Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
effectiveness of a salt transport cascade in the renal medulla: computer simulations.based on morphological observations, it has been concluded that the upper parts of the long descending limbs of henle's loops should be able to secrete salts into the tubular fluid (kriz, w. federation proc. 42:2379-2385, 1983). in the same article, a hypothesis is given depicting how this active transport in concert with certain characteristics of the medullary architecture might produce a transport cascade toward the papillary tip, thus supporting the accumulation of salts in this region. the ...20133591954
hospitals say labor proposal will cost money. 20133679120
acute steroid-induced tetraplegia following status asthmaticus.a 10-year-old girl was treated for an acute asthmatic attack with ventilation and a high-dose steroids regimen. an areflexic paralysis of the four limbs was noted when artificial ventilatory support was withdrawn. electromyography and a muscle biopsy revealed marked myopathic features. we discuss the unusual presentation of an acute steroid-induced myopathy in an asthmatic patient.20133725482
[benign familial neonatal convulsions, febrile crises and epilepsy. their coincidence in a family]. 20134062078
[giessen test of the aging dynamics of social behavior].the giessen-test was applied during gerontological complex investigations to study the biological ages of predominantly professionally engaged leipzig citizens. four dimensions of this psycho-diagnostical procedure showed a clear age-characteristic confirming the tendencially known features of psycho-social ageing. the achieved results point again to the fact that the deficit-model of ageing processes is not justified in psycho-social relations, in particular. the experiences of this experimenta ...20134049927
[giessen test of the aging dynamics of social behavior].the giessen-test was applied during gerontological complex investigations to study the biological ages of predominantly professionally engaged leipzig citizens. four dimensions of this psycho-diagnostical procedure showed a clear age-characteristic confirming the tendencially known features of psycho-social ageing. the achieved results point again to the fact that the deficit-model of ageing processes is not justified in psycho-social relations, in particular. the experiences of this experimenta ...20134049927
calculation of substrate binding affinities for a bacterial gh78 rhamnosidase through molecular dynamics simulations.ram2 from pediococcus acidilactici is a rhamnosidase from the glycoside hydrolase family 78. it shows remarkable selectivity for rutinose rather than para-nitrophenyl-alpha-l-rhamnopyranoside (p-npr). molecular dynamics simulations were performed using a homology model of this enzyme, in complex with both substrates. free energy calculations lead to predicted binding affinities of -34.4 and -30.6 kj mol(-1) respectively, agreeing well with an experimentally estimated relative free energy of 5.4  ...201323914137
a novel thermotolerant pediococcus acidilactici b-25 strain for color, cod, and bod reduction of distillery effluent for end use applications.the present study was aimed to characterize physico-chemical and microbial population of distillery effluent and isolate a novel thermotolerant bacterium for color, cod, and bod reduction of spentwash. the level of alkalinity, tss, do, cod, bod, tn, ammonical nitrogen, nitrate nitrogen, phosphorous, potassium, chloride, and calcium of spentwash (sw), bioreactor effluent (be), and secondary treated effluent (ste) were well above the permissible limits. the level of color, ts, and tds were under t ...201323224419
blpc-regulated bacteriocin production in streptococcus thermophilus.streptococcus thermophilus b59671 produces a bacteriocin with anti-pediococcal activity, but genes required for its production are not characterized. genome sequencing of s. thermophilus has identified a genetic locus encoding a quorum sensing (qs) system that regulates production of class ii bacteriocins. however, in strains possessing this gene cluster, production of bacteriocin like peptides (blp) was only observed when excess pheromone was provided. pcr analysis revealed this strain possesse ...201323183916
simultaneous saccharification and high titer lactic acid fermentation of corn stover using a newly isolated lactic acid bacterium pediococcus acidilactici dq2.a lactic acid bacterium with high tolerance of temperature and lignocellulose derived inhibitor was isolated and characterized as pediococcus acidilactici dq2. the strain used in the simultaneous saccharification and fermentation (ssf) for high titer lactic acid production at the high solids loading of corn stover. corn stover was pretreated using the dry sulphuric acid pretreatment, followed by a biological detoxification to remove the inhibitors produced in the pretreatment. the bioreactor wit ...201323127836
statistical media and process optimization for biotransformation of rice bran to vanillin using pediococcus acidilactici.an isolate of p. acidilactici capable of producing vanillin from rice bran was isolated from a milk product. response surface methodology was employed for statistical media and process optimization for production of biovanillin. statistical medium optimization was done in two steps involving placket burman design and central composite response designs. the rsm optimized vanillin production medium consisted of 15% (w/v) rice bran, 0.5% (w/v) peptone, 0.1% (w/v) ammonium nitrate, 0.005% (w/v) feru ...201324416928
