Publications

in vitro activities of ketolide hmr3647, macrolides, and other antibiotics against lactobacillus, leuconostoc, and pediococcus isolates.	testing of susceptibility to 13 antibiotics was performed with 90 isolates of lactobacillus, leuconostoc, and pediococcus. mics at which 90% of the isolates tested were inhibited by hmr3647, erythromycin, and ciprofloxacin were 0.015, 0.125 and 32 microg/ml, respectively. the penicillin mic was > or = 16 microg/ml against 26.2% of the studied lactobacillus sp. isolates and 50% of lactobacillus plantarum. hmr3647 showed excellent activity against these genera.	1999	10582908
detection and characterization of a novel antibacterial substance produced by lactobacillus plantarum st 31 isolated from sourdough.	lactobacillus plantarum st31 isolated from sourdough produced an antimicrobial substance inhibiting other strains of the genera lactobacillus, leuconostoc, pediococcus, streptococcus, bacillus and some foodborne pathogens including staphylococcus aureus. this antimicrobial substance was inactivated by proteolytic enzymes. consequently, it was characterized as a bacteriocin and was designated plantaricin st31. this bacteriocin was stable in the ph range 3-8 and it was not affected by amylolytic e ...	1999	10443536
study of the micro-organisms associated with the fermented bread (khamir) produced from sorghum in gizan region, saudi arabia.	traditional bread (khamir) was made from sorghum flour of two local varieties, bayadh and hamra. the bread was prepared by mixing the sorghum flour with water and spices (onion, garlic, lemon juice and fenugreek) in a 1:0.8 (w/w) ratio and fermented for 24 h at 30 degrees c. two other fermentations were carried out using an inoculum from the previous fermentation. the micro-organisms were isolated from different plates and identified using different characterization systems. both total bacterial ...	1999	10063620
classification and characterization of lactic acid bacteria isolated from the intestines of common carp and freshwater prawns.	fifty-four isolates of lactic acid bacteria were obtained from the intestines of the common carp (cyprinus carpio) and freshwater prawns (macrobrachium rosenbergii) in nakorn-pathom province, thailand. all isolates were gram-positive and catalase-negative cocci that did not produce gas from glucose and formed dl or l(+) lactic acid only. most isolates were able to grow in broth at ph 9.6, in 6.5% nacl (w/v) and 40% (w/v) bile. these isolates were divided into six groups (a-f) by sugar fermentati ...	1999	12501375
[studies on the lactate oxidase producing conditions by edwardsiella tarda].	a bacterium producing lactate oxidase was re-screened from five strains based on previous researches. the lactate oxidase activity was the highest in strain l1 and this stain was chosen as the enzyme source. morphological and physiological studies revealed that the bacterial strain l1 belongs to the edwardsiella tarda biogroup i. this stain is different from the reported strains mycobacterium and pediococcus, which produce lactate oxidase. the enzyme producing conditions were studied in shaking ...	1999	12555418
airborne microflora in quebec dairy farms: lack of effect of bacterial hay preservatives.	pediococcus pentosaceus is a lactic-acid producing bacterium inoculated in hay to prevent hay deterioration. this study sought to verify the effect of this treatment on the barn microenvironment. air samples were obtained from 19 barns using bacterial hay treatment and from 18 control barns with six-stage andersen samplers and all-glass impingers. appropriate culture media were used for the recovery and identification of microorganisms. endotoxins were measured with chromogenic limulus amoebocyt ...	1999	10028620
identification of lactic acid bacteria from chili bo, a malaysian food ingredient.	ninety-two strains of lactic acid bacteria (lab) were isolated from a malaysian food ingredient, chili bo, stored for up to 25 days at 28 degreesc with no benzoic acid (product a) or with 7,000 mg of benzoic acid kg-1 (product b). the strains were divided into eight groups by traditional phenotypic tests. a total of 43 strains were selected for comparison of their sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) whole-cell protein patterns with a sds-page database of lab. iso ...	1999	9925588
the influence of manufacturing and drying conditions on the survival and toxinogenesis of staphylococcus aureus in two spanish dry sausages (chorizo and salchichón).	the effects of formulation, starter culture and fermentation temperature on growth and synthesis of toxin a (sea) and tnase by staphylococcus aureus during fermentation and drying of spanish chorizo were investigated. inhibitory factors able to inhibit sea synthesis in culture media were unable to prevent sea production in chorizo fermented at 20 and 30°c, though a lower temperature and starter culture sp318 (lactobacillus sake, pediococcus pentosaceous and staphylococcus xylosus) decreased stap ...	1999	22062705
the effects of a starter culture on the formation of biogenic amines in turkish soudjoucks.	the effects of starter culture containing lactobacillus sake, pediococcus pentosaceus, staphylococcus carnosus plus staphylococcus xylosus on the formation of biogenic amines during ripening of turkish soudjoucks were investigated. determination of eight different biogenic amines was carried out by reverse-phase high performance liquid chromatography (hplc) using diode array detection. it was found that aerobic plate counts (apc) decreased in the samples with added starter culture. lactic acid b ...	1999	22063199
production of bacteriocin-like metabolites by lactic acid cultures isolated from sucuk samples.	a total of 51 sucuk samples, obtained from different regions of turkey, were examined for lactic acid bacteria (lab) strains producing putative bacteriocin-like metabolites. for detection of antagonistic activity, agar spot and well diffusion assay tests were used. lactobacillus sake lb790, listeria monocytogenes li6, staphylococcus aureus st44 and escherichia coli nrrl b-3704 were used as indicator organisms. strains having antimicrobial activity were also tested against clostridium perfringens ...	2000	22060908
growth and effect of staphylococci and lactic acid bacteria on unsaturated free fatty acids.	the growth and the effects of four species of staphylococci and six lactic acid bacteria (lab) of the genus carnobacterium, lactobacillus and pediococcus on unsaturated free fatty acids were studied. the strains were grown in complex medium supplemented either with oleic, linoleic or linolenic acids. growth was followed and oxidation of the substrates measured by tbars. the strains of staphylococcus xylosus 873, 16, staphylococcus warneri 863 and staphylococcus saprophyticus grew well on all the ...	2000	22063710
screening of commercial meat starter cultures at low ph and in the presence of bile salts for potential probiotic use.	the survival of lactic acid bacterial strains from eight meat starter cultures under conditions similar to those in the gastrointestinal tract was determined. the conditions in stomach were simulated by using ph 1-5 phosphate-buffered saline. the conditions in small intestine were simulated by using mrs broth over a ph range 4-7 and two bile salt concentrations (0.15% and 0.30%). the survival capacity at ph 3 was strain dependent. 0.30% bile salts were critical for screening bile salt tolerant s ...	2000	22061286
