Publications

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[the effect of modifications of the "penam" part of penicillin o]. 1976826035
respiratory activity as a determinant of radiation survival response.respiration is depressed in irradiated bacteria reaching a minimum level in most strains at 1-3 h after exposure when incubated in growth medium. since a delay in response is observed, direct action on respiratory enzymes is unlikely. the dosage response of respiration varies widely in the strains studied with do's from 15 kr for escherichia coli bs-1 to 1200 kr for micrococcus radiodurans. all strains exhibit two-component dosage-response curves. escherichia coli bs-1 uniquely possesses a posit ...1976788873
concentrations of erythromycin in serum and tonsil: comparison of the estolate and ethyl succinate suspensions.concentrations of erythromycin were measured in serum and tonsil from children who had received either the estolate or ethyl succinate suspension before surgery. the in vitro assay measured total erythromycin activity against a group a beta hemolytic streptococcus. levels of erythromycin in serum and tonsil after single and multiple doses of the estolate were significantly higher than those after administration of the ethyl succinate. the therapeutic implications of these findings are unknown.1976792404
pharmacokinetic interpretation of erythromycin and tylosin activity in serum after intravenous administration of a single dose to cows.the distribution and elimination kinetics of erythromycin and tylosin, which are macrolide antibiotics, were studied in healthy cows. a single dose (12-5 mg/kg) of drug was administered as an intravenous bolus, and blood samples were collected at precisely timed intervals. the standard cylinder plate bioassay method using sarcina lutea as test organism was employed to determine antibiotic activity in the serum. the results suggested that these drugs are distributed in at least two kinetically di ...19761030820
penicillin tolerance in neisseria gonorrhoeae: evidence disallowing a penicillinase-mediated mechanism from a refined microbiological assay method.a microbiological assay method has been developed and applied to neisseria gonorrhoeae, for the purpose of detecting enzymatic deactivation of benzyl penicillin. calibration of the method, using strains of escherichia coli k-12 with previously reported penicillinase (ec 3.5.2.6.) activities, has shown that it is extremely sensitive and may be used in a quantitative manner. at the limit of sensitivity the test is able to detect penicillin breakdown in the order of 3 x 10(-3) mug in 48 h, which is ...1976814985
quantitative structure-activity relationships. 2. a mixed approach, based on hansch and free-wilson analysis.based on the theoretical and numerical equivalence of hansch's linear multiple regression model and the modified free-wilson model a mixed approach is developed. the mixed approach is a combination of both models which makes use of the advantages of each model and widens the applicability of hansch and free-wilson analysis. the free-wilson approach now is applicable also in the case of parabolic dependence of biological activity on a particular physical property, e.g., log p or pi. a rational ex ...1976818381
[experimental study of an aerosol preparation containing gentamicin (gentasol)].composition of a suspension aerosol drug, gentasol for local application was developed. it contained gentamycin and prednisolon. the antimicrobial properties of the drug were studied in vitro. it was found that gentasol had a pronounced antimicrobiol effect and inhibited the growth of many microorganisms including cultures resistant to other antibiotics. the drug was active against ps. aeruginosa and proteus, which is an advantage of it as compared to other antibiotic aerosol drugs. the study sh ...1976818945
[drug residues in poultry (author's transl)].the following microbiological tests: the bacillus subtilis bga (bundes-gesundheitsamt) test and sarcina lutea test as well as the test bacteria bacillus stearothermophilus var. calidolactis and e. coli were used to examine whether drug residues were detectable in broiler chickens during and after treatment with various drugs. with the exception of the occidiostat esb3, residues were not found to be present using the two above tests. on the other hand, residues of a number of drugs were detected ...1976821173
antibiotic residues and their recovery from animal tissues. 1976821907
determination of protein in preparations of microsomes. 19751130662
synthesis and antitumor activity of 2-deamino- and n2-(gamma-hydroxypropyl)actinomycin d.2-deamino- and n2-(gamma-hydroxypropyl)actinomycin d were synthesized by modification of the parent actinomycin d molecule at the 2 position of the phenoxazinone moiety. the common intermediate was 2-deamino-2-chloroactinomycin d. catalytic hydrogenation of this material afforded the 2-deamino derivative while treatment with gamma-hydroxypropylamine yielded the n2-(gamma-hydroxypropyl) derivative. these 2-substituted actinomycin d derivatives were less potent in microbiological assays than the p ...1975809581
