| radiation sensitivity of human ovarian carcinoma cell lines in vitro: effects of growth factors and hormones, basement membrane, and intercellular contact. | the radiation sensitivity of six established human ovarian carcinoma cell lines was determined in vitro under five different experimental conditions. cells from exponentially growing monolayer cultures were assayed under three different post-irradiation culture conditions, i.e., conventional conditions on a plastic surface, in the presence of growth factors and hormones, and on a basement membrane. multicellular aggregates were dissociated either immediately before or immediately after irradiati ... | 1988 | 3182331 |
| postural adjustments in the monkey: effects of velocity on emg sequence. | monkeys were trained to perform flexion or extension movements of the elbow in a strictly restrained position. even in these conditions, postural adjustments were still recorded. they appeared to be organized in a consistent pattern. when trials were ranked with increasing velocity, two kinds of emg changes could be described: (i) those in the upper part of the body which may be triggered by anticipatory postural motor programs; (ii) those in lower limbs recruited only with higher velocities and ... | 1988 | 3126464 |
| follicle-stimulating hormone induces deoxyribonucleic acid polymerase alpha before deoxyribonucleic acid synthesis in immature rat ovarian follicles perfused in vitro. | to examine the effects of follicle-stimulating hormone (fsh) on cell proliferation and cell division in ovaries, deoxyribonucleic acid (dna) polymerase activities and dna concentrations were determined in ovarian follicles of immature, hypophysectomized, estradiol-treated rats perfused in vitro. ovine fsh (2-20 micrograms/ml) significantly enhanced the dna polymerase alpha activity in the follicles perfused for 180-300 minutes, whereas the dna polymerase beta activity and dna concentration showe ... | 1988 | 3126468 |
| [taxonomic study of epiphyte lactic bacteria in grapes in galicia]. | a taxonomic analysis of the malolactic microflora present in nine grape samples from different zones of galicia, was carried out. nineteen strains were isolated and identified as lactobacillus plantarum (42%), l. brevis (10.6%), l. casei (5.25%), l. hilgardii (5.25%), streptococcus cremoris (5.25%), leuconostoc lactis (5.25%), l. oenos (10.6%), and pediococcus acidilactici (15.8%). | 1987 | 3268295 |
| lack of an effect of 6-hydroxydopamine lesions of the nucleus accumbens on intravenous morphine self-administration. | the neurotoxin, 6-hydroxydopamine (6-ohda), has been used to selectively destroy dopamine containing neurons in discrete brain regions. lesions of the nucleus accumbens with this neurotoxin decrease or eliminate cocaine and amphetamine self-administration and either increase or do not affect opiate self-administration in rats with unrestricted access to food and water. this study reports the effects of 6-ohda lesions of the nucleus accumbens on responding maintained by food, water or morphine (3 ... | 1988 | 3147458 |
| fine structure of the a-band in cryo-sections. diversity of m-band structure in chicken breast muscle. | electron micrographs of longitudinal ultrathin cryo-sections and plastic sections of chicken pectoralis muscle together with their average images have been used to study in detail the axial structure of the m-band. it was found that m-band structure could vary markedly in different fibres, even within the white part of the muscle. strong m-band density ("m-bridges") could be seen at m4 and m4' in all fibres. on the other hand the density at m1 or m6 could vary systematically. some fibres (probab ... | 1988 | 3209858 |
| studies on competitive saprophytic colonization of sclerotium rolfsii sacc. causing foot-rot disease of barley under certain pesticidal treatments. | | 1988 | 3255750 |
| in-vitro study of the enkephalinergic hypothesis for non-adrenergic, non-cholinergic innervation in the cat stomach. | it has been suggested that enkephalins are involved in the gastric relaxation induced by stimulation of the non-adrenergic, non-cholinergic vagal fibres in the cat stomach. experiments were therefore performed on strips of cat stomach. with longitudinal and circular gastric fundus and corpus strips from reserpinized cats, non-adrenergic, non-cholinergic relaxatory responses could be elicited by transmural electrical stimulation in tyrode solution containing atropine and 5-hydroxytryptamine. morp ... | 1986 | 2869124 |
| drug permeability of the endolymphatic sac. | intravenously injected kanamycin sulfate (km) was located in the endolymphatic sac (es) of guinea pigs by an immunohistologic method. kanamycin sulfate had passed through the capillaries in the subepithelial layer and reached the epithelial layer as early as 1 minute after injection. the amount of km in the epithelial layer gradually increased with time (until 30 minutes after injection). the km also accumulated in floating cells in the es. these results tend to indicate that the es is rather ea ... | 1987 | 3304088 |