bio-preservative and therapeutic potential of pediocin: recent trends and future perspectives.bacteriocins produced by lactic acid bacteria are of keen interest to the food industry for their bio-preservative potential and antimicrobial properties. the increasing demand for high quality 'safe' foods which are not extensively processed has created a niche for natural food preservatives. the bacteriocins (produced by bacteria) derived their name after the genera that produce them, hence bacteriocins produced by genus pediococcus are known as pediocin. pediocins are antimicrobial peptides w ...201324182324
in vitro and in vivo survival and colonic adhesion of pediococcus acidilactici mtcc5101 in human gut.the present study aims to investigate the probiotic nature of pediococcus acidilactici mtcc5101 by an in vitro assay of bacterial adherence to intestinal epithelial cells of human gastrointestinal (gi) tract using caco-2 cell line. further to assess the in vivo survival in the gi tract, oral feeding was carried out with the help of 10 healthy volunteers. the effect on wellness was assessed by studying blood biochemical parameters of the volunteers. the survival of the bacteria was assessed using ...201324175293
dietary synbiotic application modulates atlantic salmon (salmo salar) intestinal microbial communities and intestinal immunity.a feeding trial was conducted to determine the effect of dietary administration of pediococcus acidilactici ma18/5m and short chain fructooligosaccharides (scfos) on atlantic salmon (salmo salar l.) intestinal health. salmon (initial average weight 250 g) were allocated into triplicate sea pens and were fed either a control diet (commercial diet: 45% protein, 20% lipid) or a synbiotic treatment diet (control diet + p. acidilactici at 3.5 g kg(-1) and 7 g kg(-1) scfos) for 63 days. at the end of ...201324161776
the effect of pediococcus acidilactici bacteria used as probiotic supplement on the growth and non-specific immune responses of green terror, aequidens rivulatus.a 56-day feeding trial was conducted on a species of ornamental fish called green terror (aequidens rivulatus) (0.388 ± 0.0021 g) to assess the effect of probiotic bacteria, pediococcus acidilactici on the growth indices and innate immune response. the fish were randomly allocated into 9 oval tanks (120 l) at a density of 60 fish per tank. the experimental diets were comprised of the control (c), c complemented with fish oil (o) and the probiotic and fish oil (pa) and fed ad lib twice a day. the ...201324161762
characterization of inulin hydrolyzing enzyme(s) in commercial glucoamylases and its application in lactic acid production from jerusalem artichoke tubers (jat).a high inulinase activity was found in three commercially available glucoamylase enzymes. its origin was investigated and two proteins in the commercial glucoamylases were identified as the potential enzymes showing inulinase activity. one of the commercial glucoamylases, ga-l new from genencor, was used for jerusalem artichoke tubers (jat) hydrolysis and a high hydrolysis yield of fructose was obtained. the simultaneous saccharification and lactic acid fermentation (ssf) of jat was carried out ...201324050923
probiotic pediococcus acidilactici modulates both localised intestinal- and peripheral-immunity in tilapia (oreochromis niloticus).the application of probiotics in aquaculture has received concerted research efforts but the localised intestinal immunological response of fish to probiotic bacteria is poorly understood. therefore, a study was conducted to evaluate the probiotic effect of pediococcus acidilactici on nile tilapia (oreochromis niloticus) with specific emphasis on intestinal health and probiotic levels as well as system level responses such as growth performance, feed utilization and haemato-immunological paramet ...201323871840
dietary probiotic supplementation modulated gut microbiota and improved growth of juvenile rainbow trout (oncorhynchus mykiss).in aquaculture, infectious diseases are the major cause of economic losses. probiotic supplementation may change the microbiota of the digestive tract and modulate the immune defences and nutritional performance. this study was conducted to evaluate the dietary supplementation of multi-species (a: bacillus sp., pediococcus sp., enterococcus sp., lactobacillus sp.) and single-species probiotics (b: pediococcus acidilactici) on growth performance and gut microbiota of rainbow trout (oncorhynchus m ...201323811044
draft genome sequence of the pediocin-encoding biopreservative and biocontrol strain pediococcus acidilactici d3.we describe a draft genome sequence for pediococcus acidilactici strain d3, a component of multistrain commercial cultures with biopreservative and biocontrol properties in food-based applications. strain d3 encodes at least one antimicrobial peptide, pediocin ampd3. the ampd3-encoding operon exhibits high sequence similarity to the archetype pediocin, pa-1, encoded by p. acidilactici pac 1.0.201323788534
exopolysaccharide produced by pediococcus acidilactici m76 isolated from the korean traditional rice wine, makgeolli.this work is aimed to increase knowledge of the functional exopolysaccharide (eps) from lactic acid bacteria (lab) in makgeolli, a korean fermented rice wine. among lab strains isolated from makgeolli, strain m76 was selected as a functional strain producing a bioactive eps, based on its antioxidative activity on the dpph radical. the 16s rrna gene sequencing analysis showed a high sequence similarity (99.0%) with p. acidilactici, but had different biochemical properties with the already known p ...201323648859
