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prevalence and persistence of coagulase-negative staphylococcus species in three dairy research herds.coagulase-negative staphylococcus species (cns) were isolated from 11.3% (1407 of 12,412) of mammary quarter milk samples obtained from cows in three dairy research herds in 2005. approximately 27% (383/1407) of cns was identified to the species level. the species distribution among those cns identified from all herds was staphylococcus chromogenes (48%), staphylococcus hyicus (26%), staphylococcus epidermidis (10%), staphylococcus simulans (7%), staphylococcus warneri (2%), staphylococcus homin ...200918950962
staphylococcus pseudintermedius versus staphylococcus intermedius. 200918760884
molecular diagnostic identification of staphylococcus pseudintermedius.we report the first diagnostic test for the identification of staphylococcus pseudintermedius involving a simple pcr-restriction fragment length polymorphism approach. the method allows discrimination of s. pseudintermedius from the closely related members of the staphylococcus intermedius group and other important staphylococcal pathogens of humans and dogs.200919091817
interventions for control of salmonella: clearance of microbial growth from rubber picker fingers.a study was conducted to determine if a surface material with antimicrobial properties combined with an effective disinfectant could achieve total clearance of bacterial contamination. before beginning the project, new rubber picker fingers collected from 3 processing facilities were tested for endogenous microflora. five species of bacteria common to soil and human handling were present: bacillus amyloliquefaciens, bacillus cereus/thuringiensis, staphylococcus epidermidis, staphylococcus homini ...200919439642
management of human and animal bite wound infection: an overview.animal and human bite wounds can lead to serious infections. the organisms recovered generally originate from the biter's oral cavity and the victim's skin flora. anaerobes were isolated from more than two thirds of human and animal bite infections. streptococcus pyogenes is often recovered in human bites, pasteurella multocida in animal bites, eikenella corrodens in animal and human, capnocytophaga spp, neisseria weaveri, weeksella zoohelcum, neisseria canis, staphylococcus intermedius, nonoxid ...200919698283
staphylococcal colonization of mucosal and lesional skin sites in atopic and healthy dogs.staphylococcal colonization was compared in healthy dogs and in dogs with atopic dermatitis. bacterial swabs were collected from the nasal mucosa, ear and perineum of 43 healthy and 24 atopic dogs and also from potentially infected skin lesions of the atopic dogs. coagulase positive staphylococcal isolates were identified to the species level. at the time of this study staphylococcus intermedius was considered a single species but has since been recognized as comprising at least three species wi ...200919392768
antibiotic resistance patterns and occurrence of meca gene in staphylococcus intermedius strains of canine origin.we have evaluated 102 staphylococcus intermedius isolates of canine origin for susceptibility to antimicrobial primary agents, i.e. penicillin, amoxicillin, amoxicillin with clavulanic acid, cefuroxime, trimethoprim/sulfonamides, neomycin, streptomycin, gentamicin, norfloxacin, tetracycline, vancomycin, erythromycin and secondary agents, i.e., chloramphenicol, ciprofloxacin, lincomycin, teicoplanin, rifampicin, imipenem, mupirocin. antimicrobial sensitivity was examined using the disk diffusion ...200919459434
evaluation of mucosal and seborrheic sites for staphylococci in two populations of captive psittacines.to survey 2 populations of psittacines to characterize staphylococcus spp isolated from commensal cutaneous microflora.200919335240
antimicrobial susceptibility of staphylococci isolated from naturally occurring canine external ocular diseases.the purpose of this study was to describe the species distribution and antimicrobial susceptibility patterns of staphylococci isolated from naturally occurring canine ocular diseases. samples and procedures: samples obtained from 68 dogs with signs of external eye disease were processed for isolation of bacteria of the genus staphylococcus sp. isolates were identified by biochemical reactions and tested for susceptibility to 11 antimicrobials of six classes.200919604336
central nervous system infection with staphylococcus intermedius secondary to retrobulbar abscessation in a dog.in this report, we describe a case of retrobulbar abscessation in a dog that was initially diagnosed as masticatory myositis and treated with immunosuppressive doses of corticosteroids. secondary bacterial infection of the central nervous system (cns) occurred and was definitively diagnosed by the analysis and culture of the cerebrospinal fluid. this is the first time that retrobulbar infection has been definitively shown to result in secondary bacterial infection of the cns in the dog and highl ...200919751495
meca gene transferrability and antibiogram of zoonotic staphylococcus intermedius from animals, staff and the environment in animal hospitals in korea.staphylococcus intermedius is a common cause of otitis externa, pyoderma and wound infections in companion animals. although s. intermedius infections are rare in humans, it is zoonotic, with several case reports describing fatal human infections. presently, we sought to isolate s. intermedius strains from various sources at animal hospitals nationwide in korea, examine their antibiotic susceptibilities, and determine the possibility of horizontal transmission between animals and humans. pulsed ...201020208451
evaluation of otoscope cone disinfection techniques and contamination level in small animal private practice.the objective of this study was to evaluate the level of bacterial contamination of otoscope cones in veterinary private practice, and to determine the most effective method of disinfection. fifty small animal practices participated in this study, which included a detailed survey regarding otoscope cleaning, storage and usage and quantitative culture of the cleaned and stored otoscope cones. using sterile technique, two cones from each of the 50 hospitals were swabbed and submitted for quantitat ...201020230588
