Publications

production of bacteriocins from lactococcus lactis subsp. lactis cect 539 and pediococcus acidilactici nrrl b-5627 using mussel-processing wastes.	the growth and bacteriocin production by lactococcus lactis subsp. lactis cect 539 and pediococcus acidilactici nrrl b-5627 were investigated on mussel-processing wastes. both bacteriocin productions were satisfactorily modelled using a modified form of the luedeking and piret expression, which includes a term for the influence of the ph reduction rate. experimental data from cultures buffered at different initial concentrations (0, 0.03, 0.10 and 0.25 m) of both bacteria were used to fit and ve ...	2002	12241553
multilocus hybridization typing in pediococcus acidilactici strains.	in previous a study we demonstrated the presence of several genomic subpopulations within a collection of pediococcus acidilactici strains isolated from different environments, through a multilocus typing analysis taking into consideration housekeeping conserved loci and protein coding genes of the primary metabolism. in this study, representative strains of five genomic subpopulations previously described (i, ii, iii, v, vii) were analyzed by restriction analysis of chromosomal dna and subseque ...	2002	11815849
cloning of the l-lactate dehydrogenase gene from the ruminal bacterium selenomonas ruminantium hd4.	a clone from a selenomonas ruminantium hd4 lambda zap ii genomic library was isolated by its ability to complement the anaerobic growth deficiency of an escherichia coli (pfl, ldh) double mutant. the 1.0-kb insert from the clone was sequenced and revealed a single open reading frame (orf, 957-bp) which was preceded by a putative shine-dalgarno (sd) sequence (aggggg). the potential sd sequence corresponded to 3' 16s rrna sequences of various selenomonas strains. the orf was predicted to encode a ...	2002	11821921
[group b vitamins in some typical italian cheese: parmigiano-reggiano, pecorino romano and gorgonzola (author's transl)].	the levels of vitamins b1, b2, b6, b12, pp and calcium pantothenate were determined in typical italian cheese: parmigiano-reggiano, pecorino romano and gorgonzola. the authors avaluated vitamin contents different from product; to product these differences being possibly due to both similar quantitative diversities of the milk, used and to particular variations associated with the processing techniques.	2002	134450
[critical studies on the evaluation of need for special schooling in the halle school for handicapped].		2002	130128
[histochemical changes in the morphologic structure of tissues in women with inflammatory diseases of the internal genital organs].		2002	124045
[problems of the double roles of the university department and mental hospital (author's transl)].		2002	124038
results of replacement of cardiac valves by homologous dura mater valves.	homologous dura mater valve was employed in a series of 533 patients in the period between january 1971 and may 1974. the dura mater was sterilized and preserved in 98 percent glycerol solution at room temperature. important data were the following (1) no significant pressure gradient through the vale at rest; (2) no bacterial endocarditis; (3) two cases of fungal endocarditis; (4) no degeneration or retraction of the leaflets; (5) no thrombus formation in the valve; and (6) no anticoagulants we ...	2002	123848
genetics of mental subnormality.		2002	126710
novel food-grade plasmid vector based on melibiose fermentation for the genetic engineering of lactococcus lactis.	the alpha-galactosidase gene (aga) and a gene coding for a putative transcriptional regulator from the laci/galr family (galr) of lactococcus raffinolactis atcc 43920 were cloned and sequenced. when transferred into lactococcus lactis and pediococcus acidilactici strains, aga modified the sugar fermentation profile of the strains from melibiose negative (mel(-)) to melibiose positive (mel(+)). analysis of gala mutants of l. lactis subsp. cremoris mg1363 indicated that the putative galactose perm ...	2002	12450840
bacteriocinogenic activity from starter cultures used in spanish meat industry.	thirty-nine bacterial strains, obtained from commercial starter cultures and commonly used by the meat industry in spain, have been examined for their ability to produce bacteriocins. fourteen (35%) of them showed antagonism against at least one of the indicator strains, by the agar spot test. the strains showing an inhibitory action against pathogenic indicator strains were identified as pediococcus acidilactici, lactobacillus curvatus, lactobacillus pentosus and lactobacillus plantarum, which ...	2002	22061417
[therapeutic aspects of the afferent loop syndrome].		2002	134399
[air pollution and respiratory diseases (author's transl)].		2002	126396
[letter: villous atrophy during certain chronic dermatoses. importance and pathogenic value].		2002	124053
acute traumatic losses of abdominal wall substance.		2002	123967
[double central projection of viiith cranial nerve in the frog (rana esculenta l.)].	in the frog we have cut the anterior or the posterior root of the viiith nerve between scarpa's ganglion and the medulla oblongata. the degenerations show that both roots project onto the ventral and the dorsal vestibular nuclei.	2002	126728
occurrence in wild strains of neurospora crassa of genes controlling genetic recombination.	each of the main laboratory wild stocks of n. crassa carries one of two alleles at the rec-1 and rec-2 loci and one of three at the rec-3 locus. the constitutions of the stocks are given in fig. 1. some of those conserved are evidently not the originals. the third rec-3 gene (rec-3l), found in lindegren a, controls recombination at the am-1 locus to a level between that of rec-3+ and rec-3, the relative levels being 1 : 8 : 25. at the his-2 locus rec-3l is indistinguishable from rec-3+ in its le ...	2002	126680
