Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| emerging chloramphenicol resistance in staphylococcus lentus from mink following chloramphenicol treatment: characterisation of the resistance genes. | a total of 26 staphylococcal strains isolated from mink with urinary tract infections as well as from the environment of the mink were examined for antibiotic resistance and prevalence of plasmids mediating resistance to the antibiotics applied for prophylactic or therapeutic purposes. chloramphenicol resistance (cmr) which occurred in fourteen of the eighteen staphylococcus lentus strains, but in none of the staphylococcus intermedius and staphylococcus xylosus strains, was shown to be mediated ... | 1994 | 7801525 |
| antimicrobial effect of combinations of edta-tris and amikacin or neomycin on the microorganisms associated with otitis externa in dogs. | combinations of edta-tris and two aminoglycoside antibiotics (amikacin and neomycin) were tested for synergistic activities against the microorganisms associated with otitis externa in dogs and for the solutions' stability over time. synergistic activity was observed when edta-tris plus amikacin and edta-tris plus neomycin were tested against staphylococcus intermedius, proteus mirabilis, pseudomonas aeruginosa, and escherichia coli, but not against candida albicans. stability studies over a 3-m ... | 1994 | 7831753 |
| pathogenic staphylococci and staphylococcal infections in canaries. | staphylococcus aureus was isolated from six cases of bacterial infection in canaries, staphylococcus hyicus from three and staphylococcus intermedius from one case. staphylococcus aureus was most often involved in outbreaks of septicaemia, with or without 'megabacterium' proventriculitis. the other coccal species were mainly complicating disease caused by atoxoplasma (lankesterella), chlamydia psittaci and canary pox. | 1994 | 18671080 |
| clinical pharmacologic aspects of cefixime in dogs. | the minimal inhibitory concentration (mic) of cefixime, a new third-generation orally administered caphalosporin, was determined for reference and clinical isolates from dogs. the mic of the drug for all but 1 of the 18 enterobacteriaceae isolates tested, 1 pasteurella canis, 1 rhodococcus equi, 1 streptococcus canis, and 1 streptococcus group g isolate, was less than 1.0 microgram/ml. the mic for 9 staphylococcus intermedius isolates ranged from 1.56 to 6.25 micrograms/ml and, for 8 sta aureus ... | 1995 | 7661460 |
| [susceptibility of bacterial isolates from the equine respiratory tract to trimethoprim, sulfadoxine, sulfadimethoxine and combinations of these compounds]. | using a broth microdilution technique, the in vitro susceptibility of bacterial isolates from the equine respiratory tract to trimethoprim, sulfadoxine, sulfadimethoxine, and combinations of these compounds was determined. the bacterial strains (n = 88) isolated recently from horses with respiratory symptoms belonged to the following species: streptococcus equi subsp. zooepidemicus (n = 34), streptococcus equi subsp. equi (n = 22), staphylococcus aureus (n = 9), klebsiella pneumoniae (n = 7), rh ... | 1995 | 7624856 |
| characterisation of a synergohymenotropic toxin produced by staphylococcus intermedius. | staphylococcal synergohymenotropic (sht) toxins damage membranes of host defence cells and erythrocytes by the synergy of two secreted and non-associated proteins: class s and class f components. whereas panton-valentine leucocidin (pvl), gamma-hemolysin and luk-m from staphylococcus aureus are members of this toxin family, a new bi-component toxin (luks-i + lukf-i) from staphylococcus intermedius, a pathogen for small animals, was characterised and sequenced. it is encoded as a luk-i operon by ... | 1995 | 7498527 |
| in vitro indices of tissue adherence in staphylococcus intermedius. | in vitro indices of adherence showed that strains of staphylococcus intermedius from lesions of canine pyoderma differed from strains isolated from normal carrier sites in that a significantly greater proportion of pyoderma strains adhered to extracellular matrix proteins whilst fewer adhered to polystyrene. slime production and a hydrophobicity index did not differ between the groups. this suggests that exposure of extracellular matrix proteins due to underlying disease may result in the select ... | 1995 | 7766075 |
| comparative in vitro activity of 11 beta-lactam antibiotics against 91 staphylococcus intermedius strains isolated from staphylococcal dermatitis in dogs. | a study was made of the susceptibility of 91 strains of staphylococcus intermedius, isolated from staphylococcal dermatitis of dogs, to 11 beta-lactam antimicrobial agents. the activity of these antimicrobial agents against all strains was tested at ph 7.2 and 8.5. finally, a comparative study was made of the susceptibility of these micro-organisms, dividing them into six groups: beta-lactamase positive and negative, methicillin resistant and susceptible and oxacillin resistant and susceptible. ... | 1995 | 8592904 |
| catheter-related bacteremia associated with coagulase-positive staphylococcus intermedius. | we report a case of catheter-related bacteremia in a 63-year-old patient caused by staphylococcus intermedius. clinical resolution of the infection was obtained after removal of the intravenous device and antibiotic treatment. this observation emphasizes the risk of confusion between s. intermedius and staphylococcus aureus if only a coagulase test is done. | 1995 | 7494062 |
| antibacterial activity of marbofloxacin. a new fluoroquinolone for veterinary use against canine and feline isolates. | marbofloxacin is a new fluoroquinolone developed exclusively for veterinary use. minimum inhibitory concentrations of marbofloxacin were assessed for 816 recent isolates associated with canine or feline diseases. marbofloxacin showed a broad spectrum of activity against gram-negative and gram-positive bacteria. in vitro rates of killing of marbofloxacin and enrofloxacin were compared against strains of staphylococcus intermedius and pasteurella multocida, and the results showed no marked differe ... | 1995 | 8583541 |
| antimicrobial susceptibility and rrna gene restriction patterns among staphylococcus intermedius from healthy dogs and from dogs suffering from pyoderma or otitis externa. | a total of 60 staphylococcus intermedius strains from dogs were investigated by their sensitivity to various antibiotics (50 strains) and by their rrna gene restriction patterns (ribotyping) (60 strains). fifteen isolates were from healthy dogs, 9 with otitis externa, and 36 with pyoderma, including 10 strains from a previous study. sixty per cent of the 50 strains tested for antibiotic susceptibility demonstrated resistance to penicillin, 24% to spiramycin, 20% to tetracycline, 16% to chloramph ... | 1995 | 7502950 |
