Publications

the importance of timing for the action of alpha 1- or beta-agonists: possible relationship with the density of [3h]prazosin and [3h]dihydroalprenolol binding sites.	exploratory behaviour was measured in the open-field at 8.00 h and 20.00 h with naive rats and rats treated with an alpha 1-agonist (phenylephrine, 25 micrograms i.c.v.) or beta-agonists (salbutamol, 2.5 mg/kg; clenbuterol 0.05 mg/kg i.p.). the spontaneous activity was similar at both times, yet the response to a alpha 1-as well as to beta-stimulation was significantly enhanced at 20.00 h. the density of cortical [3h]prazosin and [3h]dihydroalprenolol ([3h]dha) binding sites at 20.00 h was enhan ...	1985	2864268
an aids patient who died too soon.		1985	3917452
an aids patient who died too soon.		1985	3917452
mechanism of cyclosporin a-induced inhibition of prostacyclin synthesis by macrophages.	in vitro studies of pg production over a 24 h period by adherent rat peritoneal macrophages activated by serum-opsonized zymosan revealed that csa (0.3-10 micrograms/ml) caused a dose-related inhibition of pgi2 (assayed as 6-oxo-pgf1 alpha) formation. indomethacin (ind, 0.01-10 micrograms/ml) and dexamethasone (dex, 0.01-10 micrograms/ml) also inhibited the pg production in a dose-related manner. when arachidonic acid (10 micrograms/ml) was added together with the inhibitors, there was no change ...	1985	3936119
[review on internal medicine, 1984. electrolyte disturbance. ii-1. potassium homeostasis and its abnormality].		1985	4045285
mechanism of cyclosporin a-induced inhibition of prostacyclin synthesis by macrophages.	in vitro studies of pg production over a 24 h period by adherent rat peritoneal macrophages activated by serum-opsonized zymosan revealed that csa (0.3-10 micrograms/ml) caused a dose-related inhibition of pgi2 (assayed as 6-oxo-pgf1 alpha) formation. indomethacin (ind, 0.01-10 micrograms/ml) and dexamethasone (dex, 0.01-10 micrograms/ml) also inhibited the pg production in a dose-related manner. when arachidonic acid (10 micrograms/ml) was added together with the inhibitors, there was no change ...	1985	3936119
quantitative and temporal analyses of murine antibody response in serum and gut secretions to infection with giardia muris.	we analyzed the appearance and level of giardia muris-specific antibody of immunoglobulin a (iga), igg, and igm isotypes, at weekly intervals, over the course of a 7-week infection in balb/c and c57bl/6 mice. using sensitive immunoradiometric assays, we observed that iga antibody was the only detectable anti-g. muris antibody in intestinal secretions throughout the course of infection. no secreted igg or igm anti-g. muris antibody was detected even in concentrated intestinal secretions. the expu ...	1986	3957428
quantitative and temporal analyses of murine antibody response in serum and gut secretions to infection with giardia muris.	we analyzed the appearance and level of giardia muris-specific antibody of immunoglobulin a (iga), igg, and igm isotypes, at weekly intervals, over the course of a 7-week infection in balb/c and c57bl/6 mice. using sensitive immunoradiometric assays, we observed that iga antibody was the only detectable anti-g. muris antibody in intestinal secretions throughout the course of infection. no secreted igg or igm anti-g. muris antibody was detected even in concentrated intestinal secretions. the expu ...	1986	3957428
stereochemistry of estrogen biosynthesis by a reconstituted aromatase cytochrome p-450 preparation from human placenta.	according to the literature, the multistep reaction mechanism of estrogen biosynthesis proceeds with stereospecific loss of the equatorial 1 beta-, and axial 2 beta-protons. these results were deduced from experiments carried out, either with crude microsomes, or at best with impure enzyme extracts. however, when [1,2- 3h]4-androstene-3,17-dione of known absolute 3h-label distribution was incubated with a reconstituted enzyme system, consisting of homogeneous nadph-cytochrome p-450 reductase and ...	1986	3707583
comparative effects of caerulein on food intake and pancreatic secretion in dogs.	we compared effects of the cck analog caerulein on feeding and pancreatic secretion. nine fasted mongrel dogs with gastric and pancreatic fistulas received scalar doses of caerulein (0, 6.25, 12.5, 25, 50, 100, 200, 400 pmol/kg-hr, each for 30 min). the d50 dose for stimulation of pancreatic secretion was 15 pmol/kg-hr. effects of intravenous caerulein (0 to 800 pmol/kg-hr; 15 min before and during a 45 min test meal) on food intake were examined in 8 beagles under 4 feeding conditions: 1 meal/d ...	1986	3768748
multiple biochemical deficits in both gray and white matter of alzheimer brains.	a biochemical investigation of 21 brains from patients with dementia of alzheimer type (ad/sdat) and 22 brains from controls was made. in the normal brains 5-hydroxytryptamine (5-ht), noradrenaline (na) and dopamine (da) were reduced with age while their metabolites were not reduced. in the brains from the alzheimer patients the examination of gray matter (caudate nucleus and hippocampus) showed a significant reduced activity of choline-acetyl transferase (cat) and a reduction of acetylcholinest ...	1986	3797685
new aspects of the epidemiology of hepatitis delta virus infection?		1986	3804179
effect of methyl mercury on prostaglandin synthesis in rabbit kidney medulla slices.	effects of methylmercuric chloride (mmc) in the absence and presence of ascorbic acid on the synthesis of prostaglandins e2 and f2 alpha, and the lipid peroxidation of rabbit kidney medulla slices were studied. mmc showed dose-dependent inhibition of prostaglandins e2 and f2 alpha formation. the lipid peroxidation was not stimulated by mmc alone. ascorbic acid plus mmc were able to stimulate the lipid peroxidation markedly, but the lipid peroxidation induced by ascorbic acid plus mmc had no infl ...	1986	3467401
control of red cell urea and water permeability by sulfhydryl reagents.	the binding constant for pcmbs (p-chloromercuribenzenesulfonate) inhibition of human red cell water transport has been determined to be 160 +/- 30 microm and that for urea transport inhibition to be 0.09 +/- 0.06 microm, indicating that there are separate sites for the two inhibition processes. the reaction kinetics show that both processes consist of a bimolecular association between pcmbs and the membrane site followed by a conformational change. both processes are very slow and the on rate co ...	1986	3017418