enzyme, bacterial inoculant, and formic acid effects on silage composition of orchardgrass and alfalfa.	we evaluated the effects of cellulase (from trichoderma longibrachiatum) application rates on neutral detergent fiber (ndf) concentration and fermentation products of orchardgrass (dactylis glomerata l.) and alfalfa (medicago sativa l.) silages harvested with decreasing dry matter (dm) digestibility. additionally, the impacts of inoculant (lactobacillus plantarum and pediococcus cerevisiae), pectinase (from aspergillus niger), or formic acid on silage composition were studied. forages wilted to ...	2000	10908057
bacteriocin production by lactic acid bacteria isolated from rioja red wines.	forty-two lactic acid bacteria (lab) of the genera lactobacillus (32), leuconostoc (6), pediococcus (3) and lactococcus (1), isolated from rioja red wines, were tested for antimicrobial activity. all these strains, as well as 18 leuconostoc oenos and 19 yeast strains were used as indicators. only nine strains showed antimicrobial activity, and all were of the species lactobacillus plantarum, which constitutes the predominant microflora in rioja red wines after alcoholic fermentation. lact. plant ...	2000	10735242
effect of some nutritional and environmental parameters on the production of diacetyl and on starch consumption by pediococcus pentosaceus and lactobacillus acidophilus in submerged cultures.	three series of 5-day submerged cultures with pediococcus pentosaceus mitj-10 and lactobacillus acidophilus hansen 1748 were carried out in starch-based media, and the effect of cultural factors on the changes of starch, diacetyl and amylase activity determined. in axenic cultures, ped. pentosaceus mitj-10 produced more diacetyl (63.27 mg l(-1)) by adding glucose, yeast extract and caco3 (p < 0.01), at 28 degrees c (p < 0.05); but more starch was consumed (18.4 g l(-1)) in the absence of glucose ...	2000	10735253
identification of pediococci by ribotyping.	pediococci are among the most prevalent microbial contaminants in breweries and they can cause ropiness and the accumulation of high levels of diacetyl in beer. the accurate identification of pediococci is important, because different species do not possess equal spoilage potential. in this study, 18 pediococcus strains, mainly of brewery origin, were first identified using phenotypical characterization (api 50 chl and sds-page profiling), and then ribotyped using a riboprinterr system. six pedi ...	2000	10735994
cloning and expression of a plasmid-linked pediocin determinant trait of pediococcus acidilactici f.	plasmid dna from pediococcus acidilactici f was prepared by lysozyme-mutanolysin method and purified by cesium chloride-ethidium bromide (cscl-etbr) density gradient ultracentrifugation. agarose gel electrophoresis of plasmid dna and plasmid-curing experiments suggested that bacteriocin activity was harboured on a small plasmid of approximately 9.1 kb (kilobasepair) in pediococcus acidilactici f. plasmid encoding bacteriocin production in p. acidilactici f was examined for restriction enzyme cle ...	2000	10746198
lactobacillus farciminis md, a newer strain with potential for bacteriocin and antibiotic assay.	a native isolate lactobacillus farciminis md isolated from fermenting mushroom exhibited a high degree of sensitivity to the majority of the bacteriocins produced by strains of lactobacilli, leuconostoc and pediococci. also, the efficacy of lact. farciminis md as a sensitive strain for antibiotic assay was established against different antibiotics including ampicillin, cefazoline, chloramphenicol and nitrofurantoin at concentrations of 30 microg each, showing an inhibition zone of 30 mm diameter ...	2000	10747250
intake, digestibility, and composition of orchardgrass and alfalfa silages treated with cellulase, inoculant, and formic acid fed to lambs.	the objectives of this study were to determine the effect of a cellulase (from trichoderma longibrachiatum) alone or combined with a bacterial inoculant (lactobacillus plantarum and pediococcus cerevisiae) or formic acid on composition, intake, and digestibility of orchardgrass (dactylis glomerata l.) and alfalfa (medicago sativa l.) silages. orchardgrass and alfalfa were harvested at the early heading stage and at the early bloom stage of maturity and wilted to approximately 22 and 32% dm, resp ...	2000	11063325
use of a single, triplicate arbitrarily primed-pcr procedure for molecular fingerprinting of lactic acid bacteria.	arbitrarily primed (ap)-pcr can be used to generate characteristic dna fingerprint patterns. however, small changes in reaction conditions can cause band irreproducibility. in this study, a single methodology encompassing triplicate reactions, which were intentionally exposed to three different annealing temperatures, enabled bands that were reproducibly generated to be recognized. a single triplicate ap-pcr (tap-pcr) procedure, using an 18-mer primer, was developed and used to fingerprint repre ...	2000	10788406
nucleotide sequence and analysis of plasmid pmd136 from pediococcus pentosaceus fbb61 (atcc43200) involved in pediocin a production.	the complete sequence of the 19515-bp plasmid pmd136 from pediococcus pentosaceus fbb61 (atcc43200) has been determined. this plasmid is involved in pediocin a production, a bacteriocin active against a wide range of gram-positive bacteria. it appears to replicate via a theta mechanism, with structures closely related to those of many lactococcal plasmids. genes homologous to mobilization functions are also present, which are similar in sequence and arrangement to moba, mobb, and mobc of some st ...	2000	10686129
characterization and determination of origin of lactic acid bacteria from a sorghum-based fermented weaning food by analysis of soluble proteins and amplified fragment length polymorphism fingerprinting.	the group that includes the lactic acid bacteria is one of the most diverse groups of bacteria known, and these organisms have been characterized extensively by using different techniques. in this study, 180 lactic acid bacterial strains isolated from sorghum powder (44 strains) and from corresponding fermented (93 strains) and cooked fermented (43 strains) porridge samples that were prepared in 15 households were characterized by using biochemical and physiological methods, as well as by analyz ...	2000	10698775
a novel lantibiotic, nukacin isk-1, of staphylococcus warneri isk-1: cloning of the structural gene and identification of the structure.	staphylococcus warneri isk-1, which we had previously reported as pediococcus sp. isk-1, produces a novel bacteriocin, nukacin isk-1. edman degradation of the chemically reduced nukacin isk-1 produced a sequence of 27 amino acids, 7 of which were unidentified. using single-specific-primer-pcr product as a probe, a 3.6-kb hindiii fragment containing the nukacin isk-1 structural gene (nuka) was cloned and sequenced. the deduced amino acid sequence of nukacin isk-1 had 57 amino acids, including a 3 ...	2000	11193411
enterocin 012, a bacteriocin produced by enterococcus gallinarum isolated from the intestinal tract of ostrich.	enterococcus gallinarum strain 012, isolated from the duodenum of ostrich, produced enterocin 012 which is active against ent. faecalis, lactobacillus acidophilus, lact. sake, listeria innocua, propionibacterium acidipropionici, propionibacterium sp., clostridium perfringens, pseudomonas aeruginosa and salmonella typhimurium. one of the four pathogenic strains of escherichia coli isolated from the intestinal tract of ostrich was inhibited by enterocin 012. no antimicrobial activity was recorded ...	2000	10736005
