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procalcitonin levels in bloodstream infections caused by different sources and species of bacteria.the aim of this study was to evaluate procalcitonin (pct) diagnostic accuracy in discriminating gram-negative (gn) from gram-positive (gp) bloodstream infections and determining the relationship between pct levels, infection sites, and pathogen types.201727979420
non-infected penile prosthesis cultures during revision surgery; comparison between antibiotic coated and non - coated devices.aim of this study is to investigate bacterial growth on non-infected devices and compare antibiotic-coated and non-coated implants.201727622281
antimicrobials from human skin commensal bacteria protect against staphylococcus aureus and are deficient in atopic dermatitis.the microbiome can promote or disrupt human health by influencing both adaptive and innate immune functions. we tested whether bacteria that normally reside on human skin participate in host defense by killing staphylococcus aureus, a pathogen commonly found in patients with atopic dermatitis (ad) and an important factor that exacerbates this disease. high-throughput screening for antimicrobial activity against s. aureus was performed on isolates of coagulase-negative staphylococcus (cons) colle ...201728228596
[investigation of biofilm formation properties of staphylococcus isolates].biofilm production is an important virulence factor which allows staphylococci to adhere to medical devices. the principal component of biofilm is a "polysaccharide intercellular adhesin (pia)" which is composed of a beta-1,6-n-acetylglucosamine polymer synthesized by an enzyme (n-acetylglucosamine transferase) encoded by the ica operon found on the bacterial chromosome. this operon is composed of four genes (a, b, c, and d), and a transposable element is256. in this study, we aimed to determine ...201728283006
the lytic activity of recombinant phage lysin lyskδamidase against staphylococcal strains associated with bovine and human infections in the jiangsu province of china.we investigated the lytic activity of the bacteriophage endolysin (lysin) lyskδamidase against live methicillin-resistant and-susceptible staphylococcal strains clinically isolated from bovine milk and humans from different origins of china. antibiotic resistance patterns, multilocus sequence typing and sccmec type of 137 staphylococcal strains isolated from bovine milk associated with bovine mastitis and human diseases were studied. a lytic enzyme, lyskδamidase, was constructed by fusing the n- ...201728235706
coagulase-negative staphylococci in southern brazil: looking toward its high diversity.coagulase-negative staphylococci (cons) are the most prevalent pathogens in nosocomial infections and may serve as a reservoir of mobile genetic elements such as the staphylococcal cassette chromosome mec (sccmec) encoding methicillin resistance. molecular characterization of sccmec types combined with advanced molecular typing techniques may provide essential information for understanding the evolution and epidemiology of cons infections. we therefore aimed to investigate the sccmec distributio ...201727384825
draft genome sequence of staphylococcus hominis bhg17 isolated from wild bar-headed goose (anser indicus) feces.staphylococcus hominis belongs to a group of coagulase-negative staphylococci and is an opportunistic pathogen, usually found on the skin and mucous membranes. studies involving s. hominis isolated from wild birds are scarce. here, we report a 2.365-mb draft genome sequence of s. hominis bhg17, isolated from the feces of a bar-headed goose.201728153901
coagulase-negative staphylococci causing blood stream infection at an indian tertiary care hospital: prevalence, antimicrobial resistance and molecular characterisation.recent years have seen a rise of coagulase-negative staphylococci (cons) from common contaminants to agents of nosocomial blood stream infections (bsi's). molecular typing and establishing a correlation with antibiotic resistance is essential particularly in countries like india where genotyping studies for drug-resistant cons are sparse.201727934830
identification of emerging human mastitis pathogens by maldi-tof and assessment of their antibiotic resistance patterns.lactational mastitis constitutes one of the main causes of undesired weaning, depriving the mother-infant pair from the benefits of breastfeeding; therefore, this condition should be considered a relevant public health issue. the role of specific microorganisms remains unclear since human milk cultures and antibiotic susceptibility testing (ast) are not routinely performed, despite the fact that this would be key to ensure an early and effective diagnosis and treatment. the objective of this stu ...201728747897
microbiological and molecular identification of bacterial species isolated from nasal and oropharyngeal mucosa of fuel workers in riyadh, saudi arabia.this study aimed to determine the bacterial species colonizing the nasal and oropharyngeal mucosa of fuel workers in central riyadh, saudi arabia on a microbiological and molecular level. throat and nasal swab samples were obtained from 29 fuel station attendants in the period of time extending from march to may 2014 in riyadh, saudi arabia. microbiological identification techniques were utilized to identify the bacterial species isolated. antibiotic sensitivity was assessed for each of the bact ...201728855823
[distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015: results from a multicenter, retrospective study].objective: to describe the distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015 to provide reference for empirical anti-infection treatment. methods: pathogens were from hematology department of 26 tertiary hospitals in jiangsu province from 2014 to 2015. antimicrobial susceptibility testing was carried out according to a unified protocol using kirby-bauer method or agar dilution method. collection of drug susceptibility results and corresp ...201728810329
tetracycline resistance phenotypes and genotypes of coagulase-negative staphylococcal isolates from bubaline mastitis in egypt.this study was devoted to elucidate the tetracycline resistance of coagulase-negative staphylococci (cns) derived from normal and subclinical mastitic (scm) buffaloes' milk in egypt.201728717325
prebiotic effect of xylooligosaccharides produced from birchwood xylan by a novel fungal gh11 xylanase.a fungal endoxylanase belonging to the glycoside hydrolase gene family 11 (gh11) was obtained from the ascomycete talaromyces amestolkiae. the enzyme was purified, characterized and used to produce a mixture of xylooligosaccharides (xos) from birchwood xylan. a notable yield of neutral xos was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components. the prebiotic potential of this mixture ...201728490053
