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[nurse's role in the oral hygiene of her patients]. 20154492959
continuous elevation by spica cast in treatment of reflex sympathetic dystrophy. 20154452701
the stimulatory effect on insulin secretion in long-term tolbutamide treatment. immunological determinations of urinary- and serum insulin. 20154678208
hospital physician relations. 20154523826
[diagnosis of isolated nerve lesions (author's transl)]. 20154372027
problems of the single-parent family unit.case reports on single-parents families demonstrate some unique problems with which such a family unit must cope. single mothers frequently present children to the family physician, pediatrician or child psychiatrist with specific symptom complaints. there exists a need to recognize that these symptoms may reflect the special problems of the single-parent family or unresolved issues which led to the formation of the unit.to meet the needs of these parents the physician must explore the specific ...20154653933
comparison of the pge1 and catecholamines influence on the active transport of sodium. 20154365176
tuberous sclerosis and schizophrenia. 20154648445
biomedical librarians in a patient care setting at the university of missouri-kansas city school of medicine.the biomedical librarian has been placed in a patient care setting working in the specific environment of a six-year medical school guided by the docent team concept (docent is defined as a clinician-scholar). within this framework the specifically qualified clinical medical librarians function within the docent unit. three dissemination techniques or systems-latch, current references, and latest topics-are discussed. note is made of the clinical medical librarian's role in teaching, demand sear ...20154462680
"once more into the breach"--to the defense of health education. 20154490491
fermentation characteristics and lactic acid bacteria succession of total mixed ration silages formulated with peach pomace.the objective of this study was to assess the use of peach pomace in total mixed ration (tmr) silages and clarify the differences in aerobic stability between tmr and tmr silages caused by lactic acid bacteria (lab). the tmr were prepared using peach pomace, alfalfa hay or leymus chinensis hay, maize meal, soybean meal, cotton meal, limestone, a vitamin-mineral supplement, and salt in a ratio of 6.0:34.0:44.4:7.0:5.0:2.5:1.0:0.1 on a dry matter (dm) basis. fermentation quality, microbial composi ...201525656205
application of recombinant pediococcus acidilactici bd16 (fcs⁺/ech⁺) in malolactic fermentation.this study was conducted to enhance flavor characteristics of wine by malolactic fermentation using recombinant pediococcus acidilactici bd16 (fcs (+)/ech (+)) encoding synthetic genes of feruloyl-coa synthetase and enoyl-coa hydratase. after malolactic fermentation, wine phenolics were characterized using lcms-esi technique and a significant improvement in the antioxidant activity and flavor characteristics of wine was observed due to increased concentration of cinnamic acid derivatives. this p ...201525650327
biochemical, kinetic, and in silico characterization of ding protein purified from probiotic lactic acid bacteria pediococcus acidilactici ncdc 252.ding proteins are intriguing proteins characterized by conserved n-terminal sequence. in spite of unusually high sequence conservation even between distantly related species, ding proteins exhibit outstanding functional diversity. an extracellular caseinolytic alkaline enzyme was purified to homogeneity from a probiotic lactic acid bacteria pediococcus acidilactici ncdc 252 using a simple procedure involving ammonium sulphate precipitation and gel filtration chromatography. this was purified 45. ...201525367285
effects of probiotics pediococcus acidilactici strain ma18/5m and saccharomyces cerevisiae subsp. boulardii strain sb-cncm i-1079 on fecal and intestinal microbiota of nursing and weanling piglets.in this study, the influence of (pa) and subsp. (scb) on fecal and intestinal microbiota of piglets during lactation and after weaning was monitored. forty sows and their litters were used and allocated to the following dietary treatments: 1) pa, 2) scb, 3) a mixture of the 2 probiotics (pa+scb), 4) antibiotics (atb), and 5) control (ctrl). four weeks before parturition, probiotic-treated sows started receiving a daily probiotic dose of at least 2.5 × 10 cfu mixed in 500 g of feed until the end ...201526641051
antioxidative and anticanceric activities of magnolia (magnolia denudata) flower petal extract fermented by pediococcus acidilactici kccm 11614.in this study, the effects of magnolia (magnolia (m.) denudata) extract fermentation in increasing the extract's antioxidative and anticancer activities were investigated. magnolia was fermented by pediococcus acidilactici kccm 11614. the total phenolic content was determined by the folin-ciocalteu's method and the antioxidative effects by 1,1-diphenyl-2-picrylhydrazy (dpph) and ferric reducing ability of plasma (frap) assay. anticancer activity against cancer and normal cells was determined usi ...201526151113
analysis of cell growth dynamics of pediococcus acidilactici in the presence of inulin in an optimized microenvironment.the present investigation deals with the optimization of cell growth rate of the candidate probiotic pediococcus acidilactici in the presence of the specific prebiotic inulin. three independent variables viz. concentration of inulin, concentration of glucose and ph have been selected for optimization study using response surface methodology. theoretical analysis indicates that the maximum cell growth rate occurs at ph 7, 20 g/dm(3) concentration of inulin and 20 g/dm(3) concentration of glucose. ...201526159350