characterization of a phage resistance plasmid, plks, of silage-making lactobacillus plantarum ngri0101.	phage contamination has resulted in abnormal fermentation in silage. we isolated a phage-resistant strain, lactobacillus plantarum ngri0101 from silage. the strain carried two plasmids, plkl (6.8 kb) and plks (2.0 kb). by curing and retransformation of the plasmids, we clarified that plks has phage resistant activity, characterized as no adsorption inhibition. plks has 2,025 bp and three orfs, orfl23, orf132, and orf918. the predicted amino acid sequence of the orf918 product showed high similar ...	2000	10830488
purification, characterization, and primary structure of a novel cell wall hydrolytic amidase, cwha, from achromobacter lyticus.	a novel bacteriolytic enzyme cwha (cell wall hydrolytic amidase) was purified by ion exchange and gel-filtration chromatographies from a commercial bacteriolytic preparation from achromobacter lyticus. cwha exhibited optimal ph at 8.5 and lysed chcl(3)-treated escherichia coli more efficiently than micrococcus luteus, staphylococcus aureus, enterococcus faecalis, and pediococcus acidilactici. the enzyme was inhibited by 1,10-phenanthroline strongly and by edta to a lesser extent, suggesting that ...	2000	10833271
differentiation of tetragenococcus populations occurring in products and manufacturing processes of puffer fish ovaries fermented with rice-bran.	tetragenococcus strains isolated from the manufacturing process of japanese puffer fish ovaries fermented with rice-bran were characterized and differentiated phenotypically and genotypically. a total of 413 tetragenococcus isolates were evaluated. on the basis of five representative substrates, the isolates were grouped into seven groups. an rflp (restriction fragment length polymorphisms) analysis of the 16s rrna gene of representative strains of major groups revealed that they could be groupe ...	2000	10857547
molecular diversity of lactic acid bacteria from cassava sour starch (colombia).	lactic acid bacteria and more particularly lactobacilli and leuconostoc, are widely found in a wide variety of traditional fermented foods of tropical countries, made with cereals, tubers, meat or fish. these products represent a source of bacterial diversity that cannot be accurately analysed using classical phenotypic and biochemical tests. in the present work, the identification and the molecular diversity of lactic acid bacteria isolated from cassava sour starch fermentation were assessed by ...	2000	10930082
heterologous coproduction of enterocin a and pediocin pa-1 by lactococcus lactis: detection by specific peptide-directed antibodies.	antibodies against enterocin a were obtained by immunization of rabbits with synthetic peptides ph4 and ph5 designed, respectively, on the n- and c-terminal amino acid sequences of enterocin a and conjugated to the carrier protein klh. anti-ph4-klh antibodies not only recognized enterocin a but also pediocin pa-1, enterocin p, and sakacin a, three bacteriocins which share the n-terminal class iia consensus motif (ygngvxc) that is contained in the sequence of the peptide ph4. in contrast, anti-ph ...	2000	10919819
isolation and identification of tetracycline resistant lactic acid bacteria from pre-packed sliced meat products.	in recent years, the food chain has been recognised as one of the main routes for transmission of antibiotic resistant bacteria between the animal and human population. in this regard, the current study aimed to investigate if tetracycline resistant (tetr) lactic acid bacteria (lab) are present in ready-to-eat modified atmosphere packed (map) sliced meat products including fermented dry sausage, cooked chicken breast meat and cooked ham. from population graphs based on doubling tetracycline conc ...	2000	10930081
lactobacillus algidus sp. nov., a psychrophilic lactic acid bacterium isolated from vacuum-packaged refrigerated beef.	lactobacillus algidus sp. nov. is described on the basis of 40 strains isolated as one of the predominant bacteria from five specimens of vacuum-packaged beef collected from different meat shops and stored at 2 degrees c for 3 weeks. these strains were quite uniform in the overall characteristics examined. they are facultatively anaerobic, psychrophilic, gram-positive, non-spore-forming, non-motile, lactic acid-homofermentative rods. the cells occurred singly and in pairs on agar media and in ra ...	2000	10843056
analysis of the genetic determinant for production of the pediocin p of pediococcus pentosaceus pep1.	pediococcus pentosaceus pep1 is a vacuum-packaged turkish sausage isolate which produces a potentially novel bacteriocin of the pediocin (anti-listeria) family of peptides designated as pediocin p. curing experiments and plasmid profile analysis indicated that both bacteriocin immunity and production determinants were linked and encoded by 9.0 mda plasmid, phd1.0. attempts to transform purified plasmid phd1.0 into recipient escherichia coli jm109 cells by electroporation were successful but none ...	2000	10986669
development of molecular rapd marker for the identification of pediococcus acidilactici strains.	a rapd analysis performed using a single primer targeted to the pediocin ach/pa-1 gene was carried out on several p. acidilactici strains and on some related species of lactic acid bacteria. the high degree of genetic variability detected in p. acidilactici strains did not allow the selection of a common rapd fragment that could be chosen as a potential species-specific dna marker. nevertheless a 700 bp fragment, that was found to be peculiar of all potential pediocin producer strains analyzed, ...	2000	11108020
quinupristin-dalfopristin resistance among gram-positive bacteria in taiwan.	to understand quinupristin-dalfopristin resistance among clinical isolates of gram-positive bacteria in taiwan, where this agent is not yet available for clinical use, we evaluated 1,287 nonduplicate isolates recovered from january 1996 to december 1999 for in vitro susceptibility to quinupristin-dalfopristin and other newer antimicrobial agents. all methicillin-susceptible staphylococcus aureus (mssa) isolates were susceptible to quinupristin-dalfopristin. high rates of nonsusceptibility to qui ...	2000	11083643
description of paralactobacillus selangorensis gen. nov., sp. nov., a new lactic acid bacterium isolated from chili bo, a malaysian food ingredient.	paralactobacillus selangorensis gen. nov., sp. nov. is described. this organism, isolated from a malaysian food ingredient called chili bo, is an obligatory homofermentative, rod-shaped lactic acid bacterium. the g+c content is 46.1-46.2+/-0.3 mol%. earlier 16s rrna studies showed that this organism constitutes a new taxon distantly related to the lactobacillus casei-pediococcus group. a phenotypic description that distinguishes paralactobacillus selangorensis from other genera of lactic acid ba ...	2000	10826783
identification and enumeration of oleic acid and linoleic acid hydrating bacteria in the rumen of sheep and cows.	the diversity and population densities of facultative anaerobic bacteria with the capacity to hydrate oleic acid and linoleic acid in the rumen of sheep and dairy cows were determined. the screening of representative colonies, from rumen fluid plated aerobically on a range of agar media, revealed that sheep rumen fluid contained hydration-positive strains of streptococcus, staphylococcus, enterococcus, lactobacillus and pediococcus, whereas cow rumen fluid contained hydration-positive strains of ...	2000	10735997
