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screening of lactic acid bacteria and bifidobacteria for potential probiotic use in iberian dry fermented sausages.the purpose of this study was to select lactic acid bacteria and bifibobacteria strains as potential probiotic cultures during the processing of iberian dry fermented sausages. a total of 1000 strains were isolated from iberian dry fermented sausages (363), and human (337) and pig faeces (300) in different culture media. around 30% of these strains, mainly isolated from iberian dry fermented sausages in lamvab agar, were pre-selected for testing as potential probiotics by their ability to grow a ...200822063588
effect of processing methods and starter culture (staphylococcus xylosus and pediococcus pentosaceus) on proteolytic changes in turkish sausages (sucuk) during ripening and storage.proteolytic changes in turkish sausages (sucuk) produced by two methods (heat processing and traditional) were determined during processing and storage for 90 days. the sausages were produced with or without starter culture in both methods. a mixture of staphylococcus xylosus and pediococcus pentosaceus was used as starter culture for their acidic and proteolytic characteristics. the major changes in proteolytic characteristics of sucuk took place during the fermentation stage, with an increase ...200822063339
molecular and technological characterization of lactic acid bacteria from traditional fermented sausages of basilicata region (southern italy).lactic acid bacteria (lab) from traditional fermented sausages of the basilicata region were investigated by ardra-pcr and rapd-pcr for taxonomic identification at species and strain level and characterized on the basis of the growth and acidification at different temperatures, incubation times, levels of nacl and kno(2), hydrolysis of sarcoplasmatic and myofibrillar proteins and antimicrobial, peptide/amino acid release and nitrate reductase activities. lactobacillus sakei was the predominant s ...200822063864
diversity of lactic acid bacteria in two flemish artisan raw milk gouda-type cheeses.pcr-denaturing gradient gel electrophoresis (pcr-dgge) was used to study the diversity of lactic acid bacteria (lab) in two flemish artisan raw milk gouda-type cheeses. in parallel, conventional culturing was performed. isolates were identified using (gtg)(5)-pcr and sequence analysis of 16s rrna and phes genes. discriminant analysis revealed some differences in overall lab diversity between the two batches and between the two cheeses. within each batch, the diversity of 8- and 12-week-old chees ...200818721684
purification of pediocin pa-1 by immunoaffinity chromatography.a one-step purification procedure for purifying pediocin pa-1, a class iia bacteriocin produced by pediococcus acidilactici ul5, was developed based on column immunoaffinity chromatography with specific antipediocin pa-1 polyclonal antibodies coupled to cyanogen bromide-activated sepharosetm. about 13.3 microg/ml purified pediocin pa-1 was obtained from 15 ml p. acidilactici ul5 culture supernatant, as measured by enzyme-linked immunosorbent assay. the specific activity and average recovery of t ...200818727543
novel extracellular proteolytic activity in pediococcus acidilactici atcc 8042.proteolytic systems are common in lactic acid bacteria, but there are few reports about proteases or peptidases in the genus pediococcus. to evaluate the presence of these types of enzymes, pediococcus acidilactici atcc 8042 was cultured in mrs broth. supernatants collected during the log phase showed proteolytic activity towards an elastin dispersion when assayed using a spectrophotometer. zn2+ showed a stimulatory effect, and the proteolytic activity reached its maximum when 200 mmol/l nacl wa ...200818772932
rapid identification of the three major species of dairy obligate heterofermenters lactobacillus brevis, lactobacillus fermentum and lactobacillus parabuchneri by species-specific duplex pcr.in this study, the biodiversity of 154 strains of lactic acid bacteria, including 112 dairy product isolates presumptively identified as obligately heterofermentative lactobacilli (ohl) by classical microbiological tests, as well as 23 ohl-type strains, was investigated by pcr-based methods and gene sequencing. using these techniques, 51% of the cheese isolates were actually identified as ohl. the non-ohl isolates were identified to the leuconostoc, lactobacillus, weisella, pediococcus or strept ...200818510560
isolation and partial characterization of a bacteriocin produced by pediococcus pentosaceus k23-2 isolated from kimchi.screening and partial characterization of a bacteriocin produced by pediococcus pentosaceus k23-2 isolated from kimchi, a traditional korean fermented vegetable.200818540969
antimicrobial activity of enterococcus faecium l50, a strain producing enterocins l50 (l50a and l50b), p and q, against beer-spoilage lactic acid bacteria in broth, wort (hopped and unhopped), and alcoholic and non-alcoholic lager beers.enterococcus faecium l50 produces enterocin l50 (l50a and l50b) (entl50, entl50a and entl50b), enterocin p (entp) and enterocin q (entq) and displays a broad antimicrobial spectrum against the most relevant beer-spoilage lactic acid bacteria (lab) (i.e., lactobacillus brevis and pediococcus damnosus), which is mainly due to the production of entl50 (entl50a and entl50b). bacteriocin assays using in vitro-synthesized entl50 (entl50a and entl50b) showed that both individual peptides possess antimi ...200818544465
a factory-scale application of secondary adjunct cultures selected from lactic acid bacteria during puzzone di moena cheese ripening.the lactic acid populations of 2 seasonal puzzone di moena cheeses made from winter and summer raw cow's milk were characterized at different ripening times. lactic acid bacteria (lab) were isolated on selective media and subjected to genetic typing and identification. the species most frequently found during ripening were lactobacillus paracasei ssp. paracasei, lactobacillus plantarum, and pediococcus pentosaceus. the different strains recognized by random amplification of polymorphic dna-pcr w ...200818650274
selection and use of autochthonous mixed starter for lactic acid fermentation of carrots, french beans or marrows.strains of leuconostoc mesenteroides, lactobacillus plantarum, weissella soli/weissella koreensis, enterococcus faecalis, pediococcus pentosaceus and lactobacillus fermentum were identified from raw carrots, french beans and marrows by partial 16s rrna gene sequence. l. plantarum m1, leuc. mesenteroides c1 and p. pentosaceus f4 were selected based on the rates of growth and acidification in vegetable juice media, and used as the autochthonous mixed starter for the fermentation of carrots, french ...200818710789
regulation of hdc expression and hdc activity by enological factors in lactic acid bacteria.the aim of this work was to study the influence of enological factors on the histidine decarboxylase gene (hdc) expression and on histidine decarboxylase enzyme (hdc) activity in lactobacillus hilgardii, pediococcus parvulus and oenococcus oeni.200818713288