the role of pulmonary cellular reactions in the resistance of vaccinated mice to schistosoma mansoni.	a histopathological and ultrastructural study was made of schistosomula and associated inflammatory reactions in the lungs of normal mice, and mice previously vaccinated with irradiated cercariae. in normal mice at day 7 post-infection all schistosomula were located in blood vessels. from day 11 onwards an increasing proportion of schistosomula were intra-alveolar (80% from day 20). no cellular reactions were evident around intravascular parasites in normal mice but at later sampling times large ...	1986	3088537
[experimental and clinical studies of sutures of natural silk produced by oak silkworms].		1986	3716085
regulation of enzymes by ligand induced change in polymerization.		1986	3020906
in-vitro study of the enkephalinergic hypothesis for non-adrenergic, non-cholinergic innervation in the cat stomach.	it has been suggested that enkephalins are involved in the gastric relaxation induced by stimulation of the non-adrenergic, non-cholinergic vagal fibres in the cat stomach. experiments were therefore performed on strips of cat stomach. with longitudinal and circular gastric fundus and corpus strips from reserpinized cats, non-adrenergic, non-cholinergic relaxatory responses could be elicited by transmural electrical stimulation in tyrode solution containing atropine and 5-hydroxytryptamine. morp ...	1986	2869124
the inhibition by valproic acid of the mitochondrial oxidation of monocarboxylic and omega-hydroxymonocarboxylic acids: possible implications for the metabolism of gamma-aminobutyric acid.	the interactions of 1-5 mm valproic acid with the hepatic fatty acid oxidation are here described. valproic acid was not substrate for hepatic peroxisomal fatty acid oxidation. its activation outside the mitochondrial matrix compartment was poor when compared to that of octanoic acid, a fatty acid containing the same number of carbones. valproic acid did not inhibit the fatty acyl-coa oxidase nor the cyanide-insensitive acyl-coa oxidation. valproic acid inhibited the mitochondrial oxidations of ...	1987	3117781
[clinical application of energy subtraction imaging using digital radiography for excretory urography in infants].		1987	3329251
rna fingerprinting using a small horizontal agarose gel electrophoresis apparatus.	the electrophoresis step in the rna fingerprinting procedure has been modified by replacing the large and expensive electrophoresis tank with a horizontal agarose gel electrophoresis box, a common piece of laboratory equipment. two rna molecules (67 and 298 nucleotides long) transcribed in vitro from a truncated human beta-globin gene have been fingerprinted and analyzed by the modified technique. the resolution of the oligonucleotides in this procedure is comparable to that seen using the tank ...	1987	2440342
[proliferative activity of experimental brain tumors].	the method of autoradiography was used to study the parameters of the cell cycle and the intensity of proliferative processes in three transplantable rat brain tumors differing in the duration of the latent period and sensitivity to chemotherapy. the tumors were found to be similar in the duration of the cell cycle and its phases, the mean values of the label index and growth fraction, and were characterized by the irregularity of the proliferative processes within the tumor; the label index was ...	1987	3564792
[primary leiomyosarcoma of the pulmonary artery. apropos of a case].	a case of leiomyosarcoma of the pulmonary artery in a 64-year old man without previous cardiovascular disease is reported. the clinical picture, which comprised episodes of paroxysmal dyspnoea associated with acute cor pulmonale, suggested pulmonary embolism. radioisotope perfusion study and pulmonary angiography seemed to confirm this diagnosis, but no improvement was obtained with a prolonged thrombolytic treatment. the presence of a median mass at ct led to exploratory thoracotomy and to the ...	1987	3122693
[extraintestinal isolation of salmonella in chickens: epidemiologic study of 3 salmonellosis outbreaks after ingestion of raw eggs].	a taxonomic analysis of the malolactic microflora present in nine grape samples from different zones of galicia, was carried out. nineteen strains were isolated and identified as lactobacillus plantarum (42%), l. brevis (10.6%), l. casei (5.25%), l. hilgardii (5.25%), streptococcus cremoris (5.25%), leuconostoc lactis (5.25%), l. oenos (10.6%), and pediococcus acidilactici (15.8%).	1987	3077329
[taxonomic study of epiphyte lactic bacteria in grapes in galicia].	a taxonomic analysis of the malolactic microflora present in nine grape samples from different zones of galicia, was carried out. nineteen strains were isolated and identified as lactobacillus plantarum (42%), l. brevis (10.6%), l. casei (5.25%), l. hilgardii (5.25%), streptococcus cremoris (5.25%), leuconostoc lactis (5.25%), l. oenos (10.6%), and pediococcus acidilactici (15.8%).	1987	3268295
drug permeability of the endolymphatic sac.	intravenously injected kanamycin sulfate (km) was located in the endolymphatic sac (es) of guinea pigs by an immunohistologic method. kanamycin sulfate had passed through the capillaries in the subepithelial layer and reached the epithelial layer as early as 1 minute after injection. the amount of km in the epithelial layer gradually increased with time (until 30 minutes after injection). the km also accumulated in floating cells in the es. these results tend to indicate that the es is rather ea ...	1987	3304088
antimicrobial activity of ro 19-5247 (t-2525), a new oral cephalosporin, tested against 7,745 recent clinical isolates.	the susceptibility testing of 7,745 recent clinical isolates from four medical centers showed ro 19-5247 to be eight- to greater than 64-fold more active than cephalexin against the enterobacteriaceae. ro 19-5247 was comparable with cephalexin in anti-staphylococcal activity (mic50, 4.0 micrograms/ml) and fourfold more active than cefixime. none of the oral cephalosporins were effective (mic50, greater than 32 micrograms/ml) against enterococci, pseudomonas aeruginosa and p. maltophilia. beta-la ...	1987	3568594
pathogenesis of trachoma: the stimulus for inflammation.	active trachoma is characterized by chronic inflammation of the conjunctiva, and repeated episodes of reinfection are thought to be necessary to sustain this inflammation. it is currently believed that much of the tissue damage is immunologically mediated. to identify which antigens might be responsible for stimulating this continued inflammation, cynomolgus monkeys that had recovered from a previous ocular infection with chlamydia trachomatis were challenged with various antigen preparations. p ...	1987	3571982
csf/shunt infections--the bane of our lives!	thirty one patients were treated for 43 laboratory proven shunt or csf infections, between 1980 to 1986 inclusive. they required 63 separate treatments. infective episodes were grouped retrospectively according to method of treatment employed i.e. 1) placement of external drainage system or 2) reservoir and tapping, 3) intravenous or intraventricular antibiotics alone, 4) total change of shunt prosthesis, 5) partial change of prosthesis or 6) removal of all the prosthesis. see table ii. the sing ...	1987	3433967