synergistic lethal combination of nitrite and acid ph on a verotoxin-negative strain of escherichia coli o157.	this study was concerned with the possible consequences of reducing the nitrite concentration of a fermented sausage environment on the survival of the pathogen e. coli o157:h45, a verotoxin-negative relative of e. coli o157:h7. a liquid medium, fm, was constructed with a liquid phase, a(w) and ph similar to fermented sausage. survival of e. coli o157:h45 in fm depended on both ph and nitrite concentration. in trials in which the ph was decreased by growing pediococcus acidilactici in fm, surviv ...	2000	10791753
enterococcal-type glycopeptide resistance genes in non-enterococcal organisms.	although the emergence of vancomycin-resistant enterococci can be attributed, in part, to the increasing use of vancomycin in clinical practice, and glycopeptide use in animal husbandry, the origins of the enterococcal vancomycin resistance genes are not clear. the vancomycin resistance-associated genes in enterococcus gallinarum, enterococcus casseliflavus/flavescens, lactobacillus spp., leuconostoc spp., pediococcus spp., and erysipelothrix rhusiopathiae, are not the source of the high-level v ...	2000	10731599
bacteriocin production by lactobacillus salivarius of animal origin.		2000	11184178
genomic subpopulations within the species pediococcus acidilactici detected by multilocus typing analysis: relationships between pediocin ach/pa-1 producing and non-producing strains.	a high degree of genetic polymorphism among p. acidilactici strains was highlighted by a multilocus typing approach analysing several housekeeping genes and by sampling the whole genome using random amplified polymorphic dna (rapd) fingerprint analysis performed by using a single primer peda gene targeted in low-stringency amplification conditions. restriction fragment length polymorphism of the rpoc, ldhd/l and mle genes, and a modified rapd analysis, permitted the grouping of pediococcus acidi ...	2000	10931907
solvent extraction of bacteriocins from liquid cultures.	a solvent extraction method was developed to concentrate lacidin from the culture of lactobacillus acidophilus osu133. the new method concentrates the bacteriocin at the interface between chloroform and the aqueous culture of the producing bacterium. compared with other extraction procedures, the new method effectively recovers higher bacteriocin yield and results in relatively clean preparations. recovery of lacidin by the chloroform extraction procedure, compared with ammonium sulphate precipi ...	2000	10972727
acetaldehyde metabolism by wine lactic acid bacteria.	acetaldehyde is a volatile flavor compound present in many fermented foods and is important in the production of red and white wines. nine strains of the genera lactobacillus and oenococcus were able to metabolize acetaldehyde in a resting cell system, whereas two pediococcus strains were not. acetic acid and ethanol were produced from its degradation. a lactobacillus and an oenococcus were able to degrade so(2)-bound acetaldehyde, as well. a coincubation of resting cells of saccharomyces bayanu ...	2000	11004399
engineering increased stability in the antimicrobial peptide pediocin pa-1.	pediocin pa-1 is a food grade antimicrobial peptide that has been used as a food preservative. upon storage at 4 degrees c or room temperature, pediocin pa-1 looses activity, and there is a concomitant 16-da increase in the molecular mass. it is shown that the loss of activity follows first-order kinetics and that the instability can be prevented by replacing the single methionine residue (met31) in pediocin pa-1. replacing met by ala, ile, or leu protected the peptide from oxidation and had onl ...	2000	11055926
biochemical and genetic characterization of coagulin, a new antilisterial bacteriocin in the pediocin family of bacteriocins, produced by bacillus coagulans i(4).	a plasmid-linked antimicrobial peptide, named coagulin, produced by bacillus coagulans i(4) has recently been reported (b. hyronimus, c. le marrec and m. c. urdaci, j. appl. microbiol. 85:42-50, 1998). in the present study, the complete, unambiguous primary amino acid sequence of the peptide was obtained by a combination of both n-terminal sequencing of purified peptide and the complete sequence deduced from the structural gene harbored by plasmid i(4). data revealed that this peptide of 44 resi ...	2000	11097892
identification of enterococcus species and phenotypically similar lactococcus and vagococcus species by reverse checkerboard hybridization to chaperonin 60 gene sequences.	data from four recent studies (s. h. goh et al., j. clin. microbiol. 36:2164-2166, 1998; s. h. goh et al., j. clin. microbiol. 34:818-823, 1996; s. h. goh et al., j. clin. microbiol. 35:3116-3121, 1997; a. y. c. kwok et al., int. j. syst. bacteriol. 49:1181-1192, 1999) suggest that an approximately 600-bp region of the chaperonin 60 (cpn60) gene, amplified by pcr with a single pair of degenerate primers, has utility as a potentially universal target for bacterial identification (id). this cpn60 ...	2000	11060051
a rapid pcr based method to distinguish between lactococcus and enterococcus.	phenotypic characterisation of lactococcus and enterococcus species remains unreliable as strains of both genera have been isolated which do not conform to the traditional criteria for separation of these genera. a bank of 131 isolates was phenotypically characterised by three methods: (a) traditional broth tests, (b) api rapid id 32 strep and (c) bbl crystal id kits. differences in genus designation between commercial kits were evident for 12 strains (9%), while 7 strains (5%) remained unidenti ...	2000	11249021
a case of septicemia with pediococcus acidilactici after long-term antibiotic treatment.	presented here is a case of septicemia caused by an uncommon, multiresistant, gram-positive microorganism (pediococcus acidilactici) after long-term antibiotic treatment. pediococcus spp. are rarely cultivated from clinical specimens, and species differentiation is difficult due to the paucity of phenotypic traits. in this case, a polyphasic approach consisting of phenotypic and molecular genetic analyses was used, and the identification of pediococcus acidilactici was conclusive. precise identi ...	2000	11205633
inducible metabolism of phenolic acids in pediococcus pentosaceus is encoded by an autoregulated operon which involves a new class of negative transcriptional regulator.	pediococcus pentosaceus displays a substrate-inducible phenolic acid decarboxylase (pad) activity on p-coumaric acid. based on dna sequence homologies between the three pads previously cloned, a dna probe of the lactobacillus plantarum pdc gene was used to screen a p. pentosaceus genomic library in order to clone the corresponding gene of this bacteria. one clone detected with this probe displayed a low pad activity. subcloning of this plasmid insertion allowed us to determine the part of the in ...	2000	11073918