in vitro determination of volatile compound development during starter culture-controlled fermentation of cucurbitaceae cotyledons.the effects of lactobacillus plantarum ufla ch3, pediococcus acidilactici ufla bffcx 27.1, and torulaspora delbrueckii ufla fft2.4 inoculation on the volatile compound profile of fermentation of cucumeropsis mannii cotyledons were investigated. different microbial associations were used as starters. all associations displayed the ability to ferment the cotyledons as judged by lowering the ph from 6.4 to 4.4-5 within 24h and increasing organic acids such as lactate and acetate. the population of ...201525306300
combined moist airtight storage and feed fermentation of barley by the yeast wickerhamomyces anomalus and a lactic acid bacteria consortium.this study combined moist airtight storage of moist grain with pig feed fermentation. starter cultures with the potential to facilitate both technologies were added to airtight stored moist crimped cereal grain, and the impact on storage microflora and the quality of feed fermentations generated from the grain was investigated. four treatments were compared: three based on moist barley, either un-inoculated (m), inoculated with wickerhamomyces anomalus (w), or inoculated with w. anomalus and lab ...201525954295
pcr-dgge assessment of the bacterial diversity in spanish-style green table-olive fermentations.the bacterial ecology associated to spanish-style green olive fermentations has been studied, attending to its dynamics along the time and its distribution, by a culture-independent approach based on pcr-dgge. forty-three 10-tonne fermenters were selected from the fermentation yards (patios) of two large table-olive manufacturing companies in southern spain. the fermenting brines of 20 of these fermenters were previously analyzed through culture-dependent methods, allowing comparisons of both me ...201525886017
antilisterial activity of bacteriocinogenic pediococcus acidilactici ha6111-2 and lactobacillus plantarum esb 202 grown under ph and osmotic stress conditions.bacteriocin producing lactic acid bacteria (lab) cultures can be used as biopreservatives in fermented food products; thus the food industry is interested in stable cultures that produce bacteriocins consistently. inhibtion of listeria spp. by bacteriocinogenic pediococcus acidilactici and lactobacillus plantarum (both isolated from fermented meats) was investigated under conditions of stress induced by low ph and high salt concentrations. listeria monocytogenes serogroup iib (from cheese), l. m ...201525790998
microbial communities involved in kaşar cheese ripening.the microbiota of non-starter lactic acid bacteria (nslab) and their concomitant community dynamics during cheese ripening were investigated for traditional turkish kaşar cheeses made from raw cows' milk. five batches of 15 kaşar cheeses produced in different dairy plants located in kars were analysed during their whole ripening phase up to 180 days. lactobacilli and lactococci were determined as the prevailing microbial groups. the molecular classification and identification of 594 lab isolates ...201525475332
protective role of probiotic lactic acid bacteria against dietary fumonisin b1-induced toxicity and dna-fragmentation in sprague-dawley rats.the genus fusarium, especially f. verticillioides and f. proliferatum, has been found in several agricultural products worldwide, especially in maize. regardless the occurrence of symptoms, the presence of fusarium in maize constitutes an imminent risk due to its ability to produce fumonisins, mycotoxins with proven carcinogenic effect on rats, swine, and equines and already classified as possible carcinogens to humans. the toxicity of incremental levels of fumonisin b1 (fb1), that is, 50, 100, ...201525036875
in-vitro antioxidant and antibacterial properties of fermentatively and enzymatically prepared chicken liver protein hydrolysates.protein hydrolysates were prepared from chicken liver using fermentation and enzymatic hydrolysis. the lactic acid bacteria pediococcus acidilactici ncim5368 was employed in the fermentation process and a commercial protease (alcalase® 2.5) was used in enzymatic hydrolysis. chicken liver hydrolysates prepared by fermentation (fclh) and enzymatic hydrolysis (eclh) revealed appreciable amounts of protein [55.85 and 61.34 %; on dry weight basis, respectively]. fermentation and enzymatic hydrolysis ...201526604378
influence of supplemented diet with pediococcus acidilactici on non-specific immunity and stress indicators in green terror (aequidens rivulatus) during hypoxia.green terror fish were fed for two months with three types of feed including: control diet (c), diet containing 2% fish oil (o) and diet containing fish oil supplemented with 0.1% pediococcus acidilactici bacteria (pa). at the end of the feeding period, 50 fish of different groups (n = 3) with an average weight of 4.28 g were transferred to 9 tank. hypoxia tests were set out by sampling at three times including before hypoxia (bh) initiation, hypoxia stress spot (h) and starting mortality (sm). ...201525891275
growth, immune responses and intestinal morphology of rainbow trout (oncorhynchus mykiss) supplemented with commercial probiotics.the influence of two commercial probiotics on the growth, innate immune parameters and intestinal morphology of rainbow trout (oncorhynchus mykiss) juveniles (initial weight: 16.4 ± 0.4 g) was evaluated. two probiotic types: a, multi-species (bacillus sp., pedicoccus sp., enterococcus sp., lactobacillus sp.) and b, mono-species (pediococcus acidilactici) were tested at two levels each (a1: 1.5 g kg(-1), 8.6 × 10(5) cfu g(-1); a2: 3 g kg(-1), 1.6 × 10(6) cfu g(-1); b1: 0.1 g kg(-1), 2.6 × 10(4) c ...201525865055
expression, purification, and characterization of a bifunctional 99-kda peptidoglycan hydrolase from pediococcus acidilactici atcc 8042.pediococcus acidilactici atcc 8042 is a lactic acid bacteria that inhibits pathogenic microorganisms such as staphylococcus aureus through the production of two proteins with lytic activity, one of 110 kda and the other of 99 kda. the 99-kda one has high homology to a putative peptidoglycan hydrolase (pgh) enzyme reported in the genome of p. acidilactici 7_4, where two different lytic domains have been identified but not characterized. the aim of this work was the biochemical characterization of ...201525940238