cardiovascular and metabolic response of tumour-bearing dogs to whole body hyperthermia.	whole body hyperthermia was induced in older, tumour-bearing dogs using a radiant heat device. dogs were anaesthetized (thiopental), paralysed (atracurium), and mechanically ventilated (100 per cent o2) during the heating procedure. heart rate, systolic/diastolic, and mean arterial blood pressure, and arterial blood gases were monitored throughout the procedure. serum biochemical parameters, complete blood count, and coagulation profiles were evaluated before, during, and after whole body hypert ...	1987	3693985
effect of hyponatremia on response to volume depletion in uremic dogs.	it has been suggested that sodium depletion can alter cardiovascular reflexes and vascular sensitivity to pressors. to define the effects of hyponatremia on the hemodynamic response to volume depletion in the setting of uremia, isolated ultrafiltration was performed in acutely uremic dogs previously rendered hyponatremic (127 + 2.5 [sd] meq/l) for 72 hours by dialyzing against a low-sodium dialysate. response was compared with that of the same animals in the normonatremic (150 +/- 3.6 meq/l) con ...	1987	3794518
nacl and ca delivery at the bend of rat deep nephrons decreases during antidiuresis.	the effects of 1-desamino-8-d-arginine vasopressin (ddavp) on superficial and juxtamedullary nephrons were investigated by micropuncture in diabetes insipidus (di) brattleboro rats chronically treated with the peptide. the rats, acutely deprived of endogenous calcitonin, parathyroid hormone (pth), and glucagon [hormone-deprived (hd) rats], were examined either 4 days after cessation of ddavp treatment (hdt, control diuretic rats) or when the treatment was continued until the micropuncture experi ...	1987	3591953
protected methionine supplementation with extruded blend of soybeans and soybean meal for dairy cows.	methionine may be the first amino acid limiting milk production in early lactation cows. to evaluate this further, 23 high producing holstein cows (9 multiparous and 14 primiparous) were fed an extruded blend of soybeans and soybean meal (40:60) without or with 15 g of added dl-methionine as 50 g of ruminally protected methionine product during wk 4 to 16 postpartum. cows were fed a 15.8% crude protein total mixed ration consisting of 30% (dry basis) corn silage, 15% alfalfa hay, and 55% concent ...	1987	3571638
plasmid-associated bacteriocin production and sucrose fermentation in pediococcus acidilactici.	production of bacteriocin activity designated pediocin pa-1 was associated with the presence of a 6.2-megadalton plasmid in pediococcus acidilactici pac1.0. the bacteriocin exhibited activity against p. acidilactici, p. pentosaceus, lactobacillus plantarum, l. casei, l. bifermentans, and leuconostoc mesenteroides subsp. dextranicum. partial characterization of pediocin pa-1 is described. the molecular weight of pediocin pa-1 was ca. 16,500. additionally, strain pac1.0 was found to contain a 23-m ...	1987	16347470
studies on competitive saprophytic colonization of sclerotium rolfsii sacc. causing foot-rot disease of barley under certain pesticidal treatments.		1988	3255750
effects of sulfonylureas on the synthesis and secretion of plasminogen activator from bovine aortic endothelial cells.	the effects of sulfonylureas on the production of plasminogen activator (pa) and antiactivator (pai) were investigated using bovine aortic endothelial cells. all compounds studied stimulated pa release (1.3- to 5.2-fold), with glipizide being the most potent, followed by tolazamide, chlorpropamide, and tolbutamide, in that order, while glyburide was the least effective. both tissue-type and urokinase-type pa production was enhanced. studies using metabolic inhibitors indicated that both rna and ...	1988	3125227
histochemistry of cerebral lesions in mice infected with plasmodium berghei.	the brains of mice with established symptoms of plasmodium berghei erebral malaria were investigated histochemically and histologically. the activity of mitochondrial, lysosomal, glyco/glycogenolytic, hydrolytic and oxidizing enzymes as well as enzymes of the krebs cycle and the pentose cycle was studied during the course of the infection. for comparison cryostate sections were also stained with haematoxylin and eosin, and according to kluver-barrera. changes in enzyme activity, particularly of ...	1988	3067319
postural adjustments in the monkey: effects of velocity on emg sequence.	monkeys were trained to perform flexion or extension movements of the elbow in a strictly restrained position. even in these conditions, postural adjustments were still recorded. they appeared to be organized in a consistent pattern. when trials were ranked with increasing velocity, two kinds of emg changes could be described: (i) those in the upper part of the body which may be triggered by anticipatory postural motor programs; (ii) those in lower limbs recruited only with higher velocities and ...	1988	3126464
follicle-stimulating hormone induces deoxyribonucleic acid polymerase alpha before deoxyribonucleic acid synthesis in immature rat ovarian follicles perfused in vitro.	to examine the effects of follicle-stimulating hormone (fsh) on cell proliferation and cell division in ovaries, deoxyribonucleic acid (dna) polymerase activities and dna concentrations were determined in ovarian follicles of immature, hypophysectomized, estradiol-treated rats perfused in vitro. ovine fsh (2-20 micrograms/ml) significantly enhanced the dna polymerase alpha activity in the follicles perfused for 180-300 minutes, whereas the dna polymerase beta activity and dna concentration showe ...	1988	3126468
inhibition of listeria monocytogenes by using bacteriocin pa-1 produced by pediococcus acidilactici pac 1.0.	the bacteriocin produced by pediococcus acidilactici pac 1.0, previously designated pa-1 bacteriocin, was found to be inhibitory and bactericidal for listeria monocytogenes. a dried powder prepared from pac 1.0 culture supernatant fortified with 10% milk powder was found to contain bacteriocin activity. an mic against l. monocytogenes and lytic effects in broth cultures were determined. inhibition by pa-1 powder occurred over the ph range 5.5 to 7.0 and at both 4 and 32 degrees c. in addition, i ...	1988	3144243