[proceedings: therapeutic possibilities of deshydroepiandrosterone in faetal hypotrophy (author's transl)].	the therapeutic effect of dhea-s has been studied in 15 cases of intra-uterine growth retardation. this double-blind study has not been able to demonstrate any difference between the effect dhea-s and of nacl on oestriol excretion, hpl values, and biparietal diameter. only the uterine growth seems to be affected by dhea-s. thus it can be concluded that dhea-s has no therapeutic effect on placental insufficiency.	2002	127542
letter: the degradation of soluble versus insoluble fibrin in vivo.		2002	129882
[research on a new dialytic strategy].		2003	262285
frequency of down syndrome in livebirths by single-year maternal age interval: results of a massachusetts study.	an analysis of rates of intra-state down syndrome livebirths to massachusetts residents by single-year maternal age interval in 1958-1965 inclusive was carried out. a gradual increase of rate of the down syndrome occurred from age 20 to about age 31, and a steeper increase thereafter. different regression equations were derived in the 20-31 and the 33-45 age group. the regression equations were ln y = 0.04515 x -1.45759 for those age 20-31 and ln y = 0.24302x-7.57870, for those age 33-45, where ...	2003	150062
helping the patient with low back pain.		2003	152404
[diagnostic problems of primary amyloidosis].	surgical liver biopsy specimen of a women aged 58 with "biliary complaints" showed amyloid deposits of unknown nature. after a nearly two-years-course of the disease the patient died of cardiac and renal failure. clinical findings and laboratory tests without abnormal serum globulins suggested primary amyloidosis. morphologically amyloid was present in the heart, tongue, striated muscles and in many parenchymatous organs and endocrine glands. by electron microscopy no difference was revealed bet ...	2003	152408
presensitization of potential kidney recipients detected by cell kinetic studies in the pooled mixed lymphocyte reaction.	an accelerated response in the pooled mixed lymphocyte reaction (pmlr) and/or the existence of cytotoxic antibodies indicates presensitization of potential kidney recipients. the absence of cytotoxic antibodies does not exclude presensitization, which can be demonstrated by a premature response in the pmlr. however, transfusions may also lead to impaired proliferation. in some patients, blood transfusions induce blocking activities. the possible mechanisms of this phenomenon are discussed. measu ...	2003	150079
phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16s ribosomal dna analysis.	a total of 161 low-g+c-content gram-positive bacteria isolated from whole-crop paddy rice silage were classified and subjected to phenotypic and genetic analyses. based on morphological and biochemical characters, these presumptive lactic acid bacterium (lab) isolates were divided into 10 groups that included members of the genera enterococcus, lactobacillus, lactococcus, leuconostoc, pediococcus, and weissella: analysis of the 16s ribosomal dna (rdna) was used to confirm the presence of the pre ...	2003	12514026
autolytic activity and pediocin-induced lysis in pediococcus acidilactici and pediococcus pentosaceus strains.	to evaluate the autolytic phenotype of pediococcus acidilactici and p. pentosaceus, the peptidoglycan hydrolases content and the effect of pediocin ach/pa-1 and autolysins on cell lysis.	2003	12631191
isolation, selection, and characterization of lactic acid bacteria for a competitive exclusion product to reduce shedding of escherichia coli o157:h7 in cattle.	lactic acid bacteria (lab) were selected on the basis of characteristics indicating that they would be good candidates for a competitive exclusion product (cep) that would inhibit escherichia coli o157:h7 in the intestinal tract of live cattle. fecal samples from cattle that were culture negative for e. coli o157:h7 were collected. lab were isolated from cattle feces by repeated plating on deman rogosa sharpe agar and lactobacillus selection agar. six hundred eighty-six pure colonies were isolat ...	2003	12636285
functional replacement of the escherichia coli d-(-)-lactate dehydrogenase gene (ldha) with the l-(+)-lactate dehydrogenase gene (ldhl) from pediococcus acidilactici.	the microbial production of l-(+)-lactic acid is rapidly expanding to allow increased production of polylactic acid (pla), a renewable, biodegradable plastic. the physical properties of pla can be tailored for specific applications by controlling the ratio of l-(+) and d-(-) isomers. for most uses of pla, the l-(+) isomer is more abundant. as an approach to reduce costs associated with biocatalysis (complex nutrients, antibiotics, aeration, product purification, and waste disposal), a recombinan ...	2003	12676706
influence of ph drop on both nisin and pediocin production by lactococcus lactis and pediococcus acidilactici.	to develop a kinetic model for describing the specific effect of ph drop on nisin and pediocin production in whey.	2003	12803556
substrate inhibition of pediococcus acidilactici by glucose on a waste medium. simulations and experimental results.	the possibility of substrate inhibition by glucose on biomass and pediocin production was studied in cultures of pediococcus acidilactici on a residual medium.	2003	14633105