biotransformation of rice bran to ferulic acid by pediococcal isolates.ferulic acid (fa) is widely used in foods, in beverages, and in various pharmaceutical industries as a precursor of vanillin. fa biotransformation can occur during the growth of lactic acid bacteria (lab), and its conversion to other phenolic derivatives is observed by many scientists, where ferulic acid esterase (fae) and ferulic acid decarboxylase (fdc) play significant roles. the present study aimed at screening a panel of lab for their ability to release fa from rice bran, an agro waste mate ...201323615732
production and partial characterisation of an inducer-dependent novel antifungal compound(s) by pediococcus acidilactici lab 5.pediococcus acidilactici lab 5 produces an antifungal compound under in vitro conditions in an inducer-dependent manner. the main objective of the present study was to partially characterise this antifungal compound by uv-visible, ir, (1)h nmr, (13)c nmr and gc/ms analyses and also to assess its potentiality against a number of food spoilage, plant-pathogenic and human-pathogenic fungal species.201323423982
draft genome sequence of pediococcus lolii ngri 0510q(t) isolated from ryegrass silage.pediococcus lolii ngri 0510q(t) was isolated from ryegrass silage produced on ishigaki island, okinawa prefecture, japan. here we present a draft genome sequence for this strain, consisting of 103 contigs for a total of 2,047,078 bp, 2,154 predicted coding sequences, and a g+c content of 42.1%.201323405350
commercial biopreservatives combined with salt and sugar to control listeria monocytogenes during smoked salmon processing.three commercial antimicrobials, applied during the salting stage in the preparation of cold-smoked salmon, were investigated for their effect on the behavior of listeria monocytogenes. fresh salmon inoculated with l. monocytogenes inia 2530 was treated with three bacteriocin-based commercial biopreservatives, which were applied in combination with a salt-sugar mix. the product was kept at 8°c for 7 days. l. monocytogenes grew by approximately 3 log cfu/g in control salmon (without the salt-suga ...201323905807
antimicrobial properties of three lactic acid bacterial cultures and their cell free supernatants against listeria monocytogenes.control of listeria monocytogenes in ready-to-eat (rte) food products is a significant challenge and improved means for control are needed. in this study, the anti-listerial effects of three lactic acid bacteria (lab) were investigated. spot-on-lawn assays demonstrated the largest zones of inhibition against l. monocytogenes were produced by the pediococcus acidilactici strain, with zone diameters ranging from 13 to 18 mm. minimum inhibitory concentration (mic) experiments using cell free supern ...201323030442
growth, acid production and bacteriocin production by probiotic candidates under simulated colonic conditions.the aim of this study is to evaluate the capacity of three bacteriocin producers, namely lactococcus lactis subsp. lactis biovar diacetylactis ul719 (nisin z producer), l. lactis atcc 11454 (nisin a producer) and pediococcus acidilactici ul5 (pediocin pa-1 producer), and to grow and produce their active bacteriocins in macfarlane broth, which mimics the nutrient composition encountered in the human large intestine.201323167818
[production of l-lactic acid from pentose by a genetically engineered escherichia coli].in this study, we constructed a recombinant escherichia coli strain for the production of high-purity l-lactic acid, using a homoethanol fermenting mutant e. coli sz470 (deltafrdbc deltaldha deltaacka deltafoca-pflb deltapdhr: :pflbp6-pflbrbs-aceef-lpd) as the starting strain.201323858707
homofermentative production of optically pure l-lactic acid from xylose by genetically engineered escherichia coli b.polylactic acid (pla), a biodegradable polymer, has the potential to replace (at least partially) traditional petroleum-based plastics, minimizing "white pollution". however, cost-effective production of optically pure l-lactic acid is needed to achieve the full potential of pla. currently, starch-based glucose is used for l-lactic acid fermentation by lactic acid bacteria. due to its competition with food resources, an alternative non-food substrate such as cellulosic biomass is needed for l-la ...201323758664
influence of diets to wistar rats supplemented with soya, flaxseed and lupine products treated by lactofermentation to improve their gut health.the present study proposes the contribution of lactic acid bacteria and plants rich in bioactive substances and high-quality proteins as alternative products for human diets in improving the gut environment as potential against pathogenic bacteria. the effect of diets supplemented with soya, flaxseed and lupine flours fermented with a pediococcus acidilactici ktu05-7 probiotic strain in the gastrointestinal tract (git) of wistar rats were analyzed. in vivo experiments showed a positive effect of ...201323480304
catabolism of serine by pediococcus acidilactici and pediococcus pentosaceus.the ability to produce diacetyl from pyruvate and l-serine was studied in various strains of pediococcus pentosaceus and pediococcus acidilactici isolated from cheese. after being incubated on both substrates, only p. pentosaceus produced significant amounts of diacetyl. this property correlated with measurable serine dehydratase activity in cell extracts. a gene encoding the serine dehydratase (dsda) was identified in p. pentosaceus, and strains that showed no serine dehydratase activity carrie ...201323241976