evaluation of methicillin resistance in staphylococcus intermedius isolated from dogs.methicillin and multi-drug resistance were investigated in 136 staphylococcus intermedius strains of canine origin. the large majority of isolates were found to be meca-negative by polymerase chain reaction, whereas only four strains were positive for the meca gene. all meca-positive strains were confirmed as methicillin-resistant by complementary tests, except for oxacillin disk diffusion, which yielded one false-negative result. a significantly higher resistance to fusidic acid, lincosamides, ...201020437274
identification and antimicrobial susceptibility patterns of bacteria causing otitis externa in dogs.bacterial agents are considered important pathogens causing external otitis in dogs. it is essential to carry out bacterial culture and antimicrobial susceptibility test in the case of otitis externa, particularly for chronic or recurring cases. sterile swab samples were obtained from terminal part of vertical ear canals of 74 dogs with otitis externa for cytology, bacterial culture and antimicrobial susceptibility test. cytologic smears were stained using gram and giemsa staining methods. aerob ...201020526674
a 72-year-old man with intermittent fever, anemia and a history of coronary and peripheral artery disease. 201020526698
efficacy of a surgical scrub including 2% chlorhexidine acetate for canine superficial pyoderma.the clinical efficacy of a surgical scrub containing 2% chlorhexidine acetate (2ca; nolvasan® surgical scrub; fort dodge animal health, usa) was evaluated for the topical management of canine superficial pyoderma. the first study was a randomized, double-blind, controlled trial. the control was a shampoo containing 4% chlorhexidine gluconate (4cg; skin clinic shampoo; chd medics, goyang, korea). ten dogs with symmetrical lesions of canine superficial pyoderma were allocated to receive either 2ca ...201020529012
further diversity of the staphylococcus intermedius group and heterogeneity in the mboi restriction site used for staphylococcus pseudintermedius species identification.species identification of 200 beta-hemolysin-producing canine staphylococcal isolates was performed using a recently described polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) method (based on mboi restriction of a pta gene fragment), supplemented with biochemical testing and sequencing of housekeeping genes. the pcr-rflp method misclassified a small fraction (approximately 1%) of the staphylococcus pseudintermedius population as a result of heterogeneity in the mboi ...201020807936
reclassification of the listeria-camp test strain atcc 49444 staphylococcus aureus as staphylococcus pseudintermedius.atcc 49444, originally designated as staphylococcus aureus, has been cited as a component strain in the camp test for identification of listeria monocytogenes. a polyphasic study, in which molecular data were combined with cytochemical properties and physiological characteristics, identified this isolate as staphylococcus pseudintermedius. the nucleotide sequences of the 16s rrna and soda genes from atcc 49444 were determined and found to be identical to those of other s. pseudintermedius strain ...201020819366
staphylococcus intermedius is not only a zoonotic pathogen, but may also cause skin abscesses in humans after exposure to saliva.staphylococcus intermedius is a very rare human pathogen. there are only 16 cases in the literature that have described s. intermedius as a cause of infection in humans. most of these cases have been described in association with exposure to animals, mostly dogs. however, this pathogen can cause infection in healthy individuals even without exposure to animals.201020934636
prevalence of canine methicillin resistant staphylococcuspseudintermedius in a veterinary diagnostic laboratory in italy.the overall prevalence of methicillin-resistant staphylococcus pseudintermedius (mrsp) was 2% (10/590) among 590 canine specimens submitted to an italian veterinary diagnostic laboratory during a two-month period, and 21% (10/48) among staphylococcus intermedius group (sig) isolates. all methicillin-resistant strains exhibited additional resistance to fluoroquinolones, gentamicin, lincosamides, tetracyclines, and potentiated sulfonamides, belonged predominantly to spa type t02 and harboured sccm ...201020971485
multidrug- and methicillin resistant staphylococcus pseudintermedius as a cause of canine pyoderma: a case report.a case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant staphylococcus pseudintermedius (mrsp) is described. after initial diagnostics of mrsp, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. mrsp was constantly isolated from the skin and once from the nose. since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet's therapy. thereby, the mal ...201021038805
staphylococcus intermedius--rare pathogen of acute meningitis.we report the first case of acute meningitis caused by a rare, atypical pathogen. an 11-month-old infant was admitted to hospital with clinical symptoms typical of acute meningitis. cerebrospinal fluid analysis revealed an elevated neutrophil cell count and high proteins. microbiological examination of the fluid confirmed an atypical cause of meningitis--staphylococcus intermedius. antibiotic therapy with cefotaxime was successful and the child made a full recovery.201020053577
outbreak of neonatal diarrhea in farmed mink kits (mustella vison) associated with enterotoxigenic staphylococcus delphini.an outbreak of diarrhea on a large commercial mink farm affected 5,000 of 36,000 neonatal mink kits, with 2,000 dying within a 2-week period. affected kits were severely dehydrated, and their furcoats and paws were covered with yellow- to green-tinged mucoid feces. on necropsy, the small intestines of examined animals were markedly distended by serous to mucoid fluid. microscopically, there was prominent colonization of the intestinal villar epithelium by gram-positive bacterial cocci in the abs ...201020466860
the first case of a staphylococcus pseudintermedius infection after joint prosthesis implantation in a dog.we have reported a bacterial infection in a dog with progressive dysplasia of the hips. orthopedic surgery was performed. seven weeks prior to the surgery, the patient was bitten by another dog. the postimplantation wound exuded for four days after the surgery. microbiological analysis performed by standard identification techniques showed the presence of staphylococcus intermedius, but an additional molecular analysis indicated s. pseudintermedius. this was followed by an evaluation of antibiot ...201020734760
