Publications

isolation and characterization of is1165, an insertion sequence of leuconostoc mesenteroides subsp. cremoris and other lactic acid bacteria.	we have cloned and characterized an insertion sequence from leuconostoc mesenteroides subsp. cremoris strain db1165. this element, designated is1165, is 1553 bp, has imperfect inverted repeat ends, contains an open reading frame of 1236 bp, and is not related to any previously described insertion sequence. the copy number of is1165 varies from 4 to 13 in l. mesenteroides subsp. cremoris strains allowing genetic fingerprinting of strains based on location and number of bands on hybridization. is1 ...	1992	1325060
reaction mechanism of glutamate racemase, a pyridoxal phosphate-independent amino acid racemase.	glutamate racemase of pediococcus pentosaceus contained no cofactor, and was completely inactivated by a thiol reagent. the role of a cysteine residue in the enzyme reaction was studied by chemical modification. the modification of this cysteine residue resulted in a concomitant loss of activity. dl-glutamate protected the enzyme from inactivation. the inactivated enzyme was reactivated by addition of dithiothreitol. the racemization in 2h2o showed an overshoot in the optical rotation of glutama ...	1992	1358877
nucleotide and amino acid sequence of pap-gene (pediocin ach production) in pediococcus acidilactici h.	n-terminal analysis of purified pediocin ach produced a partial sequence of 23 amino acids. this sequence matched perfectly with a segment of 23 amino acids in a 62 amino acid molecule generated from the 186 nucleotide sequence open reading frame in a hind iii fragment in psmb74 encoding pap-gene (pediocin ach production). it is suggested that the molecule is translated as inactive prepediocin ach of 62 amino acids. then through enzymatic modifications the leader segment of 18 amino acids is rem ...	1992	1368421
characterization and antibiotic susceptibility of pediococcus acidilactici strains isolated from neutropenic patients.		1992	1396762
septicemia and hepatic abscess caused by pediococcus acidilactici.	a case of postoperative pediococcus acidilactici septicemia with parallel isolation of the organism from hepatic specimens is presented. laboratory methods to identify this vancomycin-resistant gram-positive cocci are described. very few cases of documented infections due to this bacterium have been reported in the literature.	1992	1396771
purification and amino acid sequence of a bacteriocin produced by pediococcus acidilactici.	a bacteriocin produced by pediococcus acidilactici has been purified to homogeneity by a rapid and simple four-step purification procedure which includes ammonium sulphate precipitation, chromatography with a cation-exchanger and octyl sepharose, and reverse-phase chromatography. the purification resulted in an approximately 80,000-fold increase in the specific activity and about a 6-fold increase in the total activity. the amino acid composition and sequencing data indicated that the bacterioci ...	1992	1402795
glucose and sucrose fermenting capacity of homofermentative lactic acid bacteria used as starters in fermented salads.	salads, i.e., vegetables and/or meat in an oil-in-water emulsion with a low ph due to the addition of acids, are popular in western countries. the shelf life of these salads is influenced mainly by storage temperature and the use of preservatives, i.e., sorbic and benzoic acid. lactic acid fermentation could be an interesting novel approach to salad preparation. as part of an investigation on fermented salads, lactic acid bacteria from the genera lactobacillus, pediococcus and streptococcus were ...	1992	1419542
in vitro activity of mersacidin (m87-1551), an investigational peptide antibiotic tested against gram-positive bloodstream isolates.	we measured the in vitro activity of mersacidin (formerly m87-1551) against 183 clinical isolates (vancomycin susceptible) and 12 additional vancomycin-resistant strains of gram-positive bacteria. the activity for mersacidin increased an average twofold (range, 1.7- to 7.6-fold) in a calcium-enriched medium. the minimum inhibitory concentration (mic)90 for mersacidin was 8-32 times higher than vancomycin for staphylococci, 4-64 times higher for enterococci, and up to 32 times higher for other or ...	1992	1424522
collapse of the proton motive force in listeria monocytogenes caused by a bacteriocin produced by pediococcus acidilactici.	the effect of pediocin jd, a bacteriocin produced by pediococcus acidilactici jd1-23, on the proton motive force and proton permeability of resting whole cells of listeria monocytogenes scott a was determined. control cells, treated with trypsin-inactivated bacteriocin at a ph of 5.3 to 6.1, maintained a ph gradient and a membrane potential of approximately 0.65 ph unit and 75 mv, respectively. however, these gradients were rapidly dissipated in cells after exposure to pediocin jd, even though n ...	1992	1444365
genomic analysis of pediococcus starter cultures used to control listeria monocytogenes in turkey summer sausage.	the pulsed-field technique of clamped homogeneous electric field electrophoresis was employed to characterize and size genomic dna of three pediocin-producing (ped+) and two non-pediocin-producing (ped-) strains of pediococcus acidilactici. comparison of genomic fingerprints obtained by digestion with the low-frequency-cleavage endonuclease asci revealed identical restriction profiles for four of the five strains analyzed. summation of results for 10 individually sized asci fragments estimated t ...	1992	1444419
identification and characterization of two bacteriocin-producing strains of lactococcus lactis isolated from vegetables.	isolated from mixed salad and fermented carrots, 123 strains of lactic acid bacteria were screened for bacteriocin production. two strains, d53 and 23, identified as lactococcus lactis by dna-dna hybridizations, produced heat stable bacteriocins which were resistant to trypsin and pepsin, but were inactivated by alpha-chymotrypsin and proteinase k. the bacteriocins were active from ph 2 to 9 and inhibited species of listeria, lactobacillus, lactococcus, pediococcus, leuconostoc, carnobacterium, ...	1992	1445757
culture media for non-sporulating gram-positive food spoilage bacteria.	the spoilage association especially of protein-rich foods can be dominated by gram-positive bacteria, notably lactic acid bacteria (lab) which affect vacuum packaged refrigerated processed meats and some dairy products. new food ecosystems are being created by novel packaging and processing technologies, resulting in spoilage associations differing from those previously reported. in addition, improvement in identification methods, allow the detection and isolation of 'novel' bacterial groups, e. ...	1992	1486021
purification and characterization of the hpr protein of pediococcus halophilus.	hpr protein, the ptsh gene product, of the phosphoenolpyruvate:sugar phosphotransferase system of pediococcus halophilus was purified to homogeneity using heat and acid treatments, deae-sepharose cl-6b and hydroxyapatite column chromatographies. the purified protein complemented the hpr activity of the phosphotransferase system in staphylococcus aureus ptsh mutant cell lysate. the molecular weight was estimated as 6,500 by sds polyacrylamide gel electrophoresis. the amino acid sequence of the am ...	1992	1503564