effects of lactobacillus salivarius, lactobacillus reuteri, and pediococcus acidilactici on the nematode caenorhabditis elegans include possible antitumor activity.this study examined the effects of three lactic acid bacteria (lab) strains on the nematode caenorhabditis elegans. lactobacillus salivarius, lactobacillus reuteri, and pediococcus acidilactici were found to inhibit the development and growth of the worm. compared to escherichia coli used as the control, l. reuteri and p. acidilactici reduced the lifespan of wild-type and short-lived daf-16 worms. on the contrary, l. salivarius extended the lifespan of daf-16 worms when used live, but reduced it ...201322923095
highly specific culture-independent detection of ygngv motif-containing pediocin-producing strains.a novel method based on (1) initial microbiological screening and (2) a highly specific pcr is described for selection of strains expressing ygngv motif-containing pediocin. initial screening is carried out using spot on the lawn assay for selection of acid-free, hydrogen peroxide (h2o2)-free and secreted heat-stable inhibitory activity producing strains. this is followed by highly specific pcr for amplification of 406-bp fragment using forward primer: 5'-tggccaatatcattggtggt-3' targeting signal ...201326782603
comparison of lipase production by enterococcus faecium mtcc 5695 and pediococcus acidilactici mtcc 11361 using fish waste as substrate: optimization of culture conditions by response surface methodology.a medium using fish waste as substrate was designed for production of lipase by enterococcus faecium mtcc 5695 and pediococcus acidilactici mtcc 11361. medium components and culture conditions (fish waste protein hydrolysate (fwph) concentration, fish waste oil (fwo) concentration, ph, temperature, and fermentation time) which affect lipase production were screened using factorial (5 factors ∗ 2 levels) design of which fwph concentration, fwo concentration, and fermentation time showed significa ...201325969788
detection of mobile genetic elements in pediocin pa-1 like producing lactic acid bacteria.to evaluate the presence of mobile genetic elements (mges) in intergeneric and interspecific pediocin producing lactic acid bacteria (lab) the flanking regions of the pediocin pa-1/ach (pediocin pa-1) operon was characterized. in enterococcus faecium acr4 and lactobacillus plantarum acr2 a variation in the amplicon size in the downstream region of the operon was identified, suggesting a deletion in this region. beyond that, in pediocin pa-1 encoding plasmids mges such as islpl1 and mobilization ...201322915312
prebiotic content of bread prepared with flour from immature wheat grain and selected dextran-producing lactic acid bacteria.in the last few years the need to produce food with added value has fueled the search for new ingredients and health-promoting compounds. in particular, to improve the quality of bakery products with distinct nutritional properties, the identification of new raw materials, appropriate technologies, and specific microbial strains is necessary. in this study, different doughs were prepared, with 10% and 20% flour from immature wheat grain blended with type "0 america" wheat flour. immature flour w ...201323584774
diversity and dynamic of lactic acid bacteria strains during aging of a long ripened hard cheese produced from raw milk and undefined natural starter.the aim of this study was to explore diversity and dynamic of indigenous lab strains associated with a long ripened hard cheese produced from raw milk and undefined natural starter such as pdo grana padano cheese. samples of milk, curd, natural whey culture and cheeses (2nd, 6th, 9th and 13th months of ripening) were collected from 6 cheese factories in northern italy. dna was extracted from each sample and from 194 lab isolates. trna(ala)-23s rdna-rflp was applied to identify isolates. strain d ...201324010599
a description of the lactic acid bacteria microbiota associated with the production of traditional fermented vegetables in vietnam.an important part of the daily nourishment in vietnam constitutes of fermented vegetables. bacteria and especially lactic acid bacteria play a central role in the production of many fermented vegetables. the current study was conducted to investigate the diversity of native lactic acid bacteria (lab) populations in 'dua muoi' (mustard and beet fermentation) and 'ca muoi' (eggplant fermentation), three types of popular traditional fermented vegetables of vietnamese origin. to this end a polyphasi ...201323500611
fast identification of wine related lactic acid bacteria by multiplex pcr.the microflora of must and wine consists of yeasts, acetic acid bacteria and lactic acid bacteria (lab). the latter group plays an important role for wine quality. the malolactic fermentation carried out by lab leads to deacidification and stabilisation of wines. nevertheless, lab are often associated with wine spoilage. they are mainly responsible for the formation of biogenic amines. furthermore, some strains produce exopolysaccharide slimes, acetic acid, diacetyl and other off-flavours. in th ...201323122500
effects of bilberry (vaccinium myrtillus) in combination with lactic acid bacteria on intestinal oxidative stress induced by ischemia-reperfusion in mouse.intestinal ischemia-reperfusion (i/r) results in oxidative stress, inflammation, and tissue injuries. the present study investigates the antioxidative and anti-inflammatory effects of a dietary supplement of bilberry, either alone or in combination with lactobacillus plantarum reso56, l. plantarum heal19, or pediococcus acidilactici jam046, in an i/r-induced model for oxidative stress in mice. a bilberry diet without addition of bacteria significantly decreased both lipid peroxidation (p = 0.001 ...201323488931