| comparative ribotyping of staphylococcus intermedius from dogs, pigeons, horses and mink. | strains of staphylococcus (s.) intermedius from dogs, pigeons, horses and mink were typed by comparison of rrna gene restriction fragment length polymorphisms (ribotyping) and the resulting ribotypes examined by cluster-analysis. digestion of whole-cell dna with hindiii resulted in 9 ribotypes with 3 to 4 bands. separation of isolates from different host animal species was not possible. ecori yielded 11 different patterns with 4 to 9 fragments. the ecori-ribotypes of all canine strains grouped i ... | 1995 | 7544518 |
| multicenter evaluation of arbitrarily primed pcr for typing of staphylococcus aureus strains. | fifty-nine isolates of staphylococcus aureus and a single strain of staphylococcus intermedius were typed by arbitrarily primed pcr (ap-pcr). to study reproducibility and discriminatory abilities, ap-pcr was carried out in seven laboratories with a standardized amplification protocol, template dna isolated in a single institution, and a common set of three primers with different resolving powers. the 60 strains could be divided into 16 to 30 different genetic types, depending on the laboratory. ... | 1995 | 7650182 |
| chloramphenicol resistance in staphylococcus intermedius from a single veterinary centre: evidence for plasmid and chromosomal location of the resistance genes. | a total of seven staphylococcus intermedius cultures isolated from cases of canine pyoderma were investigated for the genetic basis of chloramphenicol resistance (cmr). all of these s. intermedius isolates mediated cmr via the expression of the cm-inactivating enzyme chloramphenicol acetyltransferase (cat); the respective cat genes were found to be located on small multicopy plasmids of 3.1 to 4.1 kb in four of the seven cultures. the four cmr plasmids, designated pscs20-23, differed upon restri ... | 1995 | 7740754 |
| insertion elements in staphylococcus intermedius. | staphylococcus intermedius cultures from dogs, pigeons, horses and mink were investigated for the prevalence of the insertion elements is256 and is257 in relation to their antibiotic resistance. copies of is256 could not be detected in any of the staph. intermedius isolates tested whereas single copies of is257 occurred in the isolates from dogs and horses. the mink strains did not harbour is257 elements, whereas staph. intermedius isolates from pigeons carried multiple copies of is257 as predic ... | 1995 | 7766077 |
| diversity of adenine conversion in staphylococci. | the special position of staphylococcus intermedius in comparison with staphylococcus saprophyticus, staphylococcus chromogenes, and staphylococcus hyicus was shown by deamination of adenine to hypoxanthine. s. chromogenes and s. hyicus converted adenine to adenosine. estimation of adenine conversion may be used for differentiation of s. intermedius from other coagulase-positive staphylococci. | 1996 | 8893524 |
| genomic dna fingerprinting, using pulsed-field gel electrophoresis, of staphylococcus intermedius isolated from dogs. | to investigate the degree of polymorphism in the pulsed-field gel electrophoresis (pfge) patterns of staphylococcus intermedius and to assess the value of this typing method for discriminating strains. | 1996 | 8896684 |
| prevalence of coagulase-positive staphylococci, other than staphylococcus aureus, in bovine mastitis. | to determine prevalence and relevance of coagulase-positive staphylococcus hyicus and s intermedius intramammary infections (imi) in dairy cows and determine the ability of the 4-hour tube coagulase (tc) test to differentiate the coagulase-positive staphylococci (cps). | 1996 | 8720238 |
| genomic variability of staphylococcus aureus and the other coagulase-positive staphylococcus species estimated by macrorestriction analysis using pulsed-field gel electrophoresis. | the genomic dnas of 95 culture collection and hospital staphylococcus aureus subsp. aureus strains of various origins, as well as the genomic dnas of other coagulase-positive staphylococcus species, were cleaved with restriction endonuclease smai and subjected to pulsed-field gel electrophoresis. the levels of similarity of the smai restriction patterns of the s. aureus subsp. aureus strains varied from 30 to 100%, which is considered characteristic of this species; thus, these organisms belonge ... | 1996 | 8573498 |
| on the nature and use of randomly amplified dna from staphylococcus aureus. | various dna-based methods have been introduced to genetically type staphylococcus aureus strains but not a single technique is universally applicable. in order to search for dna probes suitable for differentiating strains, randomly amplified polymorphic dna patterns were generated for 243 s. aureus strains and a single isolate of staphylococcus intermedius. all fingerprints were examined for unique amplicons, and the nature of 42 of these dna fragments was investigated. partial dna sequences wer ... | 1996 | 8897181 |
| further studies on the efficacy of tylosin tablets for the treatment of pyoderma due to staphylococcus intermedius infection in dogs. | 1996 | 8896878 | |
| in vitro activity of fifteen antimicrobial agents against methicillin-resistant and methicillin-susceptible staphylococcus intermedius. | in this study the susceptibility of 91 methicillin-resistant and -susceptible staphylococcus intermedius strains (mrsi and mssi, respectively) against 15 antimicrobial agents was determined. the activity of the antimicrobial agents was studied at ph 7.2 and ph 8.5. methicillin was more active at ph 7.2 (28 strains methicillin-resistant) than at ph 8.5 (55 strains methicillin-resistant). gentamicin showed excellent activity, with only 3 strains resistant at ph 8.5. however, gentamicin would have ... | 1996 | 8735419 |
| tylosin in the treatment of canine superficial pyoderma. | thirty dogs with superficial pyoderma were treated orally with tylosin at a dose of 20 mg/kg twice daily for three weeks. staphylococcus intermedius was recovered from 21 (70 per cent) of the dogs. twenty-two of the dogs were free of clinical signs after three weeks of treatment and two dogs responded to a further two weeks of treatment, giving a total response rate of 80 per cent. five cases (16.6 per cent) failed to respond and three of these subsequently responded to other antibacterial treat ... | 1996 | 8873399 |
| comparison of the beta-toxins from staphylococcus aureus and staphylococcus intermedius. | the beta-toxins produced by staphylococcus aureus and staphylococcus intermedius were purified to homogeneity from culture supernatants. although the toxin from s. aureus has been throughly studied, less is known about its unique counterpart from s. intermedius. this is the first reported purification and analysis of the s. intermedius beta-toxin. both toxins have similar enzymatic properties, belong to the class of neutral sphingomyelinases c, and have a high specificity for sphingomyelin. they ... | 1996 | 8914839 |
