Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| complete nucleotide sequence of psmb 74, a plasmid encoding the production of pediocin ach in pediococcus acidilactici. | several pediococcus acidilactici strains produce a plasmid-encoded bacteriocin, pediocin ach. previous studies have shown that this plasmid, designated as psmb 74, encodes genes associated with the production of prepediocin, its post-translation processing to pediocin ach, transmembrane translocation of these molecules, and immunity of producer cells against pediocin ach. we report here the complete nucleotide sequence of psmb 74. the plasmid has a total of 8877 bp. four genes have been located ... | 1994 | 7764941 |
| analysis of the pediocin ach gene cluster from plasmid psmb74 and its expression in a pediocin-negative pediococcus acidilactici strain. | the 3,500-bp pap operon in the 8,877-bp plasmid psmb74 contains a cluster of four genes, papabcd, of which papa encodes prepediocin (a. m. motlagh, m. bukhtiyarova, and b. ray, lett. appl. microbiol. 18:305-312, 1994). the cluster without the promoter was cloned in the shuttle vector phps9. an escherichia coli strain and a pediocin-sensitive pediococcus acidilactici strain transformed with the recombinant plasmid, pmbr1.0, produced pediocin ach. deletion analysis by introducing mutations in the ... | 1994 | 7944372 |
| [microbiological and clinical studies of vancomycin resistant leuconostoc spp. and pediococcus spp. isolated from septicemia patients]. | we described three septicemia cases in which blood cultures yielded gram-positive cocci identified as leuconostoc spp. and pediococcus spp. patients were three male adults aged 63 to 71 years with severe underlying diseases, pancreatic cancer, esophageal cancer and diabetes mellitus with chronic renal failure. they had fever and chills at the onsets of septicemia with acute obstructive suppurative cholangitis, acute pneumonia, and infection complicated with invasion sites of esophageal cancer co ... | 1994 | 7963799 |
| monoclonal antibody-based enzyme immunoassay for pediocins of pediococcus acidilactici. | monoclonal antibody (mab) r2-ar against pediocin rs2 was developed. mice were immunized for 12 weeks with pediocin rs2 conjugated to a polyacrylamide gel. two hybridoma fusions yielded an mab that in western blots (immunoblots) reacted only with pediocins rs2 and ach (3 kda) from pediococcus acidilactici rs2 and h, respectively, and did not react with any other bacteriocin, including sakacin a from lactobacillus sake lb 706, leuconocin lcm1 from leuconostoc carnosum lm1, nisin from lactococcus l ... | 1994 | 8085814 |
| influence of ph, salt and nitrite on the heme-dependent catalase activity of lactic acid bacteria. | a screening of commercial starter cultures used for the production of dry sausage showed a maximum heme-dependent catalase activity in the range of 60 mumol/l hematin for lactobacillus sake, lactobacillus plantarum, lactobacillus pentosus and pediococcus acidilactici. pseudocatalase activity was not detected. in standard dry sausage production, 2-3% (w/w) nitrite salt (0.6% sodium nitrite per 100 g nacl) is normally added, which corresponds to 4-6% salt in the water phase. in vitro experiments w ... | 1994 | 7703013 |
| simple method of purification and sequencing of a bacteriocin produced by pediococcus acidilactici ul5. | a bacteriocin produced by a strain of pediococcus acidilactici was successfully purified sequentially by acid extraction (at ph 2) and reverse-phase high-performance liquid chromatography (hplc). cell extracts of derivative strains deficient in bacteriocin production exhibited a similar hplc elution profile to the active extracts except for the two peaks containing bacteriocin activity. the sequence of the antibacterial peptide consisted of 44 amino acid residues of which 42 were identified, and ... | 1994 | 7822227 |
| cloning, nucleotide sequence, and transcriptional analysis of the pediococcus acidilactici l-(+)-lactate dehydrogenase gene. | recombinant plasmids containing the pediococcus acidilactici l-(+)-lactate dehydrogenase gene (ldhl) were isolated by complementing for growth under anaerobiosis of an escherichia coli lactate dehydrogenase-pyruvate formate lyase double mutant. the nucleotide sequence of the ldhl gene predicted a protein of 323 amino acids showing significant similarity with other bacterial l-(+)-lactate dehydrogenases and especially with that of lactobacillus plantarum. the ldhl transcription start points in p. ... | 1995 | 7887607 |
| isolation and characterization of pediocin l50, a new bacteriocin from pediococcus acidilactici with a broad inhibitory spectrum. | lactic acid bacteria were isolated from spanish dry-fermented sausages and screened for bacteriocin production. about 10% of the isolates produced antimicrobial substances when grown on solid media, but only 2% produced detectable activity in liquid media. strain l50, identified as pediococcus acidilactici, showed the strongest inhibitory activity and was active against members of all of the gram-positive genera tested. the strain produced a heat-stable bacteriocin when grown at 8 to 32 degrees ... | 1995 | 7618877 |
| pediococcus acidilactici ldhd gene: cloning, nucleotide sequence, and transcriptional analysis. | the gene encoding d-lactate dehydrogenase was isolated on a 2.9-kb insert from a library of pediococcus acidilactici dna by complementation for growth under anaerobiosis of an escherichia coli lactate dehydrogenase and pyruvate-formate lyase double mutant. the nucleotide sequence of ldhd encodes a protein of 331 amino acids (predicted molecular mass of 37,210 da) which shows similarity to the family of d-2-hydroxyacid dehydrogenases. the enzyme encoded by the cloned fragment is equally active on ... | 1995 | 7539419 |