cloning, expression, and nucleotide sequence of genes involved in production of pediocin pa-1, and bacteriocin from pediococcus acidilactici pac1.0.	the production of pediocin pa-1, a small heat-stable bacteriocin, is associated with the presence of the 9.4-kbp plasmid psrq11 in pediococcus acidilactici pac1.0. it was shown by subcloning of psrq11 in escherichia coli cloning vectors that pediocin pa-1 is produced and, most probably, secreted by e. coli cells. deletion analysis showed that a 5.6-kbp sali-ecori fragment derived from psrq11 is required for pediocin pa-1 production. nucleotide sequence analysis of this 5.6-kbp fragment indicated ...	1992	1514784
enhanced control of listeria monocytogenes by in situ-produced pediocin during dry fermented sausage production.	to determine whether pediocin is produced and has effective antilisterial activity during food fermentation, six sausage fermentation trials were conducted with antibiotic-resistant, pediocin-producing (bac+) pediococcus acidilactici pac 1.0 (strr rifr) and an isogenic pediocin-negative (bac-) derivative used as a control. meat was inoculated (ca. 10(5) cfu/g) with a composite of five listeria monocytogenes strains, each electrotransformed with pgk12 (cmr emr). p. acidilactici and l. monocytogen ...	1992	1575489
purification and primary structure of pediocin pa-1 produced by pediococcus acidilactici pac-1.0.	the plasmid-encoded bacteriocin pediocin pa-1, produced by the gram-positive bacterium pediococcus acidilactici strain pac-1.0, was purified to homogeneity. the purified product exhibited antibacterial activity against several gram-positive bacterial strains, including the food pathogen listeria monocytogenes. pediocin pa-1 is a 4629-da peptide with 44 amino acids and two disulfide bonds. the amino acid sequence and arrangement of the disulfide bonds were determined. sequence data were used to c ...	1992	1575516
detection of pediococcus spp. in brewing yeast by a rapid immunoassay.	a membrane immunofluorescent-antibody test was developed to detect diacetyl-producing pediococcus contaminants in brewery pitching yeast (yeast [saccharomyces cerevisiae] slurry collected for reinoculation). centrifugations at 11 and 5,100 x g separate yeast cells from bacteria and concentrate the bacteria, respectively. pelleted bacteria resuspended and trapped on a black membrane filter are reacted with monoclonal antibodies specific for cell surface antigens and then with fluorescein-conjugat ...	1992	1610194
monoclonal antibody-colony immunoblot method specific for isolation of pediococcus acidilactici from foods and correlation with pediocin (bacteriocin) production.	balb/c mice were immunized with broken, heat-killed cells of pediococcus acidilactici h. after murine cell fusions, one monoclonal antibody (mab), ped-2b2, was selected on the basis of its positive reaction with seven of seven strains tested in an enzyme-linked immunosorbent assay with whole cells of p. acidilactici. the mab ped-2b2 did not show any cross-reactions with other lactic-acid bacteria or other gram-positive or gram-negative organisms. sodium dodecyl sulfate-polyacrylamide gel electro ...	1992	1637167
partial characterization of an rpod-like gene of lactococcus lactis subsp. lactis ml3 with a polymerase chain reaction-based approach.	with degenerated oligonucleotide primers for conserved regions of bacterial sigma factor proteins, a 117-bp internal dna fragment of an rpod-like gene of lactococcus lactis subsp. lactis ml3 was amplified by the polymerase chain reaction (pcr). the dna sequence of this pcr product was determined by cycle sequencing, and the deduced amino acid sequence of this internal fragment showed an extensive homology with the known sigma factor sequences from six other microorganisms and present a 13-amino ...	1993	7764136
antimicrobial activity of sulfur dioxide to certain lactic acid bacteria isolated from wines.	this study was conducted to investigate the relationship between ph and the toxicity of sulfur dioxide against homo- and hetero-lactic acid bacteria isolated from american acidic wines. malolactic fermentation was found to be growth-associated in homo- and hetero-lactic strains. the ph of cbb broth had an insignificant effect on the specific death rate of these strains; the concentration of molecular sulfur dioxide had a great effect on the specific death rate and malate degradation. laboratory ...	1993	7982367
enzyme confusion.		1993	8222241
fate of listeria monocytogenes and pediococcal starter cultures during the manufacture of chicken summer sausage.	two formulations of chicken summer sausages [100% hand deboned chicken meat (hdcm) and 85% hdcm and 15% chicken hearts (hdcm-ch)] were prepared with a nonpediocin-producing (ped-) pediococcus acidilactici starter culture and inoculated with 10(4) or 10(7) cfu of a five-strain mixture of listeria monocytogenes/g of batter. sausages were fermented to ph 5.0 (11 h), cooked to an internal temperature of 66.5 c, cold-showered, and stored at 4 c (60 days) and 30 c (7 days). for both formulations and i ...	1993	8234138
microflora of the aerobic preservation of directly brined green olives from hojiblanca cultivar.	new procedures for the preservation stage of ripe olives from hojiblanca cultivar were studied. an aerobic fermentative process was used with initial ph correction (0.3% acetic acid) and various nacl concentrations: 6, 3 and 0% (w/v) in tap water. treatments were carried out at industrial level and the spontaneous changes monitored. at initial salt concentrations of 6 and 3% (w/v) nacl, ph rose progressively, reaching 4.3 at equilibrium maintaining during this period a constant free lactic acidi ...	1993	8244900
detection, identification and characterization of bacteriocin-producing lactic acid bacteria from retail food products.	forty bacteriocin-producing (bac+) lactic acid bacteria (lab) were isolated from food samples purchased from retail supermarkets and local farms. of the 40 bac+ isolates, 18 were isolated from 85 food samples by enrichment (21% isolation rate) whereas eight were obtained from 63 samples by direct plating (13% isolation rate). by direct plating, bac+ lab were detected at levels up to 2.4 x 10(5) cfu/g in ready-to-eat meats. the bac+ isolates were identified by carbohydrate fermentation patterns, ...	1993	8257654
pediocin pa-1, a bacteriocin from pediococcus acidilactici pac1.0, forms hydrophilic pores in the cytoplasmic membrane of target cells.	pediocin pa-1 is a bacteriocin which is produced by pediococcus acidilactici pac1.0. we demonstrate that pediocin pa-1 kills sensitive pediococcus cells and acts on the cytoplasmic membrane. in contrast to its lack of impact on immune cells, pediocin pa-1 dissipates the transmembrane electrical potential and inhibits amino acid transport in sensitive cells. pediocin interferes with the uptake of amino acids by cytoplasmic membrane vesicles derived from sensitive cells, while it is less effective ...	1993	8285666
vancomycin binding to cell walls of non-streptococcal vancomycin-resistant bacteria.	the availability of peptidoglycan for binding to vancomycin was investigated in intrinsically vancomycin-resistant clinical isolates of leuconostoc mesenteroides, lactobacillus viridescens and pediococcus pentosaceus. intrinsic vancomycin resistance expressed by l. mesenteroides and p. pentosaceus was the result of poor binding of vancomycin to native or sds-treated cell walls. pre-exposure of l. viridescens to vancomycin decreased the subsequent binding of vancomycin to the cell walls, suggesti ...	1993	8288496
