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sodium-dependent succinate decarboxylation by a new anaerobic bacterium belonging to the genus peptostreptococcus.an anaerobic bacterium was isolated from a polluted sediment, with succinate and yeast extract as carbon and energy sources. the new strain was gram-positive, the cells were coccal shaped, the mol% g+g content of the genomic dna was 29, and the peptidoglycan was of the l-ornithine-d-glutamic acid type. comparative sequence analysis of the 16s rrna gene showed the new strain to belong to the genus peptostreptococcus. succinate, fumarate, pyruvate, 3-hydroxybutyrate and lysine supported growth. su ...19968836437
[aerobic and anaerobic bacterial flora in acute abdominal illnesses].366 specinmens taken from patients suffering from acute abdominal illnesses who had undergone operations were examined. bacterial pathogens were isolated from 318 specimens. aerobic bacteria were isolated from 53.8% specimens. anaerobic bacteria were isolated from 12.6% specimens. mixed bacterial flora were isolated from 33.6% specimens. among aerobic bacteria e. coli and streptococcus faecalis were most often isolated. among anaerobic bacteria bacteroides fragilis, peptostreptococcus spp. and p ...19958833932
the dynamics of microbial colonization of barrier membranes for guided tissue regeneration.the microbial colonization of expanded polytetrafluoroethylene membrane by putative periodontopathogens at 3 minutes of intraoral manipulation was determined in 42 patients with 42 mandibular posterior two- to three-wall defects. twenty patients exhibited no periodontal pockets of > or = 5 mm, other than the study site, and low levels of pathogens (group a). twenty-two patients revealed multiple periodontal pockets of 5 mm or more and numerous pathogens (group b). within the preceding 3 months o ...19968832481
[antimicrobial activities of tosufloxacin against recently obtained clinical isolates].to evaluate antimicrobial activities of tosufloxacin (tflx), minimum inhibitory concentrations (mics) of tflx and other drugs were determined against clinical isolates that were obtained in our laboratory from february of 1993 to january of 1994 (period i), and from february of 1995 to january of 1996 (period ii). the results are summarized as follows: 1. compared to mic90's reported by others for tflx in the 1980's, those obtained by us against beta-streptococci, streptococcus pneumoniae, pepto ...19968828073
changing anaerobic spectrum in suppurative lung disease: a case report.a spectrum of three different anaerobes were isolated from a debilitated patient with suppurative lung disease, within a two-year period. repeated isolation from three consecutive samples and symptomatic relief with metronidazole provide clinical evidence of anaerobic lung infection. this case emphasizes the importance of anaerobic culture in cases of protracted pulmonary suppurative disease.19968822645
a purgative action of barbaloin is induced by eubacterium sp. strain bar, a human intestinal anaerobe, capable of transforming barbaloin to aloe-emodin anthrone.orally administered barbaloin (100 mg/kg) did not induce any diarrhea in male wistar rats, in spite of severe diarrhea with sennoside b (40 mg/kg). also, in gnotobiote rats mono-associated with peptostreptococcus intermedius, a human intestinal anaerobe capable of reducing sennidins to rhein anthrone, barbaloin did not induce diarrhea; the faecal water content (71.9%) 8 h after the administration of barbaloin was not increased, compared with that (73.9%) just before the treatment. however, sever ...19968820926
amplified expression and large-scale purification of protein l'.the gene fragment (ppl') encoding the functional unit of peptostreptococcus protein l was isolated using pcr and expressed in e. coli. as the gene fragment lacked its own promoter, the 5' pcr primer was designed to incorporate an nde1 restriction site (catatg) into the gene. this enabled the gene to be cloned in frame into an nde1 restriction site immediately downstream of a trp promoter. to prevent read through, a stop codon was introduced into the 3' primer. expression of ppl' was up to 27% to ...19968818265
bacteriologic findings in tonsillitis and pericoronitis.bacteriologic samples from 31 young men were cultured quantitatively for aerobes and anaerobes; these samples included 31 specimens of tonsils (16 infected and 15 healthy), 16 specimens from pericoronal pockets of lower third molars (11 infected and 5 symptom-free), and 6 postoperative specimens from lower-third-molar extraction sockets. anaerobes were isolated more often from infected third molars than from infected tonsils (14.5 isolates vs. 8.4 isolates, respectively; p < .001). infected tons ...19968816129
clinical, microbiological and immunological profile of healthy, gingivitis and putative active periodontal subjects.thirteen periodontally healthy subjects were monitored clinically for 6-12 months. clinical measurements at 6-weekly intervals included duplicate pd measurements, presence of plaque, redness, and bleeding on probing. baseline measurements consisted of 2 visits 1 wk apart. microbial samples were taken from 11 of the subjects who had completed at least 8 months of monitoring. levels of serum antibodies to 12 periodontal species were determined from 10 subjects. standard deviations of replicate pd ...19968814590
microbiology of periapical abscesses and associated maxillary sinusitis.aspirate of pus from 5 periapical abscesses of the upper jaw and their corresponding maxillary sinusitis were studied for aerobic and anaerobic bacteria. polymicrobial flora were found in all instances, where the number of isolates varied from 2 to 5. anaerobes were recovered from all specimens. the predominant isolates were prevotella sp., porphyromonas sp., fusobacterium nucleatum, and peptostreptococcus sp. concordance in the microbiological findings between periapical abscess and the maxilla ...19968794972
microbiology of acute purulent pericarditis. a 12-year experience in a military hospital.to study the aerobic and anaerobic micro-biological and clinical characteristics in 15 cases of acute pericarditis treated over a 12-year period.19968790081