| expression of lactococcin a and pediocin pa-1 in heterologous hosts. | pediocin pa-1 production, immunity and secretion are specified by a cluster of four genes in pediococcus acidilactici pac1.0. the production by, secretion of, and immunity to lactococcin a of lactococcus lactis are also determined by four genes. here, expression of the pediocin operon in lactococcus lactis is reported, which could only be achieved by placing it under control of a lactococcal promoter. expression of the lactococcin a operon in pediococcus is also described: recombinant clones of ... | 1995 | 7576505 |
| functional analysis of the pediocin operon of pediococcus acidilactici pac1.0: pedb is the immunity protein and pedd is the precursor processing enzyme. | the bacteriocin pediocin pa-1 operon of pediococcus acidilactici pac1.0 encompasses four genes: peda, pedb, pedc and pedd. transcription of the operon results in the formation of two overlapping transcripts, probably originating from a single promoter upstream of peda. the major transcript comprises peda, pedb, and pedc, while a minor transcript encompasses all of these genes and pedd. by deletion analysis and overexpression of pedb in pediococcus pentosaceus we demonstrate that this gene encode ... | 1995 | 8559070 |
| production of pediocin ach by lactobacillus plantarum whe 92 isolated from cheese. | among 1,962 bacterial isolates from a smear-surface soft cheese (munster cheese) screened for activity against listeria monocytogenes, six produced antilisterial compounds other than organic acids. the bacterial strain whe 92, which displayed the strongest antilisterial effect, was identified at the dna level as lactobacillus plantarum. the proteinaceous nature, narrow inhibitory spectrum, and bactericidal mode of action of the antilisterial compound produced by this bacterium suggested that it ... | 1996 | 8953710 |
| pediocin 5 production and plasmid stability during continuous free and immobilized cell cultures of pediococcus acidilactici ul5. | continuous production of pediocin 5 from pediococcus acidilactici ul5 was investigated in mrs medium at different ph and dilution rates during continuous free cell (fc) and immobilized cell (ic) fermentations. pediocin 5 activity from fc operated at a dilution rate of 0.31 h-1 largely increased from 128 to 2048 au ml-1 as ph decreased from 7.0 to 5.0. pediocin 5 activity in ic at a dilution rate of 0.93 h-1 was much less affected by ph, varying from 256 au ml-1 at ph 7.0 to 512 au ml-1 at ph 5.0 ... | 1996 | 8698665 |
| development and characterization of lactose-positive pediococcus species for milk fermentation. | bacteriophages against streptococcus thermophilus are a growing problem in the italian cheese industry. one possible control method involves replacing s. thermophilus in mozzarella starter blends with lactic acid bacteria from a different genus or species. in this study, we evaluated lactose-positive pediococci for this application. because we could not identify any commercially available pediococci with fast acid-producing ability in milk, we transformed pediococcus pentosaceus atcc 25744, p. p ... | 1996 | 16535280 |
| identification of pediococcus acidilactici and pediococcus pentosaceus based on 16s rrna and ldhd gene-targeted multiplex pcr analysis. | 1997 | 9228758 | |
| rapid purification, partial characterization, and antimicrobial spectrum of the bacteriocin, pediocin acm, from pediococcus acidilactici m. | the bacteriocin from pediococcus acidilactici m, designated as pediocin acm, was rapidly purified to homogeneity by the ph mediated cell adsorption-desorption method and semi-preparative reversed-phase hplc. the purification yield was 40.4% and the specific activity was increased by 2450-fold. it gave a single band and a single peak on sds-page and hplc analysis, respectively. when subjected to electrospray lc-ms analysis, the protein was found to be highly pure and the molecular weight was dete ... | 1997 | 9237116 |
| pediocin pd-1, a bactericidal antimicrobial peptide from pediococcus damnosus ncfb 1832. | pediocin pd-1, produced by pediococcus damnosus ncfb 1832, is inhibitory to several food spoilage bacteria and food-borne pathogens. however, pediocin pd-1 is not active against other pediococcus spp. and differs in this respect to other pediocins produced by pediococcus acidilactici and pediococcus pentosaceus. production of pediocin pd-1 starts during early growth and reaches-a plateau at the end of exponential growth. pediocin pd-1 was partially purified and its size was determined by tricine ... | 1997 | 9246779 |
| characterization of the region downstream of the pilus biogenesis gene pilc1 in neisseria gonorrhoeae. | the nucleotide sequence of a 3 kb region downstream of pilc1 in neisseria gonorrhoeae ms11 was analyzed. this region contains two open reading frames, orf1 and orf2, and several repetitive dna elements. orf1 encodes an outer membrane protein that shows homology to orf98 of pediococcus acidilactici. pcr with primers specific for orf1 revealed that the gene is present in all gonococcal strains tested. the other open reading frame, orf2, is highly homologous to the putative integral membrane protei ... | 1998 | 9565669 |
| pediococcus acidilactici pneumonitis and bacteremia in a pregnant woman. | 1998 | 9675696 | |