characterization and heterologous expression of a class iia bacteriocin, plantaricin 423 from lactobacillus plantarum 423, in saccharomyces cerevisiae.	lactobacillus plantarum 423 produces a small heat-stable antimicrobial protein designated plantaricin 423. this protein is bactericidal for many gram-positive foodborne pathogens and spoilage bacteria, including listeria spp., staphylococcus spp., pediococcus spp., lactobacillus spp., etc. the dna sequence of the plantaricin 423-encoding region on plasmid ppla4 revealed a four open reading frame (orf) operon structure similar to pediocin pa-1/ach from pediococcus acidilactici and coagulin from b ...	2003	12423916
effects of indigenous starter cultures on the microbial and physicochemical characteristics of urutan, a balinese fermented sausage.	urutan is a balinese traditional dry fermented sausage prepared from lean pork and various kinds of spice. urutan is different from the european sausages, because it is fermented under warm condition with fluctuating temperatures of approximately 25 degrees c at night to 50 degrees c during sun drying. in this study, two of the 71 strains of lactic acid bacteria (lab) isolated from natural urutan fermentation were used as starter cultures: lactobacillus plantarum u201, the dominant lab, and pedi ...	2004	16233672
a rapid turbidometric microplate bioassay for accurate quantification of lactic acid bacteria bacteriocins.	a 1 day turbidometric microplate bioassay (tmb) was developed for the rapid, accurate and precise quantification of lactic acid bacteria (lab) bacteriocins (nisin z and pediocin pa-1). parameters such as the concentration of the indicator strains and the incubation time were optimized for each bacteriocin. a high correlation coefficient (r(2)=0.992+/-0.004) was obtained for the exponential regression in the nisin z concentration range of 20-120 ng/ml with 1 x 10(7) cfu indicator strain (pediococ ...	2004	14751683
pediocin production by pediococcus acidilactici in solid state culture on a waste medium: process simulation and experimental results.	the production of pediocin by pediococcus acidilactici was comparatively studied in submerged and solid-state culture, using polyurethane foam particles soaked in commercial (mrs) and waste media with various supplements, where product concentrations were 15 times higher in mrs medium. for the solid state analysis, cultures were treated by successive compression and refilling of tubular minireactors equipped with a piston, without the need for reinoculation. this method was found to be simple, r ...	2004	14966809
intra-specific variation of lactobacillus plantarum and lactobacillus pentosus in sensitivity towards various bacteriocins.	fifty-two strains belonging to the lactobacillus plantarum species group were identified and typed. they represented 32 clones of lactobacillus plantarum and 7 clones of lactobacillus pentosus. sensitivity of all strains towards bacteriocins of four different producer strains was investigated using a deferred inhibition test (dit). substantial intra-specific variation in sensitivity of clones was observed towards bacteriocinogenic lactic acid bacteria producing nisin ( lactococcus lactis atcc 11 ...	2004	15028870
genotypic analyses of lactobacilli with a range of tannase activities isolated from human feces and fermented foods.	a total of 77 tannase producing lactobacilli strains isolated from human feces or fermented foods were examined for their genotypic profiles and intensities of tannase production. with a pcr-based assay targeting reca gene, all strains except one isolate were assigned to either lactobacillus plantarum, l. paraplantarum, or l. pentosus whereas a 16/23s rdna targeted pcr-based assay identified all except 6 isolates (inclusive of the above one isolate) as one of the closely related species. subsequ ...	2004	15053327
isolation, identification, and selection of lactic acid bacteria from alfalfa sprouts for competitive inhibition of foodborne pathogens.	several studies have investigated the control of pathogens on alfalfa sprouts, and some treatments have been shown to be effective in reducing pathogen populations. however, control methods investigated thus far only provide pathogen control at a given point in the sprouting process and can affect germination. competitive inhibition of pathogens with lactic acid bacteria might provide pathogen control throughout the sprouting process and up to consumption. the purpose of this study was to isolat ...	2004	15151232
antimicrobial potential of immobilized lactococcus lactis subsp. lactis atcc 11454 against selected bacteria.	immobilization of living cells of lactic acid bacteria could be an alternative or complementary method of immobilizing organic acids and bacteriocins and inhibit undesirable bacteria in foods. this study evaluated the inhibition potential of immobilized lactococcus lactis subsp. lactis atcc 11454 on selected bacteria by a modified method of the agar spot test. l. lactis was immobilized in calcium alginate (1 to 2%)-whey protein concentrate (0 and 1%) beads. the antimicrobial potential of immobil ...	2004	15222547
comparative composition of bacteria in the human intestinal microflora during remission and active ulcerative colitis.	ulcerative colitis is a severe, relapsing and remitting disease of the human large intestine characterised by inflammation of the mucosa and submucosa. the main site of disease is the sigmoid/rectal region of the large bowel but the aetiology remains unknown. there is considerable evidence to indicate that the components of the resident colonic microflora can play an important role in initiation of the disease. the present study was aimed at characterising the faecal microflora of ulcerative col ...	2004	15460067
