Publications

[pathogenicity of staphylococcus asaccharolyticus distaso].		1954	13171675
glutaconic acid, a product of the fermentation of glutamic acid by peptococcus aerogenes.		1966	5963335
the effect of sodium on the fermentation of glutamic acid by peptococcus aerogenes.		1967	6033744
metabolism of histidine by peptococcus aerogenes.		1967	6035532
the isolation and fractionation of plasmodium knowlesi.		1969	4987063
the production of alpha-hydroxyglutaric acid from glutamic acid by cell-free preparations of peptococcus aerogenes.		1969	4312225
[reproduction of experimental hepatitis in puppies].		1970	4316401
studies on the structure of the mitochondrial membranes.		1970	4245917
action of an n-acetylmuramidase from a limax amoeba on the murein of proteus mirabilis.		1971	4256271
[comparative study of the convulsion provoking activity of actinomycin d and methionine sulfoximine injected into the cerebrospinal fluid of dogs].		1971	4262072
flow and composition of lymph collected from the skeletal muscle of the rabbit hind limb.		1972	5085348
modification by orthophosphate of the transfer of tooth phosphorus into cariogenic streptococci.		1972	4500617
alpha-ketoglutarate as an intermediate in glutamate metabolism by peptococcus aerogenes.		1972	4338317
purification and characterization of glutamic acid dehydrogenase and -ketoglutaric acid reductase from peptococcus aerogenes.		1972	4338318
bovine abortion associated with candida tropicalis.		1973	4692299
[chromosomal phylogeny of man and hominian primates (attempt at reconstitution of the karyotype of the the common ancester)].		1973	4738485
[epoch-making reorganization in home nursing: viborg's demand for home nursing applies to the entire day].		1974	246608
fluorescent antibody study of the gram-positive anaerobic cocci.	fluorescent antibody conjugates were prepared from five species of anaerobic cocci commonly isolated from human infections. when tested with homologous and heterologous cells these conjugates were found to be highly specific. there was no evidence of a common genus antigen. peptococcus magnus conjugates detected a species-specific antigen; cross-reactions with peptostreptococcus anaerobius were readily eliminated by absorption. the conjugates from peptococcus asaccharolyticus, peptococcus prevot ...	1974	4140708
erwinia rhapontici (millard) burkholder associated with pink grain of wheat.		1974	4608652
two pathways of glutamate fermentation by anaerobic bacteria.	two pathways are involved in the fermentation of glutamate to acetate, butyrate, carbon dioxide, and ammonia-the methylaspartate and the hydroxyglutarate pathways which are used by clostridium tetanomorphum and peptococcus aerogenes, respectively. although these pathways give rise to the same products, they are easily distinguished by different labeling patterns of the butyrate when [4-(14)c]glutamate is used as substrate. schmidt degradation of the radioactive butyrate from c. tetanomorphum yie ...	1974	4813895
comparison of oxidation-reduction site geometries in oxidized and reduced chromatium high potential iron protein and oxidized peptococcus aerogenes ferredoxin.		1974	4417854
preparative isoelectric focusing of human serum high-density lipoprotein (hdl3).		1974	4373056
the influence of age on the wound healing of experimental myocardial infarction in rats.	in 6-weeks-old, 5-months-old and 21-months-old rats myocardial infarction was induced by coronary artery ligature. after performing the ligature the animals were administered 3h-thymidine, 3h-proline or 35s-sulphate at different times. the following parameters were determined: number of dna- and tropocollagen-synthesizing connective tissue cells at the infarction border and at the infarction site; mean silver grain density above the nuclei or cells; duration of a cycle; number of mitoses, and in ...	1975	134906
calcium uptake and atpase activity of microsomes from heterotopically transplanted rabbit heart.	heterotopic cardiac transplantation was performed in rabbits. the calcium uptake and atpase activities of microsomal fraction were studied in three groups (group i, normal controls; group ii, early transplants; group iii, late transplants) during severe rejection as characterized histologically and electrocardiographically. group iii showed a significant decrease in calcium uptake but no difference in atpase activity compared with groups i or ii. the results demonstrate that in the rejecting tra ...	1975	127353
the possible role of tightly bound adenine nucleotides in oxidative and photosynthetic phosphorylation.	the tightly bound nucleotides of the beff-heart mitochondrial atpase are released during cold inactivation followed by ammonium sulfate precipitation. during incubation at 0 degrees c the sedimentation coefficient (s20w) of the atpase first declines from 12.1s to 9s. prolonged incubation or precipitation with ammonium sulfate leads to dissociation of the 9s component into subunits with s20w of 3.5s. the 9s component still bears bound nucleotides which exchange more extensively and rapidly with a ...	1975	127089
letter: light induced change in rod outer segment membrane fluidity.		1975	165628