functionality of lactic acid bacteria peptidase activities in the hydrolysis of gliadin-like fragments.to evaluate the role of the peptidase activities from sourdough lactic acid bacteria (lab) in the degradation of alpha-gliadin fragments.200819146533
identification of novel hora-harbouring bacteria capable of spoiling beer.an atp-binding cassette (abc) multi-drug resistance (mdr) gene was found in 4 gram-positive bacterial isolates of environmental origin and found capable of spoiling beer. the bacteria isolated were bacillus cereus, bacillus licheniformis, paenibacillus humicus, and staphylococcus epidermidis; all of which were previously unappreciated as beer-spoilage bacteria. the mdr gene found in these bacteria has less than 37% similarity to known abc mdr proteins described for bacillus and staphylococcus, a ...200818389005
characterization of gtf, a glucosyltransferase gene in the genomes of pediococcus parvulus and oenococcus oeni, two bacterial species commonly found in wine."ropiness" is a bacterial alteration in wines, beers, and ciders, caused by beta-glucan-synthesizing pediococci. a single glucosyltransferase, gtf, controls ropy polysaccharide synthesis. in this study, we show that the corresponding gtf gene is also present on the chromosomes of several strains of oenococcus oeni isolated from nonropy wines. gtf is surrounded by mobile elements that may be implicated in its integration into the chromosome of o. oeni. gtf is expressed in all the gtf(+) strains, ...200818469121
the effect of probiotics and organic acids on shiga-toxin 2 gene expression in enterohemorrhagic escherichia coli o157:h7.probiotics are known to have an inhibitory effect against the growth of various foodborne pathogens, however, the specific role of probiotics in shiga-toxin-producing escherichia coli (stec) virulence gene expression has not been well defined. shiga toxins are members of a family of highly potent bacterial toxins and are the main virulence marker for stec. shiga toxins inhibit protein synthesis in eukaryotic cells and play a role in hemorrhagic colitis and hemolytic uremic syndrome. stec possess ...200818328583
safety of probiotics: translocation and infection.the long history of safety has contributed to the acceptance of probiotics as a safe food adjunct. consequently, many probiotic products and their applications have been granted gras (generally regarded as safe) status. however, this classification has been frequently generalized for all probiotic strains regardless of their application. cases of probiotics from the genera lactobacillus, leuconostoc, pediococcus, enterococcus, and bifidobacterium have been isolated from infection sites, leading ...200818366533
isolation and characterization of bacteriocin-producing bacteria from the gastrointestinal tract of broiler chickens for probiotic use.to isolate and characterize the bacteriocin-producing bacteria (bpb) from the gastrointestinal tract of broiler chickens for probiotic use.200819120666
nisin antimicrobial activity and structural characteristics at hydrophobic surfaces coated with the peo-ppo-peo triblock surfactant pluronic f108.the antimicrobial peptide nisin has been observed to preferentially locate at surfaces coated with the poly[ethylene oxide]-poly[propylene oxide]-poly[ethylene oxide] (peo-ppo-peo) surfactant pluronic f108, to an extent similar to its adsorption at uncoated, hydrophobic surfaces. in order to evaluate nisin function following its adsorption to surfaces presenting pendant peo chains, the antimicrobial activity of nisin-loaded, f108-coated polystyrene microspheres and f108-coated polyurethane cathe ...200818377919
diversity of predominant lactic acid bacteria associated with cocoa fermentation in nigeria.the fermentation of cocoa relies on a complex succession of bacteria and filamentous fungi, all of which can have an impact on cocoa flavor. so far, few investigations have focused on the diversity of lactic acid bacteria involved in cocoa fermentation, and many earlier investigations did not rely on polyphasic taxonomical approaches, which take both phenotypic and genotypic characterization techniques into account. in our study, we characterized predominant lactic acid bacteria from cocoa ferme ...200818213481
rapid quantifiable assessment of nutritional parameters influencing pediocin production by pediococcus acidilactici nrrl b5627.a direct plate bioassay procedure was applied for rapid and quantifiable assessment of the influence of various nutritional parameters on pediocin production by pediococcus acidilactici nrrl b5627. solid-state cultivation of the microorganism was done on mrs-based media over 3-and 6-hours incubation periods. nutritional parameters assessed included the carbon source (glucose, sucrose, fructose, galactose, glycerol), and various salts (nh(4)po(4), cacl(2), kh(2)po(4), mnso(4).h(2)o). glucose was ...200818215516
detection of biogenic amine producer bacteria in a typical italian goat cheese.the aim of this study was to research decarboxylating bacterial strains and biogenic amine content in a typical italian goat cheese (robiola di roccaverano). the study was performed on fresh and ripened samples of goat cheese manufactured from industrial and artisanal producers. sixty-seven bacterial strains isolated showed decarboxylating activity, and enterococcus faecalis was the most widespread decarboxylating species in all artisanal and industrial products. pediococcus acidilactici and ent ...200818236686
phenotypic and genotypic identification of lactic acid bacteria isolated from ethnic fermented bamboo tender shoots of north east india.mesu, soidon, soibum and soijim are ethnic fermented bamboo tender shoot products prepared by the people in north east india. microbiological analysis of mesu, soidon, soibum and soijim showed the population dominated by lactic acid bacteria (lab) ranging up to 10(8) cfu g(-1). the phenotypic characterisation of predominant lab isolated from the fermented bamboo shoot products was based on general morphology, physiological tests, api and biolog systems. the genotypic characterisation of lab was ...200818036695
pediocin sa-1, an antimicrobial peptide from pediococcus acidilactici nrrl b5627: production conditions, purification and characterization.fermentation broths of pediococcus acidilactici nrrl b5627 exhibited a certain antimicrobial activity due to a bacteriocin produced during early growth and until the stationary phase of growth was reached (at optimum of 60% dissolved oxygen saturation). its size was determined by electrospray ionization mass spectrometric analysis as 3.660 kda. n-terminal sequencing showed that the bacteriocin had 19 amino acid residues in the order kyygxngvxtxgkhsxvdx. the purified bacteriocin is similar to ped ...200818093831