population dynamics of the constitutive biota of french dry sausages in a pilot-scale ripening chamber.	the population dynamic of constitutive biota on 84 samples belonging to two different types of french fermented dry sausages during the ripening process in a pilot-scale ripening chamber was investigated. samples were analyzed in three steps of their production: fresh product, first drying stage, and finished product. in addition, 180 strains of lactic acid bacteria were identified using a miniaturized biochemical procedure of characterization. in general, the number of lactic acid bacteria that ...	2004	15508649
how do toxins penetrate cells?		2004	492314
lung biopsy in sarcoidosis.		2004	421597
[course of alcoholic predelirium during treatment with piracetam: results of serial psychometric tests (author's transl)].	thirty-six male alcoholics in a mild predelirial state were studied with a series of psychometric tests (reaction time to light and sound, reaction to multiple sequential stimuli, apprehension and tachistoscopic appreciation test, achievement and concentration test analogous to the pauli test). the degree of psychometrically determined abnormalities pointed to serious cerebral lesions even in the mild predelirial stage and exceeded the expectations gained from clinical impression. the disorders ...	2004	446321
minnesota maverick: owen h. wangensteen.		2004	368357
ontogeny of thymus factor secretion.	the circulating thymic factor (tf) detected by 0-conversion assay has been followed up in ontogeny. serum tf evaluation in pregnant thymectomized mice and in new born mice indicates that tf secretion starts on day 12-13 of foetal life and has reached the adult's level at birth.	2005	1087546
[thrombotic occlusion and late lysis in the arteria subclavia lusoria dextra].	case history of a 45 years aged male patient with an aortic arch anomaly. thrombotic occlusion of the a. lusoria dextra with symptoms of a subclavian-steal syndrome and anoxia in the right hand. spontaneous lysis after 8 weeks is documentated by arteriography.	2005	1183296
management of pathological fractures at the mayo clinic.		2005	1234120
[ethnomedical and transcultural psychiatric aspects of migration].	in consideration of the current socioeconomical and political development in the world a dramatic increase of migration streams are expected. migrant families are always a new phenomenon on both sides in host countries. one important root of these specific problems are: culture-conditioned differences concerning value orientation between immigrants of several ethnics on one hand and the resident population on the other hand, but also among the immigrants of several ethnic groups themselves. thes ...	2005	1287629
inadvertent epidural injection of thiopental: a case report.		2005	1169034
[indications for surgery in endocarditis].		2005	545053
fed-batch pediocin production by pediococcus acidilactici nrrl b-5627 on whey.	cell growth and pediocin production by pediococcus acidilactici nrrl b-5627 on whey were compared by using batch fermentation and re-alkalized fed-batch fermentation. the batch fermentations were performed on dwg [dw (diluted whey) supplemented with 1% (w/v) glucose], dwye [dw supplemented with 2% (w/v) yeast extract] and dwgye (dw supplemented with 1% glucose plus 2% yeast extract) media. the fed-batch culture on dwye medium was fed with a mixture of concentrated whey (48 g of total sugars/l) s ...	2005	15574118
microbiological study of lactic acid fermentation of caper berries by molecular and culture-dependent methods.	fermentation of capers (the fruits of capparis sp.) was studied by molecular and culture-independent methods. a lactic acid fermentation occurred following immersion of caper berries in water, resulting in fast acidification and development of the organoleptic properties typical of this fermented food. a collection of 133 isolates obtained at different times of fermentation was reduced to 75 after randomly amplified polymorphic dna (rapd)-pcr analysis. isolates were identified by pcr or 16s rrna ...	2005	16332762
use of an alpha-galactosidase gene as a food-grade selection marker for streptococcus thermophilus.	the alpha-galactosidase gene (aga) of lactococcus raffinolactis atcc 43920 was previously shown to be an efficient food-grade selection marker in lactococcus lactis and pediococcus acidilactici but not in streptococcus thermophilus. in this study, we demonstrated that the alpha-galactosidase of l. raffinolactis is thermolabile and inoperative at 42 degrees c, the optimal growth temperature of s. thermophilus. an in vitro assay indicated that the activity of this alpha-galactosidase at 42 degrees ...	2005	15956297
production of pediocin pa-1 in the methylotrophic yeast pichia pastoris reveals unexpected inhibition of its biological activity due to the presence of collagen-like material.	expression of the peda gene from pediococcus acidilactici, coding for mature bacteriocin pediocin pa-1, was investigated using the yeast pichia pastoris to obtain larger quantities of pediocin to support additional studies, including structure-function research. following various cloning strategies, a km71h (mut(s)) strain was selected. a significant concentration (74 microg/ml) of extracellular recombinant pediocin was obtained but the pediocin showed no biological activity. supernatant fluids ...	2005	16023368
thermophilin 110: a bacteriocin of streptococcus thermophilus st110.	a screen of thermophilic lactic acid bacteria identified streptococcus thermophilus strain st110 as the putative producer of a bacteriocin with high level of activity against pediococci. thermophilin 110 was isolated from culture supernatant after 16 h of growth and partially purified by a chloroform extraction procedure. the bacteriocin inhibited the growth of several lactic acid bacteria and in the case of pediococcus acidilactici, it induced cell lysis with the concomitant release of od260-ab ...	2005	16049660