| discrimination among pediocin ach/pa-1 producer strains by comparison of pedb and pedd amplified genes and by multiplex pcr assay. | pediococcus acidilactici, pediococcus parvulus and lactobacillus plantarum pediocin ach/pa-1 produces strains were studied with the aim to investigate their common genetic pediocin determinant using peda, pedb, pedc and pedd gene-targeted pcr assay. single strand conformation polymorphism and restriction analysis of peda and pedc amplified fragments from the three different species did not show any differences in sequence while these analysis carried out on pedb and pedd amplified fragments high ... | 1998 | 9841130 |
| isolation, purification and partial characterization of plantaricin 423, a bacteriocin produced by lactobacillus plantarum. | lactobacillus plantarum 423, isolated from sorghum beer, produces a bacteriocin (plantaricin 423) which is inhibitory to several food spoilage bacteria and food-borne pathogens, including bacillus cereus, clostridium sporogenes, enterococcus faecalis, listeria spp. and staphylococcus spp. plantaricin 423 is resistant to treatment at 80 degrees c, but loses 50% of its activity after 60 min at 100 degrees c and 75% of its activity after autoclaving (121 degrees c, 15 min). plantaricin 423 remains ... | 1998 | 9717299 |
| generation of polyclonal antibodies of predetermined specificity against pediocin pa-1. | polyclonal antibodies of predetermined specificity for pediocin pa-1 (peda1) have been generated by immunization of rabbits with a chemically synthesized c-terminal fragment of this bacteriocin (ph2) conjugated to the carrier protein keyhole limpet hemocyanin (klh). the sensitivity and specificity of the ph2-klh-generated antibodies were evaluated by the development of various enzyme-linked immunosorbent assays (elisas)-a noncompetitive indirect elisa (nci-elisa), a competitive indirect elisa (c ... | 1998 | 9797320 |
| isolation and characterization of pediocin ach chimeric protein mutants with altered bactericidal activity. | a collection of pediocin ach amino acid substitution mutants was generated by pcr random mutagenesis of dna encoding the bacteriocin. mutants were isolated by cloning mutagenized dna into an escherichia coli male plasmid that directs the secretion of maltose binding protein-pediocin ach chimeric proteins and by screening transformant colonies for bactericidal activity against lactobacillus plantarum ncdo955 (k. w. miller, r. schamber, y. chen, and b. ray, 1998. appl. environ. microbiol. 64:14-20 ... | 1998 | 9603806 |
| production of pediocin pa-1 by lactococcus lactis using the lactococcin a secretory apparatus. | the class ii bacteriocins pediocin pa-1, from pediococcus acidilactici, and lactococcin a, from lactococcus lactis subsp. lactis bv. diacetylactis wm4 have a number of features in common. they are produced as precursor peptides containing similar amino-terminal leader sequences with a conserved processing site (gly-gly at positions -1 and -2). translocation of both bacteriocins occurs via a dedicated secretory system. because of the strong antilisterial activity of pediocin pa-1, its production ... | 1998 | 9501421 |
| binding of heterocyclic amines by lactic acid bacteria from miso, a fermented japanese food. | miso, a widely used japanese fermented food was analysed for its lactic acid bacterial count on bromocresol purple agar. the binding of eight different foodborne carcinogenic heterocyclic amines to 25 bacterial isolates from miso were investigated. the heterocyclic amines used were 3-amino-1,4-dimethyl[5h]pyrido(4,3-b)indole (trp-p-1), 3-amino-1-methyl[5h]pyrido(4,3-b)indole (trp-p-2), 2-amino-6-methyldipyrido(1,2-a:3'2'-d)imidazole (glu-p-1), 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (phi ... | 1998 | 9543712 |
| a sad commentary, a happy ending. | 1998 | 106198 | |
| endotoxin and the inflammatory process. | 1998 | 106332 | |
| [the behavior of bioelements during long-term parenteral feeding]. | 26 adult patients were investigated for 26--35 postoperative days while being fed parenterally. serum levels and urine excretion of zinc, magnesium, calcium and phosphate were measured. from the results it can be assumed that only phosphate substitution is necessary during parenteral long-term nutrition. | 1998 | 107121 |
| the child with epilepsy. valproic acid for children with uncontrolled epilepsy. | 1998 | 106203 | |
| bacterial meningitis. | 1998 | 90938 | |
| intraventricular hemorrhage and hydrocephalus in premature newborns: a prospective study with ct. | in a prospective study, 100 premature infants were studied with computed tomography (ct) brain scans within the first week of life. in 44 of these,hemorrhages originated from the subependymal germinal matrix, and ranged in severity from isolated germinal matrix hemorrhages to blood-filled, dilated ventricles with extension of hemorrhage into the brain parenchyma. a system of grading the severity of hemorrhage was developed. grades i and ii hemorrhages resolved spontaneously and grades iii and iv ... | 1998 | 106697 |
| what goes on under your make-up if you are allergic to cosmetics. | 1998 | 124665 | |
| the conservative management of anorectal cancer by radiotherapy. | thirteen patients with an anal or rectal carcinoma were given curative radiotherapy. four had medically inoperable tumors, one had a surgically inoperable tumor and eight refused abdominoperineal resection. six patients received external radiotherapy only. seven patients received external radiotherapy and an interstitial implant. nine of thirteen patients (60 per cent) are alive without evidence of disease from fifteen to fifty-five months (average, 30 months). six of seven patients who received ... | 1998 | 106741 |