in vitro activity of ramoplanin against vancomycin-resistant gram-positive organisms.	in vitro activity of ramoplanin, a cyclic lipoglycopeptide, against 92 vancomycin-resistant gram-positive organisms was evaluated. ramoplanin demonstrated potent activity against many highly vancomycin-resistant organisms including enterococci (mics for 90% of strains tested of 0.5 micrograms/ml) and against lactobacillus spp., leuconostoc spp., and pediococcus spp., all of which were inhibited at concentrations of < or = 0.25 micrograms/ml. this drug or a derivative compound merits further inve ...	1993	8328787
characterization and frequency distribution of species of lactic acid bacteria involved in the processing of mawè, a fermented maize dough from benin.	lactic acid bacteria involved in the natural fermentation of both home-produced and commercial mawè were investigated during a 72 h fermentation period. lactobacillus spp. constitute the majority (94%) of the strains of the lactic acid bacteria isolated, among which 89% represent the betabacterium group. they include l. fermentum (biotype cellobiosus) (41%), l. fermentum or l. reuteri (19%), l. brevis (26%), l. confusus (less than 2%), l. curvatus (less than 1%) and l. buchneri (less than 1%). o ...	1993	8347427
pharmacokinetics and in vitro studies of l-leucovorin. comparison with the d and d,l-leucovorin.	the active isomer of leucovorin (lv), lv-6s was compared with the racemic form, lv-6r,s and the inactive form, lv-6r.	1993	8353099
characterization of bacteriocins from enterococcus faecium with activity against listeria monocytogenes.	laboratory cultures and environmental isolates of bacteria were screened for antagonism towards listeria monocytogenes using an agar spot test. seven of the 163 strains that were tested, one streptococcus bovis, one enterococcus casseliflavus, two e. avium and three e. faecium, consistently displayed antilisterial activity. cell-free, ph-neutralized supernatants prepared from the three e. faecium strains (jbl1061, jbl1083 and jbl1351) exhibited strong antilisterial activity against l. monocytoge ...	1993	8398626
microbiological and aromatic characteristics of fermented maize doughs for kenkey production in ghana.	a very uniform microflora was demonstrated in 15 samples of fermented maize dough from different larger commercial production sites. at the advanced stage of fermentation, more than 96% of the bacteria present were morphologically and biochemically uniform, obligatively heterofermentative lactobacilli occurring in concentrations about 10(9) cfu/g. yeast occurred in levels of about 10(6) cfu/g, dominated by candida and saccharomyces spp. studies on microbial successions indicated a selection towa ...	1993	8398627
identification of vancomycin-resistant lactic bacteria isolated from humans.	by using cell morphology, arginine dihydrolase, and gas production in de man, sharp, rogosa broth, 122 isolates of vancomycin-resistant lactic bacteria from humans were assigned to five profiles, allowing us to distinguish pediococcus, homofermentative and heterofermentative lactobacillus, and leuconostoc species. the absence of l-(+)-lactic acid, as detected spectrophotometrically, was confirmatory for leuconostoc species. api 50 chl panels were useful for the identification of lactobacillus sp ...	1993	8408575
purification, partial characterization and plasmid-linkage of pediocin sj-1, a bacteriocin produced by pediococcus acidilactici.	pediococcus acidilactici sj-1, isolated from a naturally-fermented meat product, produced an antibacterial agent active against selected strains of lactobacillus spp., clostridium perfringens and listeria monocytogenes. the agent was bactericidal against sensitive indicators, and sensitive to proteolytic enzymes; it was identified as a bacteriocin, and was designated as pediocin sj-1. it was stable over a wide ph range (3-9), and apparently most stable in the lower part of that range. at ph 3.6, ...	1993	8420920
role of flavin in acetoin production by two bacterial pyruvate oxidases.	escherichia coli pyruvate oxidase (poxec) requires fad both for the oxidative decarboxylation of pyruvate to acetate and co2 and for the formation of acetoin from pyruvate and acetaldehyde. prior work has shown that the catalytic activity (kcat/km) for poxec in the oxidative reaction is stimulated approximately 450-fold by amphiphilic activators. this paper shows that the acetoin reaction does not respond to activation. the fad requirement for acetoin formation can be replaced by 5-deaza-fad and ...	1993	8424670
production and characterization of antibacterial compounds produced by pediococcus damnosus and pediococcus pentosaceus.	the broad-spectrum antibacterial activity exhibited by three pediococcus strains isolated from beer was preliminarily characterized. factors affecting the production rate of bacterial inhibitors were screened and the effects of simultaneous cultivation of lactococcus and pediococcus on the production of inhibitory substances were studied. the antibacterial activity against a range of gram-negative test organisms was not affected by catalase or proteolytic enzymes and was extremely thermotolerant ...	1993	8444642
in vitro activity of rp59500, an injectable streptogramin antibiotic, against vancomycin-resistant gram-positive organisms.	the in vitro activity of rp59500, a streptogramin antibiotic, against 146 clinical isolates of vancomycin-resistant gram-positive bacteria was examined. five strains of the species enterococcus casseliflavus and enterococcus gallinarum, for which the mic of vancomycin was 8 micrograms/ml, were also studied. twenty-eight vancomycin-susceptible strains of enterococcus faecalis and enterococcus faecium were included for comparison. the drug was highly active against leuconostoc spp., lactobacillus ...	1993	8460927
[utilization of lactic bacteria in the control of pathogenic microorganisms in food].	the lactic acid bacteria have the potential to inhibit the growth of pathogenic and spoilage bacteria and the possibility exists of using them to improve the hygienic quality and to extend the shelf-life of different foods. among the many inhibitory substances produced by the lactic acid bacteria, the bacteriocins are of particular interest. it has been the objective of this work to review the bacteriocins produced by lactic acid bacteria from the genera lactococcus, lactobacillus and pediococcu ...	1993	8484916
characterization of bacteriocin produced by pediococcus pentosaceus from wine.	twenty strains of pediococcus pentosaceus isolated from wine were examined for production of bacteriocins. only two of them showed inhibitory activity, ped. pentosaceus n4p against the indicator strains of the same species and n5p against 19 strains of the three genera of lactic acid bacteria from wine. the antimicrobial substance from n5p strains was removed by membrane (0.2 micron) filtration, destroyed by organic solvents and proteolytic enzymes. it was stable for 60 min at 100 degrees c. the ...	1993	8486547
antimicrobial susceptibility of pediococcus spp. and genetic basis of macrolide resistance in pediococcus acidilactici hm3020.	we determined the mics of 28 antimicrobial agents against 36 clinical strains of pediococcus spp. (25 p. acidilactici, 9 p. pentosaceus, and 2 p. urinaeequi strains). penicillin g, imipenem, gentamicin, netilmicin, erythromycin, clindamycin, rifampin, chloramphenicol, daptomycin, and ramoplanin were the most active. all strains of p. acidilactici were susceptible to novobiocin, whereas all isolates of p. pentosaceus were resistant. novobiocin could therefore be helpful for differentiation of the ...	1993	8494376