succession of dominant and antagonistic lactic acid bacteria in fermented cucumber: insights from a pcr-based approach.the goal of the investigation was to study the succession of major groups of lactic acid bacteria (lab) and their antagonism in salt-fermented cucumber using pcr. in a direct detection method as well as a short enrichment process, pcr enabled detection of leuconostoc and lactobacillus during early hours of fermentation. subsequently, lactobacillus and pediococcus emerged as the dominant genera. nucleic acid sequence of culture-independent clones confirmed the detection of pediococcus as a domina ...200818206770
effects of the administration of pediococcus acidilactici to laying hens on productive performance. 200818685970
pediococcus pentosaceus nb-17 for probiotic use.the plant-derived pediococcus pentosaceus nb-17 was isolated from japanese traditional vegetable pickles. the production of cytokines from mouse spleen cells co-cultivated with heat-killed bacteria was investigated in vitro. the bacteria significantly induced secretion levels of interferon (ifn)-gamma and interleukin (il)-12 p70, and suppressed il-4 productions in ovalbumin (ova) sensitized mouse spleen cells. therefore, the bacteria could effectively stimulate immune activities and showed aller ...200818691534
biodiversity of the microbial community in a spanish farmhouse cheese as revealed by culture-dependent and culture-independent methods.the microbial diversity within alberquilla cheese, made from a spontaneously fermented mixture of raw goats' and sheep's milk in the alpujarra mountains (granada, south-east spain), has been studied by the classical culturing method and also by molecular analysis of community dna. a collection of 206 isolates was obtained from the cheese on different selective/differential media, which were then re-grouped to 52 after randomly amplified polymorphic dna (rapd)-pcr analyses. isolates on man-rogosa ...200818692931
screening from pickled vegetables the potential probiotic strains of lactic acid bacteria able to inhibit the salmonella invasion in mice.to screen from pickled vegetables the potential probiotic lactic acid bacteria (lab) strains with antagonistic activity against salmonella invasion in host.200817927755
role of hypermutability in the evolution of the genus oenococcus.oenococcus oeni is an alcohol-tolerant, acidophilic lactic acid bacterium primarily responsible for malolactic fermentation in wine. a recent comparative genomic analysis of o. oeni psu-1 with other sequenced lactic acid bacteria indicates that psu-1 lacks the mismatch repair (mmr) genes muts and mutl. consistent with the lack of mmr, mutation rates for o. oeni psu-1 and a second oenococcal species, o. kitaharae, were higher than those observed for neighboring taxa, pediococcus pentosaceus and l ...200817993526
functional properties of selected starter cultures for sour maize bread.this paper focuses on the functional properties of maize sour-dough microflora selected and tested for their use as starter cultures for sour maize bread. lactic acid bacteria and yeasts isolated from spontaneously fermented maize dough were selected based on dominance during fermentation and presence at the end of fermentation. functional properties examined included acidification, leavening and production of some antimicrobial compounds in the fermenting matrix. the organisms previously identi ...200818456117
[study of genetic control of pediocins synthesis].plasmid profiles of 2 strains of pediococcus acidilactici and 3 strains of p. pentosaceus were studied. synthesis of bacteriocins in 2 strains of p. acidilactici was coded on the plasmid which was confirmed by experiment with elimination of plasmids and subsequent electrophoregram, which demonstrated synchronous disappearance of bacteriocins synthesis characteristic in plasmid-lacking variants. in 3 strains of p. pentosaceus plasmids were not found which points to chromosomal localization of gen ...200819189461
identification of lactic acid bacteria and gram-positive catalase-positive cocci isolated from naturally fermented sausage (sucuk).the aim of the study was to identify lactic acid bacteria and gram-positive catalase-positive cocci isolated from turkish dry fermented sausage (sucuk) produced by 7 different manufacturers without using starter culture. a total of 129 isolates of lactic acid bacteria were identified phenotypically. lactobacillus plantarum was the dominant species (45.7%) followed by l. curvatus (10.9%) and l. fermentum (9.3%). pediococcus isolates were identified as p. pentosaceus and p. acidilactici. all the i ...200819019118
oligonucleotide microarrays for the detection and identification of viable beer spoilage bacteria.the design and evaluation of an oligonucleotide microarray in order to detect and identify viable bacterial species that play a significant role in beer spoilage. these belong to the species of the genera lactobacillus, megasphaera, pediococcus and pectinatus.200818785882
use of a species-specific multiplex pcr for the identification of pediococci.in this study, the 23s rrna genes of nine different pediococcus type strains were sequenced. by using a multiple sequence alignment with 23s rdna sequences of related lactic acid bacteria two primer pairs were constructed, one for the general identification of the genus pediococcus and one for the identification of the atypical species, p. dextrinicus. furthermore, a primer set for a rapid multiplex pcr identification of the eight typical pediococcus species was developed. with this technique, t ...200818835501
microbial diversity of traditional vietnamese alcohol fermentation starters (banh men) as determined by pcr-mediated dgge.the diversity of fungi and bacteria associated with traditional vietnamese alcohol fermentation starters (banh men) was investigated by pcr-mediated dgge. from 52 starter samples, 13 species of fungi (including yeasts) and 23 species of bacteria were identified. the fungal composition of the starters was consistent with little variation among samples. it consisted of amylase producers (rhizopus oryzae, r. microsporus, absidia corymbifera, amylomyces sp., saccharomycopsis fibuligera), ethanol pro ...200818838186
pediococcus argentinicus sp. nov. from argentinean fermented wheat flour and identification of pediococcus species by phes, rpoa and atpa sequence analysis.a gram-positive, small coccus-shaped lactic acid bacterium, strain lmg 23999(t), was isolated from argentinean wheat flour. 16s rrna gene sequence analysis revealed that the phylogenetic position of the novel strain was within the genus pediococcus, with pediococcus stilesii, pediococcus pentosaceus and pediococcus acidilactici as its closest relatives (97.7, 97.3 and 96.9 % gene sequence similarity, respectively). fluorescent amplified fragment length polymorphism fingerprinting of whole genome ...200819060081
purification and identification of the pediocin produced by pediococcus acidilactici mm33, a new human intestinal strain.the aim of this study was to purify and identify the bacteriocin produced by pediococcus acidilactici mm33, a strain previously isolated from human gut.200817927747
