptococcus.five inducibly clindamycin (cli)-resistant group b streptococcus (gbs) isolates, all negative for erm(a) and erm(b) genes, were found to contain erm(t), a gene previously reported in erythromycin-resistant animal isolates of lactobacillus spp. and human isolates of streptococcus bovis. one additional gbs isolate, constitutively resistant to cli, was also positive for the erm(t) gene in addition to erm(b). to our knowledge, this is the 1st report of erm(t) in gbs, the 2nd bacterial species from h ...200716989973
tigecycline (gar-936) activity against streptococcus gallolyticus (bovis) and viridans group streptococci.viridans group streptococci including streptococcus gallolyticus (formerly s. bovis) represent serious invasive pathogens often associated with endocarditis or sepsis among immunocompromised or cancer patients. tigecycline (gar-936), the first clinically studied glycylcycline, has a potent gram-positive activity with a potential treatment option for these streptococcal infections. the studied collection (848 strains) included 100 isolates each of streptococcus anginosus, streptococcus constellat ...200716989974
direct production of l-lysine from raw corn starch by corynebacterium glutamicum secreting streptococcus bovis alpha-amylase using cspb promoter and signal sequence.corynebacterium glutamicum is an important microorganism in the industrial production of amino acids. we engineered a strain of c. glutamicum that secretes alpha-amylase from streptococcus bovis 148 (amya) for the efficient utilization of raw starch. among the promoters and signal sequences tested, those of cspb from c. glutamicum possessed the highest expression level. the fusion gene was introduced into the homoserine dehydrogenase gene locus on the chromosome by homologous recombination. l-ly ...200717891388
fluorescence in situ hybridization analysis of hindgut bacteria associated with the development of equine laminitis.carbohydrate-induced laminitis in horses is characterized by marked changes in the composition of the hindgut microbiota, from a predominantly gram-negative population to one dominated by gram-positive bacteria. the objective of this study was to monitor changes in the relative abundance of selected hindgut bacteria that have previously been implicated in the pathophysiology of equine laminitis using fluorescence in situ hybridization (fish). caecal cannulae were surgically implanted in five sta ...200717635552
effect of a blend of essential oil compounds on the colonization of starch-rich substrates by bacteria in the rumen.to investigate the mode of action of a blend of essential oil compounds on the colonization of starch-rich substrates by rumen bacteria.200718045396
successful treatment of polymicrobial multivalve infective endocarditis. multivalve infective endocarditis.a 57-year-old man presented with triple valve infective endocarditis. there were vegetations on the tricuspid, mitral, and aortic valve. he had multiple complications such as pulmonary abscess, severe anaemia, and congestive heart failure. his general condition was extremely poor. intensive medical therapy, such as blood transfusion, mechanical ventilation, and inotropic support, allowed him to tolerate surgery. triple valve replacement was successfully performed without major complication. vege ...200717086366
steptococcus bovis meningitis and sepsis associated with strongyloidiasis in an immunocompetent patient.we report the case of a 69-year-old female who presented with headache, stiff neck, and decreased level of consciousness. lumbar puncture results were typical of bacterial meningitis. blood and cerebrospinal fluid cultures showed streptococcus bovis. subsequent serologic studies indicated concurrent strongyloides stercoralis infection, and larvae were visualized in two separate stool specimens. the patient responded to treatment of both infections. she refused to undergo colonoscopy despite a kn ...200718309863
first report of streptococcus bovis-associated acute cholecystitis in north america. 200818391832
infectious agents and colorectal cancer: a review of helicobacter pylori, streptococcus bovis, jc virus, and human papillomavirus.based on the high volume of bacteria and viruses that the intestine is exposed to and the importance of infectious agents in some gastrointestinal and anogenital cancers, it is not surprising the many studies have evaluated the association between colorectal cancer and infectious agents. this review highlights investigations of four agents in relation to colorectal cancer. helicobacter pylori, streptococcus bovis, jc virus, and human papillomavirus have all been evaluated as possible etiologic a ...200818990738
effects of feeding polyclonal antibody preparations on rumen fermentation patterns, performance, and carcass characteristics of feedlot steers.in a previous study, preparations of polyclonal antibodies (pap) against fusobacterium necrophorum (pap-fn) or streptococcus bovis (pap-sb) were successful in decreasing ruminal counts of target bacteria and increasing ruminal ph in steers fed high-grain diets. the objective of this study was to evaluate the effects of feeding pap-fn or pap-sb on performance, carcass characteristics, and ruminal fermentation variables of feedlot steers. in exp. 1, during 2 consecutive years, 226 or 192 angus and ...200818955541