susceptibilities of bovine summer mastitis bacteria to antimicrobial agents.the susceptibility to 9 antimicrobial agents of 32 aerobic bacterial isolates and to 10 antimicrobial agents of 37 anaerobic bacterial isolates from 23 cases of bovine summer mastitis (16 actinomyces pyogenes isolates, 8 streptococcus dysgalactiae isolates, 3 s. uberis isolates, 3 s. acidominimus isolates, 2 streptococcus spp., 15 peptostreptococcus indolicus isolates, 10 fusobacterium necrophorum isolates, and 12 isolates of anaerobic gram-negative rods) was determined by the agar dilution meth ...19968787898
[in vitro activities of sulopenem, a new parenteral penem, against anaerobes].in vitro activities of sulopenem, a novel parenteral penem, was compared with those of imipenem, flomoxef, cefuzonam, cefoperazone and sulbactam/ampicillin against 66 reference strains (19 genera, 61 species) and 392 recent clinical isolates of anaerobic bacteria and fastidious aerobic bacteria. sulopenem had a very broad spectrum against anaerobic bacteria. in general, this compound was active against anaerobic reference strains with mics of < or = 0.78 micrograms/ml, while being the least acti ...19968786627
a risk-benefit assessment of teicoplanin in the treatment of infections.teicoplanin is a glycopeptide antibiotic whose activity is selectively oriented against gram-positive aerobic and anaerobic bacteria, including staphylococcus aureus, coagulase-negative staphylococci, clostridium difficile, peptostreptococcus spp. and corynebacterium jeikeium; such activity is affected by neither methicillin resistance nor beta-lactamase production. teicoplanin is not significantly absorbed from the gastrointestinal tract; consequently, it has to be administered intravenously (e ...19958785019
cloning, expression and purification of ppl-1, a kappa-chain binding protein, based upon protein l from peptostreptococcus magnus.protein l is a multi-domain, cell wall constituent of certain strains of peptostreptococcus magnus, which binds to the variable domain of the l-chains of ig. a gene fragment which codes for a single ig-binding domain of protein l (ppl-1) was cloned into a modified pkk223-3 vector and over-expressed in e. coli jm103. a rapid protein purification protocol is described. in these studies, purified ppl-1 was immobilised on to an agarose gel and tested against an array of igs and ig fragments. it was ...19958767928
direct evidence for a two-state protein unfolding transition from hydrogen-deuterium exchange, mass spectrometry, and nmr.we use mass spectrometry in conjunction with hydrogen-deuterium exchange and nmr to characterize the conformational dynamics of the 62-residue igg binding domain of protein l under conditions in which the native state is marginally stable. mass spectra of protein l after short incubations in d2o reveal the presence of two distinct populations containing different numbers of protected protons. nmr experiments indicate that protons in the hydrophobic core are protected in one population, whereas a ...19968762137
[in vitro activity of a new fluoroquinolone, marbofloxacin (ro 09-1168) against strictly anaerobic bacteria and some bacteria from human fecal flora].as recommended by the eec, the in vitro activity of a veterinary quinolone has to be evaluated on human strains isolated from the gut and especially against anaerobes. thus, the mics for marbofloxacin were determined by the reference agar method (norma nccls m11 a3) using wilkins chalgren medium for the 124 anaerobic strains and mueller hinton agar for e. coli, proteus, enterococcus and lactobacillus. on the whole aerobes, the activity of marbofloxacin was equal or slightly greater to that of of ...19968758470
[antibiotic sensitivity of anaerobic bacteria isolated during one year (1993)].the aim of this study was to determine the antimicrobian susceptibility of the anaerobe bacteria isolated from clinical samples in the authors' department in 1993.19968756216
polymerase chain reaction-based detection of bacteria in semen.to determine if the presently used bacterial detection techniques provide accurate and complete profiles of microorganisms found in human semen.19968751749
bacteriological epidemiology and treatment of bacterial vaginosis.one hundred symptomatic women with clinical bacterial vaginosis (bv) were enrolled in this study. the bacteriological epidemiology of bv and efficacy of oral or vaginal administration of antimicrobial agents for treatment of bv were investigated. the epidemiology of bv was investigated before antimicrobial therapy. fifty patients were treated with oral administration of metronidazole (mtn), 500 mg twice a day, and 50 patients with 2% clindamycin (cldm) phosphate in a vaginal cream, 5 g once a da ...19968751270
crystallization and x-ray analysis of a single fab binding domain from protein l of peptostreptococcus magnus.protein l is a multidomain cell wall constituent of certain strains of peptostreptococcus magnus which binds to the variable domain of immunoglobulin kappa-light chains. a single immunoglobulin-binding domain of m(r) = 9000 from this protein has been isolated and crystallized. the crystals are of space group p4(2)2(1)2, with cell dimensions a = b = 66.9 a, c = 68.3 a, and diffract to at least 2.2 a resolution. the asymmetric unit of the crystal contains two molecules of the protein l domain, rel ...19958749861
comparison of spiral gradient endpoint and agar dilution methods for susceptibility testing of anaerobic bacteria: a multilaboratory collaborative evaluation.a multilaboratory collaborative study was carried out to assess the utility of the spiral gradient endpoint (sge) method for the determination of the antimicrobial susceptibilities of anaerobes and to evaluate the equivalence of the mics obtained by the sge method with those obtained by the reference agar dilution method of the national committee for clinical laboratory standards. the standard deviation of the mic obtained by the sge method for the five participating laboratories was +/- 0.26 of ...19968748295
utility of 5 major putative periodontal pathogens and selected clinical parameters to predict periodontal breakdown in patients on maintenance care.the predictive utility of 5 major putative periodontopathic microbial species, "superinfecting" organisms, and several clinical periodontal parameters were assessed relative to periodontitis recurrence over a 12-month period in 78 treated adult patients participating in a 3-month maintenance care program. at baseline, pooled subgingival microbial samples were collected from each patient, and whole-mouth evaluations of probing depth, relative periodontal attachment level, furcation involvement, a ...19968739166