high titer l-lactic acid production from corn stover with minimum wastewater generation and techno-economic evaluation based on aspen plus modeling.technological potentials of l-lactic acid production from corn stover feedstock were investigated by experimental and techno-economic studies. an optimal performance with 104.5 g/l in l-lactic acid titer and 71.5% in overall yield from cellulose in corn stover to l-lactic acid using an engineered pediococcus acidilactici strain were obtained by overcoming several technical barriers. a rigorous aspen plus model for l-lactic acid production starting from dry dilute acid pretreated and biodetoxifie ...201526454367
a microbial feed additive abates intestinal inflammation in atlantic salmon.the efficacy of a microbial feed additive (bactocell(®)) in countering intestinal inflammation in atlantic salmon was examined in this study. fish were fed either the additive-coated feed (probiotic) or feed without it (control). after an initial 3-week feeding, an inflammatory condition was induced by anally intubating all the fish with oxazolone. the fish were offered the feeds for 3 more weeks. distal intestine from the groups was obtained at 4 h, 24 h, and 3 weeks, after oxazolone treatment. ...201526347738
influence of sub-lethal stresses on the survival of lactic acid bacteria after spray-drying in orange juice.the demand for new functional non-dairy based products makes the production of a probiotic orange juice powder an encouraging challenge. however, during drying process and storage, loss of viability of the dried probiotic cultures can occur, since the cells are exposed to various stresses. the influence of sub-lethal conditions of temperature, acidic ph and hydrogen peroxide on the viability of pediococcus acidilactici ha-6111-2 and lactobacillus plantarum 299v during spray drying in orange juic ...201526338119
intravaginally administered lactic acid bacteria expedited uterine involution and modulated hormonal profiles of transition dairy cows.the objective of this investigation was to evaluate whether intravaginal infusion of lactic acid bacteria (lab) around parturition could expedite involution rate of the uterus and improve reproductive performance of postpartum dairy cows. one hundred pregnant holstein dairy cows were assigned to 1 of 3 experimental groups: (1) 1 dose of lab in wk -2 and -1 and 1 dose of carrier in wk 1 relative to the expected day of parturition (trt1); (2) 1 dose of lab in wk -2, -1, and 1 (trt2); and (3) 1 dos ...201526188583
isolation and taxonomic identity of bacteriocin-producing lactic acid bacteria from retail foods and animal sources.bacteriocin-producing (bac⁺) lactic acid bacteria (lab) were isolated from a variety of food products and animal sources. samples were enriched in de man, rogosa, and sharpe (mrs) lactocilli broth and plated onto mrs agar plates using a "sandwich overlay" technique. inhibitory activity was detected by the "deferred antagonism" indicator overlay method using listeria monocytogenes as the primary indicator organism. antimicrobial activity against l. monocytogenes was detected by 41 isolates obtain ...201527682080
the use of the replication region of plasmid prs7 from oenococcus oeni as a putative tool to generate cloning vectors for lactic acid bacteria.a chimeric plasmid, prs7rep (6.1 kb), was constructed using the replication region of prs7, a large plasmid from oenococcus oeni, and pem64, a plasmid derived from pij2925 and containing a gene for resistance to chloramphenicol. prs7rep is a shuttle vector that replicates in escherichia coli using its pij2925 component and in lactic acid bacteria (lab) using the replication region of prs7. high levels of transformants per µg of dna were obtained by electroporation of prs7rep into pediococcus aci ...201525479060
modulation of innate immune response, mucosal parameters and disease resistance in rainbow trout (oncorhynchus mykiss) upon synbiotic feeding.the present study investigates the effects of dietary supplements of galactooligosaccharides (gos), pediococcus acidilactici and p. acidilactici + gos on innate immune response, skin mucus as well as disease resistance of rainbow trout (oncorhynchus mykiss) fingerlings (15.04 ± 0.52 g). after 8 weeks of feeding, several innate immune (lysozyme, alternative complement and respiratory burst activities) and skin mucus parameters (bactericidal activity against streptococcus faecium, streptococcus in ...201525827628
modulation of the intestinal microbiota and morphology of tilapia, oreochromis niloticus, following the application of a multi-species probiotic.the intestinal microbiota and morphology of tilapia (oreochromis niloticus) were investigated after the application of a multi-species probiotic containing lactobacillus reuteri, bacillus subtilis, enterococcus faecium and pediococcus acidilactici (aquastar(®) growout). tilapia (55.03 ± 0.44 g) were fed either a control diet or a probiotic diet (control diet supplemented with aquastar(®) growout at 5 g kg(-1)). after four and eight weeks, culture-dependent analysis showed higher levels of lactic ...201526115752
effect of dietary incorporation of a multi-strain probiotic on growth performance and health status in rainbow trout (oncorhynchus mykiss).dietary supplementation with a multi-strain probiotic containing bacillus subtilis, enterococcus faecium, pediococcus acidilactici and lactobacillus reuteri has been examined for its effects on growth performance, intestinal microbiota, non-specific immune response and antioxidant status of rainbow trout. three groups of sub-adult trout were stocked into experimental tanks. a commercial diet was used as control, while the other two groups received diets supplemented with the multi-strain probiot ...201525411048