antimicrobial resistance and virulence characterization of staphylococcus aureus and coagulase-negative staphylococci from imported beef meat.the objectives of this study were to characterize the diversity and magnitude of antimicrobial resistance among staphylococcus species recovered from imported beef meat sold in the egyptian market and the potential mechanisms underlying the antimicrobial resistance phenotypes including harboring of resistance genes (meca, cfr, gyra, gyrb, and grla) and biofilm formation.201728486995
biofilm formation of methicillin-resistant coagulase negative staphylococci (mr-cons) isolated from community and hospital environments.methicillin-resistant coagulase negative staphylococci (mr-cons) are the major cause of infectious diseases because of their potential ability to form biofilm and colonize the community or hospital environments. this study was designed to investigate the biofilm producing ability, and the presence of meca, icaad, bap and fnba genes in mr-cons isolates. the mr-cons used in this study were isolated from various samples of community environment and five wards of hospital environments, using mannito ...201728859149
changing epidemiology of coagulase-negative staphylococci in normal flora of skin.coagulase negative staphylococci (cons) have recently emerged as important pathogens causing nosocomial blood stream infections. to evaluate the prevalence of cons in cutaneous normal flora, skin swabs were collected from voluntary blood donors and processed for culture and identification using matrix assisted laser desorption ionisation-time of flight (maldi-tof). cons were isolated from 96% of blood donors, most commonly staphylococcus hominis (86%), followed by staphylococcus epidermidis (22% ...201728681820
survival of bacteria in respiratory protective filters.the aim of this study was to estimate the susceptibility of staphylococcus hominis bacteria to the bacteriostatic agent in respiratory protective filters. four types of filter media of different characteristics were tested. the number of bacteria was estimated by a culture-based method. it was proved that in treated filters the number of s. hominis was significantly reduced, even below the detection limit, while in untreated material bacteria were able to grow and multiply up to 100-fold within ...201728735333
characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mec analysis of methicillin resistant staphylococcus hominis from blood cultures of children.methicillin resistant staphylococcus hominis (mrsho) has been recognized as an important human pathogen, particularly in immunocompromised patients.201728700050
whole-genome sequence of staphylococcus hominis strain j31 isolated from healthy human skin.we report here the first whole-genome sequence of a skin-associated strain of staphylococcus hominis determined using the pacbio long-read sequencing platform. s. hominis is a major commensal of the skin microflora. this genome sequence adds to our understanding of this species and will aid studies of gene traffic between staphylococci.201728408691
draft genome sequence of staphylococcus hominis strain hudgins isolated from human skin implicates metabolic versatility and several virulence determinants.staphylococcus hominis is a predominant member of the human skin microbiome. we here report on the genomic analysis of staphylococcus hominis strain hudgins that was isolated from the wrist area of human skin. the partial genome assembly of s. hominis hudgins consists of 2,211,863 bp of dna with 2174 protein-coding genes and 90 rna genes. based on the genomic analysis of kegg pathways, the organism is expected to be a versatile heterotroph potentially capable of hydrolyzing the sugars glucose, f ...201627766204
[ophthalmoplegia without severe painful eyelid swelling in acute dacryoadenitis: a case report].here, we present a case of right eyelid drooping in a 79-year-old man. neurological examination revealed ptosis of the right eye without severe painful eyelid swelling and redness. an ocular motility examination of the right eye revealed upward limitation and downward overshoot. the results of routine blood examinations were within normal limits, and no autoantibodies were detected. orbital magnetic resonance images revealed mild right eyelid swelling and lacrimal gland enlargement, indicating o ...201626616487
molecular basis of resistance to macrolides, lincosamides and streptogramins in staphylococcus hominis strains isolated from clinical specimens.coagulase-negative staphylococci (cons) are the most frequently isolated bacteria from the blood and the predominant cause of nosocomial infections. macrolides, lincosamides and streptogramin b (mlsb) antibiotics, especially erythromycin and clindamycin, are important therapeutic agents in the treatment of methicillin-resistant staphylococci infections. among cons, staphylococcus hominis represents the third most common organism. in spite of its clinical significance, very little is known about ...201626253583
genotypic diversity of methicillin-resistant coagulase-negative staphylococci isolated from inpatients and outpatients.we investigated the prevalence of methicillin-resistant coagulase-negative staphylococci (mrcons) isolated from hospitalized patients and outpatients (op). out of 350 staphylococcal isolates collected from three hospitals, 190 were coagulase-negative staphylococci (cons). these isolates were subjected to antimicrobial susceptibility tests, detection of meca, and pulsed-field gel electrophoresis (pfge) typing. among the 190 isolated cons, staphylococcus epidermidis (47.3%) and staphylococcus haem ...201626248114
short communication: prevalence, antimicrobial resistance, and resistant traits of coagulase-negative staphylococci isolated from cheese samples in turkey.a total of 17 coagulase-negative staphylococci (cns) isolates obtained from 72 cheese samples were included in this study. coagulase-negative staphylococci isolates obtained in this study comprised 6 (35.3%) staphylococcus saprophyticus, 3 (17.6%) staphylococcus epidermidis, 2 (11.8%) staphylococcus hominis, 2 (11.8%) staphylococcus haemolyticus, 1 (5.9%) staphylococcus xylosus, 1 (5.9%) staphylococcus vitulinus, 1 (5.9%) staphylococcus lentus, and 1 (5.9%) staphylococcus warneri. the disc diffu ...201626874419