| clinical and microbiological observations of early polytetrafluoroethylene membrane exposure in guided bone regeneration. case reports in primates. | premature membrane exposure at one week is described in 3 macaca mulatta monkeys as part of a sequence of experiments on guided bone regeneration. clinical sequelae include redness, edema, and tissue slough. bacteroides fragilis, streptococcus pneumoniae, prevotella intermedia, and staphylococcus intermedius were detected at all prematurely exposed sites. pseudomonas maltophilia, strept, pneumoniae, and p. intermedia were the predominant organisms detected and consisted of more than 10% of the t ... | 1996 | 8708956 |
| a high molecular weight protein from staphylococcus intermedius cross-reacts with staphylococcus aureus enterotoxin antibodies. | enterotoxin production by staphylococcus species other than staphylococcus aureus has been reported. staphylococcus strains (104 in toto) representing twelve species and subspecies were examined for enterotoxins using a commercial staphylococcal enterotoxin elisa immunoassay (tecra, international bioproducts). staphylococcus intermedius (24 strains) and s. aureus (7 strains) were positive with this test. western blots of s. aureus exoproteins demonstrated proteins of approximately 30 kd, consist ... | 1996 | 9178533 |
| characterization of the canine type c enterotoxin produced by staphylococcus intermedius pyoderma isolates. | the type c staphylococcal enterotoxins (secs) are a group of highly conserved proteins with substantial antigenic cross-reactivity. although staphylococcus intermedius and coagulase-positive species of staphylococci are reported to produce sec and other ses, toxins produced by species other than staphylococcus aureus have not been previously characterized. in this study we report the molecular, biological, and immunological properties of the canine sec (seccanine) expressed by pathogenic isolate ... | 1997 | 9169773 |
| frequency of isolation of staphylococcus intermedius from humans. | we collected 3,397 consecutive isolates of coagulase-positive staphylococci from various specimens of hospitalized patients. all were retrospectively classified as staphylococcus aureus, except two which were identified as s. intermedius: one isolated from the nasal flora of a healthy carrier and the other isolated from pleural fluid, probably as a sample contaminant. | 1997 | 9230402 |
| aspects of the humoral immune response to staphylococcus intermedius in dogs with superficial pyoderma, deep pyoderma and anal furunculosis. | bacterial infection (pyoderma) of the canine skin is largely caused by staphylococcus intermedius and may be a superficial or deep infection. pyoderma may be a primary, idiopathic disease or secondary to a range of other dermatological disorders. in this study, the serum concentrations of igg, iga, antistaphylococcal igg and antistaphylococcal iga were measured by elisa in normal dogs (n = 22), dogs with idiopathic deep pyoderma (n = 22), atopic dermatitis and superficial pyoderma (n = 24), atop ... | 1997 | 9336879 |
| an investigation of phagocytosis and intracellular killing of staphylococcus intermedius by canine neutrophils in vitro. | bacterial infection (pyoderma) of the canine skin is largely caused by staphylococcus intermedius and may be a primary, idiopathic disease or secondary to a range of other dermatological disorders. the immune response to this organism involves phagocytosis and killing by neutrophils. in this study, the effect of opsonisation of s. intermedius by clinical sera (n = 60) and sera from normal dogs (n = 20) was investigated by in vitro phagocytosis and killing assays. all sera from dogs with pyoderma ... | 1997 | 9436266 |
| [deamination of adenine and adenosine in staphylococci]. | deaminations of adenine and adenosine by pattern strains of 24 staphylococcal species, were tested. during 3 hours of incubation of the suspensions of 8 staphylococci with adenine the liberation of ammonia occurred. the same staphylococci accumulated ammonia in the incubation medium with adenosine. the staphylococcus intermedius pcm 2405 strain as opposite to the staphylococcus aureus 536 strain in the media with adenine or adenosine accumulated hypoxanthine or inosine, respectively and ammonia. ... | 1997 | 9554142 |
| prevalence and identity of translocating bacteria in healthy dogs. | bacterial translocation is characterized by the passage of intestinally derived bacteria across the intestinal mucosa to local or regional tissues. this phenomenon is believed to be important in the pathogenesis of gram-negative bacteremia and septicemia; however, the pathway or route of translocation remains unclear. to define the route of translocation better, mesenteric lymph nodes from 50 apparently healthy dogs undergoing elective ovariohysterectomies were cultured aerobically and anaerobic ... | 1997 | 9470154 |
| cytidine catabolism in staphylococci. | products of cytidine catabolism by resting cells of staphylococcus intermedius, staphylococcus xylosus, staphylococcus epidermidis, and staphylococcus saprophyticus were chromatographically separated. under the assay conditions utilized, cytidine was ultimately converted to uracil by the common route in which cytidine was first deaminated to uridine, as well as ammonia, and uridine was cleaved to yield uracil. | 1997 | 9670551 |
| prevention of surgical infections in dogs with a single intravenous injection of marbofloxacin: an experimental model. | eighteen healthy beagle dogs of both sexes were each given 0, 2 or 4 mg/kg marbofloxacin intravenously before the subcutaneous implantation of a silicon tissue cage. two millilitres of a suspension containing 1.3 x 10(4) colony forming units (cfu)/ml of staphylococcus intermedius were then injected into the cage 15 minutes after the intravenous injection. the dogs were clinically assessed immediately, and then two, four, eight and 24 hours after the challenge. samples of inflammatory fluid were ... | 1997 | 9061872 |
| detection and specificity of anti-staphylococcus intermedius secretory iga in human tears. | secretory iga (siga) is the predominant immunoglobulin present in tears that protects the ocular surface against various antigens. staphylococcus intermedius is a member of the normal ocular microbiota. | 1997 | 9267615 |
| organisms isolated from dogs and cats with anaerobic infections and susceptibility to selected antimicrobial agents. | to determine the prevalence of obligate anaerobic bacteria in bacterial infections in dogs and cats and susceptibility to selected antimicrobial agents. | 1997 | 9170087 |
| new combination for the therapy of canine otitis externa. i. microbiology of otitis externa. | in order to compound a new drug combination against canine otitis externa (oe), 515 dogs affected with oe were subjected to physical examination and microbiological analysis of their ear exudates. oe was erythematous-ceruminous in 83 per cent and suppurative in 17 per cent of the patient material. erythematous-ceruminous inflammations were characterised by severe pruritus and accumulation of brownish, greasy cerumen in the auditory canal. the yeast malassezia pachydermatis was isolated from the ... | 1997 | 9065882 |