| ultrasound demonstration of diffuse cortical nephrocalcinosis in a case of primary hyperoxaluria. | 1998 | 106704 | |
| [akinetic seizures in adult life (author's transl)]. | 1998 | 106311 | |
| [operative trauma of the common bile duct. report of eight cases (author's transl)]. | the authors report eight cases of operative trauma to the common bile duct; seven patients were sent up for reoperation often during the late stages following an attempt at immediate or secondary repair. emphasis was placed on the interest of extensive resection of the stenosis and wide and high opening of the common bile duct for construction of the bilio-jejunal anastomosis, and furthermore the late risk of onset of portal hypertension requiring portacaval anastomosis, finally the possible deg ... | 1998 | 106063 |
| [dosimeter for radiation protection. din 6818. sheet 1. general rules]. | 1998 | 124699 | |
| the diet/heart controversy. | 1998 | 90932 | |
| splenic flexure volvulus: two case reports and review. | 1998 | 106660 | |
| [a criteria for clinical evaluation of antibiotics on symptoms of bacillary dysentery. ii. severity of course (author's transl)]. | 1998 | 105061 | |
| nephrotic syndrome and cardiovascular disease. | 1999 | 91743 | |
| [current methods of treating generalized and disseminated forms of lymphogranulomatosis]. | 1999 | 89613 | |
| cardiac arrhythmia and haloperidol. | 1999 | 91324 | |
| combination chemotherapy with cis-dichlorodiammineplatinum(ii) in disseminated testicular cancer. | 1999 | 91434 | |
| peritoneal drainage. | 1999 | 91029 | |
| [chemotherapy of testicular neoplasms]. | 1999 | 91476 | |
| increased frequency of hla--dw3 in north-american black patients with juvenile onset diabetes. | 1999 | 91214 | |
| [the use of beta-galactosidase in technology of food preparation (author's transl)]. | 1999 | 99769 | |
| chemotherapy of head and neck cancer. | chemotherapy has traditionally been relegated to a late palliative role in the management of head and neck cancer. for advanced disease in patients previously treated with surgery or radiation therapy, methotrexate, which has a response rate of approximately 50%, is the best single agent available. other orally or systemically administered single agents--such as bleomycin, adriamycin, hydroxyurea, and cis-platinum--produce responses less frequently than methotrexate. intraarterial chemotherapy w ... | 1999 | 91591 |
| regulatory role of suppressor t cells in the expression of delayed-type hypersensitivity in mice. ii. soluble factor from thymic suppressor cells stimulated with antigen in vitro and its possible interaction with macrophages. | thymus cells from mice primed s.c. with a high dose (10 mg) of lysozyme (lys) specifically suppressed delayed footpad reaction (fpr) in mice previously immuned with lipid-conjugated lysozyme (d.lys), and also suppressed the transfer of fpr by d.lys-immune spleen cells into normal mice. furthermore, they inhibited antigen-stimulated dna synthesis of d.lys-immune spleen cells in vitro. if the suppressor thymus cells were cultured with lys in vitro, they produced soluble factor which depressed the ... | 1999 | 91573 |
| profile of orthopaedic surgical practice at a non-teaching naval regional medical center. | 1999 | 99703 | |
| gut hormones in fetal distress. | 1999 | 91067 | |
| identification of lactic acid bacteria from chili bo, a malaysian food ingredient. | ninety-two strains of lactic acid bacteria (lab) were isolated from a malaysian food ingredient, chili bo, stored for up to 25 days at 28 degreesc with no benzoic acid (product a) or with 7,000 mg of benzoic acid kg-1 (product b). the strains were divided into eight groups by traditional phenotypic tests. a total of 43 strains were selected for comparison of their sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) whole-cell protein patterns with a sds-page database of lab. iso ... | 1999 | 9925588 |
| characterization and identification of pediococcus species isolated from forage crops and their application for silage preparation. | pediococcus species isolated from forage crops were characterized, and their application to silage preparation was studied. most isolates were distributed on forage crops at low frequency. these isolates could be divided into three (a, b, and c) groups by their sugar fermentation patterns. strains la 3, la 35, and ls 5 are representative isolates from groups a, b, and c, respectively. strains la 3 and la 35 had intragroup dna homology values above 93.6%, showing that they belong to the species p ... | 1999 | 10388681 |
| the development of bactericidal yeast strains by expressing the pediococcus acidilactici pediocin gene (peda) in saccharomyces cerevisiae. | the excessive use of sulphur dioxide and other chemical preservatives in wine, beer and other fermented food and beverage products to prevent the growth of unwanted microbes holds various disadvantages for the quality of the end-products and is confronted by mounting consumer resistance. the objective of this study was to investigate the feasibility of controlling spoilage bacteria during yeast-based fermentations by engineering bactericidal strains of saccharomyces cerevisiae. to test this nove ... | 1999 | 10392443 |