comparative evaluation of two methods of enumerating enterococci in foods: collaborative study.	two methods of enumerating enterococci in foods were compared in a collaborative study. thirteen laboratories tested four blind duplicate samples containing different levels of enterococci and two negative control samples. freeze-dried mixtures of bacteria were used as simulated food samples. the freeze-dried samples were reconstituted and either spread directly on the surface of slanetz and bartley medium (sb) and incubated at 44 degrees c for 48 h or preincubated in tryptone soya agar at 37 de ...	1993	8494690
alcohol production in submerged cashew pomace.	the variations in the total titratable acidity, hydrogen ion concentration, reducing sugar, soluble solids and alcohol contents of the supernatant of the submerged cashew pomace in a non-aseptic condition were investigated. the crude fibre content of the pomace was also determined during fermentation. at intervals, the changes in microbial load in both the pomace and its supernatant were examined during the 14-day study. fifty four percent of the total soluble solids (tss) was utilised within 7 ...	1993	8506244
expression of glr (muri, dga) gene encoding glutamate racemase in escherichia coli.	the muri (dga) gene of escherichia coli is required for the biosynthesis of d-glutamate, an essential component of bacterial peptidoglycan (doublet, p., van heijetnoort, j., and mengin-lecreulx, d. (1992) j. bacteriol. 174, 5772-5779; dougherty, t. j., thanassi, j. a., and pucci, m. j. (1993) j. bacteriol. 175, 111-116), but its gene product has not been identified. we found that the amino acid sequence of protein deduced from the nucleotide sequence of the open reading frame of muri gene (orf1) ...	1993	7901215
effects of starter cultures on the formation of flavour compounds in dry sausage.	the purpose of this work was to study the impact of starter cultures on the production of flavour compounds in dry sausages. the effect of six starter cultures corresponding to different combinations of lactic acid bacteria (lactobacillus sake l110, pediococcus acidilactici 725, p. pentosaceus 716) and different staphylococcus species (s. carnosus 833, s. warneri 863, s. saprophyticus m31) strains were tested in a total of 30 dry sausages without spices. the analysis of flavour compounds using a ...	1993	22061221
effects of starter cultures on the biochemical characteristics of french dry sausages.	bacterial starter cultures, consisting of lactic acid bacteria (lactobacillus, pediococcus) and staphylococci (staphlylococcus carnosus. s. saprophyticus, s. warneri), have an important effect on the ph, lactate, acetate and free fatty acid contents of sausages. sausages made with l. sake had the lowest ph whereas no change of ph was noticed in the controls and in the sausages inoculated with p. acidilactici. inoculation of s. saprophyticus led to sausages with a high acetate content. lipolysis ...	1993	22061034
effect of chitosan in meat preservation.	the effect of chitosan in meat preservation, including microbiological, chemical, sensory and color qualities, was examined. in liquid medium, chitosan (0·01%) inhibited the growth of some spoilage bacteria such as bacillus subtilis ifo 3025, escherichia coli rb, pseudomonas fragi ifo 3458 and staphylococcus aureus iam 1011. at higher concentrations (0·1 and 1·0%) it inhibited the growth of the meat starter cultures, lactobacillus plantarium iam 1216, pediococcus pentosaceus iam 12296 and microc ...	1994	22059662
lipolysis, proteolysis and formation of volatile components during ripening of a fermented sausage with pediococcus pentosaceus and staphylococcus xylosus as starter cultures.	bacterial growth, formation of acids, lipolysis, proteolysis, fat oxidation, formation of volatile compounds and flavour characteristics were followed during ripening and storage of a fermented sausage. the starter culture used was composed of pediococcus pentosaceus and staphylococcus xylosus. the number of pediococcus sp. increased by 1.5 log cfu/g during the first day of processing and remained constant at this level for 3 weeks. the corresponding initial increase in the numbers of staphyloco ...	1994	22059658
digestion of herring by indigenous bacteria in the minke whale forestomach.	northeastern atlantic minke whales (balaenoptera acutorostrata) have a multichambered stomach system which includes a nonglandular forestomach resembling that of ruminants. bacteria from the forestomachs of herring-eating whales were enumerated and isolated in an anaerobic rumen-like culture medium (m8w medium). the total viable population of anaerobic bacteria ranged from 73 x 10 to 145 x 10/ml of forestomach fluid (n = 4). lactobacillus spp. (19.7%), streptococcus spp. (35.9%), and ruminococcu ...	1994	16349460
the escherichia coli dga (muri) protein shares biological activity and structural domains with the pediococcus pentosaceus glutamate racemase.	the pediococcus pentosaceus glutamate racemase gene product complemented the d-glutamate auxotrophy of escherichia coli wm335. amino acid sequence analysis of the two proteins revealed 28% identity, primarily in six clusters scattered throughout the sequence. further analyses indicated secondary structure similarities between the two proteins. these data support a recent report that the dga (muri) gene product is a glutamate racemase.	1994	7904596
bacterial glutamate racemase has high sequence similarity with myoglobins and forms an equimolar inactive complex with hemin.	glutamate racemase (ec 5.1.1.3), an enzyme of microbial origin, shows significant sequence homology with mammalian myoglobins, in particular in the regions corresponding to the e and f helices, which constitute the heme binding pocket of myoglobins. glutamate racemase binds tightly an equimolar amount of hemin, leading to loss of racemase activity. although this enzyme shows homology with aspartate racemase, the latter does not bind hemin. the glutamate racemase gene of pediococcus pentosaceus h ...	1994	7937852
analysis of the pediocin ach gene cluster from plasmid psmb74 and its expression in a pediocin-negative pediococcus acidilactici strain.	the 3,500-bp pap operon in the 8,877-bp plasmid psmb74 contains a cluster of four genes, papabcd, of which papa encodes prepediocin (a. m. motlagh, m. bukhtiyarova, and b. ray, lett. appl. microbiol. 18:305-312, 1994). the cluster without the promoter was cloned in the shuttle vector phps9. an escherichia coli strain and a pediocin-sensitive pediococcus acidilactici strain transformed with the recombinant plasmid, pmbr1.0, produced pediocin ach. deletion analysis by introducing mutations in the ...	1994	7944372
[microbiological and clinical studies of vancomycin resistant leuconostoc spp. and pediococcus spp. isolated from septicemia patients].	we described three septicemia cases in which blood cultures yielded gram-positive cocci identified as leuconostoc spp. and pediococcus spp. patients were three male adults aged 63 to 71 years with severe underlying diseases, pancreatic cancer, esophageal cancer and diabetes mellitus with chronic renal failure. they had fever and chills at the onsets of septicemia with acute obstructive suppurative cholangitis, acute pneumonia, and infection complicated with invasion sites of esophageal cancer co ...	1994	7963799