lack of an effect of 6-hydroxydopamine lesions of the nucleus accumbens on intravenous morphine self-administration.	the neurotoxin, 6-hydroxydopamine (6-ohda), has been used to selectively destroy dopamine containing neurons in discrete brain regions. lesions of the nucleus accumbens with this neurotoxin decrease or eliminate cocaine and amphetamine self-administration and either increase or do not affect opiate self-administration in rats with unrestricted access to food and water. this study reports the effects of 6-ohda lesions of the nucleus accumbens on responding maintained by food, water or morphine (3 ...	1988	3147458
radiation sensitivity of human ovarian carcinoma cell lines in vitro: effects of growth factors and hormones, basement membrane, and intercellular contact.	the radiation sensitivity of six established human ovarian carcinoma cell lines was determined in vitro under five different experimental conditions. cells from exponentially growing monolayer cultures were assayed under three different post-irradiation culture conditions, i.e., conventional conditions on a plastic surface, in the presence of growth factors and hormones, and on a basement membrane. multicellular aggregates were dissociated either immediately before or immediately after irradiati ...	1988	3182331
fine structure of the a-band in cryo-sections. diversity of m-band structure in chicken breast muscle.	electron micrographs of longitudinal ultrathin cryo-sections and plastic sections of chicken pectoralis muscle together with their average images have been used to study in detail the axial structure of the m-band. it was found that m-band structure could vary markedly in different fibres, even within the white part of the muscle. strong m-band density ("m-bridges") could be seen at m4 and m4' in all fibres. on the other hand the density at m1 or m6 could vary systematically. some fibres (probab ...	1988	3209858
purification, characterization and antimicrobial spectrum of a bacteriocin produced by pediococcus acidilactici.	an antimicrobial peptide designated pediocin ach was isolated from pediococcus acidilactici strain h. the pediocin ach was purified by ion exchange chromatography. the molecular weight of pediocin ach was determined by sds-page to be about 2700 daltons. pediocin ach was sensitive to proteolytic enzymes resistant to heat and organic solvents, and active over a wide range of ph. pediocin ach exhibited inhibition against several food spoilage bacteria and foodborne pathogens including staphylococcu ...	1988	2906056
oviducal storage of spermatozoa in the turkey: its relevance to artificial insemination technology.	1. this paper addresses questions concerning the role of oviducal sperm selection and storage in the relative inefficiency of turkey reproduction. 2. before the onset of egg production, the turkey has the biological potential to store spermatozoa in its oviduct for periods up to 5 weeks; after the onset of production fewer spermatozoa are retained by the oviduct. 3. a better understanding of the biological mechanisms which regulate sperm selection and storage in the turkey oviduct is necessary t ...	1989	2670069
identification of gram-positive coccal and coccobacillary vancomycin-resistant bacteria.	a total of 84 of 150 vancomycin-resistant (defined as no inhibition of bacterial growth around a 30-micrograms vancomycin disk placed on 5% sheep blood-trypticase soy agar [bbl microbiology systems, cockeysville, md.]) bacteria were definitively identified by determining the phenotypic criteria. the identity of representatives was also confirmed by dna-dna hybridizations. the following strains were identified: 1 enterococcus faecium, 18 leuconostoc mesenteroides, 15 leuconostoc citreum, 9 leucon ...	1989	2723037
effects of l-carnitine, l-acetylcarnitine and gangliosides on the regeneration of the transected sciatic nerve in rats.	three pharmacological agents, l-carnitine, l-acetylcarnitine and gangliosides, were tested for their ability to enhance the regeneration of the rat sciatic nerve following transection and microsurgical repair. the drugs were administered intraperitoneally at the dose of 50 mg/kg/d for 28 and 56 d postoperatively. at the end of treatment, the motor function recovery of the peroneal component of the sciatic nerve was assessed and the regenerated nerves were analysed morphometrically on histologica ...	1989	2565553
[nuclear dna cytofluorometry of normal human laryngeal epithelia and squamous cell carcinoma].	normal human laryngeal epithelia and laryngeal squamous cell carcinoma were assayed by feulgen dna cytofluorometry using free cell nuclei isolated from carnoy-fixed, paraffin-embedded specimens. in all of the 12 normal specimens, the epithelium showed typical diploid cell clones with low proliferative activity. polyploid cells were seen in only two specimens from subjects aged 61 and 69 years respectively, and the number of polyploid cells seen in these two specimens was only two. fifteen cancer ...	1989	2593035
adverse effects of the use of unusual phenethylamine compounds sold as illicit amphetamine.	a 40-year-old man, who had regularly taken illicit amphetamine by intra-nasal inhalation for several years without ill effects, was admitted to hospital with signs of massive adrenergic overstimulation shortly after inhaling material which he had purchased in the belief that it was amphetamine. the administration of the beta-blocker practolol produced a paradoxical increase in blood pressure. after his discharge from hospital he suffered disabling feelings of anxiety for several weeks. analysis ...	1989	2770471
sympathetic uveitis after giant tear repair.	a 33-year-old hispanic man developed sympathetic ophthalmia 4.5 months after vitrectomy and repeat scleral buckling for a giant retinal tear in an aphakic previously traumatized eye. the patient had gray-white, subretinal nodules with characteristic changes of sympathetic uveitis detected by fluorescein angiography. the fluorescein angiographic and clinical findings revealed a dramatic response to steroid treatment: visual acuity returned to the previous 20/20 level within 5 weeks of therapy.	1989	2772419
clinical and microbiologic characteristics of pediococci.	over a 43-month period, 23 separate isolates of nonenterococcal alpha- and nonhemolytic streptococci were reported by our clinical microbiology laboratory to be resistant to vancomycin. this constituted 0.32% of nonenterococcal alpha- and nonhemolytic streptococci reported and 4.4% of such streptococci upon which susceptibility testing was performed. of 13 isolates which were available for further study, all were highly resistant to vancomycin (mic greater than or equal to 1,024 micrograms/ml), ...	1990	2380362