| nitrate-reducing bacteria on rat tongues. | nitrite-producing bacteria (npb) were isolated from tongues of laboratory rats. the most commonly found nitrite-producing organism was staphylococcus sciuri, followed by staphylococcus intermedius, pasteurella spp., and finally streptococcus spp. both morphometric quantification of bacteria on tongue sections and enumeration of culturable bacteria (cfu) showed an increase in the density of bacteria towards the posterior tongue. up to 65% of bacteria were located in the deep clefts on the posteri ... | 1997 | 9055411 |
| urolithiasis in dogs. iii: prevalence of urinary tract infection and interrelations of infection, age, sex, and mineral composition. | to compile and statistically analyze selected data from a large number of canine urinary calculus specimens that were subjected to quantitative, layer-by-layer mineral analysis. | 1998 | 9582970 |
| urolithiasis in dogs. iv: survey of interrelations among breed, mineral composition, and anatomic location of calculi, and presence of urinary tract infection. | to compile and analyze selected data from a large number of canine urinary calculus specimens that were subjected to quantitative, layer-by-layer mineral analysis. | 1998 | 9582971 |
| a relatively small change in sodium chloride concentration has a strong effect on adhesion of ocular bacteria to contact lenses. | adhesion of bacteria to hydrogel lenses is thought to be an initial step of ocular colonization allowing evasion of normal host defences. the salt concentration of media is an important parameter controlling microbial adhesion. salinity varies from 0.97% nacl equivalents in the open eye to 0.89% in the closed eye state. in this study, the effect of sodium chloride in the concentration range of 0.8-1.0% (w/v) nacl on adhesion of ocular bacteria to soft contact lenses was investigated using a stat ... | 1998 | 9717279 |
| comparison of production of staphylococcus intermedius exotoxin among clinically normal dogs, atopic dogs with recurrent pyoderma, and dogs with a single episode of pyoderma. | to determine whether exotoxin production by staphylococcus intermedius is an important factor in recurrence of pyoderma in atopic dogs. | 1998 | 9676593 |
| elimination of staphylococcus intermedius in healthy dogs by topical treatment with fusidic acid. | cutaneous and mucosal carriage of staphylococcus intermedius was investigated in six healthy beagles before and after application of fusidic acid to mucosal surfaces as 1 per cent viscous eye drops twice daily for seven days. bacterial populations were determined repeatedly over four weeks using quantitative techniques. the overall cutaneous populations of s intermedius reduced significantly (p < 0.001) two days after treatment but returned to pretreatment levels after a further week. the mucosa ... | 1998 | 9693420 |
| identification of staphylococcus species by 16s-23s rdna intergenic spacer pcr analysis. | to investigate whether 16s-23s rdna (rdna) spacer region length polymorphisms are suitable for the identification of staphylococcus strains, the 16s-23s rdna intergenic spacer region lengths of 221 strains belonging to 31 species were studied by using a pcr-based method. each species presented a specific 16s-23s pattern made of 1-8 fragments ranging from 104-771 bp, with the exception of the species staphylococcus warnei, staphylococcus caprae and staphylococcus piscifermentans, which presented ... | 1998 | 9734063 |
| epidemiosurveillance of antimicrobial compound resistance of staphylococcus intermedium clinical isolates from canine pyodermas. | in a retrospective study, 131 staphylococcus intermedius strains isolated from apparently healthy dogs, and 187 staphylococcus intermedius strains isolated from dog pyodermas in the clinical microbiology laboratory at the national veterinary school in nantes, during three successive periods: 1986-87, 1992-93 and 1995-96, were investigated and compared for their antimicrobial susceptibility. results indicated that 60% to 65% of the strains were susceptible to chloramphenicol and doxycyclin, 65% t ... | 1998 | 9611682 |
| microbial flora and antimicrobial susceptibility patterns of isolated pathogens from the horizontal ear canal and middle ear in dogs with otitis media. | to compare microbial flora and antimicrobial susceptibility patterns of isolated pathogens from the horizontal ear canal and middle ear in dogs with otitis media. | 1998 | 9491161 |
| magnetic resonance imaging of presumptive lumbosacral discospondylitis in a dog. | a three-year-old male boxer dog had hyperesthesia, symmetrical epaxial, gluteal and hind limb muscular atrophy and rear limb ataxia. neurological deficits included decreased conscious proprioception of the left hind limb, decreased withdrawal and increased patellar reflexes of both hind limbs. the dog had a urinary tract infection with positive culture for staphylococcus intermedius. on survey radiography of the lumbosacral spine there was active bone proliferation spanning the l7 s1 interverteb ... | 1998 | 9491511 |
| [pore-forming leukotoxins from staphylococcus aureus: variability of the target cells and 2 pharmacological processes]. | the staphylococcal bi-component leukotoxins constitute a family included in the super-family of the beta-sheet-structured pore-forming toxins. they may be produced by staphylococcus aureus and by staphylococcus intermedius and their target cells vary according to the molecules. the mode of action proceeds by the sequential binding of the class s proteins, then by that of the class f proteins at the surface of the membranes. then, the activation of cellular calcium-channels precedes the pore form ... | 1998 | 9769878 |
| coagulase-negative staphylococcus intermedius isolated from milk from dairy cows in israel. | 1998 | 9854775 | |
| determination of skin concentrations of enrofloxacin in dogs with pyoderma. | to compare serum and skin concentrations of enrofloxacin in dogs with pyoderma with those of clinically normal dogs and to evaluate concentrations in dogs with superficial versus deep pyoderma. | 1998 | 9858413 |
| efficacy of clindamycin hydrochloride capsules for the treatment of deep pyoderma due to staphylococcus intermedius infection in dogs. | clindamycin hydrochloride capsules (11 mg/kg body weight, q24 h) were administered orally to 20 dogs with deep staphylococcal pyoderma. response to therapy was excellent in 100% of the dogs. duration of therapy varied from 21 to 91 d, with an average duration of 45 d. relapses occurred in 25% of the dogs within a 3-month period. one dog vomited when the clindamycin was given on an empty stomach. under the conditions of the study, clindamycin was an effective, safe, and convenient antibiotic for ... | 1998 | 9861499 |
| molecular genotyping of staphylococcus aureus strains: comparison of repetitive element sequence-based pcr with various typing methods and isolation of a novel epidemicity marker. | repetitive sequence-based (rep)-pcr genotyping as described here is based on the presence of homologues of mycoplasma pneumoniae repeat-like elements in staphylococcus. in this study we comparatively evaluated the usefulness of rep-pcr typing with two sets of well-defined collections of staphylococcus aureus strains. rep-pcr analysis of the first collection of s. aureus strains (n = 59) and one staphylococcus intermedius strain showed 14 different rep-pcr patterns, with each pattern harboring 6 ... | 1999 | 9889215 |
