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in vitro evaluation of the effectiveness of calcium hydroxide and paramonochlorophenol on anaerobic bacteria from the root canal.the effectiveness of calcium hydroxide and paramonochlorophenol (pmcp) was tested on anaerobic bacteria isolated from infected root canals, at time intervals 5, 15, 30 and 60 min. the experimental method used was similar to that proposed by the german society of hygiene and microbiology (dghm), with slight modifications. testing revealed that calcium hydroxide was significantly more effective than pmcp against the total number of anaerobes. additionally, it was proved to be quickly and highly ef ...19938143576
[what kinds of species are in the conjunctiva?].with suitable culture methods less than 5% of conjunctival fluid specimens remain negative. from approximately 95% of the swab cultures at least 65 bacterial species - although in low numbers - were recovered. among the predominant organisms were gram-positive species like several coagulase-negative staphylococcus spp., propionibacterium spp., corynebacterium spp. and members of the genera peptostreptococcus, streptococcus and actinomyces. among the frequently isolated gram-negative species were ...19938144184
antimicrobial activity of dv-7751a, a new fluoroquinolone.we compared the in vitro antibacterial activity of dv-7751a against gram-positive and -negative bacteria with those of quinolones currently available. mics for 90% of the strains tested (mic90s) against clinical isolates of methicillin-susceptible and -resistant staphylococcus aureus and staphylococcus epidermidis were 0.20, 0.39, 0.20, and 0.78 micrograms/ml, respectively. moreover, mic50s for dv-7751a against ofloxacin-resistant methicillin-resistant s. aureus were 4-, 8-, 16-, 32-, and 64-fol ...19938257132
[the role anaerobes in dermatitis digitalis et interdigitalis in cattle].altogether 52 tissue samples and swabs from the corium of the claw of diseased cattle were investigated. microbiological examinations revealed the presence of 11 strictly anaerobic bacterial strains that belonged to the species peptococcus asacharolyticus, pc. sacharolyticus, peptostreptococcus anaerobius, bacteroides asacharolyticus and b. melaninogenicus. the remaining isolates were classified as bacteroides, fusobacterium, streptococcus and clostridium. in the above-mentioned strains observat ...19938259638
an in-vitro study of the comparative viability on different swab types of simulated specimens of bacteria commonly present in oro-facial infections.an in-vitro study was undertaken to investigate the survival on bacteriological swabs of three potentially pathogenic organisms found in oro-facial infections, by using simulated clinical specimens incubated in the presence or absence of amies transport medium. standard inocula of pure cultures of streptococcus milleri, prevotella melaninogenica and peptostreptococcus anaerobius were soaked on to swabs and plated out on to solid media at various intervals up to 3 days, enabling an estimate of th ...19938099810
microbiology of the pericoronal pouch in mandibular third molar pericoronitis.the microorganisms associated with mandibular third molar pericoronitis were investigated using direct microscopy and anaerobic culture method. the pericoronal pouch was sampled with paper points in a) 8 patients without mandibular third molar pericoronitis and b) 6 patients with mandibular third molar pericoronitis. under the microscope, the microflora was found to be a complex mixture comprising gram-positive and gram-negative cocci, rods and filaments (including fusiform and curved rods), mot ...19938265205
cytotoxicity versus antibacterial activity of some antiseptics in vitro.through the development of new techniques for bacterial growth and isolation, obligate anaerobes have been shown to be more prevalent than previously thought in the pathogenesis of periapical and pulpal diseases. a follow-up study was conducted to examine the germicidal effect of 0.05% naocl, 10 mg/ml metronidazole and supernatant of ca (oh)2 (0.025 ml) on four anaerobic microorganisms commonly found in root canals, and to compare the toxicity of these substances on cell cultures. in vitro testi ...19938100851
anaerobic bacteria in orofacial abscesses.obligate anaerobic bacteria were cultured from 15 orofacial abscesses. bacteroides species constituted 43 per cent, clostridium 21 per cent, fusobacterium 14 per cent, peptostreptococci 11 per cent, peptococci 7 per cent and veillonella 4 per cent of the isolates. this study confirms the polymicrobial nature of orofacial infections. sensitivity to antibiotics was unpredictable. clostridium difficile, clostridium tetani, peptostreptococcus productus and veillonella parvula showed resistance to so ...19939508939
bacteriology of anaerobic pleuropulmonary infections: preliminary report.a retrospective bacteriologic study of anaerobic pleuropulmonary infections diagnosed at the wadsworth veterans affairs medical center between 1976 and 1991 was performed. there were 116 specimens from 110 patients. available strains were reexamined using the latest tests and taxonomic schemes. pleural fluid was believed to provide the most reliable specimen; cultures yielded an average of 3.0 anaerobes and 0.6 nonanaerobes per specimen. the most commonly encountered anaerobes were pigmented pre ...19938324128
the influence of race and gender on periodontal microflora.previous studies have demonstrated that demographic characteristics of subject populations influence both the incidence of periodontal diseases and various aspects of host responses to periodontal bacteria. in this study we analyzed the components of the subgingival microflora from individuals with adult periodontitis, early onset periodontitis, gingivitis, and periodontal health as a function of gender and race (black and white). clinical categories were analyzed individually so that there were ...19938387107
the acute phase of experimental infection with trypanosoma cruzi is more severe in mice monoassociated with strict anaerobic bacteria.1. the influence of some components of the normal human intestinal flora on the acute phase of experimental infection with strain cl of trypanosoma cruzi was studied in 30-day-old germ-free or gnotobiotic cfw (lob) mice monoassociated with bacteroides fragilis, peptostreptococcus sp or clostridium sp by intragastric inoculation of 10(6) bacteria 10 days before the intraperitoneal infection with 5 x 10(3) trypomastigotes/g body weight. 2. significantly earlier parasitemia peak and mortality were ...19921342224
summer mastitis in heifers: studies on the seasonal occurrence of actinomyces pyogenes, peptostreptococcus indolicus and bacteroidaceae in clinically healthy cattle in denmark.with the aim of investigating the seasonal occurrence of actinomyces pyogenes, peptostreptococcus indolicus, bacteroides melaninogenicus ss. levii and fusobacterium necrophorum, and thus the potential for development of summer mastitis, clinically healthy danish holstein-friesian heifers due to calve in the autumn were sampled from the teat tip, the conjunctiva and the oral cavity at 2-6 week intervals from 1979 to 1981. the overall isolation rates of f. necrophorum, p. indolicus and b. melanino ...19921348381
