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[non-puerperal mastitis. etiology, clinical aspects and therapy].non-puerperal mastitis was diagnosed in 79 patients (aged 12-77 years) over the years 1974-1984. malignant neoplasm was not present. bacterial infection in the region of the areola was the most frequent finding (40%), followed by abacterial inflammation without involvement of the nipples (29%). the other cases, bacterial or nonbacterial, occurred at different sites. the histological picture or clinical features of an increased secretory activity of the mammary gland (galactorrhoea, mastodynia) i ...19854038482
qualitative and quantitative changes of the vaginal microbial flora during the menstrual cycle.knowledge of the normal vaginal microbial flora is essential in understanding the etiology of female genital tract infections. this study was done to investigate both qualitative and quantitative differences that occur during the menstrual cycle. we studied 34 reproductive age women longitudinally. quantitative cultures were obtained during menses and following the cessation of menses. a greater variety of organisms were found during menstruation. the effect was not selective with respect to aer ...19854051082
in vitro effects of sulphasalazine, azodisal sodium, and their metabolites on clostridium difficile and some other faecal bacteria.the effects of sulphasalazine (sasp), azodisal sodium (ads), and their metabolites were tested in vitro on aerobic and anaerobic faecal bacterial strains. sulphapyridine (sp) had a mild to moderate effect on escherichia coli and streptococcus faecalis. sasp also had a growth-inhibitory effect on strep. faecalis. the other substances had no effect on the aerobic strains. sasp, sp, 5-aminosalicylic acid, and, to a certain extent, n-acetyl-5-aminosalicylic acid exerted a growth-inhibitory effect on ...19852862692
effect of sodium polyanetholesulfonate and gelatin on the recovery of gardnerella vaginalis from blood culture media.sodium polyanetholesulfonate (sps) is used as a routine supplement to blood culture media to enhance recovery of microorganisms, but it inhibits the growth of peptostreptococcus anaerobius, neisseria meningitidis, neisseria gonorrhoeae, and streptobacillus moniliformis. comparative clinical blood culture studies at the university of colorado hospital suggested that sps also inhibits the growth of gardnerella vaginalis. we inoculated 16 blood culture isolates of g. vaginalis into 11 blood culture ...19852987298
comparative evaluation of three identification systems for anaerobes.the accuracy of two new 4-h identification systems for anaerobes, the an-ident (analytab products, plainview, n.y.) and the rapid ana (innovative diagnostic systems, inc., atlanta, ga.) was compared with that of the api 20a system (analytab products). a total of 132 clinical isolates were tested in each of the three systems. the overall accuracies at the genus and species level for the three systems were: api 20a, 68.9 and 56.8%, respectively; an-ident, 90.2 and 73.5%; and rapid ana, 93.9 and 81 ...19852862161
comparative in-vitro activity of sch 34343 and other antimicrobial agents against anaerobic bacteria.the activity of sch 34343 was determined against 575 strains of anaerobic bacteria by an agar-dilution method. its activity was compared with that of benzylpenicillin, piperacillin, cefoxitin, imipenem, clindamycin, metronidazole, chloramphenicol, vancomycin, fusidic acid and bacitracin. sch 34343 and imipenem were the most active agents tested. based on these results, sch 34343 appears to be a promising antimicrobial agent for anaerobic infections and warrants further clinical investigations.19852863246
enzymatic reduction of sennidin and sennoside in peptostreptococcus intermedius.peptostreptococcus intermedius, one of the dominant bacteria of human intestine, reduced sennidin and sennoside to rheinanthrone and 8-glucosyl-rheinanthrone, respectively, and these reduction rates were stimulated by the addition of nicotinamide adenine dinucleotide (reduced form) (nadh), flavin adenine dinucleotide (fad) and glucose. the reduction was accelerated by removing oxygen from the incubation tubes, which indicates the inhibition of the reduction by o2. thus, for the maximal activity, ...19853841560
predominant obligate anaerobes in human carious dentin.with the adoption of an anaerobic glove box system, we isolated and identified the predominant micro-organisms in lesions of carious dentin from four permanent third molars. the overwhelming majority of the micro-organisms isolated from both shallow and deep layers of carious dentin were obligate anaerobes, suggesting that conditions in the dentin were strictly anaerobic. the predominant obligate anaerobes were gram-positive rods which were identified as members of the following genera: propioni ...19853861648
anaerobic infections. part i. 19853862516
antibiotic susceptibilities of periodontal bacteria. in vitro susceptibilities to eight antimicrobial agents.in vitro susceptibilities of 369 to 966 bacterial isolates from periodontal lesions to eight antibiotics were determined by agar dilution technique as a means of determining which antimicrobial agents were inhibitory for bacteria frequently associated with destructive periodontal diseases. although most bacteria were relatively susceptible to the penicillins, greater activity was generally noted with amoxicillin than with either penicillin or ampicillin with the exception of selenomonas sputigen ...19853866054
a microaerophilic coccus in pyogenic infections of ruminants.pyogenic infections of cattle, sheep and goats were examined for the presence of a gram positive bacterium that has been designated "microaerophilic coccus" by other workers. the bacterium was found to be involved in a range of disease processes, including foot and soft tissue abscesses, mastitis, pericarditis and pyometra in cattle, joint and foot abscesses in sheep and foot abscesses in goats. the characteristic feature of the bacterium was its satellitic growth around colonies of other organi ...19853888166
comparative clinical evaluation of imipenem/cilastatin vs. cefotaxime in treatment of severe bacterial infections.adult patients with severe bacterial infections caused by organisms susceptible to imipenem and cefotaxime were given either imipenem/cilastatin sodium (mk0787/mk0791) or cefotaxime as a part of a multiclinic randomized study to evaluate the effectiveness, safety, and tolerability of imipenem/cilastatin. clinical diagnoses included bacteremia, urinary tract infection, osteomyelitis, mediastinitis, lower respiratory tract infection, and soft tissue infection. efficacy was evaluated for 10 patient ...19853901208