response to periodontal therapy in patients with high or low levels of p. gingivalis, p. intermedia, p. nigrescens and b. forsythus.in a previous study, subjects receiving either adjunctive tetracycline or augmentin showed, on average, more attachment level gain 10 months post-therapy than subjects receiving either ibuprofen or a placebo, although some subjects in each treatment group showed loss of attachment post-therapy. since differences in treatment response might have been due to differences in the subgingival microbiota, the response to different therapies in subjects with different pre-therapy subgingival microbiotas ...19968739165
antibacterial activity of quinupristin/dalfopristin. rationale for clinical use.most gram-positive organisms are highly susceptible to the streptogramin, quinupristin/dalfopristin (rp 59500; synercid). minimum inhibitory concentrations for 90% of isolates (mic90) were < or = 1 mg/l for staphylococcus aureus, s. epidermidis, s. haemolyticus, streptococcus pneumoniae, s. pyogenes and listeria monocytogenes. importantly, quinupristin/dalfopristin shows similar activity against methicillin-susceptible and -resistant strains of s. aureus, and streptococci with benzylpenicillin ( ...19968724814
in vitro activity of the tricyclic beta-lactam gv104326.gv104326 is a novel tricyclic beta-lactam (a trinem or, formerly, tribactam). the in vitro activity of gv104326 was compared with those of cefuroxime, cefixime, amoxicillin, amoxicillin-clavulanic acid, cefpirome, and ciprofloxacin. gv104326 had in vitro activity generally similar to that of cefixime against members of the family enterobacteriaceae (mic at which 90% of the isolates are inhibited [mic90], < or = 2 micrograms/ml), with cefuroxime and amoxicillin-clavulanic acid being 8- to 32-fold ...19968723475
anaerobic septic bursitis: case report and review. 19968722967
identification of interdomain sequences promoting the intronless evolution of a bacterial protein family.in the evolution of eukaryotic genes, introns are believed to have played a major role in increasing the probability of favorable duplication events, chance recombinations, and exon shuffling resulting in functional hybrid proteins. as a rule, prokaryotic genes lack introns, and the examples of prokaryotic introns described do not seem to have contributed to gene evolution by exon shuffling. still, certain protein families in modern bacteria evolve rapidly by recombination of genes, duplication ...19968710897
pyomyositis in children, caused by anaerobic bacteria.the author describes the microbiology and clinical features of six pyomyositis infections in children, which yielded anaerobic bacteria. anaerobic bacteria alone were recovered in four instances, and they are mixed with facultative bacteria in two. there were 15 bacterial isolates (13 anaerobic, 2 facultative). the bacteria were peptostreptococcus sp (5 isolates), bacteroides fragilis (3), clostridium sp (2), fuso-bacterium nucleatum (1), prevotella sp (1), bateroides sp (1), streptococcus pyoge ...19968708910
microbiology of subperiosteal orbital abscess and associated maxillary sinusitis.aspirate of pus from eight subperiosteal orbital abscesses (spoas) and their corresponding infected sinuses were studied for aerobic and anaerobic bacteria. polymicrobial flora was found in all instances, and the number of isolates varied from two to five. anaerobes were recovered from all specimens. the predominant isolates were peptostreptococcus spp, prevotella spp, fusobacterium spp, staphylococcus aureus, and microaerophilic streptococci. concordance in the microbiological findings between ...19968699891
[a case of the greater omentum abscess due to peptostreptococcus ssp].a case of the greater omentum abscess due to peptostreptococcus ssp. is reported. a 63-year-old male was admitted to heisei memorial hospital with a complaint of abdominal pain. an intraperitoneal mass measuring 6 cm in diameter was detected by ct and ultrasonogram. he was diagnosed as having an intraperitoneal abscess. after antibiotics therapy by cefotiam, laparotomy was performed and an abscess about 7 cm in diameter at the edge of the greater omentum was found. there was no evidence of perfo ...19968699102
fetal fibronectin and microorganisms in vaginal fluid of healthy pregnant women.to determine fetal fibronectin in vaginal fluid from healthy women during uncomplicated pregnancy and to investigate possible relationships to gestational age and the vaginal microflora.19968693926
pneumonia in mechanically ventilated children.the quantitative aerobic and anaerobic microbiology of bronchial aspirates, obtained using protective brush catheters, from 10 children with ventilator-associated pneumonia, is presented. aerobic or faculative organisms only were isolated in 1 child, anaerobic bacteria only in 3, and aerobic mixed with anaerobic bacteria in 6. there were 10 aerobic or faculative and 17 anaerobic isolates. the predominant aerobes were pseudomonas aeruginosa (2 isolates) and klebsiella sp. (2). the predominant ana ...19958685643
[diagnosis and treatment of local forms of surgical anaerobic non-clostridial infection].145 patients with such kind of surgical infection have been taken to clarify the cause of it. aerobic agents were the cause in 16.6% and anaerobic nonclostridial agents--in 33.1% of all cases. the mixed flora was the cause in 44.1%. the most important place among anaerobic nonclostridial infections belongs to bacteroids (38.3%), peptostreptococcus (27.3%) and the peptococcus (17.2%). one must use wide-spectrum antibiotic for a treatment of a such kind of infection tiel the usults of microbiologi ...19968683929
[computed tomographic demonstration of intraspinal air after an infection with peptostreptococci]. 19968679984
bacteremia shortly after placental separation during cesarean delivery.to assess frequency, risk factors, and microbiology of bacteremia within 15 minutes of placental separation during cesarean delivery.19968677085
isolation of non-sporing anaerobic rods from infections in children.from 1974 to 1994, 2033 microbiological specimens from children were submitted for cultures for anaerobic bacteria. fifty-seven isolates of bifidobacterium spp. were obtained from 55 (3%) children, 67 isolates of eubacterium spp. from 65 (3%) children and 41 isolates of lactobacillus spp. from 40 (2%) children. most bifidobacterium isolates were from chronic otitis media, abscesses, peritonitis, aspiration pneumonia and paronychia. most eubacterium isolates were from abscesses, peritonitis, decu ...19968667406