on-line enzymatic amplification by substrate cycling in a dual bioreactor with rotation and amperometric detection.	the amplification approach centered on the cycling of two reversibly interconvertible chemical species sequentially participating in two different enzyme-catalyzed reactions (enzymatic amplification by substrate cycling) has been implemented on-line into a continuous-flow/stopped-flow/continuous-flow operation. the implementation is illustrated with the determination of l-lactate in a dual enzyme reactor containing immobilized lactate oxidase (lod, ec 1.1.3.x) to catalyze the oxidation of l-lact ...	1994	7978271
pediocin a, a bacteriocin produced by pediococcus pentosaceus fbb61.	pediocin a, a bacteriocin produced by pediococcus pentosaceus fbb61, was purified and partially characterized. the purification was achieved by dialysis against peg 20,000, butanol extraction and electroendosmotic preparative electrophoresis. the protocol led to a 7843-fold increase in the specific activity, with 3.9% activity recovered. sds-page of pediocin a resulted in a single 80 kda protein band. the antimicrobial compound was sensitive to proteolytic enzymes and heat (10 min at 100 degrees ...	1994	8012591
purification and partial amino acid sequence of curvaticin fs47, a heat-stable bacteriocin produced by lactobacillus curvatus fs47.	curvaticin fs47, a bacteriocin produced by lactobacillus curvatus fs47, is inhibitory to listeria monocytogenes, as well as lactobacillus, pediococcus, enterococcus, and bacillus spp. the bacteriocin was purified by 40% ammonium sulfate precipitation, solid-phase extraction, and reversed-phase high-pressure liquid chromatography. purified curvaticin fs47 was determined to be 4.07 kda by mass spectrometry and was partially sequenced. thirty-one n-terminal amino acids were identified; the curvatic ...	1994	8031103
monoclonal antibody-based enzyme immunoassay for pediocins of pediococcus acidilactici.	monoclonal antibody (mab) r2-ar against pediocin rs2 was developed. mice were immunized for 12 weeks with pediocin rs2 conjugated to a polyacrylamide gel. two hybridoma fusions yielded an mab that in western blots (immunoblots) reacted only with pediocins rs2 and ach (3 kda) from pediococcus acidilactici rs2 and h, respectively, and did not react with any other bacteriocin, including sakacin a from lactobacillus sake lb 706, leuconocin lcm1 from leuconostoc carnosum lm1, nisin from lactococcus l ...	1994	8085814
characterization of the lactobacillus helveticus cnrz32 pepc gene.	sequence analysis of the aminopeptidase c gene (pepc) from lactobacillus helveticus cnrz32 identified a 1,332-nucleotide open reading frame coding for a polypeptide with motifs characteristic of cysteine proteinases. homology to the pepc gene appears to be widely distributed among lactic acid bacteria.	1994	8117086
modification of peptidoglycan precursors is a common feature of the low-level vancomycin-resistant vanb-type enterococcus d366 and of the naturally glycopeptide-resistant species lactobacillus casei, pediococcus pentosaceus, leuconostoc mesenteroides, and enterococcus gallinarum.	the biochemical basis for the acquired or natural resistance of various gram-positive organisms to glycopeptides was studied by high-pressure liquid chromatographic analysis of their peptidoglycan udp-murnac-peptide precursors. in all cases, resistance was correlated with partial or complete replacement of the c-terminal d-ala-d-ala-containing udp-murnac-pentapeptide by a new precursor with a modified c terminus. nuclear magnetic resonance analysis by sequential assignment showed that the new pr ...	1994	8157610
pediococcus pentosaceus catheter-associated infection in an infant with gastroschisis.		1994	8170738
the potential of lactic acid bacteria for the production of safe and wholesome food.	by tradition lactic acid bacteria (lab) are involved in the production of fermented foods. these constitute one quarter of our diet and are characterized by a safe history, certain beneficial health effects, and an extended shelf life when compared with raw materials. the various fermenting substrates are habitats for specific lab that differ in their metabolic potential. the health effects exerted by lab are the following: 1. production of lactic acid and minor amounts of acetic and formic acid ...	1994	8178575
fermentation of fructans by epiphytic lactic acid bacteria.	a total of 712 strains of lactic acid bacteria isolated from forage grasses were studied for their ability to ferment fructans of phlein- as well as inulin-type. only 16 strains utilized phlein and eight of these also fermented inulin. they were identified as lactobacillus paracasei subsp. paracasei, lact. plantarum, lact. brevis and pediococcus pentosaceus. in the species lact. paracasei subsp. paracasei, all strains gave positive results, whereas the other positive strains possessed unique pro ...	1994	8200866
simple method for extracting plasmid dna from lactic acid bacteria.	rapid screening and large-scale plasmid dna isolation procedures are described for lactic acid bacteria, using glass beads to break cells. the rapid screening procedure allows one to obtain plasmid dna pellets in less than 1 h. this method has been successfully tested on various bacteria from the genera lactococcus, leuconostoc, lactobacillus, pediococcus, streptococcus, enterococcus and propionibacterium. this procedure yields plasmid dna with minor chromosomal and plasmid dna-degraded form con ...	1994	7764702
complete nucleotide sequence of psmb 74, a plasmid encoding the production of pediocin ach in pediococcus acidilactici.	several pediococcus acidilactici strains produce a plasmid-encoded bacteriocin, pediocin ach. previous studies have shown that this plasmid, designated as psmb 74, encodes genes associated with the production of prepediocin, its post-translation processing to pediocin ach, transmembrane translocation of these molecules, and immunity of producer cells against pediocin ach. we report here the complete nucleotide sequence of psmb 74. the plasmid has a total of 8877 bp. four genes have been located ...	1994	7764941
simple method of purification and sequencing of a bacteriocin produced by pediococcus acidilactici ul5.	a bacteriocin produced by a strain of pediococcus acidilactici was successfully purified sequentially by acid extraction (at ph 2) and reverse-phase high-performance liquid chromatography (hplc). cell extracts of derivative strains deficient in bacteriocin production exhibited a similar hplc elution profile to the active extracts except for the two peaks containing bacteriocin activity. the sequence of the antibacterial peptide consisted of 44 amino acid residues of which 42 were identified, and ...	1994	7822227
inhibition of spoilage and food-borne pathogens by lactic acid bacteria isolated from fermenting tef (eragrostis tef) dough.	a study was carried out at the department of biology, addis abeba university, in 1991 to determine the inhibitory potential of fermenting tef and the lactic acid bacteria isolated from fermenting tef dough on salmonella spp., pseudomonas aeruginosa, klebsiella spp., bacillus cereus and staphylococcus aureus. the test bacteria grew in the fermenting tef uptill 30 hr or till the ph dropped to 4.7. thereafter, growth was inhibited and decreases in population were apparent. the results showed that t ...	1994	7835350