steroid production by quartered and capsular rat adrenal gland in response to haemorrhage.	60 min after rapid bleeding (1.5--2.0 per cent of b. w.) both aldosterone and corticosterone production rate by quartered rat adrenals were found to be elevated. however, no difference was observed in the rate of aldosterone and corticosterone production by capsular adrenals of sham operated and hypovolaemic rats. corticosterone production rate by decapsulated adrenals was much more higher after haemorrhage than in the control group. the same alterations could be observed incubating adrenal tiss ...	1975	179800
the class i (schiff base) fructose-diphosphate aldolase of peptococcus aerogenes.		1975	237182
[the serological behaviour of the pyogenic anaerobes, peptococcus asaccharolyticus and p. prevotii (author's transl)].	using rabbit antisera against whole-cell adjuvant-free antigens, the serological behaviour of ten butyrate-producing peptococcus strains, five belonging to p. asaccharolyticus and five to p. prevotii, was studied in cross-agglutination and gel-diffusion experiments. agglutination occurred in all the homologous and nearly one third of the heterologous antigen-antibody systems. cross-reactions, however, were clearly restricted to members of the same peptococcus species.	1975	818602
nh---s hydrogen bonds in peptococcus aerogenes ferredoxin, clostridium pasteurianum rubredoxin, and chromatium high potential iron protein.	results from refinement of the crystal structures of p. aerogenes ferredoxin and c. pasteurianum rubredoxin determined by x-ray diffraction show that there are 15-18 nh---s bonds in the former and six in the latter with lengths in the range 3.1-3.9 a. earlier tritium exchange experiments are consistent with the presence of these hydrogen bonds in the ferredoxin structure and show that more peptide hydrogen atoms are available for exchange in apoferredoxin than in intact ferredoxin. four types of ...	1975	1061073
comparative susceptibility of anaerobic bacteria to minocycline, doxycycline, and tetracycline.	the comparative susceptibility of 622 recent clinical isolates of anaerobic bacteria to minocycline, doxycycline, and tetracycline was determined by an agar-dilution technique. in addition to bacteroides fragilis, a variety of other anaerobic bacteria was resistant to achievable blood concentrations of tetracycline (55% inhibited by 6.25 mug/ml) and doxycycline (58% inhibited by 2.5 mug/ml). in contrast, minocycline was significantly more active (p < 0.05) than both doxycycline and tetracycline, ...	1975	1137358
determination of the iron-sulfur distances in rubredoxin by x-ray absorption spectroscopy.	the high intensity x-ray flux from the synchrotron radiation at the stanford synchroton radiation project has been used to study the extended x-ray absorption fine structure (exafs) of the iron-sulfur protein peptococcus aerogenes rubredoxin. absorption measurements were made from 7080 ev, which is below the k-edge of iron, to about 650 ev above the edge and structure was obtained over the entire region. by means of a model iron-sulfur compound for evaluating the phase shifts, the variation of t ...	1975	1060082
structure of peptococcus aerogenes ferredoxin. refinement at 2 a resolution.	the model for ferredoxin from peptococcus aerogenes, derived from a 2.8-a resolution map, has been refined with a 2-a resolution data set. the conventional index, r, decreased from 0.449 for the initial model to 0.188 for the unconstrained one and 0.206 for the constrained one. the standard deviations of the iron and sulfur atoms are 0.04 to 0.05 a and for the oxygen, nitrogen, and carbon atoms they range from 0.14 to 0.26 a. one hundred forty-six water oxygen atoms were included in the solvent ...	1976	932007
lactic acid-utilizing bacteria in ruminal fluid of a steer adapted from hay feeding to a high-grain ration.	a mature, rumen-cannulated steer fed coastal bermuda grass hay for 8 weeks was adapted to a high-grain ration by stepwise increases in grain over a period of 4 weeks. the grain rations had concentrate-to-roughage ratios of 40:60, 70:30, and 85:15 and were fed for 10, 7, and 11 days, respectively. numerical estimates of lactate-utilizing bacteria in ruminal fluid of the steer the last 3 days each ration was fed were made by colony counts. lactate-utilizing bacteria were identified as megasphaera ...	1976	1275348
long-chain fatty acids of peptococci and peptostreptococci.	the long-chain fatty acids extracted from the whole cells of 12 clinically significant species of peptococci and peptostreptococci were characterized by gas-liquid chromatography. the resulting methylated fatty acid profiles (and some unidentified compounds) of 82 strains allowed the 12 species to be separated into four groups. fifteen strains of peptostreptococcus anaerobius were placed in group i because they had a unique, prominent compound that occurred in the area where a c8 to c10 fatty ac ...	1976	1002830
effectiveness of orally administered bordetella pertussis vaccine in mice.	oral administration of killed bordetella pertussis organisms to mice results in increased resistance to an intracerebral infection with virulent b. pertussis cells. the rate of survival is dependent on the dose of antigen. but besides specific systemic immunity, which is persistent over a long period, also transient non-specific resistance is increased. these effects are evidently induced without penetration of bacterial substances into the circulation.	1976	182602