[false-positive results obtained on examining slaughtered animals for the presence of antibiotic residues (author's transl)].as part of the examination of emergency-slaughtered animals for the presence of antibiotic residues, studies were done to see whether false-positive results would be obtained when the sarcina lutea kidney test and bacillus subtilis bga test were performed. when the s. lutea kidney test was positive in cattle, calves and swine, penicillin was invariably found to be present in those animals, the histories of which showed that they had not been given antibiotics. a syringe and an injected fluid con ...1975809859
[transfer of organisms during exchange of heat and moisture in air conditioning installations (author's transl)].with the exhaust air from ventilation and air conditioning installations escaping into the open, the heat content is also lost which fresh air from outside obtains at considerable expense of energy and technical equipment. the heat content, on the other hand, consists of about equal proportions of sensible heat and latent heat which is associated with the moisture content of the air. in order to regain the heat content of the escaping air so as to be able to use it again - and this is becoming i ...1975811009
[residues of antimicrobial substances in the meat and organs of slaughter calves].using a modified gartside diffusion method (1960) the incidence of the residues of antimicrobial substances was followed in two categories of calves commercially slaughtered at the brno slaughter house. for testing the following three collection strains were used: b. subtilis (atcc 6633), b cereus, var. mycoides (atcc 11778), sarcina lutea (atcc 9341). in light weight calves samples of bile, liver, kidney, diaphragmatic and hind limb muscles, in heavy calves, due to technical reasons, only bile, ...1975807014
systematic difference in the methylation of ribosomal ribonucleic acid from gram-positive and gram-negative bacteria.a survey of gram-positive and gram-negative organisms was performed to compare the distributionof n6-methylated adenine. it was found that (i) all the gram-positive strains tested, staphylococcus aureus, sarcina lutea, bacillus stearothermophilus, bacillus subtilis, and bacillus megaterium, contain neither n6-monomethyl adenine (m6a) nor n6-dimethyladenine (m26a) in 23s ribosomal ribonucleic acid (rrna). in the case of s. aureus and streptococcus pyogenes, strains which are clinically resistant ...1975807565
synthesis and antibacterial and antifungal activities of 5-nitro-2-furfurylidene polyhalophenoxyacethydrazides viii.fifteen 5-nitro-2-furfurylidene phenoxyacethydrazides were synthesized, and the antibacterial and antifungal activities of the compounds prepared were determined against different microorganisms. the o-methoxy derivative was found to be the most active compound.1975807705
chemistry of cephalosporin antibiotics. 28. preparation and biological activity of 3-(substituted)vinyl cephalosporins.3-(substituted)vinylcephem nuclei have been prepared by the reaction of 3-formylcephem derivatives with stabilized phosphoranes. appropriate synthetic steps allowed preparation of a series of 3-ethoxycarbonylvinyl- and 3-carboxyvinylcephem derivatives bearing a variety of 7-acylamino functions. the phenoxyacetyl and thiopheneacetyl derivatives of the 3-cyanovinylcephem nucleus were also prepared. although general gram-positive activity was comparable to cephalothin in many cases, against penicil ...1975808607
evaluation of two simple assay methods for detecting antibiotic residues in chicken and pig muscle. 1975806569
absorption of phenethicillin from oral paediatric formulations. 1975806767
investigations on the efficacy of surface disinfection and surface cleaning procedures. 1. tests under real-life conditions.the suitability of disinfection preparations is assessed on the basis of laboratory tests, different methods being used in the various countries. since such model tests are rather inadequate when it comes to judging surface disinfectants, additional in-use tests are desirable. they might, in any case, serve as a reference system for judging the evaluation criteria which still differ widely at the moment. the experiments described in this study were chiefly designed to establish the effect of cle ...19751199525