a traditional sudanese fermented camel's milk product, gariss, as a habitat of streptococcus infantarius subsp. infantarius.samples of the traditional sudanese fermented camel's milk product gariss representing 9 different regions in sudan were microbiologically characterized using an integrated approach including phenotypic and genotypic methods. lactic acid bacteria [log(cfu/g)=7.76-8.66] and yeasts [log(cfu/g)=6.05-7.79] were detected in high numbers. a total of 180 lab isolates were identified of which 123 isolates were found to belong to the streptococcus bovis group. thirteen representative isolates were charac ...200818774196
current features of infective endocarditis in elderly patients: results of the international collaboration on endocarditis prospective cohort study.elderly patients are emerging as a population at high risk for infective endocarditis (ie). however, adequately sized prospective studies on the features of ie in elderly patients are lacking.200818955638
identification of streptococcus gallolyticus subsp. macedonicus as the etiological agent in a case of culture-negative multivalve infective endocarditis by 16s rdna pcr analysis of resected valvular tissue.today, pcr using broad-range primers is being used increasingly to detect pathogens from resected heart valves. herein is described the first case of multivalve infective endocarditis where 16s rdna pcr was used to detect a single pathogen from two affected valves in a 61-year-old man. triple heart valve replacement was required despite six weeks of appropriate antimicrobial therapy. the organism was confirmed as streptococcus gallolyticus subsp. macedonicus, a member of the 's. equinus/s. bovis ...200818980096
use of primers based on the heat shock protein genes hsp70, hsp40, and hsp10, for the detection of bovine mastitis pathogens streptococcus agalactiae, streptococcus uberis and streptococcus bovis.streptococcus agalactiae, streptococcus uberis, and streptococcus bovis are three of the major pathogens which cause mastitis in dairy herds. since conventional methods for the detection of these mastitis pathogens are laborious and time-consuming, rapid methods are needed. with an attempt to know if heat shock protein (hsp) genes other than hsp60 gene, could be used for pcr primer designing, in this study, we tried to design pcr primers based on the heat shock protein genes hsp70, hsp40, and hs ...200818602244
association between rural residency, group d streptococcal endocarditis and colon cancer? 200818093229
system using tandem repeats of the ca peptidoglycan-binding domain from lactococcus lactis for display of both n- and c-terminal fusions on cell surfaces of lactic acid bacteria.here, we established a system for displaying heterologous protein to the c terminus of the peptidoglycan-binding domain (ca domain) of acma (a major autolysin from lactococcus lactis). western blot and flow cytometric analyses revealed that the fusion proteins (ca-amya) of the ca domain and alpha-amylase from streptococcus bovis 148 (amya) are efficiently expressed and successfully displayed on the surfaces of l. lactis cells. amya was also displayed on the cell surface while retaining its activ ...200818156338
streptococcus henryi sp. nov. and streptococcus caballi sp. nov., isolated from the hindgut of horses with oligofructose-induced laminitis.four gram-positive, catalase-negative, coccoid-shaped isolates were obtained from the caecum and rectum of horses with oligofructose-induced equine laminitis. phenotypic and phylogenetic studies were performed on these isolates. initial biochemical profiling assigned two of the isolates to streptococcus bovis. the other two isolates, however, could not be assigned conclusively on the basis of their biochemical profiles. gene sequence analysis demonstrated that the four new isolates were related ...200818175719
bovicin hc5 reduces thermal resistance of alicyclobacillus acidoterrestris in acidic mango pulp.to test the effect of bovicin hc5 against vegetative cells and endospores of alicyclobacillus acidoterrestris dsmz 2498 in synthetic media and in acidic mango pulp.200818217925
[a case of bacterial meningitis due to streptococcus bovis in an infant with normal cerebrospinal fluid findings at the first csf examination].streptococcus bovis very occasionally causes rarely sepsis, endocarditis, and meningitis in newborns and the elderly. we report the case of infant meningitis caused by s. bovis despite normal cerebrospinal fluid (csf) findings at the first csf examination. a 77-day-old boy with 21-trisomy and patent foramen ovale and seen for a high fever underwent blood examination and lumbar puncture due to toxic appearance despite a lack of meningeal signs, and was admitted. his csf findings were normal and h ...200818306675
infective endocarditis from streptococcus viridans associated with colonic carcinoma: a case report.streptococcal bacteremia is an uncommon presentation for colorectal malignancy, yet most physicians would probably be aware of the association between streptococcus bovis infection and such malignancy; however, many physicians may be unaware that other streptococcal species are also associated with colon and rectal cancers. we describe a 62-year-old male with adenocarcinoma of the sigmoid colon with the presentation of infective endocarditis due to infection by streptococcus viridans, and we als ...200818435648