a microplate growth inhibition assay for screening bacteriocins against listeria monocytogenes to differentiate their mode-of-action.lactic acid bacteria (lab) have historically been used in food fermentations to preserve foods and are generally-recognized-as-safe (gras) by the fda for use as food ingredients. in addition to lactic acid; some strains also produce bacteriocins that have been proposed for use as food preservatives. in this study we examined the inhibition of listeria monocytogenes 39-2 by neutralized and non-neutralized bacteriocin preparations (bac+ preps) produced by lactobacillus curvatus fs47; lb. curvatus ...201526111195
the influence of the wooden equipment employed for cheese manufacture on the characteristics of a traditional stretched cheese during ripening.the influence of the wooden equipment used for the traditional cheese manufacturing from raw milk was evaluated on the variations of chemico-physical characteristics and microbial populations during the ripening of caciocavallo palermitano cheese. milk from two farms (a, extensive; b, intensive) was processed in traditional and standard conditions. chemical and physical traits of cheeses were affected by the farming system and the cheese making technology, and changed during ripening. content in ...201525475270
evaluating pediococcus acidilactici and enterococcus faecium nrrl b-2354 as thermal surrogate microorganisms for salmonella for in-plant validation studies of low-moisture pet food products.pediococcus acidilactici atcc 8042 and enterococcus faecium nrrl b-2354 were investigated as potential surrogates for salmonella serovars using thermal death time kinetics in products such as dry pet foods. the d-values of p. acidilactici atcc 8042, e. faecium nrrl b-2354, and a cocktail of seven salmonella serovars associated with low-moisture products were determined in a preservative-free dry pet food product at moisture levels of 9.1, 17.9, and 27.0% and heated between 76.7 and 87.8°c. the d ...201525951387
impact of cell-free supernatant of lactic acid bacteria on putrescine and other polyamine formation by foodborne pathogens in ornithine decarboxylase broth.conversion of ornithine to putrescine by salmonella paratyphi a, listeria monocytogenes, staphylococcus aureus, and escherichia coli was investigated in ornithine decarboxylase broth (odb) using cell-free supernatants (cfss) obtained from leuconostoc mesenterodies subsp. cremoris, pediococcus acidilactici, lactococcus lactis subsp. lactis, streptococcus thermophilus. two groups of cell-free supernatants (25 or 50%) and control (only odb) were prepared to investigate putrescine (put) and other po ...201526043863
short communication: no antimicrobial effects from one source of commercial dried distillers grains with solubles.because residual antibiotics in dried distillers grains with solubles (ddgs) could lead to inadvertent feeding of antibiotics to animals, the objective of our study was to determine if a commercial ddgs contained antibiotics. the ddgs used in a feeding study, and milk from cows fed the ddgs, were below the detection limits for at least 17 antibiotics. additionally, we evaluated if ddgs had any antimicrobial effect against salmonella typhimurium, listeria innocua, escherichia coli atcc 25922, sta ...201526454305
[screening, combination of microbial deodorizer and the optimization of its deodorizing conditions].in order to obtain the candidate strains of deodorizing microorganism, three optimization strains with relatively higher deodorizing capacity were isolated from landfill leachate, which were given the labels of cc3, cc7, cc13 and cc16. according to the results of morphological observation, physiological and biochemical tests, 16s rdna sequence homology analysis, the strains cc3, cc7, cc13 and cc16 were identified as pediococcus acidilactici, bacillus megaterium, lactobacillus acidophilus and alc ...201525898673
transfer, composition and technological characterization of the lactic acid bacterial populations of the wooden vats used to produce traditional stretched cheeses.the biofilms of 12 wooden vats used for the production of the traditional stretched cheeses caciocavallo palermitano and pdo vastedda della valle del belìce were investigated. salmonella spp. and listeria monocytogenes were never detected. total coliforms were at low numbers with escherichia coli found only in three vats. coagulase-positive staphylococci (cps) were below the enumeration limit, whereas lactic acid bacteria (lab) dominated the surfaces of all vats. in general, the dominance was sh ...201526338114
bacterial diversity and mycotoxin reduction during maize fermentation (steeping) for ogi production.bacterial diversity and community structure of two maize varieties (white and yellow) during fermentation/steeping for ogi production, and the influence of spontaneous fermentation on mycotoxin reduction in the gruel were studied. a total of 142 bacterial isolates obtained at 24-96 h intervals were preliminarily identified by conventional microbiological methods while 60 selected isolates were clustered into 39 otus consisting of 15 species, 10 genera, and 3 phyla by 16s rrna sequence analysis. ...201526697001
assessing quality of medicago sativa silage by monitoring bacterial composition with single molecule, real-time sequencing technology and various physiological parameters.the present study applied the pacbio single molecule, real-time sequencing technology (smrt) in evaluating the quality of silage production. specifically, we produced four types of medicago sativa silages by using four different lactic acid bacteria-based additives (ad-i, ad-ii, ad-iii and ad-iv). we monitored the changes in ph, organic acids (including butyric acid, the ratio of acetic acid/lactic acid, γ-aminobutyric acid, 4-hyroxy benzoic acid and phenyl lactic acid), mycotoxins, and bacteria ...201627340760