effect on microorganisms of volatile compounds released from germinating seeds.volatile compounds evolved from germinating seeds of slash pine, bean, cabbage, corn, cucumber, and pea were evaluated for their ability to support growth of microorganisms in liquid mineral salts media lacking a carbon source. growth of eight bacteria was measured turbidimetrically and of six fungi as dry weight of mycelium. volatiles caused increased growth of pseudomonas fluorescens, bacillus cereus, erwinia carotovora, agrobacterium tumefaciens, a. radiobacter, rhizobium japonicum, mucor muc ...19751201509
[electron microscope study of bacteria in dental plaques following the application of fluorides (author's transl)].gels containing fluorides can have profound effects on dental plaques. these gels contain 2% fluorides in the form of sodium monofluorophosphate and sodium fluoride and are buffered to ph 6.5 when applied to teeth they: - encourage the disappearance of certain bacteria, - inhibit the division of cells and diminish their storage of glycogen, - cause a thickening of the bacterial cell wall, - lead to modifications of the bacterial cell membrane, - precipitate the degeneration of most forms of bact ...19751229981
inhibition of microbial growth by fatty amine catalysts from polyurethane foam test tube plugs.when polyurethane foam test tube plugs are autoclaved, they release volatile fatty amines that inhibit the growth of some microorganisms. the chemical structures of these amines were determined by the use of a gas chromatograph-mass spectrometer. they are catalysts used to produce the foam. the problem of contaminating growth media with toxic substances released from polymeric materials is discussed.19751096816
investigations on the efficacy of surface disinfection and surface cleaning procedures. 3. evaluation of the results of in-use and laboratory tests.3 disinfectants and 3 disinfectant cleaning agents were subjected to comparative tests as to their germicidal activity. these were (a) laboratory tests with germ carriers and (b) in-use tests with sarcina-contaminated floor surfaces in a medical institute. the results, details of which are given in the two preceding publications, were assessed under the following angles: validity of the test methods, requisite changes of the test procedures and practical usefulness of the different types of prep ...19751106070
amino sugar and amino acid constituents of the cell walls of the extremely halophilic cocci.cell wall hydrolysates of nine strains of extremely halophilic cocci all contained gylcine, glucosamine, galactosamine, and gulosaminuronic acid. muramic acid was not present in any of the strains.19751119892
microbial degradation of erythromycins a and b.growing cultures, as well as broken and lyophilized cells of pseudomonas 56 were found to degrade erythromycin a, and lyophilized cells inactivated erythromycins a and b. the enzyme system involved in this degradation was constitutive and the enzyme level in the cells could be increased about 8-fold when oleandomycin or erythromycin b was added to the growth medium. the ability of whole or broken cells to inactivate erythromycin a was completely lost when these preparations were boiled, and the ...19751150530
[distribution of rifamycin sv in de bovine tissues following intramammary administration of rifamastene (author's transl)].depending on the physiological activity of the udder, from 4 to 95 per cent of the rifamycin sv administered by intrammary injection are absorbed from the udder and approximately 95 per cent of the absorbed rifamycin sv are excreted through the liver. the rifamycin sv eliminated in the faeces will lose its microbiological activity more or less rapidly, depending on the temperature and oxygen tension. accumulation of rifamycin sv in the meat and organs will not occur on intramammary administratio ...19751154345
inhibitory effects of citrates in the determination of trace amounts of penicillin.in the microbiological assay for the determination of trace amounts of penicillin, it has been shown that sodium citrate and penicillin act synergistically against sarcina lutea. the inhibitory action of citrate appears to involve a sequestering of divalent cations essential to the growth of the organism as shown by minimal inhibitory concentration and agar diffusion studies. the addition of calcium or magnesium to broth or agar at various concentrations overcomes this inhibition, and 0.02 m mag ...19751164006
n-acetyl-l-phenylalanyl-l-phenylalaninol a metabolite of emericellopsis salmosynnemata.a new metabolite n-acetyl-l-phenylalanyl-l-phenylalaninol was isolated from culture filtrates of emericellopsis salmosynnemata which produces zervamicins i and ii. the structure was assigned from spectral properties and degradative studies.19751184465