the in vitro activity of trospectomycin against anaerobic bacteria.the authors evaluated the activity of trospectomycin, a new aminocyclitol which is characterized by good antibacterial and broad spectrum activity, in comparison with clindamycin and ampicillin on a sample of recent isolates: bacteroides fragilis (15 strains), bacteroides urealyticus (5 strains), bacteroides vulgatus (5 strains), bacteroides spp. (15 strains), prevotella melaninogenica (6 strains), porphyromonas asaccharolytica (7 strains), mobiluncus spp. (3 strains), peptococcus niger (3 strai ...19958667035
effect of co2 on the fermentation capacities of the acetogen peptostreptococcus productus u-1.the fermentative capacities of the acetogenic bacterium peptostreptococcus productus u-1 (atcc 35244) were examined. although acetate was formed from all the substrates tested, additional products were produced in response to co2 limitation. under co2-limited conditions, fructose-dependent growth yielded high levels of lactate as a reduced end product; lactate was also produced under co2-enriched conditions when fructose concentrations were elevated. in the absence of supplemental co2, xylose-de ...19968655492
backbone dynamics of a domain of protein l which binds to immunoglobulin light chains.protein l is a multidomain protein expressed at the surface of some strains of the anaerobic bacterial species peptostreptococcus magnus. the molecule interacts with the variable domain of immunoglobulin (ig) light chains through five repeated homologous domains denoted b1 to b5. the fold of the ig-light-chain-binding b1 domain of protein l (plb1) has been shown to comprise an alpha-helix packed against a four-stranded beta-sheet and therefore resembles the structure of the igg-binding domains o ...19968654399
non-sporing anaerobes in certain surgical group of patients.the magnitude of non-sparing anaerobic (nsa) infections has been defined in the postoperative wounds on colorectum in children (57.1%), general surgery (0%), abdominoperineal and uterocervical operations (11-45%) in gynaecologic and obstetrical cases and perforative peritonitis (25.8-32.3%). children below the 6 months age group bear less risk of acquiring nsa infection. under certain situations, metronidazole combats nsa infections in a better way than other antibacterials. the bacteriology of ...19958648152
clinical significance of dental root canal microflora.previous work by this group has shown that a significant association exists between pain and the presence of either prevotella or peptostreptococcus spp. in dental root canals. the aim of this study was to examine a more extensive series of canals microbiologically, to determine whether any other particular endodontic symptoms or clinical signs showed specific associations with individual bacterial species.19968636492
endodontic pathogens: propagation of infection through patent dentinal tubules in traumatized monkey teeth.periapical pathology indicating endodontic infection, when present in marginal periodontitis-affected teeth, has recently been shown to be an aggravating factor in progression of marginal destruction. this has been associated with patency of dentinal tubules in the tooth cervix, an area normally devoid of cementum following periodontal therapy. these studies are, however hampered by that only circumstantial evidence such as presence of periapical destruction have been applied as criteria of endo ...19958625937
aerobic and anaerobic microbiology of infected hemorrhoids.to study the aerobic and anaerobic microbiology of infected hemorrhoids.19968607502
microbiological features and pathogenesis of peritonsillar abscesses.samples of pus aspirated from 53 peritonsillar abscesses were examined in detail for aerobic and anaerobic bacteria, and the microbiological results correlated with clinical data in 44 cases. in 45 samples (85%) cultures were positive: 7 yielded organisms consistent with an aerobic infection, mainly lancefield group a beta-haemolytic streptococci (5/7), and 38 yielded organisms consistent with an anaerobic infection. the anaerobic infections were usually mixed, but in two cases fusobacterium nec ...19958605900
bacteriology and beta-lactamase activity in acute and chronic maxillary sinusitis.to assess the bacteriology and beta-lactamase enzyme activity in aspirates of 10 acutely and 13 chronically inflamed maxillary sinuses.19968600928
pet findings in a brain abscess associated with a silent atrial septal defect.brain abscesses are classical complications of congenital heart disease (chd) in children and adolescents. this association is rarely observed in adults. we report a 46-year-old man presenting a fronto-parietal abscess associated with an asymptomatic atrial septal defect. positron emission tomography (pet) study revealed high uptake of l-[methyl-11c]methionine ([11c]methionine) and 2-[18f]fluoro-2-deoxy-d-glucose (fdg) around the brain abscess. we suggest (1) to exclude a silent cardiac malforma ...19958599907
antimicrobial and toxic effects of established and potential root canal irrigants.three dilutions of the commonly used irrigant sodium hypochlorite (0.5%, 2.5%, and 5.25%) and the potential irrigants peridex, chlorhexidine gluconate (0.12%), and therasol were tested. alcohol and saline served as controls also. the in vitro portion of this study used four different microorganisms (streptococcus mutans, peptostreptococcus micros, prevotella intermedius, and porphyromonas gingivalis) to determine antimicrobial effect. presterilized 7-mm diameter whatman paper disks soaked with t ...19958596073
phylogenetic analysis of ruminococcus flavefaciens, the type species of the genus ruminococcus, does not support the reclassification of streptococcus hansenii and peptostreptococcus productus as ruminococci.the 16s rrna gene sequence of the type strain of ruminococcus flavefaciens, the type species of the genus ruminococcus, was determined by pcr direct sequencing. a comparative sequence analysis showed that r. flavefaciens is phylogenetically related to a small cluster (cluster iv of collins et al. [m. d. collins, p. a. lawson, a. willems, j. j. cordoba, j. fernandez-garayzabal, p. garcia, j. cai, h. hippe, and j. a. e. farrow, int. j. syst. bacteriol. 44:812-826, 1994]) of organisms which include ...19958590686