| [clinical application of energy subtraction imaging using digital radiography for excretory urography in infants]. | | 1987 | 3329251 |
| the importance of timing for the action of alpha 1- or beta-agonists: possible relationship with the density of [3h]prazosin and [3h]dihydroalprenolol binding sites. | exploratory behaviour was measured in the open-field at 8.00 h and 20.00 h with naive rats and rats treated with an alpha 1-agonist (phenylephrine, 25 micrograms i.c.v.) or beta-agonists (salbutamol, 2.5 mg/kg; clenbuterol 0.05 mg/kg i.p.). the spontaneous activity was similar at both times, yet the response to a alpha 1-as well as to beta-stimulation was significantly enhanced at 20.00 h. the density of cortical [3h]prazosin and [3h]dihydroalprenolol ([3h]dha) binding sites at 20.00 h was enhan ... | 1985 | 2864268 |
| mapping the effects of motor cortex stimulation on somatosensory relay neurons in the rat thalamus: direct responses and afferent modulation. | single unit recordings were used to map the spatial distribution of motor (mi) cortical influences on thalamic somatosensory relay nuclei in the rat. a total of 215 microelectrode penetrations were made to record single neurons in tracks through the medial and lateral ventroposterior (vpm and vpl), ventrolateral (vl), reticular (nrt), and posterior (po) thalamic nuclei. single units were classified according to their: 1) location within the nuclei, 2) receptive fields, and 3) response to standar ... | 1990 | 2322860 |
| mapping the effects of motor cortex stimulation on somatosensory relay neurons in the rat thalamus: direct responses and afferent modulation. | single unit recordings were used to map the spatial distribution of motor (mi) cortical influences on thalamic somatosensory relay nuclei in the rat. a total of 215 microelectrode penetrations were made to record single neurons in tracks through the medial and lateral ventroposterior (vpm and vpl), ventrolateral (vl), reticular (nrt), and posterior (po) thalamic nuclei. single units were classified according to their: 1) location within the nuclei, 2) receptive fields, and 3) response to standar ... | 1990 | 2322860 |
| protected methionine supplementation with extruded blend of soybeans and soybean meal for dairy cows. | methionine may be the first amino acid limiting milk production in early lactation cows. to evaluate this further, 23 high producing holstein cows (9 multiparous and 14 primiparous) were fed an extruded blend of soybeans and soybean meal (40:60) without or with 15 g of added dl-methionine as 50 g of ruminally protected methionine product during wk 4 to 16 postpartum. cows were fed a 15.8% crude protein total mixed ration consisting of 30% (dry basis) corn silage, 15% alfalfa hay, and 55% concent ... | 1987 | 3571638 |
| pathogenesis of trachoma: the stimulus for inflammation. | active trachoma is characterized by chronic inflammation of the conjunctiva, and repeated episodes of reinfection are thought to be necessary to sustain this inflammation. it is currently believed that much of the tissue damage is immunologically mediated. to identify which antigens might be responsible for stimulating this continued inflammation, cynomolgus monkeys that had recovered from a previous ocular infection with chlamydia trachomatis were challenged with various antigen preparations. p ... | 1987 | 3571982 |
| purification, characterization and antimicrobial spectrum of a bacteriocin produced by pediococcus acidilactici. | an antimicrobial peptide designated pediocin ach was isolated from pediococcus acidilactici strain h. the pediocin ach was purified by ion exchange chromatography. the molecular weight of pediocin ach was determined by sds-page to be about 2700 daltons. pediocin ach was sensitive to proteolytic enzymes resistant to heat and organic solvents, and active over a wide range of ph. pediocin ach exhibited inhibition against several food spoilage bacteria and foodborne pathogens including staphylococcu ... | 1988 | 2906056 |
| regulation of the glucose phosphotransferase system in brochothrix thermosphacta by membrane energization. | uptake of 2-deoxyglucose, alpha-methylglucopyranoside, and glucose into intact cells of brochothrix thermosphacta (formerly microbacterium thermosphactum, atcc 11509) was stimulated by kcn or cccp. the glucose analogs were recovered almost totally as the sugar phosphates. membrane vesicles were isolated from protoplasts and shown to be right side out by freeze fracturing and by using atpase as a marker for the cytoplasmic membrane surface. uptake of glucose into vesicles was dependent on the pre ... | 1985 | 2995314 |
| [experimental and clinical studies of sutures of natural silk produced by oak silkworms]. | | 1986 | 3716085 |