characterisation of lactic acid bacteria isolated from naturally fermented greek dry salami.	a total of 348 lactic acid bacteria isolated from five batches of naturally fermented dry salami at various stages of ripening were characterised. the majority of the strains were assigned to two main phylogenetic groups of species: (i) the psychrotrophic, formerly called atypical, meat streptobacteria (169 strains) and (ii) a new genus weissella (120), which was recently proposed (collins et al., 1993) to include leuconostoc paramesenteroides and some other closely related species. meat strepto ...	1994	7848780
survival and growth of selected pathogens in fermented kocho (ensete ventricosum).	a study was conducted to investigate the antagonistic potential of fermented kocho, aqueous extract of fermented kocho and spent media from lactic acid bacteria (lab) isolated from it on salmonella sp, pseudomonas aeruginosa, klebsiella sp, bacillus cereus and staphylococcus aureus. fermented kocho (ph 4.3) inhibited growth of the test bacteria soon after their introduction into the food. the spent media from all of the four lab, isolated from fermented kocho, namely pediococcus sp, leuconostoc ...	1994	7867544
influence of ph, salt and nitrite on the heme-dependent catalase activity of lactic acid bacteria.	a screening of commercial starter cultures used for the production of dry sausage showed a maximum heme-dependent catalase activity in the range of 60 mumol/l hematin for lactobacillus sake, lactobacillus plantarum, lactobacillus pentosus and pediococcus acidilactici. pseudocatalase activity was not detected. in standard dry sausage production, 2-3% (w/w) nitrite salt (0.6% sodium nitrite per 100 g nacl) is normally added, which corresponds to 4-6% salt in the water phase. in vitro experiments w ...	1994	7703013
effect of three commercial starters on growth of staphylococcus aureus and enterotoxins (a-d) and thermonuclease production in broth.	the growth of four enterotoxigenic staphylococcus aureus strains was partially inhibited by three commercial starters used in the meat sausage industry when grown in apt broth at 30 degrees c statically. starter sp318 (a mixture of selected strains of lactobacillus sake, pediococcus pentosaceus and staphylococcus xylosus) showed the most inhibitory activity. staphylococcal enterotoxins (a, b, c1 and d) synthesis was totally inhibited by the growth of the three starters, whereas staphylococcal th ...	1994	7703025
pucl287 plasmid from tetragenococcus halophila (pediococcus halophilus) atcc 33315 represents a new theta-type replicon family of lactic acid bacteria.	a cryptic plasmid, pucl287, was isolated from tetragenococcus halophila (pediococcus halophilus) atcc 33315. it had a theta-type mechanism of replication in its natural host. its minimal replicon, rep287, was isolated on a 1.6-kb ecori fragment. the rep287 host range included the genera pediococcus, enterococcus, lactobacillus and leuconostoc but not genus lactococcus. plasmids hybridizing to pucl287 are rare among lactic acid bacteria. as assessed by hybridization, rep287 is dissimilar to pam b ...	1995	7750734
production and stability of pediocin n5p in grape juice medium.	the production and stability of pediocin n5p from pediococcus pentosaceus, isolated from wine, were examined in grape juice medium. maximum growth and higher titre (4000 u ml-1) were observed at a initial ph of 7.5 and 30 degrees c. the activity of the inhibitory substance was stable between ph values from 2.0 to 5.0 at 4 degrees and 30 degrees c. at ph 10.0 it was completely inactivated. when submitted to 30 min at 80 degrees, 100 degrees and 115 degrees c, maximal stability was observed at ph ...	1995	7759379
microflora of murcha: an amylolytic fermentation starter.	murcha is a traditional starter, used commonly in darjeeling hills and sikkim in india to ferment a variety of starchy substrates in order to produce sweet-sour alcoholic beverages, called jnards. murcha cakes are mildly acidic (ph 5.2) and contain 13% w/w moisture and 0.7% w/w ash (dry weight basis). a total of 194 bacterial, 190 yeast and 80 mould strains were isolated from 30 samples of murcha. the counts (cfu/g fresh weight) of micro-organisms in the samples were 2.0 x 10(7) to 4.2 x 10(8) f ...	1995	7476555
biodiversity of lactic acid bacteria from food-related ecosystems.	the diversity of lactic acid bacteria within a variety of food-related ecosystems was assessed and the strain as well as environment specific characteristics were investigated. the strains (108 in toto) were isolated from plant material, traditional fermented foods, dried marine algae, sea food, fungi as well as spoiled foods and beverages, and all were characterized to genus level. lactobacillus strains dominated all ecosystems and contributed to 65% of the isolates while a further 13% consiste ...	1995	7476564
genetic organization of a small cryptic plasmid of helicobacter pylori.	a 2.9-kb cryptic plasmid of helicobacter pylori (hp), phel1, was isolated and the complete nucleotide (nt) sequence was determined. an open reading frame (orf1) was identified encoding a putative polypeptide of 63,709 da, the existence and correct size of which was confirmed by t7 promoter expression analysis. the orf1 sequence showed strong amino-acid sequence identity to a recently identified putative orf1 protein of a cryptic hp plasmid, phpm180, and significant homologies to putative rep pro ...	1995	7489910
in vitro fructooligosaccharide utilization and inhibition of salmonella spp. by selected bacteria.	in vitro experiments were conducted to determine: 1) inhibitory capacities of potential direct-fed microbial bacteria against salmonella serotypes; and 2) the ability of bifidobacterium bifidum, enterococcus faecium, lactobacillus casei, lactococcus lactis, pediococcus sp., and salmonella spp. to grow in media containing fructooligosaccharides (fos-50 or fos pure formulation) as the only carbohydrate source. thirteen bacteria (two strains of bacillus coagulans, bacillus licheniformis, bacillus s ...	1995	7501585
pediococcus acidilactici ldhd gene: cloning, nucleotide sequence, and transcriptional analysis.	the gene encoding d-lactate dehydrogenase was isolated on a 2.9-kb insert from a library of pediococcus acidilactici dna by complementation for growth under anaerobiosis of an escherichia coli lactate dehydrogenase and pyruvate-formate lyase double mutant. the nucleotide sequence of ldhd encodes a protein of 331 amino acids (predicted molecular mass of 37,210 da) which shows similarity to the family of d-2-hydroxyacid dehydrogenases. the enzyme encoded by the cloned fragment is equally active on ...	1995	7539419
additives containing bacteria and enzymes for alfalfa silage.	first-cutting alfalfa was wilted, harvested from alternate rows, left untreated or treated with additives containing lactic acid bacteria and enzymes (cellulase, amylase, and pectinase), and ensiled in bag silos. inoculation increased lactic acid bacteria from 5 x 10(4) to 1 x 10(6) cfu/g of forage. because treatments were bagged consecutively, the dm of treated silages was higher than that of untreated silage. however, after 4 d of ensiling, the ph of treated silage, about 4.3, was lower than t ...	1995	7540187