efficacy of tylosin tartrate on canine staphylococcus intermedius isolates in vitro.in the past 5 years, the incidence of canine skin infections caused by resistant strains of staphylococcus (pseud)intermedius has increased. many older antibiotics are used to treat these infections because the sensitivity can be demonstrated in vitro. additionally, many of these older drugs are efficacious and unlikely to induce multidrug resistance. more than a decade ago, the antibiotic tylosin tartrate was reported to be efficacious in vitro and in vivo against staphylococcus intermedius. th ...201020960412
widespread rapid emergence of a distinct methicillin- and multidrug-resistant staphylococcus pseudintermedius (mrsp) genetic lineage in europe.in order to gain a deeper insight into the phylogenetic background and diversity of methicillin-resistant s. pseudintermedius (mrsp) of animal origin, genetic relationships and clonal distribution among 146 european mrsp were examined using different molecular and phenotypical typing approaches. mrsp strains were derived from clinical microbiological specimens (mainly of small animal origin) sent in for diagnostic purposes from various veterinary facilities between 2005 and 2008. pulsed-field ge ...201020181441
high-fat feeding and staphylococcus intermedius infection impair beta cell function and insulin sensitivity in mongrel dogs.as obesity is a state of low-grade inflammation, we aimed to investigate the combined effect of high-fat diet and bacterial infection on beta-cell function and insulin sensitivity in dogs. we used 20 healthy, male, mongrel dogs randomly divided into four groups: control group-healthy, non-obese dogs; infected group-non-obese dogs with experimentally induced infection (staphylococcus intermedius); obese group-obese dogs (after 90 day high-fat diet) and obese-infected group-obese dogs with experim ...201020195753
antimicrobial activity of propolis extract against staphylococcus coagulase positive and malassezia pachydermatis of canine otitis.the aims of this study were to evaluate the antimicrobial potential of propolis extract by determining the minimum bactericidal concentration (mbc) for coagulase-positive staphylococcus isolates (staphylococcus aureus and staphylococcus intermedius) and the minimum fungicidal concentration (mfc) for malassezia pachydermatis isolates. the microorganisms were assayed using broth microdilution techniques. the mbc(90) was 21 mg ml(-1), and the mfc(90) was 5.3 mg ml(-1). the propolis extract was foun ...201019913365
differentiation of staphylococcus spp. by high-resolution melting analysis.high-resolution melting analysis (hrma) is a fast (post-pcr) high-throughput method to scan for sequence variations in a target gene. the aim of this study was to test the potential of hrma to distinguish particular bacterial species of the staphylococcus genus even when using a broad-range pcr within the 16s rrna gene where sequence differences are minimal. genomic dna samples isolated from 12 reference staphylococcal strains (staphylococcus aureus, staphylococcus capitis, staphylococcus caprae ...201021164574
Metastatic Complications From Staphylococcus intermedius, a Zoonotic Pathogen.Metastatic infection is an infrequent complication of non-aureus staphylococcal infection. Here we report a case of bloodstream infection due to Staphylococcus intermedius. To our knowledge, ours is the only known case of metastatic infection with S. intermedius.201122170938
identification of staphylococcus intermedius group by maldi-tof ms.the staphylococcus intermedius group includes s. intermedius, s. pseudintermedius and s. delphini, coagulase-positive bacteria commonly isolated from animals. the identification of organisms belonging to this group is presently carried out using molecular methods. this study assessed the suitability of maldi-tof ms for their identification. 69 strains of different biological and geographic origins, identified by partial hsp60 gene sequencing as s. intermedius (n=15), s. pseudintermedius (n=32) a ...201121300509
prevalence and antimicrogram of staphylococcus intermedius group isolates from veterinary staff, companion animals, and the environment in veterinary hospitals in korea.the staphylococcus intermedius bacterial group (sig) includes 3 distinct genetically heterogenous species: s. intermedius, s. pseudintermedius, and s. delphini. this pathogen group is associated with many opportunistic skin and ear infections in companion animals. human infections with s. intermedius and s. pseudintermedius isolates and the emergence of methicillin-resistant isolates have been recently reported, which emphasizes the importance of nationwide identification of sig isolate prevalen ...201121398446
genomic and surface proteomic analysis of the canine pathogen staphylococcus pseudintermedius reveals proteins which mediate adherence to the extracellular matrix.cell wall-associated (cwa) proteins made by gram-positive pathogens play a fundamental role in pathogenesis. staphylococcus pseudintermedius is a major animal pathogen responsible for the canine skin disease bacterial pyoderma. here we describe the bioinformatic analysis of the family of 18 predicted cwa proteins encoded in the genome of s. pseudintermedius strain ed99 and determine their distribution among a phylogenetically diverse panel of s. pseudintermedius clinical isolates, and closely-re ...201121576333
the occurrence and comparative phenotypic characteristics of staphylococcus spp. from healthy and diseased, household and shelter dogs, based on routine biochemical diagnostic methods.to determine the staphylococcal colonization pattern in healthy and diseased dogs, living in two particular environments, a number of microbiological samples were taken. overall, twenty dogs, either healthy or with infected skin lesions, were examined. in each case bacterial swabs were collected from the nasal mucosa, ear, perineum, lumbo-sacralis triangle, and from the infection sites if such were present. a total number of 104 isolates representing different staphylococcal species were isolate ...201121630570
meticillin-resistant strains of staphylococcus pseudintermedius in companion animals. 201121742696