pediocin sa-1: a selective bacteriocin for controlling listeria monocytogenes in maize silages.in this study, we assessed the potential as silage additive of a bacteriocin produced by pediococcus acidilactici northern regional research laboratory (nrrl) b-5627 (pediocin sa-1). maize was inoculated either with a bacterial starter alone (i) or in combination with the bacteriocin (ip), and untreated silage served as control. we monitored the products of fermentation (ethanol, and lactic and acetic acids), the microbial population, and the presence of the indicator strain listeria monocytogen ...201627522409
potential of lactic acid bacteria at regulating escherichia coli infection and inflammation of bovine endometrium.about 40% of dairy cattle develop uterine disease during postpartum period, causing infertility. some studies indicate that uterine infection, predominantly by escherichia coli in the first week postpartum, is associated with metritis, an uterus inflammation in which the cow fails to completely clear bacterial contaminants. the aim of this study was to evaluate the potential of four lactic acid bacteria (lab) (lactobacillus rhamnosus, pediococcus acidilactici, lactobacillus reuteri, and lactobac ...201626549120
evaluation of potential probiotic characteristics and antibacterial effects of strains of pediococcus species isolated from broiler chickens.pediococcus spp. were isolated from poultry rectum, faeces and food as good probiotic candidates in order to select strains to be used as probiotic in poultry feed. a total of 168 lactic acid bacteria were isolated and 51 isolates including 31 lactobacillus spp. and 20 pediococcus spp. were able to survive in low ph and bile salt concentration. the pediococcus spp. were identified and their ability to form biofilm, adhesion to caco-2 cells and antimicrobial activities against enteric pathogenic ...201627057800
isolation, identification and screening of potential probiotic bacteria in milk from south african saanen goats.this study aimed to evaluate lactic acid bacteria isolates from saanen goats' milk for probiotic attributes, thereby determining their potential as direct-fed microbials for goats. isolates were identified using api 50ch system, 16s rdna sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry. all 17 isolates obtained were identified as lactobacillus plantarum except one identified as pediococcus acidilactici. four isolates identified as l. plantarum (accessio ...201627981504
potential antimicrobial and antiproliferative activities of autochthonous starter cultures and protease epg222 in dry-fermented sausages.this work studied the presence of nitrogen compounds with bioactive properties in iberian pork sausages that were manufactured using different autochthonous starter cultures (pediococcus acidilactici ms200 and staphylococcus vitulus rs34) and protease epg222. nitrogen compounds were extracted and evaluated for their antimicrobial effect against spoilage and pathogenic bacteria, such as bacillus cereus, escherichia coli, salmonella choleraesuis, staphylococcus aureus and listeria monocytogenes, a ...201627112426
antifungal activity of lactic acid bacteria strains isolated from natural honey against pathogenic candida species.the role of lactic acid bacteria (lab) in honey as antifungal activity has received little attention and their mechanism of inhibitory of fungi is not fully understood. in this study, lab were isolated from honey samples from malaysia, libya, saudi arabia, and yemen. twenty-five isolates were confirmed lab by catalase test and gram staining, and were screened for antifungal activity. four lab showed inhibitory activity against candida spp. using the dual agar overlay method. and they were identi ...201628154488
using in vitro immunomodulatory properties of lactic acid bacteria for selection of probiotics against salmonella infection in broiler chicks.poultry is known to be a major reservoir of salmonella. the use of lactic acid bacteria has become one of successful strategies to control salmonella in poultry. the purpose of this study was to select lactic acid bacteria strains by their in vitro immunomodulatory properties for potential use as probiotics against salmonella infection in broiler chicks. among 101 isolated lactic acid bacteria strains, 13 strains effectively survived under acidic (ph 2.5) and bile salt (ranging from 0.1% to 1.0% ...201626799658
survival and metabolic activity of pediocin producer pediococcus acidilactici ul5: its impact on intestinal microbiota and listeria monocytogenes in a model of the human terminal ileum.pediococcus acidilactici ul5 is a promising probiotic candidate due to its high survival rate under gastric and duodenal conditions and to its ability to produce the antilisterial bacteriocin pediocin pa-1. its survival, metabolic activity, and impact on listeria monocytogenes in a continuous stirred tank reactor containing immobilized human intestinal microbiota were studied over a period of 32 days of feeding a nutrient medium simulating ileal chyme. the impact of p. acidilactici ul5 on differ ...201626162534
influence of environmental factors on bacteriocin production by human isolates of lactococcus lactis mm19 and pediococcus acidilactici mm33.the influence of temperature, initial ph, and carbon and nitrogen sources on bacteriocin secreted by lactococcus lactis mm19 (mm19) and pediococcus acidilactici mm33 (mm33) was evaluated. it was found that 30 and 45 °c were the growth temperatures for higher nisin and pediocin production by mm19 and mm33, respectively. the initial ph values for higher production of nisin and pediocin were 9 and 6, respectively. glucose and wheat peptone e430 were found as suitable carbon and nitrogen sources, re ...201626686688
lactic acid bacteria in dried vegetables and spices.spices and dried vegetable seasonings are potential sources of bacterial contamination for foods. however, little is known about lactic acid bacteria (lab) in spices and dried vegetables, even though certain lab may cause food spoilage. in this study, we enumerated lab in 104 spices and dried vegetables products aimed for the food manufacturing industry. the products were obtained from a spice wholesaler operating in finland, and were sampled during a one-year period. we picked isolates (n = 343 ...201626678137