| nacl and ca delivery at the bend of rat deep nephrons decreases during antidiuresis. | the effects of 1-desamino-8-d-arginine vasopressin (ddavp) on superficial and juxtamedullary nephrons were investigated by micropuncture in diabetes insipidus (di) brattleboro rats chronically treated with the peptide. the rats, acutely deprived of endogenous calcitonin, parathyroid hormone (pth), and glucagon [hormone-deprived (hd) rats], were examined either 4 days after cessation of ddavp treatment (hdt, control diuretic rats) or when the treatment was continued until the micropuncture experi ... | 1987 | 3591953 |
| effect of hyponatremia on response to volume depletion in uremic dogs. | it has been suggested that sodium depletion can alter cardiovascular reflexes and vascular sensitivity to pressors. to define the effects of hyponatremia on the hemodynamic response to volume depletion in the setting of uremia, isolated ultrafiltration was performed in acutely uremic dogs previously rendered hyponatremic (127 + 2.5 [sd] meq/l) for 72 hours by dialyzing against a low-sodium dialysate. response was compared with that of the same animals in the normonatremic (150 +/- 3.6 meq/l) con ... | 1987 | 3794518 |
| cardiovascular and metabolic response of tumour-bearing dogs to whole body hyperthermia. | whole body hyperthermia was induced in older, tumour-bearing dogs using a radiant heat device. dogs were anaesthetized (thiopental), paralysed (atracurium), and mechanically ventilated (100 per cent o2) during the heating procedure. heart rate, systolic/diastolic, and mean arterial blood pressure, and arterial blood gases were monitored throughout the procedure. serum biochemical parameters, complete blood count, and coagulation profiles were evaluated before, during, and after whole body hypert ... | 1987 | 3693985 |
| stereochemistry of estrogen biosynthesis by a reconstituted aromatase cytochrome p-450 preparation from human placenta. | according to the literature, the multistep reaction mechanism of estrogen biosynthesis proceeds with stereospecific loss of the equatorial 1 beta-, and axial 2 beta-protons. these results were deduced from experiments carried out, either with crude microsomes, or at best with impure enzyme extracts. however, when [1,2- 3h]4-androstene-3,17-dione of known absolute 3h-label distribution was incubated with a reconstituted enzyme system, consisting of homogeneous nadph-cytochrome p-450 reductase and ... | 1986 | 3707583 |
| comparative effects of caerulein on food intake and pancreatic secretion in dogs. | we compared effects of the cck analog caerulein on feeding and pancreatic secretion. nine fasted mongrel dogs with gastric and pancreatic fistulas received scalar doses of caerulein (0, 6.25, 12.5, 25, 50, 100, 200, 400 pmol/kg-hr, each for 30 min). the d50 dose for stimulation of pancreatic secretion was 15 pmol/kg-hr. effects of intravenous caerulein (0 to 800 pmol/kg-hr; 15 min before and during a 45 min test meal) on food intake were examined in 8 beagles under 4 feeding conditions: 1 meal/d ... | 1986 | 3768748 |
| multiple biochemical deficits in both gray and white matter of alzheimer brains. | a biochemical investigation of 21 brains from patients with dementia of alzheimer type (ad/sdat) and 22 brains from controls was made. in the normal brains 5-hydroxytryptamine (5-ht), noradrenaline (na) and dopamine (da) were reduced with age while their metabolites were not reduced. in the brains from the alzheimer patients the examination of gray matter (caudate nucleus and hippocampus) showed a significant reduced activity of choline-acetyl transferase (cat) and a reduction of acetylcholinest ... | 1986 | 3797685 |
| new aspects of the epidemiology of hepatitis delta virus infection? | | 1986 | 3804179 |
| nucleotide pools of novikoff rat hepatoma cells growing in suspension culture. iv. nucleoside transport in cells depleted of nucleotides by treatment with kcn. | | 1973 | 4346696 |
| an aids patient who died too soon. | | 1985 | 3917452 |
| mechanism of cyclosporin a-induced inhibition of prostacyclin synthesis by macrophages. | in vitro studies of pg production over a 24 h period by adherent rat peritoneal macrophages activated by serum-opsonized zymosan revealed that csa (0.3-10 micrograms/ml) caused a dose-related inhibition of pgi2 (assayed as 6-oxo-pgf1 alpha) formation. indomethacin (ind, 0.01-10 micrograms/ml) and dexamethasone (dex, 0.01-10 micrograms/ml) also inhibited the pg production in a dose-related manner. when arachidonic acid (10 micrograms/ml) was added together with the inhibitors, there was no change ... | 1985 | 3936119 |
| an aids patient who died too soon. | | 1985 | 3917452 |