characteristics of lactic acid bacteria isolates and their inoculating effects on the silage fermentation at high temperature.thermotolerant strains of lactic acid bacteria (lab) were screened for their suitability for silage making at high temperatures. four selected strains were identified by 16s rrna sequencing analyses and added to unwilted and wilted italian ryegrass for ensiling at 45°c. all four strains (ht1, ht2, ht3 and ht4) grew at 50°c and ph 4·0. strains ht1 and ht2 were identified as lactobacillus rhamnosus; ht3 and ht4 were identified as lact. fermentum. strains ht1 and ht2 were the most effective at impr ...201323106758
identification of a novel enzymatic activity from lactic acid bacteria able to degrade biogenic amines in wine.the main objectives of this study were the search for enzymatic activities responsible for biogenic amine (ba) degradation in lactic acid bacteria (lab) strains isolated from wine, their identification, and the evaluation of their applicability for reducing bas in wine. fifty-three percent of the 76 lab cell extracts showed activity against a mixture of histamine, tyramine, and putrescine when analyzed in-gel. the quantification of the degrading ability for each individual amine was tested in a ...201423515835
the microfloras and sensory profiles of selected protected designation of origin italian cheeses.approximately 39 italian cheeses carry protected designation of origin (pdo) status. these cheeses differ in their manufacturing technology and the microbial flora which comprise the finished products. the evolution of lactic microflora in cheeses with pdo status is of particular interest because the biochemical activities of these organisms participate in cheesemaking and may play an acknowledged role in the development of organoleptic characteristics during ripening. nonstarter lactic acid bac ...201426082116
the use of maldi-tof mass spectrometry, ribotyping and phenotypic tests to identify lactic acid bacteria from fermented cereal foods in abidjan (côte d'ivoire).matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) protein analysis, automated ribotyping, and phenotypic tests (e.g., cell morphology, gas production from glucose, growth and acid production on homofermemtative-heterofermentative differential (hhd) agar medium, sugar fermentation patterns) were used to identify 23 lactic acid bacteria (lab) isolated from fermented cereal foods available in abidjan, côte d'ivoire. pediococcus acidilactici (56.5%), lactoba ...201425279017
in situ production of pediocin pa-1 like bacteriocin by different genera of lactic acid bacteria in soymilk fermentation and evaluation of sensory properties of the fermented soy curd.the lactic acid bacteria (lab) are found to produce bacteriocins with enhanced nutritive properties in the fermented foods. in the present study, the ability of lab cultures (pediococcus acidilactici ncim 5424, enterococcus faecium ncim 5423 and lactobacillus plantarum acr2) to produce pediocin pa-1 like bacteriocin was evaluated during soymilk fermentation. the isolates e. faecium ncim 5423 and lb. plantarum acr2 were able to produce bacteriocin as well as ferment soymilk within 6 h of incubati ...201426396327
distillery spentwash decolorization by a noval consortium of pediococcus acidilactici and candida tropicalis under static condition.the aim of this study was to isolate a consortium of bacterium and yeast from natural resources for better decolorization of distillery spentwash. consortium exhibited 82±1.5% decolorization within 24 h when incubated at 45°c under static condition in effluent supplemented with 0.1%, glucose; 0.1%, peptone; 0.05%, mgso4; 0.01%. the cultures were identified as pediococcus acidilactici by 16s rdna analysis and candida tropicalis on the basis of phenotypic level. it is the first time when thermotol ...201426035951
effect of the oral intake of probiotic pediococcus acidilactici ba28 on helicobacter pylori causing peptic ulcer in c57bl/6 mice models.probiotic lactic acid bacteria are being proposed to cure peptic ulcers by reducing colonization of helicobacter pylori within the stomach mucosa and by eradicating already established infection. in lieu of that, in vitro inhibitory activity of pediocin-producing probiotic pediococcus acidilactici ba28 was evaluated against h. pylori by growth inhibition assays. further, chronic gastritis was first induced in two groups of c57bl/6 mice by orogastric inoculation with h. pylori with polyethylene c ...201424122711
co-culture fermentation of peanut-soy milk for the development of a novel functional beverage.most of the commercial probiotic products are dairy-based, and the development of non-dairy probiotic products could be an alternative for new functional products. the peanut-soy milk (psm(1)) was inoculated with six different lactic acid bacteria (lab), including probiotic strains and yeasts and fermentation was accomplished for 24h at 37 °c and afterwards, another 24h at ±4 °c. the pediococcus acidilactici (ufla bffcx 27.1), lactococcus lactis (cct 0360), lactobacillus rhamnosus (lr 32) probio ...201424984220
thermal tolerance of o157 and non-o157 shiga toxigenic strains of escherichia coli, salmonella, and potential pathogen surrogates, in frankfurter batter and ground beef of varying fat levels.the non-o157 shiga toxigenic escherichia coli (stec) serogroups most commonly associated with illness are o26, o45, o103, o111, o121, and o145. we compared the thermal tolerance (d55°c) of three or more strains of each of these six non-o157 stec serogroups with five strains of o157:h7 stec in 7% fat ground beef. d55°c was also determined for at least one heat-tolerant stec strain per serogroup in 15 and 27% fat ground beef. d55°c of single-pathogen cocktails of o157 and non-o157 stec, salmonella ...201425198841