a prebiotic matrix for encapsulation of probiotics: physicochemical and microbiological study.this work aims to develop an encapsulated oral-synbiotic supplement by studying the effect of adding inulin in alginate beads and observing its ability to protect three probiotic strains: pediocucus acidilactici, lactobacillus reuteri and lactobacillus salivarius. beads of different inulin concentrations 0%, 5%, 10%, 15% and 20% (w/v) in 2% (w/v) alginate solution were prepared by the extrusion/ionotropic gelation method. polymer distribution within beads was characterised using confocal laser s ...201626805512
aqueous two-phase flotation for primary recovery of bacteriocin-like inhibitory substance (blis) from pediococcus acidilactici kp10.an aqueous two-phase flotation (atpf) system based on polyethylene glycol (peg) and sodium citrate (nano3c6h5o7·2h2o) was considered for primary recovery of bacteriocin-like inhibitory substance (blis) from pediococcus acidilactici kp10. the effects of atpf parameters namely phase composition, tie-line length (tll), volume ratio between the two phases (vr), amount of crude load (cl), ph, nitrogen gas flow rate (fr) and flotation time (ft) on the performance of recovery were evaluated. blis was m ...201627262666
the effects of feeding with synbiotic (pediococcus acidilactici and fructooligosaccharide) enriched adult artemia on skin mucus immune responses, stress resistance, intestinal microbiota and performance of angelfish (pterophyllum scalare).the aim of this study was to evaluate the effects of feeding on synbiotic (pediococcus acidilactici and fructooligosaccharide) enriched adult artemia franciscana on skin mucus immune responses, stress resistance, intestinal microbiota and growth performance of angelfish (pterophyllum scalare). three hundred and sixty fish with initial weight 3.2 ± 0.13 g were randomly divided into twelve aquaria (50 l) assigned to four groups in triplicates. fish were fed for 7 weeks with dietary treatments, inc ...201627150050
dipeptidyl peptidase-ii from probiotic pediococcus acidilactici: purification and functional characterization.dipeptidylpeptidase-ii (dpp-ii, e.c. 3.4.14.2), an exopeptidase was purified 15.4 fold with specific activity and yield of 15.4u/mg/ml and 14.68% respectively by a simple two step procedure from a probiotic pediococcus acidilactici. dpp-ii is 38.7kda homodimeric serine peptidase with involvement of his and subunit mass of 18.9kda. the enzyme exhibited optimal activity at ph 7.0 and 37°c with activation energy of 24.97kj/mol. the enzyme retained more than 90% activity upto 50°c thus adding indust ...201627640091
characteristics of isolated lactic acid bacteria and their effects on the silage quality.four lactic acid bacteria (lab) strains isolated from common vetch, tall fescue and perennial ryegrass on the tibetan plateau were characterized, and their effects on the fermentation quality of italian ryegrass (lolium multiflorum lam.) silage were studied.201627764915
lactic acid bacteria in total mixed ration silage containing soybean curd residue: their isolation, identification and ability to inhibit aerobic deterioration.we investigated the effects of the predominant lactic acid bacteria (lab) on the fermentation characteristics and aerobic stability of total mixed ration (tmr) silage containing soybean curd residue (sc-tmr silage). the sc-tmr materials were ensiled in laboratory silos for 14 or 56 days. lab predominant in sc-tmr silage were identified (exp. 1). lactobacillus fermentum (l. fermentum) and streptococcus bovis (s. bovis) were found in the untreated materials, leuconostoc pseudomesenteroides (l. pse ...201626949952
evaluation of mono or mixed cultures of lactic acid bacteria in type ii sourdough system.the aim of this study was to evaluate the use of mono and mixed lactic acid bacteria (lab) cultures to determine suitable lab combinations for a type ii sourdough system. in this context, previously isolated sourdough lab strains with antimicrobial activity, which included lactobacillus plantarum pfc22, lactobacillus brevis pfc31, pediococcus acidilactici pfc38, and lactobacillus sanfranciscensis pfc80, were used as mono or mixed culture combinations in a fermentation system to produce type ii s ...201625807196
interactions between candidate probiotics and the immune and antioxidative responses of european sea bass (dicentrarchus labrax) larvae.the use of lactic acid bacteria (lab) as probiotics in aquaculture may improve the quality of seed production and limit the use of antibiotics in fish hatcheries. this study attempted to further characterize the candidate probiotic lactobacillus casei x2, and the immune and physiological responses of the sea bass larvae. l. casei x2 was confirmed as a good candidate, due to its wide antibacterial spectrum against both gram-positive and gram-negative bacteria, and its free radical scavenging acti ...201627133829
can the development and autolysis of lactic acid bacteria influence the cheese volatile fraction? the case of grana padano.in this study, the relationship between the dynamics of the growth and lysis of lactic acid bacteria in grana padano cheese and the formation of the volatile flavor compounds during cheese ripening was investigated. the microbial dynamics of grana padano cheeses that were produced in two different dairies were followed during ripening. the total and cultivable lactic microflora, community composition as determined by length heterogeneity-pcr (lh-pcr), and extent of bacterial lysis using an intra ...201627299670
probiotic effect on calves infected with salmonella dublin: haematological parameters and serum biochemical profile.the aim of this study was to evaluate the effect of a probiotic/lactose inoculum on haematological and immunological parameters and renal and hepatic biochemical profiles before and during a salmonella dublin dspv 595t challenge in young calves. twenty eight calves, divided into a control and probiotic group were used. the probiotic group was supplemented with 100 g lactose/calf/d and 10(10) cfu/calf/d of each strain of a probiotic inoculum composed of lactobacillus casei dspv318t, lactobacillus ...201626615854