comparison of the pras ii, an-ident, and rapid-ana systems for identification of anaerobic bacteria.two rapid systems for the identification of anaerobes were compared to a conventional growth system aided by a computer. the rapid systems (an-ident and rapid-ana) are non-growth-dependent micromethods that identify anaerobes in 4 h by the action of various constitutive enzymes on chromogenic substrates. the organisms tested were 98 anaerobes, most of which were clinical isolates. the an-ident system identified 76 of these to species level and 86 to genus level; the rapid-ana system correctly id ...19853894412
bactericidal effects of photoradiation therapy with hematoporphyrin derivative.hematoporphyrin derivative (hpd) localizes selectively in malignant and rapidly metabolizing tissues and undergoes a cytotoxic reaction when exposed to light of a specific wavelength. hpd has been studied extensively with regard to the diagnosis and treatment of tumors but not with regard to bactericidal activity. this investigation assessed the effect of light-activated hpd on various microorganisms, on human polymorphonuclear leukocytes, and on the interactions of polymorphonuclear leukocytes ...19853155547
influence of dietary fiber on the intestinal environment. 19853001739
anaerobic peptostreptococcal keratitis. 19853895968
imipenem/cilastatin in the treatment of obstetric and gynecologic infections: a review of worldwide experience.worldwide experience with imipenem/cilastatin in the treatment of 72 patients with a variety of obstetric and gynecologic infections is reviewed. clinical cure or improvement occurred in 97% of 72 assessable patients. the most common etiologic pathogens were escherichia coli, group b streptococci, neisseria gonorrhoeae, staphylococcus epidermidis, enterococci, bacteroides bivius, bacteroides species, bacteroides fragilis, and peptostreptococcus. of the recovered pathogens, 99.5% were susceptible ...19853901216
peritonsillar abscess: bacteriological evaluation.the pus from a series of 41 peritonsillar abscesses was examined bacteriologically. in the majority of the abscesses a mixed bacterial flora was found. the specimens yielded 0-7 different bacterial species per abscess (mean 3.0). one species alone was isolated only in five cases (12.5%). both anaerobic and aerobic bacteria were isolated from the specimens of 25 patients (61%), only anaerobes from two specimens (4.9%), and only facultative bacteria from 12 specimens (29%). beta haemolytic strepto ...19853891156
analysis of gram-positive anaerobic cocci in oral, fecal and vaginal flora. 19854043069
moxalactam versus clindamycin plus tobramycin in the treatment of obstetric and gynecologic infections.the clinical efficacy of moxalactam versus clindamycin/tobramycin was evaluated in a comparative, randomized, prospective study. sixty patients were treated: 30 with moxalactam and 30 with clindamycin/tobramycin. there were 15 cases of tuboovarian abscess, 36 cases of severe pelvic inflammatory disease with peritonitis, eight cases of endomyometritis, and one wound abscess. aerobic and anaerobic cultures from the sites of infection yielded 441 microorganisms from 53 patients; an average of 8.3 b ...19853895947
[comparative study in vitro on the activity of 7 antibiotics against anaerobic bacteria from hospital isolates].one hundred and ninety-four anaerobic bacteria isolated from clinical sources were tested by agar dilution technique against seven antimicrobial agents. a comparison with international literature, generally used for selecting appropriate antimicrobial drug showed concordant patterns of susceptibility in the case of cocci gram-positive, clostridia, fusobacterium and other species of bacteroides excluded b. fragilis group. for these strains we did not found any particular problem in the choice of ...19854074510
a sodium ion gradient as energy source for peptostreptococcus asaccharolyticus.the determination of enzymatic activities in cell-free extracts of acidaminococcus fermentans and peptostreptococcus asaccharolyticus led to a refined scheme for the pathway of glutamate fermentation via (r)-2-hydroxyglutarate to acetate and butyrate. from the ratio of these products the amount of atp generated by substrate level phosphorylation was calculated. growth experiments with the organisms including clostridium symbiosum and clostridium tetanomorphum indicated that a sodium gradient con ...19854037980
effect of aspoxicillin on anaerobic bacteria.aspoxicillin (aspc), a semisynthetic penicillin has a broad spectrum of antibacterial activities against gram-positive and gram-negative anaerobic bacteria. its in vitro antibacterial activity was less than those of cefoxitin against peptostreptococcus and veillonella, but was significantly high against bacteroides fragilis, one of the most clinically important anaerobe. the therapeutic and/or protective effect of aspc in experimental subcutaneous abscess or experimental intraabdominal mixed inf ...19853850132
survival analysis of periodontal sites before and after periodontal therapy.periodontal diseases appear to progress with bursts of destructive activity at individual sites. one effect of treatment might be to diminish the frequency of such bursts. survival analysis was employed to seek such effects on the periodontal sites of 16 individuals with prior evidence of destructive periodontal disease. the subjects were monitored at bi-monthly intervals and actively breaking down sites were detected using attachment level measurements and the tolerance method of analysis. when ...19853860518
mutagenic activation of biliary metabolites of 1-nitropyrene by intestinal microflora.to investigate the modifying role of intestinal microflora in the metabolism of chemical carcinogens in vivo, we subjected bile from fischer rats treated per os with chemical carcinogens and related compounds to a mutagenicity assay in the presence and absence of a cell-free extract from human feces. a mixture of the bile sample and potassium phosphate buffer was incubated in the presence or absence of human cell-free fecal extract and then further incubated with a bacterial suspension of salmon ...19853982438