oxidation-reduction properties of several low potential iron-sulfur proteins and of methylviologen.	apparent oxidation-reduction potentials at ph 7.0 and 25 degrees c were determined using the h2-hydrogenase system with ferredoxins from the following sources: clostridium pasteurianum, -403 mv; c tartarovorum, -424 mv; c. acidi-urici, -434 mv; peptococcus aerogenes, -427 mv; chromatium d, -482 mv (ph 8.0); b. polymyxa, fd i, -377 mv, and fd ii, -422 mv; and spinach, -428 mv. the ph dependence of these values was variable, ranging from -2 to -24 mv/ph unit increase for different ferredoxins. ove ...	1976	181047
direct assignment of the cysteinyl, the slowly exchangeable, and the aromatic ring 1h nuclear magnetic resonances in clostridial-type ferredoxins.	we have directly assigned the 1h nmr corresponding to the cysteinyl protons, the slowly exchangeable protons, and the aromatic ring protons in the 1h nmr spectrum of clostridium acidi-urici ferredoxin by isotopic labeling and 13c nmr decoupling techniques. we also show that the resonance pattern in the 8- to 20-ppm (from 2,2-dimethyl-2-sialapentanesulfonic acid) region of the 1h nmr spectra of oxidized clostridium acidi-urici, clostridium pasteurianum, clostridium perfringens, and peptococcus ae ...	1977	191457
inherited metabolic disease: prospects for the future in both basic and clinical research.	the birth of a child with an inherited disorder is often the beginning of a life-long problem for the whole family. about 8.5% of paediatric deaths and 4.7% of paediatric hospital admissions are due to autosomal and sex-linked recessive diseases. these figures are likely to be erroneously low because of incomplete ascertainment. the inherited metabolic diseases therefore merit study on economic as well as humanitarian grounds. investigations of the disorders of purine metabolism have been conduc ...	1977	272981
[influence of horizontal immersion on urinary excretion of catecholamines].	normal man submitted to thermoneutral water immersion in horizontal position presents an increase in diuresis, natriuresis and creatininuria. noradrenalinuria and adrenalinuria are reduced, indicating a decrease in orthosympathetic activity.	1977	148963
the germicidal effect of the open air in different parts of the netherlands.	using the microthread technique the survival of escherichia coli mre 162 in open air was measured in different parts of the netherlands. the presence of bactericidal compounds (open air factor = oaf) could be demonstrated on several days and quantitated in relative units of oaf concentration. in the absence of ozone the oaf concentration was always low. in the presence of ozone the oaf concentration was dependent on wind direction. at the selected microthread exposure sites air from areas with h ...	1977	321676
effect of ruminal lactic acid-utilizing bacteria on adaptation of cattle to high-energy rations.	heifers, unadapted to a concentrate ration, were intraruminally inoculated (1 dose) with cultures of ruminal lactic acid-utilizing bacteria or with ruminal fluid from a steer adapted to a concentrate ration. inoculation with cultures (1 l) of selenomonas ruminantium or megasphaera elsdenii did not produce better average daily weight gains or feed efficiency of heifers fed a high-energy ration for 21 days, if these values were compared with the performance of noninoculated heifers. average daily ...	1977	883707
assignment of the cysteinyl 13c nuclear magnetic resonances and comparison of other aliphatic amino acid resonances of clostridium acidi-urici, clostridium pasteurianum, and peptococcus aerogenes ferredoxins.	13c nmr spectra of clostridium acidi-urici, clostridium pasteurianum, and peptococcus aerogenes ferredoxins show that some 13c resonances of the aliphatic amino acid residues are shifted significantly from their corresponding resonance positions in the spectra of model polypeptides or apoferredoxin. thirteen 13c resonances are shifted into the 80- to 120-ppm (from cs2) region, and have been assigned to the cysteinyl alpha and beta carbon atoms. the remaining shifted resonances in the 120- to 190 ...	1978	701285
studies on the mechanism of formyltetrahydrofolate synthetase. the peptococcus aerogenes enzyme.	two conflicting mechanisms have been proposed for formyltetrahydrofolate synthetase (ec 6.3.4.3). detailed studies with a clostridial enzyme support a sequential mechanism, while a stepwise mechanism with formation of a dissociable intermediate has been proposed for the peptococcus aerogenes synthetase. however, the data supporting the p. aerogenes mechanism were obtained using synthetase of questionable purity and the results supporting the mechanism could be attributed to contaminating activit ...	1978	624720
effect of dexamethasone on electrolyte transport in the large intestine of the rat.		1978	149689
an unstable donor-recipient dna complex in transformation of bacillus subtilis.	in re-extracted dna obtained shortly after uptake of transforming dna by bacillus subtilis, increased amounts of donor dna radioactivity banding at the position of donor-recipient dna complex (drc) are observed in cscl gradients, if the cells are irradiated with high doses of uv prior to reextraction of the dna. qualitatively, the same phenomenon is observed if lysates of transforming cells are irradiated. uv-irradiation of lysates of competent cells to which single-stranded dna is added after l ...	1978	106232
