antibodies to eubacterium and peptostreptococcus species and the estimated probability of crohn's disease.anaerobic coccoid rods belonging to species of eubacterium and peptostreptococcus agglutinate more frequently with sera from patients with crohn's disease than with sera from patients suffering from other diseases and from healthy subjects. results of agglutination test with four strains of coccoid anaerobes were used to estimate the probability that a patient suffers from crohn's disease. the data on healthy subjects and patients with crohn's disease were subjected to logistic discriminant anal ...19817252136
[infectious peptostreptococcus endocarditis after heart catheterization]. 19817266360
aerobic and anaerobic bacteria in tonsils of children with recurrent tonsillitis.tonsils were obtained from 50 children suffering from recurrent tonsillitis. patients' ages ranged from 2.5 to 17 years (mean 6 years); 29 were males and 21 females. the tonsils were sectioned in half after heat searing of the surface and the core material was cultured for aerobic and anaerobic microorganisms. mixed aerobic and anaerobic flora was obtained in all patients, yielding an average of 7.8 isolates (4.1 anaerobes and 3.7 aerobes) per specimen. there were 207 anaerobes isolated. the pre ...19817271131
adrenal abscess in a neonate due to gas-informing organisms: a diagnostic dilemma.a case of adrenal abscess due to bacteroides and peptostreptococcus in a 16-day-old infant is presented. the clinical manifestations consisted of a huge abdominal swelling associated with fever and lethargy. this is the first case report of an adrenal abscess due to gas-forming organisms, a finding which makes the preoperative diagnosis more difficult. surgical drainage, subtotal excision of the abscess wall under cover of antibiotics and preservation of the ipsilateral kidney were followed by s ...19817282048
[complication following the vaginal use of potassium permanganate for an abortion].complications after abortifacient use of potassium permanganate in a 22-year old woman are described. approximately 1 month prior to admission, the patient placed in the vagina a bag with potassium permanganate for 12 hours. gynecological examination showed stenosis of the vagina, enlargement of the uterus, and adhesions in the cervix and vagina. the patient underwent surgical separation of the vagina, daily irrigations of the vagina, and postoperative administration of ampilillin. microbiol ...19817304855
case report: livedo reticularis in endocarditis.the peripheral manifestations of bacterial endocarditis are manifold and provide diagnostic clues as to the onset, etiology and potential complications of the disease. petechiae, splinter hemorrhages, and osler's nodes are among the more common cutaneous signs of endocarditis thought to be secondary to an allergic vasculitis or, as in the case of osler's nodes, peripheral emboli. an asymmetric livedo reticularis rash was the initial presentation in a patient we recently treated with peptostrepto ...19817315865
extensive botryomycosis in a patient with diabetes and chronic active hepatitis.extensive botryomycosis was diagnosed in a patient with diabetes mellitus with chronic active hepatitis. peptostreptococcus organisms and staphylococcus aureus grew from the sulfur granule cultures. the patient's neutrophils showed notable inhibition of chemotaxis toward endotoxin-activated normal serum. the patient's serum also had an inhibitor to neutrophil chemotaxis, this inhibiting substance was probably antistreptolysin o. results of other tests of neutrophil function and skin tests to eva ...19817316536
[the absorption, excretion and influence on bowel flora of oral paromomycin sulfate (author's transl)].the absorption, excretion and influence on bowel flora of oral paromomycin sulfate (aminosidine, prm) were studied in ten normal volunteers taking a normal diet, and the following results were obtained. 1. serum levels of prm were observed 0.46 micrograms/ml at a half hour, 1.14 micrograms/ml at 1 hour, 1.48 micrograms/ml at 2 hours, 0.70 micrograms/ml at 4 hours, 0.29 micrograms at 6 hours and were almost faded out at 12 hours after 4 grams of oral administration. 2. during 0 approximately 2 ho ...19817321186
fatal aspiration pneumonia in an african elephant. 19817328011
determination of fmn and fad by fluorescence titration with apoflavodoxin. 19807374468
isolation, synthesis, and properties of 8-hydroxyflavins. 19807374472
bacterial studies of peritoneal cavity and postoperative surgical wound drainage following perforated appendix in children.this study reports bacterial specimens obtained from 112 children presenting with a ruptured appendix. additional samples were studied from 11 of these patients who developed a postoperative surgical draining wound. bacterial growth occurred in 100 peritoneal fluid specimens. anaerobic bacteria alone were present in 14 specimens, aerobes alone in 12, and mixed aerobic an anerobic flora in 74 specimens. there were 144 aerobic isolates (1.4 per specimen). the predominant isolates were: e. coli (57 ...19807406576
aerobic and anaerobic bacteriology of cervical adenitis in children.needle aspirates from 53 inflamed cervical lymph glands were studies for aerobic and anaerobic bacteria and mycobacteria. bacterial growth was achieved in 45 patients (85%). sixty-six bacterial isolates were recovered, averaging 1.5 isolates per specimen (0.8 aerobes and 0.7 anaerobes), with as many as 4 isolates in some specimens. aerobic organisms alone were recovered in 27 aspirates (60%) of the 45 culture-positive aspirates, anaerobic bacteria alone in 8 (18%), and mixed aerobic and anaerobi ...19807408372
['sterile' brain abscesses (author's transl)].in 16 cases of brain abscess (1970 to 1976) 10 could be identified to be bacteriologically sterile. however in 5 cases of "sterile" cerebral abscesses, where swabs were taken under anaerobic conditions, it was possible to isolate anaerobic bacteria in every case. it is concluded that in reality thse so-called "sterile" brain abscesses were also contaminated with anaerobes.19807415618
agglutinins to bacteria in crohn's disease.sera from patients with crohn's disease were tested for antibodies against organisms which are thought to cause inflammatory bowel disease in animals, or have been implicated in human crohn's disease. control sera were collected from healthy individuals and patients with ulcerative colitis. sera from crohn's disease and controls failed to agglutinate clostridium colinum or campylobacter sputorum subsp. mucosalis and two strains of mycobacterium paratuberculosis (m26 and m27). most of the sera ag ...19807429299