| mechanism of cyclosporin a-induced inhibition of prostacyclin synthesis by macrophages. | in vitro studies of pg production over a 24 h period by adherent rat peritoneal macrophages activated by serum-opsonized zymosan revealed that csa (0.3-10 micrograms/ml) caused a dose-related inhibition of pgi2 (assayed as 6-oxo-pgf1 alpha) formation. indomethacin (ind, 0.01-10 micrograms/ml) and dexamethasone (dex, 0.01-10 micrograms/ml) also inhibited the pg production in a dose-related manner. when arachidonic acid (10 micrograms/ml) was added together with the inhibitors, there was no change ... | 1985 | 3936119 |
| studies on the lysosomes of l132 cells infected with either rhinovirus type 2 or poliovirus type 1. | | 1974 | 4362408 |
| [staphylococcal antitoxin as an indicator of humoral specific immunity in different forms of staphylococcal diseases in children]. | | 1974 | 4467070 |
| quantitative and temporal analyses of murine antibody response in serum and gut secretions to infection with giardia muris. | we analyzed the appearance and level of giardia muris-specific antibody of immunoglobulin a (iga), igg, and igm isotypes, at weekly intervals, over the course of a 7-week infection in balb/c and c57bl/6 mice. using sensitive immunoradiometric assays, we observed that iga antibody was the only detectable anti-g. muris antibody in intestinal secretions throughout the course of infection. no secreted igg or igm anti-g. muris antibody was detected even in concentrated intestinal secretions. the expu ... | 1986 | 3957428 |
| quantitative and temporal analyses of murine antibody response in serum and gut secretions to infection with giardia muris. | we analyzed the appearance and level of giardia muris-specific antibody of immunoglobulin a (iga), igg, and igm isotypes, at weekly intervals, over the course of a 7-week infection in balb/c and c57bl/6 mice. using sensitive immunoradiometric assays, we observed that iga antibody was the only detectable anti-g. muris antibody in intestinal secretions throughout the course of infection. no secreted igg or igm anti-g. muris antibody was detected even in concentrated intestinal secretions. the expu ... | 1986 | 3957428 |
| csf/shunt infections--the bane of our lives! | thirty one patients were treated for 43 laboratory proven shunt or csf infections, between 1980 to 1986 inclusive. they required 63 separate treatments. infective episodes were grouped retrospectively according to method of treatment employed i.e. 1) placement of external drainage system or 2) reservoir and tapping, 3) intravenous or intraventricular antibiotics alone, 4) total change of shunt prosthesis, 5) partial change of prosthesis or 6) removal of all the prosthesis. see table ii. the sing ... | 1987 | 3433967 |
| the effects of vasectomy on the testis. | the increased use of vasectomy for population control has led to a large number of animal studies on the morphologic, physiologic, and immunologic responses to this procedure. the extent, nature, and timing of morphologic alterations in the testis showed marked species variations and ranged from autoimmune orchitis to an absence of detectable changes. physiologic studies indicated, contrary to expectation, that the hydrostatic pressure was elevated only in the distal part of the epididymis and ... | 1985 | 4058509 |
| [review on internal medicine, 1984. electrolyte disturbance. ii-1. potassium homeostasis and its abnormality]. | | 1985 | 4045285 |
| effect of methyl mercury on prostaglandin synthesis in rabbit kidney medulla slices. | effects of methylmercuric chloride (mmc) in the absence and presence of ascorbic acid on the synthesis of prostaglandins e2 and f2 alpha, and the lipid peroxidation of rabbit kidney medulla slices were studied. mmc showed dose-dependent inhibition of prostaglandins e2 and f2 alpha formation. the lipid peroxidation was not stimulated by mmc alone. ascorbic acid plus mmc were able to stimulate the lipid peroxidation markedly, but the lipid peroxidation induced by ascorbic acid plus mmc had no infl ... | 1986 | 3467401 |
| alterations in hamster cell regulatory mechanisms resulting from abortive infection with an oncogenic adenovirus. | | 1973 | 4571263 |
| the iud and prostaglandins: a review of the evidence. | | 1973 | 4209512 |
| [proliferative activity of experimental brain tumors]. | the method of autoradiography was used to study the parameters of the cell cycle and the intensity of proliferative processes in three transplantable rat brain tumors differing in the duration of the latent period and sensitivity to chemotherapy. the tumors were found to be similar in the duration of the cell cycle and its phases, the mean values of the label index and growth fraction, and were characterized by the irregularity of the proliferative processes within the tumor; the label index was ... | 1987 | 3564792 |