metabolic engineering of pediococcus acidilactici bd16 for production of vanillin through ferulic acid catabolic pathway and process optimization using response surface methodology.occurrence of feruloyl-coa synthetase (fcs) and enoyl-coa hydratase (ech) genes responsible for the bioconversion of ferulic acid to vanillin have been reported and characterized from amycolatopsis sp., streptomyces sp., and pseudomonas sp. attempts have been made to express these genes in escherichia coli dh5α, e. coli jm109, and pseudomonas fluorescens. however, none of the lactic acid bacteria strain having gras status was previously proposed for heterologous expression of fcs and ech genes f ...201425077778
inhibitory activity of postbiotic produced by strains of lactobacillus plantarum using reconstituted media supplemented with inulin.the present study aimed to determine the inhibitory activity of postbiotic produced by l. plantarum using reconstituted media supplemented with different levels of inulin and to select the best combination based on the modified inhibitory activity (mau/ml) against pathogens.201424991236
pediococcus acidilactici ul5 and lactococcus lactis atcc 11454 are able to survive and express their bacteriocin genes under simulated gastrointestinal conditions.the aim of this work is to study the expression of stress genes and those involved in pediocin and nisin production in pediococcus acidilactici ul5 and lactococcus lactis atcc11454 under simulated gastrointestinal (gi) physiological conditions.201424279824
intravaginal lactic acid bacteria modulated local and systemic immune responses and lowered the incidence of uterine infections in periparturient dairy cows.the objective of this investigation was to evaluate whether intravaginal infusion of a lactic acid bacteria (lab) cocktail around parturition could influence the immune response, incidence rate of uterine infections, and the overall health status of periparturient dairy cows. one hundred pregnant holstein dairy cows were assigned to 1 of the 3 experimental groups as follows: 1) one dose of lab on wk -2 and -1, and one dose of carrier (sterile skim milk) on wk +1 relative to the expected day of p ...201425919010
purification and mass spectrometry based characterization of a pediocin produced by pediococcus acidilactici 13.bacteriocins are antimicrobial peptides produced by several bacterial species. among the bacteriocins pediocin-like bacteriocins have a significant inhibitory activity on the foodborne pathogens especially on listeria monocytogenes. this study aims to select a simple and usable purification method to purify/concentrate the antimicrobial peptide and characterization of the bacteriocin produced by pediococcus acidilactici 13 by using proteomic approaches which is a recent omic technology. for puri ...201425015266
the arginine deiminase pathway of koji bacteria is involved in ethyl carbamate precursor production in soy sauce.ethyl carbamate (ec) is a group 2a carcinogen generated from a few precursors in many fermented foods and alcoholic beverages. citrulline, urea, carbamoyl phosphate, and ethanol are common precursors detected in fermented foods. in this study, citrulline was proved to be the main ec precursor in soy sauce, which was found to be accumulated in moromi mash period and correlated with the utilization of arginine by koji bacteria. six koji isolates belonging to three genera were identified to be able ...201425048638
lipid-lowering effects of pediococcus acidilactici m76 isolated from korean traditional makgeolli in high fat diet-induced obese mice.the effect of pediococcus acidilactici m76 (lactic acid bacteria) isolated from makgeolli on mice fed a high fat diet was investigated to clarify the lipid lowering function. c57bl/6j male mice were randomly divided into a normal diet (nd) group, high fat diet (hd) group, hd plus pediococcus acidilactici dsm 20284 reference strain (pr) group, and hd plus pediococcus acidilactici m76 strain (pa) groups. the lyophilized pa and pr strain were dissolved in distilled water at a final concentration of ...201424609135
primary recovery of a bacteriocin-like inhibitory substance derived from pediococcus acidilactici kp10 by an aqueous two-phase system.a polymer-salt aqueous two-phase system (atps) consisting of polyethylene-glycol (peg) with sodium citrate was developed for direct recovery of a bacteriocin-like inhibitory substance (blis) from a culture of pediococcus acidilactici kp10. the influences of phase composition, tie-line length (tll), volume ratio (vr), crude sample loading, ph and sodium chloride (nacl) on the partition behaviour of blis was investigated. under optimum conditions of atps, the purification of blis was achieved at 2 ...201424423507
the use of affinity chromatography for the specific purification of antibodies and antigens. 20144184826
brotherly lumps. familial occurrence of chemodectoma. 20144295142
renal capsular leiomyoma causing painless microscopic hematuria. an unusual manifestation of a rare tumor. 20144033612
prognostic significance of the dna content in renal cell carcinoma.the dna content in renal cell carcinoma as an indicator of prognosis was studied retrospectively in 55 patients without distant metastases at operation. two groups of patients were selected, differing with respect to survival. thirty-three patients survived for at least 10 years and 22 succumbed to the disease within four years. dna measurements in morphologically identified tumor cells were performed in histological sections by single cell cytophotometry. the tumor cells of the surviving patien ...20143944883
expectations of the associate-degree graduate: a director of nursing's point of view. 20144151153
[diet therapy of celiac disease in children]. 20144034326
the epidemiology of gingivitis in children. 20144224057