bactericidal effect of hydrogen peroxide is prevented by the lactoperoxidase-thiocyanate system under anaerobic conditions.streptococcus sanguis and peptostreptococcus anaerobius were exposed to various combinations of the components of the lactoperoxidase-thiocyanate-hydrogen peroxide system. the bactericidal effect of hydrogen peroxide was prevented under anaerobic conditions by lactoperoxidase together with thiocyanate, but not by lactoperoxidase or thiocyanate alone. thiocyanate was effective already at a molar ratio to hydrogen peroxide of 1:100.19807429633
effect of temperature, humidity and exposure to oxygen on the survival of anaerobic bacteria.the relative effect of humidity, temperature and exposure to oxygen on the survival of 11 strains of bacteriodes spp., fusobacterium necrophorum, eubacterium lentum, clostridium ramosum, peptococcus asaccharolyticus and peptostreptococcus anaerobius was evaluated. suspensions of the test strains were applied to membrane filters either in empty sterile petri dishes or on plates of non-nutrient agar to provide dry or moist conditions. the filters were stored aerobically or anaerobically in an atmo ...19807431377
[pleuro-pulmonary infections with anaerobic germs (author's transl)].the author presents his experience with the study of 42 patients with pleuro-pulmonary infection with anaerobic germs. he underlines the value of research tending to improve the conditions of sampling and culturing to ensure a bacteriological diagnosis and the setting of a suitable therapy.19807433351
anaerobic and aerobic bacteriology of decubitus ulcers in children.forty-two children with decubitus ulcers were studied using aerobic and anaerobic techniques. anaerobic bacteria were isolated in 21 (50%) of the patients, five times as the only isolates and 16 times mixed with aerobes. aerobes only were present in 20 (48%) of the patients. there were a total of 83 isolates, 46 aerobes and 37 anaerobes, with an average of two species per specimen (1.1 aerobes and 0.9 anaerobes). the predominant anaerobic isolates were gram-positive cocci (17), bacteroides fragi ...19807436139
[bacteriological examinations of liquid drawn from the pouch of douglas (author's transl)].the article reports on bacteriological examinations of 510 samples of liquid drawn from the pouch of douglas (excavatio rectouterina) during pelviscopy in patients not displaying any signs of inflammation. bacterial populations were found in 270 samples (= 52.9%). the results are subdivided according to aerobic and anaerobic types of pathogens and their incidence.19807450495
[endocarditis due to the anaerobe peptostreptococcus]. 19807450583
[pathology caused by anaerobic bacteria in pediatrics. our cases]. 19807453681
[a case of gas-producing brain abscess with subdural empyema: timing of the operation for brain abscess].a case of gas-producing brain abscess with subdural abscess was reported. an 18-year-old boy was admitted with a five-day history of vomiting and high grade pyrexia. plain skull roentgenograms demonstrated left frontal multiple gas bubbles. ct scan and mri showed that both brain and subdural abscesses contained gas in the left frontal area. antibiotics and glyceol were intravenously administrated. in serial ct scans, subdural abscess was not recognized, while brain abscess was enhanced in a ring ...19957477705
the antimicrobial activity of essential oils and essential oil components towards oral bacteria.a method for reproducibly determining minimal inhibitory concentrations and minimal bactericidal concentrations of plant extracts towards fastidiously and facultatively anaerobic oral bacteria, predicated upon measurements of optical densities in microtitre plate wells, was devised. the antimicrobial properties of some botanical oils were surveyed; of these, australian tea tree oil, peppermint oil, and sage oil proved to be the most potent essential oils, whereas thymol and eugenol were potent e ...19947478759
clinical and microbiological features of necrotizing fasciitis.the microbiological and clinical characteristics of 83 patients with necrotizing fasciitis (nf) treated over a period of 17 years are presented. bacterial growth was noted in 81 of 83 (98%) of specimens from patients with nf. aerobic or facultative bacteria only were recovered in 8 (10%) specimens, anaerobic bacteria only were recovered in 18 (22%) specimens, and mixed-aerobic-anaerobic floras were recovered in 55 (68%) specimens. in total, there were 375 isolates, 105 aerobic or facultative bac ...19957494032
budgerigar breeders' hypersensitivity pneumonitis presenting as chronic bronchitis with purulent sputum.a 49-year-old woman complained of cough and a purulent sputum (about 40 ml/day). she kept budgerigars for a period of 30 years, she first noticed a productive cough 10 years previously. her chest roentgenogram revealed a diffuse reticulonodular pattern. she was diagnosed as having a chronic type of budgerigar breeder's lung associated with chronic bronchitis. peptostreptococcus anaerobius was cultured from her sputum, bronchoalveolar lavage fluid, as well as from droppings of her budgerigar. thi ...19957496084
demonstration of human immunoglobulin g fc-binding activity in oral bacteria.nonimmune binding of immunoglobulins via the fc fragment may reduce opsonization and phagocytosis of bacteria and is thus considered a virulence factor. the aim of this study was to investigate a wide range of oral bacterial strains for the presence of human immunoglobulin g (igg) fc-binding activity. a total of 132 strains representing 40 different gram-positive and gram negative bacterial species were tested for igg fc-binding activity by using a fast and simple dot blot procedure with horsera ...19947496956
in vitro comparison of the effectiveness of three surface disinfectants.choosing a general purpose surface disinfectant for the dental office environment is difficult because of the wide range of products available, varying claims by manufacturers and contradictory reports in the literature regarding product efficacy. this study tested the antimicrobial effectiveness of diluted-for-use o-phenyl-phenol (omni), didecyl dimethyl ammonium (basic g) and isopropanol (virahol). products were tested for antimicrobial activity at 0, 12 and 24 hours, 3, 6 and 8 days. o-phenyl ...19947523623
techniques for controlling variability in gram staining of obligate anaerobes.identification of anaerobes recovered from clinical samples is complicated by the fact that certain gram-positive anaerobes routinely stain gram negative; peptostreptococcus asaccharolyticus, eubacterium plautii, clostridium ramosum, clostridium symbiosum, and clostridium clostridiiforme are among the nonconformists with regard to conventional gram-staining procedures. accurate gram staining of american type culture collection strains of these anaerobic bacteria is possible by implementing fixin ...19957538512