characterization of staphylococci in urban wastewater treatment plants in spain, with detection of methicillin resistant staphylococcus aureus st398.the objective of this study was to determine the prevalence of staphylococcus in urban wastewater treatment plants (uwtp) of la rioja (spain), and to characterize de obtained isolates. 16 wastewater samples (8 influent, 8 effluent) of six uwtps were seeded on mannitol-salt-agar and oxacillin-resistance-screening-agar-base for staphylococci and methicillin-resistant staphylococcus aureus recovery. antimicrobial susceptibility profile was determined for 16 antibiotics and the presence of 35 antimi ...201626840519
bulb syringe and pulsed irrigation: which is more effective to remove bacteria in spine surgeries?retrospective study.201624346053
antimicrobial activity of ceftaroline and comparator agents when tested against numerous species of coagulase-negative staphylococcus causing infection in us hospitals.a total of 1593 coagulase-negative staphylococci (cons) considered clinically significant were collected from 71 us medical centers in 2013-2014 and tested for susceptibility by clsi broth microdilution methods. species identification was performed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. overall, 59.7% of isolates were oxacillin resistant (mrcons). ceftaroline (mic50/90, 0.25/0.5μg/ml) inhibited 99.2% of cons at ≤1μg/ml (susceptible breakpoint for staphyl ...201626971182
endophthalmitis caused by gram-positive organisms with reduced vancomycin susceptibility: literature review and options for treatment.endophthalmitis caused by gram-positive organisms with reduced vancomycin susceptibility and/or resistance is an important clinical issue worldwide.201626701686
new insight into microbial diversity and functions in traditional vietnamese alcoholic fermentation.the roles of microorganisms in traditional alcoholic fermentation are often assumed based on abundance in the starter and activity in pure culture. there is a serious lack of hard evidence on the behavior and activity of individual microbial species during the actual fermentation process. in this study, microbial succession and metabolite changes during 7days of traditional vietnamese alcoholic fermentation were monitored. special attention was devoted to starch degradation. in total, 22 microbi ...201627236224
immunostimulation by phospholipopeptide biosurfactant from staphylococcus hominis in oreochromis mossambicus.the immunostimulatory effect of phospholipopeptide biosurfactant from staphylococcus hominis (genbank accession no: kj564272) was assessed with oreochromis mossambicus. the non-specific (serum lysozyme activity, serum antiprotease activity, serum peroxidase activity and serum bactericidal activity), specific (bacterial agglutination assay) immune responses and disease resistance activity against aeromonas hydrophila were examined. fish were intraperitonially injected with water soluble secondary ...201626549172
conjunctival and intraocular swabs for the microbiological assessment of donor corneas.in this study, we investigated the associations between conjunctival (co) and intraocular (io) swabs and their implications for the contamination rates of organ-cultured corneas.201626146765
impact of plant extracts and antibiotics on biofilm formation of clinical isolates from otitis media.otitis media can lead to severe health consequences, and is the most common reason for antibiotic prescriptions and biofilm-mediated infections. however, the increased pattern of drug resistance in biofilm forming bacteria complicates the treatment of such infections.201627099692
anti-breast cancer effects of live, heat-killed and cytoplasmic fractions of enterococcus faecalis and staphylococcus hominis isolated from human breast milk.development of tumour that is resistant to chemotherapeutics and synthetic drugs, coupled with their life-threatening side effects and the adverse effects of surgery and hormone therapies, led to increased research on probiotics' anticancer potentials. the current study investigated the potential of live, heat-killed cells (hkc) and the cytoplasmic fractions (cf) of enterococcus faecalis and staphylococcus hominis as anti-breast cancer agents. mcf-7 cell line was treated with 25, 50, 100 and 200 ...201626659392
genome sequences of staphylococcus hominis strains shas1, shas2, and shas3, isolated from the asian malaria mosquito anopheles stephensi.staphylococcus hominis is a culturable component of the bacterial microbiome of anopheles stephensi. here, we present the annotated draft genome sequences of three s. hominis isolates from a. stephensi. these genomic resources will facilitate experiments to further our understanding of the role of bacteria in mosquito biology.201626966197
actinomyces gerencseriae hip prosthesis infection: a case report.actinomyces bacteria are part of the human oropharyngeal microbiota. they have been associated with abdominal, cervicofacial and thoracic infections and a few cases of joint infections have also been described. in particular, actinomyces gerencseriae, formerly described as actinomyces israelii serovar ii, has rarely been associated with human infections, mostly involving cervicofacial lesions and periodontal diseases. here, we report one case of hip prosthesis infection due to a. gerencseriae.201526415492
diversity of culturable bacterial endophytes of saffron in kashmir, india.saffron (crocus sativus) is a medicinally important plant. the kashmir valley (j&k, india) emblematizes one of the major and quality saffron producing areas in the world. nonetheless, the area has been experiencing a declining trend in the production of saffron during the last decade. poor disease management is one of the major reasons for declining saffron production in the area. endophytes are known to offer control against many diseases of host plant. during the present study, culturable bact ...201526558164
screening agars for mrsa: evaluation of a stepwise diagnostic approach with two different selective agars for the screening for methicillin-resistant staphylococcus aureus (mrsa).colonization with methicillin-resistant staphylococcus aureus (mrsa) poses a hygiene risk that does not spare field hospitals or military medical field camps during military deployments. diagnostic options for unambiguously identifying mrsa isolates are usually scarce in military environments. in this study, we assessed the stepwise application of two different selective agars for the specific identification of mrsa in screening analyses.201526199735
prevalence of resistant gram-negative bacilli in bloodstream infection in febrile neutropenia patients undergoing hematopoietic stem cell transplantation: a single center retrospective cohort study.bloodstream infection (bsi) is an important cause of morbidity and mortality in patients undergoing hematopoietic stem cell transplantation (hsct). to evaluate the causative bacteria and identify risk factors for bsi associated mortality in febrile neutropenia patients undergoing hsct, we collected the clinical and microbiological data from patients underwent hsct between 2008 and 2014 and performed a retrospective analysis. throughout the study period, among 348 episodes of neutropenic fever in ...201526559260