the effects of own-home and institution-rearing on the behavioural development of normal and mongol children. 20144110578
cellular changes in the psoriatic epidermis. ix. neutron activation analysis of mercury in patients topically treated with ammonium mercuric chloride. 20144099141
[french language association for the study of diabetes and metabolic diseases. paris, 18-19 october 1985. abstracts]. 20144076511
editorial: prosopagnosia. 20144136673
[the significance of caries of six-year-molars in schoolchildren in zagreb]. 20144254300
cerebrovascular disease: changes in somatosensory evoked potentials associated with unilateral lesions. 20144126452
computer automated evaluation and prediction of the iball index of carcinogenicity of polycyclic aromatic hydrocarbons. 20143991677
more effects of cigarettes. 20144181171
visceral pain. 20143899257
late effects of malnutrition-mental aspects. 20144662223
[quality of the aortic wall and the degree of severity of atheroma]. 20144243815
endoscopic reduction of acute gastric volvulus complicating motor neuron disease. 20144019155
phenotypic and genotypic characterization of some lactic acid bacteria isolated from bee pollen: a preliminary study.in the present work, five hundred and sixty-seven isolates of lactic acid bacteria were recovered from raw bee pollen grains. all isolates were screened for their antagonistic activity against both gram-positive and gram-negative pathogenic bacteria. neutralized supernatants of 54 lactic acid bacteria (lab) cultures from 216 active isolates inhibited the growth of indicator bacteria. they were phenotypically characterized, based on the fermentation of 39 carbohydrates. using the simple matching ...201424936378
[myocardial infarct in the follow-up therapy]. 20144323334
isolation and characterization of faecal bifidobacteria and lactobacilli isolated from dogs and primates.although bifidobacteria and lactobacilli have been suggested beneficial for the host and are components of many probiotics and competitive exclusion mixtures, the knowledge on abundance, metabolic and probiotic characteristics in isolates from dogs and monkeys is still limited. the present study was aimed to isolate bifidobacterium and lactobacillus strains (faeces of 22 dogs and of 5 primates: cebus apella, eulemur fulvus, erythrocebus patas, macaca fascicularis, papio hamadryas) with the maldi ...201424239978
cholesterol-lowering potentials of lactic acid bacteria based on bile-salt hydrolase activity and effect of potent strains on cholesterol metabolism in vitro and in vivo.this study collected different probiotic isolates from animal and plant sources to evaluate the bile-salt hydrolase activity of probiotics in vitro. the deconjugation potential of bile acid was determined using high-performance liquid chromatography. hepg2 cells were cultured with probiotic strains with high bsh activity. the triglyceride (tg) and apolipoprotein b (apo b) secretion by hepg2 cells were evaluated. our results show that the bsh activity and bile-acid deconjugation abilities of pedi ...201425538960
antibacterial, anti-inflammatory and probiotic potential of enterococcus hirae isolated from the rumen of bos primigenius.in the present study bacterial strains were isolated from the rumen fluids of bos primigenius and investigated their in vitro probiotic properties with potent antibacterial activity and anti-inflammatory effects. 9 g positive bacterial isolates were obtained and three isolates could able to tolerate gastric conditions, high bile salt concentrations and exhibited significant bactericidal effect against the enteric pathogens vibrio cholera, enterococcus faecalis, enterobacter aerogens, pseudomonas ...201424609495
fermentative recovery of lipids and proteins from freshwater fish head waste with reference to antimicrobial and antioxidant properties of protein hydrolysate.effectiveness of fermentation using lactic acid bacteria (lab) for recovering lipids and proteins simultaneously from freshwater fish head (fwh) was evaluated. three different proteolytic lab (pediococcus acidilactici ncim5368, enterococcus faecium ncim5335 and pediococcus acidilactici fd3) isolated from fish processing wastes were employed in the fermentation process. the fermentation conditions involved 10 % (w/w) glucose, 2 % (w/w) nacl and 10 % (v/w) lab cultures at 37 °c. the process result ...201425190843
[administrative policies for the handicapped and public health. cooperation of rehabilitation centers and the universities]. 20144235975
[are there indications for sympatholysis using phenol?].a phenol injection of the lumbar sympathetic chain has been performed 45 times on 40 patients whose mean age was 78 years and whose poor general condition was a contra-indication to any kind of anesthesia. the results have been evaluated at one month and one year in 33 patients. among these patients, 21 had an available distal artery seen on the arteriography and 12 no distal artery. our results suggest that: the injection is not always easy, the rate of complications is important: 2 deaths (4.5 ...20144093724
[histological and electron microscopic studies on cutaneous amyloid deposition in nc mice]. 20144032825
[deep q waves in the ecg of children--an electro-, vector- and echocardiographic study].out of 9557 children we found 120 children (1,26%), who showed deep q-waves of greater than or equal to 0.4 mv in 3 consecutive ecg's at least in one of the leads i, avl, v4, v5 and v6. 70 children were investigated additionally by echocardiography, 45 by vectorcardiography according to frank. echocardiography revealed an increased septal thickness in 5 patients, a borderline thickness in 8, an increased septal/posterior wall ratio in 4. in the vectorcardiogram 51,1% of the patients had no q-wav ...20144047060