periodontal bone loss in mice induced by different periodontopathic organisms.periodontal bone loss in mice orally inoculated with peptostreptococcus anaerobius, pept. magnus and actinobacillus actinomycetemcomitans was compared to that in sham-inoculated mice. six-to-8-week-old balb/c mice were inoculated with 1 x 10(5), 1 x 10(7) or 1 x 10(9) colony-forming units (c.f.u.) of bacteria in 50 microliters of medium. ten mice received each concentration of bacteria and 10 sham-inoculated mice acted as controls. five mice from each of the groups were killed 6 weeks after inoc ...19921353347
immunoglobulins in milk from cows immunized with oral strains of actinomyces, prevotella, porphyromonas, and fusobacterium.immunization of pregnant cows with bacteria leads to the presence of high concentrations of specific antibodies in colostrum and milk. a total of 14 cows was immunized with single strains of heat-killed oral bacteria or pools of strains of actinomyces, porphyromonas, prevotella, and fusobacterium. two cows were treated with adjuvant alone. the mean percentages of igg1, igg2, igm, and iga in all of the milks were 83.8, 3.8, 9.3, and 3.1, respectively. elisa and whole cell agglutination assays dem ...19921354674
comparative bacteriological studies on summer mastitis in grazing cattle and pyogenes mastitis in stabled cattle in denmark.a total of 143 secretions from clinical cases of summer mastitis (sm) in grazing cattle and from 89 cases of pyogenes mastitis (pm) in stabled cattle were examined bacteriologically. the typical bacteriological finding was a mixed flora in which the predominant organisms were actinomyces pyogenes (sm-70%, pm-85%), peptostreptococcus indolicus (54%, 54%), a microaerophilic coccus (stuart-schwan coccus) (26%, 25%), fusobacterium necrophorum biovar b (22%, 12%), bacteroides melaninogenicus (20%, 9% ...19921355316
heterogeneity of surfaces of subgingival bacteria as detected by zeta potential measurements.porphyromonas gingivalis, prevotella intermedia, and actinobacillus actinomycetemcomitans (a.a.) are gram-negative bacteria which are implicated in various forms of periodontal disease. the gram-positive peptostreptococcus micros may also play an important role. for investigation of the possible adhesion and colonization mechanisms of these organisms, the charge properties of the outermost layers of bacterial cell surfaces were studied through the measurement of zeta potentials at various ph val ...19921357016
the use of dna probes to examine the distribution of subgingival species in subjects with different levels of periodontal destruction.the present investigation examined the distribution of 14 subgingival species at a total of 2299 sites in 90 subjects with different levels of periodontal destruction. subgingival plaque samples taken from the mesial aspect of each tooth were anaerobically dispersed, diluted and plated on non selective media. after anaerobic incubation, colonies were lifted to nylon filters and specific species detected using digoxygenin-labeled whole chromosomal dna probes. the mean total viable count for all s ...19921318331
subgingival temperature (iii). relation to microbial counts.the present investigation examined the relationship of selected bacterial species and subgingival temperature. 35 subjects were measured at 6 sites per tooth for clinical parameters and subgingival temperature. measurements were repeated for 21 subjects at 2 month intervals providing a total of 66 subjects visits. at each visit, subgingival plaque samples were taken from the mesial aspect of each tooth and anaerobically dispersed, diluted and plated on non-selective media. after anaerobic incuba ...19921321846
e-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms.the purpose of the study was to validate the epsilometer test (e-test) method for antimicrobial susceptibility testing of selected periodontopathic microorganisms using the agar dilution method as a standard. the e-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms. the device consists of a predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip. after 48 hours incub ...19921324301
three new species of the genus peptostreptococcus isolated from humans: peptostreptococcus vaginalis sp. nov., peptostreptococcus lacrimalis sp. nov., and peptostreptococcus lactolyticus sp. nov.we describe three new species of the genus peptostreptococcus which were isolated from human specimens and were tentatively identified as peptostreptococcus prevotii. these three organisms were not homologous with previously described type strains of the genus peptostreptococcus. a total of 12 strains that were identified biochemically as p. prevotii were divided into five independent dna similarity groups; 10 of these strains were divided into three similarity groups which exhibited significant ...19921390111
microbiology of secondarily infected diaper dermatitis.specimens obtained from 67 infants with secondarily infected diaper dermatitis were cultured for aerobic and anaerobic bacteria. bacteria growth was obtained in 58. aerobic facultative bacteria or candida sp. only were present in 28 patients (48%), anaerobic bacteria only in 11 (19%), and mixed anaerobic with aerobic, facultative, or yeast flora was present in 19 (33%). ninety-one bacterial or fungal isolates were recovered (1.6 per specimen), 54 (0.9 per specimen) aerobic or facultative bacteri ...19921399196
anaerobic bacterial infections causing osteomyelitis/arthritis in a dog.a 3-year-old german shepherd dog was examined for lameness, signs of pain, swelling, a draining fistulous tract, and osteolysis after a dog bite on the left carpus. after failure of the lesion to respond to several antibiotics, peptostreptococcus sp and propionibacterium sp were isolated from swab specimens and then from surgically collected bone and soft tissue specimens. the bone fragments had mild purulent osteomyelitis associated with numerous gram-positive rods and cocci. the dog was succes ...19921399798
enzymatically active peptostreptococcus magnus: association with site of infection.fifty-four strains of peptostreptococcus magnus (11 were recovered from abdominal infections, 18 were from nonpuerperal breast abscesses, and 21 were from diabetic foot infections; the type strain and three other strains were from the american type culture collection, rockville, md.) and the type strain of peptostreptococcus micros were tested for their ability to produce various enzymes, including catalase, hippurate hydrolase, serine dehydratase, threonine dehydratase, collagenase, gelatinase, ...19921400997
necrotizing fasciitis of the neck and chest. report of a case.a case is presented of necrotizing fasciitis of the neck and chest characterized by rapid progressive necrosis of subcutaneous tissue, fascia and skin. the diagnosis and management is discussed.19921402054