vancomycin-resistant pediococcus acidilactici: nine cases of bacteremia.	pediococci, vancomycin-resistant gram-positive cocci, have been isolated from human specimens, but an association with clinical illness has not been established. clinical and epidemiologic data were obtained on nine patients who had pediococcus acidilactici isolated from blood. patients were eight elderly adults with complicated medical problems and one infant with congenital jejunoileal atresia. seven patients were hospitalized before p. acidilactici was isolated. eight had received multiple an ...	1990	2324545
antigenic property of pediocin ach produced by pediococcus acidilactici h.	pediocin ach, a bacteriocin of pediococcus acidilactici h, inhibits the growth of several food spoilage and pathogenic bacteria. the antigenic property of partially purified pediocin ach was tested by immunizing mice and a rabbit. pediocin ach was not immunogenic in these animals as determined by immunoblotting even after conjugation to bovine serum albumin. the non-immunogenic nature of pediocin ach, its non-toxicity to laboratory animals and its hydrolysis by gastric proteolytic enzymes may be ...	1990	2272942
an osmium-ferricyanide staining method for the intercellular space in the small intestinal epithelium.	the post-fixation with osmium tetroxide and potassium ferricyanide (osfecn) produced dense deposits on the outer surface of the lateral plasma membrane in the mouse small intestinal epithelium. the deposits also filled up the intercellular space. no deposits were found on the surface of apical and basal plasma membranes. this staining pattern was highly reproducible when ph of the osfecn solution was adjusted to 7.0 by cacodylate buffer without calcium ion. thus, our modified osfecn method is us ...	1990	1694215
mapping the effects of motor cortex stimulation on somatosensory relay neurons in the rat thalamus: direct responses and afferent modulation.	single unit recordings were used to map the spatial distribution of motor (mi) cortical influences on thalamic somatosensory relay nuclei in the rat. a total of 215 microelectrode penetrations were made to record single neurons in tracks through the medial and lateral ventroposterior (vpm and vpl), ventrolateral (vl), reticular (nrt), and posterior (po) thalamic nuclei. single units were classified according to their: 1) location within the nuclei, 2) receptive fields, and 3) response to standar ...	1990	2322860
native valve endocarditis due to corynebacterium group jk.	we report a case of a 32-yr-old woman on chronic intermittent haemodialysis, who developed endocarditis due to a corynebacterium group jk, involving both the native aortic and mitral valves. despite a four-week treatment with vancomycin, an aortic root abscess developed. the diagnosis was confirmed on autopsy.	1990	2074917
use of a bacteriocin produced by pediococcus acidilactici to inhibit listeria monocytogenes associated with fresh meat.	a bacteriocin produced by pediococcus acidilactici had an inhibitory and bactericidal effect on listeria monocytogenes associated with fresh meat. mics were significantly lower than minimum killing concentrations. when meat was inoculated with l. monocytogenes, the bacteriocin reduced the number of attached bacteria in 2 min by 0.5 to 2.2 log cycles depending upon bacteriocin concentration. meat treated initially with the bacteriocin resulted in attachment of 1.0 to 2.5 log cycles fewer bacteria ...	1990	2117881
septicemia caused by vancomycin-resistant pediococcus acidilactici.	a case of septicemia caused by vancomycin-resistant pediococcus acidilactici is discussed. this appears to be the first reported case of septicemia caused by this organism. the characteristics and antimicrobial susceptibilities of this organism are described.	1990	2199529
mapping the effects of motor cortex stimulation on somatosensory relay neurons in the rat thalamus: direct responses and afferent modulation.	single unit recordings were used to map the spatial distribution of motor (mi) cortical influences on thalamic somatosensory relay nuclei in the rat. a total of 215 microelectrode penetrations were made to record single neurons in tracks through the medial and lateral ventroposterior (vpm and vpl), ventrolateral (vl), reticular (nrt), and posterior (po) thalamic nuclei. single units were classified according to their: 1) location within the nuclei, 2) receptive fields, and 3) response to standar ...	1990	2322860
papillary adenocarcinoma of prostate.	papillary adenocarcinomas of the prostate are rare tumors which may arise from the prostatic ducts and the utricle; 2 cases are described. the diagnosis of these tumors is best established by cystourethroscopy and transurethral resection. the more common prostatic acinar adenocarcinoma is frequently associated with these tumors. the histopathologic recognition of papillary adenocarcinoma of the prostate and its differentiation from acinar adenocarcinoma is important since the natural history and ...	1990	184573
microbiological and biochemical changes during the fermentation of maize (zea mays) grains formasa production.	microorganisms associated with fermentation ofmasa werelactobacillus plantarum, pediococcus acidilactici, lactobacillus fermentum andsaccharomyces cerevisiae. during the fermentation of maize grains, the temperature and moisture increased and the ph decreased. most amino acids decreased in concentration during fermentation, with glutamic acid being the most abundant amino acid, but he protein content of the 'masa' increased during fermentation. the bacteria played the major role in the fermentat ...	1990	24429990
[3h]mk-801 binding to n-methyl-d-aspartate receptors solubilized from rat brain: effects of glycine site ligands, polyamines, ifenprodil, and desipramine.	the n-methyl-d-aspartate (nmda) receptor is thought to contain several distinct binding sites that can regulate channel opening. in the present experiments, the effects of ligands for these sites have been examined on [3h]mk-801 binding to a soluble receptor preparation, which had been passed down a gel filtration column to reduce the levels of endogenous small-molecular-weight substances. glycine site agonists, partial agonists, and antagonists gave effects similar to those observed in membrane ...	1991	1828832
implications of lethal anesthetic doses.		1991	1796981
implications of lethal anesthetic doses.		1991	1796981
the occurrence and growth of microorganisms during the fermentation of fish sausage.	minced fish (mullet) sausage mixes containing added sugar, salt, nitrate, nitrite and spices were fermented (48 h, 30 degrees c) by indigenous flora or by a starter culture (pediococcus acidilactici) and the microbial ecology and behaviour of various bacteria was monitored. pediococcus pentosaceus and lactobacillus plantarum dominated the indigenous fermentation, achieving populations of 10(7)-10(8) cfu/g by 48 h, and decreasing the ph of the mix to 4.5-4.7. significant growth (10(5)-10(7) cfu/g ...	1991	1909546