stimulation of starter culture for further reduction of foodborne pathogens during salami fermentation.	this study was conducted to determine if stimulated meat starter culture (msc; pediococcus acidilactici) would further control escherichia coli o157:h7, listeria monocytogenes, and staphylococcus aureus during salami fermentation. manganese ion (0.005% of mnso4) was used as a stimulator for the growth and acid production of msc. after 24-h salami fermentation, nonstimulated msc and stimulated msc reduced e. coli o157:h7 levels by 1.3 and 2.3 log10 units, respectively. nonstimulated msc reduced l ...	2000	11079689
[molecular structure of the locus for sucrose utilization by lactobacillus plantarum: comparison with pediococcus pentosaceus].	structure of the sucrose utilization locus in a lactobacillus plantarum type strain was studied using pcr and southern hybridization. restriction map analysis revealed its high similarity to the sequenced sucrose utilization locus of pediococcus pentosaceus psrq1. the l. plantarum locus proved containing oppositely oriented scra and the scrbragl operon, but not agas. the l. plantarum sucrase gene (scrb) was partly sequenced. a higher (98.6%) homology was revealed between scrb than between the 16 ...	2001	11234378
optimum production, stability, partial purification and inhibitory spectrum of antimicrobial compounds produced by pediococcus pentosaceus di.	the optimum conditions for production of antimicrobial compounds by pediococcus pentosaceus di were studied. the antimicrobial compounds in cell free fermented medium were extracted (methanol-acetone), then tested for inhibitory activity against fourteen bacterial strains and its stability to heating, various ph and proteolytic enzymes. in addition, methanol-acetone extract was partial purified through sephadex g-100 column and thin-layer chromatography. production of antimicrobial compounds wer ...	2001	11379284
detection of lactobacillus, pediococcus, leuconostoc, and weissella species in human feces by using group-specific pcr primers and denaturing gradient gel electrophoresis.	denaturing gradient gel electrophoresis (dgge) of dna fragments generated by pcr with 16s ribosomal dna-targeted group-specific primers was used to detect lactic acid bacteria (lab) of the genera lactobacillus, pediococcus, leuconostoc, and weissella in human feces. analysis of fecal samples of four subjects revealed individual profiles of dna fragments originating not only from species that have been described as intestinal inhabitants but also from characteristically food-associated bacteria s ...	2001	11375166
luminescent method for the detection of antibacterial activities.	a new rapid and sensitive method for the detection of antibacterial activities was based on luminescent indicator strains. listeria innocua 8811 and enterococcus faecalis 32 were transformed with plasmid carrying bacterial luciferase genes. subsequent strains became capable to emit light during the exponential growth phase. the addition of bacteriocin containing culture supernatants to such cultures induced a drop of their light emission which was correlated to the combined antibacterial activit ...	2001	11778890
enterococcal diversity in the environment of an irish cheddar-type cheesemaking factory.	enterococci are natural residents of human and animal intestinal tracts and grow to high levels in a variety of artisanal cheeses. the aim of this study was to determine the diversity of enterococci in a farmhouse raw-milk cheese production unit. putative enterococci were isolated from the faeces of all the cows and all the people associated with the cheesemaking, from the milk and cheese during manufacture and ripening and from the environment in three separate trials. almost 1400 isolates were ...	2001	11789936
the gram-positive tonsillar and nasal flora of piglets before and after weaning.	to investigate gram-positive nasal and tonsillar microbial flora of piglets before and after weaning.	2001	11851806
flocculation and coflocculation of bacteria by yeasts.	biotransformations in natural environments frequently involve interactions between microorganisms. although there are many reports on the interactions between bacteria, interactions between yeasts and bacteria have not been extensively studied. previously we reported on the flocculation and coflocculation of pediococcus damnosus by saccharomyces cerevisiae. now we report that several other yeasts, such as candida utilis, dekkera bruxellensis, hanseniaspora guilliermondii, kloeckera apiculata, an ...	2001	11525628
[biological deacidification of wines using lactic-acid bacteria and yeasts].	based on a study of 200 lactic-acid bacteria monocultures and 30 associating lactic bacteria and yeasts cultures, a stable association was created formed by leuconostoc oenos, pediococcus pentosaceus and saccharomyces cerevisiae yeasts, intended for the biological deacidification of wine. physiology of microorganisms and their effect on the wine chemical composition was studied. by means of selective association, high quality fine wines were produced from the high-acid wines.	2001	11530675
efficient protoplast regeneration for some homofermentative lactobacilli and pediococci.	conditions for protoplast regeneration were examined for several strains of homofermentative lactobacilli and pediococci isolated from silage. attempts to regenerate protoplasts using previously published agar regeneration media for lactobacilli were unsuccessful for most of the strains. replacing or increasing colloidal substances in a medium containing raffinose and mgcl(2) as osmotic stabilizers enabled efficient regeneration of the protoplasts at a frequency of 10-99%. a medium containing ge ...	2001	11797042
dry sausage fermented by lactobacillus rhamnosus strains.	the ability of three probiotic lactobacillus rhamnosus strains gg, e-97800 and lc-705 and one commercial pediococcus pentosaceus starter strain (control) to produce dry sausage was studied. during the fermentation process the numbers of inoculated lactic acid bacteria increased from approx. 7 log10 to 8-9 log10 cfu/g and the ph values decreased from 5.6 to 4.9-5.0. the sensory test indicated that the dry sausages fermented by l. rhamnosus lc-705 were inferior to the control sausages. the presenc ...	2001	11252505
characterization, production, and purification of carnocin h, a bacteriocin produced by carnobacterium 377.	carnocin h, a bacteriocin produced by a carnobacterium sp., inhibited lactic acid bacteria, clostridia, enterococci, and some staphylococcus aureus strains. some strains of listeria and pediococcus were also sensitive to carnocin h. the bacteriocin was produced during the late stationary growth phase. carnocin h was purified by cation exchange chromatography and reverse phase chromatography. amino acid sequence and composition indicate that carnocin h is a novel bacteriocin belonging to the clas ...	2001	11683354
bifunctional enzyme 6'-n-aminoglycoside acetyltransferase-2"-o-aminoglycoside phosphotransferase in lactobacillus and pediococcus isolates of animal origin.		2001	11281121
phenotypic and genotypic characterization of pediococcus strains isolated from human clinical sources.	seventy-two strains of pediococci isolated from human clinical sources were characterized by conventional physiological tests, chromogenic enzymatic tests, analysis of whole-cell protein profiles (wcpp) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and analysis of chromosomal dna restriction profiles by pulsed-field gel electrophoresis (pfge). conventional tests allowed identification of 67 isolates: 52 strains were identified as pediococcus acidilactici, 15 strains were identifi ...	2001	11283035