oral microbiota in subjects with a weak or strong response in experimental gingivitis.the purpose of the present study was to examine the composition of the oral microbiota in subjects who had previously demonstrated to develop either a weak or strong response to experimental gingivitis. for this study, subjects were selected from a pool of 25 individuals who had participated twice in an experimental gingivitis trial. out of these 25 panellists, 6 subjects were selected who had developed 2x a weak gingival inflammatory response and 7 subjects who had developed 2x a strong gingiva ...19958583022
endodontic pathogens in periodontal disease augmentation.periapical pathology indicating endodontic infection, when present in periodontitis-affected teeth, has recently been shown to be correlated to marginal periodontal breakdown. this has been associated with patency of dentinal tubules in the tooth cervix, an area normally devoid of cementum following periodontal therapy. these studies are, however hampered by that only circumstantial evidence such as presence of periapical destruction have been applied as criteria of endodontic infection. the aim ...19958583015
identification and discrimination of oral asaccharolytic eubacterium spp. by pyrolysis mass spectrometry and artificial neural networks.curie-point pyrolysis mass spectra were obtained from 29 oral asaccharolytic eubacterium strains and 6 abscess isolates previously identified as peptostreptococcus heliotrinreducens. pyrolysis mass spectrometry (pyms) with cluster analysis was able to clarify the taxonomic position of this group of organisms. artificial neural networks (anns) were then trained by supervised learning (with the back-propagation algorithm) to recognize the strains from their pyrolysis mass spectra; all eubacterium ...19968574131
microbiology of mediastinitis.to study the microbiologic and clinical characteristics of patients with mediastinitis.19968572845
a case of anaerobic pericarditis. 19958562770
treatment of peptostreptococcus micros endocarditis with teicoplanin. 19958562764
aerobic and anaerobic microbiology of superficial suppurative thrombophlebitis.to study the aerobic and anaerobic microbiologic characteristics of superficial suppurative thrombophlebitis.19968546586
competition for peptides and amino acids among periodontal bacteria.we recently studied the utilization of glutathione (l-gamma-glutamyl-l-cysteinylglycine), l-cysteinylglycine and l-cysteine by anaerobic bacteria. the rate of hydrogen sulfide formation from these compounds was determined and it was concluded that peptostreptococcus micros and fusobacterium nucleatum subsp. nucleatum had an active transport of small peptides. in the present study it is shown that methyl mercaptan formation from l-methionine and l-methionyl-containing peptides can also be used to ...19958544102
salivary levels of suspected periodontal pathogens in relation to periodontal status and treatment.the primary ecological niche for suspected periodontal pathogens seems to be the subgingival area, even though periodontal pathogens are also frequently recovered from saliva. the interrelationship of different periodontal conditions and the salivary levels of suspected periodontal pathogens is not known. in the present study, salivary levels of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, and peptostreptococcus micros were determin ...19958530742
structures of bacterial immunoglobulin-binding domains and their complexes with immunoglobulins.three-dimensional structures are now available for several immunoglobulin binding domains from bacterial proteins a, g, and l. x-ray diffraction and nmr experiments on complexes of these domains with portions of immunoglobulins have revealed common structural themes used in these interactions. these data expand our understanding of structure/function relationships in these molecular recognition processes and provide the basis for rational design of artificial immunoglobulin-binding molecules.19958528763
bioactivation of cysteine conjugates of 1-nitropyrene oxides by cysteine conjugate beta-lyase purified from peptostreptococcus magnus.to determine the role of cysteine conjugate beta-lyase (beta-lyase) in the metabolism of mutagenic nitropolycyclic aromatic hydrocarbons, we determined the effect of beta-lyase on the mutagenicities and dna binding of cysteine conjugates of 4,5-epoxy-4,5-dihydro-1-nitropyrene (1-np 4,5-oxide) and 9,10-epoxy-9,10-dihydro-1-nitropyrene (1-np 9,10-oxide), which are detoxified metabolites of the mutagenic compound 1-nitropyrene. we purified beta-lyase from peptostreptococcus magnus gai0663, since p. ...19958526486
black-pigmented gram-negative anaerobes in endodontic infections.necrotic dental root canal infections are polymicrobial infections dominated by anaerobic bacteria. the number of different species in one canal is usually low, approx. 4-7 species. the species isolated most frequently belong to the genera prevotella, porphyromonas, fusobacterium, peptostreptococcus, eubacterium and streptococcus. the frequency of isolation of black-pigmented gram-negative anaerobes in endodontic infections varies from 25% to > 50%. pr. intermedia is the most commonly found pigm ...19938518758
in vitro activity of fk037, a new parenteral cephalosporin, against anaerobic bacteria.the activity of fk037, a new parenteral cephalosporin, was compared with those of cefpirome, ceftazidime, and flomoxef against 322 recent clinical isolates of anaerobic bacteria. a fastidious facultative anaerobe, gardnerella vaginalis, was also studied. fk037 inhibited 90% of isolates of peptostreptococcus anaerobius, peptostreptococcus asaccharolyticus, clostridium perfringens, mobiluncus spp., g. vaginalis, and porphyromonas gingivalis at < or = 0.78 micrograms/ml. the mics of fk037 for 50 an ...19938517721
infected epidermoid cyst of the sphenoid bone.an infected epidermoid cyst presented on ct as a primarily dense, sclerotic expansile lesion in the greater wing of the sphenoid bone. presumably, infection was responsible for the atypical appearance.19938517372
[bacteriological and clinical studies in 23 cases of thoracic empyema--the role of oral streptococci and anaerobes].the bacteriology of empyema fluid and the clinical background of 23 cases from july 1987 through july 1992 were studied. nineteen cases were male and 4 female, with a mean age of 59.6 years (range; 33 to 84 y.o.). there were 15 cases of community-acquired infection and 8 of nosocomial infection. acute pneumonia and/or lung abscess developed into empyema in 19 cases. sixteen cases had associated predisposing conditions, such as diabetes, chronic bronchitis, disorders with dysphagia, and excess al ...19938515622