the formation of biogenic amines by fermentation organisms.	a total of 523 strains representing 35 species related to food fermentation organisms of practical importance were investigated for their potential for formation of biogenic amines (ba). the investigation was performed with resting cells in phosphate buffer (ph 5.5) and the formation of the following bas was followed: putrescine, cadaverine, histamine, tyramine and 2-phenylethylamine. no potential was observed in species of lactococcus, leuconostoc, pediococcus, streptococcus and several lactoba ...	1995	7571871
expression of lactococcin a and pediocin pa-1 in heterologous hosts.	pediocin pa-1 production, immunity and secretion are specified by a cluster of four genes in pediococcus acidilactici pac1.0. the production by, secretion of, and immunity to lactococcin a of lactococcus lactis are also determined by four genes. here, expression of the pediocin operon in lactococcus lactis is reported, which could only be achieved by placing it under control of a lactococcal promoter. expression of the lactococcin a operon in pediococcus is also described: recombinant clones of ...	1995	7576505
in vitro inhibition of helicobacter pylori nctc 11637 by organic acids and lactic acid bacteria.	in this study the effects of both ph and organic acids on helicobacter pylori nctc 11637 were tested. lactobacillus acidophilus, lact. casei, lact. bulgaricus, pediococcus pentosaceus and bifidobacterium bifidus were assayed for their lactic acid production, ph and inhibition of h. pylori growth. a standard antimicrobial plate well diffusion assay was employed to examine inhibitory effects. lactic, acetic and hydrochloric acids demonstrated inhibition of h. pylori growth in a concentration-depen ...	1995	7592140
effect of pediococcus pentosaceus fbb61, pediocin a producer strain, in caecal fermentations.	the objective of this study was to investigate the effect of pediocin a in in vitro caecal fermentations. pediococcus pentosaceus fbb61, pediocin a producer (bac+) and its isogenic mutant (bac-) ped. pentosaceus fbb61-2 were added to fermentation vessels. pediocin a did not alter the normal activity of caecal microflora. nevertheless, the presence of pediocin a producer strain reduced proteolysis compared to the mutant strain as indicated by ammonia concentrations (p < 0.05), and isobutyric and ...	1995	7615417
isolation and characterization of pediocin l50, a new bacteriocin from pediococcus acidilactici with a broad inhibitory spectrum.	lactic acid bacteria were isolated from spanish dry-fermented sausages and screened for bacteriocin production. about 10% of the isolates produced antimicrobial substances when grown on solid media, but only 2% produced detectable activity in liquid media. strain l50, identified as pediococcus acidilactici, showed the strongest inhibitory activity and was active against members of all of the gram-positive genera tested. the strain produced a heat-stable bacteriocin when grown at 8 to 32 degrees ...	1995	7618877
inhibitory effect of selected lactic acid bacteria on microflora associated with ready-to-use vegetables.	the addition of selected lactic acid bacteria strains had a remarkable inhibitory effect on the growth dynamics of microflora associated with ready-to-use vegetables, during refrigerated storage. in particular, coliforms and enterococci were strongly reduced or eliminated from the products from the third day of storage. lactobacillus casei strains proved more effective than pediococci. the use of lactic cultures able to produce bacteriocins and to grow at low temperatures could be a useful tool ...	1995	7639993
udp-n-acetylmuramyl-l-alanine functions as an activator in the regulation of the escherichia coli glutamate racemase activity.	d-glutamate is an essential component of the bacterial peptidoglycan. in escherichia coli, the biosynthesis of d-glutamate is catalyzed by a glutamate racemase (encoded by the dga gene) and is regulated by udp-n-acetylmuramyl-l-alanine [doublet et al. (1994) biochemistry 33, 5285], a bacterial peptidoglycan subunit precursor. investigation was conducted to elucidate the interaction between the enzyme and its regulator. whole and n-terminal truncated enzymes, encoded by individual constructs cont ...	1995	7873525
cloning, nucleotide sequence, and transcriptional analysis of the pediococcus acidilactici l-(+)-lactate dehydrogenase gene.	recombinant plasmids containing the pediococcus acidilactici l-(+)-lactate dehydrogenase gene (ldhl) were isolated by complementing for growth under anaerobiosis of an escherichia coli lactate dehydrogenase-pyruvate formate lyase double mutant. the nucleotide sequence of the ldhl gene predicted a protein of 323 amino acids showing significant similarity with other bacterial l-(+)-lactate dehydrogenases and especially with that of lactobacillus plantarum. the ldhl transcription start points in p. ...	1995	7887607
the lysins of bacteriophages infecting lactic acid bacteria.	this short review highlights the complete absence of literature on lysins of bacteriophages infecting species like s. salivarius subsp. thermophilus, pediococcus and leuconostoc species, l. helveticus, l. acidophilus, l. plantarum and l. brevis, which are also widely used in the dairy industry. the lysins described share some similar biochemical characteristics: optimal ph and temperature, site of hydrolysis inside the peptidoglycan, and some activators and inhibitors. the cloning of the genes e ...	1995	7766123
evaluation of three disk tests for identification of enterococci, leuconostocs, and pediococci.	simple rapid tests for presumptive identification of catalase-negative non-beta-hemolytic cocci (i.e., enterococci, leuconostocs, and pediococci) have not previously been available. seven hundred thirty-four strains of aerobic and facultatively anaerobic, catalase-negative, non-beta-hemolytic gram-positive cocci were tested for susceptibility to vancomycin (vans) by a screening procedure and production of leucine aminopeptidase (lapase) and pyrrolidonylarylamidase (pyrase) in disk tests. three u ...	1995	7790454
staphylococcus haemolyticus contains two d-glutamic acid biosynthetic activities, a glutamate racemase and a d-amino acid transaminase.	two d-glutamic acid biosynthetic activities, glutamate racemase and d-amino acid transaminase, have been described previously for bacteria. to date, no bacterial species has been reported to possess both activities. genetic complementation studies using escherichia coli wm335, a d-glutamic acid auxotroph, and cloned chromosomal dna fragments from staphylococcus haemolyticus revealed two distinct dna fragments containing open reading frames which, when present, allowed growth on medium without ex ...	1995	7814322
influences on the antimicrobial activity of surface-adsorbed nisin.	the efficacy of the antimicrobial peptide nisin was examined after adsorption to silica surfaces. three protocols were used to evaluate nisin's activity against adhered cells of listeria monocytogenes: bioassay using pediococcus pentosaceous fbb 61-2 as the sensitive indicator strain; visualization and enumeration of cells by microscopic image analysis; and viability of adhered cells as determined by lodonitrotetrazolium violet uptake and crystallization. the activity of adsorbed nisin was highl ...	1995	8519481