direct polymerase chain reaction detection of ropy pediococcus damnosus strains in wine.	glucan-producing strains of pediococcus damnosus are considered as spoilage micro-organisms because synthesis of glucan leads to an unacceptable viscosity of wine. in this report, we present a polymerase chain reaction (pcr) procedure to detect the presence of such strains in wines.	2001	11309064
expression in escherichia coli of native and chimeric phenolic acid decarboxylases with modified enzymatic activities and method for screening recombinant e. coli strains expressing these enzymes.	four bacterial phenolic acid decarboxylases (pad) from lactobacillus plantarum, pediococcus pentosaceus, bacillus subtilis, and bacillus pumilus were expressed in escherichia coli, and their activities on p-coumaric, ferulic, and caffeic acids were compared. although these four enzymes displayed 61% amino acid sequence identity, they exhibit different activities for ferulic and caffeic acid metabolism. to elucidate the domain(s) that determines these differences, chimeric pad proteins were const ...	2001	11229892
behavior of variable v3 region from 16s rdna of lactic acid bacteria in denaturing gradient gel electrophoresis.	separation of amplified v3 region from 16s rdna by denaturing gradient gel electrophoresis (dgge) was tested as a tool for differentiation of lactic acid bacteria commonly isolated from food. variable v3 regions of 21 reference strains and 34 wild strains referred to species belonging to the genera pediococcus, enterococcus, lactococcus, lactobacillus, leuconostoc, weissella, and streptococcus were analyzed. dgge profiles obtained were species-specific for most of the cultures tested. moreover, ...	2001	11270655
flow cytometric assessment of viability of lactic acid bacteria.	the viability of lactic acid bacteria is crucial for their applications as dairy starters and as probiotics. we investigated the usefulness of flow cytometry (fcm) for viability assessment of lactic acid bacteria. the esterase substrate carboxyfluorescein diacetate (cfda) and the dye exclusion dna binding probes propidium iodide (pi) and toto-1 were tested for live/dead discrimination using a lactococcus, a streptococcus, three lactobacillus, two leuconostoc, an enterococcus, and a pediococcus s ...	2001	11319119
bacteremic infection with pediococcus: vancomycin-resistant opportunist.	pediococci are recently recognized gram-positive human pathogens, resistant to vancomycin and generally susceptible to penicillin. infection in adults has been seen in patients with chronic underlying conditions as well as those with previous abdominal surgery. two previous infants with congenital gastrointestinal malformations requiring surgical correction have been reported with sepsis attributable to pediococcus sp. we report a third infant born with gastroschisis who developed pediococcus ba ...	2001	11335758
selection and characterization of mixed starter cultures for lactic acid fermentation of carrot, cabbage, beet and onion vegetable mixtures.	an evaluation of various lactic acid bacteria (lab) for the fermentation of cabbage, carrot and beet-based vegetable products was carried out. as part of a screening process, the growth of 15 cultures in a vegetable juice medium (vjm) was characterized by automated spectrophotometry. acidification patterns as well as viability during storage of the lab were also established. there were greater differences between the pure cultures than the mixed ones with respect to growth in vjm and viability d ...	2001	11294348
decrease in cell surface galactose residues of schizosaccharomyces pombe enhances its coflocculation with pediococcus damnosus.	pediococcus damnosus can coflocculate with saccharomyces cerevisiae and cause beer acidification that may or may not be desired. similar coflocculations occur with other yeasts except for schizosaccharomyces pombe which has galactose-rich cell walls. we compared coflocculation rates of s. pombe wild-type species tp4-1d, having a mannose-to-galactose ratio (man:gal) of 5 to 6 in the cell wall, with its glycosylation mutants gms1-1 (man:gal = 5:1) and gms1delta (man:gal = 1:0). these mutants coflo ...	2001	11472912
riboprinting and 16s rrna gene sequencing for identification of brewery pediococcus isolates.	a total of 46 brewery and 15 atcc pediococcus isolates were ribotyped using a qualicon riboprinter. of these, 41 isolates were identified as pediococcus damnosus using ecori digestion. three atcc reference strains had patterns similar to each other and matched 17 of the brewery isolates. six other brewing isolates were similar to atcc 25249. the other 18 p. damnosus brewery isolates had unique patterns. of the remaining brewing isolates, one was identified as p. parvulus, two were identified as ...	2001	11157216
subtraction hybridization in microplates: an improved method to generate strain-specific pcr primers.	an improved subtraction hybridization technique was developed and evaluated. the hybridization is performed in a microplate with the subtractor-dna immobilized in the plate while the probe-dna is in solution. after hybridization the probe-specific dna can easily be removed from the microwell and submitted to further analysis. this new technique has been successfully applied to generate several strain-specific pcr-primers for lactococcus lactis subsp. lactis, pediococcus spec., saccharomyces spec ...	2001	11403389
lactobacillus coleohominis sp. nov., isolated from human sources.	four strains of a hitherto unrecognized gram-positive, catalase-negative, facultatively anaerobic, rod-shaped bacterium isolated from human sources were characterized using phenotypic and molecular taxonomic methods. comparative 16s rrna gene sequencing demonstrated that the bacterium represents a new subline within the lactobacillus casei/pediococcus rrna group of the genus lactobacillus. the unknown bacterium was readily distinguished from all other described lactobacillus species and related ...	2001	11760950
phenotypic diversity of lactic acid bacteria isolated from fermented sausages produced in basilicata (southern italy).	to evaluate the evolution of lactic acid bacteria (lab) populations in traditional fermented sausages (salsiccia and soppressata) produced in artisanal and industrial plants in basilicata (southern italy).	2001	11412324
effect of physical factors on the production of bacteriocin from pediococcus acidilactici itv 26.	the effect of ph, temperature and agitation on growth and bacteriocin production by pediococcus acidilactici itv 126 was investigated. experiments were made in flasks containing mrs medium at 30 to 40 degrees c, ph 5 to 7 and agitation 0 to 200 rpm. factor levels were arranged in a 2(3) factorial design with central and axial points. anova and tukey paired comparison tests showed that a temperature of 35 degrees c favored bacteriocin production, whereas 40 degrees c was best for cell growth. a s ...	2001	11464266
detection and typing of vancomycin-resistance genes of enterococci from clinical and nosocomial surveillance specimens by multiplex pcr.	ninety-three clinical isolates of vancomycin-resistant enterococci (vre) collected from nine hospitals in taiwan were examined for the presence of vana, vanb, vanc1, or vanc2/vanc3 genes by a multiplex pcr. forty-seven of these vre isolates were vana positive, 1 contained both vanc1 and vana, 40 harboured vanb, 2 were vanc1, and 3 were identified to be vanc2/vanc3. twenty-four vana isolates were sensitive to teicoplanin and thus did not have a typical vana phenotype. five isolates with the vanc ...	2001	11467792