| cloning and expression of the pediocin operon in streptococcus thermophilus and other lactic fermentation bacteria. | production of pediocin in pediococcus acidilactici is associated with pmbr1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. these four genes are organized as an operon under control of a single promoter. we have constructed shuttle vectors that contain all four structural genes, the chromosomal promoter st(p2201) from streptococcus thermophilus, and repa from the 2-kbp s. thermophilus plasmid per8. the recombinant plasmi ... | 1999 | 10489440 |
| reduction of escherichia coli o157:h7 by stimulated pediococcus acidilactici. | this study was conducted to determine if stimulating the growth of meat starter culture (pediococcus acidilactici) in a laboratory medium (brain heart infusion broth +2.3% nacl + 1.5% sucrose; lbhi) and during meat fermentation would control escherichia coli o157:h7. in lbhi medium without p. acidilactici, the numbers of e. coli o157:h7 increased from 4.00 to 8.34 log10 cfu ml-1, whereas in the presence of p. acidilactici (approximately 6.0 log10 cfu ml-1) in lbhi, lbhim (lbhi + 0.005% mnso4), l ... | 1999 | 10530043 |
| effect of diacetyl on controlling escherichia coli o157:h7 and salmonella typhimurium in the presence of starter culture in a laboratory medium and during meat fermentation. | diacetyl is a flavor compound that possesses antimicrobial activity and is found in several dairy products. the effect of diacetyl on controlling the growth of two foodborne pathogens, escherichia coli o157:h7 and salmonella typhimurium, when grown with pediococcus acidilactici as a meat starter culture was evaluated in a laboratory medium and during salami fermentation. diacetyl (50 ppm) added to each mixed culture system strongly inhibited the growth of e. coli o157:h7 and salmonella typhimuri ... | 1999 | 10492469 |
| classification and characterization of lactic acid bacteria isolated from the intestines of common carp and freshwater prawns. | fifty-four isolates of lactic acid bacteria were obtained from the intestines of the common carp (cyprinus carpio) and freshwater prawns (macrobrachium rosenbergii) in nakorn-pathom province, thailand. all isolates were gram-positive and catalase-negative cocci that did not produce gas from glucose and formed dl or l(+) lactic acid only. most isolates were able to grow in broth at ph 9.6, in 6.5% nacl (w/v) and 40% (w/v) bile. these isolates were divided into six groups (a-f) by sugar fermentati ... | 1999 | 12501375 |
| expanded bed adsorption as a unique unit operation for the isolation of bacteriocins from fermentation media. | expanded bed adsorption using a strong cation exchanger allowed the direct isolation of amylovorin l471, a bacteriocin from lactobacillus amylovorus dce 471, from the fermentation medium. the ph of the loading and elution buffer were optimised in a packed bed with cell-free culture supernatant. bound bacteriocin was eluted with 1.0 m nacl. the highest recovery (30%) was obtained at the lowest ph (3.6). at higher ph values the recovery was lower, namely 12%, 15% and 7% at ph 4.5, 6.5 and 8.0, res ... | 1999 | 10734568 |
| induction and characterization of pediococcus acidilactici temperate bacteriophage | mitomycin c was used to induce temperate bacteriophage from three strains of pediococcus acidilactici. the new bacteriophage, designated pa97, pa40, and pa42, were characterized based on morphology, dna homology, and major protein profiles. morphological attributes (small isometric heads with non-contractile tails) place these bacteriophages within the b1 group of the family siphovirdae. restriction endonuclease digests suggested that the bacteriophage genomes were linear molecules without cohes ... | 1999 | 10794138 |
| antibodies to a synthetic 1-9-n-terminal amino acid fragment of mature pediocin pa-1: sensitivity and specificity for pediocin pa-1 and cross-reactivity against class iia bacteriocins. | polyclonal antibodies specific for pediocin pa-1 (peda1) were generated by immunization of rabbits with a chemically synthesized 1-9-n-terminal amino acid fragment of this bacteriocin (ph1) conjugated to the carrier protein keyhole limpet haemocyanin (klh). the ph1 fragment holds a highly conserved amino acid sequence with closely related class iia bacteriocins. the sensitivity and specificity of the ph1-klh-generated rabbit polyclonal antibodies were evaluated by the development of various elis ... | 1999 | 10537199 |
| enhanced production of pediocin pa-1 and coproduction of nisin and pediocin pa-1 by lactococcus lactis. | the production and secretion of class ii bacteriocins share a number of features that allow the interchange of genetic determinants between certain members of this group of antimicrobial peptides. lactococcus lactis il1403 encodes translocatory functions able to recognize and mediate secretion of lactococcin a. the ability of this strain to also produce the pediococcal bacteriocin pediocin pa-1, has been demonstrated previously by the introduction of a chimeric gene, composed of sequences encodi ... | 1999 | 10508073 |
| synergistic lethal combination of nitrite and acid ph on a verotoxin-negative strain of escherichia coli o157. | this study was concerned with the possible consequences of reducing the nitrite concentration of a fermented sausage environment on the survival of the pathogen e. coli o157:h45, a verotoxin-negative relative of e. coli o157:h7. a liquid medium, fm, was constructed with a liquid phase, a(w) and ph similar to fermented sausage. survival of e. coli o157:h45 in fm depended on both ph and nitrite concentration. in trials in which the ph was decreased by growing pediococcus acidilactici in fm, surviv ... | 2000 | 10791753 |