[asporogenic anaerobic thoracic empyema--an experimental model for anaerobic thoracic empyema in rabbits].sterile pleural effusion was induced by intrapleural administration of turpentine, and empyema was induced by direct intrapleural inoculation of bacteria in rabbits. experimental models of anaerobic thoracic empyema were successfully made in this study, using escherichia coli, peptostreptococcus asaccharolyticus, bacteroides fragilis, using the following methods; (1) a single bacterium inoculation of, a) e. coli, b) p. asaccharolyticus, c) b. fragilis, (2) simultaneous inoculations with an aerob ...19921402083
experimentally induced infection by oral anaerobic microorganisms in a mouse model.a mouse model was developed to study the abscessogenic potential of pure and mixed cultures of oral anaerobes associated with infections of endodontic origin. maximum subcutaneous lesions were seen on their backs between 3 and 14 d after inoculation. strains of fusobacterium nucleatum, peptostreptococcus anaerobius, and veillonella parvula were pathogenic in pure culture. a mixed culture of f. nucleatum with either porphyromonas gingivalis or prevotella intermedia was significantly more pathogen ...19921408362
anaerobic microflora associated with the pars oesophagea of the pig.in healthy pigs aged one to 35 days significant populations of microorganisms (5.5 to 6.9 log10 viable count cm-2 of the tissue) were found, by strict anaerobic techniques, to adhere to the pars oesophagea. the genera of anaerobes which were isolated included actinomyces, bifidobacterium, clostridium, lactobacillus, peptostreptococcus, streptococcus and veillonella and facultative anaerobes included escherichia, klebsiella, staphylococcus, streptococcus and yeasts. the microbial population adher ...19921410807
the relative lethality of intestinal bacteria for gnotobiotic rats with experimental intestinal strangulation.in eight experiments utilizing 28 animals each, germfree rats with and without ischemically or hemorrhagically strangulated closed loop intestinal segments were contaminated with either one of four common intestinal anaerobic bacteria, or a combination of two or three bacteria previously found to be innocuous in pure culture. the results showed that: a) in pure culture, bacteroides fragilis, bacteroides melaninogenicus, and peptostreptococcus anaerobius were innocuous; b) fusobacterium necrophor ...19921479304
the endodontic microflora revisited.the microbial flora of 35 dental root canals were examined, taking care to maintain the viability of obligate anaerobes which accounted for 45% of total isolations, while streptococcal species accounted for 24% of the total species isolated. individual root canals yielded a maximum of eight bacterial species. a total of 40 different species was isolated of which the most prevalent were the facultative anaerobe streptococcus sanguis and the obligate anaerobe, peptostreptococcus micros (both in 23 ...19921479923
susceptibility of anaerobic microorganisms to hypothiocyanite produced by lactoperoxidase.the susceptibility of capnocytophaga ochracea, eikenella corrodens, eubacterium yurii, fusobacterium nucleatum, peptostreptococcus micros, prevotella intermedia, selenomonas sputigena, wolinella recta to hypothiocyanite (oscn-) produced by the lactoperoxidase system was tested. results showed a decrease of bacterial survival rate after oscn- exposure, with an intra- and inter-species variability from 0 to 95% for c. ochracea, 34-100% for e. corrodens, 0-83% for e. yurii, 1-15% for f. nucleatum, ...19921481764
comparative in vitro activity of ro 40-6890, ro 41-3399, and other antimicrobial agents against anaerobic bacteria.the in vitro activity of the ester ro 41-3399 and its free active acid ro 40-6890 was tested against 189 strains of anaerobic bacteria in comparison to other oral cephalosporins and to antimicrobial agents established in the treatment of anaerobic infections. prevotella, porphyromonas, peptostreptococcus, fusobacterium and clostridium spp. were susceptible to ro 40-6890, with few exceptions. due to its lack of activity against the major pathogens of the bacteriodes fragilis group, ro 40-6890 doe ...19921486894
[pharmacokinetic, bacteriological, and clinical studies on panipenem/betamipron in children].pharmacokinetic, bacteriological and clinical studies were performed on panipenem/betamipron (papm/bp) in children. the results are summarized as follow: 1. twelve patients with various bacterial infectious diseases were treated with papm/bp. each dose was 20 mg/20 mg/kg, administered 3 times daily, in 30-minute intravenous drip infusion. treatments were continued for 5-22 days. clinical efficacies of papm/bp in 12 patients with bacterial infections (1 with suspected sepsis, 5 with pneumonia, 1 ...19921512926
[antibacterial activity of rokitamycin against fresh clinical isolates].we obtained bacterial strains which were clinically isolated and identified from outpatients with various infections in medical institutions throughout japan. possible antibacterial activities of rokitamycin (rkm) were examined against these isolates. minimum inhibitory concentrations (mics) were determined through a comparative study with reference drugs. the results of the study are summarized as follows. 1. resistance patterns of 400 isolates which were highly resistant to macrolides (mls) wi ...19921289579
[antimicrobial activities of ceftriaxone against clinically isolated strains].antibiotic activities (mics) of ceftriaxone (ctrx) against 1,210 strains of bacteria including 28 spp. isolated in 1987 and 1990 were compared with those of other cephems. 1. when compared to data on clinically isolated strains reported in the early 1980s, strains of the following species isolated in 1990 showed extremely elevated mic90s of ctrx: staphylococcus spp., streptococcus pneumoniae, escherichia coli, citrobacter spp., enterobacter spp., serratia spp., proteus vulgaris, morganella morga ...19921522669
resistance of anaerobic bacteria to antimicrobial agents in spain.as a consequence of antibiotic consumption, the entire microbial ecosystem attached to man is evolving towards resistance. in spain, penicillin resistance (mic > 0.5 mg/l) is found in about 10% of peptostreptococcus, clostridium perfringens and eubacterium, and in 50% of veillonella. cefoxitin resistance ( > 2 mg/l) is present in 10 to 20% of peptostreptococcus and clostridium, and in 50% of eubacterium; 21% of bacteroides (fragilis group) strains are resistant to 16 mg/l. a very low rate of imi ...19921295754
enumeration of subgingival species on primary isolation plates using colony lifts.this study evaluated the feasibility of using a colony lift method and dna probes to enumerate bacterial species cultured on primary isolation plates. fourteen digoxigenin-labeled whole chromosomal dna probes representing 12 subgingival species were validated by hybridization with colony lifts prepared from 249 reference strains of 51 species grown on trypticase soy agar plates supplemented with 5% sheep blood. colonies of reference strains were lifted onto nytran filters from plates and treated ...19921528619