nutritional factors affecting the production of two bacteriocins from lactic acid bacteria on whey.	the ability of lactococcus lactis subsp. lactis cect 539 and pediococcus acidilactici nrrl b-5627 to produce bacteriocins on both diluted and concentrated whey was investigated in batch fermentations. both strains produced the higher amounts of biomass and bacteriocin titres on diluted whey. luedeking and piret expression was able to model the production of nisin, which was produced as a primary metabolite on both culture media. however, the pediocin production could not be typified in any case ...	2001	11764192
optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate pcr.	to reduce pcr bias derived from a primer mismatch, the effect of the annealing temperature on the product ratio was investigated by denaturing gradient gel electrophoresis analysis of pcr products from a mixture of perfect-match and one-mismatch templates. these templates were generated by pcr from pediococcus acidilactici for one mismatch and micrococcus luteus for the perfect match. pcrs showed that the bias was reduced at lower temperatures. an environmental sample was also examined.	2001	11472961
comparison of pattern recognition techniques for the identification of lactic acid bacteria.	the goal of this study was to evaluate three pattern recognition methods for use in the identification of lactic acid bacteria.	2001	11473587
the in vitro activity of daptomycin against 514 gram-positive aerobic clinical isolates.	the in vitro activity of daptomycin was assessed in comparison with that of vancomycin and penicillin against a wide range of gram-positive aerobic clinical isolates. mics were determined by an agar dilution method on mueller-hinton agar (nccls/eucast) and on isotonic agar adjusted to contain 50 mg/l free calcium (bsac). both media were enriched with 5% horse blood for fastidious organisms. daptomycin mics for all 172 staphylococci, including methicillin-susceptible and methicillin-resistant sta ...	2001	11481291
conformational changes in pediocin ach upon vesicle binding and approximation of the membrane-bound structure in detergent micelles.	pediocin ach is a 44-residue antimicrobial peptide with bactericidal potency against gram-positive bacteria such as listeria. it belongs to a family of bacteriocins that, when membrane-associated, is predicted to contain beta-sheet and alpha-helical regions. all bacteriocins in this family have a conserved n-terminal disulfide bond. an additional c-terminal disulfide bond in pediocin ach is thought to confer enhanced potency and broader specificity range against sensitive bacteria. the c-termina ...	2001	11705396
the effect of lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage.	whole-plant barley (39.4% dry matter) was treated with various chemical and biological additives to assess their effects on silage fermentation and aerobic stability. treatments were untreated forage, forage treated with several amounts of lactobacillus buchneri and enzymes (l. buchneri at 1 x 10(5), 5 x 10(5), and 1 x 10(6) cfu/g of fresh forage), forage treated with an inoculant containing (lactobacillus plantarum, pediococcus pentosaceus, propionibacterium freudenreichii, and enzymes), or for ...	2001	11384041
characterisation of is153, an is3-family insertion sequence isolated from lactobacillus sanfranciscensis and its use for strain differentiation.	an insertion sequence has been identified in the genome of lactobacillus sanfranciscensis dsm 20451t as segment of 1351 nucleotides containing 37-bp imperfect terminal inverted repeats. the sequence of this element encodes two out of phase, overlapping open reading frames, orfa and orfb, from which three putative proteins are produced. orfab is a transframe protein produced by -1 translational frame shifting between orf a and orf b that is presumed to be the transposase. the large orfab of this ...	2001	11822682
growth optimization of pediococcus damnosus ncfb 1832 and the influence of ph and nutrients on the production of pediocin pd-1.	optimization of the growth of pediococcus damnosus ncfb 1832 and the production of pediocin pd-1 by traditional fermentation methods.	2001	11851822
effect of starter culture and storage temperature on the content of biogenic amines in dry fermented sausage polican.	water activity, ph, microbial counts (total counts/tcm/, coliforms, enterobacter, proteus, pseudomonas, escherichia and lactobacillus spp., respectively), and seven biogenic amines (ba) were determined in dry fermented sausage 'polican' produced using starter culture a (lactobacillus sakei, staphylococcus carnosus, s. xylosus) or b (l. sakei, s. carnosus, pediococcus pentosaceus), ripened 42 days, and subsequently stored at 8 or 22°c 60 days. counts of lactobacilli were higher and tcm lower in t ...	2001	22062781
flavour profiles of dry sausages fermented by selected novel meat starter cultures.	probiotic or bioprotective lactobacillus rhamnosus strains gg, lc-705 and e-97800 as well as pediococcus pentosaceus e-90390 and lactobacillus plantarum e-98098 were studied for their ability to act as main fermenting organisms in the manufacturing process of dry sausages. in the preliminary tests, their abilities to produce lactic acid and biogenic amines, histamine or tyramine, were studied in mrs broth and analysed by high-performance liquid chromatography. the strains produced higher or equa ...	2001	22062105
bacteriocinogenic activity from starter cultures used in spanish meat industry.	thirty-nine bacterial strains, obtained from commercial starter cultures and commonly used by the meat industry in spain, have been examined for their ability to produce bacteriocins. fourteen (35%) of them showed antagonism against at least one of the indicator strains, by the agar spot test. the strains showing an inhibitory action against pathogenic indicator strains were identified as pediococcus acidilactici, lactobacillus curvatus, lactobacillus pentosus and lactobacillus plantarum, which ...	2002	22061417
fermentation and microflora of plaa-som, a thai fermented fish product prepared with different salt concentrations.	plaa-som is a thai fermented fish product prepared from snakehead fish, salt, palm syrup and sometimes roasted rice. we studied the effects of different salt concentrations on decrease in ph and on microflora composition during fermentation. two low-salt batches were prepared, containing 6% and 7% salt (w/w) as well as two high-salt batches, containing 9% and 11% salt. ph decreased rapidly from 6 to 4.5 in low-salt batches, whereas in high-salt batches, a slow or no decrease in ph was found. lac ...	2002	11883675
pediocin pa-1, a wide-spectrum bacteriocin from lactic acid bacteria.	pediocin pa-1 is a broad-spectrum lactic acid bacteria bacteriocin that shows a particularly strong activity against listeria monocytogenes, a foodborne pathogen of special concern among the food industries. this antimicrobial peptide is the most extensively studied class ila (or pediocin family) bacteriocin, and it has been sufficiently well characterized to be used as a food biopreservative. this review focuses on the progress that have been made in the elucidation of its structure, mode of ac ...	2002	11934133