selection of lactic acid bacteria isolated from tunisian cereals and exploitation of the use as starters for sourdough fermentation.wheat bread is the most popular staple food consumed in tunisia and, despite the niche production of some typical breads (e.g. tabouna, mlawi, mtabga), the major part is currently produced with baker's yeast at industrial or, mainly, at artisanal level, while the use of sourdough fermentation is rarely reported. considering the growing national demand for cereal baked goods, it can be hypothesized that sourdough fermentation through the use of selected lactic acid bacteria as starters could impr ...201626974248
pcr of crtnm combined with analytical biochemistry: an efficient way to identify carotenoid producing lactic acid bacteria.lactic acid bacteria (lab) synthesize a wide variety of biochemical compounds during food fermentation. carotenoids provide important biological functions for bacteria, and their consumption by humans has many beneficial effects. in this study, the presence of several genes involved in the production of carotenoids was determined by blast analysis and pcr in a collection of 156 lab isolated from traditional amylaceous african fermented foods. only the crte gene and the crtnm operon were present ...201626776108
influence of starter culture and a protease on the generation of ace-inhibitory and antioxidant bioactive nitrogen compounds in iberian dry-fermented sausage "salchichón".the effect of the addition of an autochthonous starter culture and the protease epg222 on the generation of angiotensin-i-converting enzyme (ace)-inhibitory and antioxidant compounds by the dry-fermented sausage "salchichón" was investigated. sausages were prepared with purified epg222 and pediococcus acidilactici ms200 and staphylococcus vitulus rs34 as the starter culture (p200s34), separately and together, ripened for 90 days, and compared to a control batch. among the ripening time points (2 ...201627441267
characterization of pediococcus acidilactici strains isolated from rainbow trout (oncorhynchus mykiss) feed and larvae: safety, dna fingerprinting, and bacteriocinogenicity.the use of lactic acid bacteria (lab) as probiotics constitutes an alternative or complementary strategy to chemotherapy and vaccination for disease control in aquaculture. the objectives of this work were (1) the in vitro safety assessment of 8 pediococcus acidilactici strains isolated from rainbow trout (oncorhynchus mykiss, walbaum) feed and larvae; (2) the evaluation of their genetic relatedness; (3) the study of their antimicrobial/bacteriocin activity against fish pathogens; and (4) the bi ...201627137071
effects of lactic acid bacteria with bacteriocinogenic potential on the fermentation profile and chemical composition of alfalfa silage in tropical conditions.the fermentation profile, chemical composition, and microbial populations of alfalfa silages treated with microbial inoculants (mi) at different fermentation periods (t) were evaluated in tropical conditions. a 4×6 factorial arrangement was used in a randomized design with 3 replicates. fresh alfalfa was treated with (1) no treatment (ctrl), (2) commercial inoculant (cin), (3) pediococcus acidilactici (strain 10.6, s1), and (4) pediococcus pentosaceus (strain 6.16, s2). an inoculant application ...201626774723
antimicrobial efficacy of an array of essential oils against lactic acid bacteria.the essential oils of clove bud, cinnamon bark and thyme, and their individual compounds including allyl isothiocyanate (ait), carvacrol, cinnamaldehyde, cinnamic acid, eugenol, and thymol were initially assessed for antimicrobial activity against 9 lactic acid bacteria (lab) species. carvacrol and thymol were the most inhibitory with mics of 0.1% (v/v and w/v, respectively). cinnamaldehyde, cinnamon bark oil, clove bud oil, eugenol, and thyme oil were moderately inhibitive (mics = 0.2% v/v), wh ...201626749216
engineering wild-type robust pediococcus acidilactici strain for high titer l- and d-lactic acid production from corn stover feedstock.pediococcus acidilactici ty112 producing l-lactic acid and p. acidilactici zp26 producing d-lactic acid, were engineered from the wild-type p. acidilactici dq2 by ldhd or ldh gene disruption, and the robustness of the wild-type strain to the inhibitors derived from lignocellulose pretreatment was maintained well. in simultaneous saccharification and fermentation (ssf), 77.66 g l(-1) of l-lactic acid and 76.76 g l(-1) of d-lactic acid were obtained at 25% (w/w) solids content of dry dilute acid p ...201626616423
agrowaste to vanillin conversion by a natural pediococcus acidilactici strain bd16.owing to its flavoring, antimicrobial, antioxidant and anticarcinogenic nature, vanillin is widely used in foods, beverages, perfumes and pharmaceutical products. ferulic acid (fa) is an important precursor of vanillin which is abundant in cereals like maize, rice and wheat and sugar beet. a major drawback of microbial vanillin production from fa is the degradation and biotransformation of toxic vanillin to other phenolic derivatives. the present study is undertaken to explore microbial vanillin ...201627734757
[therapeutic application of the preparation d 1 s in cardio-vascular neurosis]. 20165018516
root canal preparation. 20164881433
[presence of antibodies to fowl proteins in the workers on an industrial fattening farm]. 20164997791
proceedings of the ceremony honoring alex m. burgess, m.d. at the miriam hospital, providence, r. i. may 20, 1970. 20164920031
[respiratory system--disease model by medical engineering approach]. 20165064636
a probability model for the fusion of nucleoli in metabolic cells. 20164844621
on hearing, presenting and discussing scientific papers. 20165077127