| vertebral physitis with epiphyseal sequestration and a portosystemic shunt in a pekingese dog. | vertebral physitis with bone sequestration and a portosystemic shunt were diagnosed in an 18-month-old female pekingese dog. the latter was determined by the presence of low blood urea nitrogen, elevated serum bile acids, microhepatica and an increased portosystemic shunt fraction. it was managed with a home-cooked low protein diet. vertebral physitis and bone sequestration was diagnosed by the presence of thoracolumbar hyperaesthesia, radiographic and scintigraphic changes, isolation of staphyl ... | 1999 | 10649596 |
| activity of chlorhexidine shampoos in vitro against staphylococcus intermedius, pseudomonas aeruginosa and malassezia pachydermatis. | 1999 | 10378283 | |
| therapy of difficult cases of canine pyoderma with marbofloxacin: a report of 39 dogs. | thirty-nine dogs with severe and/or recurrent lesions of pyoderma were treated with marbofloxacin at an average dosage of 2.12 mg/kg bodyweight, once daily, for time periods varing from 10 to 213 days. forty-seven strains of bacteria, isolated from 34 cultures, were tested for sensitivity to various antibiotics. at day 0, no resistance to marbofloxacin was found, but one refractory case, a strain of staphylococcus intermedius resistant to marbofloxacin, was cultured at day 28. thirty-three dogs ... | 1999 | 10404486 |
| staphylococcus intermedius cellulitis and toxic shock in a dog. | a labrador retriever was examined for sudden lameness and cellulitis of the right forelimb. bacterial culture of the dermis yielded a large number of staphylococcus intermedius. the association of this bacterium with toxic shock is discussed. | 1999 | 10416072 |
| identification of coagulase-positive staphylococci isolated from bovine milk. | a total of 414 coagulase-positive staphylococcal strains obtained at the mastitis laboratory, national veterinary institute, uppsala, sweden, were studied. one hundred and seventy seven strains were used for a frequency study. ninety-seven per cent were identified as staphylococcus aureus, 2% as staphylococcus intermedius and 1% as staphylococcus hyicus. two hundred and thirty seven strains with atypical hemolysis reactions on bovine blood agar were randomly selected, with the aim to increase th ... | 1999 | 10918901 |
| methicillin-resistant staphylococcus intermedius pneumonia following coronary artery bypass grafting. | 1999 | 10433599 | |
| catabolism of the methyl derivatives of adenosine and cytidine in staphylococci. | products of 1-methyladenosine, 2'-o-methyladenosine, 2'-o-methylcytidine, and 5-methylcytidine catabolism by resting cells of staphylococcus aureus and staphylococcus intermedius were chromatographically separated. the methyl group in 1-methyladenosine protected the adenosine derivative from deamination by s. intermedius but it did not protect n-glycosidic bond from cleavage by s. intermedius and s. aureus. the methyl group in 2'-o-methyladenosine and 2'-o-methylcytidine protected the n-glycosid ... | 1999 | 10464951 |
| methicillin resistance among staphylococci isolated from dogs. | to determine whether methicillin-resistant staphylococci from dogs expressed the meca gene and to determine what proportion of canine staphylococcal isolates positive for the meca gene were resistant to oxacillin and other antibiotics. | 1999 | 10622162 |
| identification of bacterial species associated with the sheep scab mite (psoroptes ovis) by using amplified genes coding for 16s rrna. | this was the first molecular study of the bacterial flora of the sheep scab mite (psoroptes ovis). a sequence analysis of genes coding for 16s rrna revealed that serratia marcescens and bacteria closely related to staphylococcus intermedius or staphylococcus chromogens and alloiococcus otitidis were present. these bacteria were associated with skin lesions, dermatitis, and otitis media caused by p. ovis. | 1999 | 10473440 |
| in vitro activity of difloxacin against canine bacterial isolates. | the in vitro activity of difloxacin against canine bacterial isolates from clinical cases was studied in the united states and the netherlands. minimal inhibitory concentrations (mic), the postantibiotic effect, the effect of ph on antimicrobial activity, and the bacterial killing rate tests were determined according to standard techniques. the mics of american and dutch isolates agreed in general. the mics of the american gram-negative isolates ranged from 0.06 to 2.0 microg/ml, and the mics of ... | 2000 | 10826834 |
| use of multilocus enzyme electrophoresis to distinguish clinically important strains of staphylococcus intermedius from the skin of dogs. | to use multilocus enzyme electrophoresis to determine the genetic structure of staphylococcus intermedius from normal skin of dogs and those isolated from a variety of disease conditions and to distinguish clinically important strains in dogs. | 2000 | 10840575 |
| molecular phylogenetic evidence for noninvasive zoonotic transmission of staphylococcus intermedius from a canine pet to a human. | rrna-based molecular phylogenetic techniques were used to identify the bacterial species present in the ear fluid from a female patient with otitis externa. we report the identification of staphylococcus intermedius from the patient and a possible route of transmission. analysis of 16s ribosomal dna restriction fragment length polymorphisms indicated that the dominant species present was s. intermedius. a pet dog owned by the patient also was tested and found to harbor s. intermedius. in humans, ... | 2000 | 10747154 |
| molecular analysis of the translational attenuator of a constitutively expressed erm(a) gene from staphylococcus intermedius. | during the course of a study on macrolide, lincosamide and streptogramin b resistance among staphylococci from animal sources, a staphylococcus intermedius isolate was found to carry a constitutively expressed erm(a) gene on the 70 kb plasmid pses29. the molecular basis of constitutive erm(a) expression was investigated by cloning and sequence analysis of the erm(a)-associated translational attenuator. two point mutations in this regulatory region were detected. these mutations cause constitutiv ... | 2000 | 11062198 |
| adherence by staphylococcus intermedius to canine keratinocytes in atopic dermatitis. | the adherence of staphylococcus intermedius to canine keratinocytes in normal dogs was compared to that in dogs suffering from atopic dermatitis, primary seborrhoea and bacterial pyoderma. statistically significant greater adherence by s. intermedius to keratinocytes occurred in atopic dogs and dogs suffering from pyoderma when compared with the normal group (p < 0.01) and dogs suffering from primary seborrhoea (p < 0.05). this is similar to the results of a study of human atopic dermatitis by c ... | 2000 | 10877976 |