nucleotide sequence, structural organization and host range of prs4, a small cryptic pediococcus pentosaceus plasmid that contains two cassettes commonly found in other lactic acid bacteria.	the complete nucleotide sequence of the cryptic plasmid prs4 (3550 bp) from pediococcus pentosaceus rs4 was determined. sequence analysis revealed the presence of three open reading frames (orfs). the putative protein coded by orf 1 showed 93% identity with the mobilization protein of lactobacillus casei plasmid plc88 and 94% identity with a sequenced fragment of the mobilization protein of p. damnosus plasmid pf8801, suggesting a common origin for these three mobilization proteins. the putative ...	2005	16054305
identification of predominant lactic acid bacteria isolated from traditionally fermented vegetable products of the eastern himalayas.	gundruk, sinki and khalpi are lactic-fermented vegetable products of sikkim in india, and inziangsang is a fermented leafy vegetable product of nagaland and manipur in india. a total of 65 samples of gundruk (25), sinki (12), khalpi (25) and inziangsang (3) were analysed for microbial counts. the population of lactic acid bacteria (lab) as well as aerobic mesophilic counts were at the level of 10(7) cfu g(-1). yeasts were detected only in few samples of sinki and khalpi. no moulds were detected. ...	2005	16055218
an improved and simplified method for the large-scale purification of pediocin pa-1 produced by pediococcus acidilactici.	the bacteriocin pediocin pa-1 produced by pediococcus acidilactici pac 1.0 offers significant potential as a food preservative and as an antimicrobial agent in the medical area. however, low production yields and difficulties in obtaining significant amounts of pure pediocin pa-1 have limited, in part, its biochemical and physical characterization. in the present study, we describe a simple and more efficient purification strategy for pediocin pa-1. a hydrophobic interaction chromatography step ...	2006	16117726
development of a competitive exclusion product for poultry meeting the regulatory requirements for registration in the european union.	competitive exclusion treatment is able to increase the pathogen colonization resistance of day-old chicks by applying probiotic bacteria stabilizing the indigenous microflora. in order to develop a safe microbial feed additive, various bacterial strains were isolated out of the gastrointestinal tract of healthy chickens. one hundred twenty-one representatives were selected based on differences in whole-cell protein patterns and screened for antagonistic properties. five effective strains (pedio ...	2006	16676374
plasmid profile patterns and properties of pediococci isolated from caper fermentations.	a collection comprising 14 isolates of pediococcus pentosaceus and one pediococcus acidilactici from the fermentation of caper fruits was studied. all isolates showed very similar fermentation profiles and produced a limited number of exoenzymes. all isolates carried large plasmids of diverse sizes between 20 and 55 kb, while some also contained smaller plasmids between 10 and 16 kb. cluster analysis of plasmid profiles revealed four main groups with various degrees of similarities. all amino ac ...	2006	16715824
methylglyoxal bypass identified as source of chiral contamination in l(+) and d(-)-lactate fermentations by recombinant escherichia coli.	two new strains of escherichia coli b were engineered for the production of lactate with no detectable chiral impurity. all chiral impurities were eliminated by deleting the synthase gene (msga) that converts dihydroxyacetone-phosphate to methylglyoxal, a precursor for both l: (+)- and d: (-)-lactate. strain tg113 contains only native genes and produced optically pure d: (-)-lactate. strain tg108 contains the ldhl gene from pediococcus acidilactici and produced only l: (+)-lactate. in mineral sa ...	2006	16868860
assessment of antibiotic susceptibility within lactic acid bacteria strains isolated from wine.	susceptibility to 12 antibiotics was tested in 75 unrelated lactic acid bacteria strains of wine origin of the following species: 38 lactobacillus plantarum, 3 lactobacillus hilgardii, 2 lactobacillus paracasei, 1 lactobacillus sp, 21 oenococcus oeni, 4 pediococcus pentosaceus, 2 pediococcus parvulus, 1 pediococcus acidilactici, and 3 leuconostoc mesenteroides. the minimal inhibitory concentrations of the different antibiotics that inhibited 50% of the strains of the lactobacillus, leuconostoc a ...	2006	16876896
lethality of commercial whole-muscle beef jerky manufacturing processes against salmonella serovars and escherichia coli o157:h7.	thermal processes used in making whole-muscle beef jerky include a drying step, which may result in enhanced pathogen thermotolerance and evaporative cooling that reduce process lethality. several salmonellosis outbreaks have been associated with beef jerky. in this study, a standardized process was used to inoculate beef strips with five-strain cocktails of either salmonella serovars or escherichia coli o157:h7, to marinate the strips at ph 5.3 for 22 to 24 h at 5 degrees c, and to convert the ...	2006	16995510