the relationship between clinical symptoms and anaerobic bacteria from infected root canals.the purpose of this study was to investigate the correlation between the composition of bacterial flora from infected root canals and clinical symptoms. the materials evaluated consisted of 28 teeth from 25 patients with apical periodontitis. eubacterium were found to be significantly related to acute or chronic clinical symptoms and peptococcus, peptostreptococcus, and porphyromonas gingivalis to subacute clinical symptoms. we suggested that peptococcus, peptostreptococcus, eubacterium, porphyr ...19921298793
peptostreptococcus micros in human periodontitis.peptostreptococcus micros is a recognized pathogen in medical infections, and its association with progressive periodontitis was examined in this study. p. micros was isolated from paper-point subgingival samples on anaerobic enriched blood agar plates and identified on the basis of cellular and colonial morphology and selected biochemical tests. in a cross-sectional study involving 907 people with advanced adult periodontitis, 127 with early-onset periodontitis, and 12 with localized juvenile p ...19921528618
infection and labor. vii. microbial invasion of the amniotic cavity in spontaneous rupture of membranes at term.the purpose of this study was to determine the frequency, microbiologic characteristics, and clinical significance of microbial invasion of the amniotic cavity in women with premature rupture of membranes at term.19921301006
diagnosis and management of parotitis.of all the salivary glands, the parotid gland is most commonly affected by an inflammatory process. infections of the parotid gland range from acute to severe. assessment of the disease process should differentiate local primary parotid infection from systemic infection when this gland is also involved as part of a generalized inflammatory condition. viral parotitis can be caused by paramyxovirus (mumps), epstein-barr virus, coxsackievirus, and influenza a and parainfluenza viruses. acute suppur ...19921571113
in vitro activities of three of the newer quinolones against anaerobic bacteria.the antimicrobial activities of three new quinolone compounds, sparfloxacin, temafloxacin, and win 57273, against anaerobic bacteria were determined in three separate studies. the wadsworth agar dilution technique using brucella-laked blood agar was used throughout. the activities of other antimicrobial agents, including ciprofloxacin, imipenem, chloramphenicol, metronidazole, cefotetan, cefoxitin, and amoxicillin-clavulanic acid, were also determined. the breakpoints of the new quinolones were ...19921317149
antimicrobial activity of du-6859, a new potent fluoroquinolone, against clinical isolates.du-6859, (-)-7-[(7s)-amino-5-azaspiro(2,4)heptan-5-yl]-8-chloro-6- fluoro-1-[(1r,2r)-cis-2-fluoro-1-cyclopropyl]-1,4-dihydro-4-oxoquinol one-3- carboxylic acid, is a new fluoroquinolone with antibacterial activity which is significantly better than those of currently available quinolones. the mics for 90% of methicillin-susceptible and -resistant staphylococcus aureus and staphylococcus epidermidis clinical isolates (mic90s) were 0.1, 3.13, 0.1, and 0.39 microgram/ml, respectively. mic50s of du- ...19921324647
comparative in-vitro activity of azithromycin, macrolides (erythromycin, clarithromycin and spiramycin) and streptogramin rp 59500 against oral organisms.the in-vitro activities of azithromycin, clarithromycin, spiramycin and rp 59500 were compared with erythromycin against a wide range of oral organisms which have been implicated in oral infections and/or endocarditis (clindamycin was included for oral streptococci). all compounds tested showed good activity against many of these organisms, although some variation was observed with different species. clarithromycin was the most active of the antibiotics tested against gram-positive anaerobes, in ...19921331019
antibacterial efficacy of root canal medicaments.the antibacterial efficacy of four endodontic medicaments was studied in vitro. calcium hydroxide (calacept), camphorated parachlorophenol (cpcp), cresophene, and 2% iodine-potassium iodide (iki) were tested against four anaerobic microorganisms, streptococcus mutans, peptostreptococcus anaerobius, porphyromonus gingivalis and fusobacterium nucleatum, for 10 or 15 min. iki was effective only against f. nucleatum and p. gingivalis, and the others were effective against all four microorganisms.19921337555
physicochemical and structural investigation of the surfaces of some anaerobic subgingival bacteria.the surfaces of nine clinical isolates of porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, and peptostreptococcus micros and that of laboratory strain p. gingivalis w83 were studied by using contact angle measurements, x-ray photoelectron spectroscopy, infrared spectroscopy, microelectrophoresis of whole cells, and transmission electron microscopy of whole and sectioned cells. p. intermedia strains were hydrophilic, as judged from their small water contact a ...19921599251
contrast-enhanced magnetic resonance imaging of sub- and epidural empyemas.contrast-enhanced magnetic resonance images (mri) of three patients with subdural (sde) and two with epidural empyemas (ede) were reviewed. in each case, the capsule of the lesion demonstrated enhancement, and distinction between capsule and contents was obvious on contrast-enhanced images. in sde, contrast-enhanced images clearly depicted thickening of the neighbouring dura mater and a co-existent brain abscess. in ede, part of the displaced dura mater did not enhance, which facilitated differe ...19921359449
modulation of human neutrophil adherence by oral bacteria.polymorphonuclear neutrophils (pmns) comprise over 90 per cent of leukocytes in the oral cavity. although these phagocytic cells have primary defence roles in the gingiva, their stimulation by micro-organisms may also cause substantial tissue damage due to the release of lysosomal enzymes and oxygen radicals. adherence of pmns to the endothelium and their subsequent diapedesis and egress to areas of infection are considered early vital events in the inflammatory process. in this study, oral bact ...19921605750
prosthetic valve endocarditis and paravalvular abscess caused by peptostreptococcus magnus. 19921617069
microbiologic and pathologic aspects of pulpal and periapical disease.the greatest cause of endodontic and periapical pathosis is microbial infection of the pulp. most odontogenic infections are of a polymicrobial nature. with advances in anaerobic isolation and culturing techniques, much has been learned about the presence of pathogenic organisms such as porphyromonas and prevotella species (formerly classified as black-pigmented bacteroides species) in infected root canals. this review provides a summary of recent developments in endodontic microbiology, virulen ...19921381632