bacterial formation of n-nitroso compounds in the rat stomach after omeprazole-induced achlorhydria.	n-nitrosamine formation by bacteria in the achlorhydric stomach has been proposed as an important factor in the development of gastric cancer. thus, the effect of the presence of bacteria in the stomach on endogenous nitrosation was investigated in rats given omeprazole (an inhibitor of gastric h+, k((+)-atpase) which reduces gastric secretion sufficiently to allow survival of a bacterial suspension of escherichia coli or pseudomonas. when rats were given both thiazolidine 4-carboxylic acid and ...	1991	1855848
cyclophellitol: a naturally occurring mechanism-based inactivator of beta-glucosidases.	the natural product cyclophellitol, isolated from the culture filtrate of a mushroom, phellinus sp. is found to be a highly specific and effective irreversible inactivator of beta-glucosidases. it inactivates the beta-glucosidases from both almond emulsin and agrobacter sp. according to pseudo-first order kinetics with inactivation constants of ki = 0.34 mm, ki = 2.38 min-1, and ki = 0.055 mm, ki = 1.26 min-1 respectively. no reactivation of the inactivated enzyme is seen upon dialysis, thus pro ...	1991	1904221
behavior of listeria monocytogenes in wiener exudates in the presence of pediococcus acidilactici h or pediocin ach during storage at 4 or 25 degrees c.	exudative fluids were collected from packages of five brands of all-beef wieners and inoculated to contain 10(4) to 10(5) cfu of a three-strain (scott a, v7, and 101m) mixture of listeria monocytogenes per ml. listeriae were inactivated (decrease of 0.61 to 3.8 log10 cfu/ml) in all five exudates held at 4 degrees c for 29 days. l. monocytogenes grew (increase of 1.7 to 3.6 log10 cfu/ml) in two of five exudates held at 25 degrees c for 6 days. exudate was inoculated with a derivative of pediococc ...	1991	1906699
[effect of hemodynamic conditions and prosthesis structure on endothelialization of clinical and experimental small lumen vascular prostheses].	seeding of small-diameter vascular prostheses (id less than or equal to 6 mm) with autologous microvascular cells (amvc) results in a complete endothelial cell layer on the luminal surface. the purpose of this study was to examine the influence of the blood flow velocity (due to 4 or 6 mm id) and the structure of inner graft surface (crimped, uncrimped) on the endothelialization. amvc were harvested from omental adipose tissue (mean: 0.56 x 10(6) cells/g tissue) from 10 mongrel dogs (mean: 27.9 ...	1991	1828797
influence of growth conditions on the production of a bacteriocin, pediocin ach, by pediococcus acidilactici h.	the influence of growth parameters on the production of pediocin ach by pediococcus acidilactici h was studied. this strain produced large quantities of pediocin ach in tge broth (trypticase [1%], glucose [1%], yeast extract [1%], tween 80 [0.2%], mn [0.033 mm], mg [0.02 mm] [ph 6.5]) within 16 to 18 h at 30 to 37 degrees c (final ph, 3.6 to 3.7). pediocin ach production was negligible when the ph of the medium was maintained at 5.0 or above, even in the presence of high cell mass.	1991	16348467
assessment of pediococcus acidilactici as a potential silage inoculant.	eighteen pediococcus strains were screened for their potential as silage inoculants. pediococcus acidilactici g24 was found to be the most suitable, exhibiting a short lag phase on both glucose and fructose, a rapid rate of acid production, a high sugar-to-lactate conversion efficiency, no detectable breakdown of proteins or lactic acid, and the ability to grow within a broad range of ph and temperature. when tested in laboratory silos using grass with a water-soluble carbohydrate content of 24 ...	1992	16348773
pharmacokinetic interaction between 1,3-butadiene and styrene in sprague-dawley rats.	gas uptake studies were carried out to evaluate kinetic interactions between 1,3-butadiene and styrene in sprague-dawley rats. the animals were co-exposed by inhalation to a mixture of 1,3-butadiene between 20 and 6000 ppm (v/v) and styrene between 0 and 500 ppm. the data demonstrate that metabolism of 1,3-butadiene was partially inhibited by styrene. the inhibition was competitive at atmospheric concentrations of styrene up to 90 ppm. higher concentrations of styrene resulted in a small additio ...	1992	1610292
collapse of the proton motive force in listeria monocytogenes caused by a bacteriocin produced by pediococcus acidilactici.	the effect of pediocin jd, a bacteriocin produced by pediococcus acidilactici jd1-23, on the proton motive force and proton permeability of resting whole cells of listeria monocytogenes scott a was determined. control cells, treated with trypsin-inactivated bacteriocin at a ph of 5.3 to 6.1, maintained a ph gradient and a membrane potential of approximately 0.65 ph unit and 75 mv, respectively. however, these gradients were rapidly dissipated in cells after exposure to pediocin jd, even though n ...	1992	1444365
purification and amino acid sequence of a bacteriocin produced by pediococcus acidilactici.	a bacteriocin produced by pediococcus acidilactici has been purified to homogeneity by a rapid and simple four-step purification procedure which includes ammonium sulphate precipitation, chromatography with a cation-exchanger and octyl sepharose, and reverse-phase chromatography. the purification resulted in an approximately 80,000-fold increase in the specific activity and about a 6-fold increase in the total activity. the amino acid composition and sequencing data indicated that the bacterioci ...	1992	1402795
nucleotide and amino acid sequence of pap-gene (pediocin ach production) in pediococcus acidilactici h.	n-terminal analysis of purified pediocin ach produced a partial sequence of 23 amino acids. this sequence matched perfectly with a segment of 23 amino acids in a 62 amino acid molecule generated from the 186 nucleotide sequence open reading frame in a hind iii fragment in psmb74 encoding pap-gene (pediocin ach production). it is suggested that the molecule is translated as inactive prepediocin ach of 62 amino acids. then through enzymatic modifications the leader segment of 18 amino acids is rem ...	1992	1368421
on the onset of eye-head coordination in infants.	head movements have been studied in newborns, during a visual pursuit and in relation with ocular movements, in order to know whether head movements help the ocular activity or impede it. the infants were tested three times during the first month of life. they were placed in a seated position, with the head held upright, but free to move. the results provided evidence that eyes and head were directionally coupled at as early as 2 weeks and that the interval between eye and head rotation decrease ...	1992	1388805