antibiotic resistance of canine staphylococcus intermedius group (sig)--practical implications.a total of 221 sig strains were isolated from clinical samples of canine origin submitted to the diagnostic laboratory of the division of bacteriology and molecular biology at the warsaw university of life sciences in warsaw during the period 2006-2010. the aim of the study was to investigate the frequency of prevalence of methicillin-resistant sig strains and to determine the mic values of cephalotin, amoxicillin/clavulanic acid, ciprofloxacin, clindamycin, gentamicin, chloramphenicol, mupiroci ...201121721404
suspected seminal vesiculitis in an asian elephant (elephas maximus).a 32-year-old male asian elephant (elephas maximus) underwent routine transrectal stimulation for semen collection as part of an artificial insemination program. the procedure consisted of a preinsemination semen collection followed by two consecutive days of semen collections for artificial insemination. the second day's sample contained large numbers of inflammatory cells, intracellular bacteria, and phagocytized sperm. semen was submitted for culture and sensitivity. culture revealed acinetob ...201122950323
Antibiotic sensitivity of bacterial isolates from cases of canine dermatitis.Among 97 bacterial isolates, 74 strains of Staphylococcus spp developed from 95 swabs taken from skin lesions in dogs. Twenty-eight staphylococcal strains resistant to methicillin and/or oxacillin were identified and mecA expression was confirmed for 14 of these strains. S. aureus and S. intermedius group (SIG) strains were particularly relevant in our cases due to their antibiotic resistance leading to an increased veterinary and public health risk. We suggest a diagnostic protocol based on cyt ...201122143814
molecular characterization of staphylococcus pseudintermedius strains isolated from clinical samples of animal origin.the aim of this study was to determine the species distribution among 44 randomly selected clinical isolates (30 meca-positive and 14 meca-negative) of animal origin previously identified as staphylococcus intermedius by phenotypic tests and species-specific pcr amplification of the 16s rrna gene. for this purpose, we used a multiplex pcr for the detection of the nuc gene and restriction fragment length polymorphism analysis of pta gene amplified by pcr. both methods allow discrimination of stap ...201121874595
photodynamic therapy against common bacteria causing wound and skin infections.photodynamic therapy (pdt) uses the combination of a photosensitising (ps) agent and visible light. historically, various injectable ps agents have been used with medical grade lasers to treat neoplasia. the objective of this in vitro study was to determine whether pdt using topical aminolevulinic acid (ala) with a non-coherent light source would kill common wound infecting bacteria, namely, staphylococcus intermedius, streptococcus canis, pseudomonas aeruginosa, and escherichia coli. bacterial ...201122000592
determination of staphylococcal exotoxins, sccmec types, and genetic relatedness of staphylococcus intermedius group isolates from veterinary staff, companion animals, and hospital environments in korea.the staphylococcus (s.) intermedius group (sig) has been a main research subject in recent years. s. pseudintermedius causes pyoderma and otitis in companion animals as well as foodborne diseases. to prevent sig-associated infection and disease outbreaks, identification of both staphylococcal exotoxins and staphylococcal cassette chromosome mec (sccmec) types among sig isolates may be helpful. in this study, it was found that a single isolate (one out of 178 sig isolates examined) harbored the c ...201121897094
what's happened to staphylococcus intermedius? taxonomic revision and emergence of multi-drug resistance.staphylococcus intermedius has been the predominant coagulase-positive staphylococcus isolated from canine skin and mucosae and the most commonly reported staphylococcal pathogen in small animal practice for the last 35 years. although microbiological tests have historically indicated variability in biochemical characteristics amongst s. intermedius isolates from animals, an acceptable level of diagnostic accuracy for clinical purposes was readily achievable with routine phenotypic testing. howe ...201222251285
analysis of the bacterial diversity existing on animal hide and wool: development of a preliminary pcr-restriction fragment length polymorphism fingerprint database for identifying isolates.twenty-one bacterial strains were isolated from imported cattle hide and rabbit wool using two types of media, nutrient broth, and nutrient broth with serum. the bacteria identified were brevibacillus laterosporus, leclercia adecarboxylata, peptococcus niger, bacillus circulans, raoultella ornithinolytica, bacillus subtilis, bacillus cereus, bacillus thermobacillus, bacillus choshinensis, bacillus sphaericus, acinetobacter haemolyticus, sphingomonas paucimobilis, bacillus thuringiensis, staphylo ...201223451394
qualitative analysis of preputial and vaginal bacterial microbiota in owl monkeys (aotus azarai infulatus) raised in captivity.the aim of this study was to identify the aerobic bacteria of the preputial and vaginal microbiota in owl monkeys that have been raised in captivity and to evaluate the antimicrobial susceptibility of these bacteria by gender and social organization.201223278502
treating animal bites: susceptibility of staphylococci from oral mucosa of cats.infected wounds determined by cats' bites represent high costs to public health, and their adequate treatment relies on the knowledge of the antimicrobial susceptibility of bacterial agents found in the oral microbiota. members of the genus staphylococcus sp. belong to the microbiota of the oral mucosa of cats and are frequently involved in secondary infections of these wounds. this study aimed to evaluate the antimicrobial susceptibility of staphylococcus species isolated from oral mucosa of ca ...201223280142
arginine dehydrolase and β-gentiobiose cannot discriminate within the staphylococcus intermedius group. 201222921199
methicillin-resistant staphylococci: implications for our food supply?food-borne intoxication, caused by heat-stable enterotoxins produced by staphylococcus aureus, causes over 240,000 cases of food-borne illness in the united states annually. other staphylococci commonly associated with animals may also produce these enterotoxins. foods may be contaminated by infected food handlers during slaughter and processing of livestock or by cross-contamination during food preparation. s. aureus also causes a variety of mild to severe skin and soft tissue infections in hum ...201223253164