mechanism of the inhibitory effect of pas granules on the absorption of rifampicin: adsorption of rifampicin by an excipient, bentonite.the bioavailability (plasma concentrations, auc and urinary excretion) of an oral solution of rifampicin was investigated in six healthy volunteers. simultaneous administration of pas granules produced a significant decrease in the absorption of rmp, whereas na-pas tablets had no effect. this indicated that the dosage form of the granules and not pas itself was responsible for the interaction, and that the dissolution of rmp was not involved. the interaction could be reproduced by giving dummy g ...19751233228
behaviour of microbial cells on columns of some types of sephadex gels. 19751104652
investigations on the efficacy of surface disinfection and surface cleaning procedures. 2. laboratory testing of the efficacy under conditions simulating those of real-life.using a standardised method (impression method by means of 'rodac' plates and glass as well as ceramic surfaces as germ carriers) we tested for the efficacy of 3 surface disinfectants (aldehydes, aldehydes + detergent substances, phenol derivatives) and 3 disinfectant cleaning agents (aldehydes + detergent substances + wax) and 2 per cent soft soap solution on staphylococcus aureus, klebsiella pneumoniae and sarcina lutea. the method enabled us to calculate the actual germ count reduction and al ...19751106069
expandable silicone implants for scleral buckling. iii. experiments in vivo.experimental scleral buckling was obtained in rabbits by using expanded silicone rubber implants under scleral trap doors. buckle heights ranged from 2.3 to 4mm after injection of 0.05 to 0.15 ml of fluid into the implants. therate of decrease in buckle height followed up opthalmoscopically for five months, was found to be slow and continous and was caused mainly by loosening by the scleral flaps rather than by the diffusion of fluid from the implants. implants expanded with chorampenicol soluti ...19751147807
the effect of impairment of renal function and dialysis on the serum and urine levels of clindamycin.a single 150 mg oral dose of clindamycin was given to three healthy volunteers and 13 patients with varying degrees of renal functional impairment. the mean peak serum levels in the two groups were 2.55 +/- 0.92 mug/ml and 3.39 +/- 0.68 mug/ml respectively. in all patients the levels greatly exceeded the minimum inhibitory concentration for sensitive pathogens. the serum half-life was extremely variable in patients with renal failure and bore no relationship to the glomerular filtration rate. ...19751057932
[qualitative and quantitative evolution of proteins during the growing curve of sarcina flava]. 19751234618
[inactivation of antifungal agents by cellular components of sarcina lutea]. 19751240276
[generation time of pure cultures of heterotrophic microorganisms isolated from lake baĭkal].generation time was determined in pure cultures of heterotrophic microorganisms in the conditions similar to those of baikal in june--july of 1972. generation time was found to be 37+/-7, 16+/-2.5, 16+/-3.2, and 10+/-2.5 hours, respectively, when the cultures had been diluted with baikal water in the following rations: 1 : 0,1 : 5,1 : 10, and 1 : 20. no differences in the growth rate were found among 11 cultures of heterotrophic microorganisms isolated from baikal. conditions limiting the microb ...19751240574
mechanism of dna degradation induced by neocarzinostatin in bacillus subtilis.when logarithmically growing bacillus subtilis cells were exposed to the antitumor protein, neocarzinostatin (ncs), at a concentration of 50 mug/ml, cellular dna was gradually degraded into an acid-soluble form (up to 60 percent of total dna). the degradation appeared to initiate at the growing regions of dna and to proceed sequentially from the nascent regions to preexistent dna. concomitantly with, or perhaps as a consequence of, the degradation of growing regions, dna detached from the cell m ...1975123897
survey of antibiotic residues in canadian slaughter animals.kidneys and urine of cattle, swine, sheep and chickens were tested for bacterial growth inhibitors using bacillus subtilis and sarcina lutea as test organisms. results were as follows: 211 beef kidneys four positive, 611 swine kidneys five positive, 27 sheep and 120 chicken kidneys all negative, 2108 beef urine 76 positive, 2409 swine urine 186 positive, 176 sheep urine 17 positive. strongest reactions were obtained with b. subtilis on phosphate buffered ph 6.0 plates. larger zones were produced ...1975236082
isolation and structure assignment of an antimicrobial principle from the volatile oil of nigella sativa l. seeds.refrigeration of the volatile oil of nigella sativa l. seeds eventuates in a crystalline substance. the chemical structure of the compound was drawn from its chemical behaviour, as well as from its uv, ir, pmr and mass spectral data. the compound was found to be thymohydroquinone: confirmation of the structure was established via the preparation of its corresponding diacetate ester. the compound was found to have high antimicrobial effect against gram positive microorganisms1975238225