characterization and antibiotic susceptibility of pediococcus acidilactici strains isolated from neutropenic patients.		1992	1396762
genomic analysis of pediococcus starter cultures used to control listeria monocytogenes in turkey summer sausage.	the pulsed-field technique of clamped homogeneous electric field electrophoresis was employed to characterize and size genomic dna of three pediocin-producing (ped+) and two non-pediocin-producing (ped-) strains of pediococcus acidilactici. comparison of genomic fingerprints obtained by digestion with the low-frequency-cleavage endonuclease asci revealed identical restriction profiles for four of the five strains analyzed. summation of results for 10 individually sized asci fragments estimated t ...	1992	1444419
the effects of na(+)-k(+)-cl- co-transport and cl(-)-hco3-exchange blockade on the membrane potential and intracellular chloride levels of rat arterial smooth muscle, in vitro.	membrane potential and intracellular chloride levels have been measured simultaneously in vascular smooth muscle from the femoral artery of the rat, in vitro, using double-barrelled chloride-sensitive microelectrodes. these values are compared in the presence and absence of bicarbonate, and inhibitors of cl(-)-hco3- exchange and na(+)-k(+)-cl- co-transport. both dids and bumetanide caused a hyperpolarization of membrane potential (em) and a fall in [cl-]i, in the presence of extracellular hco3-. ...	1992	1489543
cloning, expression, and nucleotide sequence of genes involved in production of pediocin pa-1, and bacteriocin from pediococcus acidilactici pac1.0.	the production of pediocin pa-1, a small heat-stable bacteriocin, is associated with the presence of the 9.4-kbp plasmid psrq11 in pediococcus acidilactici pac1.0. it was shown by subcloning of psrq11 in escherichia coli cloning vectors that pediocin pa-1 is produced and, most probably, secreted by e. coli cells. deletion analysis showed that a 5.6-kbp sali-ecori fragment derived from psrq11 is required for pediocin pa-1 production. nucleotide sequence analysis of this 5.6-kbp fragment indicated ...	1992	1514784
enhanced control of listeria monocytogenes by in situ-produced pediocin during dry fermented sausage production.	to determine whether pediocin is produced and has effective antilisterial activity during food fermentation, six sausage fermentation trials were conducted with antibiotic-resistant, pediocin-producing (bac+) pediococcus acidilactici pac 1.0 (strr rifr) and an isogenic pediocin-negative (bac-) derivative used as a control. meat was inoculated (ca. 10(5) cfu/g) with a composite of five listeria monocytogenes strains, each electrotransformed with pgk12 (cmr emr). p. acidilactici and l. monocytogen ...	1992	1575489
purification and primary structure of pediocin pa-1 produced by pediococcus acidilactici pac-1.0.	the plasmid-encoded bacteriocin pediocin pa-1, produced by the gram-positive bacterium pediococcus acidilactici strain pac-1.0, was purified to homogeneity. the purified product exhibited antibacterial activity against several gram-positive bacterial strains, including the food pathogen listeria monocytogenes. pediocin pa-1 is a 4629-da peptide with 44 amino acids and two disulfide bonds. the amino acid sequence and arrangement of the disulfide bonds were determined. sequence data were used to c ...	1992	1575516
headspace flavour compounds produced by yeasts and lactobacilli during fermentation of preferments and bread doughs.	production of volatile flavour compounds during fermentation with pure cultures of saccharomyces cerevisiae and candida guilliermondii, lactobacillus brevis and lactobacillus plantarum have been investigated, using wheat doughs and several preferements as substrates. for yeast, preferments consisted of 10% (w/v) glucose, maltose and sucrose solutions, whereas for lactobacilli they consisted of supplemented and unsupplemented (3% and 10% (w/v)) glucose solutions, and a 10% (w/v) wheat flour slurr ...	1992	1622751
tert-butyl hydroperoxide-induced radical production in rat liver mitochondria.	when rat liver mitochondria are treated with tert-butyl hydroperoxide (tbhp) in the presence of the spin trap 5,5-dimethyl-1-pyrroline n-oxide (dmpo), electron paramagnetic resonance (epr) signals are detected attributable to spin adducts resulting from the trapping of methyl, tert-butoxyl, and tert-butylperoxyl radicals. the addition of respiratory substrate results in a 3- to 7.5-fold increase in the signal intensity of the dmpo/methyl adduct, no change in the signal intensity of the dmpo/tert ...	1992	1317326
basic counterpoint: mechanisms and pathways of gonadal steroid modulation of growth hormone secretion.	in spite of the different patterns of gh secretion observed in male and female rats, it can be argued that there are limited differences between the mechanism of action of androgens and estrogens as reported in the literature. however, we feel that it is possible to organize the available data into a unique physiological model explaining the sex-based differences in gh secretion in the rat. thus, it can be proposed that the greater spontaneous gh peaks observed in the male with respect to the fe ...	1992	1352244
monoclonal antibody-colony immunoblot method specific for isolation of pediococcus acidilactici from foods and correlation with pediocin (bacteriocin) production.	balb/c mice were immunized with broken, heat-killed cells of pediococcus acidilactici h. after murine cell fusions, one monoclonal antibody (mab), ped-2b2, was selected on the basis of its positive reaction with seven of seven strains tested in an enzyme-linked immunosorbent assay with whole cells of p. acidilactici. the mab ped-2b2 did not show any cross-reactions with other lactic-acid bacteria or other gram-positive or gram-negative organisms. sodium dodecyl sulfate-polyacrylamide gel electro ...	1992	1637167
[an electron microscopic study and the morphofunctional status of the lungs in an experimental reproduction of acute pneumonia].	an analysis of the ultrastructural organization of alveocytes (types i and ii), endotheliocytes, interstitium alveolar, macrophages and aerohematic barrier in rabbits with experimental acute pneumonia revealed disorders of synthesis and release of surfactant on the alveolar surface. the observed in pneumonia edema and swelling of the aerohematic barrier components as well as the interstitium reduces the protective mechanism of alveols favouring transition of the disease to a protracted form.	1992	1448991