comparative genomics of the staphylococcus intermedius group of animal pathogens.the staphylococcus intermedius group consists of three closely related coagulase-positive bacterial species including s. intermedius, staphylococcus pseudintermedius, and staphylococcus delphini. s. pseudintermedius is a major skin pathogen of dogs, which occasionally causes severe zoonotic infections of humans. s. delphini has been isolated from an array of different animals including horses, mink, and pigeons, whereas s. intermedius has been isolated only from pigeons to date. here we provide ...201222919635
antimicrobial resistance of staphylococcus pseudintermedius.staphylococcus pseudintermedius, staphylococcus intermedius and staphylococcus delphini together comprise the s. intermedius group (sig). within the sig, s. pseudintermedius represents the major pathogenic species and is involved in a wide variety of infections, mainly in dogs, but to a lesser degree also in other animal species and humans. antimicrobial agents are commonly applied to control s. pseudintermedius infections; however, during recent years s. pseudintermedius isolates have been iden ...201222681522
mustelidae are natural hosts of staphylococcus delphini group a.according to the current taxonomy, the staphylococcus intermedius group (sig) comprises of at least three distinct species. while s. intermedius and s. pseudintermedius are associated with specific hosts (pigeons and dogs, respectively), the natural host of s. delphini remains unclear. we analysed 158 sig isolates from less studied animal species belonging to the order carnivora, including mink (n=118), fox (n=33), badger (n=6) and ferret (n=1). species identification was performed by nuc pcr in ...201222542523
staphylococcus intermedius infections: case report and literature review.staphylococcus intermedius is part of the normal skin and oral flora of dogs. case reports of human infections are rare, but the true incidence is unknown because the pathogen is frequently misidentified as staphylococcus aureus. reported cases range from soft tissue infections to brain abscess. most reported cases in humans have been related to dog exposure. we report a case of a 73 year old female with s. intermedius surgical wound infection one month following a left elbow total arthroplasty. ...201324470954
a new class of quorum quenching molecules from staphylococcus species affects communication and growth of gram-negative bacteria.the knowledge that many pathogens rely on cell-to-cell communication mechanisms known as quorum sensing, opens a new disease control strategy: quorum quenching. here we report on one of the rare examples where gram-positive bacteria, the 'staphylococcus intermedius group' of zoonotic pathogens, excrete two compounds in millimolar concentrations that suppress the quorum sensing signaling and inhibit the growth of a broad spectrum of gram-negative beta- and gamma-proteobacteria. these compounds we ...201324098134
phenotypic identification of staphylococcus intermedius may be inaccurate. 201323355726
novel methicillin-resistant coagulase-negative staphylococcus clone isolated from patients with haematological diseases at the blood bank centre of amazon, brazil.methicillin-resistant staphylococcus remains a severe public health problem worldwide. this research was intended to identify the presence of methicillin-resistant coagulase-negative staphylococci clones and their staphylococcal cassette chromosome mec (sccmec)-type isolate from patients with haematologic diseases presenting bacterial infections who were treated at the blood bank of the state of amazonas in brazil. phenotypic and genotypic tests, such as sccmec types and multilocus sequence typi ...201323579805
investigations on the efficacy of routinely used phenotypic methods compared to genotypic approaches for the identification of staphylococcal species isolated from companion animals in irish veterinary hospitals.identification of staphylococci to species level in veterinary microbiology is important to inform therapeutic intervention and management. we report on the efficacy of three routinely used commercial phenotypic methods for staphylococcal species identification, namely api staph 32 (biomérieux), rapid (remel) and staph-zym (rosco diagnostica) compared to genotyping as a reference method to identify 52 staphylococcal clinical isolates (23 coagulase positive; 29 coagulase negative) from companion ...201323635328
camp test detected staphylococcus delphini atcc 49172 beta-haemolysin production.through a camp test, we first observed a staphylococcus delphini strain (atcc 49172) to release beta-haemolysin. production of the latter in this coagulase-positive species of the 'staphylococcus intermedius group', in fact, has been labeled to be undetermined, thus far. of course, a wider number of strains have to be investigated in order to define whether this property is constitutive (like in staphylococcus (pseud)intermedius), or strain-dependent (like in staphylococcus aureus), and which cl ...201324730145
antimicrobial susceptibility profiles of staphylococcus intermedius isolates from clinical cases of canine pyoderma in south africa.successful treatment of canine pyoderma has become compromised owing to the development of antimicrobial resistance with accompanying recurrence of infection. canine skin samples submitted to a veterinary diagnostic laboratory for microbiological culture and sensitivity between january 2007 and june 2010, from which staphylococcus intermedius was isolated, were selected for this investigation. antimicrobial resistance of s. intermedius was most prevalent with reference to ampicillin followed by ...201323718758
reply to "phenotypic identification of staphylococcus intermedius may be inaccurate". 201323355727
a case of antibacterial-responsive mucocutaneous disease in a seven-year-old dwarf lop rabbit (oryctolagus cuniculus) resembling mucocutaneous pyoderma of dogs.a seven-year-old, ovariohysterectomised female dwarf lop rabbit (oryctolagus cuniculus) was referred with severe swelling and erythema of the mucocutaneous junctions of the lips, nares and vulva. bilateral, severe periocular dermatitis was also present. heavy pure growths of a member of the staphylococcus intermedius group were cultured from nasal and aural swabs and skin biopsies. other possible differential diagnoses were eliminated by standard tests. the clinical features and histopathologica ...201323346925