the bacteriological ecosystem of the skin of children in an african tropical envirovment (tanzania).using a standardized method for sampling, the aerobic microbial flora of the skin of ninety-five children in dar es salaam, tanzania, was studied qualitatively and quantitatively. the subjects comprised three groups; group a, thirty-seven normal infants (below the age of 1 year); group b, twenty-nine infants with various bacterial skin lesions; and group c, twenty-nine nursery school children between 4-5 and 7 years. the following observations were made: (i) the quantitative results indicated; ( ...1975239739
[antibacterial activity and presence of mercury in 12 species of marine algae]. 1975820360
[antibacterial activity of a lysozyme-like enzyme from staphylococci].a lysozyme-like enzyme isolated from the culture broth of staph. aureus 712 presented in its native state a lipoproteid complex. the lytic and antibacterial spectrum of the enzyme was similar to that of the egg albumin enzyme. however, the lipoproteid complex was somewhat superior to the egg albumin complex in its activity against micrococcus lysodeikticus, a number of gramnegative bacteria and staphylococci. out of the organisms studied microccus lysodeikticus, sarcina and bac. subtilis proved ...1975813563
comparison of the effects of three fluorocarbons on certain bacteria.three fluorocarbons were tested to determine their effect on bacterial growth. freon 11 and 21 in various concentrations had an inhibitory effect on selected test organisms, but freon 22 had no effect. both aerobic and anaerobic microorganisms, as well as gram-positive and gram-negative species, were included among the bacteria tested. freon 11 and 21 caused a similar response with freon 11 being more inhibitory to some species and freon 21 more inhibitory to others. inhibition was dependent on ...1975803403
singlet excited oxygen as a mediator of the antibacterial action of leukocytes.human polymorphonuclear leukocytes kill a colorless mutant strain of sarcina lutea much more readily than a carotenoid-containing strain. a similar protective effect has been reported in the organism during photodynamic inactivation, where it is attributable to the quenchintg of singlet excited oxygen by carotenoids. the findings with leukocytes support the suggestion that singlet excited oxygen acts as one of the mediators of their bactericidal action.19744415781
the interdependence between high cadmium content of horse kidney cortex and the false positive kidney test for the detection of antibiotic residues at meat inspection. 19744416331
pre-neocarzinostatin, a specific antagonist of neocarzinostatin. 19744281770
carbenicillin pharmacokinetics in rats with reduced renal parenchyma (with special reference to combined use of gentamicin and carbenicillin). 19744426719
microbiological diffusion assay i: operations studied with cooper equation. 19744427274
continuous production of urocanic acid by immobilized achromobacter liquidum cells. 19744441634
studies on the absorption, distribution, metabolism, and excretion of propionylmaridomycin in rats.the absorption, distribution, metabolism, and excretion of propionylmaridomycin was investigated in the rat by using [(14)c]propionylmaridomycin. propionylmaridomycin was absorbed from the gastrointestinal tract at a relatively rapid rate and was readily distributed into tissues. among the tissues examined, liver, kidney, and lung showed a remarkably higher level of the radioactivity than plasma, while distribution of the radioactivity into brain was little. however, a significant accumulation o ...19744451342
in vitro bioassay method for antiamoebin. 19744461714
microbiological assay of oleandomycin in feed: collaborative study. 19744472976
studies in humic acid-decomposing bacteria. iv. the ability of some bacteria to decompose aromatic compounds. 19744480501
chemical composition of a purified membrane fraction from sarcina aurantiaca in relation to growth phase. 19744545202
variation in the lipid and fatty acid composition in purified membrane fractions from sarcina aurantiaca in relation to growth phase. 19744545203
effects of concentration of volatile metabolites from bacteria and germinating seeds on fungi in the presence of selective absorbents. 19744207172
[studies on the stability of antitumor protein, neocarzinostatin. i. stability of solution of neocarzinostatin (author's transl)]. 19744282494
[studies on the stability of antitumor protein, neocarzinostatin. ii. stability of injection of neocarzinostatin (author's transl)]. 19744282495
relative biological activities of individual streptovaricins and streptovaricin acetates. 19744130531
studies on the biological activities of actinomycins z1 and z5. 19744133977
[the antibacterial and chemotherapeutic activity of the soviet produced antibiotic rifamitsina sv]. 19744156904