identification of axillary staphylococcus sp. involved in the production of the malodorous thioalcohol 3-methyl-3-sufanylhexan-1-ol.the production of malodour by humans is mediated by bacterial transformation of naturally secreted, non-odorous molecules. specifically in the underarm (axilla), malodour arises due to biotransformation by the microbiota of dipeptide-conjugated thioalcohols, particularly s-[1-(2-hydroxyethyl)-1-methylbutyl]-(l)-cysteinylglycine (cys-gly-3m3sh). this molecule, secreted by the axilla, has a well-established role in malodour when metabolized to free thioalcohol by bacteria. we present cys-gly-3m3sh ...201526163522
identification of abc transporter genes conferring combined pleuromutilin-lincosamide-streptogramin a resistance in bovine methicillin-resistant staphylococcus aureus and coagulase-negative staphylococci.the aim of this study was to investigate the genetic basis of combined pleuromutilin-lincosamide-streptogramin a resistance in 26 unrelated methicillin-resistant staphylococcus aureus (mrsa) and coagulase-negative staphylococci (cons) from dairy cows suffering from mastitis. the 26 pleuromutilin-resistant staphylococcal isolates were screened for the presence of the genes vga(a), vga(b), vga(c), vga(e), vga(e) variant, sal(a), vmlr, cfr, lsa(a), lsa(b), lsa(c), and lsa(e) by pcr. none of the 26 ...201525891423
skin commensal staphylococci may act as reservoir for fusidic acid resistance genes.we analyzed the occurrence and mechanisms of fusidic acid resistance present in staphylococci isolated from 59 healthy volunteers. the fingers of the volunteers were screened for the presence of staphylococci, and the collected isolates were tested for resistance to fusidic acid. a total of 34 fusidic acid resistant staphylococcal strains (all were coagulase-negative) were isolated from 22 individuals (22/59, 37.3%). examination of the resistance genes revealed that acquired fusb or fusc was pre ...201526581090
emergence of methicillin-resistant coagulase-negative staphylococci resistant to linezolid with rrna gene c2190t and g2603t mutations.the aim of this article were to determinate the mechanism of linezolid resistance in coagulase-negative methicillin-resistant staphylococci from hospitals in the northeast of brazil. we identified the isolates using vitek(®) 2 and maldi-tof. susceptibility to antibiotics was measured by the disk-diffusion method and by etest(®) . extraction of the whole genome dna was performed, followed by screening of all the strains for the presence of meca and cfr genes. the domain v region of 23s rrna gene ...201526227107
uncommon superficial angiomyxoma of the vulva complicated with condyloma acuminatum and staphylococcus hominis infection: a mere example of gynecological immunocompromised district. 201526227306
infectious ulcerative keratitis following retinopathy of prematurity treatment.to report the complication of infectious ulcerative keratitis after laser photocoagulation and pars plana vitrectomy (ppv) for retinopathy of prematurity (rop).201526065410
antibiotic susceptibility of biofilm cells and molecular characterisation of staphylococcus hominis isolates from blood.we aimed to characterise the staphylococcal cassette chromosome mec (sccmec) type, genetic relatedness, biofilm formation and composition, icaadbc genes detection, icad expression, and antibiotic susceptibility of planktonic and biofilm cells of staphylococcus hominis isolates from blood.201526659110
[linezolid resistant coagulase-negative staphylococcus: phenotypical and genotypical characteristics and sensitivity to antibiotic combinations].we recovered 22 coagulase-negative staphylococci isolates in our hospital to study their identity, susceptibility, epidemiological profile, linezolid resistance mechanisms, and the possibilities of different antibiotic combinations.201523260384
telavancin activity when tested by a revised susceptibility testing method against uncommonly isolated gram-positive pathogens responsible for documented infections in hospitals worldwide (2011-2013).the broth microdilution method for telavancin susceptibility testing was revised and now utilises dmso as solvent for stock solution preparation and diluent for stock solution dilution, following clsi guidelines for water-insoluble agents. the revised method also incorporates polysorbate 80 in the test medium to mitigate drug binding to plastics. this revised methodology provides more accurate and reproducible mic determinations, which results in values lower than the previously established meth ...201527873648
endophthalmitis caused by staphylococcus hominis and two different colonies of staphylococcus haemolyticus after cataract surgery.to describe a case of endophthalmitis after cataract surgery caused by staphylococcus hominis and two separate colony types of staphylococcus haemolyticus.201525764314
biofilm formation by staphylococcus hominis strains isolated from human clinical specimens.staphylococcus hominis is the third species of coagulase-negative staphylococci (cons) most frequently isolated from specimens of patients with hospital-acquired infections. many infections caused by cons appeared to be associated with biofilms. nevertheless, the knowledge of the ability of s. hominis to form a biofilm is limited. the aim of this study was to analyze the formation of the biofilm by 56 s. hominis strains isolated from clinical cases. the biofilm three-dimensional structure was re ...201524984828
multidrug-resistant staphylococcus hominis subsp. novobiosepticus causing septicemia in patients with malignancy.a new subspecies of staphylococcus hominis described by kloos et al. in 1998 and named s. hominis subsp. novobiosepticus (shn) has been implicated in nosocomial outbreaks. multidrug resistance, including resistance to novobiocin and oxacillin, is a particularly important feature of shn. in our institute, we encountered 13 cases of s. hominis subsp. hominis in cancer patients with septicemia, of which seven were methicillin resistant. the isolates were identified by vitek ® 2 compact automated sy ...201424943764
[infections of central venous catheters in home parenteral nutrition: a retrospective monocentric study over 2 years].infection of the central venous catheter (cvc) is the main aggravating factor of parenteral nutrition. the aim of this study was to determine the ecology of these infections in our home parenteral nutrition center and to evaluate our care protocol.201424768350