peptostreptococcus micros has a uniquely high capacity to form hydrogen sulfide from glutathione.there are high amounts of hydrogen sulfide in deep periodontal pockets. this volatile sulfur compound may be formed from l-cysteine, but only low levels of this amino acid can be expected to be present in periodontal pockets. glutathione, l-gamma-glutamyl-l-cysteinylglycine, is in high concentration in most tissue cells, and this tripeptide may be more readily available as a source of hydrogen sulfide formation in the pockets. the ability of 37 different species of oral bacteria to utilize gluta ...19938510983
antibacterial activity of oral antibiotics against anaerobic bacteria.the spectrum and the antibacterial susceptibility of anaerobic bacteria to oral antibiotics isolated from clinical specimens was assessed in two different centres, the first receiving specimens from university departments and the second from general practitioners and small hospitals. susceptibility was studied with a microtiter ready-to-use panel system, using the manufacturer's modified wilkins-chalgren's broth as the test medium for the following antibiotics: ampicillin, ampicillin+sulbactam, ...19938508686
do infected inguinal lymph nodes increase the incidence of postoperative groin wound infection?risk factors for postoperative wound infection in patients undergoing vascular surgery may include age, comorbid conditions, wound classification, use of prosthetic grafts, and repeat operations. groin incisions, in particular, pose substantial risk during placement of prosthetic grafts.19938505786
spinal epidural abscess: unusual ct and microbiologic findings.the incidence of spinal epidural abscess is increasing. its expeditious diagnosis is essential if treatment is to be effective. the authors present a case in which both the computed tomography appearance and the microbiologic observations were unusual.19938504337
[recent bacteriologic data: from physiopathology to treatment].bacterial vaginosis is characterized by a change in the vaginal ecosystem in which lactobacillus spp, the dominant members of the normal flora, are replaced by an association of various bacterial species including gardnerella vaginalis, anaerobes (bacteroides spp, prevotella spp, porphyromonas spp, peptostreptococcus spp and mobiluncus spp) and mycoplasma hominis. the reasons for this imbalance are unknown, although the loss of lactobacilli that produce hydrogen peroxide (which is toxic for g. v ...19938484089
on the interaction between protein l and immunoglobulins of various mammalian species.protein l, a cell wall molecule of certain strains of the anaerobic bacterial species peptostreptococcus magnus, shows high affinity for human immunoglobulin (ig) light chains. in the present study protein l was tested against a panel of human myeloma proteins of the igg, igm, iga and ige classes, and strong binding was seen with antibodies carrying kappa light chains. a high degree of specificity for ig was demonstrated in binding experiments with human plasma proteins. apart from human ig, str ...19938469922
intrapleural streptokinase in experimental empyema.intrapleural streptokinase has been used in multiloculated empyemas to enhance pleural space drainage, presumably by causing fibrinolysis of the interlocular septae. we evaluated the efficacy and safety of daily administration of 10,000 u intrapleural streptokinase or equal volumes of saline to enhance resolution of experimental empyema in the rabbit pleural space. seventy-two hours after intrapleural turpentine, 10(8) colony-forming units each of escherichia coli, peptostreptococcus anaerobius, ...19938466134
microbial composition and pattern of antibiotic resistance in subgingival microbial samples from patients with refractory periodontitis.a series of 993 subgingival microbial samples sent to a diagnostic microbiology laboratory included 196 samples that could be identified as compatible with a clinical diagnosis of refractory or recurrent periodontitis. in descending order of prevalence the associated microbiota included bacteroides forsythus (84%), spirochetes (83%), motile rods (76%), fusobacterium species (68%), porphyromonas gingivalis (63%), campylobacter rectus (47%), capnocytophaga species (38%), prevotella intermedia (23% ...19938463936
proton nuclear magnetic resonance sequential assignments and secondary structure of an immunoglobulin light chain-binding domain of protein l.the 1h nmr assignments have been made for the immunoglobulin (ig) light chain-binding b1 domain of protein l from peptostreptococcus magnus. the secondary structure elements and the global folding pattern were determined from nuclear overhauser effects, backbone coupling constants, and slowly exchanging amide protons. the b1 domain was found to be folded into a globular unit of 61 amino acid residues, preceded by a 15 amino acid long disordered n-terminus. the folded portion of the molecule cont ...19938461301
in vitro and in vivo antibacterial activities of t-3761, a new quinolone derivative.t-3761, a new quinolone derivative, showed broad and potent antibacterial activity. its mics for 90% of the strains tested were 0.20 to 100 micrograms/ml against gram-positive bacteria, including members of the genera staphylococcus, streptococcus, and enterococcus; 0.025 to 3.13 micrograms/ml against gram-negative bacteria, including members of the family enterobacteriaceae and the genus haemophilus; 0.05 to 50 micrograms/ml against glucose nonfermenters, including members of the genera pseudom ...19938460909
a review of commonly prescribed oral antibiotics in general dentistry.the microflora associated with odontogenic infections are typically mixed and of indigenous origin. streptococcus, peptostreptococcus, peptococcus, fusobacterium, bacteroides, and actinomyces species are the principle microflora isolated from these infections. penicillin v (phenoxymethyl penicillin) remains the antimicrobial of choice for the initial empirical treatment of odontogenic infections. this agent is safe, highly effective and inexpensive. amoxicillin has little indication for the rout ...19938453530
metabolism of safflor yellow b by human intestinal bacteria. 19938450320