translation of trpg in bacillus subtilis is regulated by the trp rna-binding attenuation protein (trap).trpg of bacillus subtilis encodes a glutamine amidotransferase subunit that is involved in the synthesis of both folic acid and l-tryptophan. expression of trpg is negatively regulated by tryptophan even though this gene is located within a folic acid biosynthetic operon. examination of both transcriptional and translational gene fusions to lacz involving trpg and direct measurements of trpg mrna levels and trpg polypeptide accumulation demonstrated that translation of trpg is regulated by trypt ...19957543470
role of the reference laboratory in susceptibility testing of anaerobes and a survey of isolates referred from laboratories in england and wales during 1993-1994.methods for susceptibility testing of anaerobes and their patterns of susceptibility remain debatable. the responsibilities of the public health laboratory service anaerobe reference unit include identification of referred isolates; development of methods; monitoring of the occurrence of anaerobic infections; performance of taxonomic studies; maintenance of a reference collection; and monitoring of the antibiotic susceptibility of clinical isolates. selection of antibiotics for anaerobic infecti ...19957548547
bacteriology of chronic otitis media, chronic sinusitis, and paranasal mucopyocele in japan.a bacteriologic study of cases of chronic otitis media, chronic sinusitis, and paranasal mucopyocele diagnosed at gifu university hospital was performed between 1989 and 1992. bacteria were isolated from all of 13 samples of discharge from the ears of patients with chronic otitis media, all of 14 mucosal biopsy specimens from patients with chronic sinusitis, 8 of 10 aspirate samples from patients with chronic sinusitis, and 9 of 15 aspirates samples from patients with mucopyocele. cultures often ...19957548557
a retrospective review of cases of anaerobic empyema and update of bacteriology.we conducted a retrospective study to update the bacteriology of 46 cases of anaerobic empyema that were originally studied between 1976 and 1993 at the wadsworth anaerobic bacteriology clinical research laboratory (los angeles). anaerobic bacteriologic studies were completed for all 46 pleural fluid specimens, and aerobic bacteriologic studies were completed for 41 of these specimens. thirty-seven clinical charts were available for review. a total of 161 anaerobic isolates (3.5 per patient) rep ...19957548560
role of bacterial vaginosis in pelvic inflammatory disease.pelvic inflammatory disease (pid) is a frequent infection in sexually active young women and results in adverse sequelae, including tubal-factor infertility and ectopic pregnancy. in the 1970s investigations using culdocentesis demonstrated that anaerobic bacteria played an important role in the etiology of pid. this finding has subsequently been confirmed by studies utilizing laparoscopy and/or endometrial biopsy to obtain specimens directly from the upper genital tract (uterine cavity and fall ...19957548573
bacteriology of skin and soft-tissue infections: comparison of infections in intravenous drug users and individuals with no history of intravenous drug use.the bacteriology of cutaneous or subcutaneous abscesses (86 specimens) among intravenous drug users (ivdus) was compared with the bacteriology of abscesses (74 specimens) in patients with no history of intravenous drug use (non-ivdus). the ivdu abscesses yielded 173 aerobes and 131 anaerobes. staphylococcus aureus was the most common aerobe isolated (50% of specimens yielded this isolate), followed by "streptococcus milleri" (46%). the commonly encountered anaerobes were fusobacterium nucleatum ...19957548575
foot infections in diabetic patients: the role of anaerobes.the prevalence of anaerobic bacteria in cultures of specimens from foot infections in diabetic patients is dependent upon the method of obtaining the specimen, the care with which it is transported anaerobically, and the sophistication of the laboratory methods. the rate at which anaerobes are isolated with use of the best methods ranges from 74% to 95% of patients, but it is only 41% to 53% in clinical studies of patients with limb-threatening infection. the mean number of bacterial isolates fr ...19957548576
a phage display system for studying the sequence determinants of protein folding.we have developed a phage display system that provides a means to select variants of the igg binding domain of peptostreptococcal protein l that fold from large combinatorial libraries. the premise underlying the selection scheme is that binding of protein l to igg requires that the protein be properly folded. using a combination of molecular biological and biophysical methods, we show that this assumption is valid. first, the phage selection procedure strongly selects against a point mutation i ...19957549875
the microbiology and antibiotic treatment of peritonsillar abscesses.pus from 53 peritonsillar abscesses was cultured and associations between the microbiological results and clinical data were investigated with the aim of developing a clinical protocol for treatment. a positive culture grew in 85% of quinsies and of these 16% produced aerobes and 84% anaerobes. penicillin-resistant organisms were grown from 32% of patients and all but one of these organisms (haemophilus influenzae) was sensitive to metronidazole. there was no association between clinical present ...19957554331
microbiology of secondary bacterial infection in scabies lesions.aerobic and anaerobic bacteria were grown from specimens obtained from 30 children with secondarily infected scabies lesions. aerobic or facultative bacteria only were present in 14 (47%) patients, anaerobic bacteria only were present in 6 (20%) patients, and a mixed anaerobic-aerobic flora was present in 10 (33%) patients. fifty isolates were recovered (1.7 per specimen); 27 were aerobic or facultative bacteria and 23 were strict anaerobes. the predominant aerobic and facultative bacteria were ...19957559963
in vitro activity of bay y 3118, and nine other antimicrobial agents against anaerobic bacteria.the antibacterial activity of bay y 3118, a new chlorofluoroquinolone, was determined against 257 strains of anaerobic bacteria and compared with the activities of ofloxacin, ciprofloxacin, sparfloxacin, imipenem, cefoxitin, clindamycin, chloramphenicol, metronidazole, and ornidazole. overall, bay y 3118 was the most active agent tested against the bacteroides fragilis group. its activity (mic90, 0.5 mg/l) was 16-fold lower than that of sparfloxacin (mic90, 8 mg/l), and more than 100-fold lower ...19957562012