phagocytic activity in the retinal pigment epithelium of the frog rana pipiens. ii. exclusion of sarcina subflava. 19744363810
effect of viscosity on bacterial motility.the behavior of a number of motile flagellated bacteria toward viscosity characteristics of their fluid environments was observed. all showed an increase in velocity (micrometers per second) in more viscous solutions. velocity reached a maximum at a characteristic value, however, and thereafter decreased with higher viscosities. peritrichously flagellated bacteria had maximum velocities at higher viscosities than polarly flagellated bacteria. effects of temperature, and possible utilization of c ...19744204439
effectiveness of footwear decontamination methods for preventing the spread of infectious agents. 19744374591
stomach fermentation in east african colobus monkeys in their natural state.the microbial fermentation in the stomachs of two monkeys, colobus polykomos, collected in kenya, was studied. the gas accumulated within the stomach contained h(2) but no ch(4). volatile fatty acid concentrations were high, but accumulated acid prevented determination of the fermentation rate in untreated, incubated stomach contents. upon addition of bicarbonate, a very rapid rate could be demonstrated. some d- and l-lactate were in the stomach contents. starchy seeds or fruits rather than leav ...19744207763
studies on the activated sludge bacteria participating in the biodegradation of methanol, formaldehyde and ethylene glycol. i. isolation and identification. 19744209888
studies on the activated sludge bacteria participating in the biodegradation of methanol, formaldehyde and ethylene glycol. ii. utilization of various carbon and nitrogen compounds. 19744209889
[dissolution of gold by microorganisms and the products of their metabolism]. 19744210177
rifampicin in plasma and pleural fluid after single oral doses. 19744212324
studies on the bacteriolytic activity of streptomyces albus culture filtrates. 1. the effect of variations in cultivation conditions and the screening of various enzyme specificities. 19744212365
studies on the bacteriolytic activity of streptomyces albus culture filtrates. 2. fractionation on ion-exchange chromatography and gel filtration. 19744212366
studies on the bacteriolytic activity of streptomyces albus culture filtrates. 3. affinity for chitin. 19744212367
glycoside cleavage reactions on erythromycin a. preparation of erythronolide a. 19744213128
phoratoxin, a toxic protein from the mistletoe phoradendron tomentosum subsp. macrophyllum (loranthaceae). immunological properties and tests for proteinase inhibiting and antibiotic effects. 19744213295
synthesis of a 5-nitrofuran derivative of expected antimicrobial action. 19744213930
the relationship between platelets and bacteria. 19744214297
absorption and decomposition of potassium-35s-phenoxymethyl penicillin. 19744214641
synthesis and antibacterial activity of 5-nitro-2-furfurylidene p-sulfonyl- and p-sulfamoylbenzoylhydrazides. vii. 19744214919
synthesis of 4'-deoxykanamycin and its resistance to kanamycin phosphotransferase ii. 19744215783
plaque formation in bacillus subtilis, and growth inhibition of bacillus subtilis and sarcina sp., induced by schizophyllum commune. 19744216400
[dynamics of decomposition of labeled algae by bacteria]. 19744216746
[taxonomy of methanosarcina]. 19744217880
[effect of dynamic factors of space flight on the survival of microorganisms and their iron-porphyrin enzymes]. 19744217881
bacterial density in operation wounds. 19744218421
violamycin, a new red-pigment antibiotic. 19744219245
[studies on the specificity of antisera against denaturated desoxyribonucleic acids]. 19744219522
microbiological assay and tissue distribution of mithramycin (nsc-24559) in mice. 19744276405
selenium heterocycles. x. synthesis and antibacterial activity of pyridyl-1,2,3-thiadiazoles and pyridyl-1,2,3-selenadiazoles. 19744208197
total synthesis of bisnorpenicillin v. 19744208535
[actinomycin d-o]. 19744142853
[antibacterial effect of rifampicin and its chemotherapeutic properties in experiments on animals]. 19744155605
studies on a bactericidal substance produced by group a streptococci. 19744599409
enrichment and selective isolation of sarcina maxima lindner. 19744599711
mechanisms of corrin dependent enzymatic reactions. 19744609867
partition of proteins and micro-organisms in aqueous biphasic systems. 19744610354
microbial flora of nurses' hands. ii. qualitative differences in occupational groups. 19744611443
carotenoid chromophore length and protection against photosensitization. 19744362529
[effect of satellite microorganisms on the growth of leptospira colonies]. 19744369548
[contamination in the dental office and possibilities of a planned decontamination under the aspect of rationalization of the practice]. 19744597838
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