| antimicrobial activity of ro 19-5247 (t-2525), a new oral cephalosporin, tested against 7,745 recent clinical isolates. | the susceptibility testing of 7,745 recent clinical isolates from four medical centers showed ro 19-5247 to be eight- to greater than 64-fold more active than cephalexin against the enterobacteriaceae. ro 19-5247 was comparable with cephalexin in anti-staphylococcal activity (mic50, 4.0 micrograms/ml) and fourfold more active than cefixime. none of the oral cephalosporins were effective (mic50, greater than 32 micrograms/ml) against enterococci, pseudomonas aeruginosa and p. maltophilia. beta-la ... | 1987 | 3568594 |
| isoenzymic differences between culture forms of trypanosoma rangeli, t. cruzi, and t. lewisi. | | 1974 | 4608715 |
| coupling of glycosaminoglycans to agarose beads (sepharose 4b). | 1. heparin, heparan sulphate, chondroitin sulphate and dermatan sulphate were covalently attached to beads of agarose activated by cyanogen bromide. the bond is probably mediated by the amino group of a serine or peptide residue at the reducing end of the polysaccharide chain. 2. the uptake of glycosaminoglycan during the coupling procedure is about 0.9mg/ml of wet gel. however, direct analysis of washed and freeze-dried gels reveals that only about one-third of this amount is firmly attached to ... | 1971 | 4257093 |
| [study with fluorescent concanavalin of carbohydrate sites of membranes in human fetal cells; relationship with cell differentiation]. | | 1971 | 4332360 |
| [the sanarelli-shwartzman phenomenon in the light of modern research. 3]. | | 1967 | 4298510 |
| [the sanarelli-shwartzman phenomenon in the light of modern research. 3]. | | 1967 | 4298510 |
| the role of surface antigens in the protective potency of bordetella pertussis suspensions as measured by the intracerebral challenge technique in mice. | | 1968 | 4318938 |
| [effect of some radiosensitizing agents on the dynamics of 24 na and 42 k in the dog]. | | 1972 | 4341099 |
| [experimental evidence for intramolecular energy transfer in hybrid dna of b. subtilis after irradiation with long wavelength uv]. | | 1972 | 4403595 |
| [studies on the therapy of radiation injury and its dependence on the activity of the erythropoietic system]. | | 1972 | 4344563 |
| pulmonary calcification following smallpox handler's lung. | | 1974 | 4375310 |
| [experimental evidence for intramolecular energy transfer in hybrid dna of b. subtilis after irradiation with long wavelength uv]. | | 1972 | 4403595 |
| the golgi complex in the esophageal mucous glands of the newly hatched chick. | | 1971 | 4257786 |
| alterations in hamster cell regulatory mechanisms resulting from abortive infection with an oncogenic adenovirus. | | 1973 | 4571263 |
| [anti-arrhythmic action of diazepam, methaqualone and meprobamate]. | | 1970 | 4100561 |
| gentamicin in the treatment of equine metritis. | | 1972 | 4486548 |
| when to use injectable vitamin a in livestock and why. | | 1974 | 4496717 |
| [prolonged respiratory assistance with an artificial lung with membranes]. | | 1973 | 4541333 |
| simple method of purification and sequencing of a bacteriocin produced by pediococcus acidilactici ul5. | a bacteriocin produced by a strain of pediococcus acidilactici was successfully purified sequentially by acid extraction (at ph 2) and reverse-phase high-performance liquid chromatography (hplc). cell extracts of derivative strains deficient in bacteriocin production exhibited a similar hplc elution profile to the active extracts except for the two peaks containing bacteriocin activity. the sequence of the antibacterial peptide consisted of 44 amino acid residues of which 42 were identified, and ... | 1994 | 7822227 |
| [proceedings: effects of xylocaine during the hatching period of chicken eggs]. | | 1974 | 4478480 |
| a survey on rats, rat fleas, house-flies and mosquitoes in village rahimabad, lucknow district. | | 1972 | 4145258 |
| some aspects of biotin binding to protein catalyzed by biotin-deficient chicken liver preparations. | | 1974 | 4814148 |
| glucose and octanoate utilization by isolated adult rat heart cells. | | 1974 | 4829540 |
| reversion to the streptococcal state of enterococcal protoplasts, spheroplasts, and l-forms. | a method is described for predetermining whether lysozyme-damaged enterococci grow as either the parent strain or as l-forms. organisms treated with lysozyme grew as l-forms on media solidified with low concentrations of agar, or the damaged cells grew as streptococci on media solidified with high concentrations of gelatin. after induction, some of the l-forms reverted to the parent strain, but most did not during three routine subcultures. continued spontaneous reversion occurred through approx ... | 1970 | 4919988 |
| reversion to the streptococcal state of enterococcal protoplasts, spheroplasts, and l-forms. | a method is described for predetermining whether lysozyme-damaged enterococci grow as either the parent strain or as l-forms. organisms treated with lysozyme grew as l-forms on media solidified with low concentrations of agar, or the damaged cells grew as streptococci on media solidified with high concentrations of gelatin. after induction, some of the l-forms reverted to the parent strain, but most did not during three routine subcultures. continued spontaneous reversion occurred through approx ... | 1970 | 4919988 |