linezolid-resistant staphylococcus haemolyticus and staphylococcus hominis: single and double mutations at the domain v of 23s rrna among isolates from a rio de janeiro hospital.in this work, the molecular and phenotypic antimicrobial resistance and clonal diversity of 10 linezolid-resistant staphylococcus spp. isolates were investigated. the 7 staphylococcus haemolyticus isolates presented staphylococcal cassete chromosome mec (sccmec) v and belonged to the same pulsed-field gel electrophoresis pulsotype. their mics for oxacillin, vancomycin, and linezolid were ≥ 256 μg/ml, 1-4 μg/ml, and 8-16 μg/ml, respectively. the 3 s. hominis presented mic values 32 to >256 μg/ml, ...201425294302
clonal analysis of staphylococcus hominis strains isolated from hospitalized patients.staphylococcus hominis is a part of normal skin flora, but it is also a cause of nosocomial infections. the aim of this study was to investigate the genetic relatedness of 62 strains of s. hominis obtained from hospitalised patients during an 11-year period. for the discrimination of these clinical strains we used repetitive sequence-based pcr method (box-pcr) and multiple-locus variable-number tandem repeat analysis (mlva). box-pcr analysis revealed a large genetic diversity among clinical stra ...201425546946
chronic staphylococcus hominis endophthalmitis following injury with a retained intraocular foreign body. 201425190536
superficial angiomyxoma of the vulva complicated with condyloma acuminatum and staphylococcus hominis infection. 201423331125
do the pens used by nursing students in clinics cause bacterial contamination?health professionals and their medical equipment have long been known to act as vectors of infectious diseases. the aim of the present study was to evaluate the role of pens used by nursing students working in clinics in relation to transmission of bacteria.201424949075
comparison of sensititre microdilution method to other standard methods for susceptibility testing of coagulase-negative staphylococci from paediatric blood cultures.susceptibility testing on 60 isolates of staphylococcus epidermidis and 20 isolates of staphylococcus hominis was performed using the sensititre (trek diagnostics, cleveland, oh, usa) method and compared with vitek-2 automated susceptibility testing (biomèrieux, marcy l'etoile, france), mice strips for minimum inhibitory concentration determination (oxoid, basingstoke, uk), and disc diffusion (mast, bootle, uk). the categorical agreement between vitek and sensititre was greater than 90% for agen ...201424359933
three proposed new bacteriophage genera of staphylococcal phages: "3alikevirus", "77likevirus" and "phietalikevirus".to date, most members of the siphoviridae family of bacteriophages remain unclassified, including the 46 staphylococcal phages for which the complete genome sequences have been deposited in public databases. comparative nucleotide and protein sequence analysis, in addition to available data on phage morphology, allowed us to propose three new phage genera within the family siphoviridae: "3alikevirus", "77likevirus" and "phietalikevirus", which include related phages infecting staphylococcus aure ...201424022640
reliability of the microscan walkaway pc21 panel in identifying and detecting oxacillin resistance in clinical coagulase-negative staphylococci strains.the purpose of this study was to determine the reliability of the microscan walkaway poscombo21 (pc21) system for the identification of coagulase-negative staphylococci (cns) strains and the detection of oxacillin resistance. using molecular and phenotypic methods, 196 clinical strains were evaluated. the automated system demonstrated 100 % reliability for the identification of the clinical strains staphylococcus haemolyticus, staphylococcus hominis and staphylococcus cohnii; 98.03 % reliability ...201423887612
bacterial decolorization of textile azo dye acid orange by staphylococcus hominis rmlrt03.a bacterial strain rmlrt03 with ability to decolorize textile dye acid orange dye was isolated from textile effluent contaminated soil of tanda, ambedkar nagar, uttar pradesh (india). the decolorization studies were performed in bushnell and haas medium (bhm) amended with acid orange dye. the bacterial strain was identified as staphylococcus hominis on the basis of 16s rdna sequence. the bacterial strain exhibited good decolorization ability with glucose and yeast extract supplementation as cosu ...201425253925
performance of vitek 2 for antimicrobial susceptibility testing of staphylococcus spp. and enterococcus spp.vitek 2 (biomérieux, inc., durham, nc) is a widely used commercial antimicrobial susceptibility testing system. we compared mic results obtained by vitek 2 to those obtained by the clinical and laboratory standards institute (clsi) broth microdilution (bmd) reference method for 134 staphylococcal and 84 enterococcal clinical isolates. nineteen agents were evaluated, including all those available on vitek 2 for testing staphylococci and enterococci. the resistance phenotypes tested included methi ...201424478467
bacterial bloodstream infections and antimicrobial susceptibility pattern in pediatric hematology/oncology patients after anticancer chemotherapy.bloodstream infections in pediatric hematology and oncology represent a major problem worldwide, but this has not been studied in qatar. in this study, we investigated the burden of infection and the resistance pattern in the bacterial etiology, in the only tertiary pediatric hematology and oncology center in qatar.201425395866
the predominant bacteria isolated from radicular cysts.to detect predominant bacteria associated with radicular cysts and discuss in light of the literature.201324011184
internalisation potential of escherichia coli o157:h7, listeria monocytogenes, salmonella enterica subsp. enterica serovar typhimurium and staphylococcus aureus in lettuce seedlings and mature plants.the internalisation potential of listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7 and salmonella enterica subsp. enterica serovar typhimurium in lettuce was evaluated using seedlings grown in vermiculite in seedling trays as well as hydroponically grown lettuce. sterile distilled water was spiked with one of the four human pathogenic bacteria (10(5) cfu/ml) and used to irrigate the plants. the potential for pathogen internalisation was investigated over time using light mi ...201323708570