bacteria species identification by the statistical analysis of bacterial colonies fresnel patterns.it was demonstrated that statistical analysis of bacteria colonies fresnel patterns recorded in the optical system with converging spherical wave illumination is suitable for highly effective bacteria species classification. the proposed method includes fresnel patterns recording followed by image processing and the statistical analysis based on feature extraction, feature selection, classification and classification performance methods. examination performed on various bacteria species (salmone ...201323669989
nasal adenocarcinoma and secondary chronic sinusitis in a hyacinth macaw (anodorhynchus hyacinthinus).an adult male hyacinth macaw (anodorhynchus hyacinthinus) that presented for acute onset nasal discharge and dyspnea had purulent discharge from the right naris and serosanguineous discharge from the left naris on physical examination. results of a complete blood count revealed severe leukocytosis with a mature heterophilia. computed tomography scans showed a large amount of soft-tissue attenuating material within the infraorbital sinus and associated diverticula. aerobic culture results of the ...201425115043
synergistic hemolysis between β-lysin-producing staphylococcus species and rothia nasimurium in primary cultures of clinical specimens obtained from dogs.nonhemolytic isolates of catalase-positive, gram-positive cocci were observed that produced strong synergistic hemolysis with colonies of staphylococcus pseudintermedius and staphylococcus schleiferi subsp. coagulans in primary cultures of clinical specimens from dogs. the isolates appeared similar to nonhemolytic staphylococci but were not identified with conventional biochemical tests or commercial biochemical test kits. partial 16s ribosomal rna gene sequences from each of 9 tested isolates w ...201424760132
[new insight into bacterial zoonotic pathogens posing health hazards to humans].this article presents the problem of evolutionary changes of zoonotic pathogens responsible for human diseases. everyone is exposed to the risk of zoonotic infection, particularly employees having direct contact with animals, i.e. veterinarians, breeders, butchers and workers of animal products' processing industry. the article focuses on pathogens monitored by the european centre for disease prevention and control (ecdc), which has been collecting statistical data on zoonoses from all european ...201425902698
cervical necrotizing fasciitis resulting in acupuncture and herbal injection for submental lipoplasty.acupuncture is used for some conditions as an alternative to medication or surgical intervention. several complications had been reported, and they are generally due to physical injury by the needle or transmission of diseases. necrotizing fasciitis is an uncommon infection, but potentially lethal, especially when associated with systemic disorders such as diabetes. the authors report a case of serious cervical necrotizing fasciitis that developed after acupuncture and herbal injection treatment ...201425148627
design and application of a loop-mediated isothermal amplification assay for the rapid detection of staphylococcus pseudintermedius.staphylococcus pseudintermedius is a commensal and opportunistic pathogen of dogs. it is mainly implicated in canine pyoderma, as well as other suppurative conditions of dogs. although bacterial culture is routinely used for clinical diagnosis, molecular methods are required to accurately identify and differentiate s. pseudintermedius from other members of the staphylococcus intermedius group. these methods, owing largely to their cost, are not easy to implement in nonspecialized laboratories or ...201424398904
species differentiation within the staphylococcus intermedius group using a refined maldi-tof ms database.among coagulase-positive staphylococci of animal origin, the members of the staphylococcus intermedius-group (sig: s. intermedius, staphylococcus pseudintermedius and staphylococcus delphini) are important opportunistic pathogens in different animal hosts and occasionally in humans. however, the unambiguous species diagnosis of sig is often challenging. therefore, matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) -based sig-identification with bruker mic ...201424807701
biofilm-infected wounds in a dog.a 4-year-old spayed female mastiff was evaluated for treatment of chronic nonhealing pressure wounds over both elbow regions resulting from attempts at hypertrophic callus excision.201424568112
comparison of cefoxitin disk diffusion test and meca gene pcr results for methicillin resistance detection in staphylococcus intermedius group isolates from canine origin in brazil.the study evaluated cefoxitin disk diffusion tests breakpoints and their correlation to meca gene pcr results for detecting methicillin-resistant staphylococcus intermedius group (mrsp) isolates from dogs in brazil. agreement using proposed breakpoint (resistant ≤ 30 mm) was encouraging. the current study reinforces that an epidemiological breakpoint can be established to predict presence of mrsp.201424948938
antibiotic resistance and molecular characterization of ophthalmic staphylococcus pseudintermedius isolates from dogs.the prevalence, virulence potential, and antibiotic resistance of ophthalmic staphylococcus pseudintermedius (sp) isolated from dogs were examined. sixty-seven staphylococcus species were isolated from ophthalmic samples and surveyed for species-specific sequences in the staphylococcus intermedius group (sig) nuclease gene (sinuc), exfoliative toxin gene for sig (siet), and antibiotic resistance genes (blaz and meca). pcr-restriction fragment length polymorphism analysis of the pta gene was also ...201424690601