| left ventricular outflow tract to left atrial fistula associated with endocarditis in a dog. | a one-year-old, intact male, 28-kg, mixed-breed dog developed neurological episodes consistent with emboli. an acquired iii/vi holosystolic heart murmur was ausculted in the mitral area, and valvular endocarditis with pulmonic and aortic insufficiency were noted at echocardiographic examination. an abnormal communication (i.e., fistula) between the left ventricular outflow tract (lvot) and the left atrium adjacent to the mitral valve annulus was noted with doppler imaging and confirmed with angi ... | 2000 | 10730623 |
| [interspecies lysogenization in staphylococci: transfer of bacteriophage converting enterotoxin a from a clinical strain of staphylococcus aureus to staphylococcus intermedius]. | the ability of lysogenization was examined of 50 s. intermedius strains and of 77 strains belonging to 14 different species of coagulase-negative staphylococci using 8 enterotoxin a converting bacteriophages isolated from s. aureus. all the examined bacteriophages showed lytic activity against at least 1 of 11 susceptible strains of s. intermedius to them. lytic activity towards coagulase-negative staphylococci was observed for 6 of 8 examined bacteriophages. two bacteriophages were active again ... | 2000 | 11286173 |
| evaluation in vitro of the antimicrobial activity of two topical preparations used in the management of ear infections in the dog. | products designed for ear cleansing (i.e., epi-otic and epi-otic new formula [nf]a) were evaluated in vitro for their ability to kill clinical isolates of staphylococcus intermedius, pseudomonas aeruginosa, and the yeast malassezia pachydermatis. dilutions of the products at 1/5 and 1/25 in distilled water were inoculated with suspensions of the organisms and held at room temperature. the mixtures were sampled at intervals between 1 and 16 minutes, and numbers of viable colony-forming units of t ... | 2000 | 19757564 |
| evaluation of in vitro activity of two topical products against three organisms isolated from canine referral patients with otitis externa and cutaneous pyoderma. | canine otitis externa and cutaneous pyoderma are common problems that are often associated with staphylococcus intermedius, pseudomonas aeruginosa, and malassezia pachydermatis. in vitro activity of two topical products against these organisms isolated from canine referral patients were evaluated. organisms were grown and diluted to a concentration equivalent to 10(7) colony-forming units (cfu) per ml and exposed to either a 0 or 1/5 dilution of hexadene flush with spherulites (virbac animal hea ... | 2000 | 19757573 |
| the value of rrna gene restriction site polymorphism analysis for delineating taxa in the genus staphylococcus. | a total of 101 staphylococcal strains were ribotyped using ecori and hindiii as restriction enzymes and plasmid pba2 as the rdna probe. isolates from 10 newly described staphylococcal taxa were among those examined. all the ribotypes were added to our database, staph db, which now contains the sizes of the bands of 135 ecori and 120 hindiii ribotypes from 408 strains belonging to 42 staphylococcal taxa. the relatedness of ribotypes was evaluated by using the dice coefficient. the ribotypes, and ... | 2000 | 10758876 |
| characterization of coagulase from staphylococcus intermedius. | a protein coagulase was isolated from staphylococcus intermedius 6131 using bovine prothrombin-sepharose 4b and bio-gel p-4 column chromatographies. homogeneity was demonstrated by the formation of a single band in polyacrylamide gel electrophoresis and isoelectric focusing. the purified preparation possesses a molecular weight of 64,500, an isoelectric point of 4.1, consists of 615 total amino acid residues and demonstrates coagulase activity for human and rabbit fibrinogen, but does not show t ... | 2001 | 11405274 |
| evaluation of the clinical efficacy of marbofloxacin (zeniquin) tablets for the treatment of canine pyoderma: an open clinical trial. | the efficacy and field safety of marbofloxacin (zeniquin) for the treatment of superficial and deep bacterial pyoderma were evaluated. seventy-two dogs were treated with 2.75 mg kg-1 of marbofloxacin orally once daily for 21 or 28 days. sixty-two dogs (86%) had superficial pyoderma and 10 (14%) had deep pyoderma. a history of prior pyoderma was reported in 39/72 dogs. pretreatment aerobic bacteriologic cultures of skin lesions were performed in 47 cases and the predominant pathogen isolated was ... | 2001 | 11420932 |
| antimicrobial activity of enrofloxacin against staphylococcus intermedius strains isolated from canine pyodermas. | this study examined and compared the minimal inhibition concentrations (mics) of enrofloxacin against 393 staphylococcus intermedius strains isolated in france from canine pyodermas during three different years, 1995 (174 isolates), 1997 (101 isolates) and 1999 (118 isolates). the mics of enrofloxacin against these strains ranged from 0.063 to 64 mg l-1, with mic50 and mic90 equal to 0.125 and 0.25 mg l-1, respectively. two resistant strains were found, but only among isolates collected in 1999. ... | 2001 | 11420933 |
| dog-bite wounds: bacteriology and treatment outcome in 37 cases. | bite wounds in 37 dogs were prospectively evaluated. ninety-five percent of animals presented within 12 hours of injury. the most common wound locations were neck, limbs, head, chest, shoulder region, and abdomen. eighty-six percent had wounds to multiple locations. fifty-seven percent of wounds were class 4 (i.e., most severe). based on results of all samples, 65% had positive aerobic cultures, 15% had positive anaerobic cultures, and 33% had negative cultures. the most commonly isolated aerobi ... | 2001 | 11563444 |
| enterotoxigenic potential of staphylococcus intermedius. | staphylococcal food poisoning (sfp) caused by enterotoxigenic staphylococci is one of the main food-borne diseases. in contrast to staphylococcus aureus, a systematic screening for the enterotoxins has not yet been performed on the genomic level for the coagulase-positive species s. intermedius. therefore, the enterotoxigenic potential of 281 different veterinary (canine, n = 247; equine, n = 23; feline, n = 9; other, n = 2) and 11 human isolates of s. intermedius was tested by using a multiplex ... | 2001 | 11722906 |
| comparative ribotyping of staphylococcus intermedius isolated from members of the canoidea gives possible evidence for host-specificity and co-evolution of bacteria and hosts. | a total of 41 staphylococcus intermedius isolates were isolated from skin of healthy members of six phylogenetic groups within the canoidea (the dog family, skunk subfamily, weasel subfamily, racoon family, red panda and bear family) of different geographical origin and compared by ecori ribotyping and cluster analysis. the s. intermedius isolates from the different families and subfamilies clustered together in separate groups, almost completely following the phylogenetic relationship of the an ... | 2001 | 11491331 |