[in vitro activity of carbapenems (biapenem, imipenem and meropenem) and some other antibiotics against strict anaerobic bacteria].during 1994, the in vitro antibiotic susceptibility of 306 anaerobic bacteria was performed in 4 hospitals, by the reference agar dilution method. among the 129 b. fragilis group strains, only two b. fragilis strains were resistant to the three carbapenems and all beta-lactams, even combined with beta-lactamase-inhibitors while metronidazole resistance could not be detected. evolution in antibiotic resistance rates could be assessed only for piperacillin whose resistance rates increased to 20%. ...19957567117
changes in the core tonsillar bacteriology of recurrent tonsillitis: 1977-1993.microbiological studies of the core of tonsils removed from children with recurrent tonsillitis due to group a beta-hemolytic streptococci were conducted during three periods, with 50 patients in each period: 1977-1978 (period 1), 1984-1985 (period 2), and 1992-1993 (period 3). mixed flora were present in all tonsils, with 8.1 organisms per tonsil (3.8 aerobes and 4.3 anaerobes). the predominant isolates in each period were staphylococcus aureus, moraxella catarrhalis, peptostreptococcus species ...19957578726
recombinant proteins l and lg: efficient tools for purification of murine immunoglobulin g order to improve antibody purification methods, recombinant proteins l and lg were tested in the purification of murine monoclonal immunoglobulin g (igg) and its fragments. after affinity constant evaluation in different buffer systems, high-performance affinity chromatographic columns were prepared by coupling the proteins to affi-prep 10 resin and tested with eight different murine monoclonal antibodies and their fragments of different isotypes. affinity chromatographic experiments confirme ...19957581856
the role of cefotaxime in the treatment of surgical infections.this study examines the role of cefotaxime in the treatment of both gram-negative and gram-positive surgical infections. a dose of 2 g of cefotaxime will sustain peripheral compartment concentrations of 2.6, 3.9, 1.6, and 0.7 micrograms/ml for 6, 8, 10 and 12 h, respectively. therefore, the proportion of pathogens with a minimal inhibitory concentration (mic) below the peripheral compartment cefotaxime concentrations was assessed as a measure of therapeutic potential. it was observed that bacter ...19957587036
the ga module, a mobile albumin-binding bacterial domain, adopts a three-helix-bundle structure.we present the first study of the secondary structure and global fold of an albumin-binding domain. our data show that the ga module from protein pab, an albumin-binding protein from the anaerobic bacterial species peptostreptococcus magnus, is composed of a left-handed three-helix bundle. the helical regions were identified by sequential and medium range noes, values of nh-c alpha h coupling constants, chemical shift indices, and the presence of slowly exchanging amide protons, as determined by ...19957589548
role of escherichia coli p fimbriae in intestinal colonization in gnotobiotic rats.adherence via p fimbriae is associated with long-term persistence of escherichia coli in the human large intestine, but a causal relationship has not been proven. in the present study, germfree rats were colonized with a mixture of two isogenic e. coli strains, one p fimbriated and the other type 1 fimbriated. both types of fimbriae conferred adherence to rat colonic epithelial cells. with two mutant strains from a pyelonephritogenic isolate of serotype o75:k5:h-, the p-fimbriated strain 824 att ...19957591136
an approach to efficacy screening of mouthrinses: studies on a group of french products (i). staining and antimicrobial properties in vitro.there is a large and increasing number of oral hygiene products available to the general public. as such, it is difficult to demonstrate efficacy for all in long-term home-use studies. the aim of this project was to determine whether screening studies could position the activity and efficacy of a number of antiseptic mouthrinses, available in france, by comparison with an established product. this study reports the experiments in vitro. the products under test were, 4 containing chlorhexidine (e ...19957593703
factors affecting the occurrence of bacteremia associated with tooth extraction.this study examined the factors that affect the occurrence of bacteremia associated with tooth extraction and the kinds of bacteria causing this bacteremia. bacteremia was found in 132 (72.1%) of 183 patients who had one or more teeth extracted for various reasons. bacteremia occurred more frequently when teeth were extracted because of inflammatory dental diseases. the occurrence of bacteremia also increased with the number of teeth extracted and the age of the patients. when the volume of bloo ...19957594761
antibacterial effects of some root end filling addition to having good sealing ability, root end filling materials should "ideally" have some antibacterial activity to prevent bacterial growth. this investigation compared the antibacterial effects of amalgam, zinc oxide-eugenol, super eba and a mineral trioxide aggregate on nine facultative bacteria streptococcus fecalis, streptococcus mitis, streptococcus mutans, streptococcus salivarius, lactobacillus species, staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, and esc ...19957595152
mapping of the immunoglobulin light chain-binding site of protein l.protein l is a cell surface protein expressed by some strains of the anaerobic bacterial species peptostreptococcus magnus. the molecule binds specifically and with high affinity to immunoglobulins (ig) of a wide range of animal species. the ig-binding activity is mediated through five highly homologous domains, each 72 to 76 amino acid residues long, which interact with framework regions in the variable domain of ig light chains. the interaction does not interfere with the antigen binding capac ...19957608965
clinical features and aerobic and anaerobic microbiological characteristics of investigate the aerobic and anaerobic microbiological characteristics of cellulitis and correlate them with the infection site.19957611872