isolation and identification of intestinal bacteria from japanese tree frog (hlya japonica) with the special reference to anaerobic bacteria.the bacteria in the large intestines of eight japanese tree frogs (hlya japonica) were enumerated by using an anaerobic culture system. the microorganisms at approximately 3.1 x 10(9) bacteria per g (wet weight) of intestinal contents were present in the intestine of all the frogs tested. no difference of the total bacteria in the frog intestine was observed between two different incubation-temperatures (room temperature and 37 degrees c). eleven genera and 16 species were isolated from the frog ...19921391180
impact of the advances in age on the gastrointestinal microflora of beagle dogs.the gastrointestinal microflora of male beagle dogs in two different age groups; i) less than month 12 of age, and ii) more than year 11 of age, was compared. no detectable difference occurred on the microflora of stomach, duodenum, jejunum, and ileum in both dogs. large bowel (cecum, colon and rectum) microflora in both dogs yielded the different microbial populations. in all regions of large bowel, the levels of bacteroides, eubacteria, peptostreptococci, bifidobacteria, lactobacilli, and stap ...19921391181
bacteriology of periodontal disease.the microbial flora associated with the periodontal tissues in health and disease is extremely complex, and much research is being directed toward identifying those species that may be etiologic agents or that can be used as prognostic indicators. recent work has resulted in changes in the taxonomic position of several periodontal species and the recognition that several others, particularly species of eubacterium and peptostreptococcus, as well as a novel oral spirochete, may be important in di ...19921392003
[an increased incidence of bacterial endocarditis in chronic inflammatory bowel diseases].of 92 consecutive patients treated for proven native valve endocarditis three had ulcerative colitis and 2 crohn's disease. all 5 patients developed severe complications; three had to undergo emergency valve replacement. with a prevalence of 64.1/10(5) cases of inflammatory bowel disease the calculated incidence (5/92) revealed a significant over-representation of inflammatory bowel disease among patients with proven endocarditis (p less than 5.08 x 10(-9)). possible explanations may be the supp ...19921636271
bacterial invasion of non-exposed dental pulp.anaerobic procedures were adopted to demonstrate the early bacterial invasion of non-exposed dental pulps, and to isolate and identify the bacteria. of 19 freshly extracted teeth which originally exhibited deep dentinal lesions, clinical examination and electric pulp testing showed that nine of them had no pulpal exposure. thus the pulps of these teeth were covered by clinically sound dentine beneath the carious lesion. bacteria were found to have invaded the pulps of six of these nine teeth. th ...19921399049
[bacteriology of infectious disease in otorhinolaryngology (1). bacteriological study of paranasal cyst].the bacteriology of chronic sinusitis has been reported in several studies, but there have been few bacteriological studies on paranasal cyst. also, details of the techniques of transportation and processing of clinical specimens have only rarely been described. therefore, this study was undertaken with attention to the above, and to obtain information on appropriate antimicrobial therapy, we conducted a dilution antimicrobial susceptibility test of isolates. fifteen patients with paranasal cyst ...19921403316
[experience with the management of endometritis in the instituto nacional de perinatalogía].endometritis is the most frequent infectious complication of the puerperal period; with the objective to know the clinical characteristics, etiology and evolution of this disease we did a retrospective study of the endometritis cases among the patients of the instituto nacional de perinatología (inper) from january 1st, 1990 to may 31, 1991. during the revision period were diagnosed 120 cases, but we were able to examine only 90. in the 90% the resolution of the pregnancy was by means of caesare ...19921427285
abnormal intracerebral thallium localization in a bacterial brain abscess.a 56-yr-old man with a prior history of renal cell carcinoma had moderately intense abnormal localization of 201tl-chloride in a solitary brain lesion which was discovered to be a bacterial abscess. the organisms isolated by culture included actinomycosis odontolyticus, peptostreptococcus and hemophilus aphrophilus. because of the clinical presentation, mri characteristics and thallium scintigraphic findings, the lesion had been felt to represent either a primary or a metastatic neoplasm. this c ...19921432164
prevention of malodor from intraoral gauze tamponade with the topical use of clindamycin.azulene ointment with a small dose of clindamycin was used topically in eight patients with maxillary cancer with the intent to inhibit the malodor that originates from a gauze tamponade applied to the postoperative maxillary bony defect. the malodor was either markedly decreased or eliminated in all cases. concurrent with the decrease or elimination of the malodor, anaerobic microorganisms such as bacteroides and peptostreptococcus, which are considered to be involved in the generation of malod ...19921508511
changes in the microbial flora of airline headset devices after their use.the bacterial flora of 20 headset devices were evaluated before and after they were worn for 1 hour. bacteria were recovered from all headsets, and their number increased from a mean (+/- standard deviation) of 60 +/- 5 organisms per device to 650 +/- 51 organisms per device (p less than .001). the predominant organisms recovered were staphylococcus spp, streptococcus spp, propionibacterium spp, and peptostreptococcus spp. this study demonstrates the presence of potential pathogens in headset de ...19921731163
comparative evaluation of the metabolic potentials of different strains of peptostreptococcus productus: utilization and transformation of aromatic compounds.three strains of peptostreptococcus productus were tested for growth at the expense of methoxylated aromatic compounds. strain m8a-18 (human fecal isolate) was unable to utilize methoxylated aromatic compounds. while the type strain atcc 27340 (human septicemia isolate) was capable of minimal growth with methoxylated aromatic compounds, atcc 35244 (sewage sludge isolate) displayed significant growth on methoxylated aromatic compounds. methoxylated phenols, benzoates, benzyl alcohol and phenylacr ...19921521774
multicentre study on antibiotic susceptibilities of anaerobic bacteria to cefoperazone-sulbactam and other antimicrobial agents.the antibiotic susceptibilities of 241 anaerobic bacteria recovered from six geographic sites in north america were tested by agar dilution to cefoperazone-sulbactam and other drugs. of the 189 bacteroides fragilis group isolates, only one was resistant to cefoperazone-sulbactam (0.5%) or ampicillin-sulbactam (0.5%), and none was resistant to ticarcillin-clavulanate or chloramphenicol. no resistance to cefoperazone-sulbactam was observed among the other bacteroides spp., clostridium spp., or pep ...19921737725