purification and characterization of hyaluronidase from oral peptostreptococcus species.hyaluronidase was purified to apparent homogeneity from the spent medium of peptostreptococcus sp. strain 84h14s. the enzyme was purified 310-fold by ethanol precipitation, gel chromatography, and cation-exchange chromatography with a recovery of 42% of the original activity in the culture medium. the molecular weight of the purified enzyme was estimated to be 160,000 by gel filtration with sephacryl s-300. like bacterial mucopolysaccharidases of other sources, the enzyme carried out an eliminat ...19853881352
anaerobic infections of burns.from august 1980 to june 1982, 102 burn wound specimens taken from 34 patients were studied for anaerobic cultures. fifteen instances (14.7 per cent) from 8 patients were positive and altogether 12 species were found. the predominant anaerobes were bacteroides melaninogenicus, peptococcus, bacteroides fragilis, and other strains of bacteroides and peptostreptococcus. they were mostly discovered in electric burn wounds and burn wounds affecting the perianal and oral areas. wounds with anaerobic i ...19853886089
beta-glucuronidase activities of intestinal bacteria determined both in vitro and in vivo in gnotobiotic rats.the beta-glucuronidase activities of bacterial strains isolated from the rat intestinal tract were studied both in vitro in culture media and in vivo in the intestinal contents of gnotobiotic rats. only 50 of 407 strains tested were found to be positive in vitro. they belonged to the three genera clostridium, peptostreptococcus, and staphylococcus. the in vitro-negative strains were also negative in vivo. the beta-glucuronidase activities of the beta-glucuronidase activities of the positive stra ...19853994372
evaluation of the rapid-ana system for identification of anaerobic bacteria of veterinary origin.this study evaluated the ability of the rapid-ana system (innovative diagnostic systems, inc., atlanta, ga.) to accurately identify a spectrum of freshly isolated veterinary anaerobes. a total of 183 isolates were tested and included 7 actinomyces spp., 53 bacteroides spp., 32 clostridium spp., 2 eubacterium spp., 65 fusobacterium spp., 1 peptococcus spp., 22 peptostreptococcus spp., and 1 propionibacterium spp. all isolates were initially identified by conventional biochemical testing and gas-l ...19853905854
[etiology of bacterial vaginosis (non-specific vaginitis)].56 women who were diagnosed bioclinically as having a bacterial vaginal infection were studied, as were 35 women as a control group. the study was a semi-quantitative analysis of the vaginal bacterial flora, both aerobic and anaerobic. it shows that gardnerella vaginalis and anaerobic bacteria such as peptococcus, peptostreptococcus, bacteroïdes, veillonella and mobiluncus were associated in a statistically significant way with bacterial vaginitis. on the other hand lactobacilli were less freque ...19854078248
the classification and identification of the anaerobic gram-positive cocci.the approved lists of bacterial names (1980) includes the following peptococcus and peptostreptococcus species pathogenic for man: peptococcus magnus, p. prevotii, p. asaccharolyticus, peptostreptococcus anaerobius, p. micros and p. productus. a presumptive identification of these species can be made on the basis of cellular morphology, susceptibility to novobiocin, characterization of metabolic end products, fermentation of glucose and lactose, and production of indol. capnophilic and microaero ...19853906854
respiratory infections: clinical evaluation.a review of clinical studies of piperacillin shows that it is valuable for the treatment of respiratory infections due to enterobacteriaceae, pseudomonas sp, anaerobes, and mixed flora including anaerobes. various studies of a total of 420 patients treated with piperacillin for lower respiratory tract infections found that 97% of the patients were cured or markedly improved. piperacillin has also been found as effective as combination therapy (gentamicin or tobramycin plus carbenicillin or ticar ...19853907845
[a comparative study of the various organisms causing salpingitis and their potential presence in iud wearers].a prospective study was undertaken to compare the enbdometrial and vaginal bacteria of a continuous series of 283 patients who had iuds removed between june 1, 1979 and june 1, 1983, by the same clinician. measures were taken to avoid contamination of the iud upon removal by the vaginal bacteria, but as a practical matter it was impossible to avoid all contact. the iuds involved were multiload copper 250 or 375, gyne t and t 200, nova t, and dimelys. the number of positive cultures from the ...198512339972
clinical and microbiological characterization of patients with nonspecific vaginosis associated with motile, curved anaerobic rods.the vaginal secretions of 20 normal control subjects and 21 patients with motile, curved anaerobic rods were cultured for aerobic and anaerobic bacteria, chlamydia trachomatis, herpes simplex virus, and trichomonas vaginalis. extensive histories and physical examinations of the patients and microscopic appearance and gas-liquid chromatography patterns of vaginal secretions were compared between the two groups. the patients who had motile rods in their vaginal secretions more frequently presented ...19846144713
ceftriaxone: therapeutic results in various infections and kinetic studies.ceftriaxone (ro 13-9904, rocephin) was given to 67 patients suffering from 74 various infections. patients had infections of the urinary tract (36), soft tissue phlegmon (12), infections of the respiratory tract (13), osteomyelitis (7), abscesses (5) and meningitis (1). infecting organisms were e. coli (26), proteus spp. (20), p. aeruginosa (7), h. influenzae (6), enterobacter spp. (6), k. pneumoniae (3), c. freundii (2), s. marcescens (1), s. aureus (4), s. pneumoniae (2), peptostreptococcus sp ...19846329238
role of anaerobic bacteria in bite-wound infections.the etiologic agents usually involved in wound infections due to human or animal bites are the aerobic skin flora of the victim, e.g., staphylococcus aureus, and/or the aerobic oral flora of the biter, e.g., pasteurella multocida. while anaerobic bacteria are predominant in the normal oral flora of humans and animals, their importance in the pathogenesis of bite-wound infections has not been stressed. most investigators in this field have either not cultured these wounds for anaerobic bacteria o ...19846372026
antimicrobial susceptibility of anaerobic bacteria in sweden in 1983.this review is concerned with anaerobic bacteria and antimicrobial susceptibility. data on the susceptibility to different antimicrobials of anaerobic isolates in sweden during 1983 is presented. among the different bacterial groups, clostridium perfringens, propionibacteria, anaerobic cocci and curved rods were found to be susceptible to beta-lactam antibiotics, clindamycin and chloramphenicol. c. difficile was susceptible to benzylpenicillin and imipenem, but resistant to cefoxitin. most c. di ...19846100139