| a study of a new human tumor cell line (rhabdomyosarcoma). | | 1974 | 4425030 |
| [multiplication of lyophilized bcg in the body of the white mouse]. | | 1970 | 4939334 |
| studies on liver regeneration. ii. effects of phenobarbital on the onset and pattern of rat liver regeneration following partial hepatectomy. | | 1971 | 4947050 |
| antimicrobial susceptibility of pediococcus spp. and genetic basis of macrolide resistance in pediococcus acidilactici hm3020. | we determined the mics of 28 antimicrobial agents against 36 clinical strains of pediococcus spp. (25 p. acidilactici, 9 p. pentosaceus, and 2 p. urinaeequi strains). penicillin g, imipenem, gentamicin, netilmicin, erythromycin, clindamycin, rifampin, chloramphenicol, daptomycin, and ramoplanin were the most active. all strains of p. acidilactici were susceptible to novobiocin, whereas all isolates of p. pentosaceus were resistant. novobiocin could therefore be helpful for differentiation of the ... | 1993 | 8494376 |
| [closure of urinary tract fistula in experiments and in the clinic with the aid of lyophilized human dura]. | | 1970 | 4947327 |
| gentamicin in the treatment of equine metritis. | | 1972 | 4486548 |
| [role of the parietal associative cortex in the conditioned-reflex activity of rhesus monkeys]. | | 1971 | 5004228 |
| the use of tissue cultures for the separation of v and s influenza antigen. | | 1967 | 4977285 |
| the 1970-71 avian cholera epornitic's impact on certain species. | | 1972 | 5007903 |
| an ultrastructural study of the membranes of keratinizing wool follicle cells. | | 1972 | 5071883 |
| plasmid transfer in pediococcus spp.: intergeneric and intrageneric transfer of pip501. | transfer of the broad-host-range resistance plasmid pip501 from streptococcus faecalis to pediococcus pentosaceus and pediococcus acidilactici occurred between cells immobilized on nitrocellulose filters in the presence of dnase. expression of the pip501-linked erythromycin and chloramphenicol resistance determinants was observed in transconjugants. intrageneric transfer of pip501 from a p. pentosaceus donor to various pediococcal recipients occurred at frequencies of 10(-4) to 10(-7) transconju ... | 1983 | 6311098 |
| [demonstration of cytoadherence properties of cells from the hemolymph of biomphalaria glabrata parasitized by schistosoma mansoni]. | | 1973 | 4122547 |
| production of pediocin ach by lactobacillus plantarum whe 92 isolated from cheese. | among 1,962 bacterial isolates from a smear-surface soft cheese (munster cheese) screened for activity against listeria monocytogenes, six produced antilisterial compounds other than organic acids. the bacterial strain whe 92, which displayed the strongest antilisterial effect, was identified at the dna level as lactobacillus plantarum. the proteinaceous nature, narrow inhibitory spectrum, and bactericidal mode of action of the antilisterial compound produced by this bacterium suggested that it ... | 1996 | 8953710 |
| isolation and characterization of pediocin l50, a new bacteriocin from pediococcus acidilactici with a broad inhibitory spectrum. | lactic acid bacteria were isolated from spanish dry-fermented sausages and screened for bacteriocin production. about 10% of the isolates produced antimicrobial substances when grown on solid media, but only 2% produced detectable activity in liquid media. strain l50, identified as pediococcus acidilactici, showed the strongest inhibitory activity and was active against members of all of the gram-positive genera tested. the strain produced a heat-stable bacteriocin when grown at 8 to 32 degrees ... | 1995 | 7618877 |
| expression of lactococcin a and pediocin pa-1 in heterologous hosts. | pediocin pa-1 production, immunity and secretion are specified by a cluster of four genes in pediococcus acidilactici pac1.0. the production by, secretion of, and immunity to lactococcin a of lactococcus lactis are also determined by four genes. here, expression of the pediocin operon in lactococcus lactis is reported, which could only be achieved by placing it under control of a lactococcal promoter. expression of the lactococcin a operon in pediococcus is also described: recombinant clones of ... | 1995 | 7576505 |
| new evidence for an -andrenergic component in the sympathetic centres: centrally mediated decrease in sympathetic tone by l-dopa and its antagonism by piperoxane and yohimbine. | | 1972 | 5026404 |
| [isolation of a new salmonella (s.ii 41:b:1,5) as well as arizona serotypes (arizona 21:24:25) from a viper (vipera russellii)]. | | 1973 | 4585240 |
| influence of pyridine and some pyridine derivatives on spectral properties of reduced microsomes and on microsomal drug metabolizing activity. | | 1974 | 4824776 |