sonication of explanted cardiac implants improves microbial detection in cardiac device infections.the sonication technique has been shown to be a promising tool for microbiological diagnosis of device-related infections. we evaluated the usefulness of the sonication method for pathogen detection in 80 explanted cardiac components collected from 40 patients, and the results were compared with those of conventional cultures. forty subjects undergoing cardiac device removal were studied: 20 had cardiac device infection, and 20 subjects underwent elective generator replacement or revision in the ...201323196364
phylogeny of bacteria isolated from rhabditis sp. (nematoda) and identification of novel entomopathogenic serratia marcescens strains.twenty-five bacterial strains isolated from entomopathogenic nematodes were characterized to the genus level by 16s rrna phylogeny and blast analyses. bacteria strains isolated could be affiliated with seven genera. microbacterium-like isolates phylogenetically affiliated with m. oxydans while those of serratia were highly similar to s. marcescens. 16s rrna sequences of bacillus isolates matched those of both b. mycoides and b. weihenstephanesis. one isolate each matched pseudomonas mosselii, rh ...201323079959
normalization of creatine kinase values in a case of rhabdomyolysis during daptomycin treatment.a 41-year-old woman presented in the emergency department with suspected compartment syndrome of lower left leg (creatine kinase [ck]: 12,502 iu/l, cr: 4.31 mg/dl). fasciotomy of the four limb compartments was conducted. by day 2, the patient presented oliguria during previous 24 h, so daily intermittent dialysis was carried out. on day 12, the patient presented an episode of bacteremia due to staphylococcus hominis. treatment with vancomycin was initiated and was changed after 4 days to daptomy ...201323716901
antibiotic resistance in sepsis patients: evaluation and recommendation of antibiotic use.the appropriate selection of empirical antibiotics based on the pattern of local antibiotic resistance can reduce the mortality rate and increase the rational use of antibiotics.201323923107
prevalence and antimicrobial susceptibility patterns of bacteria from milkmen and cows with clinical mastitis in and around kampala, uganda.identification of pathogens associated with bovine mastitis is helpful in treatment and management decisions. however, such data from sub-saharan africa is scarce. here we describe the distribution and antimicrobial susceptibility patterns of bacteria from cows with clinical mastitis in kampala, uganda. due to high concern of zoonotic infections, isolates from milkmen are also described.201323667611
comparison of antibiotic resistance, biofilm formation and conjugative transfer of staphylococcus and enterococcus isolates from international space station and antarctic research station concordia.the international space station (iss) and the antarctic research station concordia are confined and isolated habitats in extreme and hostile environments. the human and habitat microflora can alter due to the special environmental conditions resulting in microbial contamination and health risk for the crew. in this study, 29 isolates from the iss and 55 from the antarctic research station concordia belonging to the genera staphylococcus and enterococcus were investigated. resistance to one or mo ...201323411852
activity of oritavancin against methicillin-resistant staphylococci, vancomycin-resistant enterococci and β-haemolytic streptococci collected from western european countries in 2011.to determine the activity of oritavancin against methicillin-resistant staphylococci, vancomycin-resistant enterococci (vre) and β-haemolytic streptococci recently isolated from acute bacterial skin and skin structure infections or bacteraemia in western europe.201322941898
[molecular cloning and characterization of a n-acetylneuraminate lyase gene from staphylococcus hominis].a n-acetylneuraminate lyase gene (shnal) from staphylococcus hominis was cloned into pet-28a and expressed in escherichia coli bl21 (de3) host cells. the recombinant enzyme was purified and characterized. it is a homotetrameric enzyme with the optimum ph at 8.0 for the cleavage direction and the optimum ph and temperature were 7.5 and 45 degrees c for the synthetic direction. the activity of shnal is stable when incubated at 45 degrees c for 2 h but decreased rapidly over 50 degrees c. shnal sho ...201323894821
bacterial flora and antimicrobial resistance in raw frozen cultured seafood imported to denmark.intensified aquaculture includes the use of antimicrobials for disease control. in contrast to the situation in livestock, escherichia coli and enterococci are not part of the normal gastrointestinal flora of fish and shrimp and therefore not suitable indicators of antimicrobial resistance in seafood. in this study, the diversity and phenotypic characteristics of the bacterial flora in raw frozen cultured and wild-caught shrimp and fish were evaluated to identify potential indicators of antimicr ...201323462087
silver/chitosan/cellulose fibers foam composites: from synthesis to antibacterial properties.chitosan, associated with cellulose fibers, can be used for elaborating sponge-like structures (membranes, foams) for the binding of silver ions. the composite material has very promising antibacterial properties versus pseudomonas aeruginosa (gram(-))≫escherichia coli (gram(-))>staphylococcus hominis (gram(+))≫staphylococcus aureus (gram(+)). the amount of silver required for bactericidal effect is quite low (below 0.1 mg per disk, this means less than 6 mg ag g(-1)) in antibiogram-type test bu ...201323245882
cardiovascular implantable electronic device endocarditis treated with daptomycin with or without transvenous removal.nine patients with cardiovascular implantable electronic device (cied) endocarditis were treated with daptomycin after the failure of previous treatment. the blood and cied lead cultures of 1 patient were negative. in the other 8 patients, we observed 6 monomicrobic infections and 2 polymicrobic infections. overall, 10 strains were isolated in these patients: 4 methicillin-sensitive staphylococcus aureus, 2 methicillin-sensitive staphylococcus epidermidis, 1 methicillin-resistant staphylococcus ...201322436967
synthesis, antibacterial activity and docking of 14-membered 9-o-(3-arylalkyl) oxime 11,12-cyclic carbonate ketolides.a series of 9-o-(3-aryl-2-propargyl)oxime ketolides 8 was synthesized and evaluated for in vitro antibacterial activity. among 8, 8b-8d, and 8h-8l displayed dramatically improved potency against inducibly mls(b)-resistant and efflux-resistant pathogens as compared to clarithromycin and azithromycin. especially, 8i (ar=4-isoquinolyl) possessed an mic of 0.064 μg/ml against constitutively mls(b)-resistant streptococcus pneumoniae, and mics of 0.032-0.064 μg/ml against methicillin-resistant staphyl ...201323202851