| genomic subpopulations within the species pediococcus acidilactici detected by multilocus typing analysis: relationships between pediocin ach/pa-1 producing and non-producing strains. | a high degree of genetic polymorphism among p. acidilactici strains was highlighted by a multilocus typing approach analysing several housekeeping genes and by sampling the whole genome using random amplified polymorphic dna (rapd) fingerprint analysis performed by using a single primer peda gene targeted in low-stringency amplification conditions. restriction fragment length polymorphism of the rpoc, ldhd/l and mle genes, and a modified rapd analysis, permitted the grouping of pediococcus acidi ... | 2000 | 10931907 |
| solvent extraction of bacteriocins from liquid cultures. | a solvent extraction method was developed to concentrate lacidin from the culture of lactobacillus acidophilus osu133. the new method concentrates the bacteriocin at the interface between chloroform and the aqueous culture of the producing bacterium. compared with other extraction procedures, the new method effectively recovers higher bacteriocin yield and results in relatively clean preparations. recovery of lacidin by the chloroform extraction procedure, compared with ammonium sulphate precipi ... | 2000 | 10972727 |
| biochemical and genetic characterization of coagulin, a new antilisterial bacteriocin in the pediocin family of bacteriocins, produced by bacillus coagulans i(4). | a plasmid-linked antimicrobial peptide, named coagulin, produced by bacillus coagulans i(4) has recently been reported (b. hyronimus, c. le marrec and m. c. urdaci, j. appl. microbiol. 85:42-50, 1998). in the present study, the complete, unambiguous primary amino acid sequence of the peptide was obtained by a combination of both n-terminal sequencing of purified peptide and the complete sequence deduced from the structural gene harbored by plasmid i(4). data revealed that this peptide of 44 resi ... | 2000 | 11097892 |
| development of molecular rapd marker for the identification of pediococcus acidilactici strains. | a rapd analysis performed using a single primer targeted to the pediocin ach/pa-1 gene was carried out on several p. acidilactici strains and on some related species of lactic acid bacteria. the high degree of genetic variability detected in p. acidilactici strains did not allow the selection of a common rapd fragment that could be chosen as a potential species-specific dna marker. nevertheless a 700 bp fragment, that was found to be peculiar of all potential pediocin producer strains analyzed, ... | 2000 | 11108020 |
| cloning and expression of a plasmid-linked pediocin determinant trait of pediococcus acidilactici f. | plasmid dna from pediococcus acidilactici f was prepared by lysozyme-mutanolysin method and purified by cesium chloride-ethidium bromide (cscl-etbr) density gradient ultracentrifugation. agarose gel electrophoresis of plasmid dna and plasmid-curing experiments suggested that bacteriocin activity was harboured on a small plasmid of approximately 9.1 kb (kilobasepair) in pediococcus acidilactici f. plasmid encoding bacteriocin production in p. acidilactici f was examined for restriction enzyme cle ... | 2000 | 10746198 |
| characterization and determination of origin of lactic acid bacteria from a sorghum-based fermented weaning food by analysis of soluble proteins and amplified fragment length polymorphism fingerprinting. | the group that includes the lactic acid bacteria is one of the most diverse groups of bacteria known, and these organisms have been characterized extensively by using different techniques. in this study, 180 lactic acid bacterial strains isolated from sorghum powder (44 strains) and from corresponding fermented (93 strains) and cooked fermented (43 strains) porridge samples that were prepared in 15 households were characterized by using biochemical and physiological methods, as well as by analyz ... | 2000 | 10698775 |
| stimulation of starter culture for further reduction of foodborne pathogens during salami fermentation. | this study was conducted to determine if stimulated meat starter culture (msc; pediococcus acidilactici) would further control escherichia coli o157:h7, listeria monocytogenes, and staphylococcus aureus during salami fermentation. manganese ion (0.005% of mnso4) was used as a stimulator for the growth and acid production of msc. after 24-h salami fermentation, nonstimulated msc and stimulated msc reduced e. coli o157:h7 levels by 1.3 and 2.3 log10 units, respectively. nonstimulated msc reduced l ... | 2000 | 11079689 |
| purification, characterization, and primary structure of a novel cell wall hydrolytic amidase, cwha, from achromobacter lyticus. | a novel bacteriolytic enzyme cwha (cell wall hydrolytic amidase) was purified by ion exchange and gel-filtration chromatographies from a commercial bacteriolytic preparation from achromobacter lyticus. cwha exhibited optimal ph at 8.5 and lysed chcl(3)-treated escherichia coli more efficiently than micrococcus luteus, staphylococcus aureus, enterococcus faecalis, and pediococcus acidilactici. the enzyme was inhibited by 1,10-phenanthroline strongly and by edta to a lesser extent, suggesting that ... | 2000 | 10833271 |
| a case of septicemia with pediococcus acidilactici after long-term antibiotic treatment. | presented here is a case of septicemia caused by an uncommon, multiresistant, gram-positive microorganism (pediococcus acidilactici) after long-term antibiotic treatment. pediococcus spp. are rarely cultivated from clinical specimens, and species differentiation is difficult due to the paucity of phenotypic traits. in this case, a polyphasic approach consisting of phenotypic and molecular genetic analyses was used, and the identification of pediococcus acidilactici was conclusive. precise identi ... | 2000 | 11205633 |