isolation of obligate anaerobic bacteria from bovine abscesses in sites other than the liver.a survey in japan showed that of 2036 slaughtered cattle 58(3%) had abscesses in sites other than the liver. in 21 of the affected animals the lesions were pulmonary and in 32 abdominal (excluding hepatic); in five animals the lesions were found elsewhere (muscle 2, skin 2, bone 1). nineteen (33%) of the 58 cattle also had abscesses in the liver. obligate anaerobes alone were isolated from 17(29%) of the affected animals (fusobacterium necrophorum 14, bacteroides spp. 2, peptostreptococcus anaer ...19846389873
[anaerobic microflora of the contents of the upper maxillary sinuses in patients with chronic sinusitis]. 19846401097
[bacterial flora of the urogenital tract in men with non-gonorrheal urethritis]. 19846144143
a kinetic study of the oxidative deamination of l-glutamate by peptostreptococcus asaccharolyticus glutamate dehydrogenase using a variety of coenzymes.the nad+-specific glutamate dehydrogenase from peptostreptococcus asaccharolyticus follows michaelis-menten kinetics in contrast to the enzyme from several other sources, and thus gives linear double-reciprocal plots of initial-rate data. the initial-rate parameters have been determined for this bacterial dehyrogenase in the direction of oxidative deamination. the use of alternative coenzymes leads to some conclusions about the order of substrate addition. an investigation of the ph dependence o ...19846148240
[a case of septic pulmonary emboli due to peptostreptococcus associated with liver abscess]. 19846434659
[in vitro activity and clinical evaluation of ceftriaxone in the field of obstetrics and gynecology].in vitro activity of ceftriaxone (ctrx) was examined by agar plates dilution method against 398 strains isolated from the infections in the field of obstetrics and gynecology. mic90 of ctrx against staphylococcus (107 strains), e. coli (54 strains), k. pneumoniae (27 strains), peptococcus and peptostreptococcus (106 strains) and bacteroides (104 strains) was more than 100 micrograms/ml, less than 0.20 micrograms/ml, less than 0.20 micrograms/ml, 6.25 micrograms/ml and 50 micrograms/ml, respectiv ...19846098728
production of menaquinones by intestinal anaerobes.ninety intestinal organisms (71 isolates from fecal samples of neutropenic patients with cancer or from various sites in patients with intraabdominal infections and 19 control strains) were examined by reverse-phase thin-layer chromatography for their ability to produce menaquinones in vitro. menaquinones were found in all of 24 organisms of the bacteroides fragilis group. two other bacteroides species, bacteroides disiens and bacteroides bivius, also produced menaquinones. a single isolate of b ...19846470528
anaerobic ureolytic bacteria from caecal content and soft faeces of rabbit.forty strains of ureolytic bacteria were isolated from the caecal content and soft faeces of seven rabbits by the anaerobic roll tube method and were characterized. the isolates were identified with clostridium coccoides, cl. innocuum, peptostreptococcus productus, p. micros, peptococcus magnus, fusobacterium russii and fusobacterium sp. urease activity of representative strains of the various species was also determined. the study indicated that strongly-ureolytic anaerobic bacteria are present ...19846490567
induction in rats of serum agglutinins to eubacterium, peptostreptococcus and coprococcus species by the faecal flora from patients with crohn's disease and healthy subjects.sera from patients with crohn's disease more often than those from other subjects contain agglutinins against anaerobic coccoid rods, identified as peptostreptococcus productus, eubacterium contortum (two strains) and coprococcus comes. the presence of antigens of the four coccoid rods in faeces of patients with crohn's disease and healthy subjects was investigated by inoculation of rats with faecal suspensions. antigens of the coccoid rods were detected in faeces from both patients with crohn's ...19846501880
characterization of peptostreptococcus asaccharolyticus glutamate dehydrogenase purified by dye-ligand chromatography.glutamate dehydrogenase (l-glutamate:nad+ oxidoreductase (deaminating); ec 1.4.1.2) has been purified from peptostreptococcus asaccharolyticus in a single step using dye-ligand chromatography. the enzyme (gdh) was present in high yields and was stabilized in crude extracts. a subunit molecular weight of 49000 +/- 500 was determined by sds polyacrylamide gel electrophoresis and six bands were obtained after cross-linking the subunits with dimethyl suberimidate. this bacterial gdh was predominantl ...19846502134
peptostreptococcus productus strain that grows rapidly with co as the energy source.anaerobic bacteria were enriched with a sewage digestor sludge inoculum and a mineral medium supplemented with b-vitamins and 0.05% yeast extract and with a 50% co-30% n2-20% co2 (2 atm [202 kpa]) gas phase. microscopic observation revealed an abundance of gram-positive cocci, 1.0 by 1.4 micron, which occurred in pairs or chains. the coccus, strain u-1, was isolated by using roll tubes with co as the energy source. based on morphology, sugars fermented, fermentation products from glucose (h2, ac ...19846430231
[vertebral osteomyelitis caused by anaerobes. a new case]. 19846531485
gas-producing brain abscess due to peptostreptococcus. 19846204240
experimental otitis media in guinea pigs. serum antibody response to bacteroides fragilis, propionibacterium acnes and peptostreptococcus anaerobius upon challenge.the production of antibodies in serum was studied after inoculation of bacteroides fragilis, propionibacterium acnes or peptostreptococcus anaerobius into the middle ear of guinea pigs. inoculation of b. fragilis resulted in igg and igm serum antibodies at an early stage of the infection, while iga antibodies were seldom detected and at a later stage. in contrast, inoculation of p. acnes or p. anaerobius induced no serum antibody response. of these three species b. fragilis induced the most inte ...19846598260
bacteriology of chronic tonsillitis in young adults.tonsils were obtained from 22 young adults (mean age, 23 years) suffering from chronic tonsillitis. mixed aerobic and anaerobic flora was obtained from core tonsillar cultures in all patients, yielding an average of 9.0 isolates (5.3 anaerobes and 3.7 aerobes) per specimen. the predominant anaerobic isolates were bacteroides sp, fusobacterium sp, and gram-positive cocci. the predominant aerobic isolates were alpha-hemolytic streptococci, staphylococcus aureus, branhamella catarrhalis, beta-hemol ...19846334513