reactive oxygen species and the bacteriostatic and bactericidal effects of isoconazole nitrate.bacterial superinfections often occur in dermatomycoses, resulting in greatly inflamed or eczematous skin. the objective of this study was to evaluate the antibacterial efficacy of isoconazole nitrate (isn), a broad-spectrum antimicrobial imidazole, commonly used to treat dermatomycoses. several gram-positive bacteria minimal inhibitory concentrations (mics) for isn (isn solution or isn-containing creams: travogen or corticosteroid-containing travocort) and ampicillin were obtained using the bro ...201323574020
microbiological and molecular characterization of staphylococcus hominis isolates from blood.among coagulase-negative staphylococci (cons), staphylococcus hominis represents the third most common organism recoverable from the blood of immunocompromised patients. the aim of this study was to characterize biofilm formation, antibiotic resistance, define the sccmec (staphylococcal chromosomal cassette mec) type, and genetic relatedness of clinical s. hominis isolates.201323585877
emergence of cfr-harbouring coagulase-negative staphylococci among patients receiving linezolid therapy in two hospitals in china.this study reports on the emergence of cfr-harbouring coagulase-negative staphylococci (cons) among patients who received linezolid therapy in two hospitals in hangzhou, china. the mechanisms of resistance and transmission were analysed for these resistant isolates. eight staphylococcus capitis isolates, one staphylococcus epidermidis isolate and one staphylococcus hominis isolate, obtained from patients who had received linezolid therapy in two hospitals in hangzhou, china, were confirmed as li ...201323449871
clinical and molecular features of methicillin-resistant, coagulase-negative staphylococci of pets and horses.to determine the antibiotic resistance and fingerprint profiles of methicillin-resistant coagulase-negative staphylococci (mrcons) from animal infections among different practices and examine the history of antibiotic treatment.201323425780
intracranial calcification caused by a brain abscess : a rare cause of intracranial calcification.intracranial calcifications are relatively common computed tomographic findings in the field of neurosurgery, and cysticercosis, tuberculosis, hiv, and cryptococcus are acquired intracranial infections typically associated with calcifications. however, intracranial calcification caused by a bacterial brain abscess is rare. here, we present a rare case of intracranial calcification caused by a bacterial brain abscess, from which staphylococcus hominis was isolated. to the best of our knowledge, n ...201324175034
identification of compounds inhibiting the c-s lyase activity of a cell extract from a staphylococcus sp. isolated from human skin.the c-s lyase activity of bacteria in the human armpit releases highly malodorous, volatile sulfur compounds from nonvolatile precursor molecules. such compounds significantly contribute to human body odour. hence, c-s lyase represents an attractive target for anti-body-odour cosmetic products. here, aiming at a final use in an ethanol-based deodorant formulation, 267 compounds and compound mixtures were screened for their ability to inhibit the c-s lyase activity of a stapyhlococcus sp. crude e ...201323941521
genetic relatedness of coagulase-negative staphylococci from gastrointestinal tract and blood of preterm neonates with late-onset sepsis.coagulase-negative staphylococci (cons) are the first colonizers of gastrointestinal tract (git) and the commonest cause of late-onset sepsis (los) in preterm neonates. intravascular catheters are considered a major source of cons bacteremia. however, several cases of los remain without an identified source. to elucidate whether git could be a potential source of invasive strains, we aimed to assess the molecular similarity between cons from blood and git in preterm neonates with los.201323080292
breast milk and gut microbiota in african mothers and infants from an area of high hiv prevalence.human milk and infant gut microbiota are essential for the immune system maturation and protection against infections. there is scarce information on the microbiological composition of breast milk in general, and none from developing countries. the objective of the study was to characterize the breast milk and gut microbiota from mothers and infants from southern mozambique, where infections and breastfeeding are prevalent.201324303004
coagulase-negative staphylococci strains resistant to oxacillin isolated from neonatal blood cultures.coagulase-negative staphylococci (cons) are the microorganisms most frequently isolated from clinical samples and are commonly found in neonatal blood cultures. oxacillin is an alternative treatment of choice for cons infections; however, resistance to oxacillin can have a substantial impact on healthcare by adversely affecting morbidity and mortality. the objective of this study was to detect and characterise oxacillin-resistant cons strains in blood cultures of newborns hospitalised at the neo ...201324141968
[right ventricular mural endocarditis: description of a case].we report the case of a 45-year-old man addicted to intravenous drug abuse who was admitted to our hospital for dyspnea, fever and chest pain. chest x-ray showed diffuse right lung opacity and pleural effusion. transthoracic echocardiography and contrast-enhanced cardiac magnetic resonance imaging revealed a plurilobated, highly mobile mass in the right ventricle originating from the moderator band near the apical trabeculae. cardiac structure and valves were normal. blood cultures were positive ...201324336599
staphylococcus petrasii sp. nov. including s. petrasii subsp. petrasii subsp. nov. and s. petrasii subsp. croceilyticus subsp. nov., isolated from human clinical specimens and human ear infections.thirteen coagulase-negative, oxidase-negative, and novobiocin-susceptible staphylococci were isolated from human clinical specimens. the isolates were differentiated from known staphylococcal species on the basis of 16s rrna, hsp60, rpob, dnaj, tuf, and gap gene sequencing, automated ribotyping, (gtg)5-pcr fingerprinting, and maldi-tof ms analysis. phylogenetic analysis based on the 16s rrna gene sequence indicated phylogenetic relatedness of the analyzed strains to staphylococcus haemolyticus, ...201323318093
vancomycin-resistant staphylococcus hominis endophthalmitis following cataract surgery.we report a case of acute postoperative endophthalmitis caused by vancomycin-resistant staphylococcus hominis, treated at our hospital. an 80-year-old male presented 2 days after uncomplicated phacoemulsification and posterior chamber intraocular lens implantation, with a 24-hour history of progressive visual loss and redness in the operated (right) eye. on examination, best corrected visual acuity was counting fingers. anterior segment examination revealed conjunctival injection, chemosis, corn ...201323818754