| screening of commercial meat starter cultures at low ph and in the presence of bile salts for potential probiotic use. | the survival of lactic acid bacterial strains from eight meat starter cultures under conditions similar to those in the gastrointestinal tract was determined. the conditions in stomach were simulated by using ph 1-5 phosphate-buffered saline. the conditions in small intestine were simulated by using mrs broth over a ph range 4-7 and two bile salt concentrations (0.15% and 0.30%). the survival capacity at ph 3 was strain dependent. 0.30% bile salts were critical for screening bile salt tolerant s ... | 2000 | 22061286 |
| [improvement of blood viscosity after infusion of low-molecular hydroxyethyl starch (expafusin) in healthy subjects]. | in healthy volunteers, 500 ml of a 6% low molecular weight hydroxyethyl starch solution (expafusin) was infused intravenously within 30 minutes. blood samples for the measurement of rheological parameters were obtained before and after the infusion. blood and plasma viscosity were significantly decreased after the infusion of hes. red cell deformability as measured by a filtration technique was slightly improved. red cell aggregation as measured by a light transmission method was markedly reduce ... | 2000 | 94316 |
| [diminished density area surrounding the meningioma--comparison with angiographic findings-- (author's transl)]. | 2000 | 94658 | |
| detection of b cell antigens in multiple sclerosis. use of serum from multiparous women. | sera from two multiparous wives of patients with multiple sclerosis (ms) were used to detect b cell antigens in other patients. with serum x, 11 of 16 patients were positive as compared with ten of 16 controls (.05 less than p less than .1). with serum y, a positive response was found in 11 of 16 patients and two of 23 controls (p less than .0005). ten of the 11 patients who were positive with serum y were also hla-dw2, which suggests that the b cell antigen detected by this serum is linked to d ... | 2000 | 92303 |
| prostacyclin overproduction in bartter's syndrome. | 2000 | 91898 | |
| specificity of micro-leucocyte adherence inhibition test in patients with gastric cancer. | specificity of micro-leucocyte adherence inhibition (lai) test was studied in patients with gastric cancer. because of the presence of nonspecific reactions, specificity appeared obscure unless interaction analysis was applied to the results of lai tests. application of interaction analysis revealed that only leucocytes from patients with gastric cancer showed specificity to gastric cancer antigen. further technical improvement is required to eliminate nonspecific reactions which seem to be due ... | 2000 | 92433 |
| a surgical treatment of bat ears. | 2000 | 92130 | |
| plasma cells and immunoglobulin-synthesis in oral precancer and cancer. correlation with dysplasia, cancer differentiation, radio- and chemotherapy. | the subepithelial and peritumoral inflammatory infiltrates of 202 oral premalignant and malignant lesions -- 108 leukoplakias and 94 squamous cell carcinomata with different grades of dysplasia were examined using an immunoenzymatic method. in addition, the influence of radiation and bleomycin-therapy on the stromal reaction of 24 carcinomata was studied. the incidence of immunoglobulin labelled plasma cells (iga and igg) was twice as high in those cases of leukoplakia where dysplasia was presen ... | 2000 | 92096 |
| failure of lymphocyte-membrane hla-a and -b expression in two siblings with combined immunodeficiency. | 2000 | 92382 | |
| screening for congenital hypothyroidism: results of screening one million north american infants. | pilot programs for screening of newborn infants for congenital hypothyroidism began in north america in 1972. to date, the five oldest programs (quebec, pittsburgh, toronto, oregon regional, and new england regional) have screened 1,046,362 infants. a total of 277 infants with congenital hypothyroidism have been detected and seven have been missed, resulting in a total of 284 affected infants in the screened population and an overall incidence of one in 3,684 live births. of the affected infants ... | 2000 | 87512 |
| electron cytochemical stains based on metal chelation. | 2000 | 92462 | |
| [hypotension controlled with isoflurane in childhood. plethysmographic aspects]. | 2000 | 3438010 | |
| presence of a hepatoma-liver antigen in the sera of patients with primary hepatocellular carcinoma. | 2000 | 92056 | |
| training of doctors in developing countries. | it is the opinion of some that health care should be delivered by "first rate doctors" with traditional training, but this is not always possible in developing areas. groups of up to 25 students at a time have learned to apply their bedside training under conditions similar to those of the rural majority in west africa with limited supervision. bedside teaching can teach fundamental skills: listening to the patients' complaints, correctly noting their signs, and evaluating their response to trea ... | 2000 | 91869 |
| [palliative radiotherapy in esophageal carcinoma]. | 2001 | 92786 | |
| seat-belts. | 2001 | 87918 | |