are staphylococcus intermedius infections in humans cases of mistaken identity? a case series and literature review.staphylococcus intermedius and staphylococcus pseudintermedius are difficult to distinguish using conventional microbiological methods. molecular diagnostic tools change our understanding of the epidemiology of these 2 organisms. in this study, we present (1) a detailed review of the current literature on molecular diagnostics and (2) a case series in which misidentification was proven in 1 case. we conclude that s pseudintermedius is a more common human pathogen than previously recognized.201526509181
increasing antimicrobial resistance in clinical isolates of staphylococcus intermedius group bacteria and emergence of mrsp in the uk.frequencies of antimicrobial resistance were determined amongst 14,555 clinical staphylococcus intermedius group (sig) isolates from uk dogs and cats to estimate resistance trends and quantify the occurrence of meticillin-resistant staphylococcus pseudintermedius (mrsp). reports from two diagnostic laboratories (13,313 general submissions, 1242 referral centre only submissions) were analysed retrospectively (2003/2006-2012). mrsp were defined by phenotypic resistance to meticillin and concurrent ...201525376505
antioxidant activity and antibacterial effects on clinical isolated streptococcus suis and staphylococcus intermedius of extracts from several parts of cladogynos orientalis and their phytochemical screenings.the in vitro antioxidant and antibacterial assays against clinically isolated streptococcus suis and staphylococcus intermedius of the extracts prepared by decoction and ethanolic reflux of different parts of chettaphangki (cladogynos orientalis zipp. ex span), including the leaves, roots, and stems, using 1,1-diphenyl-2-picrylhydrazyl (dpph) scavenging assay and disc diffusion method were conducted. quantitative analysis of total phenolic and total flavonoid contents in the extracts using spect ...201526347795
an evaluation of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the identification of staphylococcus pseudintermedius isolates from canine infections.it has been proposed, based on taxonomic and molecular studies, that all canine isolates belonging to staphylococcus intermedius group (sig) should be renamed staphylococcus pseudintermedius. however, isolates of sig and other coagulase-positive staphylococci share many phenotypic characteristics, which could lead to misidentification. the accuracy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) for identifying s. pseudintermedius isolates obtained ...201525680922
improved detection of staphylococcus intermedius group in a routine diagnostic laboratory.the staphylococcus intermedius group (sig) includes zoonotic pathogens traditionally associated with dog bites. we describe a simple scheme for improved detection of sig using routine laboratory methods, report its effect on isolation rates, and use sequencing to confirm that, apart from one atypical sig strain, most isolates are staphylococcus pseudintermedius.201525502532
characterisation of nasal staphylococcus delphini and staphylococcus pseudintermedius isolates from healthy donkeys in tunisia.staphylococcus intermedius group (sig) bacteria can colonise the nares of some animals but are also emerging pathogens in humans and animals.201524913693
review of bacterial and viral zoonotic infections transmitted by dogs.dogs are a major reservoir for zoonotic infections. dogs transmit several viral and bacterial diseases to humans. zoonotic diseases can be transmitted to human by infected saliva, aerosols, contaminated urine or feces and direct contact with the dog. viral infections such as rabies and norovirus and bacterial infections including pasteurella, salmonella, brucella, yersinia enterocolitica, campylobacter, capnocytophaga, bordetella bronchiseptica, coxiella burnetii, leptospira, staphylococcus inte ...201528316698
antimicrobial susceptibility monitoring of bacterial pathogens isolated from respiratory tract infections in dogs and cats across europe: compath results.compath is a pan-european resistance monitoring programme collecting bacterial pathogens from dogs and cats. we present data for respiratory tract infection (rti) isolates collected between 2008 and 2010. antimicrobial minimal inhibitory concentrations (mics) were determined and susceptibility calculated following clinical laboratory standards institute (clsi) standards for veterinary medicine. the main pathogen from dogs was staphylococcus intermedius group (49/215, 22.8%) which was >90% suscep ...201627374906
linoleic acid salt with ultrapure soft water as an antibacterial combination against dermato-pathogenic staphylococcus spp.skin colonization of staphylococcus spp. critically affects the severity of dermatitis in humans and animals. we examined different types of fatty acid salts for their antibacterial activity against staphylococcus spp. when used in ultrapure soft water (upsw). we also evaluated their therapeutic effect on a spontaneous canine model of dermatitis.201626606689
what's in a name? the impact of accurate staphylococcus pseudintermedius identification on appropriate antimicrobial susceptibility testing.bacteria in the staphylococcus intermedius group, including staphylococcus pseudintermedius, often encode meca-mediated methicillin resistance. reliable detection of this phenotype for proper treatment and infection control decisions requires that these coagulase-positive staphylococci are accurately identified and specifically that they are not misidentified as s. aureus. as correct species level bacterial identification becomes more commonplace in clinical laboratories, one can expect to see c ...201626763965
evaluation of an immunochromatographic assay for rapid detection of penicillin-binding protein 2a in human and animal staphylococcus intermedius group, staphylococcus lugdunensis, and staphylococcus schleiferi clinical isolates.the performance of a rapid penicillin-binding protein 2a (pbp2a) detection assay, the alere pbp2a culture colony test, was evaluated for identification of pbp2a-mediated beta-lactam resistance in human and animal clinical isolates of staphylococcus intermedius group, staphylococcus lugdunensis, and staphylococcus schleiferi. the assay was sensitive and specific, with all pbp2a-negative and pbp2a-positive strains testing negative and positive, respectively.201626677248