peptostreptococcal empyema in akinetic mutism.peptostreptococcal empyema occurred in a 54-year-old man who had suffered from diabetes mellitus and had been in akinetic mutism. ultrasonic tomogram was useful to detect the space of empyema and to identify the decrease in volume of the empyema space with treatment.19846492491
bactericidal and cytotoxic effects of hypothiocyanite-hydrogen peroxide mixtures.lactoperoxidase catalyzes the oxidation of thiocyanate by hydrogen peroxide into hypothiocyanite, a reaction which can protect bacterial and mammalian cells from killing by hydrogen peroxide. the present study demonstrates, however, that lactoperoxidase in the presence of thiocyanate can actually potentiate the bactericidal and cytotoxic effects of hydrogen peroxide under specific conditions, such as when hydrogen peroxide is present in the reaction mixtures in excess of thiocyanate. the toxic a ...19846724690
osteomyelitis in dogs and cats caused by anaerobic bacteria.localised osteomyelitis was diagnosed in 2 dogs and 2 cats. the disease was caused by fight wounds in 3 cases. radiographic examination demonstrated a circumscribed zone of cortical bone lysis, sequestra and periosteal new bone. each case was treated surgically by sequestrectomy and debridement. infection was due mainly to anaerobic bacteria. the pathogenic bacteria isolated from the lesions of dogs were actinomyces viscosus, fusobacterium nucleatum and bacteroides spp, and from the lesions in c ...19846732670
radiological case of the month. anaerobic pyopneumopericardium. 19846741898
pathogenicity of anaerobic gram-positive cocci.the pathogenicity of 20 strains of facultative or anaerobic gram-positive cocci (agpc) was investigated by injecting them alone or mixed with other flora into mice, utilizing the subcutaneous abscess model. abscesses induced by a mixture of two organisms were uniformly larger than those induced by single organisms. the relationships among seven agpc strains, eight aerobes, and two bacteroides spp. were determined by treating the infected animals with antibiotics and observing the effect of thera ...19846746091
anaerobic bacteria isolated from foot infections in diabetic patients: in vitro susceptibility to nine antibiotics.twenty-nine isolates of anaerobic bacteria from foot infections in diabetic patients were tested, using the agar dilution method, for in vitro susceptibility to nine antimicrobial agents. imipenem and metronidazole showed excellent activity; clindamycin and chloramphenicol were next in terms of efficacy. cefoxitin and moxalactam demonstrated good activity, although three isolates of the bacteroides fragilis group were resistant to moxalactam. penicillin g, cefotaxime, and cefoperazone showed poo ...19846467279
anaerobic oral and dental infection.anaerobes make up a significant part of the oral and dental indigenous and pathogenic flora. their role in periodontal disease, root canal infections, infections of the hard and soft oral tissue, as well as their importance as foci for disseminated infectious disease is well established. despite the ubiquitous involvement of bacteria, significant progress in our understanding of specific microbial etiologies has occurred only in the past decade. estimates of the number of species recovered from ...19846372018
diffuse sclerosing osteomyelitis of the mandible.the medical care of patients with diffuse sclerosing osteomyelitis (dso) of the mandible has been unsatisfactory. the main reasons for this have been insufficient knowledge of the disease and its natural history, difficulties in establishing the correct diagnosis, and the unknown etiology. the clinical features and natural history of the disease were studied by means of repeated radiographic and scintigraphic recordings. the biopsy technique was improved by using a slowly rotating coarse trepan ...19846437998
surgical management of piriform sinus fistula.four cases of acute cervical abscess resulting from infection through the left piriform sinus fistula are described. the fistula seems to be related to the cause of infection in many of previously reported cases of acute suppurative thyroiditis or "recurrent lateral cervical fistula." complete removal of the fistula is essential to a permanent cure, and the guide of a fogarty catheter through the fistula is useful at operation for patients with repeated infection.19846465693
pectinolytic enzymes of oral spirochetes from humans.five strains of obligately anaerobic, pectin-fermenting spirochetes were isolated from the subgingival plaque of humans. the strains produced two extracellular enzymatic activities that functioned in pectin degradation. one of these enzymatic activities was pectin methylesterase (ec 3.1.1.11), and the other was pectate lyase (ec 4.2.2.2) of the endo type. the data indicate that the cumulative action of these two enzymatic activities brought about depolymerization of pectin in spirochete cultures ...19846383218
anaerobes as normal oral flora.the human mouth provides a suitable habitat for numerous bacterial genera. anaerobic genera or genera that include anaerobic members found in the oral cavity are actinomyces, arachnia, bacteroides, bifidobacterium, eubacterium, fusobacterium, lactobacillus, leptotrichia, peptococcus, peptostreptococcus, propionibacterium, selenomonas, treponema, and veillonella. the incidence of anaerobes varies with age of the individual and with specific sites sampled. in edentulous infants, the incidence of a ...19846372039
clinical efficacy of cefoperazone in obstetric and gynecologic infections.compared with second-generation cephalosporins, cefoperazone, a third-generation cephalosporin, has demonstrated an extended antibacterial spectrum. in addition, it has a long serum half-life and requires only twice-a-day administration. in this study, 66 patients with clinically diagnosed obstetric and gynecologic infections were treated with cefoperazone, and 61 were evaluated for efficacy. an overall cure rate of 93% (57 patients) was achieved. in view of the excellent clinical response and c ...19846383613