| antimicrobial susceptibility of staphylococcus intermedius isolated from healthy and diseased dogs. | a total of 90 strains of staphylococcus intermedius isolated from dogs were examined for antimicrobial susceptibility. there were no significant differences in the distribution patterns of mics between strains from 1982 to 1985 and those from 1999, and between strains from healthy dogs and those from diseased dogs. all of the strains were susceptible to abpc, dmppc, cex, tdm, erfx, bflx, and ff at concentrations of 0.05 to 6.25 microg/ml. the mics of otc, km, em, aiv-ts, and lcm were distributed ... | 2001 | 11307945 |
| molecular epidemiology and genetic linkage of macrolide and aminoglycoside resistance in staphylococcus intermedius of canine origin. | a collection of 77 staphylococcus intermedius isolates from dogs and cats in switzerland was examined for resistance to erythromycin. resistance profiles for 14 additional antibiotics were compared between erythromycin-resistant and susceptible isolates. a resistance prevalence of 27% for erythromycin was observed in the population under study. complete correlation between resistance to erythromycin, and to spiramycin, streptomycin, and neomycin was observed. the erythromycin-resistant isolates ... | 2001 | 11230937 |
| antimicrobial resistance in staphylococci from animals with particular reference to bovine staphylococcus aureus, porcine staphylococcus hyicus, and canine staphylococcus intermedius. | besides their role as commensals on the skin and mucosal surfaces, staphylococci may be involved in a wide variety of diseases in animals. staphylococcal infections in animals are mainly treated with antimicrobial agents and as a consequence, staphylococci from animal sources have developed and/or acquired resistance to the respective antimicrobial agents. resistance statistics obtained from national monitoring programmes on staphylococci from cattle and pigs, but also from surveillance studies ... | 2001 | 11432424 |
| transmission of opportunistic pathogens in a veterinary teaching hospital. | acinetobacter baumannii, enterococcus faecalis, enterococcus faecium, and staphylococcus intermedius isolates from infected surgical wounds and other types of infections in a veterinary teaching hospital were typed by pulsed field gel electrophoresis. a first cluster of infections with a multiresistant a. baumannii strain was observed in dogs and cats in 1998-1999. this strain disappeared after cleaning and disinfection of the companion animals intensive care unit. it was followed in 2000 by a s ... | 2001 | 11506928 |
| [the current antimicrobial resistance situation in swiss veterinary medicine]. | antimicrobial susceptibility data (n = 1501) and bacterial isolates (n = 258) of important bacterial pathogens from animals were collected in collaboration with eight swiss laboratories from may 1999 to february 2000. using these data, the antimicrobial resistance situation could be assessed for the following bacterial species: escherichia coli, salmonella, haemophilus parasuis, actinobacillus pleuropneumoniae, pasteurella multocida, mannheimia haemolytica, bordetella bronchiseptica, campylobact ... | 2001 | 11680911 |
| clinical prevalence and antimicrobial susceptibility of staphylococcus aureus and staph. intermedius in dogs. | this study was undertaken to investigate whether the antibiotic resistance of staphylococcus aureus and staph. intermedius varies with the site of isolation, sex or age of dogs. | 2002 | 12174038 |
| frequency of isolation and antimicrobial susceptibility patterns of staphylococcus intermedius and pseudomonas aeruginosa isolates from canine skin and ear samples over a 6-year period (1992-1997). | staphylococcus intermedius (s. intermedius) was isolated from 88.6% and 49.4% of skin and ear samples, respectively, during the years 1992 through 1997, and frequency of isolation remained unchanged. more than 95% of all s. intermedius isolates were susceptible to cephalothin and oxacillin, providing support for empirical treatment of canine skin and ear infections with cephalexin. pseudomonas aeruginosa (p. aeruginosa) was isolated from 7.5% and 27.8% of skin and ear samples, respectively. the ... | 2002 | 12220023 |
| use of computerized image analysis to quantify staphylococcal adhesion to canine corneocytes: does breed and body site have any relevance to the pathogenesis of pyoderma? | an optimized system of computerized image analysis was used to investigate variations in the adherence of staphylococcus intermedius to canine corneocytes from four different breed groups and six different anatomical sites. s. intermedius showed significantly greater adherence to the head and neck compared with the dorsum, but adherence to the limb, axilla and groin did not differ from other sites. furthermore, there was significantly greater adherence of s. intermedius to corneocytes from the d ... | 2002 | 11896968 |
| rpob gene sequence-based identification of staphylococcus species. | the complete sequence of rpob, the gene encoding the beta subunit of rna polymerase was determined for staphylococcus saccharolyticus, staphylococcus lugdunensis, s taphylococcus caprae, and staphylococcus intermedius and partial sequences were obtained for an additional 27 staphylococcus species. the complete rpob sequences varied in length from 3,452 to 3,845 bp and had a 36.8 to 39.2% gc content. the partial sequences had 71.6 to 93.6% interspecies homology and exhibited a 0.08 to 0.8% intras ... | 2002 | 11923353 |
| bacterial colonization of intravenous catheters in young dogs suspected to have parvoviral enteritis. | to determine the prevalence of bacterial colonization of iv catheters among young dogs suspected to have parvoviral enteritis, to identify the organisms responsible for catheter colonization, and to determine the antimicrobial susceptibility of organisms that were obtained. | 2002 | 11991409 |
| identification of serine138 residue in the 4-residue segment k135k1361137s138 of luks-i component of staphylococcus intermedius leukocidin crucial for the luks-i-specific function of staphylococcal leukocidin. | luk-i produced by staphylococcus intermedius was found to be a new member of the staphylococcal bi-component pore-forming toxin family, in which staphylococcal leukocidin, panton-valentine leukocidin, and gamma-hemolysin are included. luk-i consists of luks-i and lukf-i. from the deduced amino acid sequence of luks-i, a 4-residue sequence, k135k1361137s138, at the root of the stem region was found to be identical with that of the phosphorylated segment of a protein phosphorylated by protein kina ... | 2002 | 11999405 |