serum igg reactive with oral anaerobic microorganisms associated with infections of endodontic origin.numerous species of bacteria have been implicated with infections of endodontic origin. the purpose of this study was to compare the levels of serum igg antibodies reactive with a panel of 10 oral anaerobic microorganisms implicated in infections of endodontic origin. serum samples were collected from 4 patient groups that included healthy patients without endodontic or periodontal disease, patients with chronic adult periodontal disease, patients with endodontic disease and patients with combin ...19921528630
predominant obligate anaerobes in human periodontal pockets.this study was carried out to investigate the predominant anaerobic bacteria of periodontal pockets in patients with advanced periodontitis, who had no previous treatment other than supragingival scaling, no history of recent or chronic systemic illness, nor any intake of antibiotics within 6 weeks prior to bacteriological sampling. care was taken not to ignore tiny-colony-forming anaerobes, by means of a stereoscope and an anaerobic glove box system. out of 422 (100%) isolates, 380 (90%) were o ...19921531505
in vitro activities of three semisynthetic amide derivatives of teicoplanin, mdl 62208, mdl 62211, and mdl 62873.mdl 62208, mdl 62211, and mdl 62873 are three semisynthetic amide derivatives of teicoplanin (mdl 62208 is an amide of teicoplanin aglycone, mdl 62211 is an amide of the teicoplanin a2 complex, and mdl 62873 is the corresponding derivative of peak a2-2 of the complex). the three semisynthetic glycopeptides were evaluated for in vitro antibacterial activity in comparison with the parent drug (teicoplanin) and vancomycin. a variety of gram-positive bacteria of clinical origin, whose species were c ...19921534977
antibiotic susceptibility of anaerobic bacteria isolated in johannesburg.in vitro susceptibilities of 198 anaerobic bacteria to seven antibiotics were evaluated by the agar dilution method. in addition to testing amoxycillin/clavulanic acid in a 2:1 ratio against the bacteria, the combination was also tested against 63 isolates using fixed concentrations of clavulanic acid and serial dilutions of amoxycillin. penicillin and cefoxitin were not effective against beta-lactamase-producing bacteroides isolates and only 50% of isolates were susceptible to the 2:1 amoxycill ...19921448710
aerobic and anaerobic microbiology of external otitis.microbiological and clinical data from 46 patients with external otitis were retrospectively evaluated. specimens were processed for isolation of aerobic and anaerobic bacteria. a total of 42 aerobic bacteria, 22 anaerobic bacteria, and 3 candida albicans organisms were recovered. aerobic bacteria only were isolated from 31 patients (67%), anaerobic bacteria only were isolated from 8 (17%), and mixed aerobic and anaerobic bacteria were isolated from 4 (9%). c. albicans alone was recovered from t ...19921457667
protein lg: a hybrid molecule with unique immunoglobulin binding properties.immunoglobulin (ig)-binding bacterial proteins have attracted theoretical interest for their role in molecular host-parasite interactions, and they are widely used as tools in immunology, biochemistry, medicine, and biotechnology. protein l of the anaerobic bacterial species peptostreptococcus magnus binds ig light chains, whereas streptococcal protein g has affinity for the constant (fc) region of igg. in this report, ig binding parts of protein l and protein g were combined to form a hybrid mo ...19921460053
prospective study of blood culture during transesophageal echocardiography.to ascertain the incidence and significance of bacteremia associated with transesophageal echocardiography (tee), 132 consecutive patients (aged 17 to 73 years) free of apparent infection who were undergoing 135 transesophageal echocardiographic procedures from october 1990 to august 1991, were prospectively studied. for each procedure, two sets of blood cultures were obtained for culture 30 to 60 minutes before tee, immediately after, and 180 to 240 minutes after the procedure. for each blood c ...19921462911
experimental studies of survival of anaerobic bacteria at 4 degrees c and 22 degrees c in two different transport systems.the survival of anaerobic bacteria on swabs in two different transport systems at 4 degrees c and 22 degrees c was evaluated. the transport systems were a charcoal-impregnated cotton swab in modified stuart transport medium (mst), and a viscose swab in modified siff transport medium (btm) (bionor). the following eight clinical strains of anaerobic bacteria were tested for quantitative recovery at 24 h, 48 h, 72 h, and 96 h; fusobacterium necrophorum, bacteroides melaninogenicus, bacteroides inte ...19921472363
physico-chemical and structural properties of the surfaces of peptostreptococcus micros and streptococcus mitis as compared to those of mutans streptococci, streptococcus sanguis and streptococcus salivarius.the surface properties of nine streptococcus mitis and four peptostreptococcus micros strains from the oral cavity were examined and compared with a large group of oral streptococci. zeta potential and contact angle measurements were employed to determine physico-chemical cell surface properties. in addition, elemental surface concentration ratios were obtained via x-ray photoelectron spectroscopy, and surface structures were examined with transmission electron microscopy. the s. mitis and p. mi ...19921487734
the iron-sulfur-cluster-containing l-serine dehydratase from peptostreptococcus asaccharolyticus. stereochemistry of the deamination of l-threonine.the stereochemistry of the deamination of l-threonine to 2-oxobutyrate, catalyzed by purified l-serine dehydratase of peptostreptococcus asaccharolyticus, was elucidated. for this purpose the enzyme reaction was carried out with unlabelled l-threonine in 2h2o and in 3hoh, as well as with l-[3-3h]threonine in unlabelled water. isotopically labelled 2-oxobutyrate thus formed was directly reduced in a coupled reaction with l- or d-lactate dehydrogenase and nadh. the (2r)- or (2s)-2-hydroxybutyrate ...19921572371
the effect of amoxycillin on vaginal colonization resistance and normal vaginal flora in monkeys.previous studies have shown that vaginal colonization resistance in monkeys can be eliminated by amoxycillin and restored by flushing vaginal flora from a healthy monkey into the vagina of a monkey colonized with escherichia coli. the hypothesis that the effect of amoxycillin resulted from elimination of parts of the normal flora was tested in the present study. nine monkeys were flushed vaginally with amoxycillin daily for six days. the number of anaerobic bacteria decreased during amoxycillin ...19921592701
resistance of peptostreptococcus spp. to macrolides and lincosamides: inducible and constitutive phenotypes.the activities of erythromycin and clindamycin against 350 peptostreptococcus strains were studied during a 5-year period (1986 to 1991). in 5.1% of the peptostreptococcus strains, which presented dissociated resistance (clindamycin mic, less than or equal to 1 microgram/ml; erythromycin mic, greater than 8 micrograms/ml), evidence of inducible macrolide-lincosamide resistance was shown. a total of 17.7% of the strains presented a constitutive phenotype; the clindamycin and erythromycin mics for ...19921622181