[growth of anaerobes of the upper small intestine using the glovebox technic].with 80 patients a microecologic analysis of the duodenal flora has been performed. due to the optimal cultivation technique with the glove-box more frequently as supposed till now, anaerobes with partial high numbers could be detected, and classified to different types of dysbiosis. a striking role among the isolated and classified gram-positive roots play the genera eubacterium, propionibacterium, actinomyces, and bifidobacterium. peptococcus and peptostreptococcus are to be found frequently, ...19846387500
microorganisms isolated from infected aural fistulas.aural fistula is a congenital deformity of the external ear relatively common in orientals and rare in caucasians. suppuration tends to occur, and chemotherapy rather than surgical drainage should be attempted. however, the lack of information concerning infected aural fistulas has made appropriate chemotherapy difficult. microorganisms isolated from 13 cases of infected aural fistulas were studied from january 1981 to december 1982. six species and 22 strains were isolated; nonsporeforming facu ...19846492970
intestinal water-soluble mucins in germfree, exgermfree and conventional animals.water-soluble intestinal mucins were investigated in germfree (gf), exgermfree (exg) and conventional (conv) rats and in gf and conv mice. after agar gel electrophoresis, all gf animals had similar specific band patterns demonstrated by pas and toluidine blue. these patterns, never seen in conv animals, disappeared in gf animals infected either with intestinal contents from conv rats or mono-infected with a mucin converting microorganism, labelled peptostreptococcus n. the intestinal microflora ...19846516849
in vitro susceptibility of anaerobic bacteria to ciprofloxacin (bay o 9867).about 80% of 70 clinical isolates of bacteroides fragilis were inhibited by 4 micrograms of ciprofloxacin (bay o 9867) per ml. the 90% mic of ciprofloxacin was 8 micrograms/ml for other bacteroides species, 2 micrograms/ml for peptococcus species, 8 micrograms/ml for peptostreptococcus species, and 16 micrograms/ml for clostridium and eubacterium species.19846517561
[recently detected organisms from oral infectious diseases and their antimicrobial sensitivity]. 19846597502
suppurative peritonitis in cats associated with anaerobic bacteria. 19846529399
b-lymphocyte blastogenesis in response to periodontitis-associated bacteria. kinetics and proportion of total response.a role for activated b-lymphocytes in mediating the initiation and/or progression of periodontal diseases has been proposed in previous work. the present study was conducted to: (1) assess the proportion of total lymphocyte blastogenic response to selected oral bacteria which is composed of t-cell and b-cell activation, respectively, and (2) to determine whether different kinetic patterns exist for the t-cell vs. the b-cell responses to these bacteria. using lymphocyte rosetting and culturing te ...19846610740
agglutinins to anaerobic bacteria in crohn's disease and in indian patients with diarrhoea.agglutinins to certain species of eubacterium and peptostreptococcus have been reported in sera from a high proportion of patients with crohn's disease. because this might be a non-specific finding common to patients with diarrhoea associated with damaged intestinal mucosa, we have compared the incidence of such agglutinins in patients with crohn's disease with that seen in patients in north-east india with acute or chronic diarrhoea. the incidence of agglutinins in crohn's disease was 44%, comp ...19846708086
tubo-ovarian abscess: contemporary approach to management.two hundred and thirty-two patients with tubo-ovarian abscesses (toas) were evaluated. ruptured toas were documented in seven (3%) of the patients. one hundred and seventy-five patients with toas were treated with antibiotics alone; for 15 of these patients, toas were confirmed by laparoscopy. the remaining 57 patients required surgical intervention: drainage (five patients), unilateral salpingo-oophorectomy (19) and total abdominal hysterectomy and bilateral salpingo-oophorectomy (33). a unilat ...19836635426
[experimental and clinical evaluation of cefroxadine in the field of obstetrics and gynecology].cefroxadine (cxd) was studied in the field of obstetrics and gynecology and the following results were obtained. mic values of cxd against 55 clinical isolates were investigated, from 6.25 to 12.5 micrograms/ml for 8 strains of e. coli, 6.25 micrograms/ml for 3 strains of k. pneumoniae and from 0.39 to 3.13 micrograms/ml for 9 strains of anaerobes such as peptostreptococcus and peptococcus in 10(6) cells/ml. transfer of cxd into intrapelvic organ such as uterus, uterine tube and ovary after the ...19836655868
selected bacterial antibodies in crohn's disease and ulcerative colitis.agglutinins to four strains of anaerobic gram-positive coccoid rods (species of eubacterium, peptostreptococcus and coprococcus) were found in significantly higher frequency in crohn's disease (cd) than in ulcerative colitis (uc) and in other diseased control subjects and were virtually absent in apparently healthy subjects. when the posterior probability of having cd was calculated on the basis of these agglutination reactions, 64% of patients with cd and 34% of patients with uc but only 10% of ...19836673050
an international study of agglutinins to eubacterium, peptostreptococcus and coprococcus species in crohn's disease, ulcerative colitis and control subjects.the world-wide occurrence of agglutinating antibodies to four coccoid anaerobes belonging to eubacterium, peptostreptococcus and coprococcus spp. was investigated in 937 coded sera from patients suffering from crohn's disease, ulcerative colitis, various other diseases and from healthy controls. positive results were found in 59% of patients with crohn's disease, 29% of patients with ulcerative colitis, and 8% of both diseased and healthy control subjects. patients with crohn's disease of the co ...19836628839
phase variation of hyaluronidase-producing peptostreptococci associated with periodontal disease.peptostreptococci isolated from diseased periodontal pockets produced large amounts of extracellular hyaluronidase throughout their growth cycle. phase variants, obtained by re-streaking aged colonies of the wild types, had different colonial morphology and shorter generation times. these variants produced less hyaluronidase, and enzyme production occurred only during the early stationary phase.19836575993
[nonclostridial anaerobic soft tissue infections].fourteen cases of nonclostridial anaerobic infections of soft tissues caused by peptococci, peptostreptococci, bacteroids or their combinations with e. coli, proteus, and staphylococcus aureus were studied. they are characterized by a rapid spread of serous-suppurative inflammation and the causative agents in loose fibrous connective tissue of subcutaneous adipose tissue (cellutitis), surface fascia (fasciitis), or skeletal muscles (myositis). dissolution of intercellular structures of the conne ...19836860161