determination of bacteriocin activity with bioassays carried out on solid and liquid substrates: assessing the factor "indicator microorganism".	successful application of growth inhibition techniques for quantitative determination of bacteriocins relies on the sensitivity of the applied indicator microorganism to the bacteriocin to which is exposed. however, information on indicator microorganisms' performance and comparisons in bacteriocin determination with bioassays is almost non-existing in the literature. the aim of the present work was to evaluate the parameter "indicator microorganism" in bioassays carried out on solid -agar diffu ...	2006	17032438
pediococcus stilesii sp. nov., isolated from maize grains.	a gram-positive, coccus-shaped, lactic acid bacterium, strain lmg 23082t, was isolated from steeped maize grains. the organism is homofermentative and produces d- and l-lactic acid from glucose. 16s rrna gene sequence analysis revealed that the organism belongs to the genus pediococcus, with pediococcus pentosaceus and pediococcus acidilactici as nearest neighbours. genotypic fingerprinting, whole-cell protein electrophoresis, dna-dna hybridizations and physiological and biochemical tests allowe ...	2006	16449434
enumeration and identification of pediococci in powder-based products using selective media and rapid pfge.	in this study, pediococci selective medium (psm) was evaluated for the enumeration of pediococcus acidilactici and pediococcus pentosaceus from probiotic animal feed and silage inoculants. psm is based on the complex basal medium mrs supplemented with cysteine hydrochloride, novobiocin, vancomycin, and nystatin. no significant change in electivity was observed when pediococci where recovered from culture or powder-based products following incubation at 37 degrees c under anaerobic conditions for ...	2006	15925418
effect of a bacteriocin produced by pediococcus acidilactici against listeria monocytogenes and clostridium perfringens on spanish raw meat.	the inhibitory effect of a bacteriocin, produced by pediococcus acidilactici, against listeria monocytogenes and clostridium perfringens on spanish raw meat surface, was evaluated by in situ assays. samples were incubated with the bacteriocin and then with a culture of the pathogenic bacteria. the treatment with 500, 1000 or 5000 bacteriocin units/ml (bu/ml) reduced the counts of l. monocytogenes after storage at 15°c during 72h by 1, 2 or 3 log cycles and with 1000 or 5000 bu/ml after storage a ...	2006	22061374
treatment of multiple intracranial aneurysms in the anterior circulation--case report.	a 69-year-old female underwent surgery for subarachnoid hemorrhage due to a ruptured intracranial aneurysm. nine additional aneurysms were found. four of the latter nine aneurysms were clipped in a second operation, and two were wrapped.	2006	1694273
effects of low levels of lead exposure.		2006	1489377
capture, mutate, quantitate: variations of a central pcr scheme.		2006	1472383
[the comprehensive diagnosis and treatment of lymphosarcoma of the pharynx].	modern methods (intrascopic and other instrumental) were used to increase the diagnostic efficiency and extension of the tumour process in 22 patients with lymphosarcoma of the pharynx. optimal treatment efficacy is achieved in lymphosarcoma of the pharynx by combined use of surgical, radiation (non-traditional course) treatment as well as chemotherapy which is used at early stages as an adjunctive method.	2006	1448998
promoting sexual health.		2006	1392896
topical puva therapy for vitiligo.	vitiligo, a pigmentary disorder of the skin, causes the appearance of white patches which are profoundly devastating to the person afflicted with this disease. this article outlines the safe use of topical puva therapy in the office for treating those patients with less than 20% skin surface involvement. this treatment has a response rate of approximately 50%.	2006	1828681
moderate and mild mental retardation in the martin-bell syndrome.	a survey of children attending schools for the moderately or the mildly mentally handicapped has shown that two out of 25 boys and two out of 22 girls with idiopathic moderate mental retardation had the martin-bell syndrome, while none of 75 boys and one out of 51 girls with mild mental retardation were fraxa positive. consideration of these figures along with published studies suggests that 7% of moderate and 3.8% of mild idiopathic mental retardation in boys, and 2.5% of moderate and 3.3% of m ...	2006	1800751
moderate and mild mental retardation in the martin-bell syndrome.	a survey of children attending schools for the moderately or the mildly mentally handicapped has shown that two out of 25 boys and two out of 22 girls with idiopathic moderate mental retardation had the martin-bell syndrome, while none of 75 boys and one out of 51 girls with mild mental retardation were fraxa positive. consideration of these figures along with published studies suggests that 7% of moderate and 3.8% of mild idiopathic mental retardation in boys, and 2.5% of moderate and 3.3% of m ...	2006	1800751
[familial juvenile nephronophthisis].		2007	2075033
long-term outcome after corneal transplantation. visual result and patient perception of success.	snellen acuity, reading line, and keratometry were measured in a cohort of 60 patients at 2 or more years after penetrating keratoplasty was performed. patients were asked to complete a questionnaire to elicit information on their perceptions of visual function and the success of the procedure. using preferred correction, a snellen acuity of 6/18 or better was achieved by 65%, and a reading line of n8 or better was achieved by 57% of index grafts. thirty-eight percent had more than 5 diopters (d ...	2007	2062497
violent trends.		2007	2034554
infectious and genetic manifestations of prion diseases.		2007	1932814