susceptibilities of anaerobic bacteria isolated from animals with ovine foot rot to 28 antimicrobial agents.the agar dilution method was used to determine the inhibitory activities of 28 antimicrobial agents against 35 strains of the genus peptostreptococcus, 4 strains of the species peptococcus niger, 20 strains of the species megasphaera elsdenii, 7 strains from the species acidaminococcus fermentans, 8 strains of the genus clostridium, 11 strains of the genus eubacterium, and 1 strain of the species propionibacterium acidipropionici, all of which were isolated from 125 clinical cases of ovine foot ...19921590689
necrotizing fasciitis of the vulva.we attempted to characterize the natural history of necrotizing fasciitis of the vulva.19921595786
[gynecologic and obstetric infections caused by aerobic bacteria].the anaerobic bacteria (ab) are between the most numerous microorganisms (mo) that constitute the flora of the female genital tract, so they can participate in the etiology of obstetric and gynecologic infections (ogi). the objective of this study was to investigate the frequency of ab isolations and the clinical characteristics of the anaerobic infections (ai) in patients of the national institute of perinatology, from january 1st, 1988 to may 31, 1991. ab were isolated from 117 patients who de ...19921618410
[pyogenic liver abscess. its exclusive treatment with antibiotics]. 19921626100
spontaneous contrast echoes in pericardial effusion: sign of gas-producing infection. 19921636601
associations between microbial species in dental root canal infections.the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ...19921494447
spontaneous gas-forming bacterial myonecrosis caused by group b streptococci and peptostreptococci.we present the case of a patient who spontaneously developed a severe gas-forming infection involving the voluntary muscles of both thighs. the organisms responsible were group b beta-haemolytic streptococci together with peptostreptococci. following surgical and medical treatment, the patient fully recovered. no predisposing factors, in particular no disease causing immunocompromise, could be identified.19921600357
structure of peptostreptococcal protein l and identification of a repeated immunoglobulin light chain-binding domain.the gene for protein l, an immunoglobulin (ig) light chain-binding protein expressed by some strains of the anaerobic bacterial species peptostreptococcus magnus, was cloned and sequenced. the gene translates into a protein of 719 amino acid residues. following a signal sequence of 18 amino acids and a nh2-terminal region ("a") of 79 residues, the molecule contains five homologous "b" repeats of 72-76 amino acids each. further, toward the cooh terminus, two additional repeats ("c") were found. t ...19921618782
diagnosis and management of anaerobic infections of the head and neck.anaerobic bacteria are important pathogens in head and neck infections such as chronic otitis media, chronic sinusitis, chronic mastoiditis, head and neck abscesses, cervical adenitis, parotitis, and postoperative infection. bacteroides sp (bacteroides melaninogenicus group, bacteroides oralis, and bacteroides fragilis group), peptostreptococcus sp, and fusobacterium sp predominate. the observed recent increase in the number of beta-lactamase-producing strains of bacteroides sp isolated in head ...19921728904
protein l from peptostreptococcus magnus binds to the kappa light chain variable domain.protein l is an immunoglobulin light chain-binding protein expressed by some strains of the anaerobic bacterial species peptostreptococcus magnus. the major variable region subgroups of human kappa and lambda light chains were tested for protein l binding; v kappa i, v kappa iii, and v kappa iv bound protein l, whereas no binding occurred with proteins of the v kappa ii subgroup or with any lambda light chain subgroups. studies of the protein l binding capacity of naturally occurring vl fragment ...19921733930
the relationship of hydrogen peroxide-producing lactobacilli to bacterial vaginosis and genital microflora in pregnant women.lactobacilli provide an important microbial defense against genital colonization by pathogens. the role of hydrogen peroxide (h2o2) in the control of genital microflora was explored in a cross-sectional study of 275 women in the second trimester of pregnancy. vaginal cultures were obtained for detection of h2o2-positive and h2o2-negative lactobacilli and other members of the genital microflora. compared with women with h2o2-negative lactobacilli, women colonized by h2o2-positive lactobacilli wer ...19921738516
immunobiological activities of bacteria isolated from the root canals of postendodontic teeth with persistent periapical lesions.although intraradicular bacteria are widely considered to be the primary etiological agents of periapical lesions, the immunobiological properties of the species in question are not adequately known. the aim of this study, therefore, was to investigate the pathobiological properties of the 10 most frequently isolated endodontopathic bacterial species. using cellular components of the pathogens obtained by sonic extraction, we have investigated their ability to induce monocyte migration, interleu ...199219186419
[antibacterial activities of ofloxacin against recent isolates from patients with community-acquired infections].in order to survey antibacterial activities of ofloxacin (oflx) against 1,440 bacterial strains isolated from patients with community-acquired infections in 1987 and 1990, minimum inhibitory concentrations (mics) of the drug as well as those of other new quinolones and oral cephems were determined. the following conclusions were reached. 1. comparison of the mic distribution for strains isolated in 1987 with those in 1990 suggested a tendency toward an increase in the frequency of oflx-resistant ...19911960856
[chronic conjunctivitis caused by oral anaerobic germs].a patient with chronic, unilateral conjunctivitis is presented. the predominant bacterial flora consisted mainly of prevotella intermedia (formerly bacteroides intermedius) and peptostreptococcus micros. restriction endonuclease fingerprinting of genomic dna demonstrated that p. intermedia most probably originated from the oral cavity. in the oral cavity this species was recovered from the dorsum of the tongue, the tonsillar area and the periodontal pockets. topical treatment with various antibi ...19911758517
[a classic case of ludwig's angina].the aa. have the chance to review the etiology, the clinic and the management of this risky process, through a characteristic ludwig's angina of their own practice. a middle age man carrying an odontogenic inflammation developed a spreading infection into the deep neck tissues. the problem was successfully resolved with antibiotherapy and surgical drainage.19911781512