| characterization of staphylococcus intermedius from pigeons, dogs, foxes, mink, and horses by pulsed-field gel electrophoresis. | staphylococcus intermedius from pigeons, dogs, foxes, mink, and horses, was characterized by pulsed-field gel electrophoresis (pfge) to evaluate the use of this typing method for discriminating among strains. smai cut the chromosomal dna into 7-13 fragments ranging from approximately 48 kb to 655 kb, with most of the detectable fragments being smaller than 172 kb. s. intermedius from various animals had a high degree of restriction fragment length polymorphism. pigeon strains have a similar geno ... | 2002 | 11999443 |
| population diversity of staphylococcus intermedius isolates from various host species: typing by 16s-23s intergenic ribosomal dna spacer polymorphism analysis. | twelve 16s-23s ribosomal dna intergenic spacer (its-pcr) types were identified among 57 staphylococcus intermedius isolates from humans and other animals. six its-pcr types were host specific, and most human and canine strains belonged to the same types (a and j). pigeon, horse, and mink strains appeared more heterogeneous. | 2002 | 12037109 |
| colonization of neonatal puppies by staphylococcus intermedius. | cutaneous and mucosal carriage of staphylococcus intermedius was determined in five cavalier king charles spaniel bitches, living in normal domestic environment, and their 18 puppies during the immediate prepartum period and after whelping, over a total of 8 weeks, using quantitative swab techniques. within 8 h of birth s. intermedius was isolated from each puppy. the nose and mouth were the most frequently colonized mucosal sites (83 and 78%, respectively). at that time s. intermedius was isola ... | 2002 | 12074701 |
| pathological and clinical aspects of the diseases caused by malassezia species. | from veterinary point of view malassezia pachydermatis has the greatest significance. it has been standing in the focus of interest since the early 1990s, mostly because of the frequency of otitis externa and dermatitis caused by this yeast in dogs. this is the only lipid-independent species in the genus malassezia. it can be found in very large proportion on the skin of healthy animals, but can be isolated in much greater number from diseased dogs. it often causes illness together with other pa ... | 2002 | 12109170 |
| adherence of staphylococcus intermedius to canine corneocytes in vitro. | this study investigated the in vitro adherence of staphylococcus intermedius to canine corneocytes, collected from a healthy dog using double-sided adhesive tape. adherence was shown to depend on duration (p < 0.001) and temperature of incubation (p < 0.001) and the concentration of bacteria (p < 0.001). isolates of s. intermedius from lesions of pyoderma were not generally more adherent to healthy canine skin than were isolates from healthy dogs. significant differences in adherence were demons ... | 2002 | 12174179 |
| development of a pcr test for the identification of staphylococcus intermedius based on the 16s rdna sequence. | the development of a pcr assay based on the 16s ribosomal rna gene (rdna) sequence was carried out for the identification of staphylococcus intermedius. sixty-six strains of s. intermedius, 70 of staphylococcus aureus and 2 of staphylococcus hyicus were examined for the assay. the 16s rdna, of which the pcr target fragment makes up 901 bp corresponding to the sequence data of the gene, was detected in all strains of s. intermedius, but it was not detected in any strains of either s. aureus or s. ... | 2002 | 12185314 |
| intra-abdominal botryomycosis in a dog. | a 6-year-old 41.8-kg (92-lb) sexually intact male german shepherd dog used as an attack and pursuit dog by the local police department was examined because of general malaise and sudden onset of ataxia. abnormal findings included a high wbc count, fever, and ongoing weight loss. physical examination was unrevealing initially, in part because of the aggressive nature of the dog. following treatment with antibiotics, an abdominal mass was detected during a second physical examination, and was conf ... | 2002 | 12420781 |
| frequency of superantigen-producing staphylococcus intermedius isolates from canine pyoderma and proliferation-inducing potential of superantigens in dogs. | this preliminary study investigated the potential role of staphylococcal superantigens in the pathogenesis of canine pyoderma. the staphylococcal enterotoxins a (sea), seb, sec and sed, and the toxic shock syndrome toxin-1 (tsst-1) were assayed in isolates from skins of dogs with pyoderma. culture supernatants from 25 of 96 isolates were positive for multiple superantigens, with sea and sec being the most frequently detected. in in vitro stimulation of canine peripheral blood mononuclear cells a ... | 2002 | 12443685 |
| detection of secreted peptides by using hypothesis-driven multistage mass spectrometry. | a method is presented for the rapid detection and characterization of trace amounts of peptides secreted from microorganisms, including pheromones, virulence factors, and quorum-sensing peptides. the procedure, based on targeted multistage ms, uses a novel matrix-assisted laser desorptionionization-ion trap mass spectrometer to overcome limitations of current ms methods (limited dynamic range, signal suppression effects, and chemical noise) that impair observation of low abundance peptides from ... | 2003 | 12591958 |
| assessment of the aerobic faecal microflora in mink (mustela vison schreiber) with emphasis on escherichia coli and staphylococcus intermedius. | the present study was undertaken to investigate the culturable aerobic faecal microflora of mink from newborn until adulthood with emphasis on the potential pathogens escherichia coli and beta-haemolytic coagulase positive staphylococci. rectal swabs were taken from 10 healthy dams and their offspring on seven mink farms throughout the production season and a semi-quantitative enumeration of total e. coli and haemolytic e. coli, beta-haemolytic streptococci, beta-haemolytic coagulase positive st ... | 2003 | 12695047 |
| isolation of exfoliative toxin from staphylococcus intermedius and its local toxicity in dogs. | a rounding effect was demonstrated in cultured cells inoculated with the culture filtrates (cfs) of 60 strains of staphylococcus intermedius derived from dogs affected with pyoderma. exfoliative toxin (et)-like toxin (etlt) was isolated from the cf of s. intermedius strain d-52, which exhibited strong rounding activity and then was purified by gel filtration on a sephadex g-75 column, and by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). the etlt caused exfoliation in 1-da ... | 2003 | 12742712 |
| cloning of the gene coding for staphylococcus intermedius exfoliative toxin and its expression in escherichia coli. | an exfoliative toxin (siet)-producing strain (d-52) of staphylococcus intermedius derived from canine pyoderma did not possess large plasmids. therefore, the gene coding for siet was considered to be located on the chromosomal dna. the siet gene was cloned from the chromosomal dna of s. intermedius and was expressed in escherichia coli. the nucleotide sequence of the siet gene consists of a coding region of 990 bp specifying a polypeptide of 330 amino acid residues, which included a putative 42- ... | 2003 | 12742713 |