aerobic and anaerobic cervical flora of healthy thai women.	the bacteriologic study of the cervical flora in 50 healthy women revealed that 70% harbored mixed aerobic and anaerobic organisms. aerobic organisms alone were recovered in 15 women (30%). polymicrobial organisms were found in all but five women (in these only one aerobe was isolated per patient). the majority of patients had 1-2 anaerobes, with more than two aerobes. the common aerobes were alpha-hemolytic streptococci, staphylococcus epidermidis and lactobacilli. the most common anaerobes wer ...	1978	39833
occupational chronic exposure to organic solvents. vii. metabolism of toluene in man.		1979	429070
a nuclear mutation conferring aurovertin resistance to yeast mitochondrial adenosine triphosphatase.	a single gene nuclear yeast mutant was isolated whose mitochondrial f1-atpase was resistant to the specific f1 inhibitor aurovertin. the mutant enzyme was not cross-resistant to other f1 inhibitors. the binding of aurovertin to f1 and to the two largest f1 subunits (alpha and beta) was measured by enhancement of aurovertin fluorescence. aurovertin bound to wild type f1-atpase and to its monomeric beta subunit with about the same binding constant. it failed to bind to wild type alpha subunit or t ...	1979	153911
determination of cooperative interaction between clusters in clostridium pasteurianum 2 (4fe-4s) ferredoxin.	the effect of reducing one 4fe-4s cluster in clostridium pasteurianum 2 (4fe-4s) ferredoxin on the reduction potential of the unreduced cluster has been investigated. while such an effect is suggested by both the x-ray structure of peptococcus aerogenes 2 (4f-4s) ferredoxin and the polypeptide conformational change on reduction present in clostridial-type 2 (4fe-4s) ferredoxins, present studies indicate that cluster-cluster cooperative interaction is not strong enough to be of functional importa ...	1979	220247
quantitation of immunoglobulins in calf serum.		1979	433126
biochemical, enzymatic, and serological differentiation of peptococcus indolicus (christiansen) sørensen from peptococcus asaccharolyticus (distaso) douglas.	peptococcus indolicus (formerly micrococcus indolicus) is an asaccharolytic anaerobic coccus that is frequently isolated from udder secretions from cases of heifer and dry-cow mastitis (summer mastitis). to facilitate better identification and its differentiation from peptococcus asaccharolyticus, a variety of biochemical, enzymatic, and serological properties were studied. seventy-nine strains of p. indolicus of bovine origin and 10 strains of p. asaccharolyticus of human origin were examined u ...	1979	372218
preparative isolation of the cell receptor for immunoglobulin e.		1979	89114
urease-producing species of intestinal anaerobes and their activities.	urease activities of anaerobic bacteria that constituted predominant gut flora were examined. it was demonstrated that some strains of eubacterium aerofaciens, e. lentum, and peptostreptococcus products produced urease. they were the most numerous species in human feces. all strains of bifidobacterium infantis and some strains of bacteroides multiacidus, b. bifidum, clostridium symbiosum, fusobacterium necrophorum, f. varium, lactobacillus fermentum, peptococcus asaccharolyticus, and p. prevotii ...	1979	36839
polyribonucleotide inhibition of ribonucleic acid directed deoxyribonucleic acid polymerase of mouse mammary tumor (type b) virus and simian sarcoma (type c) virus.		1979	90331
the elephant man.		1979	90342
effect of ethylenediaminetetraacetate on phospholipids and outer membrane function in escherichia coli.	treatment of escherichia coli k-12 strain s15, containing a normal amount of phospholipase a, with ethylenediaminetetraacetate (edta) resulted in an increase in sensitivity of the organism to actinomycin d. strain s17, a mutant deficient in both detergent-resistant phospholipase a and detergent-sensitive phospholipase a, was considerably less sensitive to the antibiotic after the treatment. both strains released lipopolysaccharide after edta treatment, indicating that this outer membrane compone ...	1979	104974
multiple sclerosis: immunochemical studies on the demyelinating serum factor.	sera from patients with multiple sclerosis (ms) frequently produce demyelination of central nervous system tissue cultures. the nature of the factors responsible for demyelination is not as yet clearly established. however, several authors previously reported, in in vivo and in vitro models, demyelinating activity in igg fractions isolated from sera and cerebrospinal fluid of ms patients14,42,48. we found the demyelinating activity of ms sera to be extremely labile to conventional biochemical tr ...	1979	105780
differentiation of peptococcus and peptostreptococcus by gas-liquid chromatography of cellular fatty acids and metabolic products.	gas-liquid chromatographic (glc) profiles of cellular fatty acids and metabolic products were useful in identifying strains of peptococcus saccharolyticus, peptococcus asaccharolyticus, peptostreptococcus anaerobius, peptostreptococcus micros, and streptococcus intermedius. the glc results supported the recent taxonomic decision to transfer aerotolerant peptostreptococcus species to the genus streptococcus. because inconsistencies in the results prevented our differentiating peptococcus prevotii ...	1979	528680