| [early awelysin therapy in massive pulmonary embolism after hip alloarthroplasty]. | the fibrinolytic therapy of massive frest pneumatic embolism represents an ensured progress today. until now it was only possible to report on those cases where the patients had survived at least two hours from the beginning of the acute event. two cases are reported here where the patients were successfully treated in spite of a standstill in the circulation. in a 60 years old female patient and 54 years old female hypertonic patient a successful reanimation could be performed in the early post ... | 2001 | 94891 |
| chronic progressive myelopathy: investigation with csf electrophoresis, evoked potentials, and ct scan. | chronic progressive myelopathy (cpm) is a difficult clinical problem. many patients who present with cpm turn out to have a spinal form of multiple sclerosis (ms), but until there is clear lesion dissemination, a definite clinical diagnosis cannot be made. we have looked for ms-related abnormalities in 72 patients with cpm. the mean age of onset was 42 years, mean duration was ten years, and mean kurtzke disability rating was 4.5. studies performed were cerebrospinal fluid electrophoresis for ol ... | 2001 | 92910 |
| [comparative study of the average longevity of males and females in non-soviet countries]. | 2001 | 90391 | |
| [occurrence and distribution of multiple forms of formamidase in various yeasts]. | 2001 | 92861 | |
| optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate pcr. | to reduce pcr bias derived from a primer mismatch, the effect of the annealing temperature on the product ratio was investigated by denaturing gradient gel electrophoresis analysis of pcr products from a mixture of perfect-match and one-mismatch templates. these templates were generated by pcr from pediococcus acidilactici for one mismatch and micrococcus luteus for the perfect match. pcrs showed that the bias was reduced at lower temperatures. an environmental sample was also examined. | 2001 | 11472961 |
| characterisation of is153, an is3-family insertion sequence isolated from lactobacillus sanfranciscensis and its use for strain differentiation. | an insertion sequence has been identified in the genome of lactobacillus sanfranciscensis dsm 20451t as segment of 1351 nucleotides containing 37-bp imperfect terminal inverted repeats. the sequence of this element encodes two out of phase, overlapping open reading frames, orfa and orfb, from which three putative proteins are produced. orfab is a transframe protein produced by -1 translational frame shifting between orf a and orf b that is presumed to be the transposase. the large orfab of this ... | 2001 | 11822682 |
| phenotypic and genotypic characterization of pediococcus strains isolated from human clinical sources. | seventy-two strains of pediococci isolated from human clinical sources were characterized by conventional physiological tests, chromogenic enzymatic tests, analysis of whole-cell protein profiles (wcpp) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and analysis of chromosomal dna restriction profiles by pulsed-field gel electrophoresis (pfge). conventional tests allowed identification of 67 isolates: 52 strains were identified as pediococcus acidilactici, 15 strains were identifi ... | 2001 | 11283035 |
| effect of physical factors on the production of bacteriocin from pediococcus acidilactici itv 26. | the effect of ph, temperature and agitation on growth and bacteriocin production by pediococcus acidilactici itv 126 was investigated. experiments were made in flasks containing mrs medium at 30 to 40 degrees c, ph 5 to 7 and agitation 0 to 200 rpm. factor levels were arranged in a 2(3) factorial design with central and axial points. anova and tukey paired comparison tests showed that a temperature of 35 degrees c favored bacteriocin production, whereas 40 degrees c was best for cell growth. a s ... | 2001 | 11464266 |
| nutritional factors affecting the production of two bacteriocins from lactic acid bacteria on whey. | the ability of lactococcus lactis subsp. lactis cect 539 and pediococcus acidilactici nrrl b-5627 to produce bacteriocins on both diluted and concentrated whey was investigated in batch fermentations. both strains produced the higher amounts of biomass and bacteriocin titres on diluted whey. luedeking and piret expression was able to model the production of nisin, which was produced as a primary metabolite on both culture media. however, the pediocin production could not be typified in any case ... | 2001 | 11764192 |
| selection and characterization of mixed starter cultures for lactic acid fermentation of carrot, cabbage, beet and onion vegetable mixtures. | an evaluation of various lactic acid bacteria (lab) for the fermentation of cabbage, carrot and beet-based vegetable products was carried out. as part of a screening process, the growth of 15 cultures in a vegetable juice medium (vjm) was characterized by automated spectrophotometry. acidification patterns as well as viability during storage of the lab were also established. there were greater differences between the pure cultures than the mixed ones with respect to growth in vjm and viability d ... | 2001 | 11294348 |
| novel food-grade plasmid vector based on melibiose fermentation for the genetic engineering of lactococcus lactis. | the alpha-galactosidase gene (aga) and a gene coding for a putative transcriptional regulator from the laci/galr family (galr) of lactococcus raffinolactis atcc 43920 were cloned and sequenced. when transferred into lactococcus lactis and pediococcus acidilactici strains, aga modified the sugar fermentation profile of the strains from melibiose negative (mel(-)) to melibiose positive (mel(+)). analysis of gala mutants of l. lactis subsp. cremoris mg1363 indicated that the putative galactose perm ... | 2002 | 12450840 |