genetic and biochemical characterization of the lactobacillus delbrueckii subsp. lactis bacteriophage ll-h lysin.	ll-h, a virulent phage of lactobacillus delbrueckii subsp. lactis, produces a peptidoglycan-degrading enzyme, mur, that is effective on l. delbrueckii, lactobacillus acidophilus, lactobacillus helveticus, and pediococcus damnosus cell walls. in this study, the ll-h gene mur was cloned into escherichia coli, its nucleotide sequence was determined, and the enzyme produced in e. coli was purified and biochemically characterized. mur was purified 112-fold by means of ammonium sulfate precipitation a ...	1995	8526515
functional analysis of the pediocin operon of pediococcus acidilactici pac1.0: pedb is the immunity protein and pedd is the precursor processing enzyme.	the bacteriocin pediocin pa-1 operon of pediococcus acidilactici pac1.0 encompasses four genes: peda, pedb, pedc and pedd. transcription of the operon results in the formation of two overlapping transcripts, probably originating from a single promoter upstream of peda. the major transcript comprises peda, pedb, and pedc, while a minor transcript encompasses all of these genes and pedd. by deletion analysis and overexpression of pedb in pediococcus pentosaceus we demonstrate that this gene encode ...	1995	8559070
identification of the replication region of lactobacillus acidophilus plasmid pla103.	the structure of the region necessary for replication of the plasmid pla103 from lactobacillus acidophilus tk8912 has been characterized. sequence analysis revealed that the replication region contained an open reading frame (orfa) encoding a 282-amino acid peptide preceded by a 22-bp tandem repeat sequence region. the predicted orfa protein showed homology to the replication protein of a plasmid from pediococcus halophilus. the plasmid containing the repeat sequence region preceding orfa was ab ...	1995	8566697
the antimicrobial activity of lactic acid bacteria from fermented maize (kenkey) and their interactions during fermentation.	a total of 241 lactic acid bacteria belonging to lactobacillus plantarum, pediococcus pentosaceus, lactobacillus fermentum/reuteri and lactobacillus brevis from various processing stages of maize dough fermentation were investigated. results indicated that each processing stage has its own microenvironment with strong antimicrobial activity. about half of the lact. plantarum and practically all of the lact. fermentum/reuteri investigated were shown to inhibit other gram-positive and gram-negativ ...	1995	8567490
polysaccharide production by pediococcus pentosaceus from wine.	two polymer-forming strains of pediococcus pentosaceus from argentinian wines were grown under different conditions. polysaccharides were produced independently of carbon source at the early logarithmic phase of growth. with the presence of ethanol and so2, the specific polymers production was greater. the polysaccharides comprised of glucose, fructose and galactose, with predominant linkages of alpha-1,4- and alpha-1,6-glucosidic with a ratio 1:1. they contained approximately 5500 hexose molecu ...	1995	8579981
identification, classification, and clinical relevance of catalase-negative, gram-positive cocci, excluding the streptococci and enterococci.	several new genera and species of gram-positive, catalase-negative cocci that can cause infections in humans have been described. although these bacteria were isolated in the clinical laboratory, they were considered nonpathogenic culture contaminants and were not thought to be the cause of any diseases. isolation of pure cultures of these bacteria from normally sterile sites has led to the conclusion that these bacteria can be an infrequent cause of infection. this review describes the new bact ...	1995	8665466
characterization and replication mode determination of the minimal replicon of tetragenococcus halophila atcc33315 plasmid pucl287.	pucl287 is a cryptic plasmid of tetragenococcus halophila (formerly pediococcus halophilus) atcc33315 of relatively small size (8.7 kb). its minimal replicon was located on a 1235 bp mami-ecori fragment. this minimal replicon contains a non-translated region, followed by a gene encoding a putative 311 amino acid protein. deletion experiments showed that the non-translated region corresponds to the replication origin. determination of the replication mode was carried out in enterococcus faecalis ...	1995	8824766
effects of a bacterial hay preservative (pediococcus pentosaceus) on hay under experimental storage conditions.	the abundant growth of molds and thermophilic actinomycetes in stored hay decreases its quality and can be hazardous for the producer who inhales these contaminants when the moldy hay is fed in closed barns. these microbes are responsible for a respiratory disease called farmer's lung. products, including bacterial cultures that can be inoculated in hay, are available to prevent hay deterioration by molds and bacteria. the aim of this study was to verify the effectiveness of pediococcus pentosac ...	1995	16535180
dried sausages fermented with staphylococcus xylosus at different temperatures and with different ingredient levels - part i. chemical and bacteriological data.	sausages with added staphylococcus xylosus were fermented at different temperatures and with different added levels of salt, glucose, nitrite, nitrate and pediococcus pentosaceus in accordance with a six factor fractional design. the numbers of surviving staphylococcus xylosus, lactic acid bacteria, ph, free fatty acids and residual amounts of nitrite and nitrate were measured. the effects of temperature and different ingredients on the chemical and bacterial data were tested using multiple line ...	1995	22060168
dried sausages fermented with staphylococcus xylosus at different temperatures and with different ingredient levels - part iii. sensory evaluation.	sausages with added staphylococcus xylosus were fermented at different temperatures and with different added levels of salt, glucose, nitrite, nitrate and pediococcus pentosaceus in accordance with a six factor fractional design. the odour of the sausages was evaluated by a quantitative descriptive method with ten descriptors and by gas chromatography olfactometry. the sensory profile was correlated to the experimental design and the volatile compounds by partial least squares analysis. also, th ...	1995	22060170
dried sausages fermented with staphylococcus xylosus at different temperatures and with different ingredient levels - part ii. volatile components.	sausages, with added staphylococcus xylosus, were fermented at different temperatures and with different added levels of salt, glucose, nitrite, nitrate and pediococcus pentosaceus in accordance with a six factor fractional design. the volatile compounds from the sausages were collected by dynamic headspace sampling and quantified and identified by gas chromatography and gas chromatography-mass spectrometry. the effects of temperature and different ingredients on the levels of individual volatil ...	1995	22060169
development and characterization of lactose-positive pediococcus species for milk fermentation.	bacteriophages against streptococcus thermophilus are a growing problem in the italian cheese industry. one possible control method involves replacing s. thermophilus in mozzarella starter blends with lactic acid bacteria from a different genus or species. in this study, we evaluated lactose-positive pediococci for this application. because we could not identify any commercially available pediococci with fast acid-producing ability in milk, we transformed pediococcus pentosaceus atcc 25744, p. p ...	1996	16535280