anaerobic bacteria in late infections following orthopedic surgery.	twelve patients with late anaerobic infection following hip or knee arthroplasty or tibia osteosynthesis are presented. peptococci (peptococcus asaccharolyticus, p. magnus, p. prevotii) and peptostreptococci (peptostreptococcus micros, p. anaerobius, p. parvulus) were present in nine of the cases. anaerobic gram-positive or -negative rods were found in three cases (propionibacterium acnes, actinomyces israelii, and bacteroides fragilis). antibodies against the isolated bacteria were detected in ...	1979	492071
survival of anaerobic bacteria in common laboratory diluents.	the survival of six species of anaerobic bacteria was studied in simple or commercially available diluents. bacteroides fragilis and fusobacterium nucleatum showed excellent survival in all diluents including distilled water. fusobacterium mortiferum survived well in all diluents except water and water supplemented with 0.1% gelatain. clostridium perfringens survived best in phosphate-buffered saline with gelatin. peptococcus asaccharolyticus required gelatin added to the basic diluent, and stre ...	1979	479359
treatment of the incompletely formed tooth.		1979	275360
teaching the teachers.		1979	243225
antigenic relationships and rapid identification of peptostreptococcus species.	antisera against whole cells of each peptostreptococcus species (p. anaerobius, p. micros, p. parvulus, and p. productus) were produced in rabbits. when these antisera were reacted against sonically disrupted cells and culture supernatant fluids in ouchterlony tests, lines of identity were obtained among the antigens from all the species and uninoculated culture medium. when the antisera were subsequently absorbed with the dehydrated culture medium used to grow the peptostreptococci, all cross-r ...	1980	6769956
urinary tract infection caused by anaerobic bacteria in children.	anaerobic bacteria were recovered from 5 children with urinary tract infection (uti). three had pyelonephritis and 2 cystitis. two of the patients had a history of prior recurrent uti. urine samples were collected using suprapubic aspiration. the anaerobic organism recovered were 3 isolates of bacteroides fragilis and one each of b. melaninogenicus, peptococcus asaccharolyticus, and bifidobacterium adolescentis. mixed infection was present in 3 children. in 2 cases b. fragilis were present with ...	1980	7445306
peptococcla urinary tract infection with bacteremia: a case report.	the second documented case of urinary tract infection with associated bacteremia of peptococcus asaccharolyticus origin is reported. high fever and low peripheral white blood count were manifestations of bacteremia with pepococcus asaccharolyticus, an anaerobic gram-positive coccus. although anaerobes have been documented to cause urinary tract infections resulting in bacteremia most previous reports implicate aerobes in these processes. the problems inherent in culturing urine for anaerobes are ...	1980	7411710
effect of temperature, humidity and exposure to oxygen on the survival of anaerobic bacteria.	the relative effect of humidity, temperature and exposure to oxygen on the survival of 11 strains of bacteriodes spp., fusobacterium necrophorum, eubacterium lentum, clostridium ramosum, peptococcus asaccharolyticus and peptostreptococcus anaerobius was evaluated. suspensions of the test strains were applied to membrane filters either in empty sterile petri dishes or on plates of non-nutrient agar to provide dry or moist conditions. the filters were stored aerobically or anaerobically in an atmo ...	1980	7431377
aerobic and anaerobic bacterial flora of burns in children.	aerobic and anaerobic bacterial flora of burn sites in 180 children were monitored. specimens were obtained twice a week; each patient had between one and 21 cultures taken (mean 2.4). a total of 392 specimens were collected over 2 years, of which 319 were positive. aerobic bacteria alone were present in 225 specimens (71%) and anaerobic bacteria alone were present in 26 (8%). mixed aerobic and anaerobic bacteria were present in 68 burn specimens (21%). a total of 580 isolates (448 aerobes and 1 ...	1981	7218398
the vaginal microbial flora in non-specific vaginitis.	the facultative and strictly anaerobic vaginal microbial flora was investigated in 40 women with non-specific vaginitis and in 40 control women seen in private gynaecological practice. gardnerella vaginalis, anaerobic gram-negative bacilli, anaerobic gram-negative and gram-positive cocci were all associated with non-specific vaginitis (p less than 0.001), whereas lactobacilli occurred less frequently in non-specific vaginitis than in controls (p less than 0.01). the most common anaerobes were ve ...	1982	6985215
peptococcus asaccharolyticus bacteremia with liver involvement cured with oral metronidazole.		1982	7117797
purification, characterisation and reconstitution of glutaconyl-coa decarboxylase, a biotin-dependent sodium pump from anaerobic bacteria.	glutaconyl-coa decarboxylase from acidaminococcus fermentans is a biotin enzyme, which is integrated into membranes. it is activated by triton x-100 and inhibited by avidin. the results obtained by a combination of both agents indicate that biotin and the substrate-binding site are located on the same side of the membrane. the decarboxylase was solubilized with triton x-100 and purified by affinity chromatography on monomeric avidin-sepharose. the enzyme is composed of three types of polypeptide ...	1983	6628393