[effects of antibiotics and anti-bacterial components of preparations for local treatment of suppurative wounds on pathogens of odonto- genic infections].the results of identification and sensitivity assay of 156 strains of pathogens causing odontogenic infections are presented. in the sensitivity assay antibacterial drugs were used. 42.3 percent of the strains were obligate anaerobes belonging to bacteroides, fusobacterium, peptococcus, peptostreptococcus, veillonella and actinomyces. significant differences in the microbial sensitivity to the drugs used for general and local therapy were detected. there was observed high sensitivity of the obli ...19911805697
[empyema due to an anaerobic streptococcus in childhood].anaerobic infections are quite rare in pediatric age, being that, they affect only neonates and immunodepressed patients. we think to be somewhat interesting to describe the case of our patient, a 9 year old boy, unaffected by any predisposing factor, came under our observation because of a severe respiratory distress. he showed evident clinical and radiological signs of pleural effusion in the right lung, together with a gas coil in the upper field and a left mediastinal shifting. a thoracentes ...19911806927
microbiological study of hiv-related periodontitis.the subgingival microbiota in 14 persons with hiv-periodontitis was examined. subgingival plaque samples were collected with paper points, transported in vmga iii, and plated on anaerobic enriched brucella blood agar and various selective media. hiv-periodontitis sites revealed actinobacillus actinomycetemcomitans, wolinella recta, peptostreptococcus micros, and bacteroides intermedius, each averaging 7% to 16% of the cultivable subgingival flora in positive patients. high levels of spirochetes ...19912002434
metabolism of 1,4-dinitro-2-methylpyrrole, a mutagen formed by a sorbic acid-nitrite reaction, by intestinal bacteria.1,4-dinitro-2-methylpyrrole (dnmp), a mutagenic product formed by the interaction of two common food additives, sorbic acid and sodium nitrite, was transformed to 1-nitro-2-methyl-4-aminopyrrole (nmap) by human fecal mixtures and various intestinal bacterial strains. under anaerobic conditions the cell suspensions of actinomyces, bacteroides, clostridium, eubacterium, fusobacterium, and peptostreptococcus spp. demonstrated the nitroreduction activity. under aerobic conditions, only actinomyces a ...19912022195
predominant cultivable flora in pericoronitis.the predominant cultivable flora in pericoronitis was investigated by culturing pus from affected sites in 20 patients. twenty colonies were picked at random from non-selective plates and identified using conventional biochemical and physiological tests, analysis of metabolic end-products by gas chromatography and protein profile analysis. the most frequently isolated organisms were prevotella (bacteroides) intermedia, peptostreptococcus micros, veillonella species, fusobacterium nucleatum and s ...19911820571
bacteria in the apical 5 mm of infected root canals.ten freshly extracted teeth which had carious pulpal exposures and periapical lesions contiguous with the root apex were placed inside an anaerobic chamber and the apical 5 mm of the root canals cultured. in addition to anaerobic incubation, duplicate cultures were incubated aerobically. fifty strains of bacteria from the 10 root canals were isolated and identified. the most prominent bacteria cultured from the 10 root canals were actinomyces, lactobacillus, black-pigmented bacteroides, peptostr ...19911809801
tetracycline resistance in peptostreptococcus species.of 15 peptostreptococcus sp. strains isolated between 1975 and 1984, 13 hybridized with the tet k, tet m, or tet o determinant. a donor peptostreptococcus anaerobius strain carrying the three determinants could transfer tet m to p. anaerobius and fusobacterium nucleatum recipients but not to an enterococcus faecalis recipient, while neither tet k nor tet o was transferred.19911929341
reactivity of igg from explant cultures of periapical lesions with implicated microorganisms.the presence of igg in periapical inflammatory lesions suggests that immune responses participate in the disease process. the purpose of this investigation was to study the reactivity of igg from the supernatant fluids of explant cultures of periapical lesions with microorganisms implicated in infections of endodontic origin. ninety periapical lesions that had been contiguous with the apex of a root were removed and maintained in explant cultures. a dot-enzyme-linked immunosorbent assay (dot-eli ...19911940739
specificity of antibodies present in human periapical lesions.various classes of immunoglobulins have been found in human periapical lesions. the specificity of secreted antibodies against antigens egressing from the root canal system has yet to be thoroughly investigated. the purpose of this study was to test the specificity of antibodies present in human periapical lesions. human periapical biopsies were removed and cultured as organ culture explants. antibodies present in the lesions were extracted in the cell culture fluids. a modified enzyme-linked im ...19911940740
chronic conjunctivitis caused by oral anaerobes and effectively treated with systemic metronidazole plus amoxicillin.in this study, we report on a case of refractory, unilateral anaerobic conjunctivitis. the predominant anaerobic flora consisted of prevotella intermedia (formerly bacteroides intermedius) and peptostreptococcus micros. by using the technique of restriction endonuclease fingerprinting of genomic dna, it was shown that the p. intermedia likely originated from the oral cavity. topically applied antibiotics had failed to suppress the infection in the past. successful treatment was achieved after sy ...19911890173
defective neutrophil function in an insulin-dependent diabetes mellitus patients. a case report.the objective of this study was to evaluate the polymorphonuclear leukocyte (pmn) function in a poorly controlled adult insulin-dependent diabetic patient (iddm) with severe recurrent periodontitis, while describing the microbiological and clinical findings. chemotaxis, superoxide production, and phagocytosis and killing of porphyromonas (bacteroides) gingivalis by the iddm pmn were evaluated 1 week before treatment relative to a healthy, matched control. these analyses revealed a significant (p ...19911651389
in-vitro activity of temafloxacin against anaerobic bacteria: a comparative study.the antimicrobial activity of temafloxacin for 328 anaerobic bacteria was determined and compared to that of cefotetan, cefoxitin, ciprofloxacin and metronidazole. the wadsworth agar dilution technique using brucella-lysed sheep blood agar was used throughout. at the recommended breakpoint concentration 4 mg/l, temafloxacin inhibited 60/62 (97%) of the isolates of bacteroides fragilis and 82/87 (94%) of the isolates of other species of the b. fragilis group. ninety-six percent of the 24 isolates ...19911664827
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