lactic acid bacteria with potential to eliminate fungal spoilage in foods.to investigate antifungal activity produced by lactic acid bacteria (lab) isolated from malted cereals and to determine if such lab have the capacity to prevent fungal growth in a particular food model system.200817976175
high frequency of histamine-producing bacteria in the enological environment and instability of the histidine decarboxylase production phenotype.lactic acid bacteria contribute to wine transformation during malolactic fermentation. they generally improve the sensorial properties of wine, but some strains produce histamine, a toxic substance that causes health issues. histamine-producing strains belong to species of the genera oenococcus, lactobacillus, and pediococcus. all carry an hdca gene coding for a histidine decarboxylase that converts histidine into histamine. for this study, a method based on quantitative pcr and targeting hdca w ...200818065614
growth control of listeria innocua 2030c on vacuum-packaged cold-smoked salmon by lactic acid bacteria.five bacteriocin-producing lactic acid bacteria (lab): enterococcus faecium et05, lactobacillus curvatus et06, l. curvatus et30, l. deldrueckii et32 and pediococcus acidilactici et34, selected by their capacity for growth and producing inhibition in vitro at high salt-on-water content, low temperature and anaerobic atmosphere, conditions simulating cold-smoked fish, were inoculated onto salmon fillets, in co-culture with listeria innocua 2030c, and cold-smoked processed (dry salted for 6 h; dryi ...200818068846
method for reliable isolation of lactobacillus sakei strains originating from tunisian seafood and meat products.in tunisia, several food products derived from meat or seafood are naturally processed, without any addition of bacterial starters. such fermented, dried-cured, salted, or marinated products, as well as the raw meat or fish may thus provide a source to isolate the natural microflora colonizing such environments. we isolated lactic acid bacteria from a representative range of flesh-foods sold or manufactured in different parts of tunisia, and selectively searched for lactobacillus sakei, a lactic ...200818155310
inactivation of exopolysaccharide and 3-hydroxypropionaldehyde-producing lactic acid bacteria in apple juice and apple cider by enterocin as-48.the bacteriocin enterocin as-48 was tested against exopolysaccharide producing lactic acid bacteria (lab) strains of lactobacillus collinoides, lactobacillus dioliovorans and pediococcus parvulus as well as two 3-hydroxypropionaldehyde (3-hpa)-producing lb. collinoides strains causing apple cider spoilage. in fresh-made apple juice, a bacteriocin concentration of 2.5 microg/ml reduced the lab viable cell counts below detection levels during the course of incubation at 10 and 22 degrees c for mos ...200818164531
population diversity of yeasts and lactic acid bacteria in pig feed fermented with whey, wet wheat distillers' grains, or water at different temperatures.the diversity of populations of yeast and lactic acid bacteria (lab) in pig feeds fermented at 10, 15, or 20 degrees c was characterized by rrna gene sequencing of isolates. the feeds consisted of a cereal grain mix blended with wet wheat distillers' grains (wwdg feed), whey (w feed), or tap water (wat feed). fermentation proceeded for 5 days without disturbance, followed by 14 days of daily simulated feed outtakes, in which 80% of the contents were replaced with fresh feed mixtures. in wwdg fee ...200818223110
diversity of lactic acid bacteria in fermented brines used to make stinky tofu.stinky tofu is a kind of fermented tofu with a strong odor. although stinky tofu is a very popular snack in the asian region, the community of microbes, and especially lactic acid bacteria (lab), indigenous to the fermented brine from which it is made remains poorly described. we examined 168 isolates obtained from the original fermented brine (brine a) and two brines in which the hard tofu (brine b) and soft tofu (brine c) had been soaked. through random amplified polymorphic dna (rapd) analysi ...200818234387
capacity of human nisin- and pediocin-producing lactic acid bacteria to reduce intestinal colonization by vancomycin-resistant enterococci.this study demonstrated the capacity of bacteriocin-producing lactic acid bacteria (lab) to reduce intestinal colonization by vancomycin-resistant enterococci (vre) in a mouse model. lactococcus lactis mm19 and pediococcus acidilactici mm33 are bacteriocin producers isolated from human feces. the bacteriocin secreted by p. acidilactici is identical to pediocin pa-1/ach, while pcr analysis demonstrated that l. lactis harbors the nisin z gene. lab were acid and bile tolerant when assayed under sim ...200818245231
use of response surface method for the determination of demineralization efficiency in fermented shrimp shells.a box-behnken design with three variables (sucrose concentration, initial ph value and soaking time) and three levels were used for studying the demineralization efficiency in fermented shrimp shells by pediococcus sp. l1/2. first, the bacterial cells were inoculated into the media with various concentrations of sucrose and initial ph values, and fermentation took place under static conditions at 37 degrees c for 24h. significant differences in the levels of total titratable acid were observed. ...200818276133
effects of orally administered probiotic pediococcus acidilactici on the small and large intestine of weaning piglets. a qualitative and quantitative micro-anatomical study.probiotic research has been approached, above all in recent years, by widely differing points of view, both for human and animal uses. lactic acid bacteria release bacteriocins, and some of them may function as probiotic. the aim of the present study was to investigate the effects of dietary supplementation with the probiotic pediococcus acidilactici on the piglet intestine, on circulating lymphocytes, and on aspects of piglet performance during the first 42 days after weaning. sixteen female pi ...200818366003
effects of chinese wolfberry (lycium chinense p. mill.) leaf hydrolysates on the growth of pediococcus acidilactici.growth stimulating effects of lych leaf hydrolysates on pediococcus acidilactici imt101 cells were observed when mrs broth was supplemented with 20% (v/v) h1+h2, the mixture of hydrolysates prepared by a traditional tea-making process. cells grown on mrs containing h1+h2 showed a shortened lag phase while yielding a cell concentration (x(s)) significantly higher than other conditions investigated entering stationary phase. the maximal specific growth rate (mu(max)) was also the highest among all ...200817383176
inhibition of escherichia coli o157:h7 in ripening dry fermented sausage by ground yellow mustard.compounds generated by the enzymatic hydrolysis of glucosinolates naturally present in mustard powder are potently bactericidal against escherichia coli o157:h7. because e. coli o157:h7 can survive the dry fermented sausage manufacturing process, 2, 4, and 6% (wt/wt) nondeheated (hot) mustard powder or 6% (wt/wt) deheated (cold) mustard powder were added to dry sausage batter inoculated with e. coli o157:h7 at about 7 log cfu/g to evaluate the antimicrobial effectiveness of the powders. reductio ...200818389690