a kinetic study of the oxidative deamination of l-glutamate by peptostreptococcus asaccharolyticus glutamate dehydrogenase using a variety of coenzymes.	the nad+-specific glutamate dehydrogenase from peptostreptococcus asaccharolyticus follows michaelis-menten kinetics in contrast to the enzyme from several other sources, and thus gives linear double-reciprocal plots of initial-rate data. the initial-rate parameters have been determined for this bacterial dehyrogenase in the direction of oxidative deamination. the use of alternative coenzymes leads to some conclusions about the order of substrate addition. an investigation of the ph dependence o ...	1984	6148240
characterization of peptostreptococcus asaccharolyticus glutamate dehydrogenase purified by dye-ligand chromatography.	glutamate dehydrogenase (l-glutamate:nad+ oxidoreductase (deaminating); ec 1.4.1.2) has been purified from peptostreptococcus asaccharolyticus in a single step using dye-ligand chromatography. the enzyme (gdh) was present in high yields and was stabilized in crude extracts. a subunit molecular weight of 49000 +/- 500 was determined by sds polyacrylamide gel electrophoresis and six bands were obtained after cross-linking the subunits with dimethyl suberimidate. this bacterial gdh was predominantl ...	1984	6502134
n-nitrosamine formation by intestinal bacteria.	n-nitrosamine formation by various intestinal bacteria was investigated. n-nitroso-dimethylamine (ndma) formation by viable resting cells of escherichia coli a10 was proportional to the incubation time and the enzyme concentration, while boiled cells were incapable of nitrosation. the enzyme was optimal at ph 7.5 and showed about the same specificities for dimethylamine, diethylamine, dibutylamine, di-isobutylamine, piperidine and pyrrolidine, but high specificity for morpholine. the intestinal ...	1984	6398295
regulation by interleukin 2 of interleukin 2 receptors and gamma-interferon synthesis by human thymocytes: augmentation of interleukin 2 receptors by interleukin 2.	the role of interleukin 2 (il 2) on the expression of il 2 receptors and on the synthesis of gamma-interferon (gamma-ifn) by human thymocytes was investigated. human thymocytes isolated from specimens obtained during cardiac surgery of infants and children were induced with one or all of the following agents: il 2, concanavalin a (con a), and 12-o-tetradecanoylphorbol 13-acetate (tpa). the expression of il 2 receptors and gamma-ifn titers were determined. the results indicate that thymocytes cul ...	1985	2981271
inhibition and activation of bovine heart nad-specific isocitrate dehydrogenase by atp.	in the absence of added calcium, inhibition of nad-specific isocitrate dehydrogenase by atp occurred without adp (i0.5 = 1.8 mm) and with 0.2 mm adp3- (i0.5 = 1.0 mm) at subsaturating substrate concentrations at ph 7.4. inhibition by atp was competitive with nad+ in the presence and absence of adp and was not reversed by magnesium citrate. no reversal of atp inhibition by free ca2+ was observed in the presence of adp (0.2 mm). however, when adp was absent, increasing ca2+ first caused progressiv ...	1985	2990340
chromium: a review of environmental and occupational toxicology.	the following topics are covered in this brief review on the environmental and occupational toxicology of chromium: occurrence, production and uses of chromium and chromium compounds; experimental toxicology; chromium toxicity for man; hygienic and ecologic aspects of chromium contamination of the environment. the review provides a conclusive evidence which suggests that chromium, especially its hexavalent form, is both toxic and carcinogenic, but its trivalent form is physiologically essential ...	1985	3157743
a sodium ion gradient as energy source for peptostreptococcus asaccharolyticus.	the determination of enzymatic activities in cell-free extracts of acidaminococcus fermentans and peptostreptococcus asaccharolyticus led to a refined scheme for the pathway of glutamate fermentation via (r)-2-hydroxyglutarate to acetate and butyrate. from the ratio of these products the amount of atp generated by substrate level phosphorylation was calculated. growth experiments with the organisms including clostridium symbiosum and clostridium tetanomorphum indicated that a sodium gradient con ...	1985	4037980
[a comparative study of the various organisms causing salpingitis and their potential presence in iud wearers].	a prospective study was undertaken to compare the enbdometrial and vaginal bacteria of a continuous series of 283 patients who had iuds removed between june 1, 1979 and june 1, 1983, by the same clinician. measures were taken to avoid contamination of the iud upon removal by the vaginal bacteria, but as a practical matter it was impossible to avoid all contact. the iuds involved were multiload copper 250 or 375, gyne t and t 200, nova t, and dimelys. the number of positive cultures from the ...	1985	12339972
anaerobic bacteria in urine before and after prostatic massage of infertile men.	in the present study 25 infertile men delivered urine before and after prostatic massage. expressed prostatic secretion (eps) was obtained from 11 men. aerobic and anaerobic bacterial analyses showed that a total of 33 isolates was found in samples of urine voided before massage as compared to 59 isolates after massage of the prostate. there was an increase in the number of anaerobes whereas there was no change in the number of aerobes. the occurrence of eps did not influence the number of aerob ...	1985	4000063