multilocus sequence typing and further genetic characterization of the enigmatic pathogen, staphylococcus hominis.staphylococcus hominis is a commensal resident of human skin and an opportunistic pathogen. the species is subdivided into two subspecies, s. hominis subsp. hominis and s. hominis subsp. novobiosepticus, which are difficult to distinguish. to investigate the evolution and epidemiology of s. hominis, a total of 108 isolates collected from 10 countries over 40 years were characterized by classical phenotypic methods and genetic methods. one nonsynonymous mutation in gyrb, scored with a novel snp t ...201323776678
staphylococcus jettensis sp. nov., a coagulase-negative staphylococcal species isolated from human clinical specimens.eight coagulase-negative, novobiocin-susceptible staphylococcal strains were isolated from human clinical specimens at two different belgian medical facilities. all strains were non-motile, gram-stain-positive, catalase-positive cocci. dna g+c content, peptidoglycan type, menaquinone pattern, the presence of teichoic acid and cellular fatty acid composition were in agreement with the characteristics of species of the genus staphylococcus. sequencing of the 16s rrna gene and four housekeeping gen ...201323456811
linezolid resistance in brazilian staphylococcus hominis strains is associated with l3 and 23s rrna ribosomal mutations. 201323689714
whole-genome sequence of staphylococcus hominis, an opportunistic pathogen.staphylococcus hominis is a commensal coagulase-negative species of staphylococci. it has been considered a presumptive and opportunistic pathogen that causes nosocomial infections in humans. here we present the draft genome sequence of s. hominis zbw5, a multidrug-resistant strain isolated from a human skin sample, which provides opportunities to understand the mechanism and genetic basis of its pathogenesis.201222887665
co-infection by two linezolid-resistant coagulase-negative staphylococci with two different resistance determinants.linezolid resistance among gram-positive pathogens is being reported with increasing frequency. we examined 14 linezolid-resistant coagulase-negative staphylococci (cons) isolated from blood cultures obtained from patients admitted to the intensive care unit of vicenza general hospital, italy. the species identification yielded 10 staphylococcus epidermidis, 3 staphylococcus hominis, and 1 staphylococcus capitis. minimal inhibitory concentrations of linezolid ranged between 16 and 32 mg/l. by se ...201222830638
characterization of novel phages isolated in coagulase-negative staphylococci reveals evolutionary relationships with staphylococcus aureus phages.despite increasing interest in coagulase-negative staphylococci (cons), little information is available about their bacteriophages. we isolated and sequenced three novel temperate siphoviridae phages (stb12, stb27, and stb20) from the cons staphylococcus hominis and s. capitis species. the genome sizes are around 40 kb, and open reading frames (orfs) are arranged in functional modules encoding lysogeny, dna metabolism, morphology, and cell lysis. bioinformatics analysis allowed us to assign a po ...201222923589
species and staphylococcal cassette chromosome mec (sccmec) diversity among methicillin-resistant non-staphylococcus aureus staphylococci isolated from pigs.while methicillin-resistant staphylococcus aureus (mrsa) st398 is known to be widespread in pig farms, few studies have investigated the species diversity and sccmec types of methicillin-resistant non-s. aureus staphylococci (mrnas) residing in the nose of pigs. we examined nasal swab samples of 200 pigs originating from 10 belgian pig farms previously found positive for mrsa st398. suspected staphylococcal isolates were subjected to a 16s rrna-meca-nuc pcr. confirmed mrnas were genotypically id ...201222333287
spontaneous cholecystocutaneous fistula draining from an abdominal scar from previous surgical drainage.we present a rare case of cholecystocutaneous fistula draining from an old surgical scar in the right upper abdominal quadrant following chronic calculous cholecystitis. a 71 year old male presented to the emergency department with a persistent bilious drainage from an old surgical scare, from surgical drainage, of the right upper abdominal quadrant for about a week. cultures from the draining fluid grew staphylococcus hominis, escherichia coli and klebsilla pneumoniae and tigecycline 50 mg twic ...201222352221
antimicrobial activity of two techniques for arm skin disinfection of blood donors in brazil.evaluation of the antimicrobial effect of skin disinfection techniques is essential to avoid the transmission of infectious agents during blood transfusion. the aim of this study was to examine the effectiveness of two methods of arm skin disinfection used in blood donors at a hemotherapy center in brazil that represents an important centre for distributing haemocomponents to many cities in the country.201222283663
daptomycin lock therapy for grampositive long-term catheter-related bloodstream infections.to evaluate the efficacy of daptomycin (dpt) lock therapy in the treatment of grampositive long-term catheter-related bloodstream infections (lt-crbi).201222340450
zaaps program results for 2010: an activity and spectrum analysis of linezolid using clinical isolates from 75 medical centres in 24 countries.the zyvox® annual appraisal of potency and spectrum (zaaps) program monitors the in vitro activities of linezolid and comparator agents for gram-positive organisms in latin america, europe, canada, and the asia-pacific. for the 2010 program a total of 6305 gram-positive strains were collected from 75 medical centres on five continents (24 countries). reference broth microdilution susceptibility tests were performed on organisms from the following groups: staphylococcus aureus (2875), coagulase n ...201223174097
evolution of nasal carriage of methicillin-resistant coagulase-negative staphylococci in a remote population.nasal carriage of methicillin-resistant coagulase-negative staphylococci (mr-cons) is highly prevalent in community subjects, but its dynamic has been little investigated. nasal swabbing was performed in 2006 and 2008 in 154 amerindians living isolated in french guiana. mr-cons strains were identified and characterized by non-β-lactam susceptibility testing and staphylococcal cassette chromosome mec element (sccmec) typing, characterizing the associations of ccr and mec gene complex allotypes, a ...201222064532
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