bartonella quintana and bartonella pediococcus infection after aortic valve replacement.bartonella quintana and bartonella pediococcus infections are very rare causes of endocarditis. urban trench fever with relapsing febrile illness, headache, leg pain, and endocarditis has now begun to be a more important cause of disease in socially disadvantaged persons. the diagnosis is difficult because the growth of b. quintana in blood culture takes 20-40 days. b. pediococcus may be an opportunistic pathogen in severely compromised hosts, although it has been described as a harmless bacteri ...200818430664
transport of glycerol by pediococcus pentosaceus isolated from wine.pediococcus pentosaceus n(5)p is a strain isolated from wine that uses glycerol as its sole carbon source, mainly via the glycerol kinase pathway. the transport of glycerol was investigated in resting cells of this strain. glycerol uptake followed a michaelis-menten relationship with an observed apparent k(m) of 33 microm and a v(max) of 2.5 nmol/min/mg of cell protein. the transport system was specific for glycerol, which was present in the cells grown either on glycerol or glucose suggesting i ...200818456108
multi-loci sequence typing (mlst) for two lacto-acid bacteria (lab) species: pediococcus parvulus and p. damnosus.the control of wine microbial population during and beyond fermentation is of huge importance for wine quality. lactic acid bacteria (lab) in wine are responsible for malolactic fermentation (mlf) which can be desired in some cases and undesirable in others. some lab do not perform mlf and their uncontrolled growth could contribute to severe wine spoilage such as undesired flavours. their identification and detection is considered crucial for numerous biotechnological applications in food fermen ...200818543129
ecological removal of recalcitrant phenolic compounds of treated olive mill wastewater by pediococcus pentosaceus.treatment of olive mill wastewater by different biological process led to an important decrease of organic compounds but the black coloration due to the recalcitrant phenolic compounds persists. experimental design using fractional factorial plan showed that, among the seven studied factors, yeast extract, dilution and glucose exhibited a positive effect on pediococcus pentosaceus growth and treated olive mill wastewater (tomw) decolourisation. optimization of influent factors showed that 2.5 an ...200817686628
pediocin pa-1 production during repeated-cycle batch culture of immobilized pediococcus acidilactici ul5 cells.pediocin pa-1 production by pediococcus acidilactici ul5 cells immobilized in kappa-carrageenan/locust bean gum gel beads was studied during repeated-cycle batch (rcb) culture with ph control in man rogosa and sharpe (mrs) broth supplemented with 1% glucose and whey permeate (swp) medium. the pediocin pa-1 production by free p. acidilactici cells ph-controlled batch culture has reached 2048 and 4096 au ml(-1) after 11 and 12 h of incubation, with volumetric productivities of 187 and 342 au ml(-1 ...200818558343
immunity gene pedb enhances production of pediocin pa-1 in naturally resistant lactococcus lactis strains.heterologous production of the antilisterial bacteriocin pediocin pa-1 in lactococci is an attractive objective to increase the safety of dairy products. in a previous paper, we developed a system for the heterologous production of the bacteriocin pediocin pa-1 in pediocin-resistant lactococcal hosts through a leader exchange strategy. the system was based on 3 genes, 1 encoding the fusion between the lactococcin a leader and propediocin pa-1, and the other 2 encoding the lactococcin a secretion ...200818565917
heterologous expression of a position 2-substituted (1-->3)-beta-d-glucan in lactococcus lactis.exopolysaccharides play an important role in the rheology and texture of fermented foods, and among these beta-glucans have immunomodulating properties. we show that the overproduction of the pediococcus parvulus gtf glycosyltransferase in an uncapsulated lactococcus lactis strain results in synthesis and secretion (300 mg liter(-1)) of a position 2-substituted (1-->3)-beta-d-glucan that has potential use as a food additive.200818567684
characterization of the gut bacterial community in manduca sexta and effect of antibiotics on bacterial diversity and nematode reproduction.the tobacco hornworm, manduca sexta, is a model lepidopteran insect used to study the pathogenic and mutualistic phases of entomopathogenic nematodes (epns) and their bacterial symbionts. while intestinal microbial communities could potentially compete with the epn and its bacterial partner for nutrient resources of the insect, the microbial gut community had not been characterized previously. here, we show that the midgut of m. sexta raised on an artificial diet contained mostly gram-positive c ...200818647359
biochemical characterisation of the esterase activities of wine lactic acid bacteria.esters are an important group of volatile compounds that can contribute to wine flavour. wine lactic acid bacteria (lab) have been shown to produce esterases capable of hydrolysing ester substrates. this study aims to characterise the esterase activities of nine lab strains under important wine conditions, namely, acidic conditions, low temperature (to 10 degrees c) and in the presence of ethanol (2-18% v/v). esterase substrate specificity was also examined using seven different ester substrates ...200717828602
identification of subdominant sourdough lactic acid bacteria and their evolution during laboratory-scale fermentations.presumptive lactic acid bacterial cocci were found in six sourdoughs (out of 20) from the abruzzo region (central italy) and subjected to phenotypic and genotypic characterization. a total of 21 isolates, recognized as seven strains by randomly amplified polymorphic dna (rapd)-polymerase chain reaction (pcr) typing, were identified by a polyphasic approach, consisting of 16s rrna gene sequencing, multiplex pcr assays and physiological features, as enterococcus faecium and pediococcus pentosaceus ...200717418310
identification of lactic acid bacteria from fermented tea leaves (miang) in thailand and proposals of lactobacillus thailandensis sp. nov., lactobacillus camelliae sp. nov., and pediococcus siamensis sp. nov.eighteen rod-shaped homofermentatives, six heterofermentatives, and a coccal homofermentative lactic acid bacteria were isolated from fermented tea leaves (miang) produced in the northern part of thailand. the isolates were placed in a monophyletic cluster consisting of lactobacillus and pediococcus species. they were divided into seven groups by phenotypic and chemotaxonomic characteristics, dna-dna similarity, and 16s rrna gene sequences. groups i to vi belonged to lactobacillus and group vii ...200717429157