bioethanol production from uncooked raw starch by immobilized surface-engineered yeast cells.surface-engineered yeast saccharomyces cerevisiae codisplaying rhizopus oryzae glucoamylase and streptococcus bovis alpha-amylase on the cell surface was used for direct production of ethanol from uncooked raw starch. by using 50 g/l cells during batch fermentation, ethanol concentration could reach 53 g/l in 7 days. during repeated batch fermentation, the production of ethanol could be maintained for seven consecutive cycles. for cells immobilized in loofa sponge, the concentration of ethanol c ...200818425612
the activity and stability of cell-associated activity of bovicin hc5, a bacteriocin from streptococcus bovis hc5.streptococcus bovis hc5 cultures released a broad spectrum lantibiotic, bovicin hc5, into the cell-fee culture supernatant after they reached stationary phase, but most of the antibacterial activity remained cell-associated. cell-associated bovicin hc5 was more resistant to degradation by pronase e than the cell-free activity. acidic nacl (ph 2.0, 100 mm) did not release all of the cell-associated activity, and cells that were sequentially treated with acidic nacl and pronase e still had antibac ...200818445020
colonoscopy is mandatory after streptococcus bovis endocarditis: a lesson still not learned. case report.even though the relationship between certain bacterial infections and neoplastic lesions of the colon is well-recognized, this knowledge has not been sufficiently translated into routine practice yet.200818474093
new strategy for enhancement of microbial viability in simulated gastric conditions based on display of starch-binding domain on cell surface.the c-terminal region of the peptidoglycan hydrolase (cph) of lactococcus lactis il1403 fused to the linker region and the starch-binding domain (sbd) of the *-amylase of streptococcus bovis 148 was produced intracellularly in escherichia coli. the fusion protein (cph-sbd) was able to bind to the cell surface of lactobacillus casei nrrl b-441 and to corn starch. therefore, adhesion of cells to corn starch was mediated by the fusion protein. at a cell density of 10(9) cfu/ml and a starch concentr ...200818558341
molecular properties and transcriptional control of the phosphofructokinase and pyruvate kinase genes in a ruminal bacterium, streptococcus bovis.molecular properties of pyruvate kinase (pyk) and phosphofructokinase (pfk) in streptococcus bovis and transcriptional control of the two enzymes were examined. sequence analysis indicated that the pyk gene (pyk) clusters with the pfk gene (pfk) and several other genes. it was demonstrated that the pyk and pfk are cotranscribed and their transcription appeared to be regulated at the transcriptional level in response to the sugars supplied. the intracellular pyk-mrna level was lower in a cataboli ...200818565772
association of streptococcus bovis haematolgenous infection in total knee replacement with large intestine carcinoma. 200819292146
adherence characteristics of endocarditis-derived streptococcus gallolyticus ssp. gallolyticus (streptococcus bovis biotype i) isolates to host extracellular matrix proteins.members of the streptococcus bovis group are frequent colonizers of the intestinal tract, which can also cause endocarditis. however, their ability to adhere to and colonize host tissues and the factors associated with pathogenicity are largely unknown. here, we assessed 17 endocarditis-derived human isolates [identified here as 15 streptococcus gallolyticus ssp. gallolyticus (s. bovis biotype i), one s. gallolyticus ssp. pasteurianus (biotype ii/2) and one streptococcus infantarius ssp. coli (b ...200819054100
lactobacillus casei secreting alpha-msh induces the therapeutic effect on dss-induced acute colitis in balb/c mice.the neuropeptide alpha-melanocyte-stimulating hormone (alpha- msh) has anti-inflammatory property by downregulating the expressions of proinflammatory cytokines. because alpha-msh elicits the anti-inflammatory effect in various inflammatory disease models, we examined the therapeutic effect of oral administration of recombinant lactobacillus casei, which secretes alpha-msh (l. casei-alpha-msh), on dextran sulfate sodium (dss)-induced colitis in balb/c mice. thus, we constructed the alpha-msh-sec ...200819131702
the characterization of lactic acid producing bacteria from the rumen of dairy cattle grazing on improved pasture supplemented with wheat and barley grain.to identify and characterize the major lactic acid bacteria in the rumen of dairy cattle grazing improved pasture of rye grass and white clover and receiving a maize silage and grain supplement with and without virginiamycin.200818217928
[infectious endocarditis secondary to streptococcus bovis revealing adenomatous polyposis coli ]. 200819472746
[infectious endocarditis on probe of pace maker]. 200819476150
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