contemporary management of deep infections of the neck.odontogenic infections rarely lead to involvement of the lateral and retropharyngeal spaces. when this does occur, the microbiology of the infection is similar to the typical odontogenic infection, ie, streptococcus and oral anaerobes including peptostreptococcus, bacteroides, and fusobacterium. there is an increased incidence of fusobacterium seen in the more severe infections, as well as a higher incidence of streptococcus milleri. many patients who have deep cervical infections also have some ...19938445462
phylogeny of the ammonia-producing ruminal bacteria peptostreptococcus anaerobius, clostridium sticklandii, and clostridium aminophilum sp. nov.in previous studies, gram-positive bacteria which grew rapidly with peptides or an amino acid as the sole energy source were isolated from bovine rumina. three isolates, strains c, ft (t = type strain), and sr, were considered to be ecologically important since they produced up to 20-fold more ammonia than other ammonia-producing ruminal bacteria. on the basis of phenotypic criteria, the taxonomic position of these new isolates was uncertain. in this study, the 16s rrna sequences of these isolat ...19938427801
sub-gingival microflora in macaca mulatta species of rhesus monkey.the macaca mulatta species of rhesus monkey is one of several non-human primate (nhp) models for periodontal disease. this report presents the bacteriology of the gingival sulci in m. mulatta monkeys. three sub-gingival sites (maxillary right central incisor, the disto-buccal of the mandibular left second molar and mesio-buccal of the mandibular right second molar) of 9 monkeys were evaluated clinically before scaling and 7 days after scaling. plaque samples were obtained from sub-gingival sites ...19938426283
role of anaerobic bacteria in liver abscesses in children.aspirates from pyogenic liver abscesses obtained from 14 children were cultured for aerobic and anaerobic bacteria. of 29 organisms that were recovered, 17 were anaerobic and 12 were aerobic or facultative. the predominant organisms were peptostreptococcus spp. (5 isolates). bacteroides fragilis group (4), fusobacterium spp. (3) and staphylococcus aureus (4). aerobic or facultative bacteria only were recovered in five cases, anaerobic bacteria only in four and mixed aerobic and anaerobic bacteri ...19938414802
case records of the massachusetts general hospital. weekly clinicopathological exercises. case 43-1993. a 71-year-old woman with confusion, hemianopia, and an occipital mass. 19938413415
the influence of race and gender on periodontal microflora.previous studies have demonstrated that demographic characteristics of subject populations influence both the incidence of periodontal diseases and various aspects of host responses to periodontal bacteria. in this study we analyzed the components of the subgingival microflora from individuals with adult periodontitis, early onset periodontitis, gingivitis, and periodontal health as a function of gender and race (black and white). clinical categories were analyzed individually so that there were ...19938387107
purification of antibodies using protein l-binding framework structures in the light chain variable domain.protein l from the bacterial species peptostreptococcus magnus binds specifically to the variable domain of ig light chains, without interfering with the antigen-binding site. in this work a genetically engineered fragment of protein l, including four of the repeated ig-binding repeat units, was employed for the purification of ig from various sources. thus, igg, igm, and iga were purified from human and mouse serum in a single step using protein l-sepharose affinity chromatography. moreover, hu ...19938360508
lung abscess: analysis of 66 cases from 1979 to 1991.sixty-six patients with bacterial lung abscess were treated between 1979 and 1991 in our hospital. among these patients, death occurred in one of the 42 cases of community-acquired infection (mortality rate: 2.4%) and in 16 of the 24 cases of nosocomial infection (mortality rate: 66.7%). of all 66 cases, 55 were culture-positive, and the etiologic agents isolated from 24 of the culture-positive cases were found to be anaerobic bacteria. the most common aerobes isolated from the foci were identif ...19938358116
the microbiology of bacterial vaginosis.a group of microorganisms are present concurrently in high concentrations in the vaginas of women with bacterial vaginosis. the major members of the group are gardnerella vaginalis, anaerobic gram-negative rods belonging to the genera prevotella, porphyromonas and bacteroides, peptostreptococcus species, mycoplasma hominis, ureaplasma urealyticum, and often mobiluncus species. facultative species of lactobacillus are present in lower concentrations and are less prevalent in bacterial vaginosis t ...19938357043
l-serine and l-threonine dehydratase from clostridium propionicum. two enzymes with different prosthetic groups.l-serine dehydratase from the gram-positive bacterium peptostreptococcus asaccharolyticus is novel in the group of enzymes deaminating 2-hydroxyamino acids in that it is an iron-sulfur protein and lacks pyridoxal phosphate [grabowski, r. and buckel, w. (1991) eur. j. biochem. 199, 89-94]. it was proposed that this type of l-serine dehydratase is widespread among bacteria but has escaped intensive characterization due to its oxygen lability. here, we present evidence that another gram-positive ba ...19938344301
[a case of empty sella syndrome and pan-hypopituitarism associated with acute suppurative paranasal sinusitis]. 19938340667
[actinomycosis infection of the cerebellum].the case is presented of a 37-year-old male with a cerebellar abscess due to actinomyces israelii. the patient was admitted after dental surgery because of fever and symptoms indicative of raised, intracranial pressure. no abnormalities were detected in peripheral blood and cerebrospinal fluid. ct scan revealed a space-occupying lesion in the left cerebellar hemisphere with perifocal oedema and occlusive hydrocephalus. after emergency external ventriculostomy complete removal of the mass was ach ...19938333207
oral and dental infections with anaerobic bacteria: clinical features, predominant pathogens, and treatment.microbial populations colonizing the teeth are a major source of pathogens responsible for oral and dental infections, including periodontal diseases, gingivitis, pericoronitis, endodontitis, peri-implantitis, and postextraction infections. each entity has distinct clinical and microbial features. bacterial species associated with oral infections include actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, bacteroides forsythus, campylobacter rectus, eubacterium ...19938324136