[supraglottal horizontal laryngectomy in the treatment of supraglottal cancers of the laryngeal border. (apropos of 104 cases)]. 20165047636
[evolution of the anlagens of chick embryo limbs treated with nitrogen yperite at the 48 h stage of incubation]. 20164997328
intestinal segments and pouches in gastrointestinal surgery. 20164923133
[300th anniversary of lucka pharmacy]. 20164931013
hospital matrons--unlimited hours--appeal judgment of the industrial court. 20164973939
dorsal dislocation of the metacarpophalangeal joint of the index finger. 20164793658
an application of general systems concepts to psychoanalysis. 20164723249
the "germ of laziness" in the south, 1900-1915: charles wardell stiles and the progressive paradox. 20164932199
fingertip hyperesthesia due to scouring pad. 20165107635
observations on complications after hallux valgus surgery. 20164826079
[reflections on transplantation of the lung]. 20164946076
[importance of parasitic diseases]. 20164782086
idioventricular tachycardia with some unusual features. 20164743960
oral administration of the lactic acid bacterium pediococcus acidilactici attenuates atherosclerosis in mice by inducing tolerogenic dendritic cells.the intestinal microbiota appears to play an important role in the development of atherosclerosis. we investigated the effect of the probiotic lactic acid bacterium pediococcus acidilactici r037 on atherosclerosis using apolipoprotein e-deficient (apoe (-/-)) mice. six-week-old apoe (-/-) mice were orally administered r037 six times a week. mice treated with r037 for 12 weeks exhibited markedly attenuated atherosclerotic lesions in the aortic root (2.3 ± 0.15 × 10(5) µm(2) vs. 3.3 ± 0.29 × 10(5) ...201728181012
a combination of lactic acid bacteria regulates escherichia coli infection and inflammation of the bovine endometrium.uterine function in cattle is compromised by bacterial contamination and inflammation after calving. the objective of this study was to select a combination of lactic acid bacteria (lab) to decrease endometrium inflammation and escherichia coli infection. primary endometrial epithelial cells were cultured in vitro to select the most favorable lab combination modulating basal tissue inflammation and e. coli infection. supernatants were obtained to determine expression of pro-inflammatory cytokine ...201727837977
whole genome sequence of lactic acid bacterium pediococcus acidilactici strain s1.pediococcus acidilactici strain s1, a lactic acid-fermenting bacterium, was isolated from makgeolli-a korean traditional fermented alcoholic beverage. here we report the 1,980,172bp (g+c content, 42%) genome sequence of pediococcus acidilactici strain s1 with 1,525 protein-coding sequences (cds), of which 47% could be assigned to recognized functional genes. the genome sequence of the strain s1 might provide insights into the genetic basis of the lactic acid bacterium with alcohol-tolerant.201728256390
comparative effect of the i3.1 probiotic formula in two animal models of colitis.use of probiotic therapy is an active area of investigation to treat intestinal disorders. the clinical benefits of the i3.1 probiotic formula (lactobacillus plantarum (cect7484, cect7485) and p. acidilactici (cect7483)) were demonstrated in irritable bowel syndrome (ibs) patients in a randomized, double-blind, placebo-controlled clinical trial. the aim of this study was to evaluate the therapeutic effects of i3.1 in two experimental models of colitis, a dextran sulfate sodium (dss)-induced coli ...201727832441
draft genome sequence of alcohol-tolerant bacteria pediococcus acidilactici strain k3.pediococcus acidilactici strain k3 is an alcohol-tolerant lactic acid bacterium isolated from nuruk, which is a traditional korean fermentation starter for makgeolli brewing. draft genome of this strain was approximately 1,991,399bp (g+c content, 42.1%) with 1525 protein-coding sequences (cds), of which 44% were assigned to recognized functional genes. this draft genome sequence data of the strain k3 will provide insights into the genetic basis of its alcohol-tolerance.201728043774
growth performance, nutrients digestibility, and blood metabolites of lambs fed diets supplemented with probiotics during pre- and post-weaning period.two experiments were conducted to evaluate the effects on growth performance, digestibility, and blood metabolites of lambs during pre- and post-weaning period of inclusion of a commercial probiotic (pro) containing a mixture of two strains of pediococcus, pediococcus acidilactici (1×10(6) colony-forming unit [cfu]/g) and pediococcus pentosaceus (1.3×10(6) cfu/g), with dextrose as the carrier compound compared to a diet based on concentrate mixture and wheat straw.201728002935
characteristics of lactic acid bacteria isolates and their effect on the fermentation quality of napier grass silage at three high temperatures.the poor fermentation quality of silage is an important issue for silage production during the high temperatures of summer. pediococcus acidilactici gg13 (gg13) and lactobacillus rhamnosus gg26 (gg26) isolated from italian ryegrass (lolium multiflorum lam.) silage were characterised by morphological and physiological tests and 16s rrna sequencing analysis, and their effects, along with those of a commercial lactic acid bacteria (lab) inoculant (cb), on the fermentation quality of facultative hal ...201727539868
pediococcus acidilactici lab4 and lactobacillus plantarum lab12 assimilate cholesterol and modulate abca1, cd36, npc1l1 and scarb1 in vitro.there is growing interest in the use of probiotic lactic acid bacteria (lab) for prevention of hypercholesterolaemia. this study assessed the cholesterol lowering ability of pediococcus acidilactici lab4 and lactobacillus plantarum lab12 in growth media. both lab yielded >98% (39.2 μg/ml) cholesterol lowering in growth media. nile red staining indicated direct assimilation of cholesterol by the lab. the lab were then explored for their prophylactic (pre-treatment of ht29 cells with lab prior to ...201727903090
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