evaluation of broth microdilution susceptibility results for anaerobic organisms by use of a rapid direct colony inoculum.	a direct colony inoculum suspension procedure was compared with the overnight suspension procedure recommended for the broth microdilution anaerobic commercial system (micro-media systems, inc., potomac, md.). six national committee for clinical laboratory standards-recommended quality control organisms, bacteroides fragilis atcc 25285, clostridium perfringens atcc 13124, bacteroides thetaiotaomicron atcc 29741, bacteroides vulgatus atcc 29327, peptococcus magnus atcc 29328, peptococcus asacchar ...	1985	3972999
moxalactam versus clindamycin plus tobramycin in the treatment of obstetric and gynecologic infections.	the clinical efficacy of moxalactam versus clindamycin/tobramycin was evaluated in a comparative, randomized, prospective study. sixty patients were treated: 30 with moxalactam and 30 with clindamycin/tobramycin. there were 15 cases of tuboovarian abscess, 36 cases of severe pelvic inflammatory disease with peritonitis, eight cases of endomyometritis, and one wound abscess. aerobic and anaerobic cultures from the sites of infection yielded 441 microorganisms from 53 patients; an average of 8.3 b ...	1985	3895947
amino acid sequence of the [4fe-4s] ferredoxin isolated from desulfovibrio desulfuricans norway.	the complete amino acid sequence of the [4fe-4s] ferredoxin from desulfovibrio desulfuricans norway was determined by repetitive edman degradation of the whole protein and peptides derived from tryptic digestion. the protein has 59 residues. four of the six cysteine residues are involved in the binding of the [4fe-4s] cluster in the same arrangement as in clostridial ferredoxins. this sequence is compared to various desulfovibrio ferredoxin sequences and to the sequence and three-dimensional str ...	1985	4008492
toxicity of perfluorinated fatty acids for human and murine b cell lines.	the toxicity of two perfluorinated fatty acids, penta decafluoro-n-octanoic acid (pfoa) and nonadecafluoro-n-decanoic acid (ndfda), on three mammalian b cell lines was evaluated. cells were exposed to the perfluorinated molecules for either 24 or 48 hr under a variety of culture conditions. immunoglobulin secretion and surface membrane expression were unaffected by both pfoa and ndfda at sublethal concentrations. lethal effects of pfoa and ndfda are diminished by either lowering culture temperat ...	1986	3764929
analysis of edta-soluble cell surface components of gram-positive anaerobic cocci.	the protein content of edta extracts from 76 strains of gram-positive anaerobic cocci was examined using sds-page. strains of peptostreptococcus anaerobius produced almost identical profiles; greater heterogeneity was observed within the species peptococcus magnus, peptococcus prevotii and peptococcus asaccharolyticus, but several strains within each biotype produced similar patterns. serological investigation of these extracts by elisa revealed numerous cross-reactions among the different bioty ...	1986	3806050
[polarographic method for determining activity of dehydrogenases of the pentosephosphate pathway of carbohydrate metabolism].	a procedure is developed for estimation of glucose-6-phosphate and 6-phosphogluconate dehydrogenases activity in human erythrocytes. the procedure involved polarographic registration of oxygen consumption, which is directly related to content of nadph formed, in a coupled enzymatic system containing peroxidase and mn2+.	1986	3811277
peptococcaceae bacteremia.	bacteremia with peptococcaceae is an uncommon clinical manifestation of infection with this family of microorganisms. a 20-month review of 12 patients with bacteremic infections due to anaerobic gram-positive cocci revealed that obstetrical patients during the peripartum period constitute the group at greatest risk for the development of such infections. eight of the 12 patients were young women hospitalized on the obstetrical service. seven patients had postpartum endometritis and one patient h ...	1986	3956135
spinal cord serotonin release and raised blood pressure after brainstem kainic acid injection.	the recently developed technique of in vivo dialysis has permitted us to make direct measurements of serotonin release in a specific region of the spinal cord and to relate this to changes in blood pressure elicited by chemical stimulation of the brainstem. in the present experiments we have shown that chemical stimulation of bulbospinal neurons in the region of the b3 cell group in the ventromedial medulla, causes an increase in the release of serotonin in the thoracic spinal cord and that this ...	1986	3697690
[morphological changes caused by adrenalectomy in rabbits].		1986	3019628
ampicillin/sulbactam versus metronidazole-gentamicin in the treatment of soft tissue pelvic infections.	the clinical efficacy and safety of ampicillin/sulbactam versus metronidazole-gentamicin were evaluated in a comparative, randomized, prospective study. forty-four patients were enrolled: 22 received the ampicillin/sulbactam regimen, and 22 received the metronidazole-gentamicin combination. there were 33 cases of severe acute pelvic inflammatory disease, two tuboovarian abscesses, five cases of endomyometritis, and two cases of posthysterectomy pelvic cellulitis. aerobic and anaerobic cultures f ...	1987	3030109