development of thermal surrogate microorganisms in ground beef for in-plant critical control point validation studies.in search of a suitable surrogate microorganism for in-plant critical control point validation, we compared the rates of thermal inactivation of three bacteria, enterococcus faecium b2354, pediococcus parvulus hp, and pediococcus acidilactici lp, to those of listeria monocytogenes and salmonella. ground beef samples containing 4 and 12% fat were inoculated with e. faecium, l. monocytogenes, and salmonella senftenberg 775w and heated at 58, 62, 65, or 68 degrees c. the decimal reduction times (d- ...200717477266
development of innovative pediocin pa-1 by dna shuffling among class iia bacteriocins.pediocin pa-1 is a member of the class iia bacteriocins, which show antimicrobial effects against lactic acid bacteria. to develop an improved version of pediocin pa-1, reciprocal chimeras between pediocin pa-1 and enterocin a, another class iia bacteriocin, were constructed. chimera ep, which consisted of the c-terminal half of pediocin pa-1 fused to the n-terminal half of enterocin a, showed increased activity against a strain of leuconostoc lactis isolated from a sour-spoiled dairy product. t ...200717601819
simultaneous microchip enzymatic measurements of blood lactate and glucose.a miniaturized capillary electrophoretic (ce) microchip device for the simultaneous measurements of lactate and glucose is described. the new microchip bioassay protocol integrates an electrophoretic separation of lactate and glucose, post-column enzymatic reactions of these metabolites with their respective oxidase enzymes, and an amperometric (anodic) detection of enzymatically-liberated hydrogen peroxide at a gold-coated thick-film carbon detector. factors influencing the response have been e ...200717386641
lactobacillus composti sp. nov., a lactic acid bacterium isolated from a compost of distilled shochu residue.two strains of lactic acid bacteria, strains nric 0689(t) and nric 0690, were isolated from a compost of distilled shochu residue in japan. the isolates showed quite low sequence similarity to known species of lactic acid bacteria on the basis of 16s rrna gene sequence; the highest sequence similarities to nric 0689(t) were shown by the type strains of lactobacillus satsumensis, l. plantarum, l. hilgardii, l. buchneri and l. parabuchneri (92.9, 92.9, 92.8, 92.6 and 92.5 %, respectively). the iso ...200717392222
quantitative analysis of the intestinal bacterial community in one- to three-week-old commercially reared broiler chickens fed conventional or antibiotic-free vegetable-based diets.to explore the effect of drug-free poultry production on the intestinal microflora of broiler chickens, the bacterial community of this environment was quantitatively profiled in both conventionally reared birds and birds reared without antibiotic growth promotants (agps) on a vegetable-based diet.200717381758
species diversity and relative abundance of vaginal lactic acid bacteria from women in uganda and korea.lactobacilli play an important role in maintaining vaginal health of women. the aim of this study was to compare the species richness and relative abundance of lactobacillus and other lactic acid bacteria in women of two geographically distant countries, uganda and korea.200717381754
antimicrobial activity of nisin against oenococcus oeni and other wine bacteria.nisin is a bacteriocin used against food spoilage bacteria. sulphur dioxide is a potent antioxidant as well as an antimicrobial agent widely used in the wine industry. in this study we describe the effect of these important antibacterial agents on the growth of a collection of 64 lactic acid bacteria (23 oenococcus, 29 lactobacillus, 3 leuconostoc and 9 pediococcus), 23 acetic acid bacteria and 20 yeast isolates, most of them recovered from wine. minimal inhibitory concentrations (mic) and minim ...200717320991
coculture-inducible bacteriocin activity of lactobacillus plantarum strain j23 isolated from grape must.detection and characterization of bacteriocin production by lactobacillus plantarum strain j23, recovered from a grape must sample in spain, have been carried out. bacteriocin activity was degraded by proteolytic enzymes (trypsin, alfa-chymotrypsin, papaine, protease, proteinase k and acid proteases), and it was stable at high temperatures (121 degrees c, 20min), in a wide range of ph (1-12), and after treatment with organic solvents. l. plantarum j23 showed antimicrobial activity against oenoco ...200717367681
characterization of two bacteriocins produced by pediococcus acidilactici isolated from "alheira", a fermented sausage traditionally produced in portugal.lactic acid bacteria were isolated from "alheira" sausages that have been sampled from different regions in portugal. the sausages were produced according to different recipes and with traditional starter cultures. two isolates (ha-6111-2 and ha-5692-3) from different sausages were identified as strains of pediococcus acidilactici. each strain produces a bacteriocin, designated as bacha-6111-2 and bacha-5692-3. both bacteriocins are produced at low levels after 18 h of growth in mrs broth (3200 ...200717368595
influence of the carbohydrate source on beta-glucan production and enzyme activities involved in sugar metabolism in pediococcus parvulus 2.6.the influence of carbohydrate source on growth, exopolysaccharide (eps) production and on the activity of the enzymes implicated in energy generation and udp-glucose synthesis in pediococcus parvulus 2.6 was evaluated. the highest eps production was obtained on glucose, while fructose was a poor substrate for eps synthesis. hplc and nmr analysis on monomer composition and structure of the eps showed that this strain produced the same beta-glucan, regardless of the carbohydrate source. the alpha- ...200717303279
chimeras of mature pediocin pa-1 fused to the signal peptide of enterocin p permits the cloning, production, and expression of pediocin pa-1 in lactococcus lactis.chimeras of pediocin pa-1 (peda-1), a bacteriocin produced by pediococcus acidilactici plbh9, fused to the signal peptide of enterocin p (entp), a sec-dependent bacteriocin produced by enterococcus faecium p13, permitted the production of peda-1 in lactococcus lactis. chimeric genes encoding the entp signal peptide (sp(entp)) fused to mature peda-1 (peda), with or without its immunity gene (pedb), were cloned into the expression vector pmg36c to generate the recombinant plasmids pmpp9 (sp(entp): ...200718095432
[exopolysaccharides of lactic acid bacteria: structure and biosynthesis].the group of lactic acid bacteria (labs) includes four genera: lactobacillus, leuconostoc, pediococcus, and streptococcus. the most characteristic feature of this group of microorganisms is the production of lactic acid as a main product of carbohydrate metabolism. labs are responsible for the fermentation of alimentary products and they also produce a variety of agents, among them exopolysaccharides (epss), which inhibit the growth of pathogenic bacteria. in this article on the different types ...200718097339