effects of starter cultures and nitrite levels on formation of biogenic amines in sucuk.	the effects of starter cultures, starter a (lactobacillus sakei+staphylococcus carnosus) and starter b (pediococcus acidilactici+staphylococcus xylosus+lactobacillus curvatus), nitrite levels (0, 75, and 150ppm) and ripening period on biogenic amine production were investigated in sucuk (turkish dry-fermented sausage) production. changes in biogenic amines, ph, water activity, moisture, non-protein nitrogen, nitrite and counts of lactic acid bacteria, micrococcus/staphylococcus, enterobacteriace ...	2007	22061796
residual effectiveness of insecticides for control of german cockroaches (dictyoptera: blattellidae) in food-handling establishments.	residual effectiveness of insecticides was compared for two treatment methods at various sites within two commercial kitchens. for all sites and insecticides, german cockroach, blattella germanica (l.), mortality was greatest for treatments of cracks and crevices than for treatments of exposed surfaces. mortality at the dishwashing site was lower than at all other sites. we attributed reduced mortality at the dishwashing site to a higher vapor pressure deficit. mortality and the amount of insect ...	2007	2258513
peoc01: a plasmid from pediococcus acidilactici which encodes an identical streptomycin resistance (aade) gene to that found in campylobacter jejuni.	the complete nucleotide sequence of peoc01, a plasmid (11,661 bp) from pediococcus acidilactici ncimb 6990 encoding resistance to clindamycin, erythromycin, and streptomycin was determined. the plasmid, which also replicates in lactococcus and lactobacillus species contains 16 putative open reading frames (orfs), including regions annotated to encode replication, plasmid maintenance and multidrug resistance functions. based on an analysis the plasmid replicates via a theta replicating mechanism ...	2007	17395262
effects of pediococcus- and saccharomyces-based probiotic (mitomax) on coccidiosis in broiler chickens.	coccidiosis is the major parasitic disease of poultry. in this study, the role of the commercial probiotic mitomax which contains pediococcus acidilactici and saccharomyces boulardii was evaluated by measuring body weight gain, fecal oocyst shedding, and serum antibody responses as an alternative control method of prophylactic drug against coccidiosis. day-old broiler chicks were fed regular or probiotic diets supplemented with mitomax at 0.01%, 0.1%, or 1.0% of diet, and challenged 2 weeks late ...	2007	17434586
development of thermal surrogate microorganisms in ground beef for in-plant critical control point validation studies.	in search of a suitable surrogate microorganism for in-plant critical control point validation, we compared the rates of thermal inactivation of three bacteria, enterococcus faecium b2354, pediococcus parvulus hp, and pediococcus acidilactici lp, to those of listeria monocytogenes and salmonella. ground beef samples containing 4 and 12% fat were inoculated with e. faecium, l. monocytogenes, and salmonella senftenberg 775w and heated at 58, 62, 65, or 68 degrees c. the decimal reduction times (d- ...	2007	17477266
lactic acid bacteria and yeasts associated with gowé production from sorghum in bénin.	to identify the dominant micro-organisms involved in the production of gowé, a fermented beverage, and to select the most appropriate species for starter culture development.	2007	17650194
detection of the pediocin gene peda in strains from human faeces by real-time pcr and characterization of pediococcus acidilactici uva1.	bacteriocin-producing lactic acid bacteria are commonly used as natural protective cultures. among them, strains of the genus pediococcus are particularly interesting for their ability to produce pediocin, a broad spectrum antimicrobial peptide with a strong antagonistic activity against the food-borne pathogen listeria monocytogenes. furthermore, there is increasing interest in isolating new bacteriocin-producing strains of human intestinal origin that could be developed for probiotic effects a ...	2007	17850651
the biodiversity of predominant lactic acid bacteria in dolo and pito wort for the production of sorghum beer.	to quantify and identify the predominant lactic acid bacteria (lab) in dolo and pito wort processing, and to examine their biodiversity at strain level.	2007	17897178
chimeras of mature pediocin pa-1 fused to the signal peptide of enterocin p permits the cloning, production, and expression of pediocin pa-1 in lactococcus lactis.	chimeras of pediocin pa-1 (peda-1), a bacteriocin produced by pediococcus acidilactici plbh9, fused to the signal peptide of enterocin p (entp), a sec-dependent bacteriocin produced by enterococcus faecium p13, permitted the production of peda-1 in lactococcus lactis. chimeric genes encoding the entp signal peptide (sp(entp)) fused to mature peda-1 (peda), with or without its immunity gene (pedb), were cloned into the expression vector pmg36c to generate the recombinant plasmids pmpp9 (sp(entp): ...	2007	18095432
biological and genetic classification of canine intestinal lactic acid bacteria and bifidobacteria.	to investigate the distribution of lactic acid bacteria (lab) inhabiting canine intestines, a total of 374 gram-positive lab and bifidobacteria (bf) isolated from large intestinal contents in 36 dogs were classified and identified by phenotypic and genetic analyses. based on cell morphological sizes, these isolates were divided into seven biotypes containing the genera lactobacillus, bifidobacterium, enterococcus, and streptococcus. the lab and bf isolates were classified into 38 chemotypes base ...	2007	17951981