microbiologic and clinical study of polytetrafluoroethylene membranes for guided bone regeneration around implants.this study determined the microbiota of the mucosa- and implant-facing parts of expanded polytetrafluoro ethylene augmentation material, and the influence of major periodontopathogens on the healing process associated with guided bone regeneration around dental implants. seventeen patients with nine dehiscence and eight extraction defects were studied. prior to surgery and at membrane removal, microbial morphotypes, total viable counts, and the occurrence of selected microbial species were exami ...19957615319
prosthetic-valve endocarditis caused by peptostreptococcus anaerobius. 19957620041
periodontal microbiota of mobile and non-mobile teeth.the mechanism of accelerated periodontal destruction around teeth with occlusal trauma and increased mobility remains unclear. one possibility is that tooth mobility creates a subgingival environment conducive to overgrowth by periodontal pathogens. this study compared the subgingival microflora in mobile and non-mobile teeth of 35 adults on supportive maintenance therapy and 15 with untreated adult periodontitis. in each subject, subgingival paper-point samples were obtained from a mobile tooth ...19957623258
[nosocomial infections in present-day traumatological-orthopedic hospitals].nosocomial infection remains a significant problem in modern traumatology and orthopedics. staphylococcus prevails in its etiological pattern. the activation of microorganisms such as acinetobacter is noted. in addition to aerobic microorganisms, anaerobic bacteria, nonsporulating ones, play an important etiological role. anaerobic infection is seedy in 30.4% of patients' blood samples. great emphasis is placed on microbial adhesiveness in the pathogenesis of an infectious process. the authors' ...19957626997
a numerical taxonomic study of the gram-positive anaerobic cocci.clinical (101) and collection (26) strains of gram-positive anaerobic cocci were examined in conventional tests and pyrolysis mass spectrometry (pms). numerical classifications based upon conventional test reaction patterns (ctrps) and pms showed 27 and 22 clusters, respectively. cross-tabulation of cluster membership in the two classifications showed excellent correlation, with the combined classifications showing clear groups corresponding to the currently recognised species peptostreptococcus ...19957629855
[antibacterial activities of cefmenoxime against recent fresh clinical isolates from patients in sinusitis].in order to evaluate antimicrobial activity of cefmenoxime (cmx), minimum inhibitory concentrations (mics) of cmx and control drugs were determined against clinical isolates from patients of sinusitis that were obtained in our laboratory from october of 1993 to march of 1994. the results are summarized as follows; 1. cmx showed strong antimicrobial activities against streptococcus pneumoniae, haemophilus influenzae and moraxella subgenus branhamella catarrhalis that were 3 major aerobic bacteria ...19957637194
liver abscesses as initial presentation of crohn's disease. 19957639260
treatment of extraoral sinus tracts from traumatized teeth with apical periodontitis.when a draining lesion is encountered on the skin of the face, an endodontic origin should always be considered in differential diagnosis. non-surgical endodontic therapy, sometimes complimented by surgery, or extraction are the choices for the treatment of these cases. three cases of extraoral sinus tract on the chin caused by necrotic pulp of traumatized lower anterior teeth are presented. a paste consisting of calcium hydroxide and barium sulfate powder mixed with glycerin was used. usage of ...19957641626
bacteremia in conjunction with endodontic therapy.this study characterizes oral microorganisms believed to have spread from the root canal into the blood stream during and after endodontic therapy of teeth with asymptomatic apical periodontitis. microbiological samples were taken under aseptic conditions from the root canal of 26 single-rooted teeth in 26 patients. in the endodontic treatment of 13 of the patients (group 1), the first 3 reamers, sizes 15, 20 and 25, were used to a level 2 mm beyond the apical foramen. in the other 13 patients ( ...19957641631
microbiology of gastrostomy site wound infections in children.specimens of pus were obtained from gastrostomy site wound infections in 22 children. polymicrobial flora was found in 21 of the 22 wounds. aerobic or facultative bacteria only were isolated in eight (36%) instances and mixed aerobic-anaerobic flora were isolated from the other 14 (64%) wounds. a total of 102 bacterial isolates (57 aerobic and 45 anaerobic) and seven cultures of candida were obtained. the most frequent isolates were escherichia coli (16 isolates). peptostreptococcus spp. (14), e ...19957650731
multiple ligand interactions for bacterial immunoglobulin-binding proteins on human and murine cells of the hematopoetic lineage.a group of bacterial ig-binding surface proteins were studied: protein h and m1 are from streptococcus pyogenes and interact with igg, protein l is expressed by peptostreptococcus magnus and shows affinity for ig light chains, whereas protein lg is a chimeric construction combining the binding properties of protein l with the igg-binding activity of protein g from group c and g streptococci. proteins l and h coupled to sepharose were mitogenic for human peripheral blood lymphocytes (pbl) and mou ...19957660068
endoscopic treatment of acute frontal sinusitis: indications and limitations. 19957675495
positive and negative associations between bacterial species in dental root canals.significant associations have been previously reported between certain pairs of bacterial species isolated from human dental root canals. the aim of this study was to examine microbiologically a more extensive series of cases, with particular reference to obligate anaerobes which accounted for 64% of total isolations. a total of 65 different species was isolated and individual root canals yielded a maximum of eleven bacterial species. highly significant positive associations (p < 0.001) were fou ...19947700162
evaluation of the etest for antimicrobial spectrum and potency determinations of anaerobes associated with bacterial vaginosis and hundred ninety-seven anaerobic organisms (24 gardnerella vaginalis, 16 mobiluncus spp., 19 peptostreptococcus spp., 20 lactobacillus spp., 20 prevotella bivia/disiens, 81 bacteroides fragilis group, 12 clostridium spp., and five fusobacterium spp.) were processed by the etest (ab biodisk, solna, sweden) and a reference (brucella blood agar) method against 10 antimicrobial agents. for the bacterial vaginosis-associated pathogens, the etest was more reproducible and correlated acceptably with ...19947705035