| immunoperoxidase localization of antihapten antibodies in rats responding unequally to antigenic stimulation. | | 1972 | 5037425 |
| pediococcus acidilactici ldhd gene: cloning, nucleotide sequence, and transcriptional analysis. | the gene encoding d-lactate dehydrogenase was isolated on a 2.9-kb insert from a library of pediococcus acidilactici dna by complementation for growth under anaerobiosis of an escherichia coli lactate dehydrogenase and pyruvate-formate lyase double mutant. the nucleotide sequence of ldhd encodes a protein of 331 amino acids (predicted molecular mass of 37,210 da) which shows similarity to the family of d-2-hydroxyacid dehydrogenases. the enzyme encoded by the cloned fragment is equally active on ... | 1995 | 7539419 |
| cloning, nucleotide sequence, and transcriptional analysis of the pediococcus acidilactici l-(+)-lactate dehydrogenase gene. | recombinant plasmids containing the pediococcus acidilactici l-(+)-lactate dehydrogenase gene (ldhl) were isolated by complementing for growth under anaerobiosis of an escherichia coli lactate dehydrogenase-pyruvate formate lyase double mutant. the nucleotide sequence of the ldhl gene predicted a protein of 323 amino acids showing significant similarity with other bacterial l-(+)-lactate dehydrogenases and especially with that of lactobacillus plantarum. the ldhl transcription start points in p. ... | 1995 | 7887607 |
| analysis of the pediocin ach gene cluster from plasmid psmb74 and its expression in a pediocin-negative pediococcus acidilactici strain. | the 3,500-bp pap operon in the 8,877-bp plasmid psmb74 contains a cluster of four genes, papabcd, of which papa encodes prepediocin (a. m. motlagh, m. bukhtiyarova, and b. ray, lett. appl. microbiol. 18:305-312, 1994). the cluster without the promoter was cloned in the shuttle vector phps9. an escherichia coli strain and a pediocin-sensitive pediococcus acidilactici strain transformed with the recombinant plasmid, pmbr1.0, produced pediocin ach. deletion analysis by introducing mutations in the ... | 1994 | 7944372 |
| monoclonal antibody-based enzyme immunoassay for pediocins of pediococcus acidilactici. | monoclonal antibody (mab) r2-ar against pediocin rs2 was developed. mice were immunized for 12 weeks with pediocin rs2 conjugated to a polyacrylamide gel. two hybridoma fusions yielded an mab that in western blots (immunoblots) reacted only with pediocins rs2 and ach (3 kda) from pediococcus acidilactici rs2 and h, respectively, and did not react with any other bacteriocin, including sakacin a from lactobacillus sake lb 706, leuconocin lcm1 from leuconostoc carnosum lm1, nisin from lactococcus l ... | 1994 | 8085814 |
| identification of lactic acid bacteria from chili bo, a malaysian food ingredient. | ninety-two strains of lactic acid bacteria (lab) were isolated from a malaysian food ingredient, chili bo, stored for up to 25 days at 28 degreesc with no benzoic acid (product a) or with 7,000 mg of benzoic acid kg-1 (product b). the strains were divided into eight groups by traditional phenotypic tests. a total of 43 strains were selected for comparison of their sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) whole-cell protein patterns with a sds-page database of lab. iso ... | 1999 | 9925588 |
| fate of listeria monocytogenes and pediococcal starter cultures during the manufacture of chicken summer sausage. | two formulations of chicken summer sausages [100% hand deboned chicken meat (hdcm) and 85% hdcm and 15% chicken hearts (hdcm-ch)] were prepared with a nonpediocin-producing (ped-) pediococcus acidilactici starter culture and inoculated with 10(4) or 10(7) cfu of a five-strain mixture of listeria monocytogenes/g of batter. sausages were fermented to ph 5.0 (11 h), cooked to an internal temperature of 66.5 c, cold-showered, and stored at 4 c (60 days) and 30 c (7 days). for both formulations and i ... | 1993 | 8234138 |
| pediocin pa-1, a bacteriocin from pediococcus acidilactici pac1.0, forms hydrophilic pores in the cytoplasmic membrane of target cells. | pediocin pa-1 is a bacteriocin which is produced by pediococcus acidilactici pac1.0. we demonstrate that pediocin pa-1 kills sensitive pediococcus cells and acts on the cytoplasmic membrane. in contrast to its lack of impact on immune cells, pediocin pa-1 dissipates the transmembrane electrical potential and inhibits amino acid transport in sensitive cells. pediocin interferes with the uptake of amino acids by cytoplasmic membrane vesicles derived from sensitive cells, while it is less effective ... | 1993 | 8285666 |