characterization and selection of autochthonous lactic acid bacteria isolated from traditional iberian dry-fermented salchichón and chorizo sausages.	a total of 192 lactic acid bacteria were isolated from 2 types of naturally fermented dry sausages at 3 different stages of the ripening process in order to select the most suitable strains as starter cultures in dry-cured sausage manufacture according to their technological characteristics such as glucose fermentation, lactic and acetic acid production, and proteolytic, lipolytic, and antimicrobial activities. identification of the isolates revealed that 31.2% were pediococcus pentosaceus, 26.9 ...	2007	17995686
production of active pediocin pa-1 in escherichia coli using a thioredoxin gene fusion expression approach: cloning, expression, purification, and characterization.	antimicrobial peptides possess cationic and amphipathic properties that allow for interactions with the membrane of living cells. bacteriocins from lactic acid bacteria, in particular, are currently being studied for their potential use as food preservatives and for applications in health care. however, bacteriocin exploitation is often limited owing to low production yields. gene cloning and heterologous protein or peptide production is one way to possibly achieve overexpression of bacteriocins ...	2007	18026219
morphological changes induced in listeria monocytogenes v7 by a bacteriocin produced by pediococcus acidilactici.	pediococcus acidilactici produces bacteriocin, which kills listeria monocytogenes. the bactericidal mode of action of the bacteriocin against l. monocytogenes v7 was investigated by transmission electron microscopy. the bacteriocin was purified partially from the cell-free extract using micro-cel and cation-exchange chromatography, and the specific activity was increased 1,791 fold. the bacteriocin (6,400 au/ ml) was inoculated with l. monocytogenes v7 and incubated for 0.5 h, 1 h, 3 h, and 6 h. ...	2007	18051280
antimicrobial susceptibility of non-enterococcal intrinsic glycopeptide-resistant gram-positive organisms.	non-enterococcal gram-positive bacteria that are intrinsically vancomycin-resistant have been infrequently isolated in association with serious infections. however, well-documented infections have lately been reported with increasing frequency. because these organisms may be pathogens, we tested the mics of 19 antimicrobial agents by the agar dilution method for predicting susceptibility. the activity of these antimicrobial agents was assessed against 28 strains (lactobacillus rhamnosus, 6; lact ...	2007	17049799
modification of the data-processing method for the turbidimetric bioassay of nisin.	the data processing method of the turbidimetric bioassay of nisin was modified to facilitate its industrial application. the influence of the initial indicator concentration was minimized by a redefined specific dose of the bacteriocin as the quotient between the titer of the added bacteriocin and the initial population density of the indicator in the suspension. it was found that dc = 0.125 microg ml(-1) was the critical dose of nisin that can cause a complete inhibition of the indicator, pedio ...	2007	17063329
influence of pediococcus-based probiotic on coccidiosis in broiler chickens.	coccidiosis is the major parasitic disease of poultry and is caused by the apicomplexan parasites eimeria. drugs and live vaccines are the 2 main control measures of the disease; however, due to increasing concerns with prophylactic drug use and the high cost of vaccines, alternative control methods are needed. recent evidence that various dietary and live microbial supplements can influence host immunity against enteric diseases prompted us to investigate the role of a pediococcus-based probiot ...	2007	17179417
partial characterization of bacteriocins produced by human lactococcus lactis and pediococccus acidilactici isolates.	the aim of this study was to isolate bacteriocin-producing lactic acid bacteria (lab) from human intestine.	2007	17184344
susceptibility of pediococcus spp. to antimicrobial agents.	to investigate the susceptibility of pediococcus species to antimicrobial agents.	2007	17241343
limitations in the use of drosophila melanogaster as a model host for gram-positive bacterial infection.	to examine sensitivities of various drosophila melanogaster strains towards human pathogenic and nonpathogenic gram-positive bacteria.	2007	17257264
optimization of technical conditions for the transformation of pediococcus acidilactici p60 by electroporation.	previously reported techniques for the electrotransfer of foreign dna into pediococci yield only a small number of transformants/mug dna, especially when using undomesticated strains. this study reports an improved protocol for the electrotransformation of pediococci, based on trials using pediococcus acidilactici p60 and the plasmid prs4c1. the improved protocol yields from 2 to 3 log units more transformants than the previously reported methods, with up to (9.1+/-1.3)x10(4) transformants/mug o ...	2007	17275085
characterization of two bacteriocins produced by pediococcus acidilactici isolated from "alheira", a fermented sausage traditionally produced in portugal.	lactic acid bacteria were isolated from "alheira" sausages that have been sampled from different regions in portugal. the sausages were produced according to different recipes and with traditional starter cultures. two isolates (ha-6111-2 and ha-5692-3) from different sausages were identified as strains of pediococcus acidilactici. each strain produces a bacteriocin, designated as bacha-6111-2 and bacha-5692-3. both bacteriocins are produced at low levels after 18 h of growth in mrs broth (3200 ...	2007	17368595