gardnerella vaginalis and anaerobic bacteria in genital disease.in a study of gardnerella vaginalis and anaerobic bacteria in non-specific vaginitis (nsv) and other genital disease 89 patients attending a genital medicine clinic had vaginal samples examined for conventional pathogens and for quantitative analysis of g vaginalis and aerobic and anaerobic bacterial flora. the overall incidence of g vaginalis was 20%; g vaginalis (mean concentration 7.0 log10/g of secretion) occurred predominantly in patients with nsv (57%) but also in sexual contacts of non-sp ...19836600955
perineal crohn's disease complicated by pyogenic liver abscess during metronidazole therapy.a patient with ileal and perineal crohn's disease developed a pyogenic liver abscess caused by streptococcus milleri. the perineal process had been treated with high-dose metronidazole for the preceding 6 mo, and culture of a perianal abscess 2 mo before admission revealed heavy growth of streptococcus milleri (which was misidentified as an anaerobic peptostreptococcus). this case illustrates the difficulty in correctly identifying streptococcus milleri, the most frequent isolate from pyogenic l ...19836852451
anaerobic bacteria associated with osteomyelitis in domestic animals.specimens of bony tissue or adjacent soft tissue from 19 animals with osteomyelitis were cultured aerobically and anaerobically. fourteen specimens (74%) yielded anaerobic bacteria in pure culture or mixed with aerobic or facultative anaerobic bacteria. the most predominant genus encountered was an obligate anaerobe, bacteroides. the most frequently isolated bacteroides species was bacteroides asaccharolyticus, which was isolated 5 times. the most frequently isolated anaerobe was peptostreptococ ...19836853311
death of an african elephant from probable toxemia attributed to chronic pulpitis.a 31-year-old captive male african elephant (loxodonta africana) of 5,000-kg body weight died suddenly in ventral recumbency. lesions seen at necropsy were bilateral purulent pulpitis and periodontitis of both tusks, serous atrophy of coronary groove fat, grammocephalus cholangitis, myocardial and skeletal lipofuscinosis, and scattered segmental necrosis in the pectoral muscles. nonhemolytic streptococci, corynebacterium sp, peptostreptococcus anaerobius, fusobacterium nucleatum, and bacteroides ...19836643242
[clinical experience with cefotiam in otorhinolaryngologic infections].clinical studies of cefotiam (ctm), a new cephalosporin derivative, in otorhinolaryngological field were performed, and the results were summarized as follows. ctm was intravenously injected to 31 cases of otorhinolaryngological infections in daily dose of 1--4 g. clinical efficacy was 33.3% in acute otitis media and chronic otitis media (acute exacerbation) (6 cases), 90% in acute tonsillitis (including peritonsillitis, peritonsillar abscess) (20 cases), 50% in acute pharyngitis (2 cases), 100% ...19836325742
on the occurrence of enoate reductase and 2-oxo-carboxylate reductase in clostridia and some observations on the amino acid fermentation by peptostreptococcus anaerobius.enoate reductase present in clostridium kluyveri and clostridium spec. la 1 could be detected in three strains of c. tyrobutyricum and ten clostridia belonging to the groups of proteolytic and saccharolytic or proteolytic species, respectively. in c. pasteurianum, c. butyricum and c. propionicum enoate reductase could not be found even after growth on (e)-2-butenoate. a 2-oxo-carboxylate reductase was present in rather low activities in the non-proteolytic clostridia which produce enoate reducta ...19836354130
cecal microflora of turkeys fed low or high fiber diets: enumeration, identification, and determination of cellulolytic activity.examination of cecal contents or bacterial cultures thereof from turkeys fed either a high fiber (hf) or low fiber (lf) ration indicated that direct microscopic counts of microbes were significantly higher in hf-fed than in lf-fed birds. there was no significant difference in mean colony counts between the two groups of turkeys. in both lf and hf-fed birds, 77% of the microbes were gram-positive rods, 14% gram-negative rods, and 9% gram-positive cocci. the predominant microorganism was eubacteri ...19836306633
[clinical study of cefroxadine in the field of obstetrics and gynecology].clinical study of cefroxadine (cxd), an orally active cephalosporin, for the treatment of infections in the field of obstetrics and gynecology was carried out and the following results were obtained. fifteen strains were isolated in present study. these isolates were mainly e. coli and anaerobes (peptococcus sp., peptostreptococcus sp.). the distribution of susceptibilities to cxd of e. coli was between 6.25 approximately 12.5 micrograms/ml and that of anaerobes was between 0.39 approximately 1. ...19836655870
the effect of lincomycin-neomycin treatment on experimental anaerobic bacterial bovine mastitis.three healthy lactating quarters of a friesland cow were each experimentally infected with a pure culture of a strain of either bacteroides fragilis, eubacterium lentum or a peptostreptococcus sp. respectively. the onset and progression to clinical mastitis was monitored 12 hourly by examination for clinical signs of inflammation, bacterial culture, somatic cell counts and with a strip cup. all infected quarters developed clinical mastitis within 24 hours. the 2 quarters infected with b. fragili ...19836668573
[use of cephalosporins as antibiotic prophylaxis in cesarean section].cefoxitin, a second-generation cephalosporin, was compared with cefazolin, a first-generation cephalosporin, and a placebo in a prospective, double-blind study of antibiotic prophylaxis in women undergoing nonelective cesarean section. in the groups that received cefazolin or the placebo there eas no statistically significant change in colonization of the cervix by aerobic bacteria by the fourth day after the operation, but there was a statistically significant increase in colonization by anaero ...19836344970