[effect of environmental temperature on the course of spontaneous postmortem pupillary changes (studies of the rabbit eye)].	the diameter of the pupils was measured in the bodies of rabbits under different ambient temperatures (2-4 degrees c, 10-12 degrees c, 18-20 degrees c, 28-30 degrees c). it was found that the pupils changed in three phases: initial miosis, which set in within minutes after death, was followed by a mydriatic phase, which lasted several hours and was approximately synchronous with the post-mortem rigidity of the skeletal muscles. in the third phase the pupils slowly contracted in the course of sev ...	1992	1489345
septicemia and hepatic abscess caused by pediococcus acidilactici.	a case of postoperative pediococcus acidilactici septicemia with parallel isolation of the organism from hepatic specimens is presented. laboratory methods to identify this vancomycin-resistant gram-positive cocci are described. very few cases of documented infections due to this bacterium have been reported in the literature.	1992	1396771
[educational goals for specialty continued education and its application in steglitz hospital].		1992	183047
[community health nursing facing new tasks].		1992	183046
nutritional requirements in multiple auxotrophic lactic acid bacteria: genetic lesions affecting amino acid biosynthetic pathways in lactococcus lactis, enterococcus faecium, and pediococcus acidilactici.	in a study of genetic lesions responsible for amino acid requirements in multiple auxotrophic lactic acid bacteria, a systematic attempt was made to isolate mutants that could synthesize each of the amino acids required by the parental strains of lactococcus lactis, enterococcus faecium, and pediococcus acidilactici. after treatment with appropriate mutagens or, in some cases, spontaneously, such mutants could indeed be obtained with respect to many but not all essential amino acids. successful ...	1992	27280812
effects of starter cultures on the formation of flavour compounds in dry sausage.	the purpose of this work was to study the impact of starter cultures on the production of flavour compounds in dry sausages. the effect of six starter cultures corresponding to different combinations of lactic acid bacteria (lactobacillus sake l110, pediococcus acidilactici 725, p. pentosaceus 716) and different staphylococcus species (s. carnosus 833, s. warneri 863, s. saprophyticus m31) strains were tested in a total of 30 dry sausages without spices. the analysis of flavour compounds using a ...	1993	22061221
purification, partial characterization and plasmid-linkage of pediocin sj-1, a bacteriocin produced by pediococcus acidilactici.	pediococcus acidilactici sj-1, isolated from a naturally-fermented meat product, produced an antibacterial agent active against selected strains of lactobacillus spp., clostridium perfringens and listeria monocytogenes. the agent was bactericidal against sensitive indicators, and sensitive to proteolytic enzymes; it was identified as a bacteriocin, and was designated as pediocin sj-1. it was stable over a wide ph range (3-9), and apparently most stable in the lower part of that range. at ph 3.6, ...	1993	8420920
detection, identification and characterization of bacteriocin-producing lactic acid bacteria from retail food products.	forty bacteriocin-producing (bac+) lactic acid bacteria (lab) were isolated from food samples purchased from retail supermarkets and local farms. of the 40 bac+ isolates, 18 were isolated from 85 food samples by enrichment (21% isolation rate) whereas eight were obtained from 63 samples by direct plating (13% isolation rate). by direct plating, bac+ lab were detected at levels up to 2.4 x 10(5) cfu/g in ready-to-eat meats. the bac+ isolates were identified by carbohydrate fermentation patterns, ...	1993	8257654
partial characterization of an rpod-like gene of lactococcus lactis subsp. lactis ml3 with a polymerase chain reaction-based approach.	with degenerated oligonucleotide primers for conserved regions of bacterial sigma factor proteins, a 117-bp internal dna fragment of an rpod-like gene of lactococcus lactis subsp. lactis ml3 was amplified by the polymerase chain reaction (pcr). the dna sequence of this pcr product was determined by cycle sequencing, and the deduced amino acid sequence of this internal fragment showed an extensive homology with the known sigma factor sequences from six other microorganisms and present a 13-amino ...	1993	7764136
fate of listeria monocytogenes and pediococcal starter cultures during the manufacture of chicken summer sausage.	two formulations of chicken summer sausages [100% hand deboned chicken meat (hdcm) and 85% hdcm and 15% chicken hearts (hdcm-ch)] were prepared with a nonpediocin-producing (ped-) pediococcus acidilactici starter culture and inoculated with 10(4) or 10(7) cfu of a five-strain mixture of listeria monocytogenes/g of batter. sausages were fermented to ph 5.0 (11 h), cooked to an internal temperature of 66.5 c, cold-showered, and stored at 4 c (60 days) and 30 c (7 days). for both formulations and i ...	1993	8234138
pediocin pa-1, a bacteriocin from pediococcus acidilactici pac1.0, forms hydrophilic pores in the cytoplasmic membrane of target cells.	pediocin pa-1 is a bacteriocin which is produced by pediococcus acidilactici pac1.0. we demonstrate that pediocin pa-1 kills sensitive pediococcus cells and acts on the cytoplasmic membrane. in contrast to its lack of impact on immune cells, pediocin pa-1 dissipates the transmembrane electrical potential and inhibits amino acid transport in sensitive cells. pediocin interferes with the uptake of amino acids by cytoplasmic membrane vesicles derived from sensitive cells, while it is less effective ...	1993	8285666
characterization and frequency distribution of species of lactic acid bacteria involved in the processing of mawè, a fermented maize dough from benin.	lactic acid bacteria involved in the natural fermentation of both home-produced and commercial mawè were investigated during a 72 h fermentation period. lactobacillus spp. constitute the majority (94%) of the strains of the lactic acid bacteria isolated, among which 89% represent the betabacterium group. they include l. fermentum (biotype cellobiosus) (41%), l. fermentum or l. reuteri (19%), l. brevis (26%), l. confusus (less than 2%), l. curvatus (less than 1%) and l. buchneri (less than 1%). o ...	1993	8347427
antimicrobial susceptibility of pediococcus spp. and genetic basis of macrolide resistance in pediococcus acidilactici hm3020.	we determined the mics of 28 antimicrobial agents against 36 clinical strains of pediococcus spp. (25 p. acidilactici, 9 p. pentosaceus, and 2 p. urinaeequi strains). penicillin g, imipenem, gentamicin, netilmicin, erythromycin, clindamycin, rifampin, chloramphenicol, daptomycin, and ramoplanin were the most active. all strains of p. acidilactici were susceptible to novobiocin, whereas all isolates of p. pentosaceus were resistant. novobiocin could therefore be helpful for differentiation of the ...	1993	8494376