papillary carcinoma of the thyroid. a clinicopathologic study of 241 cases treated at the university of florence, italy.a clinicopathologic study of 241 cases of papillary thyroid carcinoma treated at the university of florence medical school, florence, italy is presented. the features of greatest prognostic value were patient's age at presentation, small tumor size, total encapsulation, extrathyroid extension, multicentricity, and presence of distant metastases. the prognosis of the disease was not influenced by the pattern of tumor growth, presence of solid areas, initial presence or subsequent development of c ...20143967175
[the beginnings of child psychotherapy--exemplified by the fate of an orphan girl]. 20143911191
[cumulative morbidity from birth to 10 years of age in the group of prague children with an extended follow-up]. 20155052065
chemical ionization mass spectrometry of organophosphorus insecticides. 20154412408
[effect of stearic acid on the degree of tubocurarine chloride extraction from aqueous solutions by organic solvents as dependent on the ph of the medium]. 20154774709
beclomethasone dipropionate steriod aerosol in treatment of perennial allergic asthma in children.thirty-one chronic perennial asthmatics aged from 2(1/2) to 16 years were treated with beclomethasone dipropionate pressurized aerosols for up to 20 months. of these, 16 patients dependent on oral corticosteroid or corticotrophin for up to 11 years were successfully transferred to this treatment, with one exception. steroid withdrawal symptoms were slight. loss of weight, disappearance of cushingoid features, and resumption of growth indicated lack of systemic side effects. fifteen others inadeq ...20154352726
[screening, combination of microbial deodorizer and the optimization of its deodorizing conditions].in order to obtain the candidate strains of deodorizing microorganism, three optimization strains with relatively higher deodorizing capacity were isolated from landfill leachate, which were given the labels of cc3, cc7, cc13 and cc16. according to the results of morphological observation, physiological and biochemical tests, 16s rdna sequence homology analysis, the strains cc3, cc7, cc13 and cc16 were identified as pediococcus acidilactici, bacillus megaterium, lactobacillus acidophilus and alc ...201525898673
short communication: no antimicrobial effects from one source of commercial dried distillers grains with solubles.because residual antibiotics in dried distillers grains with solubles (ddgs) could lead to inadvertent feeding of antibiotics to animals, the objective of our study was to determine if a commercial ddgs contained antibiotics. the ddgs used in a feeding study, and milk from cows fed the ddgs, were below the detection limits for at least 17 antibiotics. additionally, we evaluated if ddgs had any antimicrobial effect against salmonella typhimurium, listeria innocua, escherichia coli atcc 25922, sta ...201526454305
modulation of the intestinal microbiota and morphology of tilapia, oreochromis niloticus, following the application of a multi-species probiotic.the intestinal microbiota and morphology of tilapia (oreochromis niloticus) were investigated after the application of a multi-species probiotic containing lactobacillus reuteri, bacillus subtilis, enterococcus faecium and pediococcus acidilactici (aquastar(®) growout). tilapia (55.03 ± 0.44 g) were fed either a control diet or a probiotic diet (control diet supplemented with aquastar(®) growout at 5 g kg(-1)). after four and eight weeks, culture-dependent analysis showed higher levels of lactic ...201526115752
effect of dietary incorporation of a multi-strain probiotic on growth performance and health status in rainbow trout (oncorhynchus mykiss).dietary supplementation with a multi-strain probiotic containing bacillus subtilis, enterococcus faecium, pediococcus acidilactici and lactobacillus reuteri has been examined for its effects on growth performance, intestinal microbiota, non-specific immune response and antioxidant status of rainbow trout. three groups of sub-adult trout were stocked into experimental tanks. a commercial diet was used as control, while the other two groups received diets supplemented with the multi-strain probiot ...201525411048
clinical judgement and clinical research. 20155023227
[study of osteochondrosis]. 20155023269
bacterial diversity and mycotoxin reduction during maize fermentation (steeping) for ogi production.bacterial diversity and community structure of two maize varieties (white and yellow) during fermentation/steeping for ogi production, and the influence of spontaneous fermentation on mycotoxin reduction in the gruel were studied. a total of 142 bacterial isolates obtained at 24-96 h intervals were preliminarily identified by conventional microbiological methods while 60 selected isolates were clustered into 39 otus consisting of 15 species, 10 genera, and 3 phyla by 16s rrna sequence analysis. ...201526697001
hospital physician relations. 20154523826
problems of the single-parent family unit.case reports on single-parents families demonstrate some unique problems with which such a family unit must cope. single mothers frequently present children to the family physician, pediatrician or child psychiatrist with specific symptom complaints. there exists a need to recognize that these symptoms may reflect the special problems of the single-parent family or unresolved issues which led to the formation of the unit.to meet the needs of these parents the physician must explore the specific ...20154653933
Refresh
firstprev
123456789
nextlast
Displaying items 601 - 700 of 806