intracranial complications of sinusitis in childhood.complications of sinusitis in children, such as intracranial abscess formation, are uncommon and are often clinically unremarkable in comparison to similar disease processes in adults. between 1983 and 1991, 443 children were admitted to children's national medical center in washington, dc, for treatment of sinusitis. fourteen of these children presented with intracranial extension of the infection and abscess formation. a retrospective review of these patients revealed that the risk of developi ...19957717619
microbiology of chronic suppurative otitis media in children in surabaya, indonesia.the aerobic and anaerobic microbiology of 38 children from surabaya, indonesia, who suffered from chronic suppurative otitis media (csom) was studied using strict microbiological methodology. a total of 106 isolates (49 anaerobic and 57 aerobic) were recovered. aerobic organisms alone were isolated from 11 (29%), anaerobic bacteria only in 4 (11%) and mixed aerobic and anaerobic flora were present in 23 (60%). the predominant organisms were peptostreptococcus sp., prevotella sp., bacteroides sp. ...19957729990
recombinant proteins l and lg. two new tools for purification of murine antibody fragments.several bacterial cell wall proteins, like proteins a and g, with valuable affinity for immunoglobulins have been discovered and are currently employed in immunological techniques. in 1988, protein l, a bacterial cell wall protein with ig-binding capacity, was isolated from the anaerobic bacterial species peptostreptococcus magnus. binding data with immunoglobulin fragments suggested that protein l could selectively bind the variable region of human kappa light chains. more recently a recombinan ...19947736533
surgical sepsis: constancy of antibiotic susceptibility of causative is well documented that antibiotic therapy exerts selective pressure on bacteria. conversion of bacteria from susceptible to resistant to antibiotics has been observed often during antimicrobial therapy. it has been postulated that human intestinal reservoirs facilitate communication of transposons that can transfer resistance determinants among various bacterial species.19957740421
adherence of bacteria to intraocular lenses: a prospective study.the study was designed to investigate the bacterial flora of the operating field during routine cataract surgery and the source of intraocular lens contamination during the surgery.19957742281
associations of specific bacteria with some endodontic signs and symptoms.although a wide range of bacterial species has been isolated from infected dental root canals it remains necessary to determine whether any particular group of such bacteria is associated with specific endodontic symptoms and clinical signs. in this study 30 root canals were examined microbiologically; of these, 14 were associated with pain, 20 with tenderness to percussion, 23 presented with wet root canals, seven with swelling, five with purulent exudate and four with a sinus. clinical and mic ...19947751061
[bone and joint infections due to unusual pathogens].between 1989 and 1993, 7 patients--2 men and 5 women from 19 to 70 years of age--with osteomyelitis due to an unusual organism were observed. 3 cases with salmonella typhimurium and one at a time with escherichia coli, peptostreptococcus, propionibacterium acnes and pasteurella multocida. 5 patients were treated operative accompanied with antibiotics and 1 without operation. 1 patient declined the indicated surgical therapy. the outcome were total healing in 3 cases, healing with ankylosis of th ...19957754663
necrotizing fasciitis of the head and neck with intrathoracic extension.we present the case history of a white male patient who initially presented in the emergency department with a peritonsillar abscess and subsequently returned several days later with necrotizing cervical fasciitis with intrathoracic extension. the successful treatment, utilizing surgical debridement and hyperbaric oxygen, will be discussed for this disease, which causes up to 50% or greater mortality.19957769648
[the prospects for using the new macrolide antibiotic azithromycin (sumamed) in the combined treatment of periodontitis].antibacterial activity of sumamed, a macrolide antibiotic, in respect of anaerobic microflora of the oral cavity, such as bacteroides, prevotella, porphyromonas, fusobacterium, peptostreptococcus, str. sanguis and staph. aureus, was assessed. the drug was found to be highly effective in respect of bacteroids (mpc90: 1,4 to 30 mg/mlmg/ml, this permitting the use of sumamed in the treatment of 9 patients with chronic generalized periodontitis in the exacerbation stage. bacteriological and clinical ...19957770869
crystalline surface protein of peptostreptococcus anaerobius.the surface ultrastructure of three anaerobic gram-positive cocci frequently encountered in oral infections, peptostreptococcus micros, p. magnus and p. anaerobius, was studied. the type strains of p. micros (dsm 20468) and p. anaerobius (atcc 27337), several clinical isolates of both species and the type strain of p. magnus (dsm 20470) were included. thin-sectioned cells studied by electron microscopy revealed a homogeneous layer outside the peptidoglycan layer in p. anaerobius. in p. micros an ...19957773401
timing of surgery in patients with infected spinal dermal sinuses: report of two the past, if infection was present at the time a spinal dermal sinus was discovered, the sinus opening and cerebrospinal fluid were sterilized with a course of appropriate antibiotic therapy prior to the surgical intervention. the cases of two children with an infected spinal dermal sinus are reported here. one patient developed acute paraplegia during the initial stage of antibiotic therapy, and the second patient, who was admitted only with signs of fever and irritability, experienced after ...19957773970
periodontal findings in spouses. a clinical, radiographic and microbiological study.clinical, radiographic and microbiological examination of periodontal conditions was carried out in 2 groups of married couples to assess similarities between husband and wife. the diseased probands (n = 10) exhibited advanced periodontitis and the healthy ones (n = 10) were periodontally normal. the clinical examination comprised the assessment of plaque, probing pocket depths, gingival bleeding on probing, suppuration, supragingival and subgingival calculus. the extent and type of alveolar bon ...19957775676
