Publications

change in the ratio of cytochrome oxidase activity to nitrite reductase activity of pseudomonas aeruginosa nitrite reductase with the kind of c-type cytochrome used as an electron donor.	the ratio between the nitrite reductase and cytochrome oxidase activities of pseudomonas aeruginosa nitrite reductase [ec 1.9.3.2.] varies with kind of c-type cytochrome used as the electron donor. withe cytochrome c-548, 554 (micrococcus sp.), the nitrite reductase activity is greater than the cytochrome oxidase activity, while the former is smaller than the latter with cytochrome c-554 (navicula pelliculosa). the aerobic oxidation catalyzed by this enzyme of denitrifying bacterial ferrocytoch ...	1976	178646
[effect of polyakov's "prints" on the catalytic properties of an enzyme substrate system].	silicagels, formed in presence of an enzyme (histidine decarboxylase from micrococcus sp. n.), substrate (l-histidine-hcl-h2o) and inhibitor (imidazole) (so-called polyakov's "print") exhibited specific catalytic properties in the reaction of l-histidine decarboxylation. the "prints" of the enzyme, inhibitor and substrate increased the activity of the enzyme-substrate system studied. the increased activity was probably due to the specific adsorption of imidazole ring of the substrate molecule by ...	1975	175565
the functional tryptophan and tyrosine residues of f1 atpase of micrococcus sp. atcc 398.		1978	146676
f1-atpase from micrococcus sp. atcc 398. purification by ion-exchange chromatography and further characterization. (auto)proteolysis and dissociative effects.	the preparation of highly purified f1-atpase from micrococcus sp. atcc 398 by application of deae-sepharose cl-6b chromatography as final step is described. this enzyme consists of five subunits of different molecular weight: alpha (65000), beta (55000),gamma (35000), delta (20000), and epsilon (17000). disc electrophoresis on 5% polyacrylamide gels removes the epsilon-polypeptide yielding an active atpase complex with four different subunits: alpha, beta, gamma, delta. additionally, by variatio ...	1977	145365
membrane-bound f1 atpase from micrococcus sp. atcc 398e. purificationa and characterization ny affinity chromatography.	a chemically reactive atp analogue, 6-[(3-carboxy-4-nitrophenyl)thio]-9-beta-d-ribofuranosylpurine 5'-triphosphate (nbs6itp) has been synthesized. it has the ability to form stable thioether bonds between the 6-position of the purine ring and aliphatic mercapto groups. the nucleotide moiety of the reagent has been covalently bound to agarose, via iminobispropylamine and n-acetyl-homocysteine as space with the purpose of producing an affinity chromatography material. the affinity matrix binds sol ...	1976	134891
me2+-(13 s) atpase from micrococcus sp. atcc 398e. the effect of trypsin on the purified enzyme.	by trypsin treatment of highly purified atpase (ec 3.6.1.3) from micrococcus sp. atcc 398e, two enzyme modifications have been obtained. (i) atpase ta, which has about the same activity as untreated atpase. (ii) a protein complex ti, which lacks atpase activity, but nevertheless binds atp as shown by affinity chromatography. trypsin primarily shortens the alpha-chains of the "native" enzyme to alpha-chains and removes the gamma-subunit, thus yielding atpase ta. the formation of the protein compl ...	1976	131581
microbial formation and degradation of dimethylamine.	dimethylamine was formed from trimethylamine in soils of different ph values. the rate of disappearance of the secondary amine from soil was affected by ph and was markedly reduced under anaerobiosis. the accumulation of dimethylamine in cultures of micrococcus sp. provided with trimethylamine depended on the nitrogen sources available to the bacterium but was not greatly influenced by the c-n ratio of the medium. dimethylamine and nitrite accumulated in large amounts at ph 6.0 to 8.0 in culture ...	1976	7191
study of the structure of the histidine decarboxylase of micrococcus sp. n. by the method of circular dichroism.	ring dichroism spectra (rd) of histidine decarboxylase (hdc) from micrococcus sp. n. at the regions of peptide bonds (200-240 nm) and aromatic amino acids (250-300 nm) absorption are studied. the treatment of rd spectra according to methods of greenfield-fasman, saksena-vetlaufer and mayer permits to conclude that at the ph range within 4-8 the content of ordered structures of alpha-helix type comprises 20%, that of beta-structure type-40%, while the rest 40% are represented with polypeptide cha ...	1976	6076
flourescent properties of histidine decarboxylase from micrococcus sp. n.	a dependency of fluorescence parameters of histidinedecarboxylase (hdc) from micrococcuc sp. n. on ph values is studied. native hds has a short-waved maximum position (325 nm) and a small half-width of the fluorescence spectrum (48nm). the change in the quantum yield of the enzyme fluorescence was parallel with the change of the enzymatic activity. triptophane residues of native hdc are located at hydrophobic region of the enzyme globula. the dependency of hdc flourescence parameters on ph value ...	1975	1114