peritonsillar abscess, retropharyngeal abscess, mediastinitis, and nonclostridial anaerobic myonecrosis: a case report.peritonsillar abscess is a potentially life-threatening complication of acute tonsillitis. on occasion, peritonsillar abscess can extend to neck spaces and/or to the mediastinum. we describe a case of a patient with a peritonsillar abscess that extended to the neck, producing bilateral retropharyngeal abscesses and myonecrosis of the strap muscles. culture of a specimen of the necrotic muscle yielded prevotella intermedia, prevotella buccae, lactobacillus catenaforme, another lactobacillus speci ...19938324135
bacteriologic findings in peritonsillar abscesses in young adults.aspirated pus samples from 124 patients with peritonsillar abscess were cultured quantitatively for aerobes and anaerobes. a total of 98% of the samples yielded bacteria. of the 550 isolates obtained (mean, 4.4 per patient), 143 were aerobes (representing 16 species or groups) and 407 were anaerobes (representing 40 species or groups). aerobes were isolated from 86% of patients-alone in 20 cases and together with anaerobes in 87. the most common aerobic isolates were streptococcus pyogenes (isol ...19938324134
the normal vaginal flora, h2o2-producing lactobacilli, and bacterial vaginosis in pregnant women.in this study of the vaginal flora of 171 pregnant women in labor at term, the flora was categorized as normal (lactobacillus predominant), intermediate, or representative of bacterial vaginosis (bv) on the basis of a vaginal smear. bv was diagnosed in 39 women (23%); the vaginal flora was classified as normal in 50% of cases and as intermediate in 27%. h2o2-producing lactobacilli were recovered from 5% of women with bv, 37% of those with an intermediate flora, and 61% of those with a normal flo ...19938324131
bacteriology of anaerobic pleuropulmonary infections: preliminary report.a retrospective bacteriologic study of anaerobic pleuropulmonary infections diagnosed at the wadsworth veterans affairs medical center between 1976 and 1991 was performed. there were 116 specimens from 110 patients. available strains were reexamined using the latest tests and taxonomic schemes. pleural fluid was believed to provide the most reliable specimen; cultures yielded an average of 3.0 anaerobes and 0.6 nonanaerobes per specimen. the most commonly encountered anaerobes were pigmented pre ...19938324128
anaerobic bacterial infections of the lung and pleural space.in 193 reviewed cases of pleuropulmonary infections involving anaerobic bacteria, the predominant clinical syndromes were aspiration pneumonia, lung abscess, and empyema. most patients had an indolent infection with tissue necrosis (abscess or bronchopulmonary fistula) at initial presentation. nevertheless, many had a more fulminant disease course resembling that of pneumococcal pneumonia. transtracheal aspiration was the procedure most commonly used to obtain uncontaminated specimens for anaero ...19938324127
recent taxonomic changes for anaerobic gram-positive and selected gram-negative organisms.the strains formerly classified as streptococcus anginosus or "streptococcus milleri" have now been recognized as three distinct species, streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. streptococcus morbillorum has been transferred into the genus gemella. four new species within the genus peptostreptococcus were recently named. a recent addition to the genus clostridium is clostridium argentinense, which includes clostridium botulinum type g. two new species ...19938324113
[studies on the locality and the function of spirosome in bacterial cells: relationship between the production of spirosome and anaerobic glycolysis].the locality and the function of spirosome were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and electron microscopy in peptostreptococcus productus, clostridium sp. hd-17, lactobacillus fermentum, eubacterium aerofaciens and escherichia cali b. when the bacterial cells in phosphate buffered saline were disrupted by sonication and fractionated by differential centrifugation, the spirosome protein was found in the cytoplasmic fraction and not in the fraction of ...19938315808
[clinical study of anaerobic respiratory infection].this clinical study involved 35 cases, anaerobic bacteria were detected by tta (transtracheal aspiration) or percutaneous lung aspiration, or pleural puncture. these cases were treated over the last 8 years in our department. there were 9 empyema, 9 pneumonitis, 5 lung abscess, 1 necrotizing pneumonia and 11 chronic lower airway infection. in 13 cases (37%), anaerobic bacteria alone were detected, whereas both anaerobic and aerobic bacteria were observed in the other 22 cases (63%). of all bacte ...19938315301
[multiple hepatic-splenic abscesses. a favorable result with antibiotic treatment].the authors report a case of multiple and spleen pyogenic abscesses caused by peptostreptococcus spp., in a patient who recently underwent laparotomy due to duodenal perforation. he was successfully treated by means of intensive and extended antibiotherapy. we would like to stress the importance of an early and etiological diagnosis performed by percutaneous puncture and bacteriological study, in order to start a selective antibiotherapy. other therapeutical possibilities are discussed.19938292440
piperacillin and tazobactam versus clindamycin and gentamicin in the treatment of hospitalized women with pelvic infection. the piperacillin/tazobactam study group.to compare the efficacy and safety of a combination of piperacillin and tazobactam with that of clindamycin and gentamicin in the treatment of hospitalized women with infections of the upper genital tract.19948290195
in vitro activity of bay y3118 against anaerobic bacteria.the antimicrobial activity of a new quinolone, bay y3118, was determined against 326 strains of anaerobic bacteria and compared with the activities of ampicillin-sulbactam, cefotetan, clindamycin, imipenem, metronidazole, and sparfloxacin. the national committee for clinical laboratory standards-approved wadsworth agar dilution technique with brucella-laked blood agar was used throughout the study. breakpoints used to determine the percent susceptible were 2 micrograms/ml for bay y3118 and sparf ...19938285647
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