sinusitis and bronchial asthma in children.sinus disease has been assumed to exist in children with respiratory tract allergies. we consecutively evaluated 375 patients (245 male, 130 female) with childhood onset asthma, between 5 and 15 years of age (mean 7.8 years of age). abnormal waters radiographs were found in 205 patients (54.7%) which included mucosal wall thickening greater than 6 mm (67.3%), complete opacification (22.9%) and air-fluid levels (9.8%). the level of immunoglobulin (ig) e was found to be much higher in non-sinusiti ...19957778440
role of anaerobic bacteria in chronic otitis media and cholesteatoma.otitis media (om), a common infection in children, can cause significant morbidity. selection of the most appropriate treatment regimen directed against the pathogens responsible for the om can minimize complications. the most frequently isolated bacteria from chronic om are staphylococcus aureus, pseudomonas aeruginosa and anaerobic bacteria. the predominant anaerobes are peptostreptococcus spp., pigmented prevotella and porphyromonas spp., bacteroides spp. and fusobacterium spp. many of the or ...19957782173
effect of different combinations of antibiotics on experimental septic peritonitis in rabbits.this study was undertaken to evaluate the efficacy of different combinations of antimicrobial agents in the treatment of experimental septic peritonitis. two hundred rabbits, divided into ten groups of 20 rabbits each, were used. septic peritonitis was provoked in two stages: treatment of animals by mechanical lavage or antibiotics was performed during the first stage; blood cultures, isolation and identification of aerobic and anaerobic bacteria in the peritoneal cavity were performed during th ...19947819597
[treatment of puerperal endometritis. evaluation of the efficacy and safety of clindamycin + gentamycin vs. penicillin + chloramphenicol + gentamycin].this was a prospective, single-blind, comparative study in patients with diagnosis of puerperal endometritis, carried out at the loayza hospital in lima, peru. the objective of this study was to evaluate the efficacy and safety of clindamycin and gentamicin in the management of endometritis vs. penicillin, chloramphenicol and gentamicin for 10 days. sixty-five patients were enrolled and 62 were evaluable for efficacy. both treatment groups were comparable in the pre-treatment period in terms of ...19947821833
on the interaction between single chain fv antibodies and bacterial immunoglobulin-binding proteins.using four bacterial immunoglobulin-binding proteins, we have analyzed the binding characteristics of a panel of 34 human single chain fv antibodies, expressed in e. coli and with known specificity and sequence. several of the single chain fv antibodies showed affinity for staphylococcal protein a and peptostreptococcal protein l, but not for the streptococcal proteins g or h. the affinity of the binding was higher for protein l (4.5 and 1.4 x 10(9) m-1) than for protein a (7.7 and 6.7 x 10(8) m ...19947822821
[susceptibility of strict anaerobic bacteria to antibiotics in france: a multicenter study].during 1992, the in vitro antibiotic susceptibility of 462 anaerobic bacteria was performed in 7 hospitals, by the reference agar dilution method. among the 222 bacteroides fragilis group strains, only one bacteroides fragilis strain was resistant to imipenem and all bêtalactams, even combined with bêtalactamase-inhibitors while metronidazole resistance could not be detected. one major outer membrane protein (probably a porin) was lacking in some of the six amoxicillin-clavulanic acid resistant ...19947824321
in vitro activity of du-6859a against anaerobic bacteria.the activity of a new quinolone agent, du-6859a, against 330 strains of anaerobic bacteria was determined by using the national committee for clinical laboratory standards-approved wadsworth brucella laked blood agar method; the activity of du-6859a was compared with those of amoxicillin-clavulanate (2:1), chloramphenicol, ciprofloxacin, clindamycin, fleroxacin, imipenem, lomefloxacin, metronidazole, sparfloxacin, and temafloxacin. du-6859a and chloramphenicol inhibited all of the isolates at co ...19947840599
increased in vitro activity of ceftriaxone by addition of tazobactam against clinical isolates of anaerobes.a total of 461 clinical strains of anaerobes were tested using a broth microdilution test to determine the activity of the combination of ceftriaxone and tazobactam and other antimicrobials against these isolates. ceftriaxone was combined with tazobactam in ratios of 1:1, 2:1, 4:1, and 8:1 and twofold dilutions of ceftriaxone in constant concentrations to tazobactam of 2, 4, 8, 16, and 32 micrograms/ml. against beta-lactamase-producing strains of the bacteroides fragilis group, b. capillosus, an ...19947851086
microbiota of rapidly progressive periodontitis lesions in association with clinical parameters.the microbial population in 73 rapidly progressive periodontitis (rpp) lesions in 10 young adults aged 25 to 35 years (5 males, 5 females) was studied in relation to the clinical parameters probing depth, bleeding on probing, and suppuration, which were recorded at the sampled sites. porphyromonas gingivalis was found to predominate (26.7%) in 73 periodontal lesions with clinical probing depth > 6 mm, followed by bacteroides forsythus (23.6%), and prevotella intermedia (15.7%). the prevalence of ...19947853132
peptostreptococcal infection in children.680 peptostreptococcus sp. (ps) were recovered from 598 (34%) of 1,750 specimens obtained from 554 patients. they included 103 ps asaccharolyticus, 74 ps magnus, 56 ps prevotii, 51 ps micros, 46 ps anaerobius, 11 ps morbilorum, and 10 ps saccharolyticus. most infections were polymicrobial (in 553 instances or 92%) but in 45 (8%), ps were recovered in pure culture. most ps were isolated from abscesses (237), ears (104), peritoneal fluid (95), lung infections (66), bone (30) and sinuses (24). pred ...19947855547
bacteriological study of bronchoalveolar lavage in immunocompromised hosts.sixty bronchoalveolar lavage samples collected from lung cancer patients attending the pulmonary tuberculosis and chest diseases unit of the kasturba medical college hospital, manipal; were cultured for both the aerobic and anaerobic organisms. fifty nine samples yielded bacteria in pure culture. pseudomonas aeruginosa (34.8%) was the commonest aerobe, peptostreptococcus was the commonest anaerobe (45.2%) isolated. bacteroides fragilis was isolated in (23.8%) of cases. gentamycin was found to be ...19947868174