surgical cannulation of a hepatic vein in dairy cows utilizing diagnostic ultrasound.	use of a diagnostic ultrasonography unit in the placement of hepatic vein catheters is described. diagnostic ultrasound proved to be a safe and rapid means of identifying the hepatic venous circulation. it provided a clear visual guide in an otherwise blind surgical procedure and allowed for a precise determination of catheter placement. thus, the method could be used to advantage in other types of catheterizations. use of ultrasonography reduced the duration of surgery by 50 to 75%, thereby min ...	1987	3294948
[studies on antimicrobial concentrations of flomoxef in serum, pelvic dead space exudate, and pelvic organs/tissues].	to women undergoing radical and total hysterectomy, flomoxef (fmox, 6315-s) in a dose of 2 g was administered by intravenous drip infusion over 1 hour and drug concentrations in serum and pelvic dead space exudate as well as pelvic organs/tissues were determined over time. the following results were obtained: 1. serum concentrations of fmox after intravenous infusion showed the peak value of 92.86 +/- 17.05 micrograms/ml at the end of infusion and then gradually decreased to 29.00 +/- 10.49 micr ...	1987	3444023
demonstration of locally synthesized borrelia antibodies in cerebrospinal fluid.	antibodies against borrelia burgdorferi develop slowly. low titers can be anamnestic. in order to prove the etiology of manifestations of erythema chronicum migrans disease of the nervous system like meningopolyneuritis garin-bujadoux-bannwarth or progressive borrelia encephalomyelitis we used the elisa to measure specific igg antibodies against ixodes ricinus borrelia per microgram igg in serum and cerebrospinal fluid. with the mentioned method we were able to demonstrate locally synthesized an ...	1987	3591093
identification of anaerobic bacteria using high-performance liquid chromatography.	20 species of the genera clostridium, bacteroides, fusobacterium and the type strains of peptostreptococcus anaerobius, peptococcus asaccharolyticus, veillonella parvula and propionibacterium acnes were examined for the production of volatile (vfa) and nonvolatile (nvfa) short-chain fatty acids using high-performance liquid chromatography (hplc) with a column for organic acids (aminex hpx-87h). 10 min are needed for sample preparation and the vfa and nvfa were detected simultaneously in a single ...	1987	3673341
effects of ru 486 on the mechanical properties of rabbit fallopian tubes.		1988	3377712
recovery of anaerobic bacteria from clinical specimens in 12 years at two military hospitals.	examination of 15,844 clinical specimens submitted over 12 years (1973 to 1985) to the anaerobic microbiology laboratories in two military hospitals demonstrated the recovery of anaerobic bacteria in 4,458 (28.1%) specimens. the specimens yielded 6,557 anaerobic isolates (1.47 isolates per specimen). bacteroides spp. accounted for 43% of all isolates; anaerobic gram-positive cocci, 26%; clostridium spp., 7%; and fusobacterium spp., 4%. bacteroides spp. predominated in abscesses, obstetrical and ...	1988	3384929
evidence of estrogen receptors in normal human osteoblast-like cells.	in seven strains of cultured normal human osteoblast-like cells, a mean of 1615 molecules of tritium-labeled 17 beta-estradiol per cell nucleus could be bound to specific nuclear sites. the nuclear binding of the labeled steroid was temperature-dependent, steroid-specific, saturable, and cell type-specific. these are characteristics of biologically active estrogen receptors. pretreatment with 10 nanomolar estradiol in vitro increased the specific nuclear binding of progesterone in four of six ce ...	1988	3388021
structure of ferredoxin i from azotobacter vinelandii.	the structure of azotobacter vinelandii ferredoxin i (av fdi, 106 amino acids) has been redetermined, based on x-ray diffraction data from tetragonal crystals of the native protein and two heavy atom derivatives. the current model differs greatly from the one previously reported and is in agreement with arguments based on various spectroscopic and other methods. the unit cell parameters are a = b = 55.62 a and c = 95.51 a, whereas the space group was found to be p4(1)2(1)2 instead of p4(3)2(1)2. ...	1988	3422475
piezoelectric polymer curvature sensor for measurement of regional curvature radius of lv wall.	to evaluate regional myocardial function, we developed a curvature sensor for direct and instantaneous measurement of the regional curvature radius of the lv wall. the sensor is a bimorph of two sheets of thin piezoelectric polymer film. the relation between output voltage of the sensor and the reciprocal of the known curvature radius has been shown to be linear. in anesthetized dogs, we inserted the curvature sensor into the subepimyocardium with a specially designed introducer. special care wa ...	1988	3364584
influence of inoculation dose, inoculation schedule, chicken age, and host genetics on disease susceptibility and development of resistance to eimeria tenella infection.	various parasite- and host-related factors influencing disease susceptibility and development of protective immunity against eimeria tenella infection were investigated in two inbred strains of chickens. chickens that received a primary inoculation of 10(3), 10(4), or 10(5) oocysts showed a significant reduction in packed cell volume and produced significantly more oocysts than chickens inoculated with fewer oocysts. younger chickens were as susceptible as older chickens to identical parasite do ...	1988	3264150