| characterization and determination of origin of lactic acid bacteria from a sorghum-based fermented weaning food by analysis of soluble proteins and amplified fragment length polymorphism fingerprinting. | the group that includes the lactic acid bacteria is one of the most diverse groups of bacteria known, and these organisms have been characterized extensively by using different techniques. in this study, 180 lactic acid bacterial strains isolated from sorghum powder (44 strains) and from corresponding fermented (93 strains) and cooked fermented (43 strains) porridge samples that were prepared in 15 households were characterized by using biochemical and physiological methods, as well as by analyz ... | 2000 | 10698775 |
| [microbiological and clinical studies of vancomycin resistant leuconostoc spp. and pediococcus spp. isolated from septicemia patients]. | we described three septicemia cases in which blood cultures yielded gram-positive cocci identified as leuconostoc spp. and pediococcus spp. patients were three male adults aged 63 to 71 years with severe underlying diseases, pancreatic cancer, esophageal cancer and diabetes mellitus with chronic renal failure. they had fever and chills at the onsets of septicemia with acute obstructive suppurative cholangitis, acute pneumonia, and infection complicated with invasion sites of esophageal cancer co ... | 1994 | 7963799 |
| cloning and expression of the pediocin operon in streptococcus thermophilus and other lactic fermentation bacteria. | production of pediocin in pediococcus acidilactici is associated with pmbr1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. these four genes are organized as an operon under control of a single promoter. we have constructed shuttle vectors that contain all four structural genes, the chromosomal promoter st(p2201) from streptococcus thermophilus, and repa from the 2-kbp s. thermophilus plasmid per8. the recombinant plasmi ... | 1999 | 10489440 |
| detection, identification and characterization of bacteriocin-producing lactic acid bacteria from retail food products. | forty bacteriocin-producing (bac+) lactic acid bacteria (lab) were isolated from food samples purchased from retail supermarkets and local farms. of the 40 bac+ isolates, 18 were isolated from 85 food samples by enrichment (21% isolation rate) whereas eight were obtained from 63 samples by direct plating (13% isolation rate). by direct plating, bac+ lab were detected at levels up to 2.4 x 10(5) cfu/g in ready-to-eat meats. the bac+ isolates were identified by carbohydrate fermentation patterns, ... | 1993 | 8257654 |
| characterization and frequency distribution of species of lactic acid bacteria involved in the processing of mawè, a fermented maize dough from benin. | lactic acid bacteria involved in the natural fermentation of both home-produced and commercial mawè were investigated during a 72 h fermentation period. lactobacillus spp. constitute the majority (94%) of the strains of the lactic acid bacteria isolated, among which 89% represent the betabacterium group. they include l. fermentum (biotype cellobiosus) (41%), l. fermentum or l. reuteri (19%), l. brevis (26%), l. confusus (less than 2%), l. curvatus (less than 1%) and l. buchneri (less than 1%). o ... | 1993 | 8347427 |
| purification, partial characterization and plasmid-linkage of pediocin sj-1, a bacteriocin produced by pediococcus acidilactici. | pediococcus acidilactici sj-1, isolated from a naturally-fermented meat product, produced an antibacterial agent active against selected strains of lactobacillus spp., clostridium perfringens and listeria monocytogenes. the agent was bactericidal against sensitive indicators, and sensitive to proteolytic enzymes; it was identified as a bacteriocin, and was designated as pediocin sj-1. it was stable over a wide ph range (3-9), and apparently most stable in the lower part of that range. at ph 3.6, ... | 1993 | 8420920 |
| functional analysis of the pediocin operon of pediococcus acidilactici pac1.0: pedb is the immunity protein and pedd is the precursor processing enzyme. | the bacteriocin pediocin pa-1 operon of pediococcus acidilactici pac1.0 encompasses four genes: peda, pedb, pedc and pedd. transcription of the operon results in the formation of two overlapping transcripts, probably originating from a single promoter upstream of peda. the major transcript comprises peda, pedb, and pedc, while a minor transcript encompasses all of these genes and pedd. by deletion analysis and overexpression of pedb in pediococcus pentosaceus we demonstrate that this gene encode ... | 1995 | 8559070 |
| pediocin 5 production and plasmid stability during continuous free and immobilized cell cultures of pediococcus acidilactici ul5. | continuous production of pediocin 5 from pediococcus acidilactici ul5 was investigated in mrs medium at different ph and dilution rates during continuous free cell (fc) and immobilized cell (ic) fermentations. pediocin 5 activity from fc operated at a dilution rate of 0.31 h-1 largely increased from 128 to 2048 au ml-1 as ph decreased from 7.0 to 5.0. pediocin 5 activity in ic at a dilution rate of 0.93 h-1 was much less affected by ph, varying from 256 au ml-1 at ph 7.0 to 512 au ml-1 at ph 5.0 ... | 1996 | 8698665 |