rna-based sandwich hybridisation method for detection of lactic acid bacteria in brewery samples.recently we showed the applicability and sensitivity of the rna-based sandwich hybridisation assay (sha) for detection of gram-negative cells in environmental samples [leskelä, t., tilsala-timisjärvi, a., kusnetsov, j., neubauer, p., breitenstein, a., 2005. sensitive genus-specific detection of legionella by a 16s rrna based sandwich hybridization assay. j. microbiol. met. 62, 167-179.]. in this study the aim was to test and optimise this method for the detection of gram-positive cells from brew ...200717157400
characterization and selection of autochthonous lactic acid bacteria isolated from traditional iberian dry-fermented salchichón and chorizo sausages.a total of 192 lactic acid bacteria were isolated from 2 types of naturally fermented dry sausages at 3 different stages of the ripening process in order to select the most suitable strains as starter cultures in dry-cured sausage manufacture according to their technological characteristics such as glucose fermentation, lactic and acetic acid production, and proteolytic, lipolytic, and antimicrobial activities. identification of the isolates revealed that 31.2% were pediococcus pentosaceus, 26.9 ...200717995686
microbial development in distillers wet grains produced during fuel ethanol production from corn (zea mays).distillers grains are coproduced with ethanol and carbon dioxide during the production of fuel ethanol from the dry milling and fermentation of corn grain, yet there is little basic microbiological information on these materials. we undertook a replicated field study of the microbiology of distillers wet grains (dwg) over a 9 day period following their production at an industrial fuel ethanol plant. freshly produced dwg had a ph of about 4.4, a moisture content of about 53.5% (wet mass basis), a ...200718026225
optimization of technical conditions for the transformation of pediococcus acidilactici p60 by electroporation.previously reported techniques for the electrotransfer of foreign dna into pediococci yield only a small number of transformants/mug dna, especially when using undomesticated strains. this study reports an improved protocol for the electrotransformation of pediococci, based on trials using pediococcus acidilactici p60 and the plasmid prs4c1. the improved protocol yields from 2 to 3 log units more transformants than the previously reported methods, with up to (9.1+/-1.3)x10(4) transformants/mug o ...200717275085
identification and antimicrobial susceptibility of lactic acid bacteria from retail fermented foods.one important safety criterion of using lactic acid bacteria (lab) in food applications is to ensure that they do not carry transferable antimicrobial resistance (ar) determinants. in this study, 63 lab belonging to six genera, streptococcus, lactobacillus, lactococcus, enterococcus, leuconostoc, and pediococcus, were recovered from 28 retail fermented food products in maryland, identified to species with 16s-23s rrna spacer pcrs, and characterized for antimicrobial susceptibility against eight ...200718044442
screening and selection of lactic acid bacteria strains suitable for ensiling grass.lactic acid bacteria (lab) strains shown to have broad-spectrum antimicrobial activity were screened for potential as grass silage inoculants. the strains capable of rapidly lowering the ph of the grass matrix and with low proteolytic activity were assessed in laboratory-scale silos in a grass matrix containing natural microbial flora.200717241337
detection and characterization of a lytic pediococcus bacteriophage from the fermenting cucumber brine.of the twelve lytic bacteriophages recovered from five different fermenting cucumber tanks that were inoculated with pediococcus sp. la0281, a lytic phage, phips05, was characterized in the present study. the plaques were mostly clear and round-shaped on the lawn of starter strain, indicating lytic phage. overall appearance indicated that it belongs to the siphoviridae family or bradley's group b1, with a small isometric head and a flexible noncontractile tail with swollen base plate. the averag ...200718051757
antimicrobial susceptibility of non-enterococcal intrinsic glycopeptide-resistant gram-positive organisms.non-enterococcal gram-positive bacteria that are intrinsically vancomycin-resistant have been infrequently isolated in association with serious infections. however, well-documented infections have lately been reported with increasing frequency. because these organisms may be pathogens, we tested the mics of 19 antimicrobial agents by the agar dilution method for predicting susceptibility. the activity of these antimicrobial agents was assessed against 28 strains (lactobacillus rhamnosus, 6; lact ...200717049799
pretreatment with pro- and synbiotics reduces peritonitis-induced acute lung injury in rats.to study whether enteral pretreatment with a synbiotic composition of lactic acid bacteria and bioactive fibers can reduce peritonitis-induced lung neutrophil infiltration and tissue injury in rats.200717426542
detection of the pediocin gene peda in strains from human faeces by real-time pcr and characterization of pediococcus acidilactici uva1.bacteriocin-producing lactic acid bacteria are commonly used as natural protective cultures. among them, strains of the genus pediococcus are particularly interesting for their ability to produce pediocin, a broad spectrum antimicrobial peptide with a strong antagonistic activity against the food-borne pathogen listeria monocytogenes. furthermore, there is increasing interest in isolating new bacteriocin-producing strains of human intestinal origin that could be developed for probiotic effects a ...200717850651
the biodiversity of predominant lactic acid bacteria in dolo and pito wort for the production of sorghum beer.to quantify and identify the predominant lactic acid bacteria (lab) in dolo and pito wort processing, and to examine their biodiversity at strain level.200717897178
effects of pediococcus- and saccharomyces-based probiotic (mitomax) on coccidiosis in broiler chickens.coccidiosis is the major parasitic disease of poultry. in this study, the role of the commercial probiotic mitomax which contains pediococcus acidilactici and saccharomyces boulardii was evaluated by measuring body weight gain, fecal oocyst shedding, and serum antibody responses as an alternative control method of prophylactic drug against coccidiosis. day-old broiler chicks were fed regular or probiotic diets supplemented with mitomax at 0.01%, 0.1%, or 1.0% of diet, and challenged 2 weeks late ...200717434586
pcr monitoring of lactobacillus and bifidobacterium dynamics in fermentations by piglet intestinal microbiota.a new group-specific primer (lact71r), targeting the 16s-23s rdna intergenic spacer region of lactobacillus, was tested in its specificity to amplify rdna of lactobacilli from piglet intestinal origin by polymerase chain reaction (pcr). lact71r and lab0677f, a lactobacillus group-specific primer targeting the 16s rdna, generated a common amplicon by pcr with dna from lactobacillus and pediococcus reference strains, but not from weissella strains. sequence analysis of clones obtained by pcr ampli ...200717440917