growth and aroma production by staphylococcus xylosus, s. carnosus and s. equorum--a comparative study in model systems.	a laboratory medium inoculated with 20 different staphylococcus strains was prepared in accordance with a full factorial experimental design investigating the effect of temperature, ph, nacl and glucose on growth. the 12 strains most suited to growth in a fermented meat environment were inoculated in sausage minces together with pediococcus pentosaceus, incubated at 25 degrees c for 1 week and the produced aroma compounds collected. the data were analysed by multiple linear regression and partia ...	2002	11999121
factors affecting the adsorption of buchnericin lb, a bacteriocin produced by lactobacillus [correction of lactocobacillus] buchneri.	buchnericin-lb adsorbs to gram-positive but not to gram-negative bacteria. the tested gram-positive bacteria were species of lactobacillus, pediococcus, leuconostoc, enterococcus, lactococcus, listeria, bacillus, staphylococcus; gram-negative bacteria belonged to the genera salmonella, escherichia, yersinia and pseudomonas. buchnericin-lb adsorption depended on ph but not on time and temperature. also some anions of salts and lipoteichoic acid reduced or inhibited its adsorption. treatment of ce ...	2002	12002398
monitoring the identity of bacteria using their intrinsic fluorescence.	three different fluorescence spectra were recorded following excitation at 250 nm (aromatic amino acids+nucleic acids), 270 nm (tryptophan residues) and 316 nm (nadh) for 25 strains of bacteria in dilute suspensions. evaluation of the spectra using principal component analysis and hierarchical clustering showed a good reproducibility from culture to culture and a good discrimination of the bacteria. applying the method of mahalanobis distances to the spectra of lactobacilli species recorded foll ...	2002	12076805
the lactic acid bacteria: a literature survey.	the purpose of this review article on the lactic acid bacteria grew from an early curiosity and a desire to convey and impart the broad scope of literary information on their functions as starter cultures, in the manufacture of fermentation products such as dairy products and alcoholic beverages, as well as their contribution to better health. this review article is an attempt to empower the reader and to circumvent the difficult task in acquiring and elucidating a large body of information. the ...	2002	12546196
the evaluation of mixtures of yeast and potato extracts in growth media for biomass production of lactic cultures.	the effectiveness of yeast extracts (ye) and potato extracts (pe) to promote growth of seven lactic cultures was evaluated by automated spectrophotometry (as). two aspects of the growth curve were analysed: (1) maximum biomass obtained (using odmax) and (2) highest specific growth rate mu(max)) eleven lots from the same pe-manufacturing process were examined for lot-to-lot variability. the odmax values of three of the seven strains were significantly affected by lot source, but mu(max) was not s ...	2002	12224561
multilocus hybridization typing in pediococcus acidilactici strains.	in previous a study we demonstrated the presence of several genomic subpopulations within a collection of pediococcus acidilactici strains isolated from different environments, through a multilocus typing analysis taking into consideration housekeeping conserved loci and protein coding genes of the primary metabolism. in this study, representative strains of five genomic subpopulations previously described (i, ii, iii, v, vii) were analyzed by restriction analysis of chromosomal dna and subseque ...	2002	11815849
genomic diversity within the genus pediococcus as revealed by randomly amplified polymorphic dna pcr and pulsed-field gel electrophoresis.	the genomic diversity of 33 previously assigned strains from six species within the genus pediococcus was assessed by randomly amplified polymorphic dna (rapd) pcr and pulsed-field-gel electrophoresis (pfge). the rapd pcr patterns produced by two separate random primers, termed p1 (acgcgccct) and p2 (atgtaacgcc), were compared by the pearson correlation coefficient and the unweighted pair group method with arithmetic averages clustering algorithm. pattern variations between repeat samples set a ...	2002	11823217
competitive inhibition of listeria monocytogenes in ready-to-eat meat products by lactic acid bacteria.	forty-nine strains of lactic acid bacteria (lab), isolated from commercially available ready-to-eat (rte) meat products, were screened for their ability to inhibit the growth of listeria monocytogenes at refrigeration (5 degrees c) temperatures on agar spot tests. the three most inhibitory strains were identified as pediococcus acidilactici, lactobacillus casei, and lactobacillus paracasei by 16s rdna sequence analysis. their antilisterial activity was quantified in associative cultures in deman ...	2002	11848562
identification of the bacterial microflora in dairy products by temporal temperature gradient gel electrophoresis.	numerous microorganisms, including bacteria, yeasts, and molds, are present in cheeses, forming a complex ecosystem. among these organisms, bacteria are responsible for most of the physicochemical and aromatic transformations that are intrinsic to the cheesemaking process. identification of the bacteria that constitute the cheese ecosystem is essential for understanding their individual contributions to cheese production. we used temporal temperature gradient gel electrophoresis (ttge) to identi ...	2002	12147461
apparent antifungal activity of several lactic acid bacteria against penicillium discolor is due to acetic acid in the medium.	fifty-six dairy bacteria belonging to the genera lactococcus, lactobacillus, pediococcus, propionibacterium, streptococcus, enterococcus, leuconostoc, and brevibacterium were screened for antifungal activity against four species of fungi relevant to the cheese industry (penicillium discolor, penicillium commune, penicillium roqueforti, and aspergillus vesicolor). most of the active strains belonged to the genus lactobacillus, whereas penicillium discolor was found to be the most sensitive of the ...	2002	12182485
production of bacteriocins from lactococcus lactis subsp. lactis cect 539 and pediococcus acidilactici nrrl b-5627 using mussel-processing wastes.	the growth and bacteriocin production by lactococcus lactis subsp. lactis cect 539 and pediococcus acidilactici nrrl b-5627 were investigated on mussel-processing wastes. both bacteriocin productions were satisfactorily modelled using a modified form of the luedeking and piret expression, which includes a term for the influence of the ph reduction rate. experimental data from cultures buffered at different initial concentrations (0, 0.03, 0.10 and 0.25 m) of both bacteria were used to fit and ve ...	2002	12241553
a pcr-based method for identification of lactobacilli at the genus level.	we developed a polymerase chain reaction (pcr)-based method for the identification of lactobacilli at the genus level. one specific primer, lblma1-rev, was designed by analysing similarities between the nucleotide sequence of the spacer between the 16s and 23s rrna genes in a number of lactobacillus strains. amplification with lblma1-rev and r16-1, a universal primer, generated a pcr product for 23 lactobacillus species. electrophoresis did not reveal any discrete bands when escherichia coli, la ...	2002	12351242