the gingival immune response to periodontal pathogens in juvenile periodontitis.a gingival explant culture system was utilized to evaluate the reactivity of local immunoglobulins produced by juvenile periodontitis tissue. gingival explant culture supernatant fluids were screened, via a standardized dot-immunobinding assay, for antibodies reactive to: actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, eikenella corrodens, peptostreptococcus micros, peptostreptococcus anaerobius, capnocytophaga ochracea, eubacterium no ...19947870467
major methodology-dependent discordant susceptibility results for bacteroides fragilis group isolates but not other anaerobes.two standardized susceptibility test methods, a broth microdilution (bmd) and agar dilution (ad) method were performed on a total of 441 clinical isolates of anaerobes with ceftizoxime, cefotaxime, ceftriaxone, cefoxitin, piperacillin, and metronidazole. against the 339 strains of the bacteroides fragilis group bmd minimum inhibitory concentration (mic) values were lower than those from ad testing for all the beta-lactams. overall for the b. fragilis group and the beta-lactams, the mode mic valu ...19947874880
advanced periodontitis associated with larsen's syndrome: case report.larsen's syndrome is a genetic disorder of connective tissue characterized by multiple large-joint dislocations, abnormalities of hands and feet, and anomalous facial features. although findings involving multiple systems have been added to its clinical and pathological entity, oral pathology besides cleft palate has not been reported. we report here advanced periodontitis with severe gingival inflammation in a 29-year-old larsen's syndrome patient. bacterial culture of subgingival plaque identi ...19957899108
study of gene transfer in vitro and in the digestive tract of gnotobiotic mice from lactococcus lactis strains to various strains belonging to human intestinal flora.the use of genetically modified organisms (gmo) in dairy products requires evaluation of the dna transfer capacity from such organisms among the human intestinal microflora. thus, both in vitro and in vivo [in the digestive tract (dt) of mice] transfer from lactococcus lactis donor strains of the conjugative plasmid pil205 (cmr) and the non-conjugative plasmid pil253 (emr) to: (1) recipient strains isolated from human faecal flora bacteroides sp., bifidobacterium sp., peptostreptococcus sp. (str ...19947921350
anaerobic susceptibility testing. slight differences in inoculum size can make a difference in minimum inhibitory the present study, we compared the actual inoculum density from inoculated broth microdilution wells to the targeted inoculum size (10(5) cfu/well) when the inoculum was prepared using a mcfarland nephelometer to achieve the standard density. three target inoculum sizes (10(5), 5 x 10(5), and 10(6) cfu/well) were used to compare the effect of slight inoculum size increases of both atcc and clinical strains of anaerobes on mics of various antimicrobials. actual colony counts of bacteroides fra ...19947924212
peritonitis following endoscopic polypectomy in a peritoneal dialysis patient: the need for antibiotic prophylaxis. 19947927824
evaluation of minocycline and cefuzonam for antimicrobial activity against clinical isolates.the antibacterial activity of minocycline (mino) and that of cefuzonam (czon) were assessed with clinical isolates of 19 species, and compared with that of other antibiotics. mino was highly active against methicilli-sensitive staphylococcus aureus (mssa), neisseria gonorrhoeae, moraxella (branhamella) catarrhalis, haemophilus influenzae, helicobacter pylori, flavobacterium meningosepticum, acinetobacter calcoaceticus, peptostreptococcus spp. and propionibacterium acnes, but not as effective aga ...19947933531
the functional units of a peptostreptococcal protein l.protein l is a cell-surface protein from peptostreptococcus which interacts with immunoglobulin kappa light chains. a gene from peptostreptococcus strain 3316 coding for protein l and fragments thereof were expressed in escherichia coli. the peptides were examined for binding to immunoglobulin and serum albumin. the four c units were shown to be responsible for binding to immunoglobulin and the four d units for binding to albumin. this protein l molecule therefore binds to albumin at a site sepa ...19947934898
changes in root canal microbiota during the development of rat periapical lesions.periapical lesions are reproducibly induced in rats by pulp exposure and infection from the oral cavity. lesions expand rapidly between day 7 and day 15-20 (active phase), with slowed expansion thereafter. in the present study we characterized the root canal microbiota present during the active phase of lesion development in this system. the mandibular first molars of sprague-dawley rats were exposed on day 0. the teeth were extracted after 7 days (n = 10 animals) and 15 days (n = 10), and the m ...19947936717
diagnosis and management of abscesses in the basal ganglia and thalamus: a survey.a review is made of the current management strategies of abscesses in basal ganglia and thalamus, based on a review of the literature and three of our own cases. clinical picture, aetiology, diagnostic, surgical treatment and outcome are discussed. stereotactic abscess puncture in combination with temporary drainage and rinsing of the abscess cavity in combination with systemic medication of antibiotics has become the management of choice with satisfactory results.19947942190
three-dimensional solution structure of an immunoglobulin light chain-binding domain of protein l. comparison with the igg-binding domains of protein g.protein l is a multidomain protein expressed at the surface of some strains of the anaerobic bacterial species peptostreptococcus magnus. it has affinity for immunoglobulin (ig) through interaction with framework structures in the variable ig light chain domain. the ig-binding activity is located to five homologous repeats called b1-b5 in the n-terminal part of the protein. we have determined the three-dimensional solution structure of the 76 amino acid residue long b1 domain using nmr spectrosc ...19947947810
study on treatment of bacterial vaginosis with oral administration of metronidazole or cefdinir.bacterial vaginosis (bv) is considered to be one of the most common vaginal infections in women. fifteen symptomatic women with bv were enrolled in this study. ten patients with the diagnosis of bv were treated with 10 days oral administration of metronidazole (mtn), 500 mg twice a day, and five patients with cefdinir (cfdn), 300 mg three times a day. in the mtn therapy, the rate of abnormal vaginal discharge subjectively decreased from 100 to 60%, the rate of abnormal vaginal discharges objecti ...19947956461
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