sequence analysis of the mobilizable lactococcal plasmid pgdh442 encoding glutamate dehydrogenase activity.a novel plasmid named pgdh442 had previously been isolated from a plant lactococcus lactis strain. this plasmid encodes two interesting properties with applications in the dairy industry: a glutamate dehydrogenase activity that stimulates amino acid conversion to aroma compounds, and cadmium/zinc resistance that can be used as a selectable marker. moreover, this plasmid can be transferred naturally to other strains, but appears to be incompatible with certain other lactococcal plasmids. during t ...200717464081
inventory and monitoring of wine microbial consortia.the evolution of the wine microbial ecosystem is generally restricted to saccharomyces cerevisiae and oenococcus oeni, which are the two main agents in the transformation of grape must into wine by acting during alcoholic and malolactic fermentation, respectively. but others species like the yeast brettanomyces bruxellensis and certain ropy strains of pediococcus parvulus can spoil the wine. the aim of this study was to address the composition of the system more precisely, identifying other comp ...200717235561
high resolution crystal structure of pedb: a structural basis for the classification of pediocin-like immunity proteins.pediocin-like bacteriocins, ribosomally-synthesized antimicrobial peptides, are generally coexpressed with cognate immunity proteins in order to protect the bacteriocin-producer from its own bacteriocin. as a step for understanding the mode of action of immunity proteins, we determined the crystal structure of pedb, a pediocin-like immunity protein conferring immunity to pediocin pp-1.200717537233
modelling the effect of high pressure on the inactivation kinetics of a pressure-resistant strain of pediococcus damnosus in phosphate buffer and gilt-head seabream (sparus aurata).the aim of this research was to: (i) determine the inactivation pattern of a pressure-resistant strain of pediococcus damnosus by high hydrostatic pressure in phosphate buffer (ph 6.7) and gilt-head seabream using the linear, biphasic and weibull models; and (ii) validate the applicability of the weibull model to predict survival curves at other experimental pressure levels.200717578414
lactic acid bacteria and yeasts associated with gowé production from sorghum in bénin.to identify the dominant micro-organisms involved in the production of gowé, a fermented beverage, and to select the most appropriate species for starter culture development.200717650194
influence of geographical origin and flour type on diversity of lactic acid bacteria in traditional belgian sourdoughs.a culture-based approach was used to investigate the diversity of lactic acid bacteria (lab) in belgian traditional sourdoughs and to assess the influence of flour type, bakery environment, geographical origin, and technological characteristics on the taxonomic composition of these lab communities. for this purpose, a total of 714 lab from 21 sourdoughs sampled at 11 artisan bakeries throughout belgium were subjected to a polyphasic identification approach. the microbial composition of the tradi ...200717675431
a taxonomic survey of lactic acid bacteria isolated from wheat (triticum durum) kernels and non-conventional flours.in order to explore the correspondence between raw material- and mature sourdough-lactic acid bacterial (lab) communities, 59 italian wheat (triticum durum) grain samples, one bran and six non-conventional flour samples were analyzed through a culture-dependent approach. the highest cell count by an agar medium specific for lab was 2.16 log cfu/g. from about 2300 presumptive lab (gram-positive and catalase-negative) colonies collected, a total of 356 isolates were subjected to identification by ...200717683888
susceptibility of pediococcus spp. to antimicrobial agents.to investigate the susceptibility of pediococcus species to antimicrobial agents.200717241343
limitations in the use of drosophila melanogaster as a model host for gram-positive bacterial infection.to examine sensitivities of various drosophila melanogaster strains towards human pathogenic and nonpathogenic gram-positive bacteria.200717257264
a five-strain probiotic combination reduces pathogen shedding and alleviates disease signs in pigs challenged with salmonella enterica serovar typhimurium.salmonella spp. infection is a major cause of gastroenteritis, with many thousands of cases reported in the european union every year. the use of probiotics offers the potential to improve this situation. here, we investigate the effects of oral treatment of pigs with a defined lactic acid bacteria culture mixture on both clinical and microbiological signs of salmonella enterica serovar typhimurium infection. fifteen weaned pigs blocked by sex and weight were administered control milk or a mixtu ...200717261517
nanomechanical properties of globular proteins: lactate oxidase.we report on the study of the nanomechanical properties of a lactate oxidase (lox) monolayer immobilized on gold substrates by atomic force microscopy techniques operating under buffer conditions. topographical contact mode imaging evidenced the protein deformation under the applied tip load. we performed approaching force curves with both stiff and soft cantilevers by imposing maximum loads of 1.6 nn and 400 pn, respectively. we found that the experimental data were well fitted by the hertz mod ...200717261045
influence of pediococcus-based probiotic on coccidiosis in broiler chickens.coccidiosis is the major parasitic disease of poultry and is caused by the apicomplexan parasites eimeria. drugs and live vaccines are the 2 main control measures of the disease; however, due to increasing concerns with prophylactic drug use and the high cost of vaccines, alternative control methods are needed. recent evidence that various dietary and live microbial supplements can influence host immunity against enteric diseases prompted us to investigate the role of a pediococcus-based probiot ...200717179417
vanillin production from simple phenols by wine-associated lactic acid bacteria.the ability of lactic acid bacteria (lab) to metabolize certain phenolic precursors to vanillin was investigated.200717209816
antimicrobial susceptibilities of lactobacillus, pediococcus and lactococcus human isolates and cultures intended for probiotic or nutritional use.to determine mics of 16 antimicrobials representing all major classes for 473 taxonomically well-characterized isolates of lactic acid bacteria (lab) encompassing the genera lactobacillus, pediococcus and lactococcus. to propose tentative epidemiological cut-off (ecoff) values for recognizing intrinsic and acquired antimicrobial resistances in numerically dominant species.200717369278
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