evaluation of oxacillin and cefoxitin disk and mic breakpoints for prediction of methicillin resistance in human and veterinary isolates of staphylococcus intermedius group.staphylococcus pseudintermedius is a coagulase-positive species that colonizes the nares and anal mucosa of healthy dogs and cats. human infections with s. pseudintermedius range in severity from bite wounds and rhinosinusitis to endocarditis; historically, these infections were thought to be uncommon, but new laboratory methods suggest that their true incidence is underreported. oxacillin and cefoxitin disk and mic tests were evaluated for the detection of meca- or mecc-mediated methicillin res ...201626607988
screening for antibacterial and antioxidant activities and phytochemical analysis of oroxylum indicum fruit extracts.oroxylum indicum, which is called pheka in thai, is a traditional thai plant in the bignoniaceae family with various ethnomedical uses such as as an astringent, an anti-inflammatory agent, an anti-bronchitic agent, an anti-helminthic agent and an anti-microbial agent. the young fruits of this plant have also been consumed as vegetables. however, there has been no report concerning its antibacterial activities, especially activities related to clinically isolated pathogenic bacteria and the in vi ...201627070560
in vitro antimicrobial susceptibility of staphylococcus pseudintermedius isolates of human and animal origin.mic results for 115 staphylococcus intermedius group isolates are presented. of these, 33% were methicillin resistant, among which 51.4% were susceptible to doxycycline, 29.7% to clindamycin, and 21.6% to trimethoprim-sulfamethoxazole. all of the isolates were susceptible to ceftaroline, daptomycin, linezolid, nitrofurantoin, quinupristin-dalfopristin, rifampin, tigecycline, and vancomycin. of all the isolates, 82.6%, 67.8%, and 23.5% were susceptible to ciprofloxacin, erythromycin, and penicill ...201626962087
tetracycline resistance phenotypes and genotypes of coagulase-negative staphylococcal isolates from bubaline mastitis in egypt.this study was devoted to elucidate the tetracycline resistance of coagulase-negative staphylococci (cns) derived from normal and subclinical mastitic (scm) buffaloes' milk in egypt.201728717325
skin infection caused by a novel strain of staphylococcus pseudintermedius in a siberian husky dog owner.introduction.staphylococcus pseudintermedius, an opportunistic pathogen of dogs and cats, is rarely reported to cause infection in humans. here, we describe a case of severe skin infection caused by s. pseudintermedius, in a 47-year-old male, a dog owner; to the best of our knowledge, this is the first such case reported from scotland. case presentation. the patient presented with a short history of a severe ecthyma-like lesion on his forehead, with smaller lesions on his abdomen and legs. bacte ...201728663821
the prevalence of antibiotic-resistant bacteria (arb) in waters of the lower ballona creek watershed, los angeles county, california.screening for the prevalence of antibiotic-resistant bacteria (arb) was done at the ballona creek and wetlands, an urban-impacted wetland system in los angeles, california. the goals were (1) to assess the overall prevalence of arb, and (2) compare differences in arb abundance and the types of antibiotic resistance (ar) among the following sample types: lagoon water from del rey lagoon, urban runoff from ballona creek, and water from the ballona wetlands (tidal water flooding in from the adjacen ...201728488226
pyogenic ventriculitis and ventricular empyema associated with staphylococcus pseudintermedius in a puppy.a 40-day-old male, blue heeler puppy with hindlimb ataxia, nystagmus, apathy, motor incoordination and hyperaesthesia of the forelimbs died 3 days after the onset of clinical signs. significant gross findings included cerebellar herniation, cerebral oedema and dilation of the third and right lateral cerebral ventricles due to the accumulation of a purulent exudate. histopathological examination revealed pyogenic ventriculitis and purulent meningoencephalitis. pure colonies of a coagulase-positiv ...201728041618
antimicrobial susceptibility monitoring of bacterial pathogens isolated from urinary tract infections in dogs and cats across europe: compath results.compath is a pan-european antimicrobial surveillance program collecting bacterial pathogens from dogs and cats not recently exposed to antimicrobials. we present minimum inhibitory concentration data obtained using clinical and laboratory standards institute methodology for 616 urinary tract infection (uti) isolates collected between 2008 and 2010. in both dogs and cats, the most common pathogen was escherichia coli (59.8% and 46.7%, respectively). antimicrobial activity against e. coli in dogs ...201728384093
clinical pathology of the critically endangered gough bunting (rowettia goughensis).the gough bunting (rowettia goughensis) is indigenous to gough island and critically endangered due to predation by invasive house mice (mus musculus). a planned ecosystem restoration by eradication of house mice via aerially delivered rodenticide requires a reproductively balanced population of gough buntings being held in captivity to avoid primary and secondary poisoning. to aid disease detection during the period of captivity, gough buntings (n = 25; five adult females, 15 adult males and fi ...201728185624
evaluation of pyrrolidonyl arylamidase activity in staphylococcus delphini.clinical reference textbooks lack data for pyrrolidonyl arylamidase (pyr) activity in staphylococcus delphini this study evaluated pyr activities of 21 s. delphini strains by reference broth, rapid disc, and rapid slide methods. species and subgroup identifications were confirmed by nucleic acid-based methods and included nine group a and 12 group b strains. testing by rapid pyr methods with products from four manufacturers was performed at two testing locations, and, with the exception of one s ...201728003425
presence of environmental coagulase-positive staphylococci, their clonal relationship, resistance factors and ability to form biofilm.coagulase-positive staphylococci (cops) are opportunistic pathogens carrying various mechanisms of resistance that have a large number of virulence factors, and whose ability to induce illness is associated with the host. this study aimed to investigate the presence of environmental coagulase-positive staphylococci, their susceptibility profile, clonal relationship and ability to form biofilm. the 16s rrna genes from cops isolates were analyzed, and their antibiotic susceptibility was evaluated ...201728017482
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