in vitro activity of u-57930e against anaerobic bacteria and its comparison with clindamycin, ampicillin, carbenicillin and tetracycline.the in vitro activity of u-57930e, a pipecolic acid amide of clindamycin, was compared with those of clindamycin, ampicillin, carbenicillin and tetracycline against 321 anaerobic clinical isolates. the mic (micrograms/ml) of u-57930e that inhibited 95% bacteroides fragilis, peptococcus prevotii, b. melaninogenicus and p. asaccharolyticus was 0.0625; 0.03125 for peptostreptococcus anaerobius, b. vulgatus, propionibacterium and peptococcus species. clindamycin, on the other hand, gave mic values o ...19836678915
the recovery of anaerobic bacteria from swabs in three transport systems. statistical evaluation by application of a variance component model.the recovery of six anaerobic species from swabs in three transport systems was quantitatively evaluated. the test strains were bacteroides fragilis, b. melaninogenicus ss. intermedius, fusobacterium necrophorum, peptostreptococcus anaerobius, eubacterium lentum, and clostridium ramosum. the transport systems were a charcoal-impregnated cotton swab in modified stuart's transport medium (ms), or a plain cotton swab in either a dry aerobic tube (da) or an aerobic agar tube (aa). swabs were charged ...19836346790
frequency of occurrence and antimicrobial susceptibility of bacterial isolates from the intestinal and female genital tracts.in the selection of empiric therapy for infections of the female genital tract and intraabdominal infections in humans, the requisite information includes (1) the frequency of isolation of bacterial species dominant in the normal gastrointestinal and female genital tracts and in intraabdominal and female genital tract infections and (2) the in vitro susceptibilities of the dominant species to drugs that may be used against them (e.g., penicillin g, cefoperazone, cefoxitin, chloramphenicol, clind ...19836342102
bacteriology of dental abscesses of endodontic origin.aspirates have been cultured from 10 dental abscesses of endodontic origin, all of which had penetrated beyond the bony alveolus to produce fluctuant swelling. sampling was by syringe aspiration. strict anaerobic techniques, including the use of an anaerobic chamber, were used for serial dilution and plating. randomly selected colonies (100) from each culture were purified, characterized, and identified. seventy percent of the bacterial isolates were either strict anaerobes or microaerophilic. o ...19836630460
[cefotiam passage into the maxillary sinus tissues].clinical study was made on cefotiam (ctm) and the following results were obtained. tissue concentrations of ctm were determined 1 hour after intravenous injection (ctm 1 g) in chronic sinusitis and maxillary cyst. concentrations of ctm were 16.53 micrograms/g, 13.26 micrograms/g in mucosa of the maxillary sinus, maxillary cyst, respectively. antibacterial activity of ctm was measured on escherichia coli, staphylococcus epidermidis, aerococcus, acidaminococcus fermentans, peptostreptococcus anaer ...19836325743
purification and characterization of nadp-dependent 7 beta-hydroxysteroid dehydrogenase from peptostreptococcus productus strain b-52.an nadp-dependent 7 beta-hydroxysteroid dehydrogenase was purified 11.5-fold over the activity in crude cell extracts prepared from peptostreptococcus productus strain b-52, by using sephadex g-200 and deae-cellulose column chromatography. 7 beta-dehydrogenation was the sole transformation of bile acids catalyzed by the partially purified enzyme. the enzyme preparation (spec. act. 2.781 iu per mg protein) had an optimum ph of 9.8. lineweaver-burk plots showed a michaelis constant (km) value of 0 ...19836572075
[bacterial flora isolated from the cervical secretions of patients with acute gonorrhea and their sensitivities to spectinomycin and ampicillin].subsequent to studies of clinical effects of spectinomycin (spcm) and ampicillin (abpc) given in combination on acute gonorrhea in female patients, sensitivity of n. gonorrhoeae to each of these antibiotics was determined. furthermore, cervical discharge of female patients was searched for other organisms. combined antimicrobial action of spcm and abpc were also examined. aerobic and anaerobic organisms were obtained from cervical discharge of 20 cases of acute gonorrhea. the predominant aerobe ...19836228677
[clinical evaluation of tinidazole on anaerobic infections in the field of obstetrics and gynecology].the bacteriological and clinical effect of tinidazole (tdz) was evaluated in 16 cases of intrauterine, intrapelvic and vulvar infection caused by anaerobic organisms and the following results were obtained. anaerobes were detected in 16 cases, including 1 case with anaerobes alone and 15 cases with mixed anaerobes and aerobes. eight different species and 24 strains were detected. a single species was isolated from 9 cases, 2 species from 6 cases and 3 species from 1 case. the main species detect ...19836674545
effect of glucose concentration in the growth medium upon neutral and acidic fermentation end-products of clostridium bifermentans, clostridium sporogenes and peptostreptococcus anaerobius.clostridium bifermentans, c. sporogenes and peptostreptococcus anaerobius were grown in fastidious anaerobe broth with glucose concentrations from 0-1.5% (w/v). gas chromatographic (glc) analysis of fermentation end-products revealed that a change in glucose concentration markedly affected fermentation patterns of each microorganism. increased glucose concentration resulted in stimulation of ethanol production by all strains, and of butanol and butyric acid by c. sporogenes. with c. bifermentans ...19836822993
anaerobic pyogenic sacroiliitis. 19836844970
effect of eight growth media upon fermentation profiles of ten anaerobic bacteria.a study was made of the influence of eight growth media upon the fermentation end-product patterns obtained with ten species of anaerobes. acidic and neutral end-products of fermentation were analysed by gas chromatography and the results were examined statistically by computer. differences in end-product profile were at least as great between different media used to grow a single anaerobic species, as between different anaerobes grown in a single type of medium. when individual fermentation end ...19836853401
aspiration and lung abscess in cystic fibrosis.large abscess cavities are not commonly encountered in patients with cystic fibrosis (cf). one such patient is reported in whom an abscess developed secondary to aspiration of sand and salt water. material obtained at bronchoscopy revealed anaerobes as well as the aerobic organisms previously cultured from her sputum. rapid improvement occurred after institution of antibiotic therapy specifically